LOCUS       NM_001099299            3067 bp    mRNA    linear   ROD 12-AUG-2024
DEFINITION  Mus musculus adherens junction associated protein 1 (Ajap1), mRNA.
ACCESSION   NM_001099299 XM_001475023 XM_485506
VERSION     NM_001099299.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3067)
  AUTHORS   Fruh,S., Boudkkazi,S., Koppensteiner,P., Sereikaite,V., Chen,L.Y.,
            Fernandez-Fernandez,D., Rem,P.D., Ulrich,D., Schwenk,J., Chen,Z.,
            Le Monnier,E., Fritzius,T., Innocenti,S.M., Besseyrias,V.,
            Trovo,L., Stawarski,M., Argilli,E., Sherr,E.H., van Bon,B.,
            Kamsteeg,E.J., Iascone,M., Pilotta,A., Cutri,M.R., Azamian,M.S.,
            Hernandez-Garcia,A., Lalani,S.R., Rosenfeld,J.A., Zhao,X.,
            Vogel,T.P., Ona,H., Scott,D.A., Scheiffele,P., Stromgaard,K.,
            Tafti,M., Gassmann,M., Fakler,B., Shigemoto,R. and Bettler,B.
  TITLE     Monoallelic de novo AJAP1 loss-of-function variants disrupt
            trans-synaptic control of neurotransmitter release
  JOURNAL   Sci Adv 10 (28), eadk5462 (2024)
   PUBMED   38985877
  REMARK    GeneRIF: Monoallelic de novo AJAP1 loss-of-function variants
            disrupt trans-synaptic control of neurotransmitter release.
REFERENCE   2  (bases 1 to 3067)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 3067)
  AUTHORS   Zhang,M., Zhou,X., Jiang,W., Li,M., Zhou,R. and Zhou,S.
  TITLE     AJAP1 affects behavioral changes and GABABR1 level in epileptic
            mice
  JOURNAL   Biochem Biophys Res Commun 524 (4), 1057-1063 (2020)
   PUBMED   32067740
  REMARK    GeneRIF: AJAP1 affects behavioral changes and GABABR1 level in
            epileptic mice.
REFERENCE   4  (bases 1 to 3067)
  AUTHORS   Dinamarca,M.C., Raveh,A., Schneider,A., Fritzius,T., Fruh,S.,
            Rem,P.D., Stawarski,M., Lalanne,T., Turecek,R., Choo,M.,
            Besseyrias,V., Bildl,W., Bentrop,D., Staufenbiel,M., Gassmann,M.,
            Fakler,B., Schwenk,J. and Bettler,B.
  TITLE     Complex formation of APP with GABAB receptors links axonal
            trafficking to amyloidogenic processing
  JOURNAL   Nat Commun 10 (1), 1331 (2019)
   PUBMED   30902970
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3067)
  AUTHORS   Diman,N.Y., Brooks,G., Kruithof,B.P., Elemento,O., Seidman,J.G.,
            Seidman,C.E., Basson,C.T. and Hatcher,C.J.
  TITLE     Tbx5 is required for avian and Mammalian epicardial formation and
            coronary vasculogenesis
  JOURNAL   Circ Res 115 (10), 834-844 (2014)
   PUBMED   25245104
REFERENCE   6  (bases 1 to 3067)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   7  (bases 1 to 3067)
  AUTHORS   Schreiner,A., Ruonala,M., Jakob,V., Suthaus,J., Boles,E.,
            Wouters,F. and Starzinski-Powitz,A.
  TITLE     Junction protein shrew-1 influences cell invasion and interacts
            with invasion-promoting protein CD147
  JOURNAL   Mol Biol Cell 18 (4), 1272-1281 (2007)
   PUBMED   17267690
REFERENCE   8  (bases 1 to 3067)
  AUTHORS   Li,M.Z., Wang,J.S., Jiang,D.J., Xiang,C.X., Wang,F.Y., Zhang,K.H.,
            Williams,P.R. and Chen,Z.F.
  TITLE     Molecular mapping of developing dorsal horn-enriched genes by
            microarray and dorsal/ventral subtractive screening
  JOURNAL   Dev Biol 292 (2), 555-564 (2006)
   PUBMED   16516881
REFERENCE   9  (bases 1 to 3067)
  AUTHORS   Bharti,S., Handrow-Metzmacher,H., Zickenheiner,S., Zeitvogel,A.,
            Baumann,R. and Starzinski-Powitz,A.
  TITLE     Novel membrane protein shrew-1 targets to cadherin-mediated
            junctions in polarized epithelial cells
  JOURNAL   Mol Biol Cell 15 (1), 397-406 (2004)
   PUBMED   14595118
REFERENCE   10 (bases 1 to 3067)
  AUTHORS   Mu,X., Zhao,S., Pershad,R., Hsieh,T.F., Scarpa,A., Wang,S.W.,
            White,R.A., Beremand,P.D., Thomas,T.L., Gan,L. and Klein,W.H.
  TITLE     Gene expression in the developing mouse retina by EST sequencing
            and microarray analysis
  JOURNAL   Nucleic Acids Res 29 (24), 4983-4993 (2001)
   PUBMED   11812828
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL954390.19 and AL805910.7.
            
            On or before Jul 25, 2007 this sequence version replaced
            XM_001475023.1, XM_485506.4.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            CCDS Note: This CCDS ID represents the mouse ortholog of the
            protein described in PMIDs: 14595118 and 17267690. This variant is
            supported by BC147230.1 and BE648223.1. It should be noted that
            this transcript is predicted to undergo nonsense-mediated mRNA
            decay (NMD). However, the protein is represented because it was
            detected endogenously in human in PMID: 14595118. It is likely that
            the majority of transcripts representing this variant will undergo
            NMD, while some low level of NMD escape may allow for the
            expression of this protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            CDS exon combination :: BC147658.1 [ECO:0000331]
            RNAseq introns       :: single sample supports all introns
                                    SAMN01164131, SAMN01164139 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            NMD candidate          :: translation inferred from conservation
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-702               AL954390.19        40221-40922         c
            703-1508            AL805910.7         189073-189878       c
            1509-1596           AL805910.7         143451-143538       c
            1597-1839           AL805910.7         141831-142073       c
            1840-1970           AL805910.7         139232-139362       c
            1971-3067           AL805910.7         130246-131342       c
FEATURES             Location/Qualifiers
     source          1..3067
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 83.71 cM"
     gene            1..3067
                     /gene="Ajap1"
                     /gene_synonym="Gm573"
                     /note="adherens junction associated protein 1"
                     /db_xref="GeneID:230959"
                     /db_xref="MGI:MGI:2685419"
     exon            1..702
                     /gene="Ajap1"
                     /gene_synonym="Gm573"
                     /inference="alignment:Splign:2.1.0"
     CDS             674..1912
                     /gene="Ajap1"
                     /gene_synonym="Gm573"
                     /note="likely ortholog of H. sapiens transmembrane protein
                     SHREW1"
                     /codon_start=1
                     /product="adherens junction-associated protein 1
                     precursor"
                     /protein_id="NP_001092769.1"
                     /db_xref="CCDS:CCDS38987.1"
                     /db_xref="GeneID:230959"
                     /db_xref="MGI:MGI:2685419"
                     /translation="MWIQQLLGLSSMSIRWPGRSLGSHAWILIAMLQLAVDFPSCDSL
                     GPGPEFRLLSRPQRPQRLWSLRSGPPTRLPTPAWSPRAARAERAHGPIQMQTPRARRA
                     HRPRDQVATLGPKGGLTKPPAATRSSPSLASATASSSIVTAGAAEHQGLLRRGRRHTH
                     DTEFNDFDFRGGRPTTETEFIAWGPTGDEDALESNTFPGGFGPTTVSILQTRKTTVAT
                     TTTTTAASTATAMTLQTKGVTESLDPWKRTPVGVSTTEPSTSPSSNGKDIQPPRILGE
                     TSGLAVHQIITITVSLIMVIAALITTLVLKNCCAPSGHTRRNSHQRKMNQQEESCQNL
                     TDFTPARVPSSVDIFTAYNETLQCSHECVRASVPVYADETLHSTGEYKSTFNGNRTSS
                     ADRHLIPVAFVSEKWFEISC"
     exon            703..1508
                     /gene="Ajap1"
                     /gene_synonym="Gm573"
                     /inference="alignment:Splign:2.1.0"
     exon            1509..1596
                     /gene="Ajap1"
                     /gene_synonym="Gm573"
                     /inference="alignment:Splign:2.1.0"
     exon            1597..1839
                     /gene="Ajap1"
                     /gene_synonym="Gm573"
                     /inference="alignment:Splign:2.1.0"
     exon            1840..1970
                     /gene="Ajap1"
                     /gene_synonym="Gm573"
                     /inference="alignment:Splign:2.1.0"
     exon            1971..3067
                     /gene="Ajap1"
                     /gene_synonym="Gm573"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3048..3053
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ajap1"
                     /gene_synonym="Gm573"
                     /note="hexamer: ATTAAA"
     polyA_site      3067
                     /gene="Ajap1"
                     /gene_synonym="Gm573"
                     /note="major polyA site"
ORIGIN      
        1 cacgccccgc gctccacgct tcttcgcccc gcgcctcccc tgcccgcctt ctggcagtct
       61 ccgggccgcg gctgtctgca gagcgagcgc ttagacggag ctctccagca acttgatcgg
      121 tgtcagaacc tgggacagag gcccctcgac ccggcggcgc gtggaggcga cccgcgtggg
      181 ccagaggggc gatcttctca acccccgggc aacaccgatc acagcctcgc agctccggct
      241 gcaggctaga tcctcgcacg gcttgcggag ccgcccgctt cgctactcgt cgccccggag
      301 gacgaagccc cgctcctcgg accctcgcgg ccggcgacag acccggagtg cgcccgcctc
      361 gctgccgccc gcgccgtgtg gcctgcggtg ggggccccgc gccccgagag ccggcgggag
      421 gatgtgcgtc cccgcgggcc ggcccgcctg agccatgcgc cccctcggcg gcggcgcgcc
      481 ggccggcatg gagccccgcg cggccgcgct ctgactcgct gtgcgccccg cggccggcgg
      541 gcggcgggcg gcgggtggcg gcggcggccc gaagccgaag agcggcgcca cgggaccgga
      601 gcgagcgggc gcgggcgccg cgcagatggc tggggcaagc aaggtctgag gctgggttcc
      661 ctgaaacgtg accatgtgga tccaacagct tttaggactc agctccatgt ctatccgctg
      721 gcccggccgc tctctcggaa gccatgcctg gatactgata gccatgctgc agcttgcggt
      781 cgacttccca tcctgtgact cactaggccc tgggcccgag ttccggctcc tgtcacgccc
      841 acagaggccc cagcggctgt ggagtctgcg aagtggaccc ccaacgaggc taccgacacc
      901 ggcctggagt ccccgtgctg cccgggcaga acgtgcccac ggacccatac agatgcagac
      961 tccccgtgct cggagggccc acaggcccag ggaccaggta gccaccctcg gacccaaagg
     1021 gggactcacc aagcccccag ctgccaccag gtcgagccct tccctcgcct ctgcgacggc
     1081 ctcatcctcc atagtgactg ctggggctgc agagcaccag ggcctcctaa gacggggtag
     1141 gaggcacaca catgatacag agttcaacga tttcgacttc cgtggtggca ggcccacgac
     1201 agagacagaa ttcatagcct gggggcctac gggggatgag gacgccctgg agtccaacac
     1261 cttccctggt ggtttcggcc ccaccacggt ctccatcttg caaacacgga agacaacggt
     1321 ggccaccacc accaccacca ccgcggccag cacggccacc gccatgacac tgcagactaa
     1381 gggggtcacc gagtccctgg atccctggaa aaggacccct gttggggtta gcacaacgga
     1441 gccttccact agtcccagca gcaatgggaa agacatccag cccccgagga tcttagggga
     1501 gacctcaggt ctggctgtgc atcagatcat caccatcacc gtctccctca tcatggtcat
     1561 cgctgcgctc atcacaactc ttgtcttaaa aaattgctgt gccccaagcg ggcacactcg
     1621 gaggaatagc caccagcgga agatgaatca gcaggaggag agttgccaga acttgacgga
     1681 cttcaccccg gcccgggtgc ccagcagtgt ggacatcttc accgcctata atgagacact
     1741 gcagtgttct cacgagtgtg tcagggcctc tgtgcccgtg tatgccgacg agacgctgca
     1801 ctcgaccggg gagtacaagt ccacattcaa cggaaaccga acatcctctg cggataggca
     1861 tctcatccct gtggcctttg tgtctgagaa atggtttgaa atctcctgct gactggccga
     1921 aggcttttta cctcctgggg gcggggcaga cgccatgtgt ctgtttcatg gattccgttg
     1981 gtgaacttgt acaaacaaaa caacaaaaca aaacaaaaaa attataaaac ctaaacctaa
     2041 actatctaag gggagggtcc cgcacccgct gcttctgttt gcccgcggga actcagctga
     2101 gcaggacacc caaggccaaa tctatttttg taaaattgct cgtgcctagg ggcgctcccg
     2161 tctcccagag ttttctccgg agagcagaaa gaagacacgc aagaagcgga ctggagacgg
     2221 cagagaatgc catgcagaga aggacaggag acagctcccg acgcctggga ctcgcaccac
     2281 gattctgaac ctgtgccaat aataccacta tgtgccatgt aatgacccgg aagctcggca
     2341 ggagccgagc ccagcgaacc acgtggagag atcttaacag aggacagggg tggggatcac
     2401 ttccggtaga gtcactattt ctggttaata tacatatata aatatataaa taccaacaca
     2461 cagacacaca cacttttttt gtactgtagc aatttttgaa gatcttcaat gttccttttt
     2521 aaaaagaaaa agaaaaaaag aattgtgttc tagtcacaaa acttgattta tttaacatgc
     2581 ttagtatgaa cagaaataaa cctgtgtttt gtactttggc aaatcacatc acacgcagca
     2641 cacacacaca tgcacccgca cccgcacccg cacatacaaa catacaagca tttatatgca
     2701 tatattggag aagccgttct gcaggtcgtc cagggagaca gcctcaaccc agaggacgag
     2761 cccttacgtg acaacttcca tccgtcagac acttgccgaa cggtactcat gtgtggcttt
     2821 atcacttggg ccagaattaa gaatgctgtg gttttctctc tgtgttcaca cacacggtca
     2881 gtgctgcatt tcttcattta actgtctgag gatgctaaca gccacacgcg ctcacgcagc
     2941 cgactgccaa cgagtgagcc tgagaaccag gttgggtggg tggcagattc tcgaacattt
     3001 gtgtcataca agcatagact gaactaaaga gattttacag ttccataatt aaaggaatat
     3061 atccgta
//
