LOCUS       NM_153178              13793 bp    mRNA    linear   ROD 04-MAR-2025
DEFINITION  Mus musculus argonaute RISC catalytic subunit 2 (Ago2), mRNA.
ACCESSION   NM_153178
VERSION     NM_153178.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 13793)
  AUTHORS   Quiroga,D., Roman,B., Salih,M., Daccarett-Bojanini,W.N., Garbus,H.,
            Ebenebe,O.V., Dodd-O,J.M., O'Rourke,B., Kohr,M. and Das,S.
  TITLE     Sex-dependent phosphorylation of Argonaute 2 reduces the
            mitochondrial translocation of miR-181c and induces
            cardioprotection in females
  JOURNAL   J Mol Cell Cardiol 194, 59-69 (2024)
   PUBMED   38880194
  REMARK    GeneRIF: Sex-dependent phosphorylation of Argonaute 2 reduces the
            mitochondrial translocation of miR-181c and induces
            cardioprotection in females.
REFERENCE   2  (bases 1 to 13793)
  AUTHORS   Huang,Y., Yin,G.N., Liu,F.Y., Fridayana,F.R., Niloofar,L., Vo,M.N.
            and Ryu,J.K.
  TITLE     Argonaute 2 restored erectile function and corpus cavernosum
            mitochondrial function by reducing apoptosis in a mouse model of
            cavernous nerve injury
  JOURNAL   Investig Clin Urol 65 (4), 400-410 (2024)
   PUBMED   38978220
  REMARK    GeneRIF: Argonaute 2 restored erectile function and corpus
            cavernosum mitochondrial function by reducing apoptosis in a mouse
            model of cavernous nerve injury.
REFERENCE   3  (bases 1 to 13793)
  AUTHORS   Li,J., Yao,H., Zhao,F., An,J., Wang,Q., Mu,J., Liu,Z., Zou,M.H. and
            Xie,Z.
  TITLE     Pycard deficiency inhibits microRNA maturation and prevents
            neointima formation by promoting chaperone-mediated autophagic
            degradation of AGO2/argonaute 2 in adipose tissue
  JOURNAL   Autophagy 20 (3), 629-644 (2024)
   PUBMED   37963060
  REMARK    GeneRIF: Pycard deficiency inhibits microRNA maturation and
            prevents neointima formation by promoting chaperone-mediated
            autophagic degradation of AGO2/argonaute 2 in adipose tissue.
REFERENCE   4  (bases 1 to 13793)
  AUTHORS   Sala,L., Kumar,M., Prajapat,M., Chandrasekhar,S., Cosby,R.L., La
            Rocca,G., Macfarlan,T.S., Awasthi,P., Chari,R., Kruhlak,M. and
            Vidigal,J.A.
  TITLE     AGO2 silences mobile transposons in the nucleus of quiescent cells
  JOURNAL   Nat Struct Mol Biol 30 (12), 1985-1995 (2023)
   PUBMED   37985687
  REMARK    GeneRIF: AGO2 silences mobile transposons in the nucleus of
            quiescent cells.
REFERENCE   5  (bases 1 to 13793)
  AUTHORS   Feng,Z. and Wu,J.
  TITLE     hsa_circ_0129047 Upregulates LYVE1 to Inhibit Hepatocellular
            Carcinoma Progression by Sponging miR-492
  JOURNAL   Dis Markers 2023, 6978234 (2023)
   PUBMED   37810197
  REMARK    GeneRIF: hsa_circ_0129047 Upregulates LYVE1 to Inhibit
            Hepatocellular Carcinoma Progression by Sponging miR-492.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 13793)
  AUTHORS   Lu,J., Qian,J., Chen,F., Tang,X., Li,C. and Cardoso,W.V.
  TITLE     Differential expression of components of the microRNA machinery
            during mouse organogenesis
  JOURNAL   Biochem Biophys Res Commun 334 (2), 319-323 (2005)
   PUBMED   16036130
REFERENCE   7  (bases 1 to 13793)
  AUTHORS   McKee,A.E., Minet,E., Stern,C., Riahi,S., Stiles,C.D. and
            Silver,P.A.
  TITLE     A genome-wide in situ hybridization map of RNA-binding proteins
            reveals anatomically restricted expression in the developing mouse
            brain
  JOURNAL   BMC Dev Biol 5, 14 (2005)
   PUBMED   16033648
  REMARK    Publication Status: Online-Only
REFERENCE   8  (bases 1 to 13793)
  AUTHORS   Liu,J., Carmell,M.A., Rivas,F.V., Marsden,C.G., Thomson,J.M.,
            Song,J.J., Hammond,S.M., Joshua-Tor,L. and Hannon,G.J.
  TITLE     Argonaute2 is the catalytic engine of mammalian RNAi
  JOURNAL   Science 305 (5689), 1437-1441 (2004)
   PUBMED   15284456
  REMARK    GeneRIF: essential for mouse development and cells lacking
            Argonaute2 are unable to mount an experimental response to siRNAs
REFERENCE   9  (bases 1 to 13793)
  AUTHORS   Doi,N., Zenno,S., Ueda,R., Ohki-Hamazaki,H., Ui-Tei,K. and Saigo,K.
  TITLE     Short-interfering-RNA-mediated gene silencing in mammalian cells
            requires Dicer and eIF2C translation initiation factors
  JOURNAL   Curr Biol 13 (1), 41-46 (2003)
   PUBMED   12526743
REFERENCE   10 (bases 1 to 13793)
  AUTHORS   Carmell,M.A., Xuan,Z., Zhang,M.Q. and Hannon,G.J.
  TITLE     The Argonaute family: tentacles that reach into RNAi, developmental
            control, stem cell maintenance, and tumorigenesis
  JOURNAL   Genes Dev 16 (21), 2733-2742 (2002)
   PUBMED   12414724
  REMARK    Review article
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC161581.3 and AC118640.8.
            
            On Jul 1, 2022 this sequence version replaced NM_153178.4.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC096465.1, SRR13948564.4563749.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-168               AC161581.3         105098-105265       c
            169-364             AC118640.8         34597-34792
            365-485             AC118640.8         43049-43169
            486-667             AC118640.8         50120-50301
            668-804             AC118640.8         52702-52838
            805-939             AC118640.8         54074-54208
            940-1027            AC118640.8         54628-54715
            1028-1175           AC118640.8         55851-55998
            1176-1295           AC118640.8         56913-57032
            1296-1418           AC118640.8         57163-57285
            1419-1552           AC118640.8         57706-57839
            1553-1737           AC118640.8         59914-60098
            1738-1897           AC118640.8         61666-61825
            1898-1988           AC118640.8         64769-64859
            1989-2183           AC118640.8         67260-67454
            2184-2318           AC118640.8         69341-69475
            2319-2420           AC118640.8         72689-72790
            2421-2620           AC118640.8         73798-73997
            2621-13793          AC118640.8         74146-85318
FEATURES             Location/Qualifiers
     source          1..13793
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="15"
                     /map="15 33.92 cM"
     gene            1..13793
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
                     /note="argonaute RISC catalytic subunit 2"
                     /db_xref="GeneID:239528"
                     /db_xref="MGI:MGI:2446632"
     exon            1..168
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
                     /inference="alignment:Splign:2.1.0"
     CDS             147..2729
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
                     /EC_number="3.1.26.n2"
                     /note="argonaute 2; Piwi/Argonaute family protein meIF2C2;
                     eukaryotic translation initiation factor 2C, 2; argonaute
                     RISC catalytic component 2"
                     /codon_start=1
                     /product="protein argonaute-2"
                     /protein_id="NP_694818.3"
                     /db_xref="CCDS:CCDS37098.1"
                     /db_xref="GeneID:239528"
                     /db_xref="MGI:MGI:2446632"
                     /translation="MYSGAGPVLASPAPTTSPIPGYAFKPPPRPDFGTTGRTIKLQAN
                     FFEMDIPKIDIYHYELDIKPEKCPRRVNREIVEHMVQHFKTQIFGDRKPVFDGRKNLY
                     TAMPLPIGRDKVELEVTLPGEGKDRIFKVSIKWVSCVSLQALHDALSGRLPSVPFETI
                     QALDVVMRHLPSMRYTPVGRSFFTASEGCSNPLGGGREVWFGFHQSVRPSLWKMMLNI
                     DVSATAFYKAQPVIEFVCEVLDFKSIEEQQKPLTDSQRVKFTKEIKGLKVEITHCGQM
                     KRKYRVCNVTRRPASHQTFPLQQESGQTVECTVAQYFKDRHKLVLRYPHLPCLQVGQE
                     QKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIRATARSAPDRQEEISKLMRSASFNTD
                     PYVREFGIMVKDEMTDVTGRVLQPPSILYGGRNKAIATPVQGVWDMRNKQFHTGIEIK
                     VWAIACFAPQRQCTEVHLKSFTEQLRKISRDAGMPIQGQPCFCKYAQGADSVEPMFRH
                     LKNTYAGLQLVVVILPGKTPVYAEVKRVGDTVLGMATQCVQMKNVQRTTPQTLSNLCL
                     KINVKLGGVNNILLPQGRPPVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPN
                     RYCATVRVQQHRQEIIQDLAAMVRELLIQFYKSTRFKPTRIIFYRDGVSEGQFQQVLH
                     HELLAIREACIKLEKDYQPGITFIVVQKRHHTRLFCTDKNERVGKSGNIPAGTTVDTK
                     ITHPTEFDFYLCSHAGIQGTSRPSHYHVLWDDNRFSSDELQILTYQLCHTYVRCTRSV
                     SIPAPAYYAHLVAFRARYHLVDKEHDSAEGSHTSGQSNGRDHQALAKAVQVHQDTLRT
                     MYFA"
     exon            169..364
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
                     /inference="alignment:Splign:2.1.0"
     exon            365..485
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
                     /inference="alignment:Splign:2.1.0"
     exon            486..667
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
                     /inference="alignment:Splign:2.1.0"
     exon            668..804
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
                     /inference="alignment:Splign:2.1.0"
     exon            805..939
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
                     /inference="alignment:Splign:2.1.0"
     exon            940..1027
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
                     /inference="alignment:Splign:2.1.0"
     exon            1028..1175
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
                     /inference="alignment:Splign:2.1.0"
     exon            1176..1295
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
                     /inference="alignment:Splign:2.1.0"
     exon            1296..1418
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
                     /inference="alignment:Splign:2.1.0"
     exon            1419..1552
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
                     /inference="alignment:Splign:2.1.0"
     exon            1553..1737
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
                     /inference="alignment:Splign:2.1.0"
     exon            1738..1897
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
                     /inference="alignment:Splign:2.1.0"
     exon            1898..1988
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
                     /inference="alignment:Splign:2.1.0"
     exon            1989..2183
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
                     /inference="alignment:Splign:2.1.0"
     exon            2184..2318
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
                     /inference="alignment:Splign:2.1.0"
     exon            2319..2420
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
                     /inference="alignment:Splign:2.1.0"
     exon            2421..2620
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
                     /inference="alignment:Splign:2.1.0"
     exon            2621..13793
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
                     /inference="alignment:Splign:2.1.0"
     regulatory      11023..11028
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
                     /note="hexamer: ATTATA"
     polyA_site      11045
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
                     /note="major polyA site"
     regulatory      13764..13769
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
                     /note="hexamer: AATAAA"
     polyA_site      13793
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
ORIGIN      
        1 gccgccccgg ccgcgtcggg taaacctgtt ctgcgaggcg gccgcgccgg ggcggatcga
       61 gcggccggcg gctcctcgcg gctcgcggcg tcggggcccg tggagcgcgc ggccggccct
      121 cggccgcggc cctcggcaac gccaccatgt actcgggagc cggccccgtt cttgcttctc
      181 ctgctccgac aacatcaccc atcccaggat atgccttcaa acctccacct cggccggact
      241 tcggcaccac cgggagaaca atcaaactac aggccaattt ctttgaaatg gacatcccca
      301 aaattgacat ctatcactat gaattggaca tcaaacctga gaaatgccct cggagagtga
      361 acagggaaat tgtggagcac atggtccagc actttaaaac tcagatcttc ggggaccgga
      421 agccagtgtt tgatggaagg aagaatctgt acacagcaat gccccttccg atcggcaggg
      481 acaaggtgga gctggaggtc acgctcccgg gagaaggcaa agatcgcatc tttaaggtat
      541 ccatcaagtg ggtgtcgtgc gtgagcctgc aggcgttaca cgatgcactt tcggggcggc
      601 tgcccagcgt ccccttcgag acgatccagg ccctggacgt tgtcatgagg cacttaccat
      661 ccatgaggta cacccctgtt ggccgttcct tcttcactgc atctgaaggc tgttccaacc
      721 ctctgggtgg gggcagagaa gtgtggtttg gcttccatca gtccgtccga ccttctcttt
      781 ggaaaatgat gctgaatatt gatgtatcgg caacagcgtt ttacaaggca cagccagtga
      841 tcgagtttgt ttgtgaagtt ttggatttta aaagtattga agaacaacaa aaacctctga
      901 cagattccca aagggtaaag tttaccaaag aaatcaaagg tctaaaggtg gagataacgc
      961 actgtggtca gatgaagagg aagtaccgtg tctgcaatgt gacccggcgg cctgccagtc
     1021 accaaacgtt cccactgcag caggagagtg ggcagacagt ggagtgtaca gtggcccagt
     1081 acttcaagga caggcacaag ctggttctgc gctaccccca cctcccgtgt ttacaagtcg
     1141 gacaggagca gaaacacacc taccttcctt tggaggtctg taacatagtt gctggacaga
     1201 gatgtataaa aaaattaaca gacaatcaga cctcaaccat gatcagagca actgccaggt
     1261 cagcacctga tcgccaagag gagatcagca aactgatgcg aagtgcaagt ttcaatacag
     1321 acccatatgt tcgtgaattt ggaatcatgg tgaaagatga gatgacagat gtgactgggc
     1381 gggttctgca gccgccctcc atcctctacg ggggcaggaa caaagcaatt gccacccctg
     1441 tccagggtgt gtgggacatg cgaaacaagc agttccacac gggcatcgag atcaaggtgt
     1501 gggccatcgc ctgcttcgct ccccagcgcc agtgtacaga agtccatctc aagtccttca
     1561 cagagcaact gaggaagatc tcgagagatg ctgggatgcc gatccagggt cagccctgct
     1621 tctgtaaata cgcacagggt gcagacagtg tggagcccat gttccgacac ctgaagaaca
     1681 catacgctgg cctccagctg gtggtggtca tcctgcctgg caaaactcct gtgtatgcgg
     1741 aagtcaagcg tgtgggagac acagtgctgg ggatggccac acagtgcgtc cagatgaaga
     1801 acgtgcagag gaccacgcca cagaccctat ccaatctctg cttaaagatc aatgtcaaac
     1861 tgggaggcgt caacaacatc ctgctgccac agggcaggcc tccagtgttc cagcaacctg
     1921 tcatcttcct gggagccgat gtcacccacc caccagctgg ggatgggaag aaaccttcta
     1981 ttgctgccgt cgtgggcagc atggacgcac accccaaccg ctactgtgcc accgtgcgcg
     2041 tgcagcagca ccgacaggag atcatccagg acctggctgc catggtacga gagttgctca
     2101 ttcagttcta caagtccacc cgcttcaagc ccacccgcat catcttctac cgcgatggcg
     2161 tctccgaggg ccagttccag caggttctcc accatgagct cctggccatc agagaggcct
     2221 gcatcaagct ggagaaggac tatcagccag gaatcacgtt catcgtggtg cagaagcggc
     2281 accacacacg cctcttctgc acagacaaaa atgagcgggt tgggaagagt gggaacattc
     2341 ccgcaggcac aaccgtggac acgaagatca cccaccccac tgagtttgac ttctacctgt
     2401 gcagtcacgc cggcatccag gggacaagcc gtccttccca ctaccacgtg ctttgggatg
     2461 acaatcgttt ttcttctgat gagctgcaga ttctgaccta ccagctgtgt cacacctacg
     2521 tgcgctgcac acgctctgtg tcaatacccg cgccagctta ctatgctcac ctggtggcct
     2581 tccgagccag gtaccacctg gtggataaag aacatgacag cgctgaagga agccatacct
     2641 ctgggcagag caacgggcga gatcaccaag cgctggccaa ggcggtccag gtccaccagg
     2701 acacactgcg caccatgtac tttgcttgac ctgttgtatt gtttagcgat tgtgtaccaa
     2761 gttggattca cacgagacca gctacactca gaccaacaga tgcccagccc ttctgtgaca
     2821 gccagcatcg aacatgagac gtctttggtt ttattagatc ctccgttttc cagaatgcct
     2881 tccgtcccag atgtcagact tggattttga gctgcaggcc tgtatgagac cccagtgtag
     2941 gaaacatggt ttgccaaacc tataagctgc tcactgagag tcccgtttcc taaaaacaaa
     3001 acaacaacaa aatgtcctaa aaccagtcta tgaactctca gggctttaaa acactttaat
     3061 ttatttggtc atccaattta cttgttttta acacctgatt ctcactgaaa ccgatgggct
     3121 ccatctagct gtgaaggctc tgagcatgaa agccacacca tttccattgg ggttttaaag
     3181 cctggcccta agaagttgtc actaatgttg agttctaagg gatgccatgc gccctgcagc
     3241 cccagggcgt atggctgcta ggtctccaca gtgtctctcg aggtcactgc ctgccctcga
     3301 ggcctgaaca aaggcacagt ggccaaggag atggtcacgt gtgtttacgg cagtgacaag
     3361 tccgagagat tggcagacaa gtgccatttt gataaaaatt tatttaaaaa gagaaaaaaa
     3421 gacaatatac tgacagtcta atcataagat ttgtcctgta ggactgtgac atttattttc
     3481 caaagtttct aaagaatacg ggtctgtggt gataaataca gtacaatcct ttttcactgt
     3541 taatgtcttt aatgtaagag aagaatatat gtatctggtt tgcagtatta atgtggtagg
     3601 tagatgctgt ttccctttcg ttgacgaggg gcgcttcttg tgacctgctt cacatatcag
     3661 gagctgtgtg gagcctggag acagtccacc tcttgtggct tttagtgctt aagtagagga
     3721 atatgcaact ggcttcatgt accagcgatg ccccctcctc cctccccacc cccagtgtgg
     3781 actgtgtagc ctgtacctct ggaaggacag tgcgcttgcc agtaacctgc cgtcctccct
     3841 gcctcgtcca tgtgtgtcgc tcagccgatg aagcacaaat ggaatttatt tattcttcca
     3901 ttgctgggaa acaagttcac gtaagagtgc acttctctca gtgtgcagtt atggaaggtg
     3961 ggtttcatag gatctatgca ctgaagtgga gagagagctg cccatagcgg ctctgccagc
     4021 atgggactgg caggtaggaa tggactgtgc ccgaggacct gaccgtgggc aaggccagca
     4081 gggaaggcac agactttctc tgggtgttgt ttgcttctgg gccccagtgt gggttttcca
     4141 gcccagagac cattgaactg agaaggggcc actacgagtg gctctgcgga tggcagcagc
     4201 cacgtcagtg tgtgtgtcct gtggggcttt ctgtgcttgt cccgaagctg agcaagcatc
     4261 gggctcgctc cgttccacat acgcgtgcgt tcccagaagg agacgagcct tctcttgtgt
     4321 gctgctctgt gctctgtggg ttttttctac ccctgctgat gggcctcatc ccccagctct
     4381 gcgtcattta cctctgaccc tgtggaggag ggagggtgcc caccctctcc cctgccatgg
     4441 cacctcatgc tagaggacac agtaccacct ttgccccgtt ccccatctgc tctggagctg
     4501 gtgggaaggg cagacgcagt tacctgggaa gtcacaccct cagcaggtgt gtgtatccgt
     4561 gtatgcatgt gggtgtctta ctaatgacaa tagtctcttc ttgctgtgtc tttagaaatc
     4621 ttttaaagta acaggacccc ccctcccccc cagtctaaga agcactggga ctgcctgcta
     4681 tcccattggc aagtggcacc ttctcaaact gtttccaaaa cgatagaaaa acactcagtt
     4741 ggcctaacat ggtcaggcta gggaactggt taaactagtg aacttttaga atctgaaatt
     4801 ggattgtttt caaccattct tagaaccgcg tgtttctaag gtgtgtcttc agccccttgc
     4861 catgggatct tagtcatgca agtgagtttt ttagaatgca gattctcacc tgctgccagc
     4921 ttcttggatc tggcactgtg ttcgaggcca cccctcaacc cccaccgtgg ctctgtaggc
     4981 gctttctgag gccgtccccc cttatgatgc cagggggaca ggaaggaacc cctatcagag
     5041 ctcacaccta ggaacacttg tgggctgggg caaaaaatct tgagctggga gttttgtttt
     5101 gatcaccttg aaggataggt tttctttctg atgggccaaa gtactggaag caaagtactt
     5161 tctgtagtag cccatgctta cttgtgacac ttttaatatg aaatgtatcc ccagaccaaa
     5221 tgtccctaat tgagttagat acacaccatt tacatgtctg atggtgcaga gctcataaat
     5281 ggctatagtt ccaatttctc aggtaaataa ttttccccaa ttcccgccct ccccttatta
     5341 ttaagattag tttcgagaca gtgtgagacc tcagagtgcg gtagcagctc cggccatctt
     5401 ctgcaggagc cagctttttg tgtgtatctg tacccaagac tggtgacagg tgccgttttc
     5461 tcctgaggtg atacctggcc aagtttgacg actgcccact tgctagctgt gttcccaacc
     5521 ccactgttaa gtaaggtgtg ccttgcttta agcaaactga atgtgattgt gttgtgctct
     5581 gtatccattt ggaaggaaaa ctaggatgtg cctgtctctt taagagaggc tacccaatta
     5641 gcctttcatg gtgggactct cctagtagta cactgtaaga tactcactct taaaaaccca
     5701 tcatgacatc catcccttta ccactgtgtg caccatgcag caggtccctc cctccccaag
     5761 ctgatgaagc aatgcaaaac tctagagaga catcgaggcc ccattccata gatttagccc
     5821 ttagtgatac tagcaaaact cccaccctgt cactgcgtgt tttaagccca gattctccta
     5881 gtccccatca agtctgaaac cgagcacgcc tgtacttcta gtcaggggct gttgggtttt
     5941 ggcttccttc tcccttttgg ggaaatcctc acatgcacac ttgataccac tttgggtctg
     6001 ctctgccttc tccagatttg gcctcttggc cactcctcac acccacccgc ttgctggcct
     6061 ccaggccaca gggcactgcc agtgaggaga ctgctagctc cgtgctgtct gcagctggcc
     6121 caacatctgt atcctaatgt ttacctgtca ccgagcggcg cttgatagaa agatggcccc
     6181 tgcagcgggc ttgggcaggt ttggtttttt tggttttggt tttgtttttc cattgtcttg
     6241 ttcttgtgtt attttattgg ctttaaatgc aaatgttgct atttattttt tcctctctct
     6301 cctgctctgg tttgactaag ctgatctggc tatcccaatg catgtggtac ctgatagatg
     6361 gatatggtag agattatttc ccctgccttt tataactgtc ccagtgttta ccttcaggct
     6421 tgctaatgct ttatttttgg aagccacaga tgtagttttt ctggatgaat tgcttttcct
     6481 atagatgcag cggtatgctt ttgcattgat ctgcagggtg atttgaattt tatttttaca
     6541 agcagttttt caatcaaatt ctatatttga tacaatgtgt taaggttaga ccagcccgaa
     6601 gtcatgcagc tggggacccc tgagtgtcca ggaggcctta gggctgggtg gcctctcttg
     6661 gtagtccctg tggtggtagc actggtcaca ggaacctgca gggtccttca gcatggccct
     6721 gaactcttgg attgttacaa ggattttttt tttttcatgg gcattttgct acacagacag
     6781 tggacaatcc cggtggcccc agacaagcca taagcaacct ttctgtgacc tccccctgac
     6841 tcatctctca cacccatgag aaaaggaatg tgccctgagt agaagctaca cttccttggt
     6901 gttcatctgc ctcacttgtg aatgcagata ttttgtttgt tttaagccct tccctgcagt
     6961 aaccatgctg gctggctctc acataggcag agcacacgtg actcttgtca caagcgcccc
     7021 gttgcaggca tgccgactcc tgtaagttgc actgatctct caacagtttc ctttattgcg
     7081 tccaactccc catctgctgt actcatttga agtgattgta aacagattca cagtggcaag
     7141 agtgggacat tgcctcacgt ctgccatccc aacacacatt tcagtattac atgctcgagt
     7201 gtgcgggctg ggctcttact ctctcgagca ttgtgggcta gccttgtctc aacactctga
     7261 caagaagttc tccctctcta tacaggacag catgctcttt gagacctgaa ggcttgaatg
     7321 taacagatta gttaggagtc tttgtggcgc tttggtctgt ttagttttct tgtgatctgc
     7381 aaggcccccc aaaagtatgt ccttctagtt ccgtgtggcc tttgtcagca tggacagcat
     7441 atgaatagcc cagtcagtgt taacaggcca ttgctacatt ctttggtcag cccctcaact
     7501 taaagttgtg aggtcactgc tgtgtcagac tgtcatttgt ggtccatcca tgggagacct
     7561 aaagcctggt gcagcccttt atgccatgtg accttgctgg gacctaagta catggtctgt
     7621 ttagccccac gtggactgtc tgtagtcagg gtgcccagag tctgaaacaa aaggaattat
     7681 tacactcaaa ccgcggggga ggggggtact ggggacggac ggacagacag acagacagac
     7741 agacagacag acagacagtg ccattgcaga cattgcagag ccctccctca ccttgtgtct
     7801 ttggatttgt tcagccattt tatttgtctc aagttgaatg taccggctta gccaaatcac
     7861 tacagaaggt ggtttcttta aaccttttag acagatgcat aaaagctacc tctgacttct
     7921 catagatttg tgtttgctct gaagcatcgc tatccacact gaatatagcc aaaatatcgc
     7981 ctgaataaac cgagttacga actcctctgt tcagttctgg cttgaaaatg tgtgagtgcc
     8041 atactgtgat ctggggccgc agctcctctg ctgccactga cccagggact cctcagcacc
     8101 acccagctgc aagatcctag cgtccgagag gctggtagac tggcttctct gcatggggaa
     8161 cgtgcaccct ccaggctggc gggcgtgact gcgcagggcc gcgtgcggct gagcctggag
     8221 atggtctgtt cctgcctggc tttgctttcc tgttgaatcg tgtaagaagg catctttacc
     8281 cctgcttctg aatggattga aagtggcttt cctgctctct cctccctttt tgtacactat
     8341 aaactcataa aggtgcttca acaccgactc ttgtgcagat aatgtagtta agcagttgca
     8401 tggattcaga tagtgcaggc acagctgacc ttgggaccct ggtgaaagtg aaggactgaa
     8461 agccctgcgc tccatttgga aggagcaatg ggctctgctt gactagaagg tccctttagt
     8521 gttggcagaa cagagatttg tcatggggac ctgccacctc tgaccaagct gtcctgtggc
     8581 tgtctgcttt gctgtccagg aggtcatcct cttacatcat ggctccacct gcatctcctt
     8641 ggcctctcca cctaaagttt ccttggaaag tcagcataag atcccagatt tccctgttta
     8701 gcttgaggga cagatggaca gtaccatgaa gggacagatt gacagtgtcg tgaaggtcgg
     8761 tgcctgggga ttgggctggg cttaggggag ctcttctaaa ggttgtctct gcgccctccc
     8821 caacctcccc cacatacacc cccattgtat tgttttgggg ggtgtcatct tccattcttt
     8881 tgacttggac agctcagtac ccttaagagc cctcacatat ttctctgtgt agagaacctg
     8941 aggcgtggag ggtggtgggg tgtctgcaac acccctgacc tgcaccccag gttacttaaa
     9001 tcccctgact tggctggaat ttgaaaatcc agggaagcac agggtgctag atatgttaca
     9061 cttagggtca cacccaggtg ccgtggtgta agccacagcc ctcagggcac cagcacagag
     9121 gcagccagcc tccccactcg tgacatttgg gaaggatact aaacaagcca gagccctggc
     9181 cgctgcagta ttcagctaac acttcttttt cccggttgaa aggagaacac tatttgaaac
     9241 cttctaatgc cctggtggtt ctagttcctc cgttcctgag ggctaaaagg ttgaatgagc
     9301 tcctgttttg cttagagctg cctttatttg agtccattta aaggtgaagc acgggactgt
     9361 ctcctctccc tgagtcacca gcaagtaggt gcggagaccc ttctcattca gtcctttctg
     9421 tatcttcaag cgccccaaca tcagtgggat cacgagagca cccacatcag ccatcagtgg
     9481 agtcaagtat acatatgggg acagaaattg gcaaatttac aggttgtaat ggccactcca
     9541 gcctgtgaca gggcaggccc tccatcctgc acagcaggtg gcctctgtcc tacctccagg
     9601 cactggcttt actatctgga cacctagttg agagccttga aagtctgttc ttggtgtcct
     9661 tttagaatca tgctggggct ggtcgttagt tgaccctgcc cagaagctgt cagccctcag
     9721 ctctttcttg caaactgtga tgccaccttg tagcctacgg ctgtggagcc ccgtcctggg
     9781 ttctttgtgc cctgaaatgc aggtgcacct ctgctggggg ttggtggggc actggcaaag
     9841 gctacttgct ggccttgtgt tctctgcccc tcagcaagac tgtctgttgt agtggaagta
     9901 ggtttagagg gatttggaat catgtctttg tgaatcgagt gtgacgtcat caggggtacg
     9961 gtgctacttg gtcctaatgt tttggaatgc ttttccgtcg ttgttactcc attgttactc
    10021 tctgttctat gacactgagg gagactgctc acttaggtgt ggtgtcacat aggactccag
    10081 ctttgatgtg ctgtgcgggt ggtgtgatta tcatctctga ccttctgaag ccaggggtgg
    10141 tacccatccc tagctcagtc taattaaatc tttcctccct gtcaagtgat tgctttgggt
    10201 ggttttaaca ggagtagtcc agtctggata ttctattttc ttaattacat agaaaaggcc
    10261 cgtgcagcac tagctgcaga cagataacac atttaacaat tttggttcct cttcaaagcc
    10321 aaatagacct atgttgatta aactccaaga gggtacaatt cagcattaaa ttaaaatttg
    10381 agttgtgaat ttgagaggga atttcagtat agttgataaa gttaagtggc aagtgagtat
    10441 ttataaaagt tataagagaa taattgtaag tttctgtaca acaatttggc tactgcctca
    10501 taaatacagg aagagaccgt agccctgaat ccgactctct cacctcccac ataccttgga
    10561 ctacctgcct aatatgtatt tgagggggtt taacccttgg ttttatcaaa ttttctagtt
    10621 ttattttatt tttataaaat aattgaaaaa gaaccaaaaa ggaatccaga agttccccag
    10681 tggctcctcc cccagtctta atcccctccc ccccacacac acacacacac tcaaatgttt
    10741 tagaagacat tttgaatcaa aatgtattcc acccatttaa cgtttgctac tttctgagaa
    10801 gtggagtggc tacgagggcc tgtttcagat ggtactctgt atgtcatgga catgtttgta
    10861 cctgtacagt ggatctgtgt gacgacgtct tgtgcttgcg gtttagaaac cacgcagtgc
    10921 tgttttgtaa attgacagtt tctgtgtaaa ctattattta tatcccttgg cccctctgta
    10981 taatttttca tttgctacat ggattgtata attaattata aaattatagt acctgctaaa
    11041 cccaattctg aggcttcatg tcattgtctt tggtttgata ccagtctgat cgggaggctg
    11101 cccaggcctt gcgcaatacc agtagtgatg ttacttaaag ttaagcctta atcagacagt
    11161 gaatttgtgc atttcagagt atgcaaaaaa aaaaaaaaaa aaaaaagcag agtgagtttc
    11221 aactgcccag gtcaagatgt tcaaccctaa ctaacaggtt ccttctggag tgaaaaagtc
    11281 taatgggttc cctctggggt gaaaaaaggg ccgttacccg ggttacactc ttccctccca
    11341 cctgcgctct cccatgtggc gtgatgaaac tgatcgtgtg catttctgtg gcagtctatc
    11401 tagagacctt tgcagggttg tcttcaaatg tctggtttct ctcctctggt ctgtgacgag
    11461 cgagcgtcta ctgccgggag cacagctggg gcgtaggttt caccaacctg ctcagcggcc
    11521 aagctctccg ctgttactgc cacctgctat tgcttctgca tagattataa cagatttgtg
    11581 tcgggtaaaa gcatcttcaa tcttgaggta aattagaggg gacccagctt tcttttacaa
    11641 tgtggatcta cctcagttaa acagttgggt gctattaact aagtctgtca aattaaattg
    11701 gaaagtaacc aaacagtggg acataactaa ctccacgtga aacttgaaga catcatttcc
    11761 gtttctttaa taatactttt tatttatttt gtgcctttta tgttaaccct aatgcgtttt
    11821 ttaaaattca gaatagtcat ttttctcttg cgtgtgggga gcccaactga ccatacaagc
    11881 agccatctaa ctgctagagt atactgggat cctaacctac cctcttggga aacggagggg
    11941 gaaaatgtgt ccaagagcaa atttcacaca tcacataccc cagctcttac atgcttacct
    12001 tcatagcttt cgcttcattt ctgtgccatc tgaaggggca acccccaaaa ctgacactcg
    12061 agtgctttgt ctctgctttt tcagctgtcc gtgtctctct tctctggcat gttttctata
    12121 agactttctt tcataaacag caagtggact gttgtcgatc ccatctcctg gcttgcacag
    12181 tgaaggtgct aaacgccagg agcagaggcg ggggcaagct cacggttgtc tggaggccag
    12241 gccggaggcg gggaggatgt gcacgatgcg gcagcccgcg gtttatgaag tcaggaacac
    12301 tctggtaaga aaataagatc ggagatcgat aactttccat gcctttatat ttggcagttt
    12361 acaacttttc ctactacggc aacctttttg ccataattct ttggaggtaa cacaatattt
    12421 tgaagtctta tgaatttgat tttataccag tgagaagtca aagttttagc ggtctttcct
    12481 gtagtgtatg gttttactct tttaaagtca tctctggatt ttagaagaga acctattgta
    12541 cttggtggct ccaaaaggtg atttggggag ccctgtccct tcccttcttg ctctgctccc
    12601 ccccccccct ttccctttgc agggtctcaa aaaaggaaaa caagaaaaaa aaatgatggt
    12661 tctagttaga tttgtggggg ttttttattg ttggtttttt tttttaagct tccatcaggt
    12721 tgatacttaa gctatatttt atagaataaa tcccccttta gtatgctgca tatggatatg
    12781 aagctcagag ctgttgcact ttctctgtaa tcaatgccct ccaactggct cgctggaagc
    12841 attttctttt aacctaatgt gtactttggg caggtgatga aatttacaca ggtacctcaa
    12901 gtcaagacct gcgcgtgctg catttggatt tttggttttg ttttgttttg cttttctatg
    12961 tatcattatg ttttacttaa ttttgttctg gtgttatgct ttttggaccc caatagtctg
    13021 ttgaaagaaa aacctaaaaa gttagtatcc ttgttttctc ccccctgcag tatgtggttc
    13081 taaaggtcac cccccccccc cattaggaat ctcccccaag cacacccgag gaaaagggta
    13141 cgacgggcgc ttctcagcgg gccgtgccat tgagatcttt tatattcatg ctgcttctag
    13201 tcccttctcc acatcaccta agggtgggta cctccattgc gatgttgtgg catcttaact
    13261 tttacacaca gacctcatgg gtgtccactg aggaagtagt atgagcctaa agacactggc
    13321 agcgtgcagc ctggcctggg agtggcggcc ttcgcatctc tctctctctc agcactcagc
    13381 cacgctgacc tggccgggtg gtccgcgtgg acaggacagc tggtgaagca agccggatgc
    13441 tcagaacact gcgcgtctcc tgtcctgttt taagcttagc tgggtccttt gttttcacag
    13501 cttcctaggt taaatagttt gcactatttt tgcaataaat tcatgggaaa cctaatggct
    13561 acttatgttg ttgttcttac tgtgtgtata aactaacact aaaggtttgc tatagtgttt
    13621 tcttttcacc tccttatgta gccccataac atgcacagaa tgctgtaaac ctgattaaga
    13681 tatgatgtgg atattttata aagttatttt gtatggtgtc gattttgttt tgccgcatag
    13741 tgtgtcagca aaaaataaaa taaaataaaa ttcctcgtat ttacagctgc cta
//
