LOCUS       NM_001370813            6003 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Mus musculus adenylate kinase 9 (Ak9), mRNA.
ACCESSION   NM_001370813
VERSION     NM_001370813.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 6003)
  AUTHORS   Yang,H.W., Lee,S., Berry,B.C., Yang,D., Zheng,S., Carroll,R.S.,
            Park,P.J. and Johnson,M.D.
  TITLE     A role for mutations in AK9 and other genes affecting ependymal
            cells in idiopathic normal pressure hydrocephalus
  JOURNAL   Proc Natl Acad Sci U S A 120 (51), e2300681120 (2023)
   PUBMED   38100419
  REMARK    GeneRIF: A role for mutations in AK9 and other genes affecting
            ependymal cells in idiopathic normal pressure hydrocephalus.
REFERENCE   2  (bases 1 to 6003)
  AUTHORS   O'Callaghan,E., Navarrete-Lopez,P., Stiavnicka,M., Sanchez,J.M.,
            Maroto,M., Pericuesta,E., Fernandez-Gonzalez,R., O'Meara,C.,
            Eivers,B., Kelleher,M.M., Evans,R.D., Mapel,X.M., Lloret-Villas,A.,
            Pausch,H., Balastegui-Alarcon,M., Aviles,M., Sanchez-Rodriguez,A.,
            Roldan,E.R.S., McDonald,M., Kenny,D.A., Fair,S., Gutierrez-Adan,A.
            and Lonergan,P.
  TITLE     Adenylate kinase 9 is essential for sperm function and male
            fertility in mammals
  JOURNAL   Proc Natl Acad Sci U S A 120 (42), e2305712120 (2023)
   PUBMED   37812723
  REMARK    GeneRIF: Adenylate kinase 9 is essential for sperm function and
            male fertility in mammals.
REFERENCE   3  (bases 1 to 6003)
  AUTHORS   Sha,Y., Liu,W., Li,S., Osadchuk,L.V., Chen,Y., Nie,H., Gao,S.,
            Xie,L., Qin,W., Zhou,H. and Li,L.
  TITLE     Deficiency in AK9 causes asthenozoospermia and male infertility by
            destabilising sperm nucleotide homeostasis
  JOURNAL   EBioMedicine 96, 104798 (2023)
   PUBMED   37713809
  REMARK    Erratum:[EBioMedicine. 2024 Jan;99:104957. doi:
            10.1016/j.ebiom.2023.104957.. PMID: 38171079]
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC122332.4 and AC153360.6.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            RNAseq introns :: mixed sample support SAMN00849374, SAMN00849375
                              [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-72                AC122332.4         86537-86608
            73-203              AC122332.4         98839-98969
            204-267             AC122332.4         100385-100448
            268-320             AC122332.4         102873-102925
            321-417             AC122332.4         103024-103120
            418-530             AC122332.4         107653-107765
            531-716             AC122332.4         110165-110350
            717-845             AC122332.4         118505-118633
            846-920             AC122332.4         120166-120240
            921-1019            AC122332.4         123484-123582
            1020-1159           AC122332.4         127715-127854
            1160-1340           AC122332.4         127936-128116
            1341-1485           AC122332.4         129723-129867
            1486-1587           AC122332.4         130598-130699
            1588-1671           AC122332.4         130789-130872
            1672-1820           AC122332.4         134305-134453
            1821-1977           AC122332.4         140105-140261
            1978-2092           AC122332.4         141139-141253
            2093-2169           AC122332.4         149721-149797
            2170-2331           AC122332.4         152647-152808
            2332-2418           AC122332.4         165405-165491
            2419-2619           AC122332.4         165957-166157
            2620-2741           AC122332.4         166512-166633
            2742-2954           AC122332.4         171555-171767
            2955-3215           AC122332.4         175085-175345
            3216-3340           AC122332.4         178764-178888
            3341-3560           AC122332.4         182275-182494
            3561-3626           AC122332.4         184835-184900
            3627-3833           AC122332.4         189431-189637
            3834-4052           AC122332.4         189953-190171
            4053-4266           AC122332.4         191548-191761
            4267-4468           AC122332.4         203091-203292
            4469-4615           AC122332.4         204976-205122
            4616-4836           AC122332.4         205485-205705
            4837-5033           AC122332.4         207197-207393
            5034-5203           AC122332.4         209831-210000
            5204-5302           AC122332.4         210914-211012
            5303-5405           AC122332.4         213356-213458
            5406-5520           AC153360.6         156924-157038
            5521-6003           AC153360.6         157569-158051
FEATURES             Location/Qualifiers
     source          1..6003
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="10"
                     /map="10 22.23 cM"
     gene            1..6003
                     /gene="Ak9"
                     /gene_synonym="633979; Akd1; Akd2; Gm234; Gm7127"
                     /note="adenylate kinase 9"
                     /db_xref="GeneID:633979"
                     /db_xref="MGI:MGI:2685080"
     exon            1..72
                     /gene="Ak9"
                     /gene_synonym="633979; Akd1; Akd2; Gm234; Gm7127"
                     /inference="alignment:Splign:2.1.0"
     exon            73..203
                     /gene="Ak9"
                     /gene_synonym="633979; Akd1; Akd2; Gm234; Gm7127"
                     /inference="alignment:Splign:2.1.0"
     CDS             84..5723
                     /gene="Ak9"
                     /gene_synonym="633979; Akd1; Akd2; Gm234; Gm7127"
                     /EC_number="2.7.4.6"
                     /EC_number="2.7.4.4"
                     /note="adenylate kinase domain containing 2; adenylate
                     kinase domain containing 1"
                     /codon_start=1
                     /product="adenylate kinase 9"
                     /protein_id="NP_001357742.1"
                     /db_xref="GeneID:633979"
                     /db_xref="MGI:MGI:2685080"
                     /translation="MASEEKTEELHPFTDIFNEDETDRNCLLSKPTCFIIFGKPGAGK
                     TTLARNIAQAWKCIRVEALSVLEEHIAAEKETGAMLQSLLVSGHSIPDELVTKLILEK
                     IKSPEVAHFGYILTEMPSLAQDNMTSLKQIELVKNLELQPDIIINIKCSDYDLCQRTC
                     GQRQHSTTGYVYTREQWDPEIIESRRRKKRDFPKEGKSEEEEEEEEQEEEEAFIAEMQ
                     MVAEILQHLVQRPEDYLENVEITVKLYKELLLSALEEVMAEHNPQYLIELDGNKSPEE
                     LFMTVIERLKYLNVRRAAVITKLQGTEEEMTDIIDTEELFRTVSSYKLIAPRYRWYRS
                     KWARSCPVSLKDGNIYSGAADYTVSFLGKMYCLSSEETLKLFSLNPRPFLLPPMPLPP
                     CKVFIFGPEHIGKTTLANLIAEHFKAKVIDYAKLVQPRFDSAREKLIKDTITEATNTA
                     IKVVQQRLLNEKQAKQQEERTLKELQVQYSKREYNDFLSKSSEELPSLENTGSKLSSL
                     EIGQEDKSKSETTITGDQVKDVSTEESIEEVTENHPEVFAILEDTLRHAKELNFEQPH
                     DKQAEILEEVIKEETEDNKNRFPGAPKHGGWIVENFPLVRELWLSLIEKALLPDLVVY
                     LSDAESNGKHVLNRLYLKNKAEIDGKILERLQEEAQAKKREEEEIRKVKEEELRLEEE
                     KQRLMELATKKSKDPDYPDNEAEEEVEDSEIHEESEAQEIPEYTRGSSLPEASETSET
                     PEGDHEPEAEFKPPGDTAVEAETEKDPKEGLGSEDLLKVMLPEFPEDGYPNVPEMEPL
                     KEVLNNHILSWKQLEQTIMDNVVSTLTVDVSNKTPQELLQRVVETMERPFKYSAWELN
                     AEDYEEEAEDYQAETEIDEEQEEEEEEEEEGEEKIKEKRRHMGDTKHFCPVVLKENFV
                     LQPGNPDEAVKYREKIYYFSSTEAKEKFLEHPEDYVSQNEPLKAPPLRICLLGPHGSG
                     KTVCARKLAENFGIFHIQFDEFLQEKMLLKAERKFGPEFEDDSEEEQLVKQELEELAA
                     QANVKIEEDTTKKQLPDVQLTEEEEAIKLSLTDNEPLPSEILDSILSEWWLKEPIRST
                     GFILDGFPRHPEEAQFLGERGFFPDAAVIIQVDDQDIFDRLLPAQVQKWKTKQHKKLE
                     RKKLIKDLKTKIKEDMVAKRRAELILEREKKRREDGTFRDEDEFSDEDADYEDDIENI
                     LEEEFPKDEEEMSEEDEEQEADATERLRNELGEKFETETNNIQSIQDEFDKVLIPIIM
                     VNGARKMHIVQYVMNMKLKPLVENRESIFEKCYPVSSHLAHKMLSYTYKHMSSFGYWD
                     PVKLSEGETIKPVENAENPLNPVIHRHYIYFLSNKQTKEKFMMNPIKYIRQPKPKATM
                     PVRIMIVGPPKSGKTTVAKKLASDYGLRCLSVGDALRGMLNNHPDSELSLMLNWHLHK
                     GKTVPDELAIQALDISLMESVCNTIGVVIDGYPVTPHQMDLLEARSIIPMVIFELHVP
                     SKEIFKRLLLEKKTEQSMSYPLHNSSQIIAYKNAKYHKNINEIRQFYQKQHQNWHVID
                     GFHSKWWVWNEVLKDIQQMNKYIQTYMKKIKEGKAACIDKLCITPEELIARLGEFKQF
                     CPVSLAESNELIDCSVTSSLEFAAEFRGHYYKMSSQEKLNKFLKDPELYVPPLAPHPL
                     PPDDLLPKRLTLSELKSRFPKCAELQGYCPVTYQDGKQRYEALVPGNIRYALEYRNCI
                     YICESEEKLQKFMRSPMQYWNQKLPRKLPPLKESIHLTSLPLPGYLEQGTATSLIKAM
                     NAAGNLKPKLPFLSVRRSVLLYIAFHLKAFNPKGSEYSRKKYKKKLEQFVERCELITY
                     LSSKMTRKYKEPQFRAIDFDHKLQTFLSLKNIDPVTG"
     exon            204..267
                     /gene="Ak9"
                     /gene_synonym="633979; Akd1; Akd2; Gm234; Gm7127"
                     /inference="alignment:Splign:2.1.0"
     exon            268..320
                     /gene="Ak9"
                     /gene_synonym="633979; Akd1; Akd2; Gm234; Gm7127"
                     /inference="alignment:Splign:2.1.0"
     exon            321..417
                     /gene="Ak9"
                     /gene_synonym="633979; Akd1; Akd2; Gm234; Gm7127"
                     /inference="alignment:Splign:2.1.0"
     exon            418..530
                     /gene="Ak9"
                     /gene_synonym="633979; Akd1; Akd2; Gm234; Gm7127"
                     /inference="alignment:Splign:2.1.0"
     exon            531..716
                     /gene="Ak9"
                     /gene_synonym="633979; Akd1; Akd2; Gm234; Gm7127"
                     /inference="alignment:Splign:2.1.0"
     exon            717..845
                     /gene="Ak9"
                     /gene_synonym="633979; Akd1; Akd2; Gm234; Gm7127"
                     /inference="alignment:Splign:2.1.0"
     exon            846..920
                     /gene="Ak9"
                     /gene_synonym="633979; Akd1; Akd2; Gm234; Gm7127"
                     /inference="alignment:Splign:2.1.0"
     exon            921..1019
                     /gene="Ak9"
                     /gene_synonym="633979; Akd1; Akd2; Gm234; Gm7127"
                     /inference="alignment:Splign:2.1.0"
     exon            1020..1159
                     /gene="Ak9"
                     /gene_synonym="633979; Akd1; Akd2; Gm234; Gm7127"
                     /inference="alignment:Splign:2.1.0"
     exon            1160..1340
                     /gene="Ak9"
                     /gene_synonym="633979; Akd1; Akd2; Gm234; Gm7127"
                     /inference="alignment:Splign:2.1.0"
     exon            1341..1485
                     /gene="Ak9"
                     /gene_synonym="633979; Akd1; Akd2; Gm234; Gm7127"
                     /inference="alignment:Splign:2.1.0"
     exon            1486..1587
                     /gene="Ak9"
                     /gene_synonym="633979; Akd1; Akd2; Gm234; Gm7127"
                     /inference="alignment:Splign:2.1.0"
     exon            1588..1671
                     /gene="Ak9"
                     /gene_synonym="633979; Akd1; Akd2; Gm234; Gm7127"
                     /inference="alignment:Splign:2.1.0"
     exon            1672..1820
                     /gene="Ak9"
                     /gene_synonym="633979; Akd1; Akd2; Gm234; Gm7127"
                     /inference="alignment:Splign:2.1.0"
     exon            1821..1977
                     /gene="Ak9"
                     /gene_synonym="633979; Akd1; Akd2; Gm234; Gm7127"
                     /inference="alignment:Splign:2.1.0"
     exon            1978..2092
                     /gene="Ak9"
                     /gene_synonym="633979; Akd1; Akd2; Gm234; Gm7127"
                     /inference="alignment:Splign:2.1.0"
     exon            2093..2169
                     /gene="Ak9"
                     /gene_synonym="633979; Akd1; Akd2; Gm234; Gm7127"
                     /inference="alignment:Splign:2.1.0"
     exon            2170..2331
                     /gene="Ak9"
                     /gene_synonym="633979; Akd1; Akd2; Gm234; Gm7127"
                     /inference="alignment:Splign:2.1.0"
     exon            2332..2418
                     /gene="Ak9"
                     /gene_synonym="633979; Akd1; Akd2; Gm234; Gm7127"
                     /inference="alignment:Splign:2.1.0"
     exon            2419..2619
                     /gene="Ak9"
                     /gene_synonym="633979; Akd1; Akd2; Gm234; Gm7127"
                     /inference="alignment:Splign:2.1.0"
     exon            2620..2741
                     /gene="Ak9"
                     /gene_synonym="633979; Akd1; Akd2; Gm234; Gm7127"
                     /inference="alignment:Splign:2.1.0"
     exon            2742..2954
                     /gene="Ak9"
                     /gene_synonym="633979; Akd1; Akd2; Gm234; Gm7127"
                     /inference="alignment:Splign:2.1.0"
     exon            2955..3215
                     /gene="Ak9"
                     /gene_synonym="633979; Akd1; Akd2; Gm234; Gm7127"
                     /inference="alignment:Splign:2.1.0"
     exon            3216..3340
                     /gene="Ak9"
                     /gene_synonym="633979; Akd1; Akd2; Gm234; Gm7127"
                     /inference="alignment:Splign:2.1.0"
     exon            3341..3560
                     /gene="Ak9"
                     /gene_synonym="633979; Akd1; Akd2; Gm234; Gm7127"
                     /inference="alignment:Splign:2.1.0"
     exon            3561..3626
                     /gene="Ak9"
                     /gene_synonym="633979; Akd1; Akd2; Gm234; Gm7127"
                     /inference="alignment:Splign:2.1.0"
     exon            3627..3833
                     /gene="Ak9"
                     /gene_synonym="633979; Akd1; Akd2; Gm234; Gm7127"
                     /inference="alignment:Splign:2.1.0"
     exon            3834..4052
                     /gene="Ak9"
                     /gene_synonym="633979; Akd1; Akd2; Gm234; Gm7127"
                     /inference="alignment:Splign:2.1.0"
     exon            4053..4266
                     /gene="Ak9"
                     /gene_synonym="633979; Akd1; Akd2; Gm234; Gm7127"
                     /inference="alignment:Splign:2.1.0"
     exon            4267..4468
                     /gene="Ak9"
                     /gene_synonym="633979; Akd1; Akd2; Gm234; Gm7127"
                     /inference="alignment:Splign:2.1.0"
     exon            4469..4615
                     /gene="Ak9"
                     /gene_synonym="633979; Akd1; Akd2; Gm234; Gm7127"
                     /inference="alignment:Splign:2.1.0"
     exon            4616..4836
                     /gene="Ak9"
                     /gene_synonym="633979; Akd1; Akd2; Gm234; Gm7127"
                     /inference="alignment:Splign:2.1.0"
     exon            4837..5033
                     /gene="Ak9"
                     /gene_synonym="633979; Akd1; Akd2; Gm234; Gm7127"
                     /inference="alignment:Splign:2.1.0"
     exon            5034..5203
                     /gene="Ak9"
                     /gene_synonym="633979; Akd1; Akd2; Gm234; Gm7127"
                     /inference="alignment:Splign:2.1.0"
     exon            5204..5302
                     /gene="Ak9"
                     /gene_synonym="633979; Akd1; Akd2; Gm234; Gm7127"
                     /inference="alignment:Splign:2.1.0"
     exon            5303..5405
                     /gene="Ak9"
                     /gene_synonym="633979; Akd1; Akd2; Gm234; Gm7127"
                     /inference="alignment:Splign:2.1.0"
     exon            5406..5520
                     /gene="Ak9"
                     /gene_synonym="633979; Akd1; Akd2; Gm234; Gm7127"
                     /inference="alignment:Splign:2.1.0"
     exon            5521..6003
                     /gene="Ak9"
                     /gene_synonym="633979; Akd1; Akd2; Gm234; Gm7127"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5982..5987
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ak9"
                     /gene_synonym="633979; Akd1; Akd2; Gm234; Gm7127"
                     /note="hexamer: ATTAAA"
     polyA_site      6003
                     /gene="Ak9"
                     /gene_synonym="633979; Akd1; Akd2; Gm234; Gm7127"
                     /note="major polyA site"
ORIGIN      
        1 aaccgcctaa cagctaccta gagcctcagt agagcctcag aagatagctg ctgtcgcgtc
       61 cccggtccac agcattttaa acgatggctt ctgaagaaaa gacagaagaa cttcatccat
      121 tcacagacat ctttaatgaa gatgaaactg acaggaactg tttgctgtcc aaaccgactt
      181 gctttattat atttggaaaa cctggggctg gaaagacaac attagcccgt aacatagcac
      241 aggcatggaa atgtattcgt gttgaagctt tatcggtttt ggaagagcac attgccgctg
      301 agaaggagac cggagctatg ctgcagtcac tgttggtgag cggtcacagc atcccagacg
      361 agctggtcac aaagttgatc ctagagaaga tcaagtctcc agaagtagct cacttcggct
      421 acatcctcac tgaaatgcca tcacttgcac aagataacat gacctctttg aagcaaatag
      481 aacttgttaa aaatttagaa ttgcaacccg atatcataat caatattaag tgcagtgact
      541 acgatttatg ccaaagaact tgtgggcagc ggcaacacag cactacaggc tatgtataca
      601 ccagggagca gtgggaccca gaaatcattg agagtcgcag gagaaagaag agggacttcc
      661 ccaaggaagg gaagtctgaa gaggaagaag aggaagaaga acaagaagag gaggaggcat
      721 tcatagctga aatgcagatg gtggctgaga ttctccaaca cctcgtccag aggcccgagg
      781 attatttgga gaacgttgaa atcactgtga agctttataa ggagctactg ctcagtgctt
      841 tagaggaggt aatggctgag cacaatcccc agtatctcat cgagctagat gggaacaagt
      901 ctccagaaga gctcttcatg acagtcatag agcggctgaa gtacttgaac gtgagacgag
      961 cagctgtcat aaccaagctt cagggtacag aagaggaaat gactgacata atagatactg
     1021 aagagctctt ccgtacagtg tcgtcgtata aactcattgc gccaaggtat agatggtacc
     1081 gaagcaagtg ggcacgttct tgtccggtgt ccttaaagga tgggaacatt tattcaggag
     1141 cagcagacta tactgtgagt tttctaggta aaatgtactg cctttcttct gaagaaacgc
     1201 taaagttgtt ctcactgaac ccccgcccct tcctcctacc acctatgcct ttgccaccat
     1261 gcaaagtctt tatatttgga cctgagcaca tagggaaaac aacgctggcc aacctgattg
     1321 cggaacactt caaagcaaag gtaattgact acgccaaact tgttcagcca cgttttgaca
     1381 gtgcacgtga aaagctaata aaagacacca taactgaagc cactaacaca gccatcaaag
     1441 ttgtgcaaca gcggcttctg aatgagaagc aagccaaaca gcaagaggag agaaccctga
     1501 aggaactgca ggtgcaatac tcaaaaagag agtacaacga cttcttgtca aaaagttctg
     1561 aagaattacc ttctctggaa aacactggct ccaaattaag ctccttagag attggccagg
     1621 aagacaaatc aaagtcggag accaccatta ccggggacca ggttaaagat gtctccacag
     1681 aagagtcgat agaagaggtg actgaaaatc acccagaggt gtttgccata ttagaggata
     1741 ctctgagaca tgccaaggaa ttgaactttg agcagccaca tgataaacag gctgaaatac
     1801 tggaggaagt catcaaagag gagacagaag ataacaagaa taggttccct ggtgccccaa
     1861 aacatggagg atggattgtg gagaacttcc ctttagttcg agaactgtgg ttgtccttaa
     1921 tagaaaaagc acttctacct gatttggttg tttatttatc agatgcagaa agcaatggga
     1981 aacatgtgtt aaatagatta tatttaaaga ataaggcaga aatcgatggt aagattttag
     2041 aaagattgca agaagaagct caagccaaaa agagagagga ggaagaaatc cgaaaagtca
     2101 aagaagagga actgagactg gaagaagaaa aacaaaggct gatggaactt gcaactaaga
     2161 aatcaaaaga tcctgactat cctgataatg aagccgagga ggaggttgag gattctgaaa
     2221 ttcatgaaga gtctgaggca caagaaatac ctgagtacac ccgagggtca tcattgcctg
     2281 aggcgtctga aacatctgag accccagaag gagaccatga acctgaagca gaattcaagc
     2341 ctcctgggga cactgcagtg gaagcagaaa ctgaaaagga tcccaaagag ggcctggggt
     2401 ccgaagatct tctgaaagtc atgctgccag agtttccaga agatggctac cccaatgttc
     2461 ctgaaatgga gccactgaag gaggtgctga acaatcacat tctctcctgg aaacaactgg
     2521 agcagacgat tatggacaac gtagtttcga cattaacagt ggacgtttcc aacaaaacgc
     2581 cccaggagct cctgcagcgt gtagttgaga ccatggaaag gccttttaag tacagcgcct
     2641 gggaattaaa tgctgaagat tatgaagaag aagcagaaga ctatcaggct gagacagaaa
     2701 tcgatgagga gcaggaggaa gaggaagagg aagaagagga gggtgaagaa aaaattaaag
     2761 aaaagagaag acacatggga gataccaaac acttctgccc ggtggttctc aaagaaaact
     2821 ttgtcctaca accgggcaac ccggacgagg ctgttaaata ccgagaaaag atctactact
     2881 tctcgagcac tgaggcgaag gagaagtttt tagagcatcc tgaggactac gtgtcccaga
     2941 acgaaccgtt aaaggctcct ccgctaagga tttgtcttct gggtcctcac ggctctggca
     3001 aaactgtgtg tgcaagaaag ctggcagaaa actttggcat ttttcacatt cagtttgatg
     3061 aatttctcca agaaaaaatg ctgctcaagg ctgaaaggaa atttggacct gaatttgagg
     3121 atgattccga ggaggagcaa ttggtgaagc aagaacttga agagcttgca gctcaggcaa
     3181 atgtgaagat tgaagaggac actacaaaga agcagcttcc agatgtacaa ctcacagagg
     3241 aagaagaagc aatcaagttg agtctaactg acaatgagcc cctgccttca gaaattctag
     3301 actccattct ttctgagtgg tggcttaaag aaccaatacg ctccacaggc ttcatactgg
     3361 acggcttccc gcggcaccct gaggaggccc agtttctggg cgagcgtgga tttttcccag
     3421 atgccgccgt tataatacaa gtcgatgatc aagatatctt tgatcgtctc cttcctgccc
     3481 aagttcagaa gtggaagaca aaacagcaca agaaattaga gaggaaaaaa ctcatcaaag
     3541 acctgaagac aaaaatcaag gaggacatgg tcgctaaaag gagagctgaa cttattttgg
     3601 agagagagaa aaaaaggaga gaagacggca cctttagaga cgaagacgag tttagtgacg
     3661 aagatgctga ttatgaggat gatattgaga acatccttga ggaagagttt ccaaaggatg
     3721 aggaagagat gagcgaagaa gatgaggaac aggaagcaga cgctacggaa cgcctgagaa
     3781 atgagcttgg ggagaaattt gaaacagaaa caaataatat ccagtcgata caggatgaat
     3841 tcgacaaggt tttgatacca ataattatgg tcaacggagc taggaaaatg catattgtac
     3901 aatatgtaat gaatatgaaa ttgaagcccc tggtggaaaa ccgtgagagc atttttgaaa
     3961 agtgttaccc ggtatcctca cacctcgctc ataaaatgct ctcctacacc tacaaacaca
     4021 tgagttcatt tggctactgg gaccctgtaa agctaagcga aggagagaca atcaaacctg
     4081 ttgaaaatgc agagaacccg ctgaaccctg taatccatcg tcactacatc tatttcttat
     4141 ctaataagca aactaaagag aaatttatga tgaaccccat caaatacatc cggcaaccca
     4201 aacccaaagc cactatgcct gtgcggatta tgattgtggg gcctcccaag tcaggaaaaa
     4261 ctacagttgc caaaaagctt gcaagtgact acggcttaag atgtttgtcg gtgggagatg
     4321 cactgcgtgg aatgttaaac aatcacccag attcagagct gtcactgatg ttaaactggc
     4381 accttcacaa ggggaagacg gtgcctgatg aactggctat tcaagcttta gatatctccc
     4441 tgatggaaag tgtgtgcaac acaataggtg tggtcatcga tggctaccct gtcaccccac
     4501 accagatgga tctcttagag gcgaggtcaa tcattcccat ggtcatcttt gagttgcatg
     4561 tgccgtccaa ggagattttc aaaagactgc tcttagagaa gaagactgag caaagtatgt
     4621 cttatccact gcacaacagc tcacagatca tagcttataa gaatgcaaag taccataaaa
     4681 acatcaatga gatcaggcaa ttttatcaaa agcagcacca aaactggcat gtgattgacg
     4741 gctttcacag taaatggtgg gtatggaatg aagtcctcaa agacatccaa cagatgaata
     4801 agtacataca gacatacatg aaaaaaataa aggaagggaa agccgcatgc attgacaaat
     4861 tatgtatcac gcccgaggag ctgattgcgc gtctgggaga gtttaaacag ttctgccctg
     4921 tcagcctagc agaatcgaat gaactaattg attgctccgt aactagcagt ttggaatttg
     4981 cagcagagtt caggggacac tactataaaa tgagttccca ggaaaagcta aataaatttc
     5041 tgaaggaccc ggaactgtat gtgcctccct tggcacctca tccactccca ccagatgact
     5101 tgcttcccaa aaggcttaca ctgtcagagc tgaagagtcg ttttcctaag tgtgctgagc
     5161 tccagggcta ctgcccagtg acttaccagg atggaaaaca aagatatgaa gccctagtgc
     5221 ctggtaacat tcgatatgct ctagagtatc gtaattgcat atatatttgt gagagtgaag
     5281 aaaaactcca gaaattcatg aggtcaccaa tgcaatactg gaaccagaag cttccacgca
     5341 aacttccccc attaaaggaa tccatccacc tcaccagtct gcctttgcct gggtatctgg
     5401 aacaggggac agcaacttcc ctgatcaagg caatgaatgc ggcagggaac ctgaaaccca
     5461 agctcccctt cctaagtgtg aggaggtccg tgctccttta catagcgttt catctcaaag
     5521 cattcaatcc caagggttct gaatattcaa ggaaaaaata taagaagaag ctggagcagt
     5581 ttgtggaaag atgcgaactc ataacgtacc tcagttccaa gatgacccgg aagtacaagg
     5641 agcctcagtt cagagccatc gacttcgacc ataagctaca gacattttta tccttgaaaa
     5701 acatagaccc agttactggg tagcttgctt acgttacaga gacccaaatc ccagaactct
     5761 ccaagggaca gagggaattg aagagcagaa ttaataccct gcattttctg aagtatactt
     5821 ccctcctgcc taatgttgtg tatgtagttc ctgggcctca gatggactca aaaggcctaa
     5881 ccaggaaggg tcaccctctg agtattcatg cagctttcaa ctttattcct tttttccccc
     5941 taaatttgat gctcccacaa aataggcatt ccatttgaga tattaaaata cgtactacat
     6001 gta
//
