LOCUS       NM_001414237            2498 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus 1-acylglycerol-3-phosphate O-acyltransferase 1
            (Agpat1), transcript variant 4, mRNA.
ACCESSION   NM_001414237 XM_006524648
VERSION     NM_001414237.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Agarwal,A.K., Tunison,K., Dalal,J.S., Nagamma,S.S., Hamra,F.K.,
            Sankella,S., Shao,X., Auchus,R.J. and Garg,A.
  TITLE     Metabolic, Reproductive, and Neurologic Abnormalities in
            Agpat1-Null Mice
  JOURNAL   Endocrinology 158 (11), 3954-3973 (2017)
   PUBMED   28973305
  REMARK    GeneRIF: Agpat1-/- mice developed widespread disturbances of
            metabolism, sperm development, and neurologic function resulting
            from disrupted phospholipid homeostasis. AGPAT1 appears to serve
            important functions in the physiology of multiple organ systems.
            The Agpat1-deficient mouse provides an important model in which to
            study the contribution of phospholipid and triacylglycerol
            synthesis to physiology and diseases.
REFERENCE   3  (bases 1 to 2498)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   4  (bases 1 to 2498)
  AUTHORS   Subauste,A.R., Elliott,B., Das,A.K. and Burant,C.F.
  TITLE     A role for 1-acylglycerol-3-phosphate-O-acyltransferase-1 in
            myoblast differentiation
  JOURNAL   Differentiation 80 (2-3), 140-146 (2010)
   PUBMED   20561744
  REMARK    GeneRIF: observations strongly implicate AGPAT1 in the development
            of skeletal muscle, specifically to terminal differentiation. These
            findings are linked to the regulation of actin cytoskeleton
REFERENCE   5  (bases 1 to 2498)
  AUTHORS   Tamplin,O.J., Kinzel,D., Cox,B.J., Bell,C.E., Rossant,J. and
            Lickert,H.
  TITLE     Microarray analysis of Foxa2 mutant mouse embryos reveals novel
            gene expression and inductive roles for the gastrula organizer and
            its derivatives
  JOURNAL   BMC Genomics 9, 511 (2008)
   PUBMED   18973680
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2498)
  AUTHORS   Lu,B., Jiang,Y.J., Zhou,Y., Xu,F.Y., Hatch,G.M. and Choy,P.C.
  TITLE     Cloning and characterization of murine 1-acyl-sn-glycerol
            3-phosphate acyltransferases and their regulation by PPARalpha in
            murine heart
  JOURNAL   Biochem J 385 (Pt 2), 469-477 (2005)
   PUBMED   15367102
REFERENCE   7  (bases 1 to 2498)
  AUTHORS   Xie,T., Rowen,L., Aguado,B., Ahearn,M.E., Madan,A., Qin,S.,
            Campbell,R.D. and Hood,L.
  TITLE     Analysis of the gene-dense major histocompatibility complex class
            III region and its comparison to mouse
  JOURNAL   Genome Res 13 (12), 2621-2636 (2003)
   PUBMED   14656967
REFERENCE   8  (bases 1 to 2498)
  AUTHORS   Allcock,R.J., Martin,A.M. and Price,P.
  TITLE     The mouse as a model for the effects of MHC genes on human disease
  JOURNAL   Immunol Today 21 (7), 328-332 (2000)
   PUBMED   10871872
  REMARK    Review article
REFERENCE   9  (bases 1 to 2498)
  AUTHORS   Araki,K., Imaizumi,T., Sekimoto,T., Yoshinobu,K., Yoshimuta,J.,
            Akizuki,M., Miura,K., Araki,M. and Yamamura,K.
  TITLE     Exchangeable gene trap using the Cre/mutated lox system
  JOURNAL   Cell Mol Biol (Noisy-le-grand) 45 (5), 737-750 (1999)
   PUBMED   10512203
REFERENCE   10 (bases 1 to 2498)
  AUTHORS   Kume,K. and Shimizu,T.
  TITLE     cDNA cloning and expression of murine
            1-acyl-sn-glycerol-3-phosphate acyltransferase
  JOURNAL   Biochem Biophys Res Commun 237 (3), 663-666 (1997)
   PUBMED   9299423
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CT009767.6.
            
            On Nov 16, 2022 this sequence version replaced XM_006524648.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR7345562.989285.1,
                                           SRR5189685.152774.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-650               CT009767.6         161720-162369
            651-850             CT009767.6         167872-168071
            851-984             CT009767.6         168299-168432
            985-1160            CT009767.6         168701-168876
            1161-1256           CT009767.6         169032-169127
            1257-1332           CT009767.6         169218-169293
            1333-2498           CT009767.6         169680-170845
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="17"
                     /map="17 18.18 cM"
     gene            1..2498
                     /gene="Agpat1"
                     /gene_synonym="1-AGP; 1-AGPAT"
                     /note="1-acylglycerol-3-phosphate O-acyltransferase 1"
                     /db_xref="GeneID:55979"
                     /db_xref="MGI:MGI:1932075"
     exon            1..650
                     /gene="Agpat1"
                     /gene_synonym="1-AGP; 1-AGPAT"
                     /inference="alignment:Splign:2.1.0"
     exon            651..850
                     /gene="Agpat1"
                     /gene_synonym="1-AGP; 1-AGPAT"
                     /inference="alignment:Splign:2.1.0"
     CDS             660..1517
                     /gene="Agpat1"
                     /gene_synonym="1-AGP; 1-AGPAT"
                     /EC_number="2.3.1.51"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 4; 1-acyl-sn-glycerol-3-phosphate acyltransferase
                     alpha; 1-AGPAT 1; 1-AGP acyltransferase 1;
                     lysophosphatidic acid acyltransferase alpha; Lpaat-alpha;
                     1-acylglycerol-3-phosphate O-acyltransferase 1
                     (lysophosphatidic acid acyltransferase, alpha)"
                     /codon_start=1
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase
                     alpha isoform 1 precursor"
                     /protein_id="NP_001401166.1"
                     /db_xref="GeneID:55979"
                     /db_xref="MGI:MGI:1932075"
                     /translation="MELWPGAWTALLLLLLLLLSTLWFCSSSAKYFFKMAFYNGWILF
                     LAILAIPVCAVRGRNVENMKILRLLLLHVKYLYGIRVEVRGAHHFPPTQPYVVVSNHQ
                     SSLDLLGMMEVLPDRCVPIAKRELLWAGSAGLACWLAGIIFIDRKRTGDAISVMSEVA
                     QTLLTQDVRVWVFPEGTRNHNGSMLPFKRGAFHLAVQAQVPIIPIVMSSYQDFYSKKE
                     RRFTSPGRCQVRVLPPVSTEGLTPDDVPALADSVRHSMLTIFREISTDGLGGGDCLKK
                     PGGAGEARL"
     exon            851..984
                     /gene="Agpat1"
                     /gene_synonym="1-AGP; 1-AGPAT"
                     /inference="alignment:Splign:2.1.0"
     exon            985..1160
                     /gene="Agpat1"
                     /gene_synonym="1-AGP; 1-AGPAT"
                     /inference="alignment:Splign:2.1.0"
     exon            1161..1256
                     /gene="Agpat1"
                     /gene_synonym="1-AGP; 1-AGPAT"
                     /inference="alignment:Splign:2.1.0"
     exon            1257..1332
                     /gene="Agpat1"
                     /gene_synonym="1-AGP; 1-AGPAT"
                     /inference="alignment:Splign:2.1.0"
     exon            1333..2498
                     /gene="Agpat1"
                     /gene_synonym="1-AGP; 1-AGPAT"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      1803
                     /gene="Agpat1"
                     /gene_synonym="1-AGP; 1-AGPAT"
     regulatory      2481..2486
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Agpat1"
                     /gene_synonym="1-AGP; 1-AGPAT"
                     /note="hexamer: AATAAA"
     polyA_site      2498
                     /gene="Agpat1"
                     /gene_synonym="1-AGP; 1-AGPAT"
                     /note="major polyA site"
ORIGIN      
        1 agaagtacca gggtcgcagg gttcaagatg gctccggcct ccttgggtga cgtagaggga
       61 agaccttggg tccgcccctc cctctcggag aagcggggag cagagctggg gttagcccga
      121 cgcgtggatg gtgcggccgt cggtctgtgg cgccggtgtt aaccccactc tctcggttgg
      181 acgacttagc ctctctaggt gattctcaca gcaggaccga gccaaaggcc caccgttgta
      241 atgtgacaca gcagaccaca gtggaccccc actccgtcct aaaccttaca agcagaaaag
      301 gactggttcc accctcttgg gagtgggcgc ccaaaggaca gaggccgacc cctaggtgac
      361 ccgccgtaga atggggtaga tccgcccctt ccttgtagtt tgccctgcat agatttggat
      421 ctttttcctg gctggacggc tggcagctag ggcctttcca gcactcagtg accctcacta
      481 tggcccaagg cccagaatta ggtaacagtc ctcgcattcg caagggctct tcaccaagcc
      541 atgtaaatca cccagggcaa aggtgtccat gggcgggaga cgagaacacg ctagctaagg
      601 attattcaat accttgataa aggacctgtg tgactgagga ataaagaaag gtgaccagaa
      661 tggagctgtg gcccggggcc tggacggcgc tgctgctgct gctgctcctg ctgctgtcca
      721 ccctgtggtt ctgcagctcc agtgccaagt atttctttaa gatggccttc tacaacggct
      781 ggatcctctt cctggcgatc ctcgcaatcc ccgtgtgtgc tgtgcgaggg cgcaatgtcg
      841 agaacatgaa gatcttgcgt ctgctgctgc tccacgtcaa atacctgtat gggatccgag
      901 tggaggtgcg cggggctcac cacttccctc ccacgcagcc ctacgtggtt gtgtccaacc
      961 accagagttc cctcgacctg cttggaatga tggaggtcct gccagatcgt tgtgtgccca
     1021 ttgccaagcg tgagctacta tgggctggct ctgctgggct ggcctgctgg ctggcaggaa
     1081 tcatcttcat tgaccggaaa cgcacagggg acgccatcag tgtcatgtcc gaggtggctc
     1141 agaccttgct cacccaggat gtgagagtct gggtttttcc tgaaggaaca agaaaccaca
     1201 atggctccat gctgcccttc aaacgaggcg ccttccacct tgcagtacag gcccaggtcc
     1261 ccatcatccc catcgtcatg tcctcctatc aagacttcta ctccaaaaag gaaagacgct
     1321 tcacctcgcc tggacgttgt caggtgcggg tgctgcctcc agtgtccaca gaagggctga
     1381 caccagatga cgtcccagct ctggcagaca gcgtccggca ctccatgctc accatcttcc
     1441 gggagatctc cactgacggc ctgggtggtg gcgactgtct gaagaagcct gggggagcgg
     1501 gcgaagcccg gctctgagct ccaccccctt cttcctccct gtacctccca cccagggtct
     1561 gaggcagggc caggccctct tcccgggtcc ccactccttc ctctcctttt ggaatcctca
     1621 acttctgaag tgaatgtcac tccatgttct ttccctgccc cacccttgga ctctcctgcg
     1681 tgggtgcagt ctccactcca agccccacct gccatcttgt cttgtgggac agttgcctcc
     1741 cctcatctca agtggctcat ccgatacaag ggtggaagac attccatccc acccgtccct
     1801 caatggtctt tgtgtggcct tctctccctc tccgccctac actggacaat ggacttgtct
     1861 gttgcgttct gaggagcaat ctatccttca cccccaagtc cacagcctga tggggctcgt
     1921 ctcctagtga ggactcctca tcctgtggct ggaagctgca cctgacactc ctcacaggct
     1981 cacctgggga ctgactgcct cagtactgtc acctccatgg ggctgacacc caccatgggg
     2041 ctggctcgtg taactcagag atccctgccc gtgccagggc tgagcatact ccagatgcca
     2101 atttctgctt ccccctagcc tcacccctgc cccaggtagt ggtcttcagg gtcctggccg
     2161 ggcaccatct aaccaaattc tggagaggca gacgccagaa gctgctctct gctccctggg
     2221 gccttaggga gaagaccctg accctggaga gaaggagagg agggcgctta atttatttct
     2281 tttttttttt tttttttttt tttgaggtac ctccgtctct gagccaattt tcatttcctc
     2341 ccagtggcgt tggccacccc tgcctcccac tctagaccca ttcctaaaac ccaggaggta
     2401 gactgggggg aaggaggaac gggtggggta cagttttgtg tggttggttt ttattatctg
     2461 gataacagca agcaactgaa aataaagaga aagagaga
//
