LOCUS       NM_001042542            2859 bp    mRNA    linear   ROD 21-AUG-2024
DEFINITION  Mus musculus A kinase anchor protein 4 (Akap4), transcript variant
            2, mRNA.
ACCESSION   NM_001042542 XM_972744
VERSION     NM_001042542.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2859)
  AUTHORS   Wei,H., Zhang,X., Wang,C., Wang,J., Li,T., Chen,S., Li,H. and
            Wang,B.
  TITLE     A pathogenic AKAP4 variant, p.R429H, causes male in/subfertility in
            humans and mice
  JOURNAL   Clin Transl Med 13 (12), e1463 (2023)
   PUBMED   38050179
REFERENCE   2  (bases 1 to 2859)
  AUTHORS   Ma,N., Liu,H., Zhang,Y., Liu,W., Liang,Z., Wang,Q., Sun,Y.,
            Wang,L., Li,Y., Ren,H. and Dong,Y.
  TITLE     Identification of CD8+ T-cell epitope from multiple
            myeloma-specific antigen AKAP4
  JOURNAL   Front Immunol 13, 927804 (2022)
   PUBMED   35967402
  REMARK    GeneRIF: Identification of CD8(+) T-cell epitope from multiple
            myeloma-specific antigen AKAP4.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2859)
  AUTHORS   Zhang,G., Li,D., Tu,C., Meng,L., Tan,Y., Ji,Z., Cheng,J., Lu,G.,
            Lin,G., Zhang,H., Sun,J., Wang,M., Du,J. and Xu,W.
  TITLE     Loss-of-function missense variant of AKAP4 induced male infertility
            through reduced interaction with QRICH2 during sperm flagella
            development
  JOURNAL   Hum Mol Genet 31 (2), 219-231 (2021)
   PUBMED   34415320
  REMARK    GeneRIF: Loss-of-function missense variant of AKAP4 induced male
            infertility through reduced interaction with QRICH2 during sperm
            flagella development.
REFERENCE   4  (bases 1 to 2859)
  AUTHORS   Scovell,J.M., Bournat,J.C., Szafran,A.T., Solis,M., Moore,J.,
            Rivera,A., Chen,C.H., Zhang,J., Wilken,N., Seth,A. and Jorgez,C.J.
  TITLE     PRSS50 is a testis protease responsible for proper sperm tail
            formation and function
  JOURNAL   Development 148 (8) (2021)
   PUBMED   33913480
REFERENCE   5  (bases 1 to 2859)
  AUTHORS   Choowong-In,P., Sattayasai,J., Poodendaen,C. and Iamsaard,S.
  TITLE     Decreased expression of AKAP4 and TyrPho proteins in testis,
            epididymis, and spermatozoa with low sexual performance of mice
            induced by modified CUMS
  JOURNAL   Andrologia 53 (3), e13977 (2021)
   PUBMED   33486757
  REMARK    GeneRIF: Decreased expression of AKAP4 and TyrPho proteins in
            testis, epididymis, and spermatozoa with low sexual performance of
            mice induced by modified CUMS.
REFERENCE   6  (bases 1 to 2859)
  AUTHORS   Turner,R.M., Johnson,L.R., Haig-Ladewig,L., Gerton,G.L. and
            Moss,S.B.
  TITLE     An X-linked gene encodes a major human sperm fibrous sheath
            protein, hAKAP82. Genomic organization, protein kinase A-RII
            binding, and distribution of the precursor in the sperm tail
  JOURNAL   J Biol Chem 273 (48), 32135-32141 (1998)
   PUBMED   9822690
REFERENCE   7  (bases 1 to 2859)
  AUTHORS   Sutton,K.A. and Wilkinson,M.F.
  TITLE     The rapidly evolving Pem homeobox gene and Agtr2, Ant2, and Lamp2
            are closely linked in the proximal region of the mouse X chromosome
  JOURNAL   Genomics 45 (2), 447-450 (1997)
   PUBMED   9344676
REFERENCE   8  (bases 1 to 2859)
  AUTHORS   Moss,S.B., VanScoy,H. and Gerton,G.L.
  TITLE     Mapping of a haploid transcribed and translated sperm-specific gene
            to the mouse X chromosome
  JOURNAL   Mamm Genome 8 (1), 37-38 (1997)
   PUBMED   9021145
REFERENCE   9  (bases 1 to 2859)
  AUTHORS   Fulcher,K.D., Mori,C., Welch,J.E., O'Brien,D.A., Klapper,D.G. and
            Eddy,E.M.
  TITLE     Characterization of Fsc1 cDNA for a mouse sperm fibrous sheath
            component
  JOURNAL   Biol Reprod 52 (1), 41-49 (1995)
   PUBMED   7711182
REFERENCE   10 (bases 1 to 2859)
  AUTHORS   Carrera,A., Gerton,G.L. and Moss,S.B.
  TITLE     The major fibrous sheath polypeptide of mouse sperm: structural and
            functional similarities to the A-kinase anchoring proteins
  JOURNAL   Dev Biol 165 (1), 272-284 (1994)
   PUBMED   8088444
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL808124.12.
            
            On Dec 18, 2013 this sequence version replaced NM_001042542.1.
            
            Transcript Variant: This variant (2) differs in the 5' UTR, lacks a
            portion of the 5' coding region and initiates translation at a
            downstream start codon compared to variant 1. The encoded isoform
            (b) has a shorter N-terminus compared to isoform a.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR5189685.165568.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849381, SAMN00849384
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-146               AL808124.12        15814-15959
            147-242             AL808124.12        18035-18130
            243-293             AL808124.12        20753-20803
            294-395             AL808124.12        21239-21340
            396-2513            AL808124.12        23313-25430
            2514-2859           AL808124.12        25868-26213
FEATURES             Location/Qualifiers
     source          1..2859
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="X"
                     /map="X 3.17 cM"
     gene            1..2859
                     /gene="Akap4"
                     /gene_synonym="AKAP-4; AKAP82; Fsc1; mAKAP82; p82; PRKA4"
                     /note="A kinase anchor protein 4"
                     /db_xref="GeneID:11643"
                     /db_xref="MGI:MGI:102794"
     exon            1..146
                     /gene="Akap4"
                     /gene_synonym="AKAP-4; AKAP82; Fsc1; mAKAP82; p82; PRKA4"
                     /inference="alignment:Splign:2.1.0"
     CDS             147..2669
                     /gene="Akap4"
                     /gene_synonym="AKAP-4; AKAP82; Fsc1; mAKAP82; p82; PRKA4"
                     /note="isoform b is encoded by transcript variant 2;
                     A-kinase anchor protein 82 kDa; major sperm fibrous sheath
                     protein; protein kinase A-anchoring protein 4; A kinase
                     (PRKA) anchor protein 4; A kinase anchoring protein 4"
                     /codon_start=1
                     /product="A-kinase anchor protein 4 isoform b"
                     /protein_id="NP_001036007.1"
                     /db_xref="CCDS:CCDS40839.1"
                     /db_xref="GeneID:11643"
                     /db_xref="MGI:MGI:102794"
                     /translation="MSDDIDWLHSRRGVCKVDLYSPKGQQDQDRKVICFVDVSTLNVE
                     DKDSKGAAGSRSEGELNLETLEEKEIIVIKDTEKQDQSKTEGSVCLFKQAPSDPISVL
                     NWLLNDLQKYALGFQHALSPSASSCKHKVGDLEGDYSKIPSENCYSVYADQVNFDYLN
                     KGPQNLRLEMAASKNTNNNQSPSNPATKSPSNQRSVATPEGECSMDDLSFYVNRLSSL
                     VIQMARKEIKDKLEGGSKCLHHSMYTSGDKGKTSPRSAVSKIASEMAHEAVELTSSEM
                     RGNGEDCRDGRKTFLYSEMCNKNKCGEKQQMCPKDSKEFADSISKGLMVYANQVASDM
                     MVSVMKTLKVHSCGKPIPACVVLKRVLLKHTKEIVSDLIDSCMKNLHNITGVLMTDSD
                     FVSAVKRNLFNHGKQNAADIMEAMLKRLVSALLGEKKETKSQSLAYATLKAGTNDPKC
                     KNQSLEFSAMKAEMKGKDKCKSKADPCCKSLTSAERVSEHILKESLTMWNNQKQGNQG
                     KVTNKVCCTSKDEKREKISPSTDSLAKDLIVSALMLIQYHLTQQAKGKDPCEEECPGS
                     SMGYMSQSAQYEKCGGGQSSKSLSMKHFETRGAPGPSTCMKENQLESQKMDMSNMVLS
                     LIQKLLSESPFSCDELTESDNKRCCDPRSSKAAPMAKRPEEQCQDNAELDFISGMKQM
                     NRQFIDQLVESVMKLCLIMAKYSNNGAALAELEEQAALVGSGSRCGRDAMMSQNYSET
                     PGPEVIVNNQCSTTNLQKQLQAVLQWIAASQFNVPMLYFMGDDDGQLEKLPEVSAKAA
                     EKGYSVGDLLQEVMKFAKERQLDEAVGNMARKQLLDWLLANL"
     exon            147..242
                     /gene="Akap4"
                     /gene_synonym="AKAP-4; AKAP82; Fsc1; mAKAP82; p82; PRKA4"
                     /inference="alignment:Splign:2.1.0"
     exon            243..293
                     /gene="Akap4"
                     /gene_synonym="AKAP-4; AKAP82; Fsc1; mAKAP82; p82; PRKA4"
                     /inference="alignment:Splign:2.1.0"
     exon            294..395
                     /gene="Akap4"
                     /gene_synonym="AKAP-4; AKAP82; Fsc1; mAKAP82; p82; PRKA4"
                     /inference="alignment:Splign:2.1.0"
     exon            396..2513
                     /gene="Akap4"
                     /gene_synonym="AKAP-4; AKAP82; Fsc1; mAKAP82; p82; PRKA4"
                     /inference="alignment:Splign:2.1.0"
     exon            2514..2859
                     /gene="Akap4"
                     /gene_synonym="AKAP-4; AKAP82; Fsc1; mAKAP82; p82; PRKA4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2842..2847
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Akap4"
                     /gene_synonym="AKAP-4; AKAP82; Fsc1; mAKAP82; p82; PRKA4"
                     /note="hexamer: AATAAA"
     polyA_site      2853
                     /gene="Akap4"
                     /gene_synonym="AKAP-4; AKAP82; Fsc1; mAKAP82; p82; PRKA4"
     polyA_site      2859
                     /gene="Akap4"
                     /gene_synonym="AKAP-4; AKAP82; Fsc1; mAKAP82; p82; PRKA4"
                     /note="major polyA site"
ORIGIN      
        1 atcagtctgg tctaacagct gaccggggtg gcagccagct gcaagtgcct aagaacttgg
       61 cactgccccc ttccatctaa aggggcacat ctcacttctg ggtgacacac actcagtcaa
      121 aggtacaaaa caactctatc atcaagatgt ctgacgatat tgactggtta cacagccgca
      181 gaggcgtgtg caaggtagat ctttacagtc caaaaggaca acaagatcag gaccgaaaag
      241 tgatatgctt tgtggatgtg tctaccttga atgtggaaga taaagattcc aagggtgctg
      301 ctggttccag gtcagaaggc gagttaaatc tggagactct ggaagaaaaa gagattatcg
      361 taatcaagga cactgaaaaa caagaccagt ctaagacgga gggatctgtg tgccttttca
      421 aacaagctcc ctctgatcct ataagtgtcc tcaactggct cctcaatgat ctccagaagt
      481 atgccttggg tttccaacat gcattaagcc cctcagcctc tagctgtaaa cataaagtgg
      541 gagacctaga gggtgactat agcaaaatac catctgagaa ctgctacagt gtgtacgctg
      601 atcaagtaaa ctttgattat ttgaacaaag gacctcaaaa ccttcgccta gaaatggcgg
      661 catccaaaaa caccaacaat aaccaaagtc cctcaaatcc tgcaaccaaa tctcctagca
      721 atcagaggtc agttgccact cctgagggag aatgttctat ggacgacctt tccttctatg
      781 tcaaccgatt gtcatctctg gtaatccaaa tggcccgtaa ggaaatcaag gacaaattgg
      841 aaggtggaag caaatgtctc catcattcca tgtatacatc aggagataaa gggaaaacca
      901 gcccccggag tgctgtcagc aaaattgctt cggagatggc ccatgaagct gttgaactga
      961 cctcatcaga aatgcgtggc aatggagagg attgcaggga tggccggaaa acctttttgt
     1021 atagtgaaat gtgtaacaag aacaagtgtg gcgaaaagca gcagatgtgc ccaaaagaca
     1081 gcaaagaatt tgcggattcc atcagcaagg ggcttatggt ttatgcaaat caagtagcat
     1141 ctgacatgat ggtctctgtt atgaaaactt tgaaagtaca cagctgtggg aagccaattc
     1201 cggcttgtgt ggtcctgaag agggtactat tgaagcacac caaggaaatt gtatctgatc
     1261 tgattgattc atgtatgaaa aacttgcata acataacagg agtcctgatg acagactcag
     1321 acttcgtttc tgctgtcaag aggaatcttt tcaatcatgg taaacaaaat gcagcggaca
     1381 tcatggaggc catgctaaag cgtctggtca gtgctcttct tggtgagaag aaggagacta
     1441 agtctcaaag tctggcctat gcaaccttaa aagctggaac taatgatccg aaatgcaaga
     1501 atcagagcct tgagttctca gctatgaaag ctgaaatgaa aggaaaagat aaatgcaaat
     1561 ccaaagcaga tccatgctgc aaatcgttga caagtgctga gagagtcagc gagcatatcc
     1621 tcaaagagag ccttactatg tggaacaacc agaagcaagg aaaccaaggc aaagtgacta
     1681 acaaagtttg ctgcaccagt aaagatgaga agagagaaaa gatcagtcct tccacagatt
     1741 cactggccaa ggatctaatt gtctctgccc ttatgctcat tcaataccat ctgacccaac
     1801 aagccaaggg caaagatcca tgtgaagagg agtgccctgg ttcctccatg gggtatatgt
     1861 cccaaagtgc acaatacgaa aaatgtggag gtggccaaag ttctaaatca ctttcaatga
     1921 agcattttga aactcgtgga gctcctggac catctacatg tatgaaggaa aatcaactgg
     1981 agtcccagaa gatggatatg tcaaacatgg ttctgtccct gattcagaaa ctcctgagtg
     2041 agagcccttt cagttgcgat gaactaactg aaagtgacaa taagcgttgt tgtgatccta
     2101 gatcaagcaa agcagctccc atggccaaga gacctgaaga gcaatgccaa gacaatgcag
     2161 aactagactt catcagtggg atgaagcaaa tgaaccgcca gtttatagat cagctggtag
     2221 aatccgtgat gaaactctgc ctgatcatgg ctaagtacag caacaatgga gcagccctgg
     2281 ctgagctgga agaacaagca gccctggtag gcagtggctc cagatgtggc cgtgatgcta
     2341 tgatgtcaca aaattattct gaaactcctg gccctgaagt tattgtcaac aatcagtgct
     2401 ctacaactaa cttgcagaag cagctgcagg ctgtcctgca atggattgca gcctctcaat
     2461 tcaatgtgcc catgctctac ttcatgggag acgatgatgg acaactagag aagctccctg
     2521 aagtttcagc taaggctgca gagaaggggt acagtgtagg agatcttctt caggaggtca
     2581 tgaagtttgc caaagaacga caactggatg aagccgtggg caacatggct agaaagcagc
     2641 tgctagactg gcttctcgct aacctgtaag ctgagaattc ctttgactcc cctccatcca
     2701 tcctcccccc cagcagcaat tccaccccag ctggagccac cctcaccatc aggctggtga
     2761 actgcacaat tgggatcaca tttaccaata catctgagca gttgcactgt gaaaatactg
     2821 ggtgccctcc tgggcaacat gaataaaaaa attcaaaaa
//
