LOCUS       NM_009661               3273 bp    mRNA    linear   ROD 03-JUN-2024
DEFINITION  Mus musculus arachidonate 8-lipoxygenase (Alox8), mRNA.
ACCESSION   NM_009661 XM_126384
VERSION     NM_009661.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3273)
  AUTHORS   Schafer,M., Reisch,F., Labuz,D., Machelska,H., Stehling,S.,
            Puschel,G.P., Rothe,M., Heydeck,D. and Kuhn,H.
  TITLE     Humanization of the Reaction Specificity of Mouse Alox15b Inversely
            Modified the Susceptibility of Corresponding Knock-In Mice in Two
            Different Animal Inflammation Models
  JOURNAL   Int J Mol Sci 24 (13), 11034 (2023)
   PUBMED   37446212
  REMARK    GeneRIF: Humanization of the Reaction Specificity of Mouse Alox15b
            Inversely Modified the Susceptibility of Corresponding Knock-In
            Mice in Two Different Animal Inflammation Models.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3273)
  AUTHORS   Yli-Karjanmaa,M., Larsen,K.S., Fenger,C.D., Kristensen,L.K.,
            Martin,N.A., Jensen,P.T., Breton,A., Nathanson,L., Nielsen,P.V.,
            Lund,M.C., Carlsen,S.L., Gramsbergen,J.B., Finsen,B., Stubbe,J.,
            Frich,L.H., Stolp,H., Brambilla,R., Anthony,D.C., Meyer,M. and
            Lambertsen,K.L.
  TITLE     TNF deficiency causes alterations in the spatial organization of
            neurogenic zones and alters the number of microglia and neurons in
            the cerebral cortex
  JOURNAL   Brain Behav Immun 82, 279-297 (2019)
   PUBMED   31505254
REFERENCE   3  (bases 1 to 3273)
  AUTHORS   Xiao,Y., Song,C., Lin,Q., Shi,X., Yu,W., Huang,X., Wang,H.,
            Chen,Y., Wang,R., Geng,X., Qin,M., Hu,K., Fan,Y., Qiao,Y., Gao,E.,
            Zhao,W. and Chang,J.
  TITLE     Cardioprotection of (+/-)-sodium 5-bromo-2-(alpha-hydroxypentyl)
            benzoate (BZP) on mouse myocardium I/R injury through inhibiting
            12/15-LOX-2 activity
  JOURNAL   J Mol Cell Cardiol 135, 52-66 (2019)
   PUBMED   31362020
  REMARK    GeneRIF: Our findings, for the first time, strongly indicate that
            1a may serve as a potent and promising cardioprotective agent in
            treatment of ischemia-reperfusion (I/R) injury, at least partially
            through targeting 12/15-LOX-2.
REFERENCE   4  (bases 1 to 3273)
  AUTHORS   van Eif,V.W.W., Stefanovic,S., van Duijvenboden,K., Bakker,M.,
            Wakker,V., de Gier-de Vries,C., Zaffran,S., Verkerk,A.O.,
            Boukens,B.J. and Christoffels,V.M.
  TITLE     Transcriptome analysis of mouse and human sinoatrial node cells
            reveals a conserved genetic program
  JOURNAL   Development 146 (8) (2019)
   PUBMED   30936179
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3273)
  AUTHORS   Alfardan,R., Guo,C., Toth,L.A. and Nie,D.
  TITLE     Impaired Recovery from Influenza A/X-31(H3N2) Infection in Mice
            with 8-Lipoxygenase Deficiency
  JOURNAL   Med Sci (Basel) 7 (4), 60 (2019)
   PUBMED   31013822
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3273)
  AUTHORS   Kim,E., Muga,S.J. and Fischer,S.M.
  TITLE     Identification and characterization of a phorbol ester-responsive
            element in the murine 8S-lipoxygenase gene
  JOURNAL   J Biol Chem 279 (12), 11188-11197 (2004)
   PUBMED   14711820
REFERENCE   7  (bases 1 to 3273)
  AUTHORS   Krieg,P., Kinzig,A., Heidt,M., Marks,F. and Furstenberger,G.
  TITLE     cDNA cloning of a 8-lipoxygenase and a novel epidermis-type
            lipoxygenase from phorbol ester-treated mouse skin
  JOURNAL   Biochim Biophys Acta 1391 (1), 7-12 (1998)
   PUBMED   9518531
REFERENCE   8  (bases 1 to 3273)
  AUTHORS   Jisaka,M., Kim,R.B., Boeglin,W.E., Nanney,L.B. and Brash,A.R.
  TITLE     Molecular cloning and functional expression of a phorbol
            ester-inducible 8S-lipoxygenase from mouse skin
  JOURNAL   J Biol Chem 272 (39), 24410-24416 (1997)
   PUBMED   9305900
REFERENCE   9  (bases 1 to 3273)
  AUTHORS   Furstenberger,G., Hagedorn,H., Jacobi,T., Besemfelder,E.,
            Stephan,M., Lehmann,W.D. and Marks,F.
  TITLE     Characterization of an 8-lipoxygenase activity induced by the
            phorbol ester tumor promoter 12-O-tetradecanoylphorbol-13-acetate
            in mouse skin in vivo
  JOURNAL   J Biol Chem 266 (24), 15738-15745 (1991)
   PUBMED   1874732
REFERENCE   10 (bases 1 to 3273)
  AUTHORS   Fischer,S.M., Baldwin,J.K., Jasheway,D.W., Patrick,K.E. and
            Cameron,G.S.
  TITLE     Phorbol ester induction of 8-lipoxygenase in inbred SENCAR (SSIN)
            but not C57BL/6J mice correlated with hyperplasia, edema, and
            oxidant generation but not ornithine decarboxylase induction
  JOURNAL   Cancer Res 48 (3), 658-664 (1988)
   PUBMED   3335028
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL645527.20 and AK028724.1.
            
            On Jan 26, 2013 this sequence version replaced NM_009661.3.
            
            Summary: This gene belongs to the lipoxygenase (LOX) gene family
            whose members encode enzymes that catalyze the addition of
            molecular oxygen to polyunsaturated fatty acids (PUFAs) to yield
            fatty acid hydroperoxides. The encoded enzyme preferentially
            metabolizes arachidonic acid to yield 8-hydroxyeicosatetraenoic
            acid (8-HETE), while metabolizing linoleic acid less efficiently.
            The gene may also function as a tumor suppressor. This gene is
            located in a cluster of related genes that spans approximately 75
            kilobases on chromosome 11. [provided by RefSeq, Jan 2013].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK028724.1, BC015253.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164136 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-6                 AL645527.20        114401-114406       c
            7-3273              AK028724.1         3-3269
FEATURES             Location/Qualifiers
     source          1..3273
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 42.38 cM"
     gene            1..3273
                     /gene="Alox8"
                     /gene_synonym="15-LOX-2; 15-LOX-B; 8-LOX; 8S-LOX; Alox15b"
                     /note="arachidonate 8-lipoxygenase"
                     /db_xref="GeneID:11688"
                     /db_xref="MGI:MGI:1098228"
     exon            1..181
                     /gene="Alox8"
                     /gene_synonym="15-LOX-2; 15-LOX-B; 8-LOX; 8S-LOX; Alox15b"
                     /inference="alignment:Splign:2.1.0"
     exon            182..404
                     /gene="Alox8"
                     /gene_synonym="15-LOX-2; 15-LOX-B; 8-LOX; 8S-LOX; Alox15b"
                     /inference="alignment:Splign:2.1.0"
     exon            405..486
                     /gene="Alox8"
                     /gene_synonym="15-LOX-2; 15-LOX-B; 8-LOX; 8S-LOX; Alox15b"
                     /inference="alignment:Splign:2.1.0"
     exon            487..609
                     /gene="Alox8"
                     /gene_synonym="15-LOX-2; 15-LOX-B; 8-LOX; 8S-LOX; Alox15b"
                     /inference="alignment:Splign:2.1.0"
     exon            610..713
                     /gene="Alox8"
                     /gene_synonym="15-LOX-2; 15-LOX-B; 8-LOX; 8S-LOX; Alox15b"
                     /inference="alignment:Splign:2.1.0"
     exon            714..886
                     /gene="Alox8"
                     /gene_synonym="15-LOX-2; 15-LOX-B; 8-LOX; 8S-LOX; Alox15b"
                     /inference="alignment:Splign:2.1.0"
     exon            887..1033
                     /gene="Alox8"
                     /gene_synonym="15-LOX-2; 15-LOX-B; 8-LOX; 8S-LOX; Alox15b"
                     /inference="alignment:Splign:2.1.0"
     exon            1034..1237
                     /gene="Alox8"
                     /gene_synonym="15-LOX-2; 15-LOX-B; 8-LOX; 8S-LOX; Alox15b"
                     /inference="alignment:Splign:2.1.0"
     exon            1238..1324
                     /gene="Alox8"
                     /gene_synonym="15-LOX-2; 15-LOX-B; 8-LOX; 8S-LOX; Alox15b"
                     /inference="alignment:Splign:2.1.0"
     exon            1325..1494
                     /gene="Alox8"
                     /gene_synonym="15-LOX-2; 15-LOX-B; 8-LOX; 8S-LOX; Alox15b"
                     /inference="alignment:Splign:2.1.0"
     exon            1495..1616
                     /gene="Alox8"
                     /gene_synonym="15-LOX-2; 15-LOX-B; 8-LOX; 8S-LOX; Alox15b"
                     /inference="alignment:Splign:2.1.0"
     exon            1617..1717
                     /gene="Alox8"
                     /gene_synonym="15-LOX-2; 15-LOX-B; 8-LOX; 8S-LOX; Alox15b"
                     /inference="alignment:Splign:2.1.0"
     exon            1718..1888
                     /gene="Alox8"
                     /gene_synonym="15-LOX-2; 15-LOX-B; 8-LOX; 8S-LOX; Alox15b"
                     /inference="alignment:Splign:2.1.0"
     exon            1889..3273
                     /gene="Alox8"
                     /gene_synonym="15-LOX-2; 15-LOX-B; 8-LOX; 8S-LOX; Alox15b"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2889..2894
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Alox8"
                     /gene_synonym="15-LOX-2; 15-LOX-B; 8-LOX; 8S-LOX; Alox15b"
                     /note="hexamer: TATAAA"
     polyA_site      2907
                     /gene="Alox8"
                     /gene_synonym="15-LOX-2; 15-LOX-B; 8-LOX; 8S-LOX; Alox15b"
                     /note="major polyA site"
ORIGIN      
        1 cagcttgcag tagagagcta aactggtcag gaggatggcg aaatgcaggg tgagagtatc
       61 cacgggggaa gcctgtgggg ctggcacatg ggacaaagtg tctgtcagca tcgtgggaac
      121 ccacggagag agccccttag tacctctgga ccatctgggc aaggagttca gcgccggtgc
      181 tgaagaagac ttcgaggtga cgcttcccca ggacgtaggc actgtgctga tgctgcgagt
      241 ccacaaagca cccccggaag tgtccctccc gcttatgtct ttccgttctg atgcctggtt
      301 ctgccgctgg ttcgagctgg agtggctacc tggggctgca ctccacttcc cctgttatca
      361 gtggctggaa ggggcggggg agctggtgct gagagaggga gcagcaaagg tgtcctggca
      421 agaccatcac cctacactgc aggatcagcg ccagaaggag cttgagtcca ggcagaagat
      481 gtacagctgg aagacttaca ttgaaggttg gcctcgctgc cttgaccacg agactgtgaa
      541 agacttggac ctcaacatca agtactctgc gatgaagaat gccaaactct tctttaaagc
      601 ccactccgcg tatacggagc tgaaagtcaa agggctcctg gaccgcacag gactctggag
      661 gagtctgagg gagatgagaa ggctgtttaa cttccgcaag actccagcag cagagtatgt
      721 gtttgcacac tggcaggaag atgccttctt cgcctcccag ttcctaaatg gcatcaaccc
      781 ggtcctgatt cgccgctgtc acagtctccc aaacaacttc ccggtcactg atgaaatggt
      841 ggccccagtg ctgggccctg gaaccagtct gcaggctgag ttggagaagg gctccctgtt
      901 cttggtggat catggcattc tttctggagt ccacaccaac atcctcaatg gaaagcctca
      961 gttctctgca gccccgatga ccctgttaca ccagagctca gggtccggac ccctgcttcc
     1021 cattgccatc cagctcaaac agactcccgg gccagacaac cccatcttcc tgcccagcga
     1081 tgacacgtgg gactggttgc tggccaagac ctgggttcgc aattctgagt tttacatcca
     1141 tgaggctgtc acacatctgc tgcatgccca tctgattcca gaagtctttg ccttggccac
     1201 attacgtcag ctgcctaggt gtcaccctct cttcaagcta ttgattcctc acattcggta
     1261 cacactgcac atcaacacgc ttgcccggga gctgctcgtt gcccctggga agttgataga
     1321 caagtccaca ggccttggca ctgggggatt ctctgacctg ataaagagaa acatggagca
     1381 gctgaactac tctgtcctgt gtctccctga agatatccga gcccgaggtg tggaagacat
     1441 cccaggctac tattaccgag atgatgggat gcagatctgg ggggcaataa agagctttgt
     1501 ctctgaaata gtcagcatct actatccaag tgacacatcc gtccaagatg accaagagct
     1561 ccaggcctgg gtgagggaga tcttctctga gggcttcctc ggccgagaaa gctcaggtat
     1621 gccctccttg ttggataccc gggaagccct ggtccagtat atcaccatgg tgatattcac
     1681 ctgctcagcc aagcatgcag ctgtcagttc aggccagttc gactcttgtg tttggatgcc
     1741 caatctgcca cctaccatgc agctaccacc acctacttcc aaaggccagg cccggcctga
     1801 gagtttcata gccacgctcc cagcagttaa ttcgtcaagt tatcacatca ttgctctctg
     1861 gctgctaagc gcagaacctg gggaccaaag gcccctgggc cactatccag atgaacactt
     1921 cacagaggat gccccccggc gaagcgtggc tgccttccag agaaagctga tccagatctc
     1981 caagggcatc agggagagga accgaggcct ggcactgccc tacacctacc tggatcctcc
     2041 cctcattgag aacagtgtct ccatctaaca tcttggagaa gacagtcctg tgtgacatat
     2101 agaactcttg accatgcctc tccaggctaa gtccccgtat gcttctcctg gacaaccaag
     2161 ccccatctta cacacacaca cacacacaca cacctaataa aatcgaaaca gaaaaaccta
     2221 aactcccaca gaaggcaaga tctcacacag cagagagcca tccaaatgtt tggagaccct
     2281 gagcttcagc tctgattaac ggctttgctg gtttgctttg ctttctattc cattaaccat
     2341 ggacggtaac agaaagcaca gaaccctggt tcactgcaca aagccactga gatctcaccc
     2401 tcacctgaca caaaggcagc tatcatacag gcttatcagg aacacaggaa tttgtccaat
     2461 caaagcctac ccactaggtc catcgtgacc tacgacctca cactggcatg ctttagcttt
     2521 gagaagggat tactggagtc aggtacgaag agaaggacag gacgaaggca tggctccatg
     2581 tggaagaaca tatctgctct tccagatgac cagggtagct cacagccatg tgtcattcta
     2641 actccagagg tctctagtgg ccatgaagac tccaggcatt caggggatat accagtagac
     2701 accaaaatta tactttttaa gagagaggaa tgggctggag agatggctca gcggttaaga
     2761 gcactgactg ctcttccaga gatcctgagt tcaattccca gcaaccacat ggtggctcac
     2821 aaccatctgt aatgggattc gatgccctct tctggcgtgt ctgaagacag cgacagtgta
     2881 tgcacatata taaaataaat aaatctttaa aaaacaaaac aagagagagg gacatgctac
     2941 catttctacc tcacttcttc tcaaagccac ccctaaagtg aattgtgaac caggtcccct
     3001 ttgcagagag ttagaagata ttctcaaacc tctaatacct tcacatctaa aatccatctt
     3061 cattccaaaa ttccaatatt ttatatacac tctccagttt ggtgggtgag gggttgtttt
     3121 ttgtttggtt tggtttggtt ggggttttgt ttttgttttt gattttgttt ttctctggtt
     3181 cagactccat ggacgttcat taatgtcata aatgagttca ttccaagttg cctggtgatt
     3241 ttcatattac aggcatcaaa gttttggatc ttc
//
