LOCUS       NM_012019               2249 bp    mRNA    linear   ROD 11-MAR-2025
DEFINITION  Mus musculus apoptosis-inducing factor, mitochondrion-associated 1
            (Aifm1), transcript variant 1, mRNA; nuclear gene for mitochondrial
            product.
ACCESSION   NM_012019
VERSION     NM_012019.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Zhou,M., Liu,S., Wang,Y., Zhang,B., Zhu,M., Wang,J.E., Rajaram,V.,
            Fang,Y., Luo,W. and Wang,Y.
  TITLE     AIF3 splicing variant elicits mitochondrial malfunction via the
            concurrent dysregulation of electron transport chain and
            glutathione-redox homeostasis
  JOURNAL   Nat Commun 16 (1), 1804 (2025)
   PUBMED   39979311
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Shi,T., Chen,Z., Li,J., Wang,H. and Wang,Q.
  TITLE     AIF translocation into nucleus caused by Aifm1 R450Q mutation:
            generation and characterization of a mouse model for AUNX1
  JOURNAL   Hum Mol Genet 33 (10), 905-918 (2024)
   PUBMED   38449065
  REMARK    GeneRIF: AIF translocation into nucleus caused by Aifm1 R450Q
            mutation: generation and characterization of a mouse model for
            AUNX1.
REFERENCE   3  (bases 1 to 2249)
  AUTHORS   Wang,S., Matsumoto,K., Mehlferber,M.M., Zhang,G., Aronova,M.A. and
            Yamada,K.M.
  TITLE     Microtubule-dependent apical polarization of basement membrane
            matrix mRNAs in mouse epithelial cells
  JOURNAL   Cells Dev 177, 203898 (2024)
   PUBMED   38103869
REFERENCE   4  (bases 1 to 2249)
  AUTHORS   Mao,J., Wang,D., Wang,D., Wu,Q., Shang,Q., Gao,C., Wang,H.,
            Wang,H., Du,M., Peng,P., Jia,H., Xu,X., Wang,J., Yang,L. and Luo,Z.
  TITLE     SIRT5-related desuccinylation modification of AIFM1 protects
            against compression-induced intervertebral disc degeneration by
            regulating mitochondrial homeostasis
  JOURNAL   Exp Mol Med 55 (1), 253-268 (2023)
   PUBMED   36653443
  REMARK    GeneRIF: SIRT5-related desuccinylation modification of AIFM1
            protects against compression-induced intervertebral disc
            degeneration by regulating mitochondrial homeostasis.
REFERENCE   5  (bases 1 to 2249)
  AUTHORS   Li,T., Sun,Y., Zhang,S., Xu,Y., Li,K., Xie,C., Wang,Y., Wang,Y.,
            Cao,J., Wang,X., Penninger,J.M., Kroemer,G., Blomgren,K. and Zhu,C.
  TITLE     AIF Overexpression Aggravates Oxidative Stress in Neonatal Male
            Mice After Hypoxia-Ischemia Injury
  JOURNAL   Mol Neurobiol 59 (11), 6613-6631 (2022)
   PUBMED   35974295
  REMARK    GeneRIF: AIF Overexpression Aggravates Oxidative Stress in Neonatal
            Male Mice After Hypoxia-Ischemia Injury.
REFERENCE   6  (bases 1 to 2249)
  AUTHORS   Joza,N., Susin,S.A., Daugas,E., Stanford,W.L., Cho,S.K., Li,C.Y.,
            Sasaki,T., Elia,A.J., Cheng,H.Y., Ravagnan,L., Ferri,K.F.,
            Zamzami,N., Wakeham,A., Hakem,R., Yoshida,H., Kong,Y.Y., Mak,T.W.,
            Zuniga-Pflucker,J.C., Kroemer,G. and Penninger,J.M.
  TITLE     Essential role of the mitochondrial apoptosis-inducing factor in
            programmed cell death
  JOURNAL   Nature 410 (6828), 549-554 (2001)
   PUBMED   11279485
REFERENCE   7  (bases 1 to 2249)
  AUTHORS   Loeffler,M., Daugas,E., Susin,S.A., Zamzami,N., Metivier,D.,
            Nieminen,A.L., Brothers,G., Penninger,J.M. and Kroemer,G.
  TITLE     Dominant cell death induction by extramitochondrially targeted
            apoptosis-inducing factor
  JOURNAL   FASEB J 15 (3), 758-767 (2001)
   PUBMED   11259394
REFERENCE   8  (bases 1 to 2249)
  AUTHORS   Susin,S.A., Daugas,E., Ravagnan,L., Samejima,K., Zamzami,N.,
            Loeffler,M., Costantini,P., Ferri,K.F., Irinopoulou,T.,
            Prevost,M.C., Brothers,G., Mak,T.W., Penninger,J., Earnshaw,W.C.
            and Kroemer,G.
  TITLE     Two distinct pathways leading to nuclear apoptosis
  JOURNAL   J Exp Med 192 (4), 571-580 (2000)
   PUBMED   10952727
REFERENCE   9  (bases 1 to 2249)
  AUTHORS   Daugas,E., Nochy,D., Ravagnan,L., Loeffler,M., Susin,S.A.,
            Zamzami,N. and Kroemer,G.
  TITLE     Apoptosis-inducing factor (AIF): a ubiquitous mitochondrial
            oxidoreductase involved in apoptosis
  JOURNAL   FEBS Lett 476 (3), 118-123 (2000)
   PUBMED   10913597
  REMARK    Review article
REFERENCE   10 (bases 1 to 2249)
  AUTHORS   Susin,S.A., Lorenzo,H.K., Zamzami,N., Marzo,I., Snow,B.E.,
            Brothers,G.M., Mangion,J., Jacotot,E., Costantini,P., Loeffler,M.,
            Larochette,N., Goodlett,D.R., Aebersold,R., Siderovski,D.P.,
            Penninger,J.M. and Kroemer,G.
  TITLE     Molecular characterization of mitochondrial apoptosis-inducing
            factor
  JOURNAL   Nature 397 (6718), 441-446 (1999)
   PUBMED   9989411
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL669901.26, AK133764.1, AF100927.1 and AA261021.1.
            
            On Mar 12, 2014 this sequence version replaced NM_012019.2.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC003292.1, AF100927.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164134, SAMN01164135
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-160               AL669901.26        84822-84981         c
            161-632             AK133764.1         2-473
            633-2106            AF100927.1         459-1932
            2107-2249           AA261021.1         184-326
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="X"
                     /map="X 25.68 cM"
     gene            1..2249
                     /gene="Aifm1"
                     /gene_synonym="AIF; AIFsh2; Hq; Pdcd8"
                     /note="apoptosis-inducing factor, mitochondrion-associated
                     1"
                     /db_xref="GeneID:26926"
                     /db_xref="MGI:MGI:1349419"
     exon            1..328
                     /gene="Aifm1"
                     /gene_synonym="AIF; AIFsh2; Hq; Pdcd8"
                     /inference="alignment:Splign:2.1.0"
     CDS             226..2064
                     /gene="Aifm1"
                     /gene_synonym="AIF; AIFsh2; Hq; Pdcd8"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; apoptosis-inducing factor 1, mitochondrial;
                     programmed cell death protein 8; harlequin"
                     /codon_start=1
                     /product="apoptosis-inducing factor 1, mitochondrial
                     isoform 1 precursor"
                     /protein_id="NP_036149.1"
                     /db_xref="CCDS:CCDS30109.1"
                     /db_xref="GeneID:26926"
                     /db_xref="MGI:MGI:1349419"
                     /translation="MFRCGGLAGAFKQKLVPLVRTVYVQRPKQRNRLPGNLFQQWRVP
                     LELQMARQMASSGSSGGKMDNSVLVLIVGLSTIGAGAYAYKTIKEDQKRYNERVMGLG
                     LSPEEKQRRAIASATEGGSVPQIRAPSHVPFLLIGGGTAAFAAARSIRARDPGARVLI
                     VSEDPELPYMRPPLSKELWFSDDPNVTKTLQFRQWNGKERSIYFQPPSFYVSAQDLPN
                     IENGGVAVLTGKKVVHLDVRGNMVKLNDGSQITFEKCLIATGGTPRSLSAIDRAGAEV
                     KSRTTLFRKIGDFRALEKISREVKSITVIGGGFLGSELACALGRKSQASGIEVIQLFP
                     EKGNMGKILPQYLSNWTMEKVKREGVKVMPNAIVQSVGVSGGRLLIKLKDGRKVETDH
                     IVTAVGLEPNVELAKTGGLEIDSDFGGFRVNAELQARSNIWVAGDAACFYDIKLGRRR
                     VEHHDHAVVSGRLAGENMTGAAKPYWHQSMFWSDLGPDVGYEAIGLVDSSLPTVGVFA
                     KATAQDNPKSATEQSGTGIRSESETESEASEITIPPSAPAVPQVPVEGEDYGKGVIFY
                     LRDKVVVGIVLWNVFNRMPIARKIIKDGEQHEDLNEVAKLFNIHED"
     exon            329..471
                     /gene="Aifm1"
                     /gene_synonym="AIF; AIFsh2; Hq; Pdcd8"
                     /inference="alignment:Splign:2.1.0"
     exon            472..571
                     /gene="Aifm1"
                     /gene_synonym="AIF; AIFsh2; Hq; Pdcd8"
                     /inference="alignment:Splign:2.1.0"
     exon            572..696
                     /gene="Aifm1"
                     /gene_synonym="AIF; AIFsh2; Hq; Pdcd8"
                     /inference="alignment:Splign:2.1.0"
     exon            697..827
                     /gene="Aifm1"
                     /gene_synonym="AIF; AIFsh2; Hq; Pdcd8"
                     /inference="alignment:Splign:2.1.0"
     exon            828..918
                     /gene="Aifm1"
                     /gene_synonym="AIF; AIFsh2; Hq; Pdcd8"
                     /inference="alignment:Splign:2.1.0"
     exon            919..1003
                     /gene="Aifm1"
                     /gene_synonym="AIF; AIFsh2; Hq; Pdcd8"
                     /inference="alignment:Splign:2.1.0"
     exon            1004..1080
                     /gene="Aifm1"
                     /gene_synonym="AIF; AIFsh2; Hq; Pdcd8"
                     /inference="alignment:Splign:2.1.0"
     exon            1081..1189
                     /gene="Aifm1"
                     /gene_synonym="AIF; AIFsh2; Hq; Pdcd8"
                     /inference="alignment:Splign:2.1.0"
     exon            1190..1297
                     /gene="Aifm1"
                     /gene_synonym="AIF; AIFsh2; Hq; Pdcd8"
                     /inference="alignment:Splign:2.1.0"
     exon            1298..1386
                     /gene="Aifm1"
                     /gene_synonym="AIF; AIFsh2; Hq; Pdcd8"
                     /inference="alignment:Splign:2.1.0"
     exon            1387..1527
                     /gene="Aifm1"
                     /gene_synonym="AIF; AIFsh2; Hq; Pdcd8"
                     /inference="alignment:Splign:2.1.0"
     exon            1528..1670
                     /gene="Aifm1"
                     /gene_synonym="AIF; AIFsh2; Hq; Pdcd8"
                     /inference="alignment:Splign:2.1.0"
     exon            1671..1795
                     /gene="Aifm1"
                     /gene_synonym="AIF; AIFsh2; Hq; Pdcd8"
                     /inference="alignment:Splign:2.1.0"
     exon            1796..1992
                     /gene="Aifm1"
                     /gene_synonym="AIF; AIFsh2; Hq; Pdcd8"
                     /inference="alignment:Splign:2.1.0"
     exon            1993..2237
                     /gene="Aifm1"
                     /gene_synonym="AIF; AIFsh2; Hq; Pdcd8"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2217..2222
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Aifm1"
                     /gene_synonym="AIF; AIFsh2; Hq; Pdcd8"
                     /note="hexamer: ATTAAA"
     polyA_site      2237
                     /gene="Aifm1"
                     /gene_synonym="AIF; AIFsh2; Hq; Pdcd8"
                     /note="major polyA site"
ORIGIN      
        1 ctgagtctta cgcctgcgca gtgggtctcc agcctcagtg cctttccccc tggctctagt
       61 gggccggttc ctctatccaa tgtccactcg gggctgggag gaggagcctg cgtagtgtgc
      121 gtgtgacggg agggggagcg gacccgagcc cgtggtattc gacccgttcg acggtgcgtg
      181 gaaggaaaag gaaggagcgg gagcttccga ggagtgatcg ccgaaatgtt ccggtgtgga
      241 ggcctggcgg gtgctttcaa gcagaaactg gtgcccttgg tgcggacggt gtacgtccag
      301 aggccgaaac agaggaaccg gcttccaggc aacttgttcc agcaatggcg tgttcctcta
      361 gaactccaga tggcaagaca aatggctagc tctggttcat cagggggcaa aatggataat
      421 tctgtgttag tccttattgt gggcttatca acaataggag ctggtgcata tgcctacaaa
      481 actataaaag aagaccaaaa aagatacaat gaaagagtga tgggattagg actgtcccca
      541 gaagagaaac agagaagagc cattgcctcc gctacagagg gaggctcagt tcctcagatc
      601 agggcaccaa gtcacgtccc tttcctgctg attggtggag ggactgctgc ttttgcagca
      661 gccagatcca tccgggctcg ggatcctggg gccagggtcc tgattgtatc tgaagaccct
      721 gaactgccat acatgcgacc tcctctttca aaagaattgt ggttttcaga tgatccaaat
      781 gtcacaaaga cactgcaatt cagacagtgg aatggaaaag agagaagcat atatttccag
      841 ccaccttctt tctatgtctc tgctcaggac ctgcctaata ttgagaacgg tggtgtggct
      901 gtcctcactg ggaaaaaggt agtacatctg gatgtaagag gcaacatggt gaaacttaat
      961 gatggctctc agattacctt tgaaaagtgc ttgattgcaa cgggaggcac tccaagaagt
     1021 ctgtctgcca tcgatagggc tggagcagag gtgaagagta gaacaacact tttcaggaag
     1081 attggagatt ttagagcctt ggagaagatc tctcgggagg tcaagtcaat tacagttatc
     1141 ggcgggggct tccttgggag tgagctggcc tgtgctcttg gcagaaagtc tcaagcctcg
     1201 ggcatagaag tgatccagct gttccctgag aaaggaaata tggggaagat ccttcctcaa
     1261 tacctcagca actggaccat ggaaaaagtc aaacgagagg gagtgaaagt gatgcccaat
     1321 gcaattgtac aatcagttgg agtcagcggt ggcaggttac tcattaagct gaaagatgga
     1381 aggaaggtag aaactgacca catagtgaca gctgtgggcc tagagcccaa tgttgagttg
     1441 gccaagactg gcggactgga aatagattcc gattttggtg gcttccgggt aaatgcagaa
     1501 ctccaagcac gttctaacat ctgggtggca ggggatgctg catgcttcta tgatataaag
     1561 ttgggtcgaa ggcgagtaga gcatcatgat catgctgttg tgagtggaag actggctgga
     1621 gaaaacatga ctggagccgc taagccatac tggcatcagt caatgttctg gagtgatttg
     1681 ggtcctgatg tcggctatga agctattggt ctggtggata gtagtttgcc cacagttggt
     1741 gtttttgcaa aagcaactgc acaagacaac ccaaaatctg ccacagagca gtcaggaact
     1801 ggtatccgtt cggagagtga gacagagtca gaagcttcgg aaatcacaat tcctcccagc
     1861 gcccctgcag tcccacaggt ccctgttgaa ggggaggact acggcaaagg tgtcatcttc
     1921 tacctcaggg acaaagttgt ggtggggatt gtgctatgga acgtctttaa ccgaatgcca
     1981 attgcaagga agatcattaa ggacggtgag caacatgaag atctcaatga agtagctaaa
     2041 ctcttcaaca ttcatgaaga ttgaatccca atcgtggaat acacaagcac ttttccatcc
     2101 ctggcgggat gggtggatag aacatttttt ttattcagca tacattttct ttatgtagga
     2161 gcaggaatcg aacaagcctc tgtgaatatt ttcatctgta taaatgcaca tcacaaatta
     2221 aaatctgatt cttttcaaaa aaaaaaaaa
//
