LOCUS       NM_172669               5329 bp    mRNA    linear   ROD 17-DEC-2024
DEFINITION  Mus musculus autophagy/beclin 1 regulator 1 (Ambra1), transcript
            variant 1, mRNA.
ACCESSION   NM_172669
VERSION     NM_172669.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5329)
  AUTHORS   Xu,W., Hua,Z., Wang,Y., Tang,W., Ou,W., Liu,F., Yang,Y., Ding,W.,
            Wang,Z., Cui,L., Ge,W., Gu,Y., Wang,X., Chen,Y., Liu,C.Y. and Du,P.
  TITLE     AMBRA1 promotes intestinal inflammation by antagonizing PP4R1/PP4c
            mediated IKK dephosphorylation in an autophagy-independent manner
  JOURNAL   Cell Death Differ 31 (5), 618-634 (2024)
   PUBMED   38424148
  REMARK    GeneRIF: AMBRA1 promotes intestinal inflammation by antagonizing
            PP4R1/PP4c mediated IKK dephosphorylation in an
            autophagy-independent manner.
REFERENCE   2  (bases 1 to 5329)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 5329)
  AUTHORS   Frias,A., Di Leo,L., Antoranz,A., Nazerai,L., Carretta,M.,
            Bodemeyer,V., Pagliuca,C., Dahl,C., Claps,G., Mandelli,G.E.,
            Andhari,M.D., Pacheco,M.P., Sauter,T., Robert,C., Guldberg,P.,
            Madsen,D.H., Cecconi,F., Bosisio,F.M. and De Zio,D.
  TITLE     Ambra1 modulates the tumor immune microenvironment and response to
            PD-1 blockade in melanoma
  JOURNAL   J Immunother Cancer 11 (3) (2023)
   PUBMED   36868570
  REMARK    GeneRIF: Ambra1 modulates the tumor immune microenvironment and
            response to PD-1 blockade in melanoma.
REFERENCE   4  (bases 1 to 5329)
  AUTHORS   Ramirez-Pardo,I., Villarejo-Zori,B., Jimenez-Loygorri,J.I.,
            Sierra-Filardi,E., Alonso-Gil,S., Marino,G., de la Villa,P.,
            Fitze,P.S., Fuentes,J.M., Garcia-Escudero,R., Ferrington,D.A.,
            Gomez-Sintes,R. and Boya,P.
  TITLE     Ambra1 haploinsufficiency in CD1 mice results in metabolic
            alterations and exacerbates age-associated retinal degeneration
  JOURNAL   Autophagy 19 (3), 784-804 (2023)
   PUBMED   35875981
  REMARK    GeneRIF: Ambra1 haploinsufficiency in CD1 mice results in metabolic
            alterations and exacerbates age-associated retinal degeneration.
REFERENCE   5  (bases 1 to 5329)
  AUTHORS   Pignataro,A., Krashia,P., De Introna,M., Nobili,A., Sabetta,A.,
            Stabile,F., La Barbera,L., D'Addario,S.L., Ventura,R., Cecconi,F.,
            D'Amelio,M. and Ammassari-Teule,M.
  TITLE     Chemogenetic rectification of the inhibitory tone onto hippocampal
            neurons reverts autistic-like traits and normalizes local
            expression of estrogen receptors in the Ambra1+/- mouse model of
            female autism
  JOURNAL   Transl Psychiatry 13 (1), 63 (2023)
   PUBMED   36804922
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 5329)
  AUTHORS   Van Humbeeck,C., Cornelissen,T., Hofkens,H., Mandemakers,W.,
            Gevaert,K., De Strooper,B. and Vandenberghe,W.
  TITLE     Parkin interacts with Ambra1 to induce mitophagy
  JOURNAL   J Neurosci 31 (28), 10249-10261 (2011)
   PUBMED   21753002
  REMARK    GeneRIF: These data identify interaction of Parkin with Ambra1 as a
            key mechanism for induction of the final clearance step of
            Parkin-mediated mitophagy.
REFERENCE   7  (bases 1 to 5329)
  AUTHORS   Herrera,V.L., Decano,J.L., Steffen,M. and Ruiz-Opazo,N.
  TITLE     Autophagy: insights from DEspR-deficiency and haploinsufficiency
  JOURNAL   Autophagy 5 (2), 259-262 (2009)
   PUBMED   19139631
REFERENCE   8  (bases 1 to 5329)
  AUTHORS   Fimia,G.M., Stoykova,A., Romagnoli,A., Giunta,L., Di Bartolomeo,S.,
            Nardacci,R., Corazzari,M., Fuoco,C., Ucar,A., Schwartz,P.,
            Gruss,P., Piacentini,M., Chowdhury,K. and Cecconi,F.
  TITLE     Ambra1 regulates autophagy and development of the nervous system
  JOURNAL   Nature 447 (7148), 1121-1125 (2007)
   PUBMED   17589504
  REMARK    GeneRIF: Ambra1 functional deficiency in mouse embryos leads to
            severe neural tube defects associated with autophagy impairment,
            accumulation of ubiquitinated proteins, unbalanced cell
            proliferation and excessive apoptotic cell death
REFERENCE   9  (bases 1 to 5329)
  AUTHORS   Okazaki,N., Kikuno,R., Ohara,R., Inamoto,S., Koseki,H., Hiraoka,S.,
            Saga,Y., Nagase,T., Ohara,O. and Koga,H.
  TITLE     Prediction of the coding sequences of mouse homologues of KIAA
            gene: III. the complete nucleotide sequences of 500 mouse
            KIAA-homologous cDNAs identified by screening of terminal sequences
            of cDNA clones randomly sampled from size-fractionated libraries
  JOURNAL   DNA Res 10 (4), 167-180 (2003)
   PUBMED   14621295
REFERENCE   10 (bases 1 to 5329)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CJ150588.1, AK083596.1, AK129433.1 and BC032293.1.
            
            On Jan 24, 2007 this sequence version replaced NM_172669.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC054082.2, AK155513.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-44                CJ150588.1         1-44
            45-1648             AK083596.1         2-1605
            1649-4715           AK129433.1         913-3979
            4716-5329           BC032293.1         4366-4979
FEATURES             Location/Qualifiers
     source          1..5329
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 50.63 cM"
     gene            1..5329
                     /gene="Ambra1"
                     /gene_synonym="2310079H06Rik; A130023A14; D030051N19Rik;
                     mKIAA1736"
                     /note="autophagy/beclin 1 regulator 1"
                     /db_xref="GeneID:228361"
                     /db_xref="MGI:MGI:2443564"
     exon            1..242
                     /gene="Ambra1"
                     /gene_synonym="2310079H06Rik; A130023A14; D030051N19Rik;
                     mKIAA1736"
                     /inference="alignment:Splign:2.1.0"
     exon            243..496
                     /gene="Ambra1"
                     /gene_synonym="2310079H06Rik; A130023A14; D030051N19Rik;
                     mKIAA1736"
                     /inference="alignment:Splign:2.1.0"
     CDS             362..4264
                     /gene="Ambra1"
                     /gene_synonym="2310079H06Rik; A130023A14; D030051N19Rik;
                     mKIAA1736"
                     /note="isoform 1 is encoded by transcript variant 1;
                     activating molecule in BECN1-regulated autophagy protein
                     1; activating molecule in Beclin1-regulated autophagy"
                     /codon_start=1
                     /product="activating molecule in BECN1-regulated autophagy
                     protein 1 isoform 1"
                     /protein_id="NP_766257.3"
                     /db_xref="CCDS:CCDS38179.1"
                     /db_xref="GeneID:228361"
                     /db_xref="MGI:MGI:2443564"
                     /translation="MKVVPEKNAVRILWGRERGTRAMGAQRLLQELVEDKTRWMKWEG
                     KRVELPDSPRSTFLLAFSPDRTLLASTHVNHNIYITEVKTGKCVHSLIGHRRTPWCVT
                     FHPTISGLIASGCLDGEVRIWDLHGGSESWFTDSNNAIASLAFHPTAQLLLIATANEI
                     HFWDWSRREPFAVVKTASEMERVRLVRFDPLGHYLLTAIVNPSNQQGDDEPEIPIDGT
                     ELSHYRQRALLQSQPVRRTPLLHNFLHMLSSRSSGIQVGEQSTVQDSATPSPPPPPPQ
                     PSTERPRTSAYIRLRQRVSYPTTVECCQHPGILCLCSRCAGTRVPSLLPHQDSVPPAS
                     ARATTPSFSFVQTEPFHPPEQASSTQQDQGLLNRPSAFSTVQSSTAGNTLRNLSLGPT
                     RRSLGGPLSSHPSRYHRELAPGLTGSEWTRTVLTLNSRSEVESMPPPRTSASSVSLLS
                     VLRQQEGGSQASVYTSATEGRGFPSSGLATESDGGNGSSQNNSGSIRHELQCDLRRFF
                     LEYDRLQELDQSLSGETPQTQQAQEMLNNNIESERPGPSHLPTPHSSENNSNLSRGHL
                     NRCRACHNLLTFNNDTLRWERTTPNYSSGEASSSWHVSTTFEGMPPSGNQLPPLERTE
                     GQMPSSSRLELSSSASSQEERTVGVAFNQETGHWERIYTQSSRSGTVSQEALHQDMPE
                     ESSEEDSLRRRLLESSLISLSRYDGAGSREHPIYPDPARLSPAAYYAQRMIQYLSRRD
                     SIRQRSMRYQQNRLRSSTSSSSSDNQGPSVEGTDLEFEDFEDNGDRSRHRAPRNARMS
                     APSLGRFVPRRFLLPEYLPYAGIFHERGQPGLATHSSVNRVLAGAVIGDGQSAVASNI
                     ANTTYRLQWWDFTKFDLPEISNASVNVLVQNCKIYNDASCDISADGQLLAAFIPSSQR
                     GFPDEGILAVYSLAPHNLGEMLYTKRFGPNAISVSLSPMGRYVMVGLASRRILLHPST
                     EHMVAQVFRLQQAHGGETSMRRVFNVLYPMPADQRRHVSINSARWLPEPGLGLAYGTN
                     KGDLVICRPEALNSGIEYYWDQLSETVFTVHSSSRSSERPGTSRATWRTDRDMGLMNA
                     IGLQPRNPTTSVTSQGTQTLALQLQNAETQTEREEEEPGAASSGPGEGEGSEYGGSGE
                     DALSRIQRLMAEGGMTAVVQREQSTTMASMGGFGNNIIVSHRIHRSSQTGTESGAART
                     SSPQPSTSRGLPSEPGQLAERALSPRTASWDQPSTSGRELPQPALSSSSPVPIPVPLA
                     SNEGPTMHCNVTNNSHLPEGDGSNRGEAAGPSGEPQNR"
     exon            497..555
                     /gene="Ambra1"
                     /gene_synonym="2310079H06Rik; A130023A14; D030051N19Rik;
                     mKIAA1736"
                     /inference="alignment:Splign:2.1.0"
     exon            556..739
                     /gene="Ambra1"
                     /gene_synonym="2310079H06Rik; A130023A14; D030051N19Rik;
                     mKIAA1736"
                     /inference="alignment:Splign:2.1.0"
     exon            740..912
                     /gene="Ambra1"
                     /gene_synonym="2310079H06Rik; A130023A14; D030051N19Rik;
                     mKIAA1736"
                     /inference="alignment:Splign:2.1.0"
     exon            913..979
                     /gene="Ambra1"
                     /gene_synonym="2310079H06Rik; A130023A14; D030051N19Rik;
                     mKIAA1736"
                     /inference="alignment:Splign:2.1.0"
     exon            980..2436
                     /gene="Ambra1"
                     /gene_synonym="2310079H06Rik; A130023A14; D030051N19Rik;
                     mKIAA1736"
                     /inference="alignment:Splign:2.1.0"
     exon            2437..2523
                     /gene="Ambra1"
                     /gene_synonym="2310079H06Rik; A130023A14; D030051N19Rik;
                     mKIAA1736"
                     /inference="alignment:Splign:2.1.0"
     exon            2524..2703
                     /gene="Ambra1"
                     /gene_synonym="2310079H06Rik; A130023A14; D030051N19Rik;
                     mKIAA1736"
                     /inference="alignment:Splign:2.1.0"
     exon            2704..2784
                     /gene="Ambra1"
                     /gene_synonym="2310079H06Rik; A130023A14; D030051N19Rik;
                     mKIAA1736"
                     /inference="alignment:Splign:2.1.0"
     exon            2785..2885
                     /gene="Ambra1"
                     /gene_synonym="2310079H06Rik; A130023A14; D030051N19Rik;
                     mKIAA1736"
                     /inference="alignment:Splign:2.1.0"
     exon            2886..2996
                     /gene="Ambra1"
                     /gene_synonym="2310079H06Rik; A130023A14; D030051N19Rik;
                     mKIAA1736"
                     /inference="alignment:Splign:2.1.0"
     exon            2997..3185
                     /gene="Ambra1"
                     /gene_synonym="2310079H06Rik; A130023A14; D030051N19Rik;
                     mKIAA1736"
                     /inference="alignment:Splign:2.1.0"
     exon            3186..3340
                     /gene="Ambra1"
                     /gene_synonym="2310079H06Rik; A130023A14; D030051N19Rik;
                     mKIAA1736"
                     /inference="alignment:Splign:2.1.0"
     exon            3341..3480
                     /gene="Ambra1"
                     /gene_synonym="2310079H06Rik; A130023A14; D030051N19Rik;
                     mKIAA1736"
                     /inference="alignment:Splign:2.1.0"
     exon            3481..3573
                     /gene="Ambra1"
                     /gene_synonym="2310079H06Rik; A130023A14; D030051N19Rik;
                     mKIAA1736"
                     /inference="alignment:Splign:2.1.0"
     exon            3574..3767
                     /gene="Ambra1"
                     /gene_synonym="2310079H06Rik; A130023A14; D030051N19Rik;
                     mKIAA1736"
                     /inference="alignment:Splign:2.1.0"
     exon            3768..5290
                     /gene="Ambra1"
                     /gene_synonym="2310079H06Rik; A130023A14; D030051N19Rik;
                     mKIAA1736"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5267..5272
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ambra1"
                     /gene_synonym="2310079H06Rik; A130023A14; D030051N19Rik;
                     mKIAA1736"
                     /note="hexamer: ATTAAA"
     polyA_site      5290
                     /gene="Ambra1"
                     /gene_synonym="2310079H06Rik; A130023A14; D030051N19Rik;
                     mKIAA1736"
                     /note="major polyA site"
ORIGIN      
        1 tttgatgtct gggcgttcgt gtgttcgatg atcttttgcg gcgtcttctt ggttgatgtc
       61 tccggagcgg cggcggctgt gccaggtcct ggactgagtt atcccggagg ccccgggctc
      121 tggccgcttt cttccgctgg tcggtcgagc ggacccctcg ctttcttcgg cctctccccg
      181 ccttccgtcc gtggctccgt gcgctcgctg gctttcgggc cgagctctca ctctcctcgc
      241 agatttccat gaggacagct gacaaggacc tgaatggcct cattttagct atttctgtgt
      301 gtggagctat tgcttgctcc ttcagttctg atccctggtg cgacagtggc tcctgagcac
      361 catgaaagtt gtcccagaga agaatgctgt acgaatcctc tggggccgag aacggggcac
      421 tcgtgccatg ggtgcccagc ggcttctgca ggagctggtg gaggataaga ctcgatggat
      481 gaaatgggag ggcaagagag tagaactgcc tgatagtcca cgctcgacct tcttactggc
      541 cttcagccca gacaggactc tcttggcatc cacacatgtg aaccataaca tctatattac
      601 agaggttaag actggcaagt gtgttcactc tctgatcgga caccgccgta ctccatggtg
      661 tgtcactttt caccccacca tctcaggcct tattgcttct ggttgcctag atggggaagt
      721 taggatttgg gatttacatg ggggcagtga aagctggttc acagacagca acaatgccat
      781 agcctccttg gctttccacc ctacggctca gcttctacta attgccactg caaatgagat
      841 ccatttctgg gattggagtc gtcgggagcc ctttgctgtg gtgaaaacag ccagtgagat
      901 ggagcgtgtc cgcctggtga gatttgatcc acttggacac tacttactta cagctattgt
      961 taacccttct aatcagcagg gtgatgacga accagaaatc cctatagatg gaacagagtt
     1021 atctcattac cgtcaacgtg ccctcctgca atcacagcca gttcgccgga cacctctcct
     1081 ccacaatttc ctgcacatgc tgtcctcccg ttcctctggc atccaggtgg gagagcaaag
     1141 cacagtacaa gattctgcta ccccctcacc cccaccgcct ccccctcagc cctccacgga
     1201 gcgtcccagg acttccgctt acatcagact ccgacagcgg gtcagttacc ccaccacagt
     1261 tgagtgctgc cagcaccctg ggatcctgtg cctttgcagc cgctgtgctg gcactcgagt
     1321 tccttccctc ttgccacacc aggacagtgt cccccctgct tctgccagag ctactacccc
     1381 ttccttttct tttgtacaga ccgagccctt ccatcccccg gagcaggctt catcaacgca
     1441 gcaggaccag ggcctcctga accggccgtc tgccttcagt acagtccaga gcagcactgc
     1501 gggcaacacg ctccgcaacc tcagtctggg tcctacccgg cgctctttgg ggggcccttt
     1561 gtctagccac ccttctaggt atcacagaga actagcccct gggctgacag gttctgagtg
     1621 gacccggaca gtactcactc tgaactctcg ttctgaggta gaatctatgc ccccaccgag
     1681 gaccagtgcc tcctcagtga gtttgctttc tgtactgaga cagcaggaag gtggctctca
     1741 ggcatctgtg tatacttcag ccacagaagg gaggggtttt ccatcatcag ggttggcaac
     1801 tgagtcagat ggagggaatg gttccagcca aaacaactca ggcagcattc gccatgagct
     1861 tcaatgtgac ctgagacgct tctttttgga gtatgacagg ctgcaggagc tggatcagag
     1921 cctgagtggg gaaactcccc agacccaaca ggcccaggaa atgctcaaca ataatattga
     1981 atctgagaga ccaggtcctt cccacctgcc taccccacac agcagtgaga acaactccaa
     2041 cctgtctcgg ggccacctta atcgatgccg tgcttgccat aatcttctaa ccttcaacaa
     2101 tgataccctt cgttgggaaa gaaccacacc taactactca tctggtgagg ctagctcttc
     2161 ctggcacgtc tccactacct ttgagggcat gccaccaagt ggcaaccagc taccacctct
     2221 tgagcggact gagggccaaa tgcctagctc cagcaggctg gagttgagca gctctgctag
     2281 ttcacaagag gagaggactg tgggggtggc ctttaaccag gagacaggcc actgggaacg
     2341 catttatacc cagtccagca gatctggaac tgtatcacag gaggccttac atcaagatat
     2401 gcctgaagaa agctctgagg aagactccct caggaggagg ctgctggagt cttccctcat
     2461 ttcattatcc cgttatgatg gagcaggatc cagagagcac ccaatttacc cagacccagc
     2521 gagattatct cctgctgcat actacgccca gaggatgatc cagtatctct caaggagaga
     2581 cagtattcgc cagcgctcca tgcgctacca acagaatcgg cttcgttctt ccacctcctc
     2641 ttcttcctca gacaaccagg gtccatcagt agagggaacc gacttggaat ttgaggactt
     2701 tgaggacaat ggtgacagat ctaggcaccg agctccccga aatgcccgaa tgtctgcacc
     2761 ttcacttgga cgatttgttc caaggcgttt tttgctgcct gagtacttgc cttatgctgg
     2821 gatttttcat gaacgtggac agcctggctt ggctactcat tcttctgtta acagggtcct
     2881 ggcaggggca gtaattggag atggacagtc tgctgtggcc agtaacattg ccaatactac
     2941 ctacaggctc cagtggtggg acttcactaa gtttgacctt ccagaaatca gtaatgcctc
     3001 tgtgaatgtc ctggtacaga attgcaagat ctacaatgat gccagctgtg acatttctgc
     3061 agatggtcag ctcctggcag ccttcatccc gagcagccag aggggctttc ctgatgaagg
     3121 catcctggca gtgtattccc tggcccccca taacttgggg gaaatgctct acaccaagcg
     3181 atttggtccc aatgccattt ctgtaagcct gtccccgatg ggccgatacg ttatggtggg
     3241 cttggcctca cgaaggatcc tactgcaccc ctccacagag cacatggtag cccaggtctt
     3301 caggctgcaa caggcccatg gtggtgagac ttccatgagg agagttttca atgtccttta
     3361 ccccatgcct gcagaccaac ggagacacgt cagcatcaac tctgcccgtt ggctacccga
     3421 gccagggctt ggtttggcct atggcaccaa caaaggagac ttggtgatct gccgaccaga
     3481 ggccttaaac tctggcatcg agtactactg ggaccagcta agtgaaacag tcttcactgt
     3541 ccactcaagt agcaggagca gcgagcggcc tggaaccagc agagccacgt ggcggacaga
     3601 cagagacatg ggtttgatga atgcaattgg gctgcagcct cggaacccaa ccacctctgt
     3661 gacatctcag ggcacccaga ctctggccct ccagttgcag aacgctgaaa cacagactga
     3721 gagggaggaa gaggaaccag gggcagcatc ttcaggtcct ggtgaaggcg agggctcaga
     3781 atatggtggc agtggggaag atgccctcag caggatccag aggctgatgg ccgagggtgg
     3841 catgacagct gtggtacaac gggaacagag caccaccatg gcctccatgg gtggctttgg
     3901 caacaacatc atcgttagcc accgcattca ccgcagctcc cagacaggca cagagtcagg
     3961 ggctgctcgt acctcctcac cccagccctc cacctctcga ggactgccat cagaacctgg
     4021 gcaactggca gagcgagcgc taagcccccg gacagcctcc tgggaccagc ctagtacctc
     4081 tgggagggaa ctaccccagc cagccctgtc ctcttcctcc cctgtcccca ttccagtccc
     4141 ccttgccagc aatgagggac caaccatgca ctgcaatgtg accaataaca gtcaccttcc
     4201 tgagggtgat ggtagcaaca ggggggaagc tgcaggccct agtggagagc cacagaaccg
     4261 atagagacaa aatgaggcac tggtacctcc ctcaagctgc tgagcagaag caggacctca
     4321 cagctgactg ggaactgtac acacagaaga gctgatgcct gcatccagga acaggaggaa
     4381 tgagagggtg gggcatggag ccagtggata agggggcagt ccaaggggca aggctggcct
     4441 ggggtactag agccttccat gatctagagc ccagaagaac cacactgctg agcctggtgt
     4501 gaatgaagaa gcaagtgggt gtctctgcta tgaaccccca tttctcctag gatcactggc
     4561 ctaggggact caaacaaccc tgggcagaag ccccagaaga gtaaagtctt ccagatttag
     4621 accacaccat cctggacaga atggtgggac agttctcccc tgcccactac tggagggaca
     4681 agccaccagg agcagaggag caacaccccc cccccattgc ttccatctct aaaccttcca
     4741 gagttccagc ctctctcatc ctcttagccc acagagatgg aaaagtcagg cacagcttcc
     4801 atgtcagtga cttatgctca ccaaatgcgg acacctgtgg agtgaaggaa atagcctgct
     4861 tgcctgacac agaatggagt caatacatgg tggtgaacag gtcctcagga gtgggggtgt
     4921 aggctggccc gcccccagca tgtccatcaa gctcccccct ccacaaggcc ctccatgcag
     4981 cacctatggg tgtcagtatt acctaagcat aggccaaagc cagcccaggg ctggagggag
     5041 gagattccag gtcagaatgt gaggtcttgg tctgtgattt gaggtgttgc atgtggaatc
     5101 ctgaactgta cgtgtagttt ttagtggcga aatcaaggct ctgatcattt tgtttttagt
     5161 atgaaaatgt gatttccttt ctgtttgtca ctcatcatag aaacattgtg gtgggaggag
     5221 aggggatagt ctactgctaa tgagggagac accaaagatc tacatcatta aaatgatgac
     5281 acgcccctca aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaa
//
