LOCUS       NM_021604               7379 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Mus musculus agrin (Agrn), transcript variant 1, mRNA.
ACCESSION   NM_021604 XM_355545
VERSION     NM_021604.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 7379)
  AUTHORS   Tu,W.Y., Xu,W., Bai,L., Liu,J., Han,Y., Luo,B., Wang,B., Zhang,K.
            and Shen,C.
  TITLE     Local protein synthesis at neuromuscular synapses is required for
            motor functions
  JOURNAL   Cell Rep 43 (9), 114661 (2024)
   PUBMED   39178112
REFERENCE   2  (bases 1 to 7379)
  AUTHORS   Patel,V.N., Aure,M.H., Choi,S.H., Ball,J.R., Lane,E.D., Wang,Z.,
            Xu,Y., Zheng,C., Liu,X., Martin,D., Pailin,J.Y., Prochazkova,M.,
            Kulkarni,A.B., van Kuppevelt,T.H., Ambudkar,I.S., Liu,J. and
            Hoffman,M.P.
  TITLE     Specific 3-O-sulfated heparan sulfate domains regulate salivary
            gland basement membrane metabolism and epithelial differentiation
  JOURNAL   Nat Commun 15 (1), 7584 (2024)
   PUBMED   39217171
  REMARK    Erratum:[Nat Commun. 2024 Oct 10;15(1):8784. doi:
            10.1038/s41467-024-53230-4.. PMID: 39389981]
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 7379)
  AUTHORS   Mueller,J.L., Stavely,R., Guyer,R.A., Soos,A., Bhave,S., Han,C.,
            Hotta,R., Nagy,N. and Goldstein,A.M.
  TITLE     Agrin Inhibition in Enteric Neural Stem Cells Enhances Their
            Migration Following Colonic Transplantation
  JOURNAL   Stem Cells Transl Med 13 (5), 490-504 (2024)
   PUBMED   38387006
  REMARK    GeneRIF: Agrin Inhibition in Enteric Neural Stem Cells Enhances
            Their Migration Following Colonic Transplantation.
REFERENCE   4  (bases 1 to 7379)
  AUTHORS   Schellino,R., Boido,M., Vrijbloed,J.W., Fariello,R.G. and
            Vercelli,A.
  TITLE     Synergistically Acting on Myostatin and Agrin Pathways Increases
            Neuromuscular Junction Stability and Endurance in Old Mice
  JOURNAL   Aging Dis 15 (2), 893-910 (2024)
   PUBMED   37548943
  REMARK    GeneRIF: Synergistically Acting on Myostatin and Agrin Pathways
            Increases Neuromuscular Junction Stability and Endurance in Old
            Mice.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 7379)
  AUTHORS   Chen,J., Chen,H., Dong,X., Hui,T., Yan,M., Ren,D., Zou,S., Wang,S.,
            Fei,E., Zhang,W. and Lai,X.
  TITLE     Deficiency of skeletal muscle Agrin contributes to the pathogenesis
            of age-related sarcopenia in mice
  JOURNAL   Cell Death Dis 15 (3), 201 (2024)
   PUBMED   38461287
  REMARK    GeneRIF: Deficiency of skeletal muscle Agrin contributes to the
            pathogenesis of age-related sarcopenia in mice.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 7379)
  AUTHORS   Lennon,G., Auffray,C., Polymeropoulos,M. and Soares,M.B.
  TITLE     The I.M.A.G.E. Consortium: an integrated molecular analysis of
            genomes and their expression
  JOURNAL   Genomics 33 (1), 151-152 (1996)
   PUBMED   8617505
REFERENCE   7  (bases 1 to 7379)
  AUTHORS   Noakes,P.G., Miner,J.H., Gautam,M., Cunningham,J.M., Sanes,J.R. and
            Merlie,J.P.
  TITLE     The renal glomerulus of mice lacking s-laminin/laminin beta 2:
            nephrosis despite molecular compensation by laminin beta 1
  JOURNAL   Nat Genet 10 (4), 400-406 (1995)
   PUBMED   7670489
REFERENCE   8  (bases 1 to 7379)
  AUTHORS   Skarnes,W.C., Moss,J.E., Hurtley,S.M. and Beddington,R.S.
  TITLE     Capturing genes encoding membrane and secreted proteins important
            for mouse development
  JOURNAL   Proc Natl Acad Sci U S A 92 (14), 6592-6596 (1995)
   PUBMED   7604039
REFERENCE   9  (bases 1 to 7379)
  AUTHORS   Gee,S.H., Montanaro,F., Lindenbaum,M.H. and Carbonetto,S.
  TITLE     Dystroglycan-alpha, a dystrophin-associated glycoprotein, is a
            functional agrin receptor
  JOURNAL   Cell 77 (5), 675-686 (1994)
   PUBMED   8205617
REFERENCE   10 (bases 1 to 7379)
  AUTHORS   Rupp,F., Ozcelik,T., Linial,M., Peterson,K., Francke,U. and
            Scheller,R.
  TITLE     Structure and chromosomal localization of the mammalian agrin gene
  JOURNAL   J Neurosci 12 (9), 3535-3544 (1992)
   PUBMED   1326608
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CT998563.5 and AL928667.4.
            
            On Aug 25, 2011 this sequence version replaced NM_021604.2.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments. The combination of the first three
            exons is inferred based on available transcript data and further
            supported by similar exon-intron structure of the related human
            gene, GeneID 375790.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660813.252955.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164139, SAMN01164142
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-275               CT998563.5         21769-22043         c
            276-537             CT998563.5         19799-20060         c
            538-585             AL928667.4         120281-120328       c
            586-801             AL928667.4         114586-114801       c
            802-1026            AL928667.4         114291-114515       c
            1027-1251           AL928667.4         113976-114200       c
            1252-1458           AL928667.4         113622-113828       c
            1459-1677           AL928667.4         112416-112634       c
            1678-1872           AL928667.4         112149-112343       c
            1873-2073           AL928667.4         111827-112027       c
            2074-2222           AL928667.4         111597-111745       c
            2223-2328           AL928667.4         111412-111517       c
            2329-2445           AL928667.4         110293-110409       c
            2446-2610           AL928667.4         110049-110213       c
            2611-2754           AL928667.4         109764-109907       c
            2755-2879           AL928667.4         109549-109673       c
            2880-2985           AL928667.4         109370-109475       c
            2986-3291           AL928667.4         108978-109283       c
            3292-3429           AL928667.4         108736-108873       c
            3430-3557           AL928667.4         108196-108323       c
            3558-3672           AL928667.4         107961-108075       c
            3673-3792           AL928667.4         107755-107874       c
            3793-4140           AL928667.4         107268-107615       c
            4141-4333           AL928667.4         106981-107173       c
            4334-4549           AL928667.4         106595-106810       c
            4550-4779           AL928667.4         105994-106223       c
            4780-4914           AL928667.4         105772-105906       c
            4915-5011           AL928667.4         105508-105604       c
            5012-5176           AL928667.4         105259-105423       c
            5177-5288           AL928667.4         104963-105074       c
            5289-5405           AL928667.4         104413-104529       c
            5406-5598           AL928667.4         104096-104288       c
            5599-5686           AL928667.4         103926-104013       c
            5687-5911           AL928667.4         102475-102699       c
            5912-6015           AL928667.4         102104-102207       c
            6016-7379           AL928667.4         100483-101846       c
FEATURES             Location/Qualifiers
     source          1..7379
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 88.55 cM"
     gene            1..7379
                     /gene="Agrn"
                     /gene_synonym="Agrin; nmf380"
                     /note="agrin"
                     /db_xref="GeneID:11603"
                     /db_xref="MGI:MGI:87961"
     exon            1..275
                     /gene="Agrn"
                     /gene_synonym="Agrin; nmf380"
                     /inference="alignment:Splign:2.1.0"
     CDS             69..6173
                     /gene="Agrn"
                     /gene_synonym="Agrin; nmf380"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1"
                     /codon_start=1
                     /product="agrin isoform 1 precursor"
                     /protein_id="NP_067617.3"
                     /db_xref="CCDS:CCDS19060.2"
                     /db_xref="GeneID:11603"
                     /db_xref="MGI:MGI:87961"
                     /translation="MVRPRLSFPAPLLPLLLLLAAAAPAVPGASGTCPERALERREEE
                     ANVVLTGTVEEILNVDPVQHTYSCKVRVWRYLKGKDVVAQESLLDGGNKVVIGGFGDP
                     LICDNQVSTGDTRIFFVNPAPPYLWPAHKNELMLNSSLMRITLRNLEEVEFCVEDKPG
                     IHFTAAPSMPPDVCRGMLCGFGAVCEPSVEDPGRASCVCKKNVCPAMVAPVCGSDAST
                     YSNECELQRAQCNQQRRIRLLRQGPCGSRDPCANVTCSFGSTCVPSADGQTASCLCPT
                     TCFGAPDGTVCGSDGVDYPSECQLLRHACANQEHIFKKFDGPCDPCQGSMSDLNHICR
                     VNPRTRHPEMLLRPENCPAQHTPICGDDGVTYENDCVMSRIGAARGLLLQKVRSGQCQ
                     TRDQCPETCQFNSVCLSRRGRPHCSCDRVTCDGAYRPVCAQDGHTYDNDCWRQQAECR
                     QQQTIPPKHQGPCDQTPSPCRGAQCAFGATCTVKNGKAVCECQRVCSGGYDPVCGSDG
                     VTYGSVCELESMACTLGREIRVARRGPCDRCGQCRFGSLCEVETGRCVCPSECVESAQ
                     PVCGSDGHTYASECELHVHACTHQISLYVASAGHCQTCGETVCTFGAVCSAGQCVCPR
                     CEHPPPGPVCGSDGVTYLSACELREAACQQQVQIEEARAGPCEPAECGSGGSGSGEDN
                     ACEQELCRQHGGVWDEDSEDGPCVCDFSCQSVLKSPVCGSDGVTYSTECHLKKARCEA
                     RQELYVAAQGACRGPTLAPLLPMASPHCAQTPYGCCQDNVTAAQGVGLAGCPSTCHCN
                     PHGSYSGTCDPVTGQCSCRPGVGGLRCDRCEPGFWNFRGIVTDGHSGCTPCSCDPRGA
                     VRDDCEQMTGLCSCRPGVAGPKCGQCPDGQALGHLGCEADPTTPVTCVEMHCEFGASC
                     VEEAGFAQCVCPTLTCPEANSTKVCGSDGVTYGNECQLKTIACRQRLDISIQSLGPCR
                     ESVAPGVSPTSASMTTPRHILSRTLASPHSSLPLSPSTTAHDWPTPLPTSPQTVVGTP
                     RSTAATPSDVASLATAIFRESGSTNGSGDEELSGDEEASGGGSGGLEPPVGSVVVTHG
                     PPIERASCYNSPLGCCSDGKTPSLDSEGSNCPATKAFQGVLELEGVEGQELFYTPEMA
                     DPKSELFGETARSIESTLDDLFRNSDVKKDFWSIRLRELGPGKLVRAIVDVHFDPTTA
                     FQAPDVGQALLQQIQVSRPWALAVRRPLREHVRFLDFDWFPTFFTGAATGTTAAVATA
                     RATTVSRLSASSVTPRVYPSYTSRPVGRTTAPLTTRRPPTTTASIDRPRTPGPQRPPK
                     SCDSQPCLHGGTCQDLDSGKGFSCSCTAGRAGTVCEKVQLPSVPAFKGHSFLAFPTLR
                     AYHTLRLALEFRALETEGLLLYNGNARGKDFLALALLDGHVQFRFDTGSGPAVLTSLV
                     PVEPGRWHRLELSRHWRQGTLSVDGEAPVVGESPSGTDGLNLDTKLYVGGLPEEQVAT
                     VLDRTSVGIGLKGCIRMLDINNQQLELSDWQRAVVQSSGVGECGDHPCSPNPCHGGAL
                     CQALEAGVFLCQCPPGRFGPTCADEKNPCQPNPCHGSAPCHVLSRGGAKCACPLGRSG
                     SFCETVLENAGSRPFLADFNGFSYLELKGLHTFERDLGEKMALEMVFLARGPSGLLLY
                     NGQKTDGKGDFVSLALHNRHLEFRYDLGKGAAIIRSKEPIALGTWVRVFLERNGRKGA
                     LQVGDGPRVLGESPVPHTMLNLKEPLYVGGAPDFSKLARGAAVASGFDGAIQLVSLRG
                     HQLLTQEHVLRAVDVAPFAGHPCTQAVDNPCLNGGSCIPREATYECLCPGGFSGLHCE
                     KGIVEKSVGDLETLAFDGRTYIEYLNAVTESEKALQSNHFELSLRTEATQGLVLWIGK
                     VGERADYMALAIVDGHLQLSYDLGSQPVVLRSTVKVNTNRWLRVRAHREHREGSLQVG
                     NEAPVTGSSPLGATQLDTDGALWLGGLQKLPVGQALPKAYGTGFVGCLRDVVVGHRQL
                     HLLEDAVTKPELRPCPTL"
     exon            276..537
                     /gene="Agrn"
                     /gene_synonym="Agrin; nmf380"
                     /inference="alignment:Splign:2.1.0"
     exon            538..585
                     /gene="Agrn"
                     /gene_synonym="Agrin; nmf380"
                     /inference="alignment:Splign:2.1.0"
     exon            586..801
                     /gene="Agrn"
                     /gene_synonym="Agrin; nmf380"
                     /inference="alignment:Splign:2.1.0"
     exon            802..1026
                     /gene="Agrn"
                     /gene_synonym="Agrin; nmf380"
                     /inference="alignment:Splign:2.1.0"
     exon            1027..1251
                     /gene="Agrn"
                     /gene_synonym="Agrin; nmf380"
                     /inference="alignment:Splign:2.1.0"
     exon            1252..1458
                     /gene="Agrn"
                     /gene_synonym="Agrin; nmf380"
                     /inference="alignment:Splign:2.1.0"
     exon            1459..1677
                     /gene="Agrn"
                     /gene_synonym="Agrin; nmf380"
                     /inference="alignment:Splign:2.1.0"
     exon            1678..1872
                     /gene="Agrn"
                     /gene_synonym="Agrin; nmf380"
                     /inference="alignment:Splign:2.1.0"
     exon            1873..2073
                     /gene="Agrn"
                     /gene_synonym="Agrin; nmf380"
                     /inference="alignment:Splign:2.1.0"
     exon            2074..2222
                     /gene="Agrn"
                     /gene_synonym="Agrin; nmf380"
                     /inference="alignment:Splign:2.1.0"
     exon            2223..2328
                     /gene="Agrn"
                     /gene_synonym="Agrin; nmf380"
                     /inference="alignment:Splign:2.1.0"
     exon            2329..2445
                     /gene="Agrn"
                     /gene_synonym="Agrin; nmf380"
                     /inference="alignment:Splign:2.1.0"
     exon            2446..2610
                     /gene="Agrn"
                     /gene_synonym="Agrin; nmf380"
                     /inference="alignment:Splign:2.1.0"
     exon            2611..2754
                     /gene="Agrn"
                     /gene_synonym="Agrin; nmf380"
                     /inference="alignment:Splign:2.1.0"
     exon            2755..2879
                     /gene="Agrn"
                     /gene_synonym="Agrin; nmf380"
                     /inference="alignment:Splign:2.1.0"
     exon            2880..2985
                     /gene="Agrn"
                     /gene_synonym="Agrin; nmf380"
                     /inference="alignment:Splign:2.1.0"
     exon            2986..3291
                     /gene="Agrn"
                     /gene_synonym="Agrin; nmf380"
                     /inference="alignment:Splign:2.1.0"
     exon            3292..3429
                     /gene="Agrn"
                     /gene_synonym="Agrin; nmf380"
                     /inference="alignment:Splign:2.1.0"
     exon            3430..3557
                     /gene="Agrn"
                     /gene_synonym="Agrin; nmf380"
                     /inference="alignment:Splign:2.1.0"
     exon            3558..3672
                     /gene="Agrn"
                     /gene_synonym="Agrin; nmf380"
                     /inference="alignment:Splign:2.1.0"
     exon            3673..3792
                     /gene="Agrn"
                     /gene_synonym="Agrin; nmf380"
                     /inference="alignment:Splign:2.1.0"
     exon            3793..4140
                     /gene="Agrn"
                     /gene_synonym="Agrin; nmf380"
                     /inference="alignment:Splign:2.1.0"
     exon            4141..4333
                     /gene="Agrn"
                     /gene_synonym="Agrin; nmf380"
                     /inference="alignment:Splign:2.1.0"
     exon            4334..4549
                     /gene="Agrn"
                     /gene_synonym="Agrin; nmf380"
                     /inference="alignment:Splign:2.1.0"
     exon            4550..4779
                     /gene="Agrn"
                     /gene_synonym="Agrin; nmf380"
                     /inference="alignment:Splign:2.1.0"
     exon            4780..4914
                     /gene="Agrn"
                     /gene_synonym="Agrin; nmf380"
                     /inference="alignment:Splign:2.1.0"
     exon            4915..5011
                     /gene="Agrn"
                     /gene_synonym="Agrin; nmf380"
                     /inference="alignment:Splign:2.1.0"
     exon            5012..5176
                     /gene="Agrn"
                     /gene_synonym="Agrin; nmf380"
                     /inference="alignment:Splign:2.1.0"
     exon            5177..5288
                     /gene="Agrn"
                     /gene_synonym="Agrin; nmf380"
                     /inference="alignment:Splign:2.1.0"
     exon            5289..5405
                     /gene="Agrn"
                     /gene_synonym="Agrin; nmf380"
                     /inference="alignment:Splign:2.1.0"
     exon            5406..5598
                     /gene="Agrn"
                     /gene_synonym="Agrin; nmf380"
                     /inference="alignment:Splign:2.1.0"
     exon            5599..5686
                     /gene="Agrn"
                     /gene_synonym="Agrin; nmf380"
                     /inference="alignment:Splign:2.1.0"
     exon            5687..5911
                     /gene="Agrn"
                     /gene_synonym="Agrin; nmf380"
                     /inference="alignment:Splign:2.1.0"
     exon            5912..6015
                     /gene="Agrn"
                     /gene_synonym="Agrin; nmf380"
                     /inference="alignment:Splign:2.1.0"
     exon            6016..7379
                     /gene="Agrn"
                     /gene_synonym="Agrin; nmf380"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7357..7362
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Agrn"
                     /gene_synonym="Agrin; nmf380"
                     /note="hexamer: AATAAA"
     polyA_site      7379
                     /gene="Agrn"
                     /gene_synonym="Agrin; nmf380"
                     /note="major polyA site"
ORIGIN      
        1 agtcctgccc cccgagcggt ccggcccgcg cgctctcccg gctccctccc gccgttccgg
       61 gctgcgccat ggtccgcccg cggctgagct tcccggcccc gctgctgccg ctgctgctgc
      121 tcctggcggc ggccgcgcca gccgtgccgg gcgccagcgg gacgtgcccg gagcgcgcct
      181 tggagcggcg cgaggaggag gcgaacgtgg tgctcaccgg cacagtggag gagatcctca
      241 acgtggaccc ggtgcagcac acctactcct gcaaggttcg agtgtggcgg tacttgaaag
      301 gcaaagatgt ggtggcccaa gagagcctgc tggatggtgg caacaaggtg gtaattggtg
      361 gctttggaga ccccctcatc tgcgacaacc aggtgtccac cggggacact agaatcttct
      421 ttgtgaaccc agcaccaccc tacctgtggc cagcccacaa gaatgagttg atgctcaact
      481 ccagccttat gcggatcact ttgcggaacc tggaagaggt ggagttctgt gtagaagaca
      541 aacctgggat ccacttcaca gcagcgcctt ctatgcctcc tgatgtgtgc aggggaatgt
      601 tatgtggctt tggtgctgtg tgtgaaccta gtgttgagga tccaggccgg gcctcctgtg
      661 tgtgcaagaa gaatgtctgc cctgctatgg tagctcctgt gtgtggctca gatgcttcca
      721 cctatagcaa cgagtgtgag ctacagcgtg cacagtgcaa ccagcaacgg cgcatccgcc
      781 tactccgcca agggccatgt gggtcccggg acccctgtgc caatgtgacc tgcagtttcg
      841 gtagtacctg tgtaccttca gccgatggac agaccgcctc gtgtctgtgt cctacaacct
      901 gctttggggc ccctgatggc acagtgtgtg gcagtgatgg tgttgactac cctagtgagt
      961 gccagctgct ccgtcatgcc tgtgccaacc aggagcacat ctttaagaag ttcgatggtc
     1021 cttgtgaccc ctgccagggc agcatgtcag acctgaatca tatttgccgg gtgaacccac
     1081 gtacacggca cccagaaatg cttctgcggc ctgagaactg ccccgcccaa cacacaccta
     1141 tctgtggaga tgatggggtc acctatgaaa acgactgtgt catgagccgt ataggtgcag
     1201 cccgtggcct gcttctccag aaagtgcgct ctggtcaatg ccagactcga gaccagtgcc
     1261 cggagacctg ccagtttaac tctgtatgcc tgtcccgccg cggccgtccc cactgttcct
     1321 gcgatcgcgt cacctgtgat ggggcttaca ggccagtgtg tgcccaggat gggcacacgt
     1381 atgacaatga ctgttggcgc caacaggccg agtgtcgaca acagcagacc attcccccca
     1441 agcaccaggg cccgtgtgac cagaccccat ccccgtgccg tggagcgcag tgtgcatttg
     1501 gggcaacatg cacagtgaag aatgggaaag ctgtgtgcga gtgccagcgg gtgtgctcgg
     1561 gcggctacga tcctgtgtgc ggcagtgatg gtgtcactta cggcagtgtg tgcgagctgg
     1621 aatccatggc ctgtaccctt gggcgggaaa tccgagtggc ccgcagagga ccgtgtgacc
     1681 gatgtgggca gtgccggttt ggatccttgt gcgaggtgga gactggacgc tgtgtgtgcc
     1741 cctctgagtg tgtggagtca gcccagcccg tatgtggctc tgacggacac acatatgcta
     1801 gtgaatgtga gctgcatgtc cacgcctgta cacaccagat cagcctatac gtggcctcag
     1861 ccggacactg ccagacctgt ggagaaacag tttgtacctt tggggctgtg tgctcagctg
     1921 gacagtgtgt atgtccccgt tgtgagcacc ccccacctgg ccctgtgtgc ggcagtgatg
     1981 gcgtcaccta cctcagtgcc tgtgagctcc gagaggctgc ctgtcagcag caggtacaaa
     2041 ttgaggaggc ccgtgcaggg ccgtgtgagc cggctgagtg tggctcaggg ggctctgggt
     2101 ctggggaaga caatgcgtgt gagcaggagc tgtgtcggca gcatggtggt gtctgggatg
     2161 aggactcaga agacgggccg tgtgtctgtg actttagttg ccagagtgtc cttaaaagcc
     2221 cagtgtgtgg ctcagatgga gtcacctata gcacggagtg ccatctgaag aaggccagat
     2281 gtgaagcgcg gcaagagctg tacgtcgctg ctcagggagc ctgccggggc cctaccttgg
     2341 ctccactgct acctatggcc tccccacact gtgcccaaac cccctatggc tgctgccagg
     2401 acaatgtcac tgctgcccag ggtgtgggct tggctggctg tcccagcacc tgccattgca
     2461 acccacacgg ctcctatagc ggcacttgtg acccagtcac agggcagtgc tcctgccgac
     2521 caggtgtagg aggcctcagg tgtgatcgct gtgagcctgg cttctggaac ttccgtggca
     2581 ttgtcaccga tggacatagt ggttgcactc cctgcagctg tgaccctcgg ggtgctgtaa
     2641 gagatgactg tgagcagatg actggattgt gttcctgtag acctggtgtg gctggtccca
     2701 agtgtgggca gtgtccagat ggtcaagccc tgggccatct tggctgtgaa gcagatccca
     2761 caacaccagt gacttgtgtg gaaatgcact gtgagtttgg cgcctcctgc gtagaggagg
     2821 ctggttttgc ccagtgtgtc tgcccaactc tcacatgtcc agaggctaac tctaccaagg
     2881 tctgtggatc agatggtgtc acatacggca atgaatgcca gctgaagacc attgcctgcc
     2941 gccagcgtct ggacatctcc attcagagtc ttggtccatg ccgggagagt gttgctcctg
     3001 gggtttcccc tacatctgca tctatgacca ccccaaggca tatcctgagc aggacactgg
     3061 cgtctcccca cagcagcctt cctctgtctc ccagcactac tgcccatgat tggcccaccc
     3121 cattacccac atcacctcag accgtagtcg gcacccccag gagcactgca gccacaccct
     3181 ctgatgtggc cagtcttgct acagcgatct tcagggaatc tggcagcacc aacggcagtg
     3241 gcgatgagga gctcagtggc gatgaggagg ccagtggggg cgggtctggg ggacttgagc
     3301 ccccggtggg cagcgttgtg gtgacccacg ggccacccat cgagagggct tcctgttaca
     3361 actcaccttt gggctgctgc tcagatggca agacaccctc actggactca gaaggctcca
     3421 actgtccagc taccaaggca ttccagggcg tgctggagct tgagggggtc gagggacagg
     3481 aactgttcta cacaccagag atggctgacc ccaagtcaga gttgtttggg gagactgcaa
     3541 ggagcattga gagcacgctg gacgacctgt tccggaattc ggatgttaag aaggacttct
     3601 ggagcatccg cctacgggaa ctggggcctg gcaaattagt ccgtgccatt gtggatgttc
     3661 actttgaccc caccacagcc ttccaggcac cagatgtggg tcaggccttg ctccaacaga
     3721 tccaggtatc caggccgtgg gccctggcag tgaggaggcc tctgcgggag catgtgcgat
     3781 tcttggactt tgactggttt cccacttttt ttacgggagc tgcaacagga accacagctg
     3841 ctgtggccac agccagagcc accactgtga gccgactgtc tgcctcttct gtcaccccac
     3901 gagtctaccc cagttacacc agccggcctg ttggcagaac tacggcaccg ctaaccactc
     3961 gccggccacc aaccactacc gccagtattg accgacctcg gactccaggc ccgcaacggc
     4021 ccccaaagtc ctgtgattcc cagccttgcc tccacggagg tacctgccag gacctggatt
     4081 ctggcaaggg tttcagctgc agctgtactg caggcagggc tggcactgtc tgtgagaaag
     4141 tgcagctccc ctctgtgcca gcttttaagg gccactcctt cttggccttc cccaccctcc
     4201 gagcctacca cacgctgcgt ctggcactag aattccgggc gctggagaca gagggactgc
     4261 tgctctacaa tggcaatgca cgtggcaaag atttcctggc tctggctctg ttggatggtc
     4321 atgtacagtt caggttcgac acgggctcag ggccggcggt gctaacaagc ttagtgccag
     4381 tggaaccggg acggtggcac cgcctcgagt tgtcacggca ttggcggcag ggcacacttt
     4441 ctgtggatgg cgaggctcct gttgtaggtg aaagtccgag tggcactgat ggcctcaact
     4501 tggacacgaa gctctatgtg ggtggtctcc cagaagaaca agttgccacg gtgcttgatc
     4561 ggacctctgt gggcatcggc ctgaaaggat gcattcgtat gttggacatc aacaaccagc
     4621 agctggagct gagcgattgg cagagggctg tggttcaaag ctctggtgtg ggggaatgcg
     4681 gagaccatcc ctgctcacct aacccctgcc atggcggggc cctctgccag gccctggagg
     4741 ctggcgtgtt cctctgtcag tgcccacctg gccgctttgg cccaacttgt gcagatgaaa
     4801 agaacccctg ccaaccgaac ccctgccacg ggtcagcccc ctgccatgtg ctttccaggg
     4861 gtggggccaa gtgtgcgtgc cccctgggac gcagtggttc cttctgtgag acagtcctgg
     4921 agaatgctgg ctcccggccc ttcctggctg actttaatgg cttctcctac ctggaactga
     4981 aaggcttgca caccttcgag agagacctag gggagaagat ggcgctggag atggtgttct
     5041 tggctcgtgg gcccagtggc ttactcctct acaatgggca gaagacggat ggcaaggggg
     5101 actttgtatc cctggccctg cataaccggc acctagagtt ccgctatgac cttggcaagg
     5161 gggctgcaat catcaggagc aaagagccca tagccctggg cacctgggtt agggtattcc
     5221 tggaacgaaa tggccgcaag ggtgcccttc aagtgggtga tgggccccgt gtgctagggg
     5281 aatctccggt cccgcacacc atgctcaacc tcaaggagcc cctctatgtg gggggagctc
     5341 ctgacttcag caagctggct cggggcgctg cagtggcctc cggctttgat ggtgccatcc
     5401 agctggtgtc tctaagaggc catcagctgc tgactcagga gcatgtgttg cgggcagtag
     5461 atgtagcgcc ttttgcaggc cacccttgta cccaggccgt ggacaacccc tgccttaatg
     5521 ggggctcctg tatcccgagg gaagccactt atgagtgcct gtgtcctggg ggcttctctg
     5581 ggctgcactg cgagaagggg atagttgaga agtcagtggg ggacctagaa acactggcct
     5641 ttgatgggcg gacctacatc gagtacctca atgctgtgac tgagagtgag aaagcgctgc
     5701 agagcaacca ctttgagctg agcttacgca ctgaggccac gcaggggctg gtgctgtgga
     5761 ttggaaaggt tggagaacgt gcagactaca tggctctggc cattgtggat gggcacctac
     5821 aactgagcta tgacctaggc tcccagccag ttgtgctgcg ctccactgtg aaggtcaaca
     5881 ccaaccgctg gcttcgagtc agggctcaca gggagcacag ggaaggttcc cttcaggtgg
     5941 gcaatgaagc ccctgtgact ggctcttccc cgctgggtgc cacacaattg gacacagatg
     6001 gagccctgtg gcttggaggc ctacagaagc ttcctgtggg gcaggctctc cccaaggcct
     6061 atggcacggg ttttgtgggc tgtctgcggg acgtggtagt gggccatcgc cagctgcatc
     6121 tgctggagga cgctgtcacc aaaccagagc taagaccctg ccccactctc tgagctgcca
     6181 ctagagctgc caccttccct cctgtaatta ttttctattt ttgtaaactt gttgcatttt
     6241 tatatgattt tcttgactgc acgttggcca gaaggactgc tggcctggcc tcctttccgt
     6301 ccgggcagct gtgctgcaga gacaaaaagt ggtgctgagg gactcctaag tcggccgtag
     6361 cacctcgagc cctgggcttt gaaggcagcc ttaggacaaa ctcctctgcc taaggcatca
     6421 tgcctcagcc ccttgcactg tatgttccta tatgtctact tgtattgatc ctttgggtct
     6481 cctggcccat tgctaggcac tgtttgtgtg ttattaatgt gtaattgggt gagaactgtg
     6541 tccctgagca ggaggggctg ttggggttga gggggccttc attaaaccaa gtaacattgg
     6601 gtccttttta ggtcccactt aagctgctct cctgtgtgtg ctgagggcca gcctcacccc
     6661 aggctagcct ggacccccag tgctccctgt gacaatactg tgatatcaaa aaactgataa
     6721 taattgggac atcatcttac ttcccaaaat gtgtttgcct gtccccggag gctggaagta
     6781 gaagccaggc tacatctaag ttgccctgac tggccttcag ctggccttac ccacttcttc
     6841 agttaccctg aacatgggga gtgcatcacg atagggtgtg gctgacctcc attgcttacc
     6901 tgcctgagta gccagctgca tccatctctc aaagatacct agccaggctc ctaaccatgt
     6961 tcttcaacca ttgattttgt ttgatttctg atgtggaagt gcggtcattc ctggtttaac
     7021 tcacagcaac ttttaattgg tctaagatga gggcatccct cagttggtcc attgtaatga
     7081 tgatcgtcct catctgagtg acagttactg cccagggtcc agccaaggac aggtacagga
     7141 aggcagagat cactatagag ccaaaaatga ctgaaactga agcatccatc tgcttctcca
     7201 ttccctgtct tttaagtcca gcacattggg agacaaacta agcaaatctg ggatgggggc
     7261 agtctgacct tcttcctgct tcggtgatcc gaggctagag ggagctgcat ggtatcttgt
     7321 tactaactca gtatgtgtca atgtcaaata ccatgaaata aagtctgaaa accttaaaa
//
