LOCUS       NM_026270               1610 bp    mRNA    linear   ROD 31-MAY-2024
DEFINITION  Mus musculus AKT1 substrate 1 (Akt1s1), transcript variant 2, mRNA.
ACCESSION   NM_026270
VERSION     NM_026270.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Yang,L., Liao,J., Huang,H., Lee,T.L. and Qi,H.
  TITLE     Stage-specific regulation of undifferentiated spermatogonia by
            AKT1S1-mediated AKT-mTORC1 signaling during mouse spermatogenesis
  JOURNAL   Dev Biol 509, 11-27 (2024)
   PUBMED   38311163
  REMARK    GeneRIF: Stage-specific regulation of undifferentiated
            spermatogonia by AKT1S1-mediated AKT-mTORC1 signaling during mouse
            spermatogenesis.
REFERENCE   2  (bases 1 to 1610)
  AUTHORS   Cai,S., Yang,Q., Cao,Y., Li,Y., Liu,J., Wang,J., Zhang,X., Liu,L.,
            Li,X. and Zhang,Y.
  TITLE     PF4 antagonizes retinal neovascularization via inhibiting PRAS40
            phosphorylation in a mouse model of oxygen-induced retinopathy
  JOURNAL   Biochim Biophys Acta Mol Basis Dis 1866 (3), 165604 (2020)
   PUBMED   31740404
  REMARK    GeneRIF: This study, for the first time, links PF4's anti-RNV
            function to an intracellular signaling molecule PRAS40 and its
            phosphorylation.
REFERENCE   3  (bases 1 to 1610)
  AUTHORS   Figlia,G., Norrmen,C., Pereira,J.A., Gerber,D. and Suter,U.
  TITLE     Dual function of the PI3K-Akt-mTORC1 axis in myelination of the
            peripheral nervous system
  JOURNAL   Elife 6, e29241 (2017)
   PUBMED   28880149
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1610)
  AUTHORS   Yang,W., Yang,L.F., Song,Z.Q., Shah,S.Z.A., Cui,Y.Y., Li,C.S.,
            Zhao,H.F., Gao,H.L., Zhou,X.M. and Zhao,D.M.
  TITLE     PRAS40 alleviates neurotoxic prion peptide-induced apoptosis via
            mTOR-AKT signaling
  JOURNAL   CNS Neurosci Ther 23 (5), 416-427 (2017)
   PUBMED   28294542
  REMARK    GeneRIF: PRAS40 inhibits mTORC1 hyperactivation and plays a key
            role in protecting cells against neurotoxic prion peptide-induced
            apoptosis. Thus, PRAS40 is a potential therapeutic target for prion
            disease.
REFERENCE   5  (bases 1 to 1610)
  AUTHORS   Cordon-Barris,L., Pascual-Guiral,S., Yang,S., Gimenez-Llort,L.,
            Lope-Piedrafita,S., Niemeyer,C., Claro,E., Lizcano,J.M. and
            Bayascas,J.R.
  TITLE     Mutation of the 3-Phosphoinositide-Dependent Protein Kinase 1
            (PDK1) Substrate-Docking Site in the Developing Brain Causes
            Microcephaly with Abnormal Brain Morphogenesis Independently of
            Akt, Leading to Impaired Cognition and Disruptive Behaviors
  JOURNAL   Mol Cell Biol 36 (23), 2967-2982 (2016)
   PUBMED   27644329
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1610)
  AUTHORS   Saito,A., Hayashi,T., Okuno,S., Nishi,T. and Chan,P.H.
  TITLE     Modulation of proline-rich akt substrate survival signaling
            pathways by oxidative stress in mouse brains after transient focal
            cerebral ischemia
  JOURNAL   Stroke 37 (2), 513-517 (2006)
   PUBMED   16397181
  REMARK    GeneRIF: overexpression of SOD1 may affect the PRAS pathway after
            transient focal cerebral ischemia by reducing the direct oxidative
            reaction to pPRAS after reperfusion injury
REFERENCE   7  (bases 1 to 1610)
  AUTHORS   Ballif,B.A., Villen,J., Beausoleil,S.A., Schwartz,D. and Gygi,S.P.
  TITLE     Phosphoproteomic analysis of the developing mouse brain
  JOURNAL   Mol Cell Proteomics 3 (11), 1093-1101 (2004)
   PUBMED   15345747
REFERENCE   8  (bases 1 to 1610)
  AUTHORS   Saito,A., Narasimhan,P., Hayashi,T., Okuno,S., Ferrand-Drake,M. and
            Chan,P.H.
  TITLE     Neuroprotective role of a proline-rich Akt substrate in apoptotic
            neuronal cell death after stroke: relationships with nerve growth
            factor
  JOURNAL   J Neurosci 24 (7), 1584-1593 (2004)
   PUBMED   14973226
  REMARK    GeneRIF: PRAS phosphorylation and its interaction with pAkt and
            14-3-3 might play an important role in neuroprotection mediated by
            NGF in apoptotic neuronal cell death after transient focal cerebral
            ischemia
REFERENCE   9  (bases 1 to 1610)
  AUTHORS   Kovacina,K.S., Park,G.Y., Bae,S.S., Guzzetta,A.W., Schaefer,E.,
            Birnbaum,M.J. and Roth,R.A.
  TITLE     Identification of a proline-rich Akt substrate as a 14-3-3 binding
            partner
  JOURNAL   J Biol Chem 278 (12), 10189-10194 (2003)
   PUBMED   12524439
  REMARK    GeneRIF: The protein product encoded by 1110012J22 gene is
            proline-rich substrate of Akt which also binds to 14-3-3.  A
            proposed name for this protein is thus PRAS40, for proline-rich
            substrate of Akt of 40 kDa.
REFERENCE   10 (bases 1 to 1610)
  AUTHORS   Chern,J.J. and Choi,K.W.
  TITLE     Lobe mediates Notch signaling to control domain-specific growth in
            the Drosophila eye disc
  JOURNAL   Development 129 (17), 4005-4013 (2002)
   PUBMED   12163404
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY103589.1, AK141771.1 and BE847693.1.
            
            [WARNING] On Feb 6, 2025 this sequence was replaced by NM_026270.5.
            
            On Dec 10, 2011 this sequence version replaced NM_026270.3.
            
            Transcript Variant: This variant (2) contains an alternate first
            exon compared to variant 3. The resulting isoform (b) is shorter at
            the N-terminus compared to isoform a. Variants 1 and 2 both encode
            the same isoform (b).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK141771.1, AK003638.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-5                 BY103589.1         1-5
            6-1584              AK141771.1         2-1580
            1585-1610           BE847693.1         4-29                c
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7"
     gene            1..1610
                     /gene="Akt1s1"
                     /gene_synonym="1110012J22Rik; Lobe; Lobel; PRAS40"
                     /note="AKT1 substrate 1"
                     /db_xref="GeneID:67605"
                     /db_xref="MGI:MGI:1914855"
     exon            1..125
                     /gene="Akt1s1"
                     /gene_synonym="1110012J22Rik; Lobe; Lobel; PRAS40"
                     /inference="alignment:Splign:2.1.0"
     exon            126..514
                     /gene="Akt1s1"
                     /gene_synonym="1110012J22Rik; Lobe; Lobel; PRAS40"
                     /inference="alignment:Splign:2.1.0"
     CDS             133..906
                     /gene="Akt1s1"
                     /gene_synonym="1110012J22Rik; Lobe; Lobel; PRAS40"
                     /note="isoform b is encoded by transcript variant 2;
                     proline-rich AKT1 substrate 1; proline-rich AKT substrate;
                     AKT1 substrate 1 (proline-rich)"
                     /codon_start=1
                     /product="proline-rich AKT1 substrate 1 isoform b"
                     /protein_id="NP_080546.1"
                     /db_xref="CCDS:CCDS21218.1"
                     /db_xref="GeneID:67605"
                     /db_xref="MGI:MGI:1914855"
                     /translation="MASGRPEELWEAVVGAAERFQARTGTELVLLTAAPPPPPRPGPC
                     AYAAHGRGALAEAARRCLHDIAQAHRAATATRPPGPPPAPQPPSPAPSPPPRPALARE
                     DEEEDEDEPTETETSGERLGGSDNGGLFMMDEDATLQDLPPFCESDPESTDDGSLSEE
                     TPAGPTACPQPPATALPTQQYAKSLPVSVPVWAFKEKRTEARSSDEENGPPSSPDLDR
                     IAASMRALVLREAEDTQVFGDLPRPRLNTSDFQKLKRKY"
     exon            515..592
                     /gene="Akt1s1"
                     /gene_synonym="1110012J22Rik; Lobe; Lobel; PRAS40"
                     /inference="alignment:Splign:2.1.0"
     exon            593..762
                     /gene="Akt1s1"
                     /gene_synonym="1110012J22Rik; Lobe; Lobel; PRAS40"
                     /inference="alignment:Splign:2.1.0"
     exon            763..1592
                     /gene="Akt1s1"
                     /gene_synonym="1110012J22Rik; Lobe; Lobel; PRAS40"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1564..1569
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Akt1s1"
                     /gene_synonym="1110012J22Rik; Lobe; Lobel; PRAS40"
                     /note="hexamer: ATTAAA"
     polyA_site      1589
                     /gene="Akt1s1"
                     /gene_synonym="1110012J22Rik; Lobe; Lobel; PRAS40"
                     /note="major polyA site"
ORIGIN      
        1 tgtgccgttt ttctcgagga caaaccagag cggcttggtg gaagccatgt taatgcgggg
       61 cactaccatc tctttgcggg gcacgacggc ggggtgctgg aaaagcgaaa cggagagagg
      121 aaaagggctc ggatggcgtc tgggcggcca gaggaactgt gggaagccgt cgtgggggcc
      181 gccgagcgct ttcaggcccg cactggcaca gagctggtat tactgactgc agcgccaccg
      241 ccgccgcccc gccctggacc ctgtgcctat gccgcccatg gccgcggagc cctggcagag
      301 gcggcccgac gctgcctcca cgacatcgca caggcgcaca gggctgccac tgccacccga
      361 cctcctggtc ccccaccagc accacagccg cccagccctg ctcctagtcc accacctcgg
      421 ccagccctgg ccagggagga tgaggaggaa gatgaggacg agcccactga aacagagaca
      481 tctggggagc ggctgggcgg tagcgataat ggaggtctct tcatgatgga tgaggatgcc
      541 accctccagg acctgccccc cttctgcgag tcagacccgg agagcacaga cgacggcagc
      601 ctgagcgagg agacgcccgc cggtcccaca gcctgtcccc agcccccggc cacagccctg
      661 cctacccagc agtatgccaa gtctctgccc gtgtcggtgc cagtgtgggc cttcaaggag
      721 aagaggacag aagcccgatc gtcagatgag gagaatggcc cgccctcctc gcccgaccta
      781 gaccgaatag cggccagcat gcgcgcgctg gtgctgcggg aggctgagga cacccaggtc
      841 ttcggggatc ttccgcggcc gcggctcaat accagcgact tccagaagct gaagcggaaa
      901 tattaagcga acagaaagcc ttggcctgcg agaggccact gcctctcggc ccagcaagag
      961 aacaattctt tccgggattg gctggctcag gttgggcggg gccggttcta aacgctccaa
     1021 gggtcaactc tgtttcttgc ctagcaaacg aagctttgcc cgcacagcct tgactcgcgg
     1081 ccccagggcg gagcccagct gcgggagctg ccgctgctca gccagtgtcg ggaagagttg
     1141 cccgccagcc ttccagagtt cccctacccg attggtcccg gcctcctggg agtgtggcca
     1201 agccctccct aacccagaat tggcccgagg cctttaattt cttacactac tagggctgtt
     1261 aatcaattgt ttttgttttt gttgttttct ttgcctgagt cctgacaatt gtatcccggt
     1321 tccccagggg acagtctggg tccctctctg gtctgacgct ccctataggt tccaccgcac
     1381 gacagcctgt ccatcaaaac ttgtcctgta cccaggacag taccagcttc ctgtcccccg
     1441 accctaacag gtgccttaaa aggccctctc ccacccaagg tgggggggcc aggggggcct
     1501 cactttccgg ccctagactt gggtgcggga gagtgggatg ggggtaggga ggggcgctct
     1561 gagattaaag ttttacctct gactagtgag ttaaaaaaaa aaaaaaaaaa
//
