LOCUS       NM_001310735            2202 bp    mRNA    linear   ROD 28-JAN-2025
DEFINITION  Mus musculus alanine-glyoxylate aminotransferase 2 (Agxt2),
            transcript variant 1, mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_001310735 XM_006520067
VERSION     NM_001310735.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Rodionov,R.N., Jarzebska,N., Koay,Y.C., Li,M., Kuhn,M.,
            Bornstein,S.R., Martens-Lobenhoffer,J., Eslam,M., Chen,F.W.,
            Rubets,E., Markov,A.G., Weiss,N., Birkenfeld,A., Schwarz,P.,
            Bode-Boger,S.M., Perakakis,N., O'Sullivan,J.F. and George,J.
  TITLE     Symmetric dimethylguanidino valeric acid, a novel single biomarker
            of hepatic steatosis
  JOURNAL   iScience 27 (12), 111366 (2024)
   PUBMED   39660051
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 2202)
  AUTHORS   Yang,F.M., Shen,L., Fan,D.D., Chen,K.H. and Lee,J.
  TITLE     DMGV Is a Rheostat of T Cell Survival and a Potential Therapeutic
            for Inflammatory Diseases and Cancers
  JOURNAL   Front Immunol 13, 918241 (2022)
   PUBMED   35990633
  REMARK    GeneRIF: DMGV Is a Rheostat of T Cell Survival and a Potential
            Therapeutic for Inflammatory Diseases and Cancers.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2202)
  AUTHORS   Rodionov,R.N., Jarzebska,N., Burdin,D., Todorov,V.,
            Martens-Lobenhoffer,J., Hofmann,A., Kolouschek,A., Cordasic,N.,
            Jacobi,J., Rubets,E., Morawietz,H., O'Sullivan,J.F., Markov,A.G.,
            Bornstein,S.R., Hilgers,K., Maas,R., Pfluecke,C., Chen,Y.,
            Bode-Boger,S.M., Hugo,C.P.M., Hohenstein,B. and Weiss,N.
  TITLE     Overexpression of alanine-glyoxylate aminotransferase 2 protects
            from asymmetric dimethylarginine-induced endothelial dysfunction
            and aortic remodeling
  JOURNAL   Sci Rep 12 (1), 9381 (2022)
   PUBMED   35672381
  REMARK    GeneRIF: Overexpression of alanine-glyoxylate aminotransferase 2
            protects from asymmetric dimethylarginine-induced endothelial
            dysfunction and aortic remodeling.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2202)
  AUTHORS   Burdin,D.V., Kolobov,A.A., Brocker,C., Soshnev,A.A., Samusik,N.,
            Demyanov,A.V., Brilloff,S., Jarzebska,N., Martens-Lobenhoffer,J.,
            Mieth,M., Maas,R., Bornstein,S.R., Bode-Boger,S.M., Gonzalez,F.,
            Weiss,N. and Rodionov,R.N.
  TITLE     Diabetes-linked transcription factor HNF4alpha regulates metabolism
            of endogenous methylarginines and beta-aminoisobutyric acid by
            controlling expression of alanine-glyoxylate aminotransferase 2
  JOURNAL   Sci Rep 6, 35503 (2016)
   PUBMED   27752141
  REMARK    GeneRIF: this study shows that diabetes-linked transcription factor
            HNF4alpha regulates maminotransferase 2etabolism of endogenous
            methylarginines and beta-aminoisobutyric acid by controlling
            expression of alanine-glyoxylate
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2202)
  AUTHORS   Rodionov,R.N., Oppici,E., Martens-Lobenhoffer,J., Jarzebska,N.,
            Brilloff,S., Burdin,D., Demyanov,A., Kolouschek,A., Leiper,J.,
            Maas,R., Cellini,B., Weiss,N. and Bode-Boger,S.M.
  TITLE     A Novel Pathway for Metabolism of the Cardiovascular Risk Factor
            Homoarginine by alanine:glyoxylate aminotransferase 2
  JOURNAL   Sci Rep 6, 35277 (2016)
   PUBMED   27752063
  REMARK    GeneRIF: we experimentally proved the presence of a new pathway of
            homoarginine catabolism - its transamination by AGXT2 with
            formation of GOCA and demonstrated that endogenous AGXT2 is
            required for maintenance of homoarginine levels in mice.
            Publication Status: Online-Only
REFERENCE   7  (bases 1 to 2202)
  AUTHORS   Blancquaert,L., Baba,S.P., Kwiatkowski,S., Stautemas,J., Stegen,S.,
            Barbaresi,S., Chung,W., Boakye,A.A., Hoetker,J.D., Bhatnagar,A.,
            Delanghe,J., Vanheel,B., Veiga-da-Cunha,M., Derave,W. and
            Everaert,I.
  TITLE     Carnosine and anserine homeostasis in skeletal muscle and heart is
            controlled by beta-alanine transamination
  JOURNAL   J Physiol 594 (17), 4849-4863 (2016)
   PUBMED   27062388
REFERENCE   8  (bases 1 to 2202)
  AUTHORS   Martens-Lobenhoffer,J., Rodionov,R.N. and Bode-Boger,S.M.
  TITLE     Probing AGXT2 enzyme activity in mouse tissue by applying stable
            isotope-labeled asymmetric dimethyl arginine as substrate
  JOURNAL   J Mass Spectrom 47 (12), 1594-1600 (2012)
   PUBMED   23280748
  REMARK    GeneRIF: In this work, we describe an assay for the AGXT2 activity
            in mouse liver and kidney tissue. The overall production rates of
            DMGV-d(6) in mice were 195.37 pmol/min/mg total protein in liver
            and 85.21 pmol/min/mg total protein in kidney tissue.
REFERENCE   9  (bases 1 to 2202)
  AUTHORS   Caplin,B., Wang,Z., Slaviero,A., Tomlinson,J., Dowsett,L.,
            Delahaye,M., Salama,A., Wheeler,D.C. and Leiper,J.
  CONSRTM   International Consortium for Blood Pressure Genome-Wide Association
            Studies
  TITLE     Alanine-glyoxylate aminotransferase-2 metabolizes endogenous
            methylarginines, regulates NO, and controls blood pressure
  JOURNAL   Arterioscler Thromb Vasc Biol 32 (12), 2892-2900 (2012)
   PUBMED   23023372
  REMARK    GeneRIF: Alanine-glyoxylate aminotransferase-2 metabolizes
            endogenous methylarginines, regulates NO, and controls blood
            pressure.
REFERENCE   10 (bases 1 to 2202)
  AUTHORS   Thiagarajan,R.D., Georgas,K.M., Rumballe,B.A., Lesieur,E.,
            Chiu,H.S., Taylor,D., Tang,D.T., Grimmond,S.M. and Little,M.H.
  TITLE     Identification of anchor genes during kidney development defines
            ontological relationships, molecular subcompartments and regulatory
            pathways
  JOURNAL   PLoS One 6 (2), e17286 (2011)
   PUBMED   21386911
  REMARK    Publication Status: Online-Only
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AI098750.1 and AK149538.1.
            
            On Jul 17, 2015 this sequence version replaced XM_006520067.2.
            
            Transcript Variant: This variant (1) represents the predominant
            transcript and encodes isoform 1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK149538.1, BC141248.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849385, SAMN00849386
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on conservation,
                                                  expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-36                AI098750.1         1-36
            37-2202             AK149538.1         2-2167
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="15"
                     /map="15 5.35 cM"
     gene            1..2202
                     /gene="Agxt2"
                     /note="alanine-glyoxylate aminotransferase 2"
                     /db_xref="GeneID:268782"
                     /db_xref="MGI:MGI:2146052"
     exon            1..149
                     /gene="Agxt2"
                     /inference="alignment:Splign:2.1.0"
     CDS             65..1606
                     /gene="Agxt2"
                     /EC_number="2.6.1.44"
                     /EC_number="2.6.1.40"
                     /EC_number="2.6.1.18"
                     /note="isoform 1 is encoded by transcript variant 1;
                     alanine--glyoxylate aminotransferase 2, mitochondrial; AGT
                     2; D-AIBAT; beta-ALAAT II; beta-alanine-pyruvate
                     aminotransferase; (R)-3-amino-2-methylpropionate--pyruvate
                     transaminase; D-3-aminoisobutyrate-pyruvate
                     aminotransferase; D-beta-aminoisobutyrate-pyruvate
                     aminotransferase"
                     /codon_start=1
                     /product="alanine--glyoxylate aminotransferase 2,
                     mitochondrial isoform 1"
                     /protein_id="NP_001297664.1"
                     /db_xref="CCDS:CCDS79362.1"
                     /db_xref="GeneID:268782"
                     /db_xref="MGI:MGI:2146052"
                     /translation="MSLAWRNLQKPFYLETSLRILQMRPSLSLGASRIAVPKLTLHTK
                     HSMPPCDFSPEKYQSLAYSRVLAIHKQHLSPVDTAYFRKPLLLHQGHMEWLFDSEGNR
                     YLDFFSGIVTVSVGHCHPKVSAVAKKQIDRLWHTSSVFFHSPMHEYAEKLSALLPEPL
                     KVIFLVNSGSEANDLAMVMARAHSNHTDIISFRGAYHGCSPYTLGLTNVGIYKMEVPG
                     GIGCQSTMCPDVFRGPWGGIHCRDSPVQTVRDCSCAPDCCQAKERYIEQFKDTLNTSV
                     ATSIAGFFAEPIQGVNGVVQYPKEFLKEAFALVRERGGVCIADEVQTGFGRLGSHFWG
                     FQTHDVLPDIVTMAKGIGNGFPMAAVVTTPEIAKSLAKRLLHFSTFGGNPLACAIGSA
                     VLEVIEEENLQRNSQEVGTYMLLKFAKLRDEFDIVGDVRGKGLMVGIEMVQDKISRQP
                     LPKTEVNQIHEDCKDMGLLVGRGGNFSQTFRIVPPMCVTKMEVDFAYEVFRAALIQHM
                     ERRAK"
     exon            150..238
                     /gene="Agxt2"
                     /inference="alignment:Splign:2.1.0"
     exon            239..423
                     /gene="Agxt2"
                     /inference="alignment:Splign:2.1.0"
     exon            424..547
                     /gene="Agxt2"
                     /inference="alignment:Splign:2.1.0"
     exon            548..642
                     /gene="Agxt2"
                     /inference="alignment:Splign:2.1.0"
     exon            643..736
                     /gene="Agxt2"
                     /inference="alignment:Splign:2.1.0"
     exon            737..830
                     /gene="Agxt2"
                     /inference="alignment:Splign:2.1.0"
     exon            831..931
                     /gene="Agxt2"
                     /inference="alignment:Splign:2.1.0"
     exon            932..1024
                     /gene="Agxt2"
                     /inference="alignment:Splign:2.1.0"
     exon            1025..1157
                     /gene="Agxt2"
                     /inference="alignment:Splign:2.1.0"
     exon            1158..1249
                     /gene="Agxt2"
                     /inference="alignment:Splign:2.1.0"
     exon            1250..1399
                     /gene="Agxt2"
                     /inference="alignment:Splign:2.1.0"
     exon            1400..1498
                     /gene="Agxt2"
                     /inference="alignment:Splign:2.1.0"
     exon            1499..2202
                     /gene="Agxt2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1888..1893
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Agxt2"
                     /note="hexamer: AATACA"
     polyA_site      1911
                     /gene="Agxt2"
ORIGIN      
        1 agcatttgca gcccctcctc ccgctgacct gcttccacag gattttcttt ctgcctggga
       61 agagatgtct ctagcctgga gaaatttgca gaaacccttt tatctggaga cttctctcag
      121 aatactccag atgcgtccat ccctgagctt aggtgcttcc cggatagctg tgcccaagct
      181 cactcttcat acaaagcaca gcatgccacc gtgtgacttc tcacctgaga aataccagtc
      241 cctggcctac agccgtgtgc tggcgattca caagcagcat ctttctcctg tggatacagc
      301 ctatttccgg aagcctttgc tactccacca gggacacatg gagtggctct ttgattctga
      361 gggaaacaga tacctggact tcttctctgg gattgtcact gttagtgttg gtcactgcca
      421 cccgaaggta agtgcagtgg caaaaaaaca gatagaccgc ctgtggcata caagttctgt
      481 cttcttccac tctccgatgc atgaatatgc agagaagctc tcagcactcc ttcctgagcc
      541 tcttaaggtc attttcttgg tgaacagtgg atcagaagcc aacgacctgg ccatggtgat
      601 ggcccgagca cactcgaatc acacagacat catatctttc agaggagcct atcacggatg
      661 cagtccctat acacttggcc tgacaaatgt cggaatctac aagatggaag tccctggtgg
      721 gataggctgc caatcaacaa tgtgcccaga tgttttccgt ggcccttggg gaggaatcca
      781 ttgtcgagat tctccagtgc aaacagtgag ggactgcagc tgtgctccag actgctgcca
      841 agctaaagaa cggtatatcg aacaattcaa agacacactg aacacttctg tggccacatc
      901 aattgctgga ttttttgcag agccaattca aggtgtgaat ggtgttgtcc agtacccaaa
      961 ggagtttctg aaggaagcct ttgcgttggt ccgagagcgg ggaggtgtgt gcatcgcaga
     1021 tgaagtgcag acaggctttg gcaggctggg ctctcacttc tggggtttcc agacacatga
     1081 tgtacttcct gacattgtca ccatggctaa agggattgga aatggcttcc ccatggctgc
     1141 agttgtgacc actccagaaa ttgcgaagtc tttggctaaa cgtttgcttc acttcagcac
     1201 atttggagga aatcccctgg cctgcgccat tggatctgct gtgcttgagg tgattgaaga
     1261 agaaaatctc cagagaaaca gtcaagaagt tggcacctac atgctgctga agtttgctaa
     1321 gcttcgggac gaatttgata tcgttgggga tgtccgaggc aaaggcctta tggtggggat
     1381 agagatggtt caagacaaga taagccgaca acctcttcct aaaacagaag tcaatcaaat
     1441 ccatgaggac tgcaaagaca tggggctcct agtgggtaga ggtggtaatt tttctcagac
     1501 ttttcgaatt gtgcccccga tgtgtgtcac gaagatggaa gtagattttg catatgaagt
     1561 atttcgagct gccttaattc aacacatgga gagaagagct aagtaagaaa agccatgagc
     1621 ctaaagaacg tctcacctgt gtacagagat gaacttgagg aatgttgaaa aaccacaagc
     1681 attagattaa aaaactgcag ccctctgtag taattgtaaa agacatgacc gactatctat
     1741 ctactgaccg tatttgtcaa cggagccccc attctcctga gaattccatc cttcagggga
     1801 agtatctatc actggctcac agagtaagtc tcagtttgtg tagattagtc tgggctaatg
     1861 ttatttccca ttatatcaac tggtttgaat acacagtata tgtgtctccc atgagtcgtg
     1921 aaggaaactc tttctgtgcc actacctctg aagaaacatt tcttcaggct tcagctcaaa
     1981 tgcctcttgt cacactggaa attgtgtgga aaatgatggg gtttctcact tcacaccagg
     2041 catctggaag ccatgaagat aaagcctagt cactatgcta catctggaaa gtggtcggaa
     2101 gctgagtggt tggtaatgtc aatgagtcac tgaccatgaa tcctggagcc accctacctc
     2161 agggtttctt gatttatgaa ataataaatt catggtgatg ct
//
