LOCUS       NM_008537               1525 bp    mRNA    linear   ROD 13-JUN-2024
DEFINITION  Mus musculus alpha-methylacyl-CoA racemase (Amacr), transcript
            variant 1, mRNA.
ACCESSION   NM_008537
VERSION     NM_008537.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Tafferner,N., Barthelmes,J., Eberle,M., Ulshofer,T., Henke,M.,
            deBruin,N., Mayer,C.A., Foerch,C., Geisslinger,G., Parnham,M.J. and
            Schiffmann,S.
  TITLE     Alpha-methylacyl-CoA racemase deletion has mutually counteracting
            effects on T-cell responses, associated with unchanged course of
            EAE
  JOURNAL   Eur J Immunol 46 (3), 570-581 (2016)
   PUBMED   26648339
  REMARK    GeneRIF: observed a disease-dependent elevation of AMACR expression
            in monocytes and T cells from blood, draining lymph nodes and
            spleen of autoimmune experimental encephalomyelitis mice during
            preclinical phase; in vitro analysis revealed proliferation of T
            cells was inhibited in AMACR KO mice, but T-cell polarization was
            switched toward a pathogenic state
REFERENCE   2  (bases 1 to 1525)
  AUTHORS   Selkala,E.M., Nair,R.R., Schmitz,W., Kvist,A.P., Baes,M.,
            Hiltunen,J.K. and Autio,K.J.
  TITLE     Phytol is lethal for Amacr-deficient mice
  JOURNAL   Biochim Biophys Acta 1851 (10), 1394-1405 (2015)
   PUBMED   26248199
  REMARK    GeneRIF: Phytol causes primary failure in liver leading to death of
            Amacr-/- mice thus emphasizing the indispensable role of Amacr in
            detoxification of alpha-methyl-branched fatty acids.
REFERENCE   3  (bases 1 to 1525)
  AUTHORS   Autio,K.J., Schmitz,W., Nair,R.R., Selkala,E.M., Sormunen,R.T.,
            Miinalainen,I.J., Crick,P.J., Wang,Y., Griffiths,W.J., Reddy,J.K.,
            Baes,M. and Hiltunen,J.K.
  TITLE     Role of AMACR (alpha-methylacyl-CoA racemase) and MFE-1
            (peroxisomal multifunctional enzyme-1) in bile acid synthesis in
            mice
  JOURNAL   Biochem J 461 (1), 125-135 (2014)
   PUBMED   24735479
  REMARK    GeneRIF: AMACR has a role in the synthesis of bile acids.
REFERENCE   4  (bases 1 to 1525)
  AUTHORS   Selkala,E.M., Kuusisto,S.M., Salonurmi,T., Savolainen,M.J.,
            Jauhiainen,M., Pirila,P.L., Kvist,A.P., Conzelmann,E., Schmitz,W.,
            Alexson,S.E., Kotti,T.J., Hiltunen,J.K. and Autio,K.J.
  TITLE     Metabolic adaptation allows Amacr-deficient mice to remain
            symptom-free despite low levels of mature bile acids
  JOURNAL   Biochim Biophys Acta 1831 (8), 1335-1343 (2013)
   PUBMED   23680781
  REMARK    GeneRIF: Metabolic adaptation allows Amacr-deficient mice to remain
            symptom-free despite low levels of mature bile acids.
REFERENCE   5  (bases 1 to 1525)
  AUTHORS   Savolainen,K., Kotti,T.J., Schmitz,W., Savolainen,T.I.,
            Sormunen,R.T., Ilves,M., Vainio,S.J., Conzelmann,E. and
            Hiltunen,J.K.
  TITLE     A mouse model for alpha-methylacyl-CoA racemase deficiency:
            adjustment of bile acid synthesis and intolerance to dietary
            methyl-branched lipids
  JOURNAL   Hum Mol Genet 13 (9), 955-965 (2004)
   PUBMED   15016763
REFERENCE   6  (bases 1 to 1525)
  AUTHORS   Amery,L., Fransen,M., De Nys,K., Mannaerts,G.P. and Van
            Veldhoven,P.P.
  TITLE     Mitochondrial and peroxisomal targeting of 2-methylacyl-CoA
            racemase in humans
  JOURNAL   J Lipid Res 41 (11), 1752-1759 (2000)
   PUBMED   11060344
REFERENCE   7  (bases 1 to 1525)
  AUTHORS   Kotti,T.J., Savolainen,K., Helander,H.M., Yagi,A., Novikov,D.K.,
            Kalkkinen,N., Conzelmann,E., Hiltunen,J.K. and Schmitz,W.
  TITLE     In mouse alpha -methylacyl-CoA racemase, the same gene product is
            simultaneously located in mitochondria and peroxisomes
  JOURNAL   J Biol Chem 275 (27), 20887-20895 (2000)
   PUBMED   10770938
REFERENCE   8  (bases 1 to 1525)
  AUTHORS   Ferdinandusse,S., Denis,S., Clayton,P.T., Graham,A., Rees,J.E.,
            Allen,J.T., McLean,B.N., Brown,A.Y., Vreken,P., Waterham,H.R. and
            Wanders,R.J.
  TITLE     Mutations in the gene encoding peroxisomal alpha-methylacyl-CoA
            racemase cause adult-onset sensory motor neuropathy
  JOURNAL   Nat Genet 24 (2), 188-191 (2000)
   PUBMED   10655068
REFERENCE   9  (bases 1 to 1525)
  AUTHORS   Schmitz,W., Helander,H.M., Hiltunen,J.K. and Conzelmann,E.
  TITLE     Molecular cloning of cDNA species for rat and mouse liver
            alpha-methylacyl-CoA racemases
  JOURNAL   Biochem J 326 (Pt 3) (Pt 3), 883-889 (1997)
   PUBMED   9307041
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC102219.11.
            
            On Aug 24, 2022 this sequence version replaced NM_008537.4.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK002401.1, AK048249.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on single
                                                  protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-265               AC102219.11        83299-83563
            266-409             AC102219.11        84860-85003
            410-570             AC102219.11        86196-86356
            571-757             AC102219.11        90269-90455
            758-1525            AC102219.11        96350-97117
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="15"
                     /map="15 5.39 cM"
     gene            1..1525
                     /gene="Amacr"
                     /gene_synonym="Macr1"
                     /note="alpha-methylacyl-CoA racemase"
                     /db_xref="GeneID:17117"
                     /db_xref="MGI:MGI:1098273"
     exon            1..265
                     /gene="Amacr"
                     /gene_synonym="Macr1"
                     /inference="alignment:Splign:2.1.0"
     CDS             22..1167
                     /gene="Amacr"
                     /gene_synonym="Macr1"
                     /EC_number="5.1.99.4"
                     /note="2-methylacyl-CoA racemase;
                     alpha-methylacyl-Coenzyme A racemase; 2-arylpropionyl-CoA
                     epimerase"
                     /codon_start=1
                     /product="alpha-methylacyl-CoA racemase"
                     /protein_id="NP_032563.2"
                     /db_xref="CCDS:CCDS27381.1"
                     /db_xref="GeneID:17117"
                     /db_xref="MGI:MGI:1098273"
                     /translation="MVLRGVRVVELAGLAPGPFCGMVLADFGAEVVRVNRLGSTGENF
                     LARGKRSLALDLKRSQGVTVLRRMCARADVLLEPFRCGVMEKLQLGPETLLQDNPKLI
                     YARLSGFGQSGIFSKVAGHDINYLALSGVLSKIGRSGENPYPPLNLLADFGGGGLMCT
                     LGIVLALFERTRSGRGQVIDSSMVEGTAYLSSFLWKTQPMGLWKQPRGQNILDGGAPF
                     YTTYKTADGEFMAVGAIEPQFYALLLKGLGLESEELPSQMSSADWPEMKKKFADVFAK
                     KTKAEWCQIFDGTDACVTPVLTFEEALHHQHNRERASFITDGEQLPSPRPAPLLSRTP
                     AVPSAKRDPSVGEHTVEVLREYGFSQEEILQLHSDRIVESDKLKANL"
     exon            266..409
                     /gene="Amacr"
                     /gene_synonym="Macr1"
                     /inference="alignment:Splign:2.1.0"
     exon            410..570
                     /gene="Amacr"
                     /gene_synonym="Macr1"
                     /inference="alignment:Splign:2.1.0"
     exon            571..757
                     /gene="Amacr"
                     /gene_synonym="Macr1"
                     /inference="alignment:Splign:2.1.0"
     exon            758..1525
                     /gene="Amacr"
                     /gene_synonym="Macr1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1476..1481
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Amacr"
                     /gene_synonym="Macr1"
                     /note="hexamer: AATAAA"
     polyA_site      1496
                     /gene="Amacr"
                     /gene_synonym="Macr1"
                     /note="major polyA site"
     regulatory      1499..1504
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Amacr"
                     /gene_synonym="Macr1"
                     /note="hexamer: AATAAA"
     polyA_site      1525
                     /gene="Amacr"
                     /gene_synonym="Macr1"
                     /note="major polyA site"
ORIGIN      
        1 gctctcaccc taaggctggc catggtgctg cgtggcgtca gggttgtgga gctggcaggc
       61 ctggccccgg ggccgttctg cggaatggtc ctggcggact tcggcgccga ggtggtgcgc
      121 gtgaaccggc tgggctccac gggcgagaat tttctggccc gaggcaagcg ctcgctagcg
      181 ctggacctga agcgctctca gggagtcacg gtgttgcggc gcatgtgcgc acgcgcggac
      241 gtgttgctgg agcccttccg ctgcggtgtc atggagaaac tccagcttgg gccagagact
      301 ctactgcagg acaatccaaa gctcatctat gccaggctga gcggatttgg ccaatcggga
      361 attttctcca aagtagctgg ccatgacatc aactatttgg ctttatcagg cgttctgtca
      421 aagattggca gaagcggtga gaacccctac ccaccgctga atctcctggc tgactttggc
      481 ggtggaggcc tcatgtgcac actgggcatt gtgctggctc tctttgaacg cacacgctct
      541 ggccgagggc aggtcatcga ttcaagcatg gtggaaggga ctgcatactt aagttctttc
      601 ctgtggaaaa cccagcccat gggtctgtgg aaacagcctc gaggacaaaa catcttagat
      661 ggcggtgcac ctttctacac aacctacaag acggcagacg gggagttcat ggctgtaggt
      721 gccatagaac cccagttcta tgcactgctg cttaaaggac ttggactcga gtctgaggaa
      781 ctcccctccc agatgagctc agcagattgg ccagagatga agaagaaatt tgcagatgtg
      841 tttgcaaaga agactaaggc agaatggtgc cagatctttg acgggacaga tgcgtgtgtg
      901 accccagtgc tgacgtttga ggaggccctc caccaccagc acaacagaga acgggcctcc
      961 ttcatcactg atggggagca gctcccgagc ccccgccctg cacctctgct ttccagaact
     1021 cctgccgtcc catctgccaa aagggaccct tctgtagggg agcacaccgt agaagtgctt
     1081 agagagtatg gattcagtca ggaagagatc cttcagctgc actcagatag aatcgttgaa
     1141 agtgataagc taaaagccaa tctctgactc aggcttatag ctcaagagaa tctgaaggct
     1201 gcatctccac tggggaggga tgcccacaat tgtgtgtatg gaaatgtgga tgaacagcaa
     1261 tgaagtcatc caaatatccc aatcacgatc caacgaaagg ctggttacag gataacgatt
     1321 gcgcccttac gctgcttatc agagcctctg attgaggaaa atttgtgtgt gtgtactgat
     1381 agtaacttgt ggcagctttc tgcctttcag ttcccttggt gaagtgtatc catttattaa
     1441 aaccctttta taaatacaac tctgatcata tattaaataa actattataa ctttaataaa
     1501 taaaactctt gttttcctcc tcaaa
//
