LOCUS       NM_175178               2688 bp    mRNA    linear   ROD 12-FEB-2025
DEFINITION  Mus musculus apoptosis-inducing factor, mitochondrion-associated 3
            (Aifm3), transcript variant 2, mRNA.
ACCESSION   NM_175178
VERSION     NM_175178.4
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   Ma,H., Jia,H., Zou,W., Ji,F., Wang,W., Zhao,J., Yuan,C. and Jiao,J.
  TITLE     Gasdermin D Mediated Mitochondrial Metabolism Orchestrate
            Neurogenesis Through LDHA During Embryonic Development
  JOURNAL   Adv Sci (Weinh) 11 (35), e2402285 (2024)
   PUBMED   39033542
REFERENCE   2  (bases 1 to 2688)
  AUTHORS   Vong,K.I., Lee,S., Au,K.S., Crowley,T.B., Capra,V., Martino,J.,
            Haller,M., Araujo,C., Machado,H.R., George,R., Gerding,B.,
            James,K.N., Stanley,V., Jiang,N., Alu,K., Meave,N., Nidhiry,A.S.,
            Jiwani,F., Tang,I., Nisal,A., Jhamb,I., Patel,A., Patel,A.,
            McEvoy-Venneri,J., Barrows,C., Shen,C., Ha,Y.J., Howarth,R.,
            Strain,M., Ashley-Koch,A.E., Azam,M., Mumtaz,S., Bot,G.M.,
            Finnell,R.H., Kibar,Z., Marwan,A.I., Melikishvili,G., Meltzer,H.S.,
            Mutchinick,O.M., Stevenson,D.A., Mroczkowski,H.J., Ostrander,B.,
            Schindewolf,E., Moldenhauer,J., Zackai,E.H., Emanuel,B.S.,
            Garcia-Minaur,S., Nowakowska,B.A., Stevenson,R.E., Zaki,M.S.,
            Northrup,H., McNamara,H.K., Aldinger,K.A., Phelps,I.G., Deng,M.,
            Glass,I.A., Morrow,B., McDonald-McGinn,D.M., Sanna-Cherchi,S.,
            Lamb,D.J. and Gleeson,J.G.
  CONSRTM   Spina Bifida Sequencing Consortiumdouble dagger
  TITLE     Risk of meningomyelocele mediated by the common 22q11.2 deletion
  JOURNAL   Science 384 (6695), 584-590 (2024)
   PUBMED   38696583
REFERENCE   3  (bases 1 to 2688)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   4  (bases 1 to 2688)
  AUTHORS   Nakano,Y., Wiechert,S. and Banfi,B.
  TITLE     Overlapping Activities of Two Neuronal Splicing Factors Switch the
            GABA Effect from Excitatory to Inhibitory by Regulating REST
  JOURNAL   Cell Rep 27 (3), 860-871 (2019)
   PUBMED   30995482
REFERENCE   5  (bases 1 to 2688)
  AUTHORS   Racedo,S.E., McDonald-McGinn,D.M., Chung,J.H., Goldmuntz,E.,
            Zackai,E., Emanuel,B.S., Zhou,B., Funke,B. and Morrow,B.E.
  TITLE     Mouse and human CRKL is dosage sensitive for cardiac outflow tract
            formation
  JOURNAL   Am J Hum Genet 96 (2), 235-244 (2015)
   PUBMED   25658046
REFERENCE   6  (bases 1 to 2688)
  AUTHORS   Chinta,S.J., Rane,A., Yadava,N., Andersen,J.K., Nicholls,D.G. and
            Polster,B.M.
  TITLE     Reactive oxygen species regulation by AIF- and complex I-depleted
            brain mitochondria
  JOURNAL   Free Radic Biol Med 46 (7), 939-947 (2009)
   PUBMED   19280713
  REMARK    GeneRIF: AIF-deficient mice display similar rates of H2O2 release
            and protein carbonyl modifications in brain mitochondria compared
            to WT controls
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY255255.1, AK158809.1 and CD742217.1.
            
            On Mar 22, 2014 this sequence version replaced NM_175178.3.
            
            Transcript Variant: This variant (2) lacks an in-frame alternate
            exon in the 3' coding region, compared to variant 1. It encodes a
            shorter isoform (2), compared to isoform 1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC046517.1, SRR7652917.202054.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849379
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-480               BY255255.1         2-481
            481-2314            AK158809.1         143-1976
            2315-2688           CD742217.1         1-374               c
FEATURES             Location/Qualifiers
     source          1..2688
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="16"
                     /map="16 10.84 cM"
     gene            1..2688
                     /gene="Aifm3"
                     /gene_synonym="2810401C16Rik; Aifl"
                     /note="apoptosis-inducing factor, mitochondrion-associated
                     3"
                     /db_xref="GeneID:72168"
                     /db_xref="MGI:MGI:1919418"
     exon            1..215
                     /gene="Aifm3"
                     /gene_synonym="2810401C16Rik; Aifl"
                     /inference="alignment:Splign:2.1.0"
     exon            216..406
                     /gene="Aifm3"
                     /gene_synonym="2810401C16Rik; Aifl"
                     /inference="alignment:Splign:2.1.0"
     exon            407..589
                     /gene="Aifm3"
                     /gene_synonym="2810401C16Rik; Aifl"
                     /inference="alignment:Splign:2.1.0"
     CDS             559..2355
                     /gene="Aifm3"
                     /gene_synonym="2810401C16Rik; Aifl"
                     /note="isoform 2 is encoded by transcript variant 2;
                     apoptosis-inducing factor 3; apoptosis-inducing factor
                     like; apoptosis-inducing factor-like protein"
                     /codon_start=1
                     /product="apoptosis-inducing factor 3 isoform 2"
                     /protein_id="NP_780387.2"
                     /db_xref="CCDS:CCDS28003.1"
                     /db_xref="GeneID:72168"
                     /db_xref="MGI:MGI:1919418"
                     /translation="MGGCFSKPKPVELKIEVVLPEKERGKEELSASGKGSPRGYQGNG
                     TARHFHAEERLPTPQPYPSPQDCVEATVCHVKDLENGQMREVELGWGKVLLVKDNGEF
                     HALGHKCPHYGAPLVKGVLSRGRVRCPWHGACFNISTGDLEDFPGLDSLHKFQVKIEK
                     EKVTIRASKQALQLQRRTKVMAKCISPSAGHSSSTNVLIVGAGAAGLVCAETLRQEGF
                     SDRIVLCTLDRHLPYDRAKLSKSLDAQPEQLALRPKEFFRAYGIEMLTEAQVVTVDVR
                     NKKVVFKDGFKLEYSKLLLAPGSSPKTLTCKGKDVENVFTIRTPEDANRVLRLARGRN
                     AVVVGAGFLGMEVAAYLTEKAHSVSVVELEETPFRRFLGERVGRALMKMFENNRVKFY
                     MQTEVSELRAQEGKLQEVVLKSSKVLRADVCVLGIGAVPATGFLRQSGIGLDSRGFIP
                     VNKMMQTNVPGVFAAGDAVTFPLAWRNNRKVNIPHWQMAHAQGRVAAQNMLAQEAEIN
                     TVPYLWTAMFGKSLRYAGYGEGFDDVIIQGDLEELKFVAFYTKSDEVIAVASMNYDPI
                     VSKVAEVLASGRAIRKREVETGDMSWLTGKGS"
     exon            590..803
                     /gene="Aifm3"
                     /gene_synonym="2810401C16Rik; Aifl"
                     /inference="alignment:Splign:2.1.0"
     exon            804..913
                     /gene="Aifm3"
                     /gene_synonym="2810401C16Rik; Aifl"
                     /inference="alignment:Splign:2.1.0"
     exon            914..1023
                     /gene="Aifm3"
                     /gene_synonym="2810401C16Rik; Aifl"
                     /inference="alignment:Splign:2.1.0"
     exon            1024..1068
                     /gene="Aifm3"
                     /gene_synonym="2810401C16Rik; Aifl"
                     /inference="alignment:Splign:2.1.0"
     exon            1069..1165
                     /gene="Aifm3"
                     /gene_synonym="2810401C16Rik; Aifl"
                     /inference="alignment:Splign:2.1.0"
     exon            1166..1278
                     /gene="Aifm3"
                     /gene_synonym="2810401C16Rik; Aifl"
                     /inference="alignment:Splign:2.1.0"
     exon            1279..1365
                     /gene="Aifm3"
                     /gene_synonym="2810401C16Rik; Aifl"
                     /inference="alignment:Splign:2.1.0"
     exon            1366..1457
                     /gene="Aifm3"
                     /gene_synonym="2810401C16Rik; Aifl"
                     /inference="alignment:Splign:2.1.0"
     exon            1458..1588
                     /gene="Aifm3"
                     /gene_synonym="2810401C16Rik; Aifl"
                     /inference="alignment:Splign:2.1.0"
     exon            1589..1704
                     /gene="Aifm3"
                     /gene_synonym="2810401C16Rik; Aifl"
                     /inference="alignment:Splign:2.1.0"
     exon            1705..1776
                     /gene="Aifm3"
                     /gene_synonym="2810401C16Rik; Aifl"
                     /inference="alignment:Splign:2.1.0"
     exon            1777..1840
                     /gene="Aifm3"
                     /gene_synonym="2810401C16Rik; Aifl"
                     /inference="alignment:Splign:2.1.0"
     exon            1841..1917
                     /gene="Aifm3"
                     /gene_synonym="2810401C16Rik; Aifl"
                     /inference="alignment:Splign:2.1.0"
     exon            1918..2035
                     /gene="Aifm3"
                     /gene_synonym="2810401C16Rik; Aifl"
                     /inference="alignment:Splign:2.1.0"
     exon            2036..2134
                     /gene="Aifm3"
                     /gene_synonym="2810401C16Rik; Aifl"
                     /inference="alignment:Splign:2.1.0"
     exon            2135..2210
                     /gene="Aifm3"
                     /gene_synonym="2810401C16Rik; Aifl"
                     /inference="alignment:Splign:2.1.0"
     exon            2211..2315
                     /gene="Aifm3"
                     /gene_synonym="2810401C16Rik; Aifl"
                     /inference="alignment:Splign:2.1.0"
     exon            2316..2674
                     /gene="Aifm3"
                     /gene_synonym="2810401C16Rik; Aifl"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2651..2656
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Aifm3"
                     /gene_synonym="2810401C16Rik; Aifl"
                     /note="hexamer: ATTAAA"
     polyA_site      2671
                     /gene="Aifm3"
                     /gene_synonym="2810401C16Rik; Aifl"
                     /note="major polyA site"
ORIGIN      
        1 aggtgagcgc gcctgtccct gtcccgcacg cgccccggga cagctcgctc gcggatttct
       61 accctggtgg cagagtgagg cactcaggag gctgcagcga ccccggtcct tgctgctgct
      121 ccccagcacc cctttcggct ccctggagcc ctccctacct tgtgctggtc ggttggttgg
      181 ctgctgcttc cagcgaggga gccgcaaaga agccgagctg ggtttccctg gggaccagtg
      241 gcttggccta gccccacacc cacagccccc tcctggccac aactcatgct agaaggcggg
      301 ctggaccctc caggacactg gccttccgtg ccctgctgac caaggcccag tagtctctct
      361 ccattgctgg gctgggagca aggagcagtg actcccatgg aaagaggtcc taaagctgtc
      421 tagctggcat ggcagctcta gcgtctctga gggccacagg gggtccagtc ctgcagggca
      481 catcctagac cttggacagc tggacagcct gccatcgtgc tcccaccagc caggccagat
      541 cccaggccac cgttcaccat gggcggctgc ttctccaagc ccaagccagt ggagctcaag
      601 attgaagtgg tgctgccaga gaaagagcgg ggcaaggagg agctgtcagc cagcggaaag
      661 ggcagccccc ggggttacca gggcaacggc acagcacgcc acttccatgc tgaagagcgg
      721 ctgcccaccc cacaacccta tcccagccct caggactgtg tggaggctac tgtctgccat
      781 gtcaaggacc tcgagaatgg ccagatgcgg gaagtggagc tgggctgggg aaaggtgttg
      841 ctggtgaagg acaatgggga attccatgcc ctgggccata agtgtcctca ctatggagcg
      901 cccctggtga aaggtgtact gtcccgggga cgtgtgcgct gcccctggca tggtgcctgc
      961 ttcaacatta gtactgggga cctagaggac ttcccaggcc tggacagcct gcataagttc
     1021 caggtgaaga ttgagaagga gaaggtgacc attcgggcaa gcaagcaggc cctgcagcta
     1081 cagcggagga caaaggtgat ggccaagtgt atctctccaa gtgctggcca cagcagtagc
     1141 accaacgtgc tcatagtggg tgcaggtgct gctggcctag tgtgtgcaga aacactgagg
     1201 caggagggct tctcagaccg gattgtcctt tgcacactgg atcgacatct gccctatgac
     1261 cgggccaagc tcagcaagtc cctggatgca caacccgaac agctggccct gagacccaag
     1321 gaattcttcc gggcctatgg cattgagatg ctcactgagg ctcaggtagt cacagtggac
     1381 gtgagaaata agaaggttgt gttcaaggat ggcttcaagc tagagtacag caagctgctg
     1441 ctggcaccag gaagtagccc taagaccctg acctgcaaag ggaaagatgt agagaatgtg
     1501 ttcaccattc gcacgcccga ggatgccaac cgcgtgctgc ggctcgcccg gggccgcaat
     1561 gctgttgttg tgggagcagg ctttctggga atggaggttg ctgcttatct gactgagaaa
     1621 gcccactcag tatccgtggt agagctggag gagacaccct tccggaggtt cctgggggaa
     1681 cgtgttggtc gtgcacttat gaagatgttt gaaaacaacc gggtgaagtt ctatatgcag
     1741 acagaggtgt cagagttgcg ggctcaagag ggcaagctgc aggaggtggt gctgaagagc
     1801 agcaaagtcc tgcgagcaga tgtctgcgtg ttaggcattg gtgcggtgcc tgccacaggc
     1861 ttcctgaggc agagtggcat tggtctggat tcccgaggtt tcatcccagt caacaagatg
     1921 atgcagacca acgtcccagg agtgtttgct gcaggtgatg ctgtcacctt tcctcttgcc
     1981 tggaggaaca atcggaaagt gaacatccca cactggcaga tggctcacgc ccaggggcgg
     2041 gtagcagctc agaacatgct ggcacaggag gctgagatca acacggtacc ctatctgtgg
     2101 actgccatgt tcggcaaaag ccttcgctat gctggctacg gagaaggctt cgatgatgtc
     2161 atcattcagg gggatctgga ggagctgaag tttgtggctt tttataccaa aagtgacgaa
     2221 gtgattgctg tggccagcat gaactacgat cccatcgtat ccaaggtggc tgaggtgcta
     2281 gcctcaggtc gagccatccg gaagcgggag gtggaaaccg gtgacatgtc ttggctcaca
     2341 ggaaaaggat cctgagctct cctgtggtgg acttgggcag gcctctgggg taccaaggtc
     2401 agagaccaag tcctggggca agtgccaact ccaatttccc aggatccctc actgtggaat
     2461 ctgagtcctc caaggcttgc cttgacctgt ctgcctcgcc tccagccagg ctaccacagc
     2521 ctccagaaga tggtccattc ctaatgcctc agctgtcact gacagctggg tgtagaggca
     2581 ggacaggctc tgccttggtt tcctctacta ggacgatttt cccagcaagc cccttcaaca
     2641 ttaccttaaa attaaaacgc atgtctcagc agaaaaaaaa aaaaaaaa
//
