LOCUS       NM_001418684            1409 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus alkB homolog 3, alpha-ketoglutarate-dependent
            dioxygenase (Alkbh3), transcript variant 5, mRNA.
ACCESSION   NM_001418684
VERSION     NM_001418684.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1409)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1409)
  AUTHORS   Capriati,M., Hao,C., D'Cruz,S.C., Monfort,C., Chevrier,C.,
            Warembourg,C. and Smagulova,F.
  TITLE     Genome-wide analysis of sex-specific differences in the
            mother-child PELAGIE cohort exposed to organophosphate metabolites
  JOURNAL   Sci Rep 13 (1), 8003 (2023)
   PUBMED   37198424
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1409)
  AUTHORS   Chen,Z., Qi,M., Shen,B., Luo,G., Wu,Y., Li,J., Lu,Z., Zheng,Z.,
            Dai,Q. and Wang,H.
  TITLE     Transfer RNA demethylase ALKBH3 promotes cancer progression via
            induction of tRNA-derived small RNAs
  JOURNAL   Nucleic Acids Res 47 (5), 2533-2545 (2019)
   PUBMED   30541109
REFERENCE   4  (bases 1 to 1409)
  AUTHORS   You,C., Wang,P., Nay,S.L., Wang,J., Dai,X., O'Connor,T.R. and
            Wang,Y.
  TITLE     Roles of Aag, Alkbh2, and Alkbh3 in the Repair of Carboxymethylated
            and Ethylated Thymidine Lesions
  JOURNAL   ACS Chem Biol 11 (5), 1332-1338 (2016)
   PUBMED   26930515
  REMARK    GeneRIF: These results revealed that N3-ethylthymidine , but not
            other DNA lesions, could be repaired by Alkbh2 and Alkbh3 in
            mammalian cells.
REFERENCE   5  (bases 1 to 1409)
  AUTHORS   Calvo,J.A., Meira,L.B., Lee,C.Y., Moroski-Erkul,C.A.,
            Abolhassani,N., Taghizadeh,K., Eichinger,L.W., Muthupalani,S.,
            Nordstrand,L.M., Klungland,A. and Samson,L.D.
  TITLE     DNA repair is indispensable for survival after acute inflammation
  JOURNAL   J Clin Invest 122 (7), 2680-2689 (2012)
   PUBMED   22684101
  REMARK    GeneRIF: ALKBH2 and ALKBH3 provide cancer protection similar to
            that of the DNA glycosylase AAG and display apparent epistasis with
            Aag
REFERENCE   6  (bases 1 to 1409)
  AUTHORS   Nay,S.L., Lee,D.H., Bates,S.E. and O'Connor,T.R.
  TITLE     Alkbh2 protects against lethality and mutation in primary mouse
            embryonic fibroblasts
  JOURNAL   DNA Repair (Amst) 11 (5), 502-510 (2012)
   PUBMED   22429847
REFERENCE   7  (bases 1 to 1409)
  AUTHORS   Ringvoll,J., Moen,M.N., Nordstrand,L.M., Meira,L.B., Pang,B.,
            Bekkelund,A., Dedon,P.C., Bjelland,S., Samson,L.D., Falnes,P.O. and
            Klungland,A.
  TITLE     AlkB homologue 2-mediated repair of ethenoadenine lesions in
            mammalian DNA
  JOURNAL   Cancer Res 68 (11), 4142-4149 (2008)
   PUBMED   18519673
REFERENCE   8  (bases 1 to 1409)
  AUTHORS   Ringvoll,J., Nordstrand,L.M., Vagbo,C.B., Talstad,V., Reite,K.,
            Aas,P.A., Lauritzen,K.H., Liabakk,N.B., Bjork,A., Doughty,R.W.,
            Falnes,P.O., Krokan,H.E. and Klungland,A.
  TITLE     Repair deficient mice reveal mABH2 as the primary oxidative
            demethylase for repairing 1meA and 3meC lesions in DNA
  JOURNAL   EMBO J 25 (10), 2189-2198 (2006)
   PUBMED   16642038
REFERENCE   9  (bases 1 to 1409)
  AUTHORS   Lee,D.H., Jin,S.G., Cai,S., Chen,Y., Pfeifer,G.P. and O'Connor,T.R.
  TITLE     Repair of methylation damage in DNA and RNA by mammalian AlkB
            homologues
  JOURNAL   J Biol Chem 280 (47), 39448-39459 (2005)
   PUBMED   16174769
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL772372.4.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR17253012.10136837.1,
                                           SRR17253011.6555550.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849381, SAMN01164131
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-72                AL772372.4         82888-82959         c
            73-226              AL772372.4         82460-82613         c
            227-376             AL772372.4         80648-80797         c
            377-480             AL772372.4         80244-80347         c
            481-515             AL772372.4         79052-79086         c
            516-563             AL772372.4         76938-76985         c
            564-667             AL772372.4         75258-75361         c
            668-756             AL772372.4         73630-73718         c
            757-966             AL772372.4         68388-68597         c
            967-1065            AL772372.4         53721-53819         c
            1066-1409           AL772372.4         52839-53182         c
FEATURES             Location/Qualifiers
     source          1..1409
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 51.62 cM"
     gene            1..1409
                     /gene="Alkbh3"
                     /gene_synonym="1700108H04Rik; 1810020C19Rik; Abh3; mABH3"
                     /note="alkB homolog 3, alpha-ketoglutarate-dependent
                     dioxygenase"
                     /db_xref="GeneID:69113"
                     /db_xref="MGI:MGI:1916363"
     exon            1..72
                     /gene="Alkbh3"
                     /gene_synonym="1700108H04Rik; 1810020C19Rik; Abh3; mABH3"
                     /inference="alignment:Splign:2.1.0"
     exon            73..226
                     /gene="Alkbh3"
                     /gene_synonym="1700108H04Rik; 1810020C19Rik; Abh3; mABH3"
                     /inference="alignment:Splign:2.1.0"
     exon            227..376
                     /gene="Alkbh3"
                     /gene_synonym="1700108H04Rik; 1810020C19Rik; Abh3; mABH3"
                     /inference="alignment:Splign:2.1.0"
     CDS             298..1158
                     /gene="Alkbh3"
                     /gene_synonym="1700108H04Rik; 1810020C19Rik; Abh3; mABH3"
                     /EC_number="1.14.11.33"
                     /EC_number="1.14.11.54"
                     /note="isoform a is encoded by transcript variant 5;
                     alpha-ketoglutarate-dependent dioxygenase alkB homolog 3;
                     alkylated DNA repair protein alkB homolog 3; alkB,
                     alkylation repair homolog 3"
                     /codon_start=1
                     /product="alpha-ketoglutarate-dependent dioxygenase alkB
                     homolog 3 isoform a"
                     /protein_id="NP_001405613.1"
                     /db_xref="GeneID:69113"
                     /db_xref="MGI:MGI:1916363"
                     /translation="MEDKRQRARVQGGWATPTKSQSATQPASPARSRLSQTAGPAWRS
                     KEQQQCDRQFVFKEPQLVVRAAPEPRVIDREGVYEISLSPTGVSRVCLYPGFVDLKEA
                     DWILEQLCKDVPWKQRMGIREDVTYPQPRLTAWYGELPYTYSRITMEPNPHWLPVLWT
                     LKSRIEENTSHTFNSLLCNFYRDEKDSVDWHSDDEPSLGSCPVIASLSFGATRTFEMR
                     KKPPPEENGDYTYVERVKIPLDHGTLLIMEGATQADWQHRVPKEYHSRQPRVNLTFRT
                     VYPDPRGAPR"
     exon            377..480
                     /gene="Alkbh3"
                     /gene_synonym="1700108H04Rik; 1810020C19Rik; Abh3; mABH3"
                     /inference="alignment:Splign:2.1.0"
     exon            481..515
                     /gene="Alkbh3"
                     /gene_synonym="1700108H04Rik; 1810020C19Rik; Abh3; mABH3"
                     /inference="alignment:Splign:2.1.0"
     exon            516..563
                     /gene="Alkbh3"
                     /gene_synonym="1700108H04Rik; 1810020C19Rik; Abh3; mABH3"
                     /inference="alignment:Splign:2.1.0"
     exon            564..667
                     /gene="Alkbh3"
                     /gene_synonym="1700108H04Rik; 1810020C19Rik; Abh3; mABH3"
                     /inference="alignment:Splign:2.1.0"
     exon            668..756
                     /gene="Alkbh3"
                     /gene_synonym="1700108H04Rik; 1810020C19Rik; Abh3; mABH3"
                     /inference="alignment:Splign:2.1.0"
     exon            757..966
                     /gene="Alkbh3"
                     /gene_synonym="1700108H04Rik; 1810020C19Rik; Abh3; mABH3"
                     /inference="alignment:Splign:2.1.0"
     exon            967..1065
                     /gene="Alkbh3"
                     /gene_synonym="1700108H04Rik; 1810020C19Rik; Abh3; mABH3"
                     /inference="alignment:Splign:2.1.0"
     exon            1066..1409
                     /gene="Alkbh3"
                     /gene_synonym="1700108H04Rik; 1810020C19Rik; Abh3; mABH3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1382..1387
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Alkbh3"
                     /gene_synonym="1700108H04Rik; 1810020C19Rik; Abh3; mABH3"
                     /note="hexamer: ATTAAA"
     polyA_site      1409
                     /gene="Alkbh3"
                     /gene_synonym="1700108H04Rik; 1810020C19Rik; Abh3; mABH3"
                     /note="major polyA site"
ORIGIN      
        1 actctgaagg ttccccggcg tccagacggc tgattagggt cgtgtcggga cctctgctca
       61 gggcccagcc aggttttcag gcttggagtt ccagggtggc gggacctttg ttaggaccta
      121 ttaagttctg ggactcctcg aactgacagc actcaggaag tctcagacag cactctggaa
      181 tttctgttca ccacttgcct taaacaagtc acgtcaaggt gagcaggtag ccatcccttg
      241 gtctgaatgg ggaatgaaat cttctgacat ctcagaacag aacgcttttt cttcaccatg
      301 gaggacaaga gacagcgagc ccgagtgcag ggaggctggg ctacacctac caagagccag
      361 tctgctactc agccagcttc ccctgctagg agccgtcttt cccagaccgc ggggccagcc
      421 tggaggagca aggaacagca gcaatgtgac cgacaatttg tgttcaaaga acctcagctg
      481 gttgtccgtg cagccccaga acccagagtg attgacaggg agggtgtgta tgaaatcagc
      541 ctgtcaccta ctggtgtgtc tagggtgtgc ttatatcctg gctttgtgga cttgaaggaa
      601 gctgactgga tcttggagca gctttgtaag gatgtcccct ggaaacagag gatgggcatc
      661 agagaggatg taacttatcc gcaaccaaga cttacagcat ggtatggaga gcttccttac
      721 acttactcga gaatcactat ggaaccaaat cctcactggc ttcctgtgct gtggactctg
      781 aagagccgca ttgaagagaa caccagccac accttcaact ccttgctgtg taatttttac
      841 cgggatgaga aggacagtgt ggactggcac agcgacgatg aaccatccct ggggagctgc
      901 cccgtcattg cttccctcag ttttggtgcc actcggactt ttgagatgag gaagaagccc
      961 ccacctgaag aaaatggaga ctatacatat gtggagagag tgaagatacc attggatcac
     1021 gggaccttgc taatcatgga aggagccaca caagccgact ggcagcaccg agtgcccaag
     1081 gaataccact ccagacaacc gagagtaaac ttaacctttc ggaccgttta tccagaccca
     1141 agaggagccc ctcggtgaca cttctacaga gttaccactg aggggagggc tatgcaccta
     1201 acagcccctc cccatctccc gcaagcactt ccgtgatgtg gctgttcaga aagcgtggtg
     1261 tttgcttctc aggttctact cctcctgaat gagagctgcc agcacgtcac cttgtcaaca
     1321 tgtctactac aggaacactt actttgagac aaatctctaa atcaaggtta tcattaagca
     1381 aattaaaaat tgctctggct gtactcaca
//
