LOCUS       NM_001405036            2694 bp    mRNA    linear   ROD 08-DEC-2024
DEFINITION  Mus musculus alkB homolog 8, tRNA methyltransferase (Alkbh8),
            transcript variant 1, mRNA.
ACCESSION   NM_001405036 XM_006509879
VERSION     NM_001405036.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2694)
  AUTHORS   Nakai,M., Hase,H., Zhao,Y., Okawa,K., Honda,K., Ikuma,K., Kitae,K.
            and Tsujikawa,K.
  TITLE     RNA-modifying enzyme Alkbh8 is involved in mouse embryonic
            development
  JOURNAL   iScience 27 (9), 110777 (2024)
   PUBMED   39280612
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2694)
  AUTHORS   Honda,K., Hase,H., Tanikawa,S., Okawa,K., Chen,L., Yamaguchi,T.,
            Nakai,M., Kitae,K., Ago,Y., Nakagawa,S. and Tsujikawa,K.
  TITLE     ALKBH8 contributes to neurological function through oxidative
            stress regulation
  JOURNAL   PNAS Nexus 3 (3), pgae115 (2024)
   PUBMED   38550277
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2694)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   4  (bases 1 to 2694)
  AUTHORS   Leonardi,A., Kovalchuk,N., Yin,L., Endres,L., Evke,S., Nevins,S.,
            Martin,S., Dedon,P.C., Melendez,J.A., Van Winkle,L., Zhang,Q.Y.,
            Ding,X. and Begley,T.J.
  TITLE     The epitranscriptomic writer ALKBH8 drives tolerance and protects
            mouse lungs from the environmental pollutant naphthalene
  JOURNAL   Epigenetics 15 (10), 1121-1138 (2020)
   PUBMED   32303148
  REMARK    GeneRIF: The epitranscriptomic writer ALKBH8 drives tolerance and
            protects mouse lungs from the environmental pollutant naphthalene.
REFERENCE   5  (bases 1 to 2694)
  AUTHORS   Lee,M.Y., Leonardi,A., Begley,T.J. and Melendez,J.A.
  TITLE     Loss of epitranscriptomic control of selenocysteine utilization
            engages senescence and mitochondrial reprogramming
  JOURNAL   Redox Biol 28, 101375 (2020)
   PUBMED   31765888
  REMARK    GeneRIF: Loss of epitranscriptomic control of selenocysteine
            utilization engages senescence and mitochondrial reprogramming().
REFERENCE   6  (bases 1 to 2694)
  AUTHORS   Songe-Moller,L., van den Born,E., Leihne,V., Vagbo,C.B.,
            Kristoffersen,T., Krokan,H.E., Kirpekar,F., Falnes,P.O. and
            Klungland,A.
  TITLE     Mammalian ALKBH8 possesses tRNA methyltransferase activity required
            for the biogenesis of multiple wobble uridine modifications
            implicated in translational decoding
  JOURNAL   Mol Cell Biol 30 (7), 1814-1827 (2010)
   PUBMED   20123966
  REMARK    GeneRIF: Data show that ALKBH8 is a tRNA methyltransferase required
            for the final step in the biogenesis of mcm5U.
            GeneRIF: The methyltransferase domain of ALKBH8 is demonstrated to
            be a functional homologue of the Saccharomyces cerevisiae Trm9
            protein, mediating the last step in the formation of the wobble
            uridine modification 5-methoxycarbonylmethyl-uridine in tRNA. This
            modification is shown to be important for efficient selenoprotein
            synthesis. ALKBH8 knock-out mice are generated and described.
REFERENCE   7  (bases 1 to 2694)
  AUTHORS   Lee,D.H., Jin,S.G., Cai,S., Chen,Y., Pfeifer,G.P. and O'Connor,T.R.
  TITLE     Repair of methylation damage in DNA and RNA by mammalian AlkB
            homologues
  JOURNAL   J Biol Chem 280 (47), 39448-39459 (2005)
   PUBMED   16174769
REFERENCE   8  (bases 1 to 2694)
  AUTHORS   McKee,A.E., Minet,E., Stern,C., Riahi,S., Stiles,C.D. and
            Silver,P.A.
  TITLE     A genome-wide in situ hybridization map of RNA-binding proteins
            reveals anatomically restricted expression in the developing mouse
            brain
  JOURNAL   BMC Dev Biol 5, 14 (2005)
   PUBMED   16033648
  REMARK    Publication Status: Online-Only
REFERENCE   9  (bases 1 to 2694)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   10 (bases 1 to 2694)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC162921.3.
            
            On Apr 19, 2022 this sequence version replaced XM_006509879.5.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK133489.1, SRR1660815.121555.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849381, SAMN00849390
                                           [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-444               AC162921.3         152546-152989
            445-580             AC162921.3         155851-155986
            581-818             AC162921.3         160410-160647
            819-950             AC162921.3         161983-162114
            951-1046            AC162921.3         163176-163271
            1047-1151           AC162921.3         165199-165303
            1152-1222           AC162921.3         166815-166885
            1223-1329           AC162921.3         176879-176985
            1330-1481           AC162921.3         185259-185410
            1482-1738           AC162921.3         187053-187309
            1739-1888           AC162921.3         200089-200238
            1889-2694           AC162921.3         202437-203242
FEATURES             Location/Qualifiers
     source          1..2694
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="9"
                     /map="9 2.02 cM"
     gene            1..2694
                     /gene="Alkbh8"
                     /gene_synonym="4930562C03Rik; 8030431D03Rik;
                     9430088N01Rik; Abh8"
                     /note="alkB homolog 8, tRNA methyltransferase"
                     /db_xref="GeneID:67667"
                     /db_xref="MGI:MGI:1914917"
     exon            1..444
                     /gene="Alkbh8"
                     /gene_synonym="4930562C03Rik; 8030431D03Rik;
                     9430088N01Rik; Abh8"
                     /inference="alignment:Splign:2.1.0"
     CDS             317..2446
                     /gene="Alkbh8"
                     /gene_synonym="4930562C03Rik; 8030431D03Rik;
                     9430088N01Rik; Abh8"
                     /EC_number="2.1.1.229"
                     /note="isoform 1 is encoded by transcript variant 1;
                     alkylated DNA repair protein alkB homolog 8; alkB,
                     alkylation repair homolog 8; probable
                     alpha-ketoglutarate-dependent dioxygenase ABH8;
                     S-adenosyl-L-methionine-dependent tRNA methyltransferase
                     ABH8; tRNA
                     (carboxymethyluridine(34)-5-O)-methyltransferase ABH8;
                     alpha-ketoglutarate-dependent dioxygenase ALKBH8;
                     S-adenosyl-L-methionine-dependent tRNA methyltransferase
                     ALKBH8; tRNA
                     (carboxymethyluridine(34)-5-O)-methyltransferase ALKBH8"
                     /codon_start=1
                     /product="tRNA
                     (carboxymethyluridine(34)-5-O)-methyltransferase ALKBH8
                     isoform 1"
                     /protein_id="NP_001391965.1"
                     /db_xref="GeneID:67667"
                     /db_xref="MGI:MGI:1914917"
                     /translation="MGNVVLLSATWRRPVERVSEHRWSGCTGEGWRLTKQLGGEHFCI
                     TMNINHKGVLKLTKMEKKFLRKQSKARHVLLKHEGIQAVSYPTQSLVIANGGLGNGVS
                     RKQLLLTLEKCGPVEALLMPPNKPYAFVIFQTIEESKKAYFTLNGKEIIDDLGQKIFL
                     YLNFVEKAQWKNMGLEALPPGLLVVEEIISSEEEKKLLESVNWTEDTGNQNFQRSLKH
                     RRVKHFGYEFHYESNTVDKDKPLPGGLPEVCSSILEKLLKEGYIKHKPDQLTINQYEP
                     GHGIPAHIDTHSAFEDEIISLSLGSAIVMDFKHPEGVTVQVMLPRRSLLVMTGESRYL
                     WTHGITPRKFDTVQASEQFKGGIITSDIGDLTLSKRGMRTSFTFRKVRRMPCNCSYSS
                     VCDRQRKATPPSLTESSKEALELEQKHVHQVYNEIASHFSSTRHSPWPRIVEFLKALP
                     SGSIVADIGCGNGKYLGINKDLYMIGCDRSQNLVDICRERQFQALVCDALAVPVRSGS
                     CDACISIAVIHHFATAERRVEALQELARLLRPGGQALIYVWAMEQEYKNQKSKYLRGK
                     RISQGDKDELNSATSTEEFLVNQTPEGVNEDPALSVNSSSITKEEEYKSRKVPNSELP
                     IHINRTCFHSQDVLVPWHLKRNPGKDKAIEPSGVAGCPDPSPVFHRYYHVFCDGELEA
                     SCQAVGDVSILQSYYDQGNWCVVLQKV"
     exon            445..580
                     /gene="Alkbh8"
                     /gene_synonym="4930562C03Rik; 8030431D03Rik;
                     9430088N01Rik; Abh8"
                     /inference="alignment:Splign:2.1.0"
     exon            581..818
                     /gene="Alkbh8"
                     /gene_synonym="4930562C03Rik; 8030431D03Rik;
                     9430088N01Rik; Abh8"
                     /inference="alignment:Splign:2.1.0"
     exon            819..950
                     /gene="Alkbh8"
                     /gene_synonym="4930562C03Rik; 8030431D03Rik;
                     9430088N01Rik; Abh8"
                     /inference="alignment:Splign:2.1.0"
     exon            951..1046
                     /gene="Alkbh8"
                     /gene_synonym="4930562C03Rik; 8030431D03Rik;
                     9430088N01Rik; Abh8"
                     /inference="alignment:Splign:2.1.0"
     exon            1047..1151
                     /gene="Alkbh8"
                     /gene_synonym="4930562C03Rik; 8030431D03Rik;
                     9430088N01Rik; Abh8"
                     /inference="alignment:Splign:2.1.0"
     exon            1152..1222
                     /gene="Alkbh8"
                     /gene_synonym="4930562C03Rik; 8030431D03Rik;
                     9430088N01Rik; Abh8"
                     /inference="alignment:Splign:2.1.0"
     exon            1223..1329
                     /gene="Alkbh8"
                     /gene_synonym="4930562C03Rik; 8030431D03Rik;
                     9430088N01Rik; Abh8"
                     /inference="alignment:Splign:2.1.0"
     exon            1330..1481
                     /gene="Alkbh8"
                     /gene_synonym="4930562C03Rik; 8030431D03Rik;
                     9430088N01Rik; Abh8"
                     /inference="alignment:Splign:2.1.0"
     exon            1482..1738
                     /gene="Alkbh8"
                     /gene_synonym="4930562C03Rik; 8030431D03Rik;
                     9430088N01Rik; Abh8"
                     /inference="alignment:Splign:2.1.0"
     exon            1739..1888
                     /gene="Alkbh8"
                     /gene_synonym="4930562C03Rik; 8030431D03Rik;
                     9430088N01Rik; Abh8"
                     /inference="alignment:Splign:2.1.0"
     exon            1889..2694
                     /gene="Alkbh8"
                     /gene_synonym="4930562C03Rik; 8030431D03Rik;
                     9430088N01Rik; Abh8"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      2694
                     /gene="Alkbh8"
                     /gene_synonym="4930562C03Rik; 8030431D03Rik;
                     9430088N01Rik; Abh8"
                     /note="major polyA site"
ORIGIN      
        1 ctctgcagtg ttctaagcct gaggtatagg agttgtgctg tagatgtcag ttgagactga
       61 actctacagc tctgcatttc gattggtgtg gtttacacag gtaagttccc ttaagactac
      121 agttatttgt gagtataaga tcaaatgttt agcatttagt aacgtgatag ttgtaggtcc
      181 tattccaacg attactattc acccaaataa agcagccaaa ttttctgttt caagcagcag
      241 tttacagaca tttggcggag aaagggtcaa caaagaattt gagtcccgaa gggggttgtg
      301 gtagccccag ggactcatgg gaaatgtagt actattgtct gcaacatggc ggcgtccggt
      361 tgaaagggtg tcagagcata ggtggtcagg ctgcaccggg gaaggctggc gattgacaaa
      421 gcagctggga ggtgaacatt tctgtattac tatgaatatc aatcataaag gtgttttgaa
      481 actcactaaa atggaaaaga agttcttaag gaaacagagt aaagccaggc atgtgttgct
      541 gaaacatgaa ggcatccaag cagtgtctta tcccactcag agcctggtta ttgccaatgg
      601 tggtctgggt aatggcgtga gtaggaaaca actgcttttg actttggaga aatgtggacc
      661 tgtggaagct ctcctcatgc cacctaataa gccatatgca tttgtaatat tccaaactat
      721 tgaagaatcc aagaaagcat attttaccct caatggaaaa gaaatcattg atgatttggg
      781 gcagaagatc tttctgtact tgaattttgt tgaaaaagca caatggaaaa acatgggcct
      841 tgaagcctta cctccaggcc tcttggtggt agaagaaatt atttcttctg aggaggagaa
      901 aaagcttctg gagagtgtga attggacaga agatacaggc aatcagaatt ttcaaaggtc
      961 cttaaaacac agaagagtga aacattttgg ctatgaattt cactatgaga gcaacactgt
     1021 ggataaagac aagcccttac ctgggggtct tcctgaggtt tgcagtagca ttttggagaa
     1081 gttgttgaaa gaaggttata ttaaacataa accagaccag ttgaccataa atcaatatga
     1141 acctgggcat ggaattcctg cccatattga cacgcattct gcatttgaag atgagattat
     1201 ttctctcagt ttggggtcag cgattgttat ggatttcaag catccagagg gtgtcacagt
     1261 acaagttatg ttacctcgtc ggagtttgct ggtgatgaca ggagaatcta gatacctttg
     1321 gactcatgga attacacctc gaaaatttga taccgtccaa gcgtctgagc aatttaaagg
     1381 gggaataatc accagtgaca ttggagactt gactttaagc aagaggggaa tgcgcacatc
     1441 ctttacgttt agaaaagtga ggcgaatgcc ctgcaattgt agttactcct cagtctgcga
     1501 cagacaaagg aaggccacac ctccatccct tacagagagc agtaaagaag cattggagtt
     1561 agagcaaaag catgtccatc aagtgtacaa tgagattgca agtcacttca gcagcacaag
     1621 acatagccca tggccacgca tagtagagtt cctgaaagct ttgccaagtg gttccatagt
     1681 ggcagatatc ggatgtggaa atggaaaata ccttggcatc aacaaggact tatatatgat
     1741 tgggtgtgat cgtagccaga accttgtgga catttgtaga gagaggcagt tccaggcctt
     1801 agtctgcgat gcgctggctg ttcctgtcag aagtgggtct tgtgatgctt gcatctctat
     1861 tgccgtcatt caccactttg caactgcaga gcgcagagtg gaagctctcc aagaacttgc
     1921 ccgtctcctg agacctggtg ggcaggccct catttatgtc tgggcaatgg aacaagagta
     1981 taaaaatcag aagtccaagt atcttagagg aaagagaatt agccaaggag ataaagatga
     2041 gctaaacagt gccacatcta cggaagaatt tctagtaaat caaacgcctg agggggtcaa
     2101 tgaagaccca gcattgtctg tcaactccag tagtatcacc aaggaagaag agtataagtc
     2161 aaggaaagtt cctaattctg aactccctat tcatataaat aggacctgct ttcattccca
     2221 agatgtgctg gttccctggc acctcaaaag aaaccctggt aaagacaaag ctattgaacc
     2281 atctggtgta gcaggatgtc ctgacccaag tcctgtgttt catcgttatt accatgtttt
     2341 ctgtgatgga gaattggaag cttcatgcca ggctgtgggt gatgttagca ttctgcagag
     2401 ctattacgat caagggaatt ggtgtgttgt tcttcaaaag gtctgatcac ttatctgaac
     2461 atatcatgtg taaagagaaa aaaaaaagtc aatctctttt tgaaaaggaa actaaattat
     2521 ctctttaaat aaggaggaaa tttggaacag agatacttag gaaacatttt tctctattcc
     2581 cgttgtctga cttcataaac gtaggttcct cttacatgcc ttttaagtat ggtgtttgac
     2641 attaacttgt gaaaagtctg tgatgtttgt atatatatag catttgaaga taaa
//
