LOCUS       NM_028717               6512 bp    mRNA    linear   ROD 04-JUN-2024
DEFINITION  Mus musculus alsin Rho guanine nucleotide exchange factor (Als2),
            transcript variant 1, mRNA.
ACCESSION   NM_028717
VERSION     NM_028717.6
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 6512)
  AUTHORS   Sato,K., Suzuki-Utsunomiya,K., Mitsui,S., Ono,S., Shimakura,K.,
            Otomo,A. and Hadano,S.
  TITLE     Central nervous system specific high molecular weight ALS2/alsin
            homophilic complex is enriched in mouse brain synaptosomes
  JOURNAL   Biochem Biophys Res Commun 638, 168-175 (2023)
   PUBMED   36459881
REFERENCE   2  (bases 1 to 6512)
  AUTHORS   Rivas,S., Silva,P., Reyes,M., Sepulveda,H., Solano,L., Acuna,J.,
            Guerrero,M., Varas-Godoy,M., Quest,A.F.G., Montecino,M. and
            Torres,V.A.
  TITLE     The RabGEF ALS2 is a hypoxia inducible target associated with the
            acquisition of aggressive traits in tumor cells
  JOURNAL   Sci Rep 10 (1), 22302 (2020)
   PUBMED   33339852
  REMARK    GeneRIF: The RabGEF ALS2 is a hypoxia inducible target associated
            with the acquisition of aggressive traits in tumor cells.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 6512)
  AUTHORS   Hadano,S., Mitsui,S., Pan,L., Otomo,A., Kubo,M., Sato,K., Ono,S.,
            Onodera,W., Abe,K., Chen,X., Koike,M., Uchiyama,Y., Aoki,M.,
            Warabi,E., Yamamoto,M., Ishii,T., Yanagawa,T., Shang,H.F. and
            Yoshii,F.
  TITLE     Functional links between SQSTM1 and ALS2 in the pathogenesis of
            ALS: cumulative impact on the protection against mutant
            SOD1-mediated motor dysfunction in mice
  JOURNAL   Hum Mol Genet 25 (15), 3321-3340 (2016)
   PUBMED   27439389
  REMARK    GeneRIF: two ALS-linked factors, SQSTM1 and ALS2, have distinct but
            additive protective roles against mutant SOD1-mediated toxicity by
            modulating neuronal proteostasis possibly through the
            autophagy-endolysosomal system.
REFERENCE   4  (bases 1 to 6512)
  AUTHORS   Gautam,M., Jara,J.H., Sekerkova,G., Yasvoina,M.V., Martina,M. and
            Ozdinler,P.H.
  TITLE     Absence of alsin function leads to corticospinal motor neuron
            vulnerability via novel disease mechanisms
  JOURNAL   Hum Mol Genet 25 (6), 1074-1087 (2016)
   PUBMED   26755825
  REMARK    GeneRIF: By taking advantage of this CSMN reporter line of AlsinKO,
            authors reveal that apical dendrite degeneration is a common
            cellular pathology for CSMN that become diseased for different
            causes.
REFERENCE   5  (bases 1 to 6512)
  AUTHORS   Cobanoglu,G., Ozansoy,M. and Basak,A.N.
  TITLE     Are alsin and spartin novel interaction partners?
  JOURNAL   Biochem Biophys Res Commun 427 (1), 1-4 (2012)
   PUBMED   22982304
  REMARK    GeneRIF: alsin and spartin may interact each other physically.
REFERENCE   6  (bases 1 to 6512)
  AUTHORS   Nadeau,J.H.
  TITLE     Modifier genes and protective alleles in humans and mice
  JOURNAL   Curr Opin Genet Dev 13 (3), 290-295 (2003)
   PUBMED   12787792
  REMARK    Review article
REFERENCE   7  (bases 1 to 6512)
  AUTHORS   Okazaki,N., Kikuno,R., Ohara,R., Inamoto,S., Aizawa,H., Yuasa,S.,
            Nakajima,D., Nagase,T., Ohara,O. and Koga,H.
  TITLE     Prediction of the coding sequences of mouse homologues of KIAA
            gene: II. The complete nucleotide sequences of 400 mouse
            KIAA-homologous cDNAs identified by screening of terminal sequences
            of cDNA clones randomly sampled from size-fractionated libraries
  JOURNAL   DNA Res 10 (1), 35-48 (2003)
   PUBMED   12693553
REFERENCE   8  (bases 1 to 6512)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   9  (bases 1 to 6512)
  AUTHORS   Hadano,S., Hand,C.K., Osuga,H., Yanagisawa,Y., Otomo,A.,
            Devon,R.S., Miyamoto,N., Showguchi-Miyata,J., Okada,Y.,
            Singaraja,R., Figlewicz,D.A., Kwiatkowski,T., Hosler,B.A.,
            Sagie,T., Skaug,J., Nasir,J., Brown,R.H. Jr., Scherer,S.W.,
            Rouleau,G.A., Hayden,M.R. and Ikeda,J.E.
  TITLE     A gene encoding a putative GTPase regulator is mutated in familial
            amyotrophic lateral sclerosis 2
  JOURNAL   Nat Genet 29 (2), 166-173 (2001)
   PUBMED   11586298
REFERENCE   10 (bases 1 to 6512)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC153652.3, BC046828.1 and AB053307.1.
            
            On May 19, 2009 this sequence version replaced NM_028717.5.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC046828.1, AB053307.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-39                AC153652.3         31209-31247
            40-2424             BC046828.1         1-2385
            2425-3008           AB053307.1         2413-2996
            3009-6338           BC046828.1         2970-6299
            6339-6512           AC153652.3         105511-105684
FEATURES             Location/Qualifiers
     source          1..6512
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 29.33 cM"
     gene            1..6512
                     /gene="Als2"
                     /gene_synonym="3222402C23Rik; 9430073A21Rik; Als2cr6;
                     Alsin; mKIAA1563"
                     /note="alsin Rho guanine nucleotide exchange factor"
                     /db_xref="GeneID:74018"
                     /db_xref="MGI:MGI:1921268"
     exon            1..77
                     /gene="Als2"
                     /gene_synonym="3222402C23Rik; 9430073A21Rik; Als2cr6;
                     Alsin; mKIAA1563"
                     /inference="alignment:Splign:2.1.0"
     exon            78..155
                     /gene="Als2"
                     /gene_synonym="3222402C23Rik; 9430073A21Rik; Als2cr6;
                     Alsin; mKIAA1563"
                     /inference="alignment:Splign:2.1.0"
     CDS             136..5091
                     /gene="Als2"
                     /gene_synonym="3222402C23Rik; 9430073A21Rik; Als2cr6;
                     Alsin; mKIAA1563"
                     /note="isoform 1 is encoded by transcript variant 1;
                     amyotrophic lateral sclerosis protein 2 homolog;
                     amyotrophic lateral sclerosis 2 protein homolog;
                     amyotrophic lateral sclerosis 2 (juvenile) homolog"
                     /codon_start=1
                     /product="alsin isoform 1"
                     /protein_id="NP_082993.4"
                     /db_xref="CCDS:CCDS35583.1"
                     /db_xref="GeneID:74018"
                     /db_xref="MGI:MGI:1921268"
                     /translation="MDSKKKSSTEAEGSKERGLVHVWQAGSFSLTPERLPGWGGKTVL
                     QAALGVRHGVLLTEDGEVYSFGTLPWKSESAEICPSSPLLESALVGHHVITVATGSFH
                     SGAVTESGVVYMWGENAAGQCAVANQQYVPEPSPVSISDSETSPSLAVRILQLACGEE
                     HTLALSLSREIWAWGTGCQLGLITTTFPVTKPQKVEHLAGRVVLQVACGAFHSLALVQ
                     CLPPQDLKPVPERCNQCSQLLITMTDKEDHVIISDSHCCPLGVTLSESQAEKHASPAP
                     SPHPEALDEQGEVFENTVVEAELNMGSSQTTSGSAISTQQNVVGTAEVSSARTAPSYP
                     DTHAVTAYLQKLSEHSMRENHEPGEKPPQVQPLVEEAVPDLHSPPTTSTSALNSLVVS
                     CASAVGVRVAATYEAGALSLKKVMNFYSTAPCETAAQSGSASTGPESLKDLREEQVKQ
                     ESLQGKKSSSLMDIREEELEGGSRRLSLPGLLSQVSPRLLRKAARVKTRTVVLTPTYS
                     GEADALLPSLRTEVWTWGKGKEGQLGHGDVLPRLQPLCVKCLDGKEVIHLEAGGSHSL
                     ALTAKSQVYSWGSNTFGQLGHSEFPTTVPRLSKVSSENGVWSVAAGQDYSLFLVDTED
                     FQPGLYYSGRQDRAEGDTLPENPSGTKTPVLLSCSKLGYISRVTAGKDSYLALVDKNI
                     MGYIASLHELASTERRFYSKLSEIKSQILRPLLSLENLGTVTTVQLLQEVASRFSKLC
                     YLIGQHGASLSSYLQGMKEASSLVIMKHSSLFLDSYTEYCTSVSNFLVMGGFQLLAKP
                     AIDFLNKNQELLQDLSEVNDENTQLMEILNMLFFLPIRRLHNYAKVLLKLATCFEVTS
                     PEYQKLQDSSSCYESLALHLGKKRKEAEYTLSFWKTFPGKMTDSLRKPERRLLCESSN
                     RALSLQHAGRFSVNWFILFNDALVHAQFSTHHVFPLATLWAEPLSEEAGSVNGLKITT
                     PEEQFTLISSTPQEKTKWLRAISQAVDQALRGTSDFPLYGGGSSVQRQEPPISRSAKY
                     TFYKDTRLKDATYDGRWLSGKPHGRGVLKWPDGKMYSGMFRNGLEDGYGEYRIPNKAL
                     NKEDHYVGHWKEGKMCGQGVYSYASGEVFEGCFQDNMRHGHGLLRSGKLTSSSPSMFI
                     GQWVMDKKAGYGVFDDITRGEKYMGMWQDDVCQGNGVVVTQFGLYYEGNFHLNKMMGN
                     GVLLSEDDTIYEGEFSDDWTLSGKGTLTMPHGDYIEGYFSGEWGSGIKITGTYFKPSL
                     YESDKDKPKAFRKLGNLAVAADEKWRAVFEECWRQLGCESPGQGEVWKAWDNIAVALT
                     TNRRQHKDSPEILSRSQTQTLESLEYIPQHIGAFSVEKYDDIKKYLIKACDTPLHPLG
                     RLVETLVAVYRMTYVGVGANRRLLQEAVKEIKSYLKRIFQLVRFLFPELPEEGSTIPL
                     SAPLPTGRRSFCTGKSDSRSESPEPGYVVTSSGLLLPVLLPRLYPPLFMLYALDNDRE
                     EDIYWECVLRLNKQPDIALLGFLGVQKKFWPATLSILGESKKVLSTTKDACFASAVEC
                     LQQISTTFTPSDKLKVIQQTFEEISQSVLASLQEDFLWSMDDLFPVFLYVVLRARIRN
                     LGSEVHLIEDLMDPFLQHGEQGIMFTTLKACYFQIQREKLN"
     exon            156..310
                     /gene="Als2"
                     /gene_synonym="3222402C23Rik; 9430073A21Rik; Als2cr6;
                     Alsin; mKIAA1563"
                     /inference="alignment:Splign:2.1.0"
     exon            311..1230
                     /gene="Als2"
                     /gene_synonym="3222402C23Rik; 9430073A21Rik; Als2cr6;
                     Alsin; mKIAA1563"
                     /inference="alignment:Splign:2.1.0"
     exon            1231..1588
                     /gene="Als2"
                     /gene_synonym="3222402C23Rik; 9430073A21Rik; Als2cr6;
                     Alsin; mKIAA1563"
                     /inference="alignment:Splign:2.1.0"
     exon            1589..1757
                     /gene="Als2"
                     /gene_synonym="3222402C23Rik; 9430073A21Rik; Als2cr6;
                     Alsin; mKIAA1563"
                     /inference="alignment:Splign:2.1.0"
     exon            1758..1854
                     /gene="Als2"
                     /gene_synonym="3222402C23Rik; 9430073A21Rik; Als2cr6;
                     Alsin; mKIAA1563"
                     /inference="alignment:Splign:2.1.0"
     exon            1855..1932
                     /gene="Als2"
                     /gene_synonym="3222402C23Rik; 9430073A21Rik; Als2cr6;
                     Alsin; mKIAA1563"
                     /inference="alignment:Splign:2.1.0"
     exon            1933..2115
                     /gene="Als2"
                     /gene_synonym="3222402C23Rik; 9430073A21Rik; Als2cr6;
                     Alsin; mKIAA1563"
                     /inference="alignment:Splign:2.1.0"
     exon            2116..2287
                     /gene="Als2"
                     /gene_synonym="3222402C23Rik; 9430073A21Rik; Als2cr6;
                     Alsin; mKIAA1563"
                     /inference="alignment:Splign:2.1.0"
     exon            2288..2468
                     /gene="Als2"
                     /gene_synonym="3222402C23Rik; 9430073A21Rik; Als2cr6;
                     Alsin; mKIAA1563"
                     /inference="alignment:Splign:2.1.0"
     exon            2469..2534
                     /gene="Als2"
                     /gene_synonym="3222402C23Rik; 9430073A21Rik; Als2cr6;
                     Alsin; mKIAA1563"
                     /inference="alignment:Splign:2.1.0"
     exon            2535..2697
                     /gene="Als2"
                     /gene_synonym="3222402C23Rik; 9430073A21Rik; Als2cr6;
                     Alsin; mKIAA1563"
                     /inference="alignment:Splign:2.1.0"
     exon            2698..2829
                     /gene="Als2"
                     /gene_synonym="3222402C23Rik; 9430073A21Rik; Als2cr6;
                     Alsin; mKIAA1563"
                     /inference="alignment:Splign:2.1.0"
     exon            2830..2958
                     /gene="Als2"
                     /gene_synonym="3222402C23Rik; 9430073A21Rik; Als2cr6;
                     Alsin; mKIAA1563"
                     /inference="alignment:Splign:2.1.0"
     exon            2959..3029
                     /gene="Als2"
                     /gene_synonym="3222402C23Rik; 9430073A21Rik; Als2cr6;
                     Alsin; mKIAA1563"
                     /inference="alignment:Splign:2.1.0"
     exon            3030..3096
                     /gene="Als2"
                     /gene_synonym="3222402C23Rik; 9430073A21Rik; Als2cr6;
                     Alsin; mKIAA1563"
                     /inference="alignment:Splign:2.1.0"
     exon            3097..3299
                     /gene="Als2"
                     /gene_synonym="3222402C23Rik; 9430073A21Rik; Als2cr6;
                     Alsin; mKIAA1563"
                     /inference="alignment:Splign:2.1.0"
     exon            3300..3365
                     /gene="Als2"
                     /gene_synonym="3222402C23Rik; 9430073A21Rik; Als2cr6;
                     Alsin; mKIAA1563"
                     /inference="alignment:Splign:2.1.0"
     exon            3366..3464
                     /gene="Als2"
                     /gene_synonym="3222402C23Rik; 9430073A21Rik; Als2cr6;
                     Alsin; mKIAA1563"
                     /inference="alignment:Splign:2.1.0"
     exon            3465..3629
                     /gene="Als2"
                     /gene_synonym="3222402C23Rik; 9430073A21Rik; Als2cr6;
                     Alsin; mKIAA1563"
                     /inference="alignment:Splign:2.1.0"
     exon            3630..3741
                     /gene="Als2"
                     /gene_synonym="3222402C23Rik; 9430073A21Rik; Als2cr6;
                     Alsin; mKIAA1563"
                     /inference="alignment:Splign:2.1.0"
     exon            3742..3819
                     /gene="Als2"
                     /gene_synonym="3222402C23Rik; 9430073A21Rik; Als2cr6;
                     Alsin; mKIAA1563"
                     /inference="alignment:Splign:2.1.0"
     exon            3820..3953
                     /gene="Als2"
                     /gene_synonym="3222402C23Rik; 9430073A21Rik; Als2cr6;
                     Alsin; mKIAA1563"
                     /inference="alignment:Splign:2.1.0"
     exon            3954..4121
                     /gene="Als2"
                     /gene_synonym="3222402C23Rik; 9430073A21Rik; Als2cr6;
                     Alsin; mKIAA1563"
                     /inference="alignment:Splign:2.1.0"
     exon            4122..4239
                     /gene="Als2"
                     /gene_synonym="3222402C23Rik; 9430073A21Rik; Als2cr6;
                     Alsin; mKIAA1563"
                     /inference="alignment:Splign:2.1.0"
     exon            4240..4397
                     /gene="Als2"
                     /gene_synonym="3222402C23Rik; 9430073A21Rik; Als2cr6;
                     Alsin; mKIAA1563"
                     /inference="alignment:Splign:2.1.0"
     exon            4398..4520
                     /gene="Als2"
                     /gene_synonym="3222402C23Rik; 9430073A21Rik; Als2cr6;
                     Alsin; mKIAA1563"
                     /inference="alignment:Splign:2.1.0"
     exon            4521..4697
                     /gene="Als2"
                     /gene_synonym="3222402C23Rik; 9430073A21Rik; Als2cr6;
                     Alsin; mKIAA1563"
                     /inference="alignment:Splign:2.1.0"
     exon            4698..4743
                     /gene="Als2"
                     /gene_synonym="3222402C23Rik; 9430073A21Rik; Als2cr6;
                     Alsin; mKIAA1563"
                     /inference="alignment:Splign:2.1.0"
     exon            4744..4805
                     /gene="Als2"
                     /gene_synonym="3222402C23Rik; 9430073A21Rik; Als2cr6;
                     Alsin; mKIAA1563"
                     /inference="alignment:Splign:2.1.0"
     exon            4806..4955
                     /gene="Als2"
                     /gene_synonym="3222402C23Rik; 9430073A21Rik; Als2cr6;
                     Alsin; mKIAA1563"
                     /inference="alignment:Splign:2.1.0"
     exon            4956..5052
                     /gene="Als2"
                     /gene_synonym="3222402C23Rik; 9430073A21Rik; Als2cr6;
                     Alsin; mKIAA1563"
                     /inference="alignment:Splign:2.1.0"
     exon            5053..6512
                     /gene="Als2"
                     /gene_synonym="3222402C23Rik; 9430073A21Rik; Als2cr6;
                     Alsin; mKIAA1563"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6318..6323
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Als2"
                     /gene_synonym="3222402C23Rik; 9430073A21Rik; Als2cr6;
                     Alsin; mKIAA1563"
                     /note="hexamer: AATAAA"
     polyA_site      6338
                     /gene="Als2"
                     /gene_synonym="3222402C23Rik; 9430073A21Rik; Als2cr6;
                     Alsin; mKIAA1563"
                     /note="major polyA site"
ORIGIN      
        1 gaatcagctg atctcgcgta cccgcggtgc agtcgggctc gcgccgggag aagagcgcgg
       61 agctgcggga gcgtcaggtc ttgagagagc ttttgctaat gggatggttt ggtgatggag
      121 tactcctcct gaccgatgga ctcaaagaag aaaagctcaa cagaggcaga aggatccaaa
      181 gaaagaggcc tagtccatgt ctggcaggca ggatcctttt ctctaacacc agagaggttg
      241 ccaggctggg gtggaaagac agttcttcag gcagcccttg gtgtgaggca tggagttctt
      301 ctgactgaag atggtgaggt ctacagcttt gggactcttc cctggaaaag tgaatcagca
      361 gaaatttgtc caagcagccc ccttctagaa agtgccctgg ttgggcatca tgttattact
      421 gtggcaacag ggagcttcca cagtggagca gtgacagaga gcggggtggt gtacatgtgg
      481 ggagagaatg ctgccgggca gtgtgcggta gctaaccagc agtatgtgcc ggagccgagt
      541 cctgtcagca tttctgactc ggagaccagc ccgtcattag cagttaggat tctgcaattg
      601 gcatgtggcg aggaacacac actggcattg tcactcagca gagagatctg ggcatggggc
      661 accggctgtc agctgggcct catcaccacc actttcccag tgacaaagcc acagaaggtg
      721 gaacaccttg ctggacgcgt ggtgctccag gtggcctgcg gtgcattcca cagccttgca
      781 cttgtgcagt gcctccctcc tcaggatctg aagccagtcc cagagagatg caatcagtgc
      841 agccagctgc tcatcaccat gacagacaaa gaggaccatg tgataatatc ggacagccat
      901 tgctgccctt taggtgtgac attgtccgag tctcaagcag aaaagcatgc cagccctgct
      961 cccagccctc acccagaggc actggatgag cagggagagg tgtttgagaa cacggtggta
     1021 gaagctgaac tgaacatggg aagcagtcag accacaagtg gcagtgccat ttccacccag
     1081 cagaacgtcg tgggaacagc tgaagtgtct tctgccagaa cagctccgtc atacccagac
     1141 acccatgcgg taactgcata cctgcagaag ctgtcagagc attcgatgag ggagaaccat
     1201 gagcctggag aaaagccacc ccaggtccag cctcttgtag aagaagcagt tcctgatctt
     1261 cacagtccac caaccacaag cacctcagcc ctcaacagct tggtggtctc ctgtgcatct
     1321 gctgttggtg tcagagtggc tgccacctat gaagctgggg ccttgtctct caagaaagtt
     1381 atgaactttt acagcactgc cccctgcgag acggcagctc agtcgggcag tgcctccaca
     1441 ggcccagaaa gtctgaaaga tctccgagaa gagcaggtga aacaggagtc actgcaaggg
     1501 aagaaaagct caagtctcat ggacatcaga gaggaggagt tggagggagg gagtcgaaga
     1561 ctctccctcc cagggttgtt gtcgcaagtt tcccccaggc tcttaaggaa ggctgcgagg
     1621 gtgaaaactc ggacagtggt tctgactccc acatacagtg gagaagcaga tgcccttctg
     1681 ccttccctga ggacagaggt gtggacctgg gggaaaggca aggaagggca gctagggcac
     1741 ggcgacgtcc tgcccaggct tcagccgttg tgtgtcaagt gtctggatgg taaagaggta
     1801 atccacctgg aggcgggcgg ctcccactcc ctcgcactca ctgcgaaatc tcaggtttac
     1861 tcatggggca gtaatacctt tggtcagctt gggcattctg agtttccaac aacggttcct
     1921 cgactctcaa aggttagcag tgaaaatgga gtctggagtg tagctgcagg ccaagattat
     1981 tccttgtttt tagtggacac ggaagacttc cagcctgggt tgtattacag tggccgacag
     2041 gaccgtgcag aaggtgatac cctgccagag aatcccagtg gtacaaagac tccagtactt
     2101 ctctcctgta gtaagcttgg atacataagc agagtaacag caggaaaaga tagctatcta
     2161 gccttggtgg ataagaacat catgggatac atcgccagtc tccatgagtt ggcttctaca
     2221 gaaagacggt tttactcaaa actgagcgaa atcaaatcac agatacttag gcctcttctc
     2281 agtttagaaa atttgggcac agtgaccact gtccagctgt tgcaggaagt tgccagccgg
     2341 ttcagcaagt tgtgttacct cattgggcag catggagcct cactaagcag ctacctacag
     2401 ggcatgaagg aagccagcag cctggtcatc atgaagcact caagtctttt cctggacagc
     2461 tacacagagt actgcacatc agtttcaaat ttcctggtta tgggaggatt ccagcttctt
     2521 gctaagcctg ccattgattt cctaaataaa aaccaagaac tcttgcaaga tttgtcagaa
     2581 gtgaatgatg agaacactca gttgatggaa atcctgaaca tgctgttttt cttgccaatc
     2641 agacgacttc ataattatgc aaaagttttg ctaaagcttg ccacttgctt tgaagtgaca
     2701 tctccagagt atcaaaagct gcaggattcc agttcttgct atgagtctct tgctctccat
     2761 cttggcaaga agaggaagga agcagagtac acactgagct tctggaagac ctttcctggg
     2821 aaaatgacgg attccttgag gaagccagag cgccggctgc tgtgtgagag cagtaaccga
     2881 gccctctccc tgcagcatgc cggcaggttt tctgtgaatt ggttcattct cttcaatgat
     2941 gccctggtcc atgctcagtt ctctacacac cacgtgttcc ctttggccac actctgggca
     3001 gagccacttt ctgaagaagc tggtagcgtg aatggcttaa agataactac acctgaagaa
     3061 caattcacac tcatttcttc aacaccccag gaaaagacca agtggcttcg ggctatcagc
     3121 caagctgtgg atcaggcttt gagggggacg tccgatttcc cactttacgg aggcggcagc
     3181 agtgttcaga gacaggaacc acccatctca agaagtgcca aatacacttt ctacaaggat
     3241 actcgcctaa aggatgccac ttacgatggg cgctggcttt cagggaagcc tcatggcagg
     3301 ggtgttctga agtggcctga tggaaagatg tactctggca tgttcaggaa tggcttggaa
     3361 gatggatatg gtgaatacag aatccctaac aaggccctga acaaagaaga ccattatgta
     3421 ggccattgga aagaggggaa aatgtgtggg caaggagtct acagctatgc ctctggtgaa
     3481 gtgtttgaag gctgctttca agataacatg cgccatgggc atggtctgct ccggagtgga
     3541 aaactgactt cttcttctcc tagcatgttc attggccagt gggtaatgga taagaaagca
     3601 ggatatggcg tctttgatga tatcaccagg ggagaaaagt acatgggaat gtggcaggat
     3661 gatgtgtgcc aagggaatgg ggtagtagtc acccagtttg ggttatacta cgaaggcaac
     3721 ttccacctga ataagatgat gggaaatggg gttttgcttt ctgaagatga taccatctat
     3781 gaaggagaat tttccgatga ctggacactt agtggaaagg gaacgctgac tatgccacat
     3841 ggagattata ttgaaggtta ttttagtgga gaatggggat ctgggataaa aatcactggg
     3901 acctacttca aacctagcct gtatgagagc gataaggaca agcccaaagc cttcaggaag
     3961 ctggggaacc tggccgtggc agcagacgag aaatggagag cagtgtttga agaatgctgg
     4021 cgccagctgg gctgtgagag cccaggccaa ggggaggttt ggaaagcatg ggataatatt
     4081 gctgtggcct tgaccacgaa ccgtcgccag cataaagaca gtccagaaat actaagccgc
     4141 tctcagactc agaccctgga gagtttggag tacattcccc agcacattgg cgccttctct
     4201 gtggagaaat atgatgacat caagaagtat ttaataaagg cctgtgatac tcctctgcac
     4261 ccactgggca ggcttgtgga gaccctggtt gcggtgtata gaatgacata tgtgggtgta
     4321 ggggccaacc gccggttact gcaggaagct gtgaaggaga ttaaatctta tctcaagagg
     4381 attttccagc ttgtgaggtt cttgtttcct gagcttcctg aggagggcag cacaattcct
     4441 ctttctgctc ctctgcccac tggaaggaga tccttctgta ctggaaaatc ggattccaga
     4501 tccgagtcac cagaaccagg ttatgtagta acaagttctg gcttactgct tccggtgctg
     4561 ctgccacggc tctacccacc tctcttcatg ctctatgccc tggataatga ccgagaggaa
     4621 gacatttact gggaatgtgt gcttcgacta aacaagcagc cagatattgc tctcttgggc
     4681 ttccttggag tacagaaaaa attctggcca gccaccttgt caatccttgg agagagtaaa
     4741 aaggtgttgt caaccacaaa ggatgcttgc tttgcatctg cagtagaatg cctgcagcag
     4801 atcagcacaa catttactcc atcagacaag cttaaagtga tccagcagac ctttgaagag
     4861 atctcccaga gtgtccttgc atcgctgcag gaggacttcc tctggtccat ggatgacttg
     4921 ttccccgtct tcttatacgt ggtgctgcgg gccaggattc ggaacttggg ctctgaagtt
     4981 cacctcattg aggatctgat ggaccccttt ctccagcatg gggaacaagg catcatgttc
     5041 accaccttga aggcctgtta cttccagatt cagcgggaga agcttaacta gggcgcctga
     5101 cagcttgagg accggattat ctgctgcgga ggctacagct atggcacagg caccgactgg
     5161 aggctgatgg ggcaaagaac agtgttgaat acagaatgga cttttgtgct attttggttg
     5221 taatttctga gccttactaa taattagagc ccagcatgga aaacatactg tatcattcaa
     5281 atggagactg gaaaaggaga tagggataga gtagagtctt tggcctgtgc tgagatccac
     5341 acacctactt agaaaaggaa actggttacc ctttcctgta gtgaaagctc tcagctccat
     5401 gcagttccag gaaacctttc caggaaagct gcttagatga aaagaagttg atgactgtgt
     5461 ttaagctcct ggtttgtcta attccatttg cagttaccca ataccctttg gcaaggagca
     5521 ggttttactt gaactgaagc agccatccct tgccttccta gacctctccc aggcacaagt
     5581 gcagcatgct actttgctag gggtgggggt gggggagaag aagttttaaa ctgtagtttt
     5641 aaccttttgt aagccccttt accaaggcat ttgtggtcag agagctccca cggggtgact
     5701 atgacatcct ggtcccctcg tggaatgcat ccacatagga tcttctgcct gctgactgaa
     5761 aagaacatag gaatacactg gagtgcaaac actgccgtgc caagctgctc caaacctcac
     5821 tgatccgagg cccactgcct acccaggagg cccgtaagct tcttagcaca agctttgtgt
     5881 ggagactgaa gatctgcaca tgtgaggaag cagggagcta cagtggccct cagcccagtc
     5941 tgcgggtctt ccctctacct cacactgaac tcagaaggga aggaaggaga gacgcacatg
     6001 ggattctccc acctcagaag tattgtgaca gcaccgcata accacggttt gctcttttac
     6061 aagcagcctc acaagtgtgg gttgtgggtg tgcgctggag cagtgccact cgtagctgtt
     6121 tggataccac aggtgctctt ctgtctcatc tgctgtactc ggaggcgagc gcagtggcct
     6181 gactcatggg aaatgactca gcaggcggca actacttttg aaaaggatca tgatttccga
     6241 gctactttat aatcaagaca agcatttgtt aacatactgt tttggaatgt tggctgtaat
     6301 gtaacagcag ttttcataat aaatgacatt catctctacg aagctgttct atgactgtgg
     6361 aagaaatgac atggggcaga ctgtacttta aagagtgctc agtttacagt attctttctc
     6421 cacattgtaa tccatgcctc agacctcagg aacccctggg gtttttaatt cacttaacta
     6481 ctagccaatt tctggcaaag agcaagttaa tt
//
