LOCUS       NM_019921               3879 bp    mRNA    linear   ROD 07-FEB-2025
DEFINITION  Mus musculus A kinase anchor protein 10 (Akap10), mRNA; nuclear
            gene for mitochondrial product.
ACCESSION   NM_019921
VERSION     NM_019921.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3879)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3879)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643.. PMID: 29144450]
REFERENCE   3  (bases 1 to 3879)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   4  (bases 1 to 3879)
  AUTHORS   Patel,H.H., Hamuro,L.L., Chun,B.J., Kawaraguchi,Y., Quick,A.,
            Rebolledo,B., Pennypacker,J., Thurston,J., Rodriguez-Pinto,N.,
            Self,C., Olson,G., Insel,P.A., Giles,W.R., Taylor,S.S. and
            Roth,D.M.
  TITLE     Disruption of protein kinase A localization using a trans-activator
            of transcription (TAT)-conjugated A-kinase-anchoring peptide
            reduces cardiac function
  JOURNAL   J Biol Chem 285 (36), 27632-27640 (2010)
   PUBMED   20581396
REFERENCE   5  (bases 1 to 3879)
  AUTHORS   Tingley,W.G., Pawlikowska,L., Zaroff,J.G., Kim,T., Nguyen,T.,
            Young,S.G., Vranizan,K., Kwok,P.Y., Whooley,M.A. and Conklin,B.R.
  TITLE     Gene-trapped mouse embryonic stem cell-derived cardiac myocytes and
            human genetics implicate AKAP10 in heart rhythm regulation
  JOURNAL   Proc Natl Acad Sci U S A 104 (20), 8461-8466 (2007)
   PUBMED   17485678
  REMARK    GeneRIF: These studies suggest a role for AKAP10 in heart rhythm
            control.
REFERENCE   6  (bases 1 to 3879)
  AUTHORS   Gisler,S.M., Madjdpour,C., Bacic,D., Pribanic,S., Taylor,S.S.,
            Biber,J. and Murer,H.
  TITLE     PDZK1: II. an anchoring site for the PKA-binding protein D-AKAP2 in
            renal proximal tubular cells
  JOURNAL   Kidney Int 64 (5), 1746-1754 (2003)
   PUBMED   14531807
  REMARK    GeneRIF: D-AKAP2 may play an important role in the parathyroid
            hormone (PTH)-mediated regulation of NaPi-IIa by
            compartmentalization of PKA.
REFERENCE   7  (bases 1 to 3879)
  AUTHORS   Burns,L.L., Canaves,J.M., Pennypacker,J.K., Blumenthal,D.K. and
            Taylor,S.S.
  TITLE     Isoform specific differences in binding of a dual-specificity
            A-kinase anchoring protein to type I and type II regulatory
            subunits of PKA
  JOURNAL   Biochemistry 42 (19), 5754-5763 (2003)
   PUBMED   12741833
REFERENCE   8  (bases 1 to 3879)
  AUTHORS   Hamuro,Y., Burns,L., Canaves,J., Hoffman,R., Taylor,S. and Woods,V.
  TITLE     Domain organization of D-AKAP2 revealed by enhanced deuterium
            exchange-mass spectrometry (DXMS)
  JOURNAL   J Mol Biol 321 (4), 703-714 (2002)
   PUBMED   12206784
  REMARK    GeneRIF: deuterium exchange-mass spectrometry (DXMS) and limited
            proteolysis to probe the folded regions of D-AKAP2, providing for
            the first time insight into the intra-domain dynamics of a scaffold
            protein
REFERENCE   9  (bases 1 to 3879)
  AUTHORS   Wang,L., Sunahara,R.K., Krumins,A., Perkins,G., Crochiere,M.L.,
            Mackey,M., Bell,S., Ellisman,M.H. and Taylor,S.S.
  TITLE     Cloning and mitochondrial localization of full-length D-AKAP2, a
            protein kinase A anchoring protein
  JOURNAL   Proc Natl Acad Sci U S A 98 (6), 3220-3225 (2001)
   PUBMED   11248059
REFERENCE   10 (bases 1 to 3879)
  AUTHORS   Huang,L.J., Durick,K., Weiner,J.A., Chun,J. and Taylor,S.S.
  TITLE     D-AKAP2, a novel protein kinase A anchoring protein with a putative
            RGS domain
  JOURNAL   Proc Natl Acad Sci U S A 94 (21), 11184-11189 (1997)
   PUBMED   9326583
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL646042.14 and AK049399.1.
            
            On May 23, 2013 this sequence version replaced NM_019921.2.
            
            Summary: This gene encodes a member of A-kinase anchoring proteins
            (AKAPs), a family of functionally related proteins that target
            protein kinase A to discrete locations within the cell. The encoded
            protein is localized to mitochondria and interacts with both the
            type I and type II regulatory subunits of PKA. It has been reported
            that this protein is important for maintaining heart rate and
            myocardial contractility through its targeting of protein kinase A.
            In mouse, defects of this gene lead to cardiac arrhythmias and
            premature death. In humans, polymorphisms in this gene may be
            associated with increased risk of arrhythmias and sudden cardiac
            death. [provided by RefSeq, May 2013].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK049399.1, SRR13861890.71119.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: PMID: 11248059; reported by
                                                  MitoCarta
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-31                AL646042.14        130282-130312       c
            32-3879             AK049399.1         1-3848
FEATURES             Location/Qualifiers
     source          1..3879
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 37.96 cM"
     gene            1..3879
                     /gene="Akap10"
                     /gene_synonym="1500031L16Rik; B130049N18Rik; D-AKAP-2;
                     D-AKAP2; PRKA10"
                     /note="A kinase anchor protein 10"
                     /db_xref="GeneID:56697"
                     /db_xref="MGI:MGI:1890218"
     exon            1..269
                     /gene="Akap10"
                     /gene_synonym="1500031L16Rik; B130049N18Rik; D-AKAP-2;
                     D-AKAP2; PRKA10"
                     /inference="alignment:Splign:2.1.0"
     CDS             182..2170
                     /gene="Akap10"
                     /gene_synonym="1500031L16Rik; B130049N18Rik; D-AKAP-2;
                     D-AKAP2; PRKA10"
                     /note="A-kinase anchor protein 10, mitochondrial; protein
                     kinase A-anchoring protein 10; dual specificity A
                     kinase-anchoring protein 2; A kinase (PRKA) anchor protein
                     10"
                     /codon_start=1
                     /product="A-kinase anchor protein 10, mitochondrial
                     precursor"
                     /protein_id="NP_064305.2"
                     /db_xref="CCDS:CCDS24821.1"
                     /db_xref="GeneID:56697"
                     /db_xref="MGI:MGI:1890218"
                     /translation="MRGAGPSPRHSPRALRPDPGPAMSFFRRKVKGKEQEKTLDVKST
                     KASVAVHSPQKSTKNHALLEAAGPSHVAINAISANMDSFSSSRTATLKKQPSHMEAAH
                     FGDLGRSCLDYQTQETKSSLSKTLEQVLRDTVVLPYFLQFMELRRMEHLVKFWLEAES
                     FHSTTWSRIRAHSLNTVKQSSLAEPVSPSKRHETPASSVTEALDRRLGDSSSAPLLVT
                     QSEGTDLSSRTQNPQNHLLLSQEGHSARSLHREVARTGSHQIPTDSQDSSSRLAVGSR
                     NSCSSPLRELSEKLMKSIEQDAVNTFTKYISPDAAKPIPITEAMRNDIIAKICGEDGQ
                     VDPNCFVLAQAVVFSAMEQEHFSEFLRSHHFCKYQIEVLTSGTVYLADILFCESALFY
                     FSEYMEKEDAVNILQFWLAADNFQSQLAAKKGQYDGQEAQNDAMILYDKYFSLQATHP
                     LGFDDVVRLEIESNICREGGPLPNCFTTPLRQAWTTMEKVFLPGFLSSNLYYKYLNDL
                     IHSVRGDEFLGGNVSLAAHGSVCLPEESHSGGSDGSTAQSSVKKASIKILKNFDEAII
                     VDAASLDPESLYQRTYAGKMSFGRVSDLGQFIRESEPEPDVKKSKGFMFSQAMKKWVQ
                     GNTDEAQEELAWKIAKMIVSDVMQQAHHDQPLEKSTKL"
     exon            270..317
                     /gene="Akap10"
                     /gene_synonym="1500031L16Rik; B130049N18Rik; D-AKAP-2;
                     D-AKAP2; PRKA10"
                     /inference="alignment:Splign:2.1.0"
     exon            318..500
                     /gene="Akap10"
                     /gene_synonym="1500031L16Rik; B130049N18Rik; D-AKAP-2;
                     D-AKAP2; PRKA10"
                     /inference="alignment:Splign:2.1.0"
     exon            501..1058
                     /gene="Akap10"
                     /gene_synonym="1500031L16Rik; B130049N18Rik; D-AKAP-2;
                     D-AKAP2; PRKA10"
                     /inference="alignment:Splign:2.1.0"
     exon            1059..1157
                     /gene="Akap10"
                     /gene_synonym="1500031L16Rik; B130049N18Rik; D-AKAP-2;
                     D-AKAP2; PRKA10"
                     /inference="alignment:Splign:2.1.0"
     exon            1158..1242
                     /gene="Akap10"
                     /gene_synonym="1500031L16Rik; B130049N18Rik; D-AKAP-2;
                     D-AKAP2; PRKA10"
                     /inference="alignment:Splign:2.1.0"
     exon            1243..1366
                     /gene="Akap10"
                     /gene_synonym="1500031L16Rik; B130049N18Rik; D-AKAP-2;
                     D-AKAP2; PRKA10"
                     /inference="alignment:Splign:2.1.0"
     exon            1367..1503
                     /gene="Akap10"
                     /gene_synonym="1500031L16Rik; B130049N18Rik; D-AKAP-2;
                     D-AKAP2; PRKA10"
                     /inference="alignment:Splign:2.1.0"
     exon            1504..1648
                     /gene="Akap10"
                     /gene_synonym="1500031L16Rik; B130049N18Rik; D-AKAP-2;
                     D-AKAP2; PRKA10"
                     /inference="alignment:Splign:2.1.0"
     exon            1649..1822
                     /gene="Akap10"
                     /gene_synonym="1500031L16Rik; B130049N18Rik; D-AKAP-2;
                     D-AKAP2; PRKA10"
                     /inference="alignment:Splign:2.1.0"
     exon            1823..1932
                     /gene="Akap10"
                     /gene_synonym="1500031L16Rik; B130049N18Rik; D-AKAP-2;
                     D-AKAP2; PRKA10"
                     /inference="alignment:Splign:2.1.0"
     exon            1933..2015
                     /gene="Akap10"
                     /gene_synonym="1500031L16Rik; B130049N18Rik; D-AKAP-2;
                     D-AKAP2; PRKA10"
                     /inference="alignment:Splign:2.1.0"
     exon            2016..2068
                     /gene="Akap10"
                     /gene_synonym="1500031L16Rik; B130049N18Rik; D-AKAP-2;
                     D-AKAP2; PRKA10"
                     /inference="alignment:Splign:2.1.0"
     exon            2069..2164
                     /gene="Akap10"
                     /gene_synonym="1500031L16Rik; B130049N18Rik; D-AKAP-2;
                     D-AKAP2; PRKA10"
                     /inference="alignment:Splign:2.1.0"
     exon            2165..3879
                     /gene="Akap10"
                     /gene_synonym="1500031L16Rik; B130049N18Rik; D-AKAP-2;
                     D-AKAP2; PRKA10"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      2260
                     /gene="Akap10"
                     /gene_synonym="1500031L16Rik; B130049N18Rik; D-AKAP-2;
                     D-AKAP2; PRKA10"
                     /note="major polyA site"
     regulatory      3485..3490
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Akap10"
                     /gene_synonym="1500031L16Rik; B130049N18Rik; D-AKAP-2;
                     D-AKAP2; PRKA10"
                     /note="hexamer: AATATA"
     polyA_site      3507
                     /gene="Akap10"
                     /gene_synonym="1500031L16Rik; B130049N18Rik; D-AKAP-2;
                     D-AKAP2; PRKA10"
     regulatory      3760..3765
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Akap10"
                     /gene_synonym="1500031L16Rik; B130049N18Rik; D-AKAP-2;
                     D-AKAP2; PRKA10"
                     /note="hexamer: CATAAA"
     polyA_site      3784
                     /gene="Akap10"
                     /gene_synonym="1500031L16Rik; B130049N18Rik; D-AKAP-2;
                     D-AKAP2; PRKA10"
ORIGIN      
        1 ctcccgtgaa gccccgcgcg cggacggacc ggaactctaa gcgggtggct tgttgataac
       61 atggcggcag cagctgccgg ggcatcccga ggtggcggca gcgcccacac ccggaagaag
      121 gggctgtttt cggactagtg cagcggctcc tgcggccccg gaagtcccgg ccagctgctg
      181 aatgagggga gccgggccct ccccgcgaca ctccccccgc gccctccgcc ccgacccggg
      241 ccccgccatg tccttcttcc ggcggaaagt gaaaggtaaa gagcaagaga agaccttaga
      301 tgtcaagtcc actaaagctt cagtagccgt acattcccca caaaaaagca ctaaaaatca
      361 tgccttgctg gaggctgcag gaccaagtca tgttgcgatc aatgccattt ctgccaacat
      421 ggactccttt tcaagtagca ggacagcaac gcttaagaaa cagccaagcc acatggaggc
      481 tgctcatttt ggagacctag gcagatcctg tctggactat cagactcaag agaccaaatc
      541 cagtctttca aagacccttg aacaagtctt gcgtgacact gttgtccttc catatttcct
      601 tcagtttatg gaacttcgga gaatggagca cctggtgaaa ttttggttag aggctgaaag
      661 ttttcactct acaacgtggt cccgaataag agcacacagt ctaaacacag tgaaacagag
      721 ttcactggct gagcctgtct ctccatctaa aagacatgag actccagcat cttctgtaac
      781 tgaggctctt gacaggaggt tgggggattc tagctcagcc ccactgcttg tgactcaatc
      841 agaaggaact gacctgagta gtagaactca gaaccctcag aaccacttgc tgctttccca
      901 ggaaggccac agtgcccgtt ctctccatcg tgaggtggcc agaacaggaa gtcatcagat
      961 tcccaccgac tcccaggact cctcctccag acttgcagta ggcagtagaa acagttgctc
     1021 ttctccactg agagaactgt cagaaaaact aatgaaaagt atagaacaag atgcagtgaa
     1081 tacttttacc aaatatatat ctccagatgc tgctaagcca ataccaatta cagaagccat
     1141 gagaaacgac atcatcgcaa agatttgtgg agaagatgga caggtggatc ccaactgttt
     1201 cgttctggca caggctgtag tctttagtgc aatggagcaa gagcacttta gtgagtttct
     1261 gcgaagtcac catttctgta aataccagat tgaagtgctg accagtggga ctgtttacct
     1321 ggctgatatc ctcttctgtg agtcagccct cttttatttt tctgagtaca tggaaaaaga
     1381 agatgcagtg aatatcttac aattctggtt agcagcggat aatttccagt ctcagcttgc
     1441 tgccaaaaag ggccagtatg atggacagga ggcccagaat gatgccatga ttttatatga
     1501 caagtacttt tccctccaag ccacacaccc ccttggattt gatgatgttg tacgattaga
     1561 aattgaatct aatatctgca gggaaggtgg accacttcct aattgtttca caactccatt
     1621 acgtcaggcc tggacaacca tggagaaggt ctttttgcct ggttttctgt ccagcaatct
     1681 ttattacaaa tatttgaatg atctcatcca ttcagttcga ggagatgaat ttcttggagg
     1741 gaatgtttcc ctggctgctc acggctctgt ctgccttcct gaggagtctc actcaggtgg
     1801 ttccgatggc tccactgctc agtctagtgt gaaaaaagcc agtattaaaa ttctgaaaaa
     1861 ttttgatgaa gcaataattg tggatgctgc aagtctggac ccagaatctt tatatcaacg
     1921 gacatatgca gggaagatgt cctttgggag agttagtgat ttggggcagt tcatccgaga
     1981 gtctgagcct gaacctgatg tgaagaaatc aaaaggattc atgttctcac aagctatgaa
     2041 gaagtgggtg caaggaaata ctgacgaggc ccaagaagag ctagcttgga agattgcaaa
     2101 aatgatagtg agtgatgtta tgcagcaggc acaccatgat caaccactag agaagtctac
     2161 aaagctatga ctcgaaacca gagataagga aatctgattt tgaaaaaaat aagaactttc
     2221 ccctttggtt ggattcttca acacagccga tgaaaacgga gcactgcatt tcttttcact
     2281 gctagatcac tgttgtttcc agggaagagt gggagacagt ccgagagtgg ttgttaaatg
     2341 tagacataga tgacacacat gacagtaaca cagtgagtcc cgagatacaa agtggtggtg
     2401 gcgacttatt agaccatttt ccagtggcca cgctcactgg ggtagacagg gaccacatgc
     2461 tcgcagcaga ttacttcctc agctgtgtca gaaagcatga cagaggtgct tcattcttgt
     2521 ctcctagagg tcacaggtga gtcagccctg cctggagcga gcatctccaa gtggcagtgt
     2581 gggccagcag cctgtggagc agaggacaat tccttgccta gtcttccttc acagaagcat
     2641 taatttagaa agctggcaag tcgtgggtca agcctcagtg ttgcatcctt tccttcgctg
     2701 acagaacaca ctgttcacct ttccttcact cccgaggtga tcgatcctta aagctagtcc
     2761 ttcctctgct gacaagaggg cgagtttagt ttactttgag catcaagacc cattccgcta
     2821 tcagaacgct atgctccaga cgcttcattc cctcttcaga atacacatgg aaaaaatgag
     2881 cactccagtt tttccctgtg gtccttagaa cacattcaca aaattggttt tattttttaa
     2941 taaggtgggg acagaagcac tcccttgtgg gtgtctcccc aattcttcac tgtgagtggg
     3001 aaatgtgcag ttgccttttc cctccctgtc ttaagacagt aggagactca ctcgctgccc
     3061 ttctgcttag ttggctttgc acttgtggca cagccgttgg cggggccacc ccgctggcaa
     3121 gcacactccc ccagcctcac ggtgccagtg cttccttgtg tgtaacagct tagagagaaa
     3181 ggaagttttc ttaggtgtta ttgcttttgc tcagaatttg caaaagggtg tgtgtgtgtg
     3241 tgtgtgtgtg tgtgtgtgtg tatacacaca tgtacatata taattattaa tcacctctgt
     3301 ccttggggaa agtcttgaat gaacagaaaa tttatttcat tgcaatattg attgtacaga
     3361 ggcacactgt tcaacagaag aagcaaaaag gctgtgtata agtttttggt actatgtacc
     3421 atctctgtta ttctgaagta ttcatgtact taaaccatat tctatttaat tgtgtttgat
     3481 tttaaatata tatatatatg aattatattt aaaatggtgt caacttcctg ctttcagggc
     3541 atttctggct ctcctgttga agtatattga cccttcctaa tatttccact tgttttacaa
     3601 aactccagaa cataatcccc aattggacat agccttgtgt ttgggtgtag acatagacat
     3661 gaggttttaa ttagtgtatg tgctgttgtg gctcttctgt tcctttaaca aagcatttct
     3721 ctccatttca tatcgcttca aactttaagt gaaaagcaac ataaagacat agagtcaaac
     3781 gtgattctga gtgtcctctg cctttgcatg cttcagggta aaaaatctgt aatgctctcg
     3841 gctgtttaaa ctagtgggtt tgttaaaata ctttctagt
//
