LOCUS       NM_001382480            2264 bp    mRNA    linear   ROD 01-JUN-2024
DEFINITION  Mus musculus AKT1 substrate 1 (Akt1s1), transcript variant 5, mRNA.
ACCESSION   NM_001382480 XM_030242938
VERSION     NM_001382480.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Yang,L., Liao,J., Huang,H., Lee,T.L. and Qi,H.
  TITLE     Stage-specific regulation of undifferentiated spermatogonia by
            AKT1S1-mediated AKT-mTORC1 signaling during mouse spermatogenesis
  JOURNAL   Dev Biol 509, 11-27 (2024)
   PUBMED   38311163
  REMARK    GeneRIF: Stage-specific regulation of undifferentiated
            spermatogonia by AKT1S1-mediated AKT-mTORC1 signaling during mouse
            spermatogenesis.
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Cai,S., Yang,Q., Cao,Y., Li,Y., Liu,J., Wang,J., Zhang,X., Liu,L.,
            Li,X. and Zhang,Y.
  TITLE     PF4 antagonizes retinal neovascularization via inhibiting PRAS40
            phosphorylation in a mouse model of oxygen-induced retinopathy
  JOURNAL   Biochim Biophys Acta Mol Basis Dis 1866 (3), 165604 (2020)
   PUBMED   31740404
  REMARK    GeneRIF: This study, for the first time, links PF4's anti-RNV
            function to an intracellular signaling molecule PRAS40 and its
            phosphorylation.
REFERENCE   3  (bases 1 to 2264)
  AUTHORS   Figlia,G., Norrmen,C., Pereira,J.A., Gerber,D. and Suter,U.
  TITLE     Dual function of the PI3K-Akt-mTORC1 axis in myelination of the
            peripheral nervous system
  JOURNAL   Elife 6, e29241 (2017)
   PUBMED   28880149
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2264)
  AUTHORS   Yang,W., Yang,L.F., Song,Z.Q., Shah,S.Z.A., Cui,Y.Y., Li,C.S.,
            Zhao,H.F., Gao,H.L., Zhou,X.M. and Zhao,D.M.
  TITLE     PRAS40 alleviates neurotoxic prion peptide-induced apoptosis via
            mTOR-AKT signaling
  JOURNAL   CNS Neurosci Ther 23 (5), 416-427 (2017)
   PUBMED   28294542
  REMARK    GeneRIF: PRAS40 inhibits mTORC1 hyperactivation and plays a key
            role in protecting cells against neurotoxic prion peptide-induced
            apoptosis. Thus, PRAS40 is a potential therapeutic target for prion
            disease.
REFERENCE   5  (bases 1 to 2264)
  AUTHORS   Cordon-Barris,L., Pascual-Guiral,S., Yang,S., Gimenez-Llort,L.,
            Lope-Piedrafita,S., Niemeyer,C., Claro,E., Lizcano,J.M. and
            Bayascas,J.R.
  TITLE     Mutation of the 3-Phosphoinositide-Dependent Protein Kinase 1
            (PDK1) Substrate-Docking Site in the Developing Brain Causes
            Microcephaly with Abnormal Brain Morphogenesis Independently of
            Akt, Leading to Impaired Cognition and Disruptive Behaviors
  JOURNAL   Mol Cell Biol 36 (23), 2967-2982 (2016)
   PUBMED   27644329
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2264)
  AUTHORS   Saito,A., Hayashi,T., Okuno,S., Nishi,T. and Chan,P.H.
  TITLE     Modulation of proline-rich akt substrate survival signaling
            pathways by oxidative stress in mouse brains after transient focal
            cerebral ischemia
  JOURNAL   Stroke 37 (2), 513-517 (2006)
   PUBMED   16397181
  REMARK    GeneRIF: overexpression of SOD1 may affect the PRAS pathway after
            transient focal cerebral ischemia by reducing the direct oxidative
            reaction to pPRAS after reperfusion injury
REFERENCE   7  (bases 1 to 2264)
  AUTHORS   Ballif,B.A., Villen,J., Beausoleil,S.A., Schwartz,D. and Gygi,S.P.
  TITLE     Phosphoproteomic analysis of the developing mouse brain
  JOURNAL   Mol Cell Proteomics 3 (11), 1093-1101 (2004)
   PUBMED   15345747
REFERENCE   8  (bases 1 to 2264)
  AUTHORS   Saito,A., Narasimhan,P., Hayashi,T., Okuno,S., Ferrand-Drake,M. and
            Chan,P.H.
  TITLE     Neuroprotective role of a proline-rich Akt substrate in apoptotic
            neuronal cell death after stroke: relationships with nerve growth
            factor
  JOURNAL   J Neurosci 24 (7), 1584-1593 (2004)
   PUBMED   14973226
  REMARK    GeneRIF: PRAS phosphorylation and its interaction with pAkt and
            14-3-3 might play an important role in neuroprotection mediated by
            NGF in apoptotic neuronal cell death after transient focal cerebral
            ischemia
REFERENCE   9  (bases 1 to 2264)
  AUTHORS   Kovacina,K.S., Park,G.Y., Bae,S.S., Guzzetta,A.W., Schaefer,E.,
            Birnbaum,M.J. and Roth,R.A.
  TITLE     Identification of a proline-rich Akt substrate as a 14-3-3 binding
            partner
  JOURNAL   J Biol Chem 278 (12), 10189-10194 (2003)
   PUBMED   12524439
  REMARK    GeneRIF: The protein product encoded by 1110012J22 gene is
            proline-rich substrate of Akt which also binds to 14-3-3.  A
            proposed name for this protein is thus PRAS40, for proline-rich
            substrate of Akt of 40 kDa.
REFERENCE   10 (bases 1 to 2264)
  AUTHORS   Chern,J.J. and Choi,K.W.
  TITLE     Lobe mediates Notch signaling to control domain-specific growth in
            the Drosophila eye disc
  JOURNAL   Development 129 (17), 4005-4013 (2002)
   PUBMED   12163404
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC158231.2.
            
            On May 12, 2020 this sequence version replaced XM_030242938.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR13422589.273285.1,
                                           SRR7345562.890979.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849377
                                           [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-800               AC158231.2         123071-123870
            801-1189            AC158231.2         125270-125658
            1190-1267           AC158231.2         126479-126556
            1268-1437           AC158231.2         126638-126807
            1438-2264           AC158231.2         127291-128117
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7"
     gene            1..2264
                     /gene="Akt1s1"
                     /gene_synonym="1110012J22Rik; Lobe; Lobel; PRAS40"
                     /note="AKT1 substrate 1"
                     /db_xref="GeneID:67605"
                     /db_xref="MGI:MGI:1914855"
     exon            1..800
                     /gene="Akt1s1"
                     /gene_synonym="1110012J22Rik; Lobe; Lobel; PRAS40"
                     /inference="alignment:Splign:2.1.0"
     exon            801..1189
                     /gene="Akt1s1"
                     /gene_synonym="1110012J22Rik; Lobe; Lobel; PRAS40"
                     /inference="alignment:Splign:2.1.0"
     CDS             808..1581
                     /gene="Akt1s1"
                     /gene_synonym="1110012J22Rik; Lobe; Lobel; PRAS40"
                     /note="isoform b is encoded by transcript variant 5;
                     proline-rich AKT1 substrate 1; proline-rich AKT substrate;
                     AKT1 substrate 1 (proline-rich)"
                     /codon_start=1
                     /product="proline-rich AKT1 substrate 1 isoform b"
                     /protein_id="NP_001369409.1"
                     /db_xref="GeneID:67605"
                     /db_xref="MGI:MGI:1914855"
                     /translation="MASGRPEELWEAVVGAAERFQARTGTELVLLTAAPPPPPRPGPC
                     AYAAHGRGALAEAARRCLHDIAQAHRAATATRPPGPPPAPQPPSPAPSPPPRPALARE
                     DEEEDEDEPTETETSGERLGGSDNGGLFMMDEDATLQDLPPFCESDPESTDDGSLSEE
                     TPAGPTACPQPPATALPTQQYAKSLPVSVPVWAFKEKRTEARSSDEENGPPSSPDLDR
                     IAASMRALVLREAEDTQVFGDLPRPRLNTSDFQKLKRKY"
     exon            1190..1267
                     /gene="Akt1s1"
                     /gene_synonym="1110012J22Rik; Lobe; Lobel; PRAS40"
                     /inference="alignment:Splign:2.1.0"
     exon            1268..1437
                     /gene="Akt1s1"
                     /gene_synonym="1110012J22Rik; Lobe; Lobel; PRAS40"
                     /inference="alignment:Splign:2.1.0"
     exon            1438..2264
                     /gene="Akt1s1"
                     /gene_synonym="1110012J22Rik; Lobe; Lobel; PRAS40"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2239..2244
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Akt1s1"
                     /gene_synonym="1110012J22Rik; Lobe; Lobel; PRAS40"
                     /note="hexamer: ATTAAA"
     polyA_site      2264
                     /gene="Akt1s1"
                     /gene_synonym="1110012J22Rik; Lobe; Lobel; PRAS40"
                     /note="major polyA site"
ORIGIN      
        1 agagcagggg cctctctggg ctgcgggcgt ggtgtagccc tggtcggcgg cctcacttcg
       61 gtggtaggat ccttcttgga cgtcccgcac cgtgtcccgt gctgcggccg ttttcttttc
      121 tatctctctt cacaactgtc ctgggaggcc tgcaccttcc cgctggacag ctcccccttc
      181 ctggtttgcc ccagagtctt gcctcatagg aggccatagg cctaggaggt tggagtaagt
      241 tgccctcatt ccagccgcct tcacaagtca ttcactcggt gacactgctg ctgtggctgc
      301 ccccgagatc tcgtgcaaag aggctgagac ttgccactct ttgccctcac gaaagtgagg
      361 accgaagctg tgtcatcttg gatcagtcac tttcttgccc ttagccataa tcctaaactt
      421 ccagcctcct ttattagcta ggagaggtgg aaggtgtgtg taggagttgc tgggcgcctt
      481 ttaaagctgt aggtaggact ttgaggccca gcagacagta gtgtcaaagc actgcagctt
      541 tctccctctc catggaaaga tgggatgggg ggatgcagtc taatgaggta ggcagggtgg
      601 agcgcagtat tggcagagaa ggctctagac acagactgct cttctcagtg gtcagctctg
      661 ccagtataac agtcaagagc ctggattcag agcttcaggc tgtaaagtgg ggtgtccatt
      721 gctgattcat ggaggaagtg aggggttgct taggaacggt gatcatcttc tggtccaaca
      781 tatgagtcgt tcccactttg ggctcggatg gcgtctgggc ggccagagga actgtgggaa
      841 gccgtcgtgg gggccgccga gcgctttcag gcccgcactg gcacagagct ggtattactg
      901 actgcagcgc caccgccgcc gccccgccct ggaccctgtg cctatgccgc ccatggccgc
      961 ggagccctgg cagaggcggc ccgacgctgc ctccacgaca tcgcacaggc gcacagggct
     1021 gccactgcca cccgacctcc tggtccccca ccagcaccac agccgcccag ccctgctcct
     1081 agtccaccac ctcggccagc cctggccagg gaggatgagg aggaagatga ggacgagccc
     1141 actgaaacag agacatctgg ggagcggctg ggcggtagcg ataatggagg tctcttcatg
     1201 atggatgagg atgccaccct ccaggacctg ccccccttct gcgagtcaga cccggagagc
     1261 acagacgacg gcagcctgag cgaggagacg cccgccggtc ccacagcctg tccccagccc
     1321 ccggccacag ccctgcctac ccagcagtat gccaagtctc tgcccgtgtc ggtgccagtg
     1381 tgggccttca aggagaagag gacagaagcc cgatcgtcag atgaggagaa tggcccgccc
     1441 tcctcgcccg acctagaccg aatagcggcc agcatgcgcg cgctggtgct gcgggaggct
     1501 gaggacaccc aggtcttcgg ggatcttccg cggccgcggc tcaataccag cgacttccag
     1561 aagctgaagc ggaaatatta agcgaacaga aagccttggc ctgcgagagg ccactgcctc
     1621 tcggcccagc aagagaacaa ttctttccgg gattggctgg ctcaggttgg gcggggccgg
     1681 ttctaaacgc tccaagggtc aactctgttt cttgcctagc aaacgaagct ttgcccgcac
     1741 agccttgact cgcggcccca gggcggagcc cagctgcggg agctgccgct gctcagccag
     1801 tgtcgggaag agttgcccgc cagccttcca gagttcccct acccgattgg tcccggcctc
     1861 ctgggagtgt ggccaagccc tccctaaccc agaattggcc cgaggccttt aatttcttac
     1921 actactaggg ctgttaatca attgtttttg tttttgttgt tttctttgcc tgagtcctga
     1981 caattgtatc ccggttcccc aggggacagt ctgggtccct ctctggtctg acgctcccta
     2041 taggttccac cgcacgacag cctgtccatc aaaacttgtc ctgtacccag gacagtacca
     2101 gcttcctgtc ccccgaccct aacaggtgcc ttaaaaggcc ctctcccacc caaggtgggg
     2161 gggccagggg ggcctcactt tccggcccta gacttgggtg cgggagagtg ggatgggggt
     2221 agggaggggc gctctgagat taaagtttta cctctgacta gtga
//
