LOCUS       NM_019998               3062 bp    mRNA    linear   ROD 30-JUL-2024
DEFINITION  Mus musculus ALG2 alpha-1,3/1,6-mannosyltransferase (Alg2),
            transcript variant 1, mRNA.
ACCESSION   NM_019998
VERSION     NM_019998.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3062)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3062)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643. PMID: 29144450]
REFERENCE   3  (bases 1 to 3062)
  AUTHORS   Henzl,M.T., Frey,B.B. and Wolf,A.J.
  TITLE     ALG-2 divalent-ion affinity: Calorimetric analysis of the des23
            versions reveals high-affinity site for Mg(2)
  JOURNAL   Biophys Chem 209, 28-40 (2016)
   PUBMED   26705706
  REMARK    GeneRIF: the observation that both ALG-2 isoforms bind Mg2+ with
            high affinity is the most significant finding of this study
REFERENCE   4  (bases 1 to 3062)
  AUTHORS   Imamura,K., Maeda,S., Kawamura,I., Matsuyama,K., Shinohara,N.,
            Yahiro,Y., Nagano,S., Setoguchi,T., Yokouchi,M., Ishidou,Y. and
            Komiya,S.
  TITLE     Human immunodeficiency virus type 1 enhancer-binding protein 3 is
            essential for the expression of asparagine-linked glycosylation 2
            in the regulation of osteoblast and chondrocyte differentiation
  JOURNAL   J Biol Chem 289 (14), 9865-9879 (2014)
   PUBMED   24563464
  REMARK    GeneRIF: Thus, Alg2, as a downstream mediator of Hivep3, suppresses
            osteogenesis, whereas it promotes chondrogenesis.
REFERENCE   5  (bases 1 to 3062)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   6  (bases 1 to 3062)
  AUTHORS   Tang,T., Li,L., Tang,J., Li,Y., Lin,W.Y., Martin,F., Grant,D.,
            Solloway,M., Parker,L., Ye,W., Forrest,W., Ghilardi,N., Oravecz,T.,
            Platt,K.A., Rice,D.S., Hansen,G.M., Abuin,A., Eberhart,D.E.,
            Godowski,P., Holt,K.H., Peterson,A., Zambrowicz,B.P. and de
            Sauvage,F.J.
  TITLE     A mouse knockout library for secreted and transmembrane proteins
  JOURNAL   Nat Biotechnol 28 (7), 749-755 (2010)
   PUBMED   20562862
REFERENCE   7  (bases 1 to 3062)
  AUTHORS   Draeby,I., Woods,Y.L., la Cour,J.M., Mollerup,J., Bourdon,J.C. and
            Berchtold,M.W.
  TITLE     The calcium binding protein ALG-2 binds and stabilizes Scotin, a
            p53-inducible gene product localized at the endoplasmic reticulum
            membrane
  JOURNAL   Arch Biochem Biophys 467 (1), 87-94 (2007)
   PUBMED   17889823
  REMARK    GeneRIF: ALG-2 binding to Scotin is strictly calcium dependent,
            indicating a role of this interaction in calcium signaling pathways
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY129752.1, BC052411.1 and BG093758.1.
            
            On Apr 4, 2008 this sequence version replaced NM_019998.2.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC051951.1, AK004997.2 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-91                BY129752.1         2-92
            92-3018             BC052411.1         45-2971
            3019-3062           BG093758.1         1-44                c
FEATURES             Location/Qualifiers
     source          1..3062
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 26.03 cM"
     gene            1..3062
                     /gene="Alg2"
                     /gene_synonym="1110018A23Rik; 1300013N08Rik; ALPG2; CDGIi;
                     MNCb-5081"
                     /note="ALG2 alpha-1,3/1,6-mannosyltransferase"
                     /db_xref="GeneID:56737"
                     /db_xref="MGI:MGI:1914731"
     exon            1..429
                     /gene="Alg2"
                     /gene_synonym="1110018A23Rik; 1300013N08Rik; ALPG2; CDGIi;
                     MNCb-5081"
                     /inference="alignment:Splign:2.1.0"
     exon            430..3055
                     /gene="Alg2"
                     /gene_synonym="1110018A23Rik; 1300013N08Rik; ALPG2; CDGIi;
                     MNCb-5081"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2996..3001
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Alg2"
                     /gene_synonym="1110018A23Rik; 1300013N08Rik; ALPG2; CDGIi;
                     MNCb-5081"
                     /note="hexamer: ATTAAA"
     polyA_site      3018
                     /gene="Alg2"
                     /gene_synonym="1110018A23Rik; 1300013N08Rik; ALPG2; CDGIi;
                     MNCb-5081"
                     /note="major polyA site"
     regulatory      3036..3041
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Alg2"
                     /gene_synonym="1110018A23Rik; 1300013N08Rik; ALPG2; CDGIi;
                     MNCb-5081"
                     /note="hexamer: AATAAA"
     polyA_site      3055
                     /gene="Alg2"
                     /gene_synonym="1110018A23Rik; 1300013N08Rik; ALPG2; CDGIi;
                     MNCb-5081"
ORIGIN      
        1 agtagccaga gggggccagg ggcggagctt gcaggagctt gcagaagctc gcgcggagag
       61 cgcaagcgcg gctcatctac catggccgaa aacctgtacc gagcccgttc ccgggtttac
      121 agcccatctg tgctgtttct gcacccagac atgggtatag gcggagccga gcgcctagtg
      181 ctggacgcgg cgctggcgct gcaggagtac ggctgtgatg tgaagatatg gaccgcgcac
      241 tacgacccga accactgctt catcgagacc cgcgagctct cggtgcaatg cgcaggggac
      301 tggctgcctc gcagcctggg ctggggcggc cgcggcgccg ccatctgctc ctacgtgcgc
      361 atggtctttc tggcgctcta cgtgctgttt ctctccggcg aggagttcga cgtggtggtg
      421 tgcgaccagg tgtctgcctg tatcccggtg ttcaaactgg ccagacggcg taagagggtc
      481 ctattttact gtcacttccc agatctgctg cttactcaga gaaattcagc tctgaagaag
      541 ttctacaggg cccccatcga ctggatcgag gaatacacca caggcatggc agaccgcatc
      601 ttggtcaaca gccagtacac tgcttccgtc tttaaagaaa ccttcaagac cctgtctcac
      661 agaaatcctg atgtgctcta cccatctctg aatatcggca gctttgactt ggctattcct
      721 gaaaagatag atgacctcgt ccccaagggg aagcaattcc tgttcctctc tatcaaccga
      781 tacgaaagga agaaaaatct gcccttggca ctgagatcct tggtgcagct tcggaatcgg
      841 ttaccatctc aagagtggga taaggttcat ctcttcatgg ccggtggtta tgacgatagg
      901 atcccggaga acgtggagca ctataaggag ttgaagaaaa tggtccaaga gtcagacctt
      961 gagcgtcatg tgaccttcct gcggtccttc tcggacagac agaagatctc actcctccac
     1021 ggctgcttgt gtgtgctcta cactccgagc aacgagcact ttggcatcgt ccctctggag
     1081 gccatgtaca tgcagtgccc agtcatcgct gttaataacg gtgggcccct ggagtccatt
     1141 gtccacaagg tcacggggtt cctgtgtgag ccagacccag tgcacttctc agaagccatg
     1201 gagaagttca tccacaaacc atccttaaaa gccacgatgg gcctcgctgg aaaagccagg
     1261 gtggcggaga agttttcagc tgatgcattt gcagaccagc tgtaccagta tgtcacgaag
     1321 ctggtgtagt caggagagta cagtgtagag tctgtatgct acatgcactg cgatccctct
     1381 tatgggttgt agaaacgcct ttgaaaccaa aagaaagggg gaaacaaagg agctagaatc
     1441 tagtgctagt gagattttct tttttaagta gacttgagtc ttgaatgtga gccacctgtc
     1501 caccacacct acctgtcatt tctgaaatat tcttaatgct gtaatcattc cgtatctcgt
     1561 gagtgttgtt gaaagtatgg taggaatctg ctgctagcag actattttaa ctctattttc
     1621 ctggattatt gttcctttat atataaaggt ttgatcatcc agtgccttaa ttggctttag
     1681 tagtgtaggt cttaccattg tcacagtcaa ttgatttgac ttcagagcgt aatgggaacg
     1741 aggctgctgc agctcccaag ctcggtttac ttaaagtgtt ctctagtagc tgctagaaaa
     1801 tttttgttag aattttggct ggatctataa caagagtact cagtattatt tattgttttg
     1861 gtttttgttt tgttttgttt tttactgcaa cttgacaata aaatatgttt ggcatcagaa
     1921 gagaatagaa cattagatct gagcccgatg ctctgacttc atcttgttaa atacataaac
     1981 ctgaagtcag aagactagga taaatgagat agtttacatg aaggaacacc atcacacatg
     2041 ttttggccta ccgtgtcacg cttggcctta ttcctttgag cctgtgccag aacagtgtat
     2101 tgaggcaggg agcaaagaca aaaagggtcc catatccctt tctagaaaat atcccttgat
     2161 gacctgattc tcttccacta gccctcacta ctaaaagtcc tgtcacctcc tagtgtggct
     2221 ctcagtgtgg gccggggacc aggcctcagg gcacaggcct ttgggaactg tattagagtt
     2281 ctctaaaata gcaaacagca cttaacagaa tgaattccca gaagaagaag aacaaatggc
     2341 gtacaggctt ctgtccagct aatccaacaa tgaacagaaa gtccagaaat ccagtagttg
     2401 ttcagtccag gaggctgaat gtctcagctg atcttcagta tacactggaa tcccaaagta
     2461 ggctctgacg ccggtgaagg gatggacttg ccagcaaggt aaggacaagc aagccaagag
     2521 caaacgctcc ctacttccat gtccttgtat aggctgccag cagaaggcat ggcgcagact
     2581 agaggtacgt tttcccactt gaagatcaga attaaaggaa gacctgccaa cttcaagtta
     2641 agcaaaaatc cctcccaggt gtgccctcca ttgttagatt ttagttcaat ccagatggag
     2701 tcaagttgac aaccaagaat ggccaccacc ggggacactc cgcatctgaa ctatatacta
     2761 agcttcatat ttcagacgtg tttcttaatg ttgttggtgt ctccagccct cgtagtgttt
     2821 gtatcgtatt ctctgcagtt tagcatgcca gtgtttcctg tccttcacgt aaatgccttt
     2881 gtgtgaagct tgcttgacac cccgccccca ccccacctct caactcccct atccctgtga
     2941 cactgcccag taataactgt tcgttgttgt ttacctgttg cttgtaagtg caagtattaa
     3001 agcaatttta aagctaaatt cacctgtaag aatataataa atatctgtaa tcagaaaaaa
     3061 aa
//
