LOCUS       NM_016702               1553 bp    mRNA    linear   ROD 01-JUN-2024
DEFINITION  Mus musculus alanine-glyoxylate aminotransferase (Agxt), transcript
            variant 1, mRNA.
ACCESSION   NM_016702 XM_981336
VERSION     NM_016702.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Torella,L., Klermund,J., Bilbao-Arribas,M., Tamayo,I., Andrieux,G.,
            Chmielewski,K.O., Vales,A., Olague,C., Moreno-Luqui,D.,
            Raimondi,I., Abad,A., Torrens-Baile,J., Salido,E., Huarte,M.,
            Hernaez,M., Boerries,M., Cathomen,T., Zabaleta,N. and
            Gonzalez-Aseguinolaza,G.
  TITLE     Efficient and safe therapeutic use of paired Cas9-nickases for
            primary hyperoxaluria type 1
  JOURNAL   EMBO Mol Med 16 (1), 112-131 (2024)
   PUBMED   38182795
REFERENCE   2  (bases 1 to 1553)
  AUTHORS   Martinez-Turrillas,R., Martin-Mallo,A., Rodriguez-Diaz,S.,
            Zapata-Linares,N., Rodriguez-Marquez,P., San Martin-Uriz,P.,
            Vilas-Zornoza,A., Calleja-Cervantes,M.E., Salido,E., Prosper,F. and
            Rodriguez-Madoz,J.R.
  TITLE     In vivo CRISPR-Cas9 inhibition of hepatic LDH as treatment of
            primary hyperoxaluria
  JOURNAL   Mol Ther Methods Clin Dev 25, 137-146 (2022)
   PUBMED   35402636
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1553)
  AUTHORS   Gianmoena,K., Gasparoni,N., Jashari,A., Gabrys,P., Grgas,K.,
            Ghallab,A., Nordstrom,K., Gasparoni,G., Reinders,J., Edlund,K.,
            Godoy,P., Schriewer,A., Hayen,H., Hudert,C.A., Damm,G.,
            Seehofer,D., Weiss,T.S., Boor,P., Anders,H.J., Motrapu,M.,
            Jansen,P., Schiergens,T.S., Falk-Paulsen,M., Rosenstiel,P.,
            Lisowski,C., Salido,E., Marchan,R., Walter,J., Hengstler,J.G. and
            Cadenas,C.
  TITLE     Epigenomic and transcriptional profiling identifies impaired
            glyoxylate detoxification in NAFLD as a risk factor for
            hyperoxaluria
  JOURNAL   Cell Rep 36 (8), 109526 (2021)
   PUBMED   34433051
REFERENCE   4  (bases 1 to 1553)
  AUTHORS   Liu,Y., Zhao,Y., Shukha,Y., Lu,H., Wang,L., Liu,Z., Liu,C.,
            Zhao,Y., Wang,H., Zhao,G., Liang,W., Fan,Y., Chang,L., Yurdagul,A.
            Jr., Pattillo,C.B., Orr,A.W., Aviram,M., Wen,B.,
            Garcia-Barrio,M.T., Zhang,J., Liu,W., Sun,D., Hayek,T., Chen,Y.E.
            and Rom,O.
  TITLE     Dysregulated oxalate metabolism is a driver and therapeutic target
            in atherosclerosis
  JOURNAL   Cell Rep 36 (4), 109420 (2021)
   PUBMED   34320345
REFERENCE   5  (bases 1 to 1553)
  AUTHORS   Rom,O., Liu,Y., Liu,Z., Zhao,Y., Wu,J., Ghrayeb,A., Villacorta,L.,
            Fan,Y., Chang,L., Wang,L., Liu,C., Yang,D., Song,J., Rech,J.C.,
            Guo,Y., Wang,H., Zhao,G., Liang,W., Koike,Y., Lu,H., Koike,T.,
            Hayek,T., Pennathur,S., Xi,C., Wen,B., Sun,D., Garcia-Barrio,M.T.,
            Aviram,M., Gottlieb,E., Mor,I., Liu,W., Zhang,J. and Chen,Y.E.
  TITLE     Glycine-based treatment ameliorates NAFLD by modulating fatty acid
            oxidation, glutathione synthesis, and the gut microbiome
  JOURNAL   Sci Transl Med 12 (572) (2020)
   PUBMED   33268508
REFERENCE   6  (bases 1 to 1553)
  AUTHORS   Hernandez-Fernaud,J.R. and Salido,E.
  TITLE     Differential expression of liver and kidney proteins in a mouse
            model for primary hyperoxaluria type I
  JOURNAL   FEBS J 277 (22), 4766-4774 (2010)
   PUBMED   20977670
REFERENCE   7  (bases 1 to 1553)
  AUTHORS   Salido,E.C., Li,X.M., Lu,Y., Wang,X., Santana,A., Roy-Chowdhury,N.,
            Torres,A., Shapiro,L.J. and Roy-Chowdhury,J.
  TITLE     Alanine-glyoxylate aminotransferase-deficient mice, a model for
            primary hyperoxaluria that responds to adenoviral gene transfer
  JOURNAL   Proc Natl Acad Sci U S A 103 (48), 18249-18254 (2006)
   PUBMED   17110443
  REMARK    GeneRIF: expressed wild-type human AGT1 was predominantly localized
            in mouse hepatocellular peroxisomes, whereas the most common mutant
            form of AGT1 (G170R) was localized predominantly in the
            mitochondria
REFERENCE   8  (bases 1 to 1553)
  AUTHORS   Mootha,V.K., Bunkenborg,J., Olsen,J.V., Hjerrild,M.,
            Wisniewski,J.R., Stahl,E., Bolouri,M.S., Ray,H.N., Sihag,S.,
            Kamal,M., Patterson,N., Lander,E.S. and Mann,M.
  TITLE     Integrated analysis of protein composition, tissue diversity, and
            gene regulation in mouse mitochondria
  JOURNAL   Cell 115 (5), 629-640 (2003)
   PUBMED   14651853
REFERENCE   9  (bases 1 to 1553)
  AUTHORS   Li,X.M., Salido,E.C. and Shapiro,L.J.
  TITLE     The mouse alanine:glyoxylate aminotransferase gene (Agxt1):
            cloning, expression, and mapping to chromosome 1
  JOURNAL   Somat Cell Mol Genet 25 (2), 67-77 (1999)
   PUBMED   11225057
REFERENCE   10 (bases 1 to 1553)
  AUTHORS   Danpure,C.J., Fryer,P., Jennings,P.R., Allsop,J., Griffiths,S. and
            Cunningham,A.
  TITLE     Evolution of alanine:glyoxylate aminotransferase 1 peroxisomal and
            mitochondrial targeting. A survey of its subcellular distribution
            in the livers of various representatives of the classes Mammalia,
            Aves and Amphibia
  JOURNAL   Eur J Cell Biol 64 (2), 295-313 (1994)
   PUBMED   7813517
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BB868742.1, AK149432.1 and AC110247.9.
            
            On Feb 26, 2013 this sequence version replaced NM_016702.2.
            
            Transcript Variant: This variant (1) includes an upstream AUG start
            codon (uAUG) and a downstream AUG start codon (dAUG). Isoform 1
            precursor is encoded from the CDS with the uAUG start codon. The
            mature peptide is located in mitochondrion but the sequence is
            identical to the peroxisomal enzyme (isoform 2).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC025799.1, AK149432.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849386, SAMN01164135
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-78                BB868742.1         2-79
            79-1544             AK149432.1         2-1467
            1545-1553           AC110247.9         6062-6070           c
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 47.0 cM"
     gene            1..1553
                     /gene="Agxt"
                     /gene_synonym="AGT; Agt1; Agxt1; SPT"
                     /note="alanine-glyoxylate aminotransferase"
                     /db_xref="GeneID:11611"
                     /db_xref="MGI:MGI:1329033"
     exon            1..285
                     /gene="Agxt"
                     /gene_synonym="AGT; Agt1; Agxt1; SPT"
                     /inference="alignment:Splign:2.1.0"
     CDS             55..1299
                     /gene="Agxt"
                     /gene_synonym="AGT; Agt1; Agxt1; SPT"
                     /EC_number="2.6.1.44"
                     /EC_number="2.6.1.51"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; serine-pyruvate aminotransferase;
                     alanine-glyoxylate aminotransferase 1; serine--pyruvate
                     aminotransferase, mitochondrial; serine--pyruvate
                     aminotransferase, peroxisomal"
                     /codon_start=1
                     /product="alanine--glyoxylate aminotransferase isoform 1
                     precursor"
                     /protein_id="NP_057911.2"
                     /db_xref="CCDS:CCDS15184.1"
                     /db_xref="GeneID:11611"
                     /db_xref="MGI:MGI:1329033"
                     /translation="MFRMLAKASVTLGSRAAGWVRTMGSYQLLVPPPEALSKPLSVPT
                     RLLLGPGPSNLAPRVLAAGSLRMIGHMQKEMLQIMEEIKQGIQYVFQTRNPLTLVVSG
                     SGHCAMETALFNLLEPGDSFLTGTNGIWGMRAAEIADRIGARVHQMIKKPGEHYTLQE
                     VEEGLAQHKPVLLFLVHGESSTGVVQPLDGFGELCHRYQCLLLVDSVASLGGVPIYMD
                     QQGIDIMYSSSQKVLNAPPGISLISFNDKAKYKVYSRKTKPVSFYTDITYLAKLWGCE
                     GETRVIHHTTPVTSLYCLRESLALIAEQGLENCWRRHREATAHLHKHLQEMGLKFFVK
                     DPEIRLPTITTVTVPAGYNWRDIVSYVLDHFSIEISGGLGPTEERVLRIGLLGYNATT
                     ENVDRVAEALREALQHCPKNKL"
     exon            286..478
                     /gene="Agxt"
                     /gene_synonym="AGT; Agt1; Agxt1; SPT"
                     /inference="alignment:Splign:2.1.0"
     exon            479..543
                     /gene="Agxt"
                     /gene_synonym="AGT; Agt1; Agxt1; SPT"
                     /inference="alignment:Splign:2.1.0"
     exon            544..644
                     /gene="Agxt"
                     /gene_synonym="AGT; Agt1; Agxt1; SPT"
                     /inference="alignment:Splign:2.1.0"
     exon            645..715
                     /gene="Agxt"
                     /gene_synonym="AGT; Agt1; Agxt1; SPT"
                     /inference="alignment:Splign:2.1.0"
     exon            716..800
                     /gene="Agxt"
                     /gene_synonym="AGT; Agt1; Agxt1; SPT"
                     /inference="alignment:Splign:2.1.0"
     exon            801..896
                     /gene="Agxt"
                     /gene_synonym="AGT; Agt1; Agxt1; SPT"
                     /inference="alignment:Splign:2.1.0"
     exon            897..966
                     /gene="Agxt"
                     /gene_synonym="AGT; Agt1; Agxt1; SPT"
                     /inference="alignment:Splign:2.1.0"
     exon            967..1062
                     /gene="Agxt"
                     /gene_synonym="AGT; Agt1; Agxt1; SPT"
                     /inference="alignment:Splign:2.1.0"
     exon            1063..1191
                     /gene="Agxt"
                     /gene_synonym="AGT; Agt1; Agxt1; SPT"
                     /inference="alignment:Splign:2.1.0"
     exon            1192..1553
                     /gene="Agxt"
                     /gene_synonym="AGT; Agt1; Agxt1; SPT"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1527..1532
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Agxt"
                     /gene_synonym="AGT; Agt1; Agxt1; SPT"
                     /note="hexamer: AATAAA"
     polyA_site      1546
                     /gene="Agxt"
                     /gene_synonym="AGT; Agt1; Agxt1; SPT"
                     /note="major polyA site"
ORIGIN      
        1 agggactcat caaccaggcc tggcctctga gttcaacgca gagctagctg ggaaatgttc
       61 cggatgttgg ccaaggccag tgtgacgctg ggctccagag cggcaggttg ggtccggacc
      121 atgggctcgt atcagctgct ggtgccaccg ccagaggccc tgagcaagcc cctgtcagtt
      181 cctacgaggc tcctgttggg tccggggccc tccaacctgg ctccccgtgt gctggcggcg
      241 ggaagtctga gaatgattgg ccacatgcaa aaagagatgc ttcagatcat ggaggagatc
      301 aagcaaggca tccagtatgt gttccagacc aggaaccccc tcacactggt tgtcagcggt
      361 tcagggcact gcgccatgga gactgccctg ttcaacctcc tagagcctgg ggactccttt
      421 ctaaccggaa ccaatggcat ctggggaatg cgggctgcag agattgctga tcgcattgga
      481 gcccgtgtgc accagatgat caagaagcct ggagaacatt atacactgca ggaggtggag
      541 gagggcctgg cccaacacaa accggtattg ctgttcctgg tccacgggga gtcatccact
      601 ggcgtggtgc agcccctgga tggttttggg gagctctgcc ataggtacca gtgcctactc
      661 ctggtggact cagtggcatc cctgggcggg gttcctatct acatggacca acaaggcatt
      721 gacatcatgt actctagctc tcagaaggtc ctgaatgccc caccagggat ctccctcatc
      781 tccttcaacg acaaggccaa atacaaggtc tactcccgga agacaaagcc agtctccttc
      841 tacacagaca tcacatactt ggccaaattg tggggatgtg agggcgagac cagagtaatt
      901 catcatacca cacctgtcac cagcttatac tgcctgaggg agagcctggc actcattgca
      961 gagcagggcc tggagaattg ctggcggcgt cacagggagg ctacagcaca tctgcacaaa
     1021 cacctgcagg agatgggctt aaagttcttt gtgaaggatc cggaaatccg gctacccaca
     1081 atcaccaccg tgactgtgcc tgccggctac aactggaggg acatcgtcag ctatgtgctg
     1141 gaccacttca gcattgaaat ctctgggggt cttgggccca ctgaggagag ggtgctacgg
     1201 attggcctcc tgggctacaa cgccaccact gagaatgtgg accgtgtggc cgaggccctg
     1261 agggaggccc tgcagcattg tcctaagaac aagttgtgag catcctctca ccagactatg
     1321 ccctcctgga ggggctggga atatagcaag aacgaaaaga ctgtgcaagg cctagagcca
     1381 gcaaagatgc tgatgtagcc aggccatgcc ggaaggagca gggtgaagct tcccctctcc
     1441 ctacaaatgg aaccttgtgg aaacaggatg ctaaacacct tctgatggag ctgttgcctg
     1501 caggccactg gtctttggga attttcaata aagtgcttgc gaggaatctc cta
//
