LOCUS       NM_009659               2347 bp    mRNA    linear   ROD 04-JUN-2024
DEFINITION  Mus musculus arachidonate 12-lipoxygenase, 12R type (Alox12b),
            mRNA.
ACCESSION   NM_009659 XM_908612
VERSION     NM_009659.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2347)
  AUTHORS   Meyer,J.M., Vavrova,K., Radner,F.P.W., Schneider,H., Dick,A.,
            Mauro,T.M. and Elias,P.M.
  TITLE     ALOX12B and PNPLA1 Have Distinct Roles in Epidermal Lipid Lamellar
            Organization
  JOURNAL   J Invest Dermatol 143 (2), 332-335 (2023)
   PUBMED   36116510
  REMARK    GeneRIF: ALOX12B and PNPLA1 Have Distinct Roles in Epidermal Lipid
            Lamellar Organization.
REFERENCE   2  (bases 1 to 2347)
  AUTHORS   Meyer,J.M., Crumrine,D., Schneider,H., Dick,A., Schmuth,M.,
            Gruber,R., Radner,F.P.W., Grond,S., Wakefield,J.S., Mauro,T.M. and
            Elias,P.M.
  TITLE     Unbound Corneocyte Lipid Envelopes in 12R-Lipoxygenase Deficiency
            Support a Specific Role in Lipid-Protein Cross-Linking
  JOURNAL   Am J Pathol 191 (5), 921-929 (2021)
   PUBMED   33607042
  REMARK    GeneRIF: Unbound Corneocyte Lipid Envelopes in 12R-Lipoxygenase
            Deficiency Support a Specific Role in Lipid-Protein Cross-Linking.
REFERENCE   3  (bases 1 to 2347)
  AUTHORS   Leiria,L.O., Wang,C.H., Lynes,M.D., Yang,K., Shamsi,F., Sato,M.,
            Sugimoto,S., Chen,E.Y., Bussberg,V., Narain,N.R., Sansbury,B.E.,
            Darcy,J., Huang,T.L., Kodani,S.D., Sakaguchi,M., Rocha,A.L.,
            Schulz,T.J., Bartelt,A., Hotamisligil,G.S., Hirshman,M.F., van
            Leyen,K., Goodyear,L.J., Bluher,M., Cypess,A.M., Kiebish,M.A.,
            Spite,M. and Tseng,Y.H.
  TITLE     12-Lipoxygenase Regulates Cold Adaptation and Glucose Metabolism by
            Producing the Omega-3 Lipid 12-HEPE from Brown Fat
  JOURNAL   Cell Metab 30 (4), 768-783 (2019)
   PUBMED   31353262
REFERENCE   4  (bases 1 to 2347)
  AUTHORS   Pichery,M., Huchenq,A., Sandhoff,R., Severino-Freire,M.,
            Zaafouri,S., Opalka,L., Levade,T., Soldan,V., Bertrand-Michel,J.,
            Lhuillier,E., Serre,G., Maruani,A., Mazereeuw-Hautier,J. and
            Jonca,N.
  TITLE     PNPLA1 defects in patients with autosomal recessive congenital
            ichthyosis and KO mice sustain PNPLA1 irreplaceable function in
            epidermal omega-O-acylceramide synthesis and skin permeability
            barrier
  JOURNAL   Hum Mol Genet 26 (10), 1787-1800 (2017)
   PUBMED   28369476
REFERENCE   5  (bases 1 to 2347)
  AUTHORS   Krieg,P. and Furstenberger,G.
  TITLE     The role of lipoxygenases in epidermis
  JOURNAL   Biochim Biophys Acta 1841 (3), 390-400 (2014)
   PUBMED   23954555
  REMARK    GeneRIF: Loss-of-function mutations in the LOX genes ALOX12B and
            ALOXE3 have been found to represent the second most common cause of
            autosomal recessive congenital ichthyosis. [review]
            Review article
            Erratum:[Biochim Biophys Acta. 2014 Dec;1841(12):1767]
REFERENCE   6  (bases 1 to 2347)
  AUTHORS   Siebert,M., Krieg,P., Lehmann,W.D., Marks,F. and Furstenberger,G.
  TITLE     Enzymic characterization of epidermis-derived 12-lipoxygenase
            isoenzymes
  JOURNAL   Biochem J 355 (Pt 1), 97-104 (2001)
   PUBMED   11256953
REFERENCE   7  (bases 1 to 2347)
  AUTHORS   Kinzig,A., Heidt,M., Furstenberger,G., Marks,F. and Krieg,P.
  TITLE     cDNA cloning, genomic structure, and chromosomal localization of a
            novel murine epidermis-type lipoxygenase
  JOURNAL   Genomics 58 (2), 158-164 (1999)
   PUBMED   10366447
REFERENCE   8  (bases 1 to 2347)
  AUTHORS   Krieg,P., Siebert,M., Kinzig,A., Bettenhausen,R., Marks,F. and
            Furstenberger,G.
  TITLE     Murine 12(R)-lipoxygenase: functional expression, genomic structure
            and chromosomal localization
  JOURNAL   FEBS Lett 446 (1), 142-148 (1999)
   PUBMED   10100631
REFERENCE   9  (bases 1 to 2347)
  AUTHORS   Sun,D., McDonnell,M., Chen,X.S., Lakkis,M.M., Li,H., Isaacs,S.N.,
            Elsea,S.H., Patel,P.I. and Funk,C.D.
  TITLE     Human 12(R)-lipoxygenase and the mouse ortholog. Molecular cloning,
            expression, and gene chromosomal assignment
  JOURNAL   J Biol Chem 273 (50), 33540-33547 (1998)
   PUBMED   9837935
REFERENCE   10 (bases 1 to 2347)
  AUTHORS   Krieg,P., Kinzig,A., Heidt,M., Marks,F. and Furstenberger,G.
  TITLE     cDNA cloning of a 8-lipoxygenase and a novel epidermis-type
            lipoxygenase from phorbol ester-treated mouse skin
  JOURNAL   Biochim Biophys Acta 1391 (1), 7-12 (1998)
   PUBMED   9518531
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL645527.20.
            
            On Nov 17, 2006 this sequence version replaced NM_009659.1.
            
            Summary: This gene encodes an enzyme involved in the conversion of
            arachidonic acid to 12R-hydroxyeicosatetraenoic acid. Mutations in
            this gene can prevent the formation of the epidermal permeability
            barrier and cause an ichthyosiform phenotype. [provided by RefSeq,
            Sep 2015].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: Y14334.1, BC113149.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849376, SAMN00849387
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-322               AL645527.20        73635-73956
            323-527             AL645527.20        74837-75041
            528-609             AL645527.20        79231-79312
            610-702             AL645527.20        79456-79548
            703-825             AL645527.20        79684-79806
            826-929             AL645527.20        80071-80174
            930-1102            AL645527.20        80490-80662
            1103-1246           AL645527.20        80895-81038
            1247-1450           AL645527.20        82414-82617
            1451-1537           AL645527.20        82793-82879
            1538-1707           AL645527.20        83337-83506
            1708-1829           AL645527.20        83919-84040
            1830-1930           AL645527.20        85341-85441
            1931-2101           AL645527.20        85781-85951
            2102-2347           AL645527.20        86109-86354
FEATURES             Location/Qualifiers
     source          1..2347
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 42.38 cM"
     gene            1..2347
                     /gene="Alox12b"
                     /gene_synonym="12R-LOX; Aloxe2; e-LOX2"
                     /note="arachidonate 12-lipoxygenase, 12R type"
                     /db_xref="GeneID:11686"
                     /db_xref="MGI:MGI:1274782"
     exon            1..322
                     /gene="Alox12b"
                     /gene_synonym="12R-LOX; Aloxe2; e-LOX2"
                     /inference="alignment:Splign:2.1.0"
     CDS             176..2281
                     /gene="Alox12b"
                     /gene_synonym="12R-LOX; Aloxe2; e-LOX2"
                     /EC_number="1.13.11.-"
                     /note="12R-lipoxygenase; epidermis-type lipoxygenase 2;
                     epidermis-type lipoxygenase 12"
                     /codon_start=1
                     /product="arachidonate 12-lipoxygenase, 12R-type"
                     /protein_id="NP_033789.1"
                     /db_xref="CCDS:CCDS24885.1"
                     /db_xref="GeneID:11686"
                     /db_xref="MGI:MGI:1274782"
                     /translation="MATYKVKVATGTDFFSGTLDSISLTIVGTQGESHKQRLNHFGRD
                     FATGAVDDYTVQCQQDLGELIIIRLHKEPHSFLAKDPWYCNYVQICAPDCRVYHFPAY
                     QWMDGYETLALREATGKITADDTLPILLEHRQEEIRAKKDFYHWRVFVPGLPNYVDIP
                     SYHPPPRRCRNPNRPEWDGYIPGFPILINIKATRFLNSNLRFSFVKTASFFYRLGPMA
                     LAFKLRGLVDRKRSWKRLKDIKNIFPATKSVVSEYVAEHWTEDSFFGYQYLNGINPGL
                     IRRCTQIPDKFPVTDEMVAPFLGEGTCLQAELERGNIYLADYRILDGIPTVELNGQQQ
                     HHCAPMCLLHFGPDGNMMPIAIQLSQTPGPDCPIFLPNDSEWDWLLAKTWVRYAEFYS
                     HEAVAHLLESHLIGEAFCLALLRNLPMCHPLYKLLIPHTRYNVQINSIGRALLLNKGG
                     LSARAMSLGLEGFAQVMVRGLSELTYKSLCIPNDFVERGVQDLPGYYFRDDSLAVWYA
                     MERYVTEIITYYYPNDAAVEGDPELQCWVQEIFKECLLGRESSGFPTCLRTIPELIEY
                     VTMVMYTCSARHAAVNSGQLEYTSWMPNFPSSMRNPPMQTKGLTTLQTYMDTLPDVKT
                     TCIVLLVLWTLCREPDDRRPLGHFPDIHFVEEGPRRSIEAFRQNLNQISHNIRQRNKC
                     LTLPYYYLDPVLIENSISI"
     exon            323..527
                     /gene="Alox12b"
                     /gene_synonym="12R-LOX; Aloxe2; e-LOX2"
                     /inference="alignment:Splign:2.1.0"
     exon            528..609
                     /gene="Alox12b"
                     /gene_synonym="12R-LOX; Aloxe2; e-LOX2"
                     /inference="alignment:Splign:2.1.0"
     exon            610..702
                     /gene="Alox12b"
                     /gene_synonym="12R-LOX; Aloxe2; e-LOX2"
                     /inference="alignment:Splign:2.1.0"
     exon            703..825
                     /gene="Alox12b"
                     /gene_synonym="12R-LOX; Aloxe2; e-LOX2"
                     /inference="alignment:Splign:2.1.0"
     exon            826..929
                     /gene="Alox12b"
                     /gene_synonym="12R-LOX; Aloxe2; e-LOX2"
                     /inference="alignment:Splign:2.1.0"
     exon            930..1102
                     /gene="Alox12b"
                     /gene_synonym="12R-LOX; Aloxe2; e-LOX2"
                     /inference="alignment:Splign:2.1.0"
     exon            1103..1246
                     /gene="Alox12b"
                     /gene_synonym="12R-LOX; Aloxe2; e-LOX2"
                     /inference="alignment:Splign:2.1.0"
     exon            1247..1450
                     /gene="Alox12b"
                     /gene_synonym="12R-LOX; Aloxe2; e-LOX2"
                     /inference="alignment:Splign:2.1.0"
     exon            1451..1537
                     /gene="Alox12b"
                     /gene_synonym="12R-LOX; Aloxe2; e-LOX2"
                     /inference="alignment:Splign:2.1.0"
     exon            1538..1707
                     /gene="Alox12b"
                     /gene_synonym="12R-LOX; Aloxe2; e-LOX2"
                     /inference="alignment:Splign:2.1.0"
     exon            1708..1829
                     /gene="Alox12b"
                     /gene_synonym="12R-LOX; Aloxe2; e-LOX2"
                     /inference="alignment:Splign:2.1.0"
     exon            1830..1930
                     /gene="Alox12b"
                     /gene_synonym="12R-LOX; Aloxe2; e-LOX2"
                     /inference="alignment:Splign:2.1.0"
     exon            1931..2101
                     /gene="Alox12b"
                     /gene_synonym="12R-LOX; Aloxe2; e-LOX2"
                     /inference="alignment:Splign:2.1.0"
     exon            2102..2347
                     /gene="Alox12b"
                     /gene_synonym="12R-LOX; Aloxe2; e-LOX2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2326..2331
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Alox12b"
                     /gene_synonym="12R-LOX; Aloxe2; e-LOX2"
                     /note="hexamer: AATAAA"
     polyA_site      2347
                     /gene="Alox12b"
                     /gene_synonym="12R-LOX; Aloxe2; e-LOX2"
                     /note="major polyA site"
ORIGIN      
        1 agctgctccc ggctccagag gcattcgcgg gacagtggtt cccgctgact gctccatcac
       61 tgactgctcc atcactcttc gctgtctggc ttcccttgtc tacctcgttt ctaactgtgt
      121 ggctttagcc atagtgtccg ggattgagcc ctctcttggc tcctgaagag cggccatggc
      181 cacctataaa gtcaaggtgg ccacgggcac tgacttcttt tcggggacgc tggattcaat
      241 ttcactgacc atcgtgggga cccaaggaga gagccataag cagcggttga accactttgg
      301 gagagacttt gccacggggg cggtggatga ctacacggta cagtgccagc aggacctggg
      361 ggagcttatc atcatccgtc tgcacaagga gcctcattcc ttcctagcca aggacccctg
      421 gtactgcaac tatgtgcaga tctgtgctcc cgactgccgt gtctaccact tcccagctta
      481 ccagtggatg gatggctatg agaccctggc actccgggag gccacaggaa agataacagc
      541 agacgacaca ctccctatcc ttttggaaca cagacaagaa gagatcagag ccaagaagga
      601 cttctaccac tggagggtct ttgttcctgg ccttcccaac tatgtggata tccccagtta
      661 ccatcctcct ccccgacggt gccgcaatcc caaccggcct gagtgggatg gttatattcc
      721 cggattccca attctcatca acattaaggc taccaggttc ttaaactcaa atcttcgttt
      781 ctccttcgtc aagacagcct ccttcttcta ccgcctaggg cccatggcgc tggccttcaa
      841 actgcgtggc ctggtggatc gaaaacgctc ctggaagaga ctaaaggaca ttaagaatat
      901 tttccctgct accaagtctg tcgtctcaga gtatgtggcc gagcactgga cagaggacag
      961 cttctttggg taccagtacc ttaatggtat taacccaggc ctgatccgaa gatgtacaca
     1021 gatcccagac aaattcccag tcacagatga aatggtggcc ccattcctgg gtgaaggaac
     1081 atgcctgcaa gcagagctgg agagggggaa tatttacctg gcagactacc gcatcctgga
     1141 tggcatcccc accgtggagc tcaatggtca gcagcagcat cactgtgccc cgatgtgctt
     1201 gctgcacttt ggtcctgatg gcaacatgat gcccatcgcc attcagctca gtcagactcc
     1261 tgggcctgat tgtcctatct tcctgccgaa tgattctgag tgggactggc tgttggctaa
     1321 aacgtgggtg cgctacgcgg agttctacag ccacgaggcc gtggcacact tgctggagag
     1381 ccacctcatc ggggaagctt tctgcttggc cctcctgagg aacctgccca tgtgccaccc
     1441 cctgtataag cttctcatcc ctcacactcg gtacaacgtc cagatcaata gcattgggcg
     1501 ggccctcctt ctcaacaagg gaggtctctc tgccagggcc atgtccctgg gcctagaggg
     1561 cttcgcacag gtgatggttc ggggtctgtc tgagctcacc tacaaaagcc tctgcattcc
     1621 caacgacttt gtggagcgcg gggtccagga cctgcctggg tattatttcc gtgatgacag
     1681 cctggcggtg tggtacgcga tggagaggta cgtgacagag atcatcactt attattaccc
     1741 aaatgacgca gccgtagagg gcgacccgga actgcagtgc tgggtacagg agatattcaa
     1801 ggagtgtctc ctggggcgtg agagctcagg cttccccacc tgcttgcgaa ctatacctga
     1861 gctgatcgaa tacgtcacaa tggtcatgta cacctgctcg gccaggcatg cagctgtcaa
     1921 ctcaggccag ctggagtaca cctcttggat gcccaacttc ccatcatcca tgaggaaccc
     1981 accgatgcag accaaggggc taactactct gcagacatac atggacactc ttccggacgt
     2041 gaagaccaca tgcattgtgc tcttggtgct ctggactctc tgtcgggagc ctgatgacag
     2101 gcggccgctt ggccacttcc cagacatcca ttttgtggag gagggtcctc ggaggagtat
     2161 tgaggctttt cgtcagaacc tcaaccagat ctctcacaac atccgacagc gcaataagtg
     2221 cctcaccctc ccgtactact acctggaccc agtgctgatt gagaacagta tttccatcta
     2281 gtccaccctt ctgccctccc ctccatacac tgcctcctat tttccaataa aatcactgta
     2341 aacattg
//
