LOCUS       NM_009660               2414 bp    mRNA    linear   ROD 14-JAN-2025
DEFINITION  Mus musculus arachidonate 15-lipoxygenase (Alox15), mRNA.
ACCESSION   NM_009660
VERSION     NM_009660.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Kain,V., Grilo,G.A., Upadhyay,G., Nadler,J.L., Serhan,C.N. and
            Halade,G.V.
  TITLE     Macrophage-specific lipoxygenase deletion amplify cardiac repair
            activating Treg cells in chronic heart failure
  JOURNAL   J Leukoc Biol 116 (4), 864-875 (2024)
   PUBMED   38785336
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Taketomi,Y., Higashi,T., Kano,K., Miki,Y., Mochizuki,C.,
            Toyoshima,S., Okayama,Y., Nishito,Y., Nakae,S., Tanaka,S.,
            Tokuoka,S.M., Oda,Y., Shichino,S., Ueha,S., Matsushima,K.,
            Akahoshi,N., Ishii,S., Chun,J., Aoki,J. and Murakami,M.
  TITLE     Lipid-orchestrated paracrine circuit coordinates mast cell
            maturation and anaphylaxis through functional interaction with
            fibroblasts
  JOURNAL   Immunity 57 (8), 1828-1847 (2024)
   PUBMED   39002541
REFERENCE   3  (bases 1 to 2414)
  AUTHORS   Chen,Y., Liu,Z., Chen,H., Wen,Y., Fan,L. and Luo,M.
  TITLE     Rhythm gene PER1 mediates ferroptosis and lipid metabolism through
            SREBF2/ALOX15 axis in polycystic ovary syndrome
  JOURNAL   Biochim Biophys Acta Mol Basis Dis 1870 (5), 167182 (2024)
   PUBMED   38653359
  REMARK    GeneRIF: Rhythm gene PER1 mediates ferroptosis and lipid metabolism
            through SREBF2/ALOX15 axis in polycystic ovary syndrome.
REFERENCE   4  (bases 1 to 2414)
  AUTHORS   Zhang,W., Huang,F., Ding,X., Qin,J., Wang,W. and Luo,L.
  TITLE     Identifying ALOX15-initiated lipid peroxidation increases
            susceptibility to ferroptosis in asthma epithelial cells
  JOURNAL   Biochim Biophys Acta Mol Basis Dis 1870 (5), 167176 (2024)
   PUBMED   38641013
  REMARK    GeneRIF: Identifying ALOX15-initiated lipid peroxidation increases
            susceptibility to ferroptosis in asthma epithelial cells.
REFERENCE   5  (bases 1 to 2414)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   6  (bases 1 to 2414)
  AUTHORS   Sun,D. and Funk,C.D.
  TITLE     Disruption of 12/15-lipoxygenase expression in peritoneal
            macrophages. Enhanced utilization of the 5-lipoxygenase pathway and
            diminished oxidation of low density lipoprotein
  JOURNAL   J Biol Chem 271 (39), 24055-24062 (1996)
   PUBMED   8798642
REFERENCE   7  (bases 1 to 2414)
  AUTHORS   van Dijk,K.W., Steketee,K., Havekes,L., Frants,R. and Hofker,M.
  TITLE     Genomic and cDNA cloning of a novel mouse lipoxygenase gene
  JOURNAL   Biochim Biophys Acta 1259 (1), 4-8 (1995)
   PUBMED   7492614
REFERENCE   8  (bases 1 to 2414)
  AUTHORS   Krieg,P., Kinzig,A., Ress-Loschke,M., Vogel,S., Vanlandingham,B.,
            Stephan,M., Lehmann,W.D., Marks,F. and Furstenberger,G.
  TITLE     12-Lipoxygenase isoenzymes in mouse skin tumor development
  JOURNAL   Mol Carcinog 14 (2), 118-129 (1995)
   PUBMED   7576099
REFERENCE   9  (bases 1 to 2414)
  AUTHORS   Freire-Moar,J., Alavi-Nassab,A., Ng,M., Mulkins,M. and Sigal,E.
  TITLE     Cloning and characterization of a murine macrophage lipoxygenase
  JOURNAL   Biochim Biophys Acta 1254 (1), 112-116 (1995)
   PUBMED   7811740
REFERENCE   10 (bases 1 to 2414)
  AUTHORS   Chen,X.S., Kurre,U., Jenkins,N.A., Copeland,N.G. and Funk,C.D.
  TITLE     cDNA cloning, expression, mutagenesis of C-terminal isoleucine,
            genomic structure, and chromosomal localizations of murine
            12-lipoxygenases
  JOURNAL   J Biol Chem 269 (19), 13979-13987 (1994)
   PUBMED   8188678
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK142371.1, U04331.1 and AL596096.7.
            
            On Mar 31, 2007 this sequence version replaced NM_009660.2.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data because no single transcript from the same
            strain was available for the full length of the gene. The extent of
            this transcript is supported by transcript alignments and
            orthologous data.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK142371.1, L34570.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-26                AK142371.1         2-27
            27-2018             U04331.1           1-1992
            2019-2414           AL596096.7         26725-27120         c
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 42.99 cM"
     gene            1..2414
                     /gene="Alox15"
                     /gene_synonym="12-LO; 12/15-LO; 15-LOX; Alox12l; L-12LO"
                     /note="arachidonate 15-lipoxygenase"
                     /db_xref="GeneID:11687"
                     /db_xref="MGI:MGI:87997"
     exon            1..161
                     /gene="Alox15"
                     /gene_synonym="12-LO; 12/15-LO; 15-LOX; Alox12l; L-12LO"
                     /inference="alignment:Splign:2.1.0"
     CDS             27..2018
                     /gene="Alox15"
                     /gene_synonym="12-LO; 12/15-LO; 15-LOX; Alox12l; L-12LO"
                     /EC_number="1.13.11.31"
                     /EC_number="1.13.11.33"
                     /EC_number="1.13.11.12"
                     /note="arachidonate 12-lipoxygenase, leukocyte-type;
                     linoleate 13S-lipoxygenase; hepoxilin A3 synthase Alox15;
                     12-LOX; 12/15-lipoxygenase; arachidonate omega-6
                     lipoxygenase"
                     /codon_start=1
                     /product="polyunsaturated fatty acid lipoxygenase ALOX15"
                     /protein_id="NP_033790.3"
                     /db_xref="CCDS:CCDS24944.1"
                     /db_xref="GeneID:11687"
                     /db_xref="MGI:MGI:87997"
                     /translation="MGVYRIRVSTGDSVYAGSNNEVYLWLIGQHGEASLGKLFRPCRN
                     SEAEFKVDVSEYLGPLLFVRVQKWHYLKEDAWFCNWISVKGPGDQGSEYTFPCYRWVQ
                     GTSILNLPEGTGCTVVEDSQGLFRNHREEELEERRSLYRWGNWKDGTILNVAATSISD
                     LPVDQRFREDKRLEFEASQVLGTMDTVINFPKNTVTCWKSLDDFNYVFKSGHTKMAER
                     VRNSWKEDAFFGYQFLNGANPMVLKRSTCLPARLVFPPGMEKLQAQLDEELKKGTLFE
                     ADFFLLDGIKANVILCSQQYLAAPLVMLKLQPDGQLLPIAIQLELPKTGSTPPPIFTP
                     LDPPMDWLLAKCWVRSSDLQLHELQAHLLRGHLVAEVFAVATMRCLPSVHPVFKLLVP
                     HLLYTMEINVRARSDLISERGFFDKVMSTGGGGHLDLLKQAGAFLTYSSLCPPDDLAE
                     RGLLDIDTCFYAKDALQLWQVMNRYVVGMFDLYYKTDQAVQDDYELQSWCQEITEIGL
                     QGAQDRGFPTSLQSRAQACHFITMCIFTCTAQHSSIHLGQLDWFYWVPNAPCTMRLPP
                     PKTKDATMEKLMATLPNPNQSTLQINVVWLLGRRQAVMVPLGQHSEEHFPNPEAKAVL
                     KKFREELAALDKEIEIRNKSLDIPYEYLRPSLVENSVAI"
     exon            162..366
                     /gene="Alox15"
                     /gene_synonym="12-LO; 12/15-LO; 15-LOX; Alox12l; L-12LO"
                     /inference="alignment:Splign:2.1.0"
     exon            367..448
                     /gene="Alox15"
                     /gene_synonym="12-LO; 12/15-LO; 15-LOX; Alox12l; L-12LO"
                     /inference="alignment:Splign:2.1.0"
     exon            449..571
                     /gene="Alox15"
                     /gene_synonym="12-LO; 12/15-LO; 15-LOX; Alox12l; L-12LO"
                     /inference="alignment:Splign:2.1.0"
     exon            572..675
                     /gene="Alox15"
                     /gene_synonym="12-LO; 12/15-LO; 15-LOX; Alox12l; L-12LO"
                     /inference="alignment:Splign:2.1.0"
     exon            676..836
                     /gene="Alox15"
                     /gene_synonym="12-LO; 12/15-LO; 15-LOX; Alox12l; L-12LO"
                     /inference="alignment:Splign:2.1.0"
     exon            837..980
                     /gene="Alox15"
                     /gene_synonym="12-LO; 12/15-LO; 15-LOX; Alox12l; L-12LO"
                     /inference="alignment:Splign:2.1.0"
     exon            981..1190
                     /gene="Alox15"
                     /gene_synonym="12-LO; 12/15-LO; 15-LOX; Alox12l; L-12LO"
                     /inference="alignment:Splign:2.1.0"
     exon            1191..1277
                     /gene="Alox15"
                     /gene_synonym="12-LO; 12/15-LO; 15-LOX; Alox12l; L-12LO"
                     /inference="alignment:Splign:2.1.0"
     exon            1278..1447
                     /gene="Alox15"
                     /gene_synonym="12-LO; 12/15-LO; 15-LOX; Alox12l; L-12LO"
                     /inference="alignment:Splign:2.1.0"
     exon            1448..1569
                     /gene="Alox15"
                     /gene_synonym="12-LO; 12/15-LO; 15-LOX; Alox12l; L-12LO"
                     /inference="alignment:Splign:2.1.0"
     exon            1570..1670
                     /gene="Alox15"
                     /gene_synonym="12-LO; 12/15-LO; 15-LOX; Alox12l; L-12LO"
                     /inference="alignment:Splign:2.1.0"
     exon            1671..1838
                     /gene="Alox15"
                     /gene_synonym="12-LO; 12/15-LO; 15-LOX; Alox12l; L-12LO"
                     /inference="alignment:Splign:2.1.0"
     exon            1839..2414
                     /gene="Alox15"
                     /gene_synonym="12-LO; 12/15-LO; 15-LOX; Alox12l; L-12LO"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2392..2397
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Alox15"
                     /gene_synonym="12-LO; 12/15-LO; 15-LOX; Alox12l; L-12LO"
                     /note="hexamer: AATAAA"
     polyA_site      2412
                     /gene="Alox15"
                     /gene_synonym="12-LO; 12/15-LO; 15-LOX; Alox12l; L-12LO"
                     /note="major polyA site"
ORIGIN      
        1 agaagggaga aaacttcatc tgcaagatgg gtgtctaccg catccgcgtc tccaccgggg
       61 actccgtgta cgcgggctcc aacaacgagg tctacctgtg gttgatcgga cagcatgggg
      121 aggcatctct cgggaagctg ttccgaccct gtcggaactc ggaggcagaa ttcaaggtgg
      181 atgtgtcaga ataccttggg ccactgctgt ttgtgagagt gcagaaatgg cattatctca
      241 aagaggacgc ctggttctgc aactggattt ctgtgaaggg ccccggagac cagggatcgg
      301 agtacacgtt cccctgttac cgatgggttc agggcaccag catcctgaac ctccctgagg
      361 gcactggctg caccgtggtt gaagactctc aaggcctgtt caggaaccac agggaggagg
      421 aactggaaga aaggaggagt ctgtacaggt ggggcaactg gaaggatggc acaatcctga
      481 acgtggcggc gaccagtatc tctgacctcc ctgtagacca gcgatttcga gaggacaaaa
      541 gacttgaatt tgaagcttca caggttctgg ggacaatgga caccgttatt aacttcccta
      601 aaaacactgt gacctgctgg aaaagcctag atgacttcaa ctatgttttc aagagtggcc
      661 acaccaagat ggctgagcgg gttcgaaact cctggaaaga ggatgctttc tttgggtacc
      721 agtttctcaa tggtgctaac cccatggtgc tgaagcggtc tacttgtctc cctgcccgcc
      781 tggtattccc tccggggatg gagaagctac aggcccagct ggatgaggag ctcaagaaag
      841 gcactctgtt tgaagcggat ttcttccttc tggatgggat caaggccaat gtcatccttt
      901 gtagtcagca gtacctggct gcccccctcg tcatgctgaa gctgcagccc gatgggcaac
      961 tcttgcctat agccatccag ctcgaactgc ccaaaactgg gtctacccca ccgccgattt
     1021 tcacgccctt ggatccccca atggactggc tcctggccaa atgctgggtc cgaagctcag
     1081 acttacagct tcatgagcta caggctcatc ttctgagggg acacttggtg gctgaggtct
     1141 ttgctgtggc caccatgagg tgcctgcctt ccgtgcaccc tgtttttaag cttctagttc
     1201 ctcacctact ctacaccatg gaaatcaatg tccgggccag gagcgacctg atctcagaga
     1261 gaggcttttt tgacaaggtg atgagcacag gtggaggagg ccacctggat cttctcaagc
     1321 aagctggagc cttcctgacc tacagctcat tgtgtccccc tgatgacttg gctgagcgag
     1381 gactcctgga tattgacact tgcttctatg ctaaagatgc cctgcagctc tggcaagtca
     1441 tgaatcggta cgtggtggga atgttcgatc tctactacaa gaccgaccaa gctgttcagg
     1501 atgactatga actgcagagc tggtgtcaag agatcactga gattgggttg caaggtgccc
     1561 aggacagagg cttccccacc tccctccagt cccgggctca ggcttgccac ttcatcacca
     1621 tgtgcatctt cacatgcact gcgcagcact cttccatcca tcttggccag ctggattggt
     1681 tctactgggt tcctaatgca ccctgcacca tgcggctgcc accacctaaa accaaggacg
     1741 cgacgatgga gaagctgatg gcgacgctgc ccaatcctaa tcagtctact ctccagataa
     1801 atgtcgtttg gctcctgggc agacgccagg ctgttatggt gcccctgggc cagcattcag
     1861 aggaacactt cccaaaccct gaggccaaag ctgtgctgaa gaagttcaga gaggagctgg
     1921 ctgccttgga taaggaaatt gagattcgta acaagagctt ggacatacct tatgagtacc
     1981 tgcggcccag cctggtagaa aacagcgtgg ccatatgacc atcccccctc tcgatttgtt
     2041 atttaatcaa gaccactcaa gtgcatccct gtgcttgagc cccgccctct gccagtgtgg
     2101 aacccttttc caagagcccg ctttccatag tctgctgtag tgaacacatt ttgctttgga
     2161 tgcgttaccc agagtgccac ttttcattca tcctttctgc ttttccttcc ccatagggta
     2221 aataatagcc atgcttggga aaagcacccc gggaacacaa aagggtgata atatacattg
     2281 ttacacatcg tctatgaatc aaatagatct caagccttaa tagagtctaa tagcaacaat
     2341 aaaagatgtt ttgggctagt gatagaaaag aacagggacc taattatttt aaataaaatg
     2401 ttgcaaagat tata
//
