LOCUS       NM_009664               1874 bp    mRNA    linear   ROD 28-JAN-2025
DEFINITION  Mus musculus ameloblastin (Ambn), transcript variant 2, mRNA.
ACCESSION   NM_009664
VERSION     NM_009664.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1874)
  AUTHORS   Zhang,J., Gao,J., Zeng,X., Wang,Z., Chen,C., Rong,C., Li,S.,
            Cai,L., Wang,L., Zhang,L. and Tian,Z.
  TITLE     A novel Cdc42-YAP-fibronectin signaling axis regulates ameloblast
            differentiation during early enamel formation
  JOURNAL   Biochim Biophys Acta Mol Basis Dis 1871 (2), 167570 (2025)
   PUBMED   39547518
REFERENCE   2  (bases 1 to 1874)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 1874)
  AUTHORS   Gruper,Y., Wolff,A.S.B., Glanz,L., Spoutil,F., Marthinussen,M.C.,
            Osickova,A., Herzig,Y., Goldfarb,Y., Aranaz-Novaliches,G.,
            Dobes,J., Kadouri,N., Ben-Nun,O., Binyamin,A., Lavi,B., Givony,T.,
            Khalaila,R., Gome,T., Wald,T., Mrazkova,B., Sochen,C., Besnard,M.,
            Ben-Dor,S., Feldmesser,E., Orlova,E.M., Hegedus,C., Lampe,I.,
            Papp,T., Felszeghy,S., Sedlacek,R., Davidovich,E., Tal,N.,
            Shouval,D.S., Shamir,R., Guillonneau,C., Szondy,Z., Lundin,K.E.A.,
            Osicka,R., Prochazka,J., Husebye,E.S. and Abramson,J.
  TITLE     Autoimmune amelogenesis imperfecta in patients with APS-1 and
            coeliac disease
  JOURNAL   Nature 624 (7992), 653-662 (2023)
   PUBMED   37993717
REFERENCE   4  (bases 1 to 1874)
  AUTHORS   Nag,P., Inubushi,T., Sasaki,J.I., Murotani,T., Kusano,S.,
            Nakanishi,Y., Shiraishi,Y., Kurosaka,H., Imazato,S., Yamaguchi,Y.
            and Yamashiro,T.
  TITLE     Tmem2 Deficiency Leads to Enamel Hypoplasia and Soft Enamel in
            Mouse
  JOURNAL   J Dent Res 102 (10), 1162-1171 (2023)
   PUBMED   37449307
REFERENCE   5  (bases 1 to 1874)
  AUTHORS   Mitsiadis,T.A., Pagella,P., Gomes Rodrigues,H., Tsouknidas,A.,
            Ramenzoni,L.L., Radtke,F., Mehl,A. and Viriot,L.
  TITLE     Notch Signaling Pathway in Tooth Shape Variations throughout
            Evolution
  JOURNAL   Cells 12 (5), 761 (2023)
   PUBMED   36899896
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1874)
  AUTHORS   Bouwman,P., Gollner,H., Elsasser,H.P., Eckhoff,G., Karis,A.,
            Grosveld,F., Philipsen,S. and Suske,G.
  TITLE     Transcription factor Sp3 is essential for post-natal survival and
            late tooth development
  JOURNAL   EMBO J 19 (4), 655-661 (2000)
   PUBMED   10675334
REFERENCE   7  (bases 1 to 1874)
  AUTHORS   Begue-Kirn,C., Krebsbach,P.H., Bartlett,J.D. and Butler,W.T.
  TITLE     Dentin sialoprotein, dentin phosphoprotein, enamelysin and
            ameloblastin: tooth-specific molecules that are distinctively
            expressed during murine dental differentiation
  JOURNAL   Eur J Oral Sci 106 (5), 963-970 (1998)
   PUBMED   9786327
REFERENCE   8  (bases 1 to 1874)
  AUTHORS   Rajan,L., Kozak,C.A. and Dudley,J.P.
  TITLE     Chromosomal localization of acquired MMTV proviral integration
            sites in T-cell lymphomas
  JOURNAL   Mamm Genome 9 (1), 84-85 (1998)
   PUBMED   9434954
REFERENCE   9  (bases 1 to 1874)
  AUTHORS   MacDougall,M., DuPont,B.R., Simmons,D., Reus,B., Krebsbach,P.,
            Karrman,C., Holmgren,G., Leach,R.J. and Forsman,K.
  TITLE     Ameloblastin gene (AMBN) maps within the critical region for
            autosomal dominant amelogenesis imperfecta at chromosome 4q21
  JOURNAL   Genomics 41 (1), 115-118 (1997)
   PUBMED   9126491
REFERENCE   10 (bases 1 to 1874)
  AUTHORS   Krebsbach,P.H., Lee,S.K., Matsuki,Y., Kozak,C.A., Yamada,K.M. and
            Yamada,Y.
  TITLE     Full-length sequence, localization, and chromosomal mapping of
            ameloblastin. A novel tooth-specific gene
  JOURNAL   J Biol Chem 271 (8), 4431-4435 (1996)
   PUBMED   8626794
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC087927.1 and AC121585.3.
            
            On Jan 1, 2015 this sequence version replaced NM_009664.1.
            
            Summary: This gene encodes an extracellular matrix glycoprotein
            that is involved in the formation of dental enamel. Mice lacking
            the encoded protein fail to undergo normal ameloblast
            differentiation and develop enamel. Mice overproducing the product
            of this gene develop thinner and more porous enamel, with disrupted
            rod patterns and abnormal crystallites. Alternate splicing of this
            gene results in multiple transcript variants. [provided by RefSeq,
            Dec 2014].
            
            Transcript Variant: This variant (2) uses an alternate in-frame
            splice site in the coding region, compared to variant 1. This
            results in a shorter protein (isoform 2), compared to isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: U65021.1, AK017250.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164137, SAMN04095718
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-20                BC087927.1         1-20
            21-116              AC121585.3         118353-118448
            117-185             AC121585.3         119227-119295
            186-251             AC121585.3         121701-121766
            252-299             AC121585.3         123045-123092
            300-410             AC121585.3         123975-124085
            411-605             AC121585.3         125614-125808
            606-644             AC121585.3         126813-126851
            645-704             AC121585.3         127473-127532
            705-749             AC121585.3         127632-127676
            750-794             AC121585.3         128048-128092
            795-1874            AC121585.3         129794-130873
FEATURES             Location/Qualifiers
     source          1..1874
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 43.63 cM"
     gene            1..1874
                     /gene="Ambn"
                     /note="ameloblastin"
                     /db_xref="GeneID:11698"
                     /db_xref="MGI:MGI:104655"
     exon            1..116
                     /gene="Ambn"
                     /inference="alignment:Splign:2.1.0"
     CDS             102..1325
                     /gene="Ambn"
                     /note="isoform 2 precursor is encoded by transcript
                     variant 2"
                     /codon_start=1
                     /product="ameloblastin isoform 2 precursor"
                     /protein_id="NP_033794.1"
                     /db_xref="CCDS:CCDS51539.1"
                     /db_xref="GeneID:11698"
                     /db_xref="MGI:MGI:104655"
                     /translation="MSASKIPLFKMKGLILFLSLVKMSLAVPAFPQQPGAQGMAPPGM
                     ASLSLETMRQLGSLQGLNALSQYSRLGFGKALNSLWLHGLLPPHNSFPWIGPREHETQ
                     QPSLQPHQPGLKPFLQPTAATGVQVTPQKPGPQPPMHPGQLPLQEGELIAPDEPQVAP
                     SENPPTPEVPIMDFADPQFPTVFQIARSISRGPMAHNKASAFYPGMFYMSYGANQLNA
                     PARIGFMSSEEMPGERGSPMAYGTLFPRFGGFRQTLRRLNQNSPKGGDFTVEVDSPVS
                     VTKGPEKGEGPEGSPLQEANPGKRENPALLSQMAPGAHAGLLAFPNDHIPSMARGPAG
                     QRLLGVTPAAADPLITPELAEVYETYGADVTTPLGDGEATMDITMSPDTQQPLLPGNK
                     VHQPQVHNAWRFQEP"
     exon            117..185
                     /gene="Ambn"
                     /inference="alignment:Splign:2.1.0"
     exon            186..251
                     /gene="Ambn"
                     /inference="alignment:Splign:2.1.0"
     exon            252..299
                     /gene="Ambn"
                     /inference="alignment:Splign:2.1.0"
     exon            300..410
                     /gene="Ambn"
                     /inference="alignment:Splign:2.1.0"
     exon            411..605
                     /gene="Ambn"
                     /inference="alignment:Splign:2.1.0"
     exon            606..644
                     /gene="Ambn"
                     /inference="alignment:Splign:2.1.0"
     exon            645..704
                     /gene="Ambn"
                     /inference="alignment:Splign:2.1.0"
     exon            705..749
                     /gene="Ambn"
                     /inference="alignment:Splign:2.1.0"
     exon            750..794
                     /gene="Ambn"
                     /inference="alignment:Splign:2.1.0"
     exon            795..1874
                     /gene="Ambn"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1508..1513
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ambn"
                     /note="hexamer: AATAAA"
     polyA_site      1527
                     /gene="Ambn"
                     /note="major polyA site"
     regulatory      1850..1855
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ambn"
                     /note="hexamer: AATAAA"
     polyA_site      1874
                     /gene="Ambn"
                     /note="major polyA site"
ORIGIN      
        1 gagagataca gagagagccc caggaacagt ccagaaaaaa aattaatctt cttttcttag
       61 aactgttttg attggcatca tcaggcctgg gagcacagtg aatgtcagca tctaagattc
      121 cacttttcaa aatgaagggc ctgatcctgt tcctgtccct agtgaaaatg agcctcgccg
      181 tgccggcatt tcctcaacaa cctggggctc aaggcatggc acctcctggc atggctagtt
      241 tgagccttga gacaatgaga cagttgggaa gcttgcaggg actcaacgca ctttctcagt
      301 attctagact tggctttgga aaagcactta atagtttatg gttgcacgga cttctcccac
      361 cgcataactc tttcccatgg ataggaccaa gggaacatga aacccagcag ccatccttgc
      421 agcctcacca gccaggactg aaacccttcc tccagcccac tgctgcaacc ggtgtccagg
      481 tcacacccca gaagccaggg cctcagcctc caatgcaccc tggacagctg cccttgcagg
      541 aaggagagct gatagcacca gatgagccgc aggtggcacc atccgaaaac ccaccaacac
      601 ctgaggtacc aataatggat tttgctgatc cacaatttcc aaccgtgttc cagatcgccc
      661 gttcaatatc tcggggacca atggcacaca acaaagcatc cgctttttac ccaggaatgt
      721 tttacatgtc ttatggagca aaccaattga atgctcctgc cagaattggc ttcatgagtt
      781 cagaagaaat gcctggagaa agaggaagtc ccatggccta tggaactctg ttcccaagat
      841 ttggaggctt caggcaaacc cttaggagac tgaatcagaa ttcacccaag ggaggagact
      901 ttactgtgga agtagattcc ccagtatctg ttaccaaagg ccctgaaaaa ggagaaggtc
      961 cagaaggctc tccactgcaa gaggccaacc caggcaaacg ggaaaacccc gctctccttt
     1021 cacaaatggc acctggggcc catgcaggac ttcttgcttt ccccaatgac cacatcccca
     1081 gtatggcaag gggtcctgca gggcaaagac tccttggagt cacccctgca gctgcagacc
     1141 cactgatcac ccctgaatta gcagaagttt atgaaaccta tggtgctgat gttaccacac
     1201 ccctgggtga tggagaagca accatggata tcaccatgtc cccagacact cagcagccac
     1261 tgctacctgg aaacaaagtg caccagcccc aggtgcacaa cgcatggcgt ttccaagagc
     1321 cctgataacc ttgacatagc agctactttg tgtatgcaca agctttccag ctttgtcccc
     1381 atagtacctt gttgctaaaa cacttactat ccttccgcag tgaaggtgtt aagagcacta
     1441 agcatatatt aataaataca agtggctaga aatagtgtag gtcccttctt gcttccattc
     1501 ttatcaaaat aaaatgtatc aactgtctcc gtgacttaga aatactatca atgatatcag
     1561 agcaagtcta agtgtcagca cttggtggta tagcatgtag ctgtcttagg cattataaaa
     1621 tttctcttac tacatgacat tattatgccc aggaaatgtg acactgcttc tttctctaca
     1681 caaaagcact tagtttcaga attccaaagt atttcattta aaccttatta aatggtgatt
     1741 ggtggaaaac cctgactgct attactggtt tcatatattg gatttaaaat tcttatttat
     1801 agaatatttt atttaatcta ggaaaagaaa gggcaattgg cctgttttaa ataaagaatt
     1861 tttctcactg aaaa
//
