LOCUS       NM_153403               7255 bp    mRNA    linear   ROD 13-MAY-2024
DEFINITION  Mus musculus argonaute RISC catalytic subunit 1 (Ago1), transcript
            variant 1, mRNA.
ACCESSION   NM_153403 XM_994455 XM_994480
VERSION     NM_153403.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 7255)
  AUTHORS   Liu,Q., Pepin,R.M., Novak,M.K., Maschhoff,K.R., Worner,K. and Hu,W.
  TITLE     AGO1 controls protein folding in mouse embryonic stem cell fate
            decisions
  JOURNAL   Dev Cell 59 (8), 979-990 (2024)
   PUBMED   38458189
  REMARK    GeneRIF: AGO1 controls protein folding in mouse embryonic stem cell
            fate decisions.
REFERENCE   2  (bases 1 to 7255)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 7255)
  AUTHORS   Muller,M., Fah,T., Schaefer,M., Hermes,V., Luitz,J., Stalder,P.,
            Arora,R., Ngondo,R.P. and Ciaudo,C.
  TITLE     AGO1 regulates pericentromeric regions in mouse embryonic stem
            cells
  JOURNAL   Life Sci Alliance 5 (6), e202101277 (2022)
   PUBMED   35236760
  REMARK    GeneRIF: AGO1 regulates pericentromeric regions in mouse embryonic
            stem cells.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 7255)
  AUTHORS   Zhang,Y., Tan,Y.Y., Chen,P.P., Xu,H., Xie,S.J., Xu,S.J., Li,B.,
            Li,J.H., Liu,S., Yang,J.H., Zhou,H. and Qu,L.H.
  TITLE     Genome-wide identification of microRNA targets reveals positive
            regulation of the Hippo pathway by miR-122 during liver development
  JOURNAL   Cell Death Dis 12 (12), 1161 (2021)
   PUBMED   34907157
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 7255)
  AUTHORS   Eswarappa,S.M., Potdar,A.A., Koch,W.J., Fan,Y., Vasu,K.,
            Lindner,D., Willard,B., Graham,L.M., DiCorleto,P.E. and Fox,P.L.
  TITLE     Programmed translational readthrough generates antiangiogenic
            VEGF-Ax
  JOURNAL   Cell 157 (7), 1605-1618 (2014)
   PUBMED   24949972
REFERENCE   6  (bases 1 to 7255)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   7  (bases 1 to 7255)
  AUTHORS   Carmell,M.A., Xuan,Z., Zhang,M.Q. and Hannon,G.J.
  TITLE     The Argonaute family: tentacles that reach into RNAi, developmental
            control, stem cell maintenance, and tumorigenesis
  JOURNAL   Genes Dev 16 (21), 2733-2742 (2002)
   PUBMED   12414724
  REMARK    Review article
REFERENCE   8  (bases 1 to 7255)
  AUTHORS   Beier,H. and Grimm,M.
  TITLE     Misreading of termination codons in eukaryotes by natural nonsense
            suppressor tRNAs
  JOURNAL   Nucleic Acids Res 29 (23), 4767-4782 (2001)
   PUBMED   11726686
  REMARK    Review article
REFERENCE   9  (bases 1 to 7255)
  AUTHORS   Cikaluk,D.E., Tahbaz,N., Hendricks,L.C., DiMattia,G.E., Hansen,D.,
            Pilgrim,D. and Hobman,T.C.
  TITLE     GERp95, a membrane-associated protein that belongs to a family of
            proteins involved in stem cell differentiation
  JOURNAL   Mol Biol Cell 10 (10), 3357-3372 (1999)
   PUBMED   10512872
  REMARK    GeneRIF: describes cloning rat sequence and used RNA interference
            to show that the GERp95 orthologue in C. elegans is important for
            maturation of germ-line stem cells in the gonad.
REFERENCE   10 (bases 1 to 7255)
  AUTHORS   Araki,K., Imaizumi,T., Sekimoto,T., Yoshinobu,K., Yoshimuta,J.,
            Akizuki,M., Miura,K., Araki,M. and Yamamura,K.
  TITLE     Exchangeable gene trap using the Cre/mutated lox system
  JOURNAL   Cell Mol Biol (Noisy-le-grand) 45 (5), 737-750 (1999)
   PUBMED   10512203
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from CJ130124.1, BC129914.1,
            AK080954.1, CV559803.1, AL606976.25 and AI451331.1.
            
            On Nov 19, 2015 this sequence version replaced NM_153403.2.
            
            Summary: This gene encodes a member of the argonaute family of
            proteins, which associate with small RNAs and have important roles
            in RNA interference (RNAi) and RNA silencing. This protein binds to
            microRNAs (miRNAs) or small interfering RNAs (siRNAs) and represses
            translation of mRNAs that are complementary to them. It is also
            involved in transcriptional gene silencing (TGS) of promoter
            regions that are complementary to bound short antigene RNAs
            (agRNAs), as well as in the degradation of miRNA-bound mRNA
            targets. Alternatively spliced transcript variants encoding
            different isoforms have been found for this gene. A recent study
            showed this gene to be an authentic stop codon readthrough target,
            and that its mRNA could give rise to an additional C-terminally
            extended isoform by use of an alternative in-frame translation
            termination codon. [provided by RefSeq, Nov 2015].
            
            Transcript Variant: This variant (1) represents the predominant
            transcript and encodes two isoforms, which result from the use of
            alternative in-frame translation termination codons. The shorter
            isoform (1) results from translation termination at the upstream
            UGA stop codon, while the longer isoform (1x) results from UGA stop
            codon readthrough to the downstream UAG termination codon. This
            RefSeq represents the shorter isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC129914.1, AB081471.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-162               CJ130124.1         2-163
            163-2736            BC129914.1         1-2574
            2737-3224           AK080954.1         2425-2912
            3225-3556           CV559803.1         331-662
            3557-7095           AL606976.25        152062-155600       c
            7096-7255           AI451331.1         2-161
FEATURES             Location/Qualifiers
     source          1..7255
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 60.76 cM"
     gene            1..7255
                     /gene="Ago1"
                     /gene_synonym="Eif2c1"
                     /note="argonaute RISC catalytic subunit 1"
                     /db_xref="GeneID:236511"
                     /db_xref="MGI:MGI:2446630"
     exon            1..187
                     /gene="Ago1"
                     /gene_synonym="Eif2c1"
                     /inference="alignment:Splign:2.1.0"
     CDS             163..2736
                     /gene="Ago1"
                     /gene_synonym="Eif2c1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     Piwi/Argonaute family protein meIF2C1; argonaute 1;
                     protein argonaute-1; mAgo1; eIF2C 1; eIF-2C 1; argonaute1;
                     eukaryotic translation initiation factor 2C, 1; argonaute
                     RISC catalytic component 1"
                     /codon_start=1
                     /product="protein argonaute-1 isoform 1"
                     /protein_id="NP_700452.2"
                     /db_xref="CCDS:CCDS18653.1"
                     /db_xref="GeneID:236511"
                     /db_xref="MGI:MGI:2446630"
                     /translation="MEAGPSGAAAGAYLPPLQQVFQAPRRPGIGTVGKPIKLLANYFE
                     VDIPKIDVYHYEVDIKPDKCPRRVNREVVEYMVQHFKPQIFGDRKPVYDGKKNIYTVT
                     ALPIGNERVDFEVTIPGEGKDRIFKVSIKWLAIVSWRMLHEALVSGQIPVPLESVQAL
                     DVAMRHLASMRYTPVGRSFFSPPEGYYHPLGGGREVWFGFHQSVRPAMWKMMLNIDVS
                     ATAFYKAQPVIEFMCEVLDIRNIDEQPKPLTDSQRVRFTKEIKGLKVEVTHCGQMKRK
                     YRVCNVTRRPASHQTFPLQLESGQTVECTVAQYFKQKYNLQLKYPHLPCLQVGQEQKH
                     TYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLMKNASYNLDPYI
                     QEFGIKVKDDMTEVTGRVLPAPILQYGGRNRAIATPNQGVWDMRGKQFYNGIEIKVWA
                     IACFAPQKQCREEVLKNFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFRHLKN
                     TYSGLQLIIVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVVKTSPQTLSNLCLKIN
                     VKLGGINNILVPHQRSAVFQQPVIFLGADVTHPPAGDGKKPSITAVVGSMDAHPSRYC
                     ATVRVQRPRQEIIEDLSYMVRELLIQFYKSTRFKPTRIIFYRDGVPEGQLPQILHYEL
                     LAIRDACIKLEKDYQPGITYIVVQKRHHTRLFCADKNERIGKSGNIPAGTTVDTNITH
                     PFEFDFYLCSHAGIQGTSRPSHYYVLWDDNRFTADELQILTYQLCHTYVRCTRSVSIP
                     APAYYARLVAFRARYHLVDKEHDSGEGSHISGQSNGRDPQALAKAVQVHQDTLRTMYF
                     A"
     exon            188..371
                     /gene="Ago1"
                     /gene_synonym="Eif2c1"
                     /inference="alignment:Splign:2.1.0"
     exon            372..492
                     /gene="Ago1"
                     /gene_synonym="Eif2c1"
                     /inference="alignment:Splign:2.1.0"
     exon            493..674
                     /gene="Ago1"
                     /gene_synonym="Eif2c1"
                     /inference="alignment:Splign:2.1.0"
     exon            675..811
                     /gene="Ago1"
                     /gene_synonym="Eif2c1"
                     /inference="alignment:Splign:2.1.0"
     exon            812..946
                     /gene="Ago1"
                     /gene_synonym="Eif2c1"
                     /inference="alignment:Splign:2.1.0"
     exon            947..1034
                     /gene="Ago1"
                     /gene_synonym="Eif2c1"
                     /inference="alignment:Splign:2.1.0"
     exon            1035..1182
                     /gene="Ago1"
                     /gene_synonym="Eif2c1"
                     /inference="alignment:Splign:2.1.0"
     exon            1183..1302
                     /gene="Ago1"
                     /gene_synonym="Eif2c1"
                     /inference="alignment:Splign:2.1.0"
     exon            1303..1425
                     /gene="Ago1"
                     /gene_synonym="Eif2c1"
                     /inference="alignment:Splign:2.1.0"
     exon            1426..1559
                     /gene="Ago1"
                     /gene_synonym="Eif2c1"
                     /inference="alignment:Splign:2.1.0"
     exon            1560..1744
                     /gene="Ago1"
                     /gene_synonym="Eif2c1"
                     /inference="alignment:Splign:2.1.0"
     exon            1745..1904
                     /gene="Ago1"
                     /gene_synonym="Eif2c1"
                     /inference="alignment:Splign:2.1.0"
     exon            1905..1995
                     /gene="Ago1"
                     /gene_synonym="Eif2c1"
                     /inference="alignment:Splign:2.1.0"
     exon            1996..2190
                     /gene="Ago1"
                     /gene_synonym="Eif2c1"
                     /inference="alignment:Splign:2.1.0"
     exon            2191..2325
                     /gene="Ago1"
                     /gene_synonym="Eif2c1"
                     /inference="alignment:Splign:2.1.0"
     exon            2326..2427
                     /gene="Ago1"
                     /gene_synonym="Eif2c1"
                     /inference="alignment:Splign:2.1.0"
     exon            2428..2627
                     /gene="Ago1"
                     /gene_synonym="Eif2c1"
                     /inference="alignment:Splign:2.1.0"
     exon            2628..7227
                     /gene="Ago1"
                     /gene_synonym="Eif2c1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7197..7202
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ago1"
                     /gene_synonym="Eif2c1"
                     /note="hexamer: TATAAA"
     polyA_site      7227
                     /gene="Ago1"
                     /gene_synonym="Eif2c1"
                     /note="major polyA site"
ORIGIN      
        1 ggcggcaacg gaggctgcgg gggcgacagc gcgagcggcc gggcttcggg ggggagccga
       61 gcccggcccg ggagcccgag cagtgcaagt gcgaggtacc taggcccctc acgctggact
      121 cctcagtctc ccggccgcct gtcctccgca cgggtatatg ggatggaagc gggaccctcg
      181 ggagcagcgg caggtgccta cctgcctccc ctgcagcagg tgtttcaggc accccgccgg
      241 cctggcattg gcactgtggg caaaccaatc aagcttctgg ccaattactt tgaggtggac
      301 attcctaaga ttgacgttta ccattacgag gtggacatca agccggataa gtgtcctcgc
      361 agagtcaacc gggaggtggt ggaatacatg gtccagcatt tcaaaccgca gatctttggg
      421 gatcgcaagc ctgtgtatga tggaaagaag aatatttaca cggtcacagc actgcccatt
      481 ggcaatgaga gggttgactt tgaggtgaca atccccgggg aggggaagga tagaattttt
      541 aaggtctcca tcaagtggct agccattgtg agctggcgca tgctgcatga agccttggtc
      601 agtggccaga tccctgtgcc cctggagtct gtgcaagccc tggatgtggc catgaggcac
      661 ctggcatcta tgaggtacac ccctgtgggc cgctccttct tctcaccgcc tgagggctac
      721 taccacccgc tggggggtgg gcgcgaggtc tggttcggct ttcaccagtc tgtgcgccct
      781 gccatgtgga agatgatgct caacattgat gtctcagcca ctgccttcta caaagcacag
      841 ccagtgattg agttcatgtg tgaggtcctg gacatcagga acatagacga acaacccaag
      901 cccctcacgg attcccagcg tgttcggttt accaaggaga taaaaggcct gaaggtggaa
      961 gtgacccact gtggacagat gaagaggaaa taccgcgtgt gtaatgttac ccgccgccct
     1021 gctagccatc agacgttccc cttgcagctg gagagtggac agactgtgga gtgcactgtg
     1081 gcacagtatt tcaagcagaa atataacctt cagctcaagt atcctcacct gccctgccta
     1141 caagttgggc aagaacagaa gcatacctat ttgcccctcg aggtctgtaa cattgtggct
     1201 gggcagcggt gcattaagaa gctgactgac aaccagactt caaccatgat aaaggctaca
     1261 gctaggtcgg ccccagacag acaggaggag atcagccgcc tgatgaagaa tgctagctac
     1321 aacctggatc cctacatcca ggaatttgga atcaaagtga aggatgacat gacggaggtg
     1381 acaggacgtg tgctgccggc gcccatcttg cagtatggcg gccggaaccg ggccattgct
     1441 acgcccaacc aaggtgtctg ggacatgcgc gggaaacagt tctacaatgg gattgagatc
     1501 aaagtctggg ccatcgcctg cttcgcaccc cagaaacagt gtcgagaaga ggtgctcaag
     1561 aacttcacag accagcttcg gaagatttcc aaggatgcag ggatgcccat ccagggtcag
     1621 ccatgcttct gcaaatacgc acagggggca gacagcgtgg agcccatgtt ccggcatctc
     1681 aagaacacct actcaggact gcagctcatt atcgtcatcc tgcccgggaa gacgccagtg
     1741 tatgctgaag tgaaacgtgt cggagataca ctcttgggaa tggcaacaca gtgtgtgcag
     1801 gtgaagaatg tggtcaagac gtcaccgcag actctgtcca acctctgcct caagatcaat
     1861 gtcaaactcg gtggcattaa caacatccta gtcccacacc agcgctctgc tgtttttcaa
     1921 cagccagtga ttttcctggg agcagatgtt acacatcccc cagctgggga tgggaagaaa
     1981 ccatctatca cagcagtggt aggcagcatg gacgcacacc ccagccgata ctgtgccacc
     2041 gtgcgtgtgc agcgcccacg gcaggagatc attgaagact tgtcctatat ggtgcgcgag
     2101 ctgcttatcc agttctacaa gtccacccgc ttcaagccca cccgcatcat cttctaccgc
     2161 gacggcgtcc ccgagggcca gctgccccag attcttcact atgagctgct tgccattcga
     2221 gatgcatgca tcaaactgga aaaagactac cagcctggga tcacatatat tgtggtgcag
     2281 aagcgccatc acacccgcct gttctgtgct gacaagaatg agcggattgg gaagagtggt
     2341 aacatcccag cagggaccac tgtggacacc aacatcaccc acccatttga gttcgacttc
     2401 tatctgtgca gccatgcagg catccagggt accagccgac catcccatta ttatgtcctt
     2461 tgggatgaca accgtttcac agcagatgaa ctccagatct tgacatacca gctgtgccac
     2521 acttacgtac gatgcacacg ttctgtctct atcccagcac ctgcctacta tgctcgcttg
     2581 gtggctttcc gggcacgata ccacctggtg gacaaggaac atgacagtgg agaggggagc
     2641 cacatatcag ggcaaagcaa tgggcgagac ccccaggccc tggccaaagc tgtgcaggtt
     2701 caccaggata ctctacgcac catgtacttc gcttgaaggc agaacgctgt tacctcactg
     2761 gatagaagaa agctttccaa gccctgggag ctgtaccacc caaatccaga ggaagcaagg
     2821 cagagggagg tggggtcgga aggagtatag gaggccttgt ttctatctac agagggggta
     2881 taagggtggg aagaggacca gcaagacaga ctaccagcca gaaatcactg atagcaacct
     2941 cattgtcctc aacccctctc cccatcttgt catatccgac ccccttggac caaaaggggc
     3001 atcactagta cccaccatac acacagttgt ctcatgtgac tcacagtgct aaagactcat
     3061 gctcaacagc ctgctagggt cagctctgca gccctgtggt caaatgctgg tatgtttggg
     3121 tttgctattc tagatgagaa agtgggtggg aagagggcag gaatttttag gagccttaat
     3181 cagaaaaggt ctagatttgt ttaagaaaaa ggaaaacaga ctcagatcaa tattttagga
     3241 tactagatgt tttcatgggt tcagaatcca ctttgtagga agatttttaa tgctaatggt
     3301 ttgggttgct cctccccagc tgccatcccc tcccatcatt cctctccaca ttctctaccc
     3361 catgcacctt ttccctgaca gacatccagc cctcgtaaca cttaaggcac tatggcactt
     3421 agctctgagg tgacatgacc ctgtcttcct tccgcctgct ggtgggtaac cagtgccttc
     3481 ccgcaacggt aatgctgcag aactgcaacc ttttgtacct ttctctgtgg ggtggggtgt
     3541 gggtgggaaa ggagggaggg agggagggca gaaatagccc aatgccagcg agcctccagc
     3601 cagaatgcca gctaattttc atttgaagga attgacttcc ataatcattg agcttttaaa
     3661 aaaaggtcac aacctcaaga tggttaaaat ccattgacat ttgcactttc agacgtgaca
     3721 agtctccgag ctgctgagat gactggccct tggcctttcc cctttagctt cctcttttcc
     3781 ctggccccac caccaccctg ccagatctag agttactgtc atagtatttc tcattcttgg
     3841 cttttttacc cctgatggta aagtctcgtt tcaatatttc ttaactgggg tgtcttacag
     3901 cagaagatcg taaggactaa aataagaaaa caaaaaatct aaaggttatt tttataccat
     3961 aacttgagtc tattgccaaa atctggaaat tccctcccat acctgatgag tctgcattcc
     4021 agaaacatgg agccgtaaga gggaactgtg gaactgactt gtgttctggc ctagctaagt
     4081 agggggtggt tgtctcccca aggtggggct cagcctctgt ccttcctctg tgcagacaaa
     4141 gccttgttct cgagacagcc tccctccagg gcgctgtcct gccctcttcc ctgtaagtgc
     4201 atcttcccct tttctggact ggactgtcct ttgatccctg actaaccgag actctagcac
     4261 gtcctctgga caggctgggg aattttgctg ctcccatttg cttctgttta cacaaatgaa
     4321 attttcctgt ttctcactag ggcatgtgag tgggtggcat gggcttttct ttttctaccc
     4381 ctctcttgag gttctggggt tcagctagct gtcttgcagg actggaaaag aagtgggttc
     4441 tagctttgtc tttgctgacc ttgaagcaag gccccctctt tccttgacca ttcttttttc
     4501 ttcctgcatt attgcttggg atgggaaatt tgaacctccg tgtacaggtt ccccttctga
     4561 ggagcctgag cttaggattg ccctactctg gcgatgaagc catgatatgt tagctctagc
     4621 ccaggcttag aggcccgctg gagaaaggaa gatctgaacc ctggcctatg gagtcaaaac
     4681 ctttccccaa gagcccatgt atagacccag atttgctatg caaatagtcc agattcagtt
     4741 atggctggct acattattca gtaacttccc aacaggtagc acaaatattc cttatggaaa
     4801 aagcccagga ctgttcagta gttcctcctg tacttttctg ctggctacag tatggagtgc
     4861 cccatgggca caggcccagc cggagaacag aacggagggc tctgggaaga ggcagctcac
     4921 tggagagcct acattcctta cacaagtgcc taaacagagt gatgccaaca ctccatctgc
     4981 cctgtccatc gccttcatag acagtctact tcgtgttcca tcaccctttg gggagggagc
     5041 tctttgcaga cagtgtgttg tcaacttggg tacatttggg ggcttggatt aagggcacta
     5101 ctccaggagg gtccaatcat tcaccagcat ttgcagatgc ccatagggaa caggtggctg
     5161 tcacaactcc cagcaacaag attttgtttt ctcctttttc tttgcctcac tctctccagt
     5221 tgggttttaa gctagaatga aaagcagttt cggtcctttg gtatttatgt gccattcatg
     5281 aaataaaagc aagagaatgc tgggcacgat ggcccatgtc tgtaattctg ttatctcagc
     5341 actaaggctg aagcagaaga tttgcaagtt cagggtccac ctgggctact cagtgaattc
     5401 caggccagtg tgcactatgt agcaagacca tgtctccata aaaaagacca agaaaaatga
     5461 cttgggtgac agatcttgct gtgattttaa ataattcttt gagacttaac tactgaccat
     5521 gtgggttctt ctcatttaaa ggcagtatgc acacattaca aacagtgttg ccaagcctca
     5581 gcttgtttgg atttggttta agtggtgcat tgtaccgagc tctgggagga ttccagcctc
     5641 atcttagaca gctctttctc ctcccccaac tccctcccat gcctccaccc cacccccact
     5701 gttcggcttt ccctttcctc ttctggcctt ccctggcttc cttttccttc tccctcttcc
     5761 ctcagcttgc ttgctggctg gcctgcctgc ctgtctgcct gtgtgataat gaattctctg
     5821 catggctgca atgatcccac tgttagttgg caaggtcagg cttagctcat tgactacaga
     5881 agaccaaaat cctgtttctc atgcccagtg gtgttagccc tgggttgcac tgtcctcaag
     5941 cttacactag gtcagagcag caggcctgtc aaactgacaa aactagtagg gtttctggca
     6001 ggttcaacac ctctccattc tgtggagggg cttggagaag aggcagtgaa ggagaccgct
     6061 gctttgaatt cagtccagtg tgaccgattg gttgaggtgc tattggcagc ctgttggggt
     6121 cggcctggag ctactgttac tgggaactta aacttggact ttgactaaag ttgaacagag
     6181 tatgaaactg ttctagaaat ctagttctca actgcgacat agctcctctg tgactgagca
     6241 gcaaggcttt ccaacaggtt ccttttaacc agaattctat gaatgatgcc ggggacacag
     6301 ttttcttagc tggcccatag aaggtctaca aggctgctaa gtgttgtaga cctggcatct
     6361 ggcatcacat aggaggatgg aatttcttcc ctttcaggat agaagagtca cctcttacct
     6421 agccaaaagg cagttttggg aattcctgtc aggaggaaag gtttggtttt gttttttaat
     6481 ttactgggct ttagatggta gttcattatg ccttccttgg gcagggatca tggcagggat
     6541 acgcaaggca ctctaatact cggccttcta agcaggctgg gaaaggggga ttctctggtt
     6601 ttctctttct ccccacttac taggacttgc cttctccctg agccacgaga gaggctgggt
     6661 tggtgctctc cccttactct gctcctactg acttagaacc tctggctgct gtatgatagc
     6721 ccaggaaagg gaatggaaag aatgggctca aaacaataat gtaaggaagg tggtttcctt
     6781 ccttaaaaag ggaaatcaga aactctccaa gaattgtgta agtaaagaca atgtgtctga
     6841 atgcactgag tcctctggtg tcatagcaat aaggaaaaat gaattgactt tctgtgcata
     6901 cagtccagcc tgactggtgt atgatgttgc acttagcagc catgtggtgg gcatgtgtga
     6961 ctactctggg tttcacttta ttttctaaac tttttattcc tctcaagtcc agcatggatg
     7021 gggaaatgtc cctggagccc cgcagctgtg tacttgtttg catttgtttc ccattgagac
     7081 ttgtgtttgt gtcctgcttt gagctgtacc ctgtcccgtc cattgtgaaa ttatcccagc
     7141 agctgtaatg tacagttccc ttctcaagca agcaacagca gcacaatctt gtgttttata
     7201 aaaacaacag tggcttttat ttctaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaa
//
