LOCUS       NM_001081326            9625 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus amylo-1,6-glucosidase, 4-alpha-glucanotransferase
            (Agl), transcript variant 1, mRNA.
ACCESSION   NM_001081326 XM_894172
VERSION     NM_001081326.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 9625)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 9625)
  AUTHORS   Gardin,A., Rouillon,J., Montalvo-Romeral,V., Rossiaud,L., Vidal,P.,
            Launay,R., Vie,M., Krimi Benchekroun,Y., Cosette,J., Bertin,B., La
            Bella,T., Dubreuil,G., Nozi,J., Jauze,L., Fragnoud,R., Daniele,N.,
            Van Wittenberghe,L., Esque,J., Andre,I., Nissan,X., Hoch,L. and
            Ronzitti,G.
  TITLE     A functional mini-GDE transgene corrects impairment in models of
            glycogen storage disease type III
  JOURNAL   J Clin Invest 134 (2), e172018 (2024)
   PUBMED   38015640
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 9625)
  AUTHORS   Sun,R.C., Young,L.E.A., Bruntz,R.C., Markussen,K.H., Zhou,Z.,
            Conroy,L.R., Hawkinson,T.R., Clarke,H.A., Stanback,A.E.,
            Macedo,J.K.A., Emanuelle,S., Brewer,M.K., Rondon,A.L., Mestas,A.,
            Sanders,W.C., Mahalingan,K.K., Tang,B., Chikwana,V.M.,
            Segvich,D.M., Contreras,C.J., Allenger,E.J., Brainson,C.F.,
            Johnson,L.A., Taylor,R.E., Armstrong,D.D., Shaffer,R.,
            Waechter,C.J., Vander Kooi,C.W., DePaoli-Roach,A.A., Roach,P.J.,
            Hurley,T.D., Drake,R.R. and Gentry,M.S.
  TITLE     Brain glycogen serves as a critical glucosamine cache required for
            protein glycosylation
  JOURNAL   Cell Metab 33 (7), 1404-1417 (2021)
   PUBMED   34043942
REFERENCE   4  (bases 1 to 9625)
  AUTHORS   Roberts,G.A.G. and Tunster,S.J.
  TITLE     Characterising the dynamics of placental glycogen stores in the
            mouse
  JOURNAL   Placenta 99, 131-140 (2020)
   PUBMED   32798765
REFERENCE   5  (bases 1 to 9625)
  AUTHORS   Lim,J.A., Choi,S.J., Gao,F., Kishnani,P.S. and Sun,B.
  TITLE     A Novel Gene Therapy Approach for GSD III Using an AAV Vector
            Encoding a Bacterial Glycogen Debranching Enzyme
  JOURNAL   Mol Ther Methods Clin Dev 18, 240-249 (2020)
   PUBMED   32637453
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 9625)
  AUTHORS   Tamplin,O.J., Kinzel,D., Cox,B.J., Bell,C.E., Rossant,J. and
            Lickert,H.
  TITLE     Microarray analysis of Foxa2 mutant mouse embryos reveals novel
            gene expression and inductive roles for the gastrula organizer and
            its derivatives
  JOURNAL   BMC Genomics 9, 511 (2008)
   PUBMED   18973680
  REMARK    Publication Status: Online-Only
REFERENCE   7  (bases 1 to 9625)
  AUTHORS   Liu,S.P., Li,Y.S., Chen,Y.J., Chiang,E.P., Li,A.F., Lee,Y.H.,
            Tsai,T.F., Hsiao,M., Huang,S.F. and Chen,Y.M.
  TITLE     Glycine N-methyltransferase-/- mice develop chronic hepatitis and
            glycogen storage disease in the liver
  JOURNAL   Hepatology 46 (5), 1413-1425 (2007)
   PUBMED   17937387
  REMARK    Erratum:[Hepatology. 2008 Feb;47(2):769. Hwang, Shiu-Feng
            [corrected to Huang, Shiu-Feng]]
REFERENCE   8  (bases 1 to 9625)
  AUTHORS   Cheng,A., Zhang,M., Gentry,M.S., Worby,C.A., Dixon,J.E. and
            Saltiel,A.R.
  TITLE     A role for AGL ubiquitination in the glycogen storage disorders of
            Lafora and Cori's disease
  JOURNAL   Genes Dev 21 (19), 2399-2409 (2007)
   PUBMED   17908927
REFERENCE   9  (bases 1 to 9625)
  AUTHORS   Sakoda,H., Fujishiro,M., Fujio,J., Shojima,N., Ogihara,T.,
            Kushiyama,A., Fukushima,Y., Anai,M., Ono,H., Kikuchi,M., Horike,N.,
            Viana,A.Y., Uchijima,Y., Kurihara,H. and Asano,T.
  TITLE     Glycogen debranching enzyme association with beta-subunit regulates
            AMP-activated protein kinase activity
  JOURNAL   Am J Physiol Endocrinol Metab 289 (3), E474-E481 (2005)
   PUBMED   15886229
REFERENCE   10 (bases 1 to 9625)
  AUTHORS   Schliselfeld,L.H. and Danon,M.J.
  TITLE     Inverse relationship of skeletal muscle glycogen from wild-type and
            genetically modified mice to their phosphorylase a activity
  JOURNAL   Biochem Biophys Res Commun 290 (2), 874-877 (2002)
   PUBMED   11785984
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC147242.4.
            
            On Feb 6, 2007 this sequence version replaced XM_894172.2.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMN01164135
                              [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            inferred exon combination :: based on alignments, homology
            RefSeq Select criteria    :: based on conservation, expression,
                                         longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-139               AC147242.4         29389-29527
            140-289             AC147242.4         30138-30287
            290-500             AC147242.4         43733-43943
            501-667             AC147242.4         45371-45537
            668-871             AC147242.4         46346-46549
            872-1053            AC147242.4         48848-49029
            1054-1165           AC147242.4         49173-49284
            1166-1289           AC147242.4         50202-50325
            1290-1392           AC147242.4         50734-50836
            1393-1490           AC147242.4         50928-51025
            1491-1630           AC147242.4         52356-52495
            1631-1818           AC147242.4         52915-53102
            1819-1942           AC147242.4         54912-55035
            1943-2106           AC147242.4         55804-55967
            2107-2208           AC147242.4         56211-56312
            2209-2364           AC147242.4         56408-56563
            2365-2515           AC147242.4         56679-56829
            2516-2640           AC147242.4         58175-58299
            2641-2753           AC147242.4         58388-58500
            2754-2888           AC147242.4         58802-58936
            2889-3019           AC147242.4         60896-61026
            3020-3156           AC147242.4         64655-64791
            3157-3290           AC147242.4         65035-65168
            3291-3466           AC147242.4         66040-66215
            3467-3569           AC147242.4         68667-68769
            3570-3795           AC147242.4         78639-78864
            3796-3907           AC147242.4         79205-79316
            3908-4043           AC147242.4         82547-82682
            4044-4156           AC147242.4         84192-84304
            4157-4368           AC147242.4         85359-85570
            4369-4466           AC147242.4         87060-87157
            4467-4554           AC147242.4         90851-90938
            4555-4688           AC147242.4         91042-91175
            4689-9625           AC147242.4         92620-97556
FEATURES             Location/Qualifiers
     source          1..9625
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10090"
                     /chromosome="3"
                     /map="3 50.47 cM"
     gene            1..9625
                     /gene="Agl"
                     /gene_synonym="1110061O17Rik; 9430004C13Rik;
                     9630046L06Rik"
                     /note="amylo-1,6-glucosidase, 4-alpha-glucanotransferase"
                     /db_xref="GeneID:77559"
                     /db_xref="MGI:MGI:1924809"
     exon            1..139
                     /gene="Agl"
                     /gene_synonym="1110061O17Rik; 9430004C13Rik;
                     9630046L06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            140..289
                     /gene="Agl"
                     /gene_synonym="1110061O17Rik; 9430004C13Rik;
                     9630046L06Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             208..4806
                     /gene="Agl"
                     /gene_synonym="1110061O17Rik; 9430004C13Rik;
                     9630046L06Rik"
                     /EC_number="3.2.1.33"
                     /note="isoform 1 is encoded by transcript variant 1"
                     /codon_start=1
                     /product="glycogen debranching enzyme isoform 1"
                     /protein_id="NP_001074795.1"
                     /db_xref="CCDS:CCDS38613.1"
                     /db_xref="GeneID:77559"
                     /db_xref="MGI:MGI:1924809"
                     /translation="MEHSKQIRILLLNEMEKLEKTLFRLEQGFELQFRLGPTLQGKAV
                     TVYTNYPLPGEAFNREKFRSLDWENPTEREDDSDKYCKLHLQQSGSFQYYFLQGNEKS
                     GGGYIVVDPILRIGVDNHVLPLDCVTLQTFLAKCLGPFDEWESRLRVAKESGYNMIHF
                     TPLQTLGLSRSCYSLADQLELNPDFSRPSKRYTWSDVGQLVEKLKREWNILCITDVVY
                     NHTATNSKWILEHPESAYNLVNSPHLKPAWVLDRALWHFSCDVADGKYREKGVPALIE
                     NDQHMNCIRKIIWEDIFPRIQLWEFFQVDVHKAVEQFRRLLSQENRRVTKSEPKEHLK
                     IIQDPEYRRRGCAVDMDTALATFIPHDNGPAAIEECCNWFRKRLEELNSEKHHLTSCH
                     QEQAVNCLLGNVFYERLAGHGPKLGPVTRKYPLVTRYFTFPFGEMALSAEEALIHLPD
                     KACFLMAHNGWVMGDDPLRNFAEPGSDVYLRRELICWGDSVKLRYGNKPEDCPYLWAH
                     MKKYTEITATHFQGVRLDNCHSTPLHVAEYMLDAARKLQPNLYVVAELFTGSEELDNI
                     FVTRLGISSLIREAMSAYNSHEEGRLVYRYGGEPVGSFVQPCLRPLMPAIAHALFMDI
                     THDNECPIVHRSAYDALPSTTVVSMACCASGSTRGYDELVPHQISVVAEERFYTKWNP
                     GASPADTGDVNVHSGIIAARCAINRLHQELGAKGFIQVYVDQVDEDIVAVTRHSPSIH
                     QSVVAVSRTAFRNPKTSFYSKEVPQMCIPGKIEEVVLEARTIERNTKPYKKDENSING
                     MPNMTVELREHIQLHESKIVRQAGVATKGPNEYIQEIEFENLSPGSVIIFRVSLDPHA
                     QVAVGILRNHLTQFSSHFKSGSLAVDNADPILKIPFASIASKLTLAELNQVLYRCESE
                     EQEDGGGCYDIPNWSSLKYAGLQGLMSVLAEIRPKNDLGHPFCENLRSGDWMIDYVSG
                     RLISRSGSIAEVGKWLQAMFFYLKQIPRYLIPCYFDAILIGAYTTLLDVAWKQMSSFV
                     QNGSTFVKHLSLGSVQMCGVGKCPCLPLLSPSLLDVPCRLNEITKEKEQCCASLAAGL
                     PHFSSGLFRCWGRDTFIALRGMLLVTGRYLEARNIILAFASTLRHGLIPNLLGEGTYA
                     RYNCRDAVWWWLQCIQDYCRTVPNGLDILKCPVSRMYPTDDSAPLPAGTLDQPLFEVI
                     QEAMQRHMQGIQFRERNAGPQIDRNMKDEGFNITAGIDEETGFVYGGNRFNCGTWMDK
                     MGESDRARNRGIPATPRDGSAVEIVGLCKSAVRWLLELSKKNIFPYHEVRVKRHGKVV
                     AVSYDEWNRKIQNNFEKLFHVSEDPSDPNEKHPNLVHKRGIYKDSYGASSPWCDYQLR
                     PNFTIAMVVAPELFTAEKAWKALEIAEKKLLGPLGMKTLDPDDMVYCGVYDNALDNDN
                     YNLARGFNYHQGPEWLWPIGYFLRAKLYFSKMMGPETAAKTVFLVKNVLSRHYVHLER
                     SPWKGLPELTNENGQYCPFSCETQAWSMAVVLETLYDL"
     exon            290..500
                     /gene="Agl"
                     /gene_synonym="1110061O17Rik; 9430004C13Rik;
                     9630046L06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            501..667
                     /gene="Agl"
                     /gene_synonym="1110061O17Rik; 9430004C13Rik;
                     9630046L06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            668..871
                     /gene="Agl"
                     /gene_synonym="1110061O17Rik; 9430004C13Rik;
                     9630046L06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            872..1053
                     /gene="Agl"
                     /gene_synonym="1110061O17Rik; 9430004C13Rik;
                     9630046L06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1054..1165
                     /gene="Agl"
                     /gene_synonym="1110061O17Rik; 9430004C13Rik;
                     9630046L06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1166..1289
                     /gene="Agl"
                     /gene_synonym="1110061O17Rik; 9430004C13Rik;
                     9630046L06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1290..1392
                     /gene="Agl"
                     /gene_synonym="1110061O17Rik; 9430004C13Rik;
                     9630046L06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1393..1490
                     /gene="Agl"
                     /gene_synonym="1110061O17Rik; 9430004C13Rik;
                     9630046L06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1491..1630
                     /gene="Agl"
                     /gene_synonym="1110061O17Rik; 9430004C13Rik;
                     9630046L06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1631..1818
                     /gene="Agl"
                     /gene_synonym="1110061O17Rik; 9430004C13Rik;
                     9630046L06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1819..1942
                     /gene="Agl"
                     /gene_synonym="1110061O17Rik; 9430004C13Rik;
                     9630046L06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1943..2106
                     /gene="Agl"
                     /gene_synonym="1110061O17Rik; 9430004C13Rik;
                     9630046L06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2107..2208
                     /gene="Agl"
                     /gene_synonym="1110061O17Rik; 9430004C13Rik;
                     9630046L06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2209..2364
                     /gene="Agl"
                     /gene_synonym="1110061O17Rik; 9430004C13Rik;
                     9630046L06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2365..2515
                     /gene="Agl"
                     /gene_synonym="1110061O17Rik; 9430004C13Rik;
                     9630046L06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2516..2640
                     /gene="Agl"
                     /gene_synonym="1110061O17Rik; 9430004C13Rik;
                     9630046L06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2641..2753
                     /gene="Agl"
                     /gene_synonym="1110061O17Rik; 9430004C13Rik;
                     9630046L06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2754..2888
                     /gene="Agl"
                     /gene_synonym="1110061O17Rik; 9430004C13Rik;
                     9630046L06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2889..3019
                     /gene="Agl"
                     /gene_synonym="1110061O17Rik; 9430004C13Rik;
                     9630046L06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3020..3156
                     /gene="Agl"
                     /gene_synonym="1110061O17Rik; 9430004C13Rik;
                     9630046L06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3157..3290
                     /gene="Agl"
                     /gene_synonym="1110061O17Rik; 9430004C13Rik;
                     9630046L06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3291..3466
                     /gene="Agl"
                     /gene_synonym="1110061O17Rik; 9430004C13Rik;
                     9630046L06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3467..3569
                     /gene="Agl"
                     /gene_synonym="1110061O17Rik; 9430004C13Rik;
                     9630046L06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3570..3795
                     /gene="Agl"
                     /gene_synonym="1110061O17Rik; 9430004C13Rik;
                     9630046L06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3796..3907
                     /gene="Agl"
                     /gene_synonym="1110061O17Rik; 9430004C13Rik;
                     9630046L06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3908..4043
                     /gene="Agl"
                     /gene_synonym="1110061O17Rik; 9430004C13Rik;
                     9630046L06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            4044..4156
                     /gene="Agl"
                     /gene_synonym="1110061O17Rik; 9430004C13Rik;
                     9630046L06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            4157..4368
                     /gene="Agl"
                     /gene_synonym="1110061O17Rik; 9430004C13Rik;
                     9630046L06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            4369..4466
                     /gene="Agl"
                     /gene_synonym="1110061O17Rik; 9430004C13Rik;
                     9630046L06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            4467..4554
                     /gene="Agl"
                     /gene_synonym="1110061O17Rik; 9430004C13Rik;
                     9630046L06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            4555..4688
                     /gene="Agl"
                     /gene_synonym="1110061O17Rik; 9430004C13Rik;
                     9630046L06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            4689..9625
                     /gene="Agl"
                     /gene_synonym="1110061O17Rik; 9430004C13Rik;
                     9630046L06Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6553..6558
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Agl"
                     /gene_synonym="1110061O17Rik; 9430004C13Rik;
                     9630046L06Rik"
                     /note="hexamer: AATAAA"
     polyA_site      6574
                     /gene="Agl"
                     /gene_synonym="1110061O17Rik; 9430004C13Rik;
                     9630046L06Rik"
                     /note="major polyA site"
     regulatory      7093..7098
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Agl"
                     /gene_synonym="1110061O17Rik; 9430004C13Rik;
                     9630046L06Rik"
                     /note="hexamer: AATAAA"
     polyA_site      7118
                     /gene="Agl"
                     /gene_synonym="1110061O17Rik; 9430004C13Rik;
                     9630046L06Rik"
ORIGIN      
        1 tgtcccctgg cccgggtccc ggcgcccctc cccgtgccct tcacggccgc cctgccctcc
       61 ccgggccgcc gctgcgtccg gcgttcccgc tgtacccggc tccggctccc gctcccgacg
      121 ccgcacgtct atcccgctcg ggtaactcat tccactttgg agtcctttta gttctacgga
      181 aagagttcga atcctccaga agccaaaatg gaacacagta agcagattcg aattttacta
      241 ctgaacgaga tggagaagct agagaagacc ctcttcagac ttgaacaagg atttgaacta
      301 cagttccgat taggcccaac tttacaagga aaagctgtca ctgtatatac aaattaccca
      361 ttgcctggag aagcatttaa tcgagaaaag ttccgttcct tggattggga aaacccaacg
      421 gagagagaag acgattctga taaatactgt aaacttcatc tgcagcagtc tggatctttt
      481 cagtactact tccttcaagg aaatgagaaa agtggaggag gttacatagt cgtggatcct
      541 attttacgca ttggagttga taaccacgtg ttacccttgg actgtgttac tctgcagacg
      601 ttcttagcaa agtgcttagg acccttcgat gagtgggaaa gccggctaag ggtggcaaag
      661 gagtcaggtt acaacatgat tcacttcacc ccactgcaga ctcttggact gtcccggtca
      721 tgctattccc ttgccgatca gttagagcta aatcctgact tctcaaggcc tagtaaaaga
      781 tatacctgga gtgacgttgg gcagctagtg gagaagctga aaagggagtg gaatattctt
      841 tgtattaccg atgtggtcta caatcatact gccactaata gtaagtggat ccttgaacac
      901 ccggaatctg catacaatct tgtaaattct ccacacttga agcctgcctg ggtcctagac
      961 agagcactct ggcatttctc ctgtgatgtt gcagatggga agtacagaga aaaaggagtc
     1021 cctgctttga ttgaaaatga ccaacacatg aattgcattc gaaaaataat ttgggaggac
     1081 attttcccaa ggattcaact ctgggagttt ttccaagtag atgttcacaa agcagttgaa
     1141 caatttagaa gactgctctc tcaagaaaac agaagagtaa ctaagtctga accaaaagaa
     1201 catcttaaaa ttattcagga ccctgaatac aggcggcgag gctgcgctgt agatatggac
     1261 actgctctgg cgacttttat accacatgac aatgggccag ctgcaattga agagtgctgt
     1321 aactggttcc gtaagagact ggaggagtta aactcagaga agcaccacct caccagctgt
     1381 caccaggaac aggcagttaa ttgtcttttg ggaaacgtgt tttatgaacg actggcaggc
     1441 catggcccta aactaggacc tgtcaccagg aagtatcctt tagttaccag gtattttact
     1501 ttcccctttg gagaaatggc cttatccgca gaggaagccc tgatccacct cccagataaa
     1561 gcgtgcttcc tgatggcaca caacgggtgg gtgatgggcg atgacccgct ccggaacttt
     1621 gccgagccag gttcggatgt ttatttaagg agagaactta tttgctgggg tgacagtgtt
     1681 aaattgcgct atgggaataa acccgaggac tgcccttatc tgtgggcaca catgaagaaa
     1741 tacaccgaga taactgccac tcacttccag ggagttcgtc tggacaactg ccactccacg
     1801 cctctccacg tggctgagta catgctggat gctgctagga aattacagcc caatctttat
     1861 gtagtggctg agctgttcac aggaagcgaa gagctggaca atatcttcgt tactagactg
     1921 ggcatcagtt ccttaataag agaggcaatg agcgcgtata acagtcacga agagggcaga
     1981 ctggtctacc ggtacggagg ggaacctgtc ggctccttcg ttcagccctg tttgaggccg
     2041 ctcatgcctg ctatcgcgca tgccctgttt atggacatca cccacgataa tgagtgtccg
     2101 attgtgcaca gatcagccta tgatgctctt ccaagtacca cagttgtttc catggcatgc
     2161 tgtgccagtg gaagcacaag aggctacgac gagctagtgc ctcatcagat ttcagtggtt
     2221 gcagaggagc gattttacac taagtggaat cctggggcat caccggcaga tacaggggat
     2281 gttaatgtcc atagcgggat tattgcagcc agatgtgcca tcaataggct ccatcaagag
     2341 cttggagcca agggtttcat tcaagtgtat gtggatcaag tggatgaaga catagtggct
     2401 gtaaccaggc actccccgag catccaccag tctgttgtgg ctgtatcgag aactgctttt
     2461 aggaatccca agacttcctt ttacagcaaa gaagtgcctc agatgtgcat ccctggcaaa
     2521 attgaagagg tagttctgga agctagaact attgaaagga acacaaaacc ttataagaaa
     2581 gacgagaact cgatcaatgg aatgccgaac atgacagtgg aacttagaga acacatccag
     2641 cttcatgaaa gtaaaattgt cagacaagct ggagttgcca caaagggtcc caatgaatat
     2701 attcaggaaa tagaatttga gaacctatct ccaggaagtg ttattatatt cagagttagt
     2761 ctcgatccac acgctcaagt tgctgtgggg attcttcgga accacctgac ccagttcagc
     2821 tctcacttta agtccggaag ccttgctgtt gacaacgcag atcctatatt aaaaatcccc
     2881 ttcgcttcca ttgcctctaa attgactttg gctgagctaa accaggtact ttaccgatgt
     2941 gaatcagaag aacaagaaga cggtggcggt tgctatgaca taccaaactg gtcatcactt
     3001 aagtacgcag gacttcaagg attaatgtcc gtgttggcag aaataagacc gaagaatgac
     3061 ctggggcatc cattctgtga aaacttgagg tcgggagact ggatgatcga ctacgtgagt
     3121 ggtcggctta tttcaagatc aggaagtatt gctgaggttg gtaaatggct gcaggctatg
     3181 ttcttctacc tgaagcagat cccacgctac ctcatcccct gctactttga cgctatattg
     3241 attggtgcat acaccactct gctggatgta gcgtggaaac agatgtcaag ctttgttcag
     3301 aacggatcca ccttcgtgaa gcacctgtca ctgggttctg tccagatgtg tggagtcggg
     3361 aaatgccctt gtctgccgct gctttctccc tcgctcctgg atgtgccgtg caggttaaat
     3421 gagatcacaa aggagaagga gcagtgctgt gcctccttag ctgcaggctt acctcacttt
     3481 tcttccggtc tttttcgctg ctgggggagg gacactttca ttgcccttcg agggatgctg
     3541 ctggttactg gacgctacct ggaagccagg aacatcattt tagcgtttgc tagtaccctg
     3601 cggcacggtc tcatccctaa cctcctcggc gaagggacat atgccaggta caactgccgg
     3661 gacgccgtgt ggtggtggct gcagtgcatt caggactatt gcaggacagt tccaaacggc
     3721 ctggacatcc tcaaatgccc ggtgtccagg atgtatccaa cagatgactc cgcacccttg
     3781 cctgctggga cactggacca gcctttgttt gaagtcatcc aggaagctat gcagaggcac
     3841 atgcagggca tccagttccg agagaggaac gccggtccac agatcgaccg aaacatgaag
     3901 gacgagggtt tcaacataac tgcggggatt gatgaagaaa caggatttgt ttatggagga
     3961 aatcgcttca actgtggcac gtggatggat aaaatgggag agagtgaccg agctaggaac
     4021 agagggatcc cagccactcc aagagatggg tctgctgtgg aaatcgtggg cttgtgtaaa
     4081 tctgctgttc gttggttgct ggaattgtct aagaaaaata tctttcctta tcatgaagtc
     4141 agagtaaaaa gacatggaaa ggttgtggca gtctcgtacg atgagtggaa cagaaaaata
     4201 caaaacaatt tcgaaaagct gtttcatgtt tccgaagacc cttcagatcc taacgagaag
     4261 catccgaacc tggttcacaa acgcggcatc tacaaggaca gctatggtgc gtcgagtcct
     4321 tggtgtgact atcagctcag gcccaacttc accatagcaa tggtcgtggc ccctgaactc
     4381 tttactgctg agaaggcttg gaaggctttg gaaattgcag aaaaaaaatt gcttggtccc
     4441 cttggcatga aaactttaga cccagatgac atggtttact gtggagttta tgataatgcc
     4501 ttagacaatg acaactacaa ccttgctaga ggcttcaatt accaccaagg acctgagtgg
     4561 ctgtggccaa ttgggtattt tcttcgtgca aagttgtatt tttccaaaat gatgggtcca
     4621 gagactgctg cgaagactgt atttttggtt aaaaatgttc tttctagaca ttatgttcat
     4681 cttgagagat ccccatggaa aggccttccg gagctcacca atgagaacgg ccagtactgc
     4741 cctttcagct gtgagacaca ggcgtggtca atggctgttg ttctggaaac cctctatgat
     4801 ttatagttgg ctcataaata gtaattgtgt gaacacttga cattattgaa tgcaaggtca
     4861 taaagaaatg taagctcaac tcattttaaa aatctcattc atagccaggg aatggtggta
     4921 cacgccttta atcccagctc tcaggaggca aaggcaggca gatctcttga gttcaaggcc
     4981 agcctggtct acagggaaag ttcctggata gccagggcta cacagagaag cctgtgtcta
     5041 aaaaataaaa acaaaacaaa aaattattga tctctttttg gcttttttct tgaagactat
     5101 catgtgtgcc accgtaccca gtgtctgcag tgctggggat cacgcgggac atcatgcgta
     5161 ttagccgagc tctctaccaa ctgaacctca tcctgcgcct gtctttttct acattctgct
     5221 gatttgaatc agaaagaaat atgatcattt cccatagaag gttaccaaaa aagtattctt
     5281 caaagcaggg tttaaaacaa aaaaaggaaa gaaagaaaag cgtgtatcat cttcagtttg
     5341 gaggaagaga gaggtgatcc taagtcctgc tgtctgttgg cgcactccaa ggctgggctt
     5401 catggctgcc ctgccgtctg gtaacagcag tcctttttgt ttgtttgttt tgatttttcg
     5461 agacagggtt tctctgtata gccctggctg tcctcaaact cagaaatccg cctgcctctg
     5521 cctcccaagt gctgggatta aaggcgtgca ccaccatgcc cggctctaca gcagtcataa
     5581 aaccatgcct aatgcagtca gtacacactt ggtaattgct ttatgaatgt agaattatgg
     5641 aagcttccac ccacagtata tgttcataga tggagcacag agcttcaaaa tgtgctttat
     5701 ttttaacaga taagttatgt ttttaatata ataaatgcca actatactta acataaccta
     5761 tttgtgttaa agaataattg gacataataa aacaggccac caggacataa agcaattctg
     5821 ttaaattcca gtttcccact atgagatagt ttacattttc aaaatggaaa ttactgggca
     5881 tatttatgga atttttcaag gggaaaaaag cctagagtcg taccttgatg acagagtgtt
     5941 tgcttagcat gtgtcaagcc cttcgtacta tccctaaaac catcaagaga gaaagaaggg
     6001 agtgtgagag gggagggagg ggggaaagag aggaaagatt taaaatagcc atattaggtt
     6061 atctataatg gggtcaccat ccacctcaga aaatgggcag tgctcctact gagggctatc
     6121 aatgaaagac ctgtgctgtg ctgtgctttg cactgtctat ttgaaagtct cagaaggtat
     6181 ggtttacctt atcccagcca ctgtaattaa gtgaatgctt ttagactgtg aaaggatagt
     6241 tgccatttgg cctaagagca cttaggcaga gcgcttcatt cccgtgtgat gctgcatacc
     6301 gttgttttta tttacaatcc caaatgctct gtgccttggt ttttcactcg ccaaaaccaa
     6361 ccttactcta ctaaatgaaa tgcaatctta ccagtttaac cagtatgctg tgatattgta
     6421 gtgagtctca gagatgactg tggaaacagg aggcctgtca tttcaatgag caccatcacc
     6481 taagcggtaa tcatttctcc tgtgtcttaa actgctctga ctcctgagca agtgttcatg
     6541 tctgtgtgtc aaaataaaag ttttttctaa aggactctct ttgctttcct ccatggtttt
     6601 tactgtacat ttccctgtcg tcatgagtgg tgtgcagaga ctcacctttt attaaagtag
     6661 ctgtgtgaag taagctttcg gtgtatccct aagtctgtta gcatctactc ctttggtata
     6721 tcttgagtac ggtgaatctt tatttacgtt ttacttacca actcacaaat actgtagcaa
     6781 atggatgtga acatttactt tctgaaaaag ccagacaatt tttgttttca attatactac
     6841 tgagcaatct aagcatgtag aatatcttct aataaccaaa gctggtccat tctggttttg
     6901 ctttgcttac tgttgcttca atgtttttag agcagatgtt tttggttttt tggttttttt
     6961 gttttttcta atatatgtgg cttcattgtt ttaattggtg tgggtatcta acttacaaag
     7021 cttttacatt ttctttaact gggcctcatg tggtcccgag tagctcttgt aaacttagtc
     7081 ggacagtaag tcaataaact ctgcttgttt cctcctgagc ctatgtgtgc ccggtagcca
     7141 catctcgtac atctgcatca ggttcagtgg ttggtctttc ccccaggtta ccgtagtcca
     7201 gcgtcatgag ttgaatgtga tccgtggtga tatcttctac agacattcag gttttctttc
     7261 cttgttacct gcctctgttc acttgtttaa ctcttcttgt ctattcaccc ccaggctctg
     7321 cgccacccca actctattct ggggactaat cccgtggccc gctttaggct tgctgggaag
     7381 gtgcctagca ctgagctaac ccctcagctc ctgggtttgg ctcctttgtt tcagtgtttg
     7441 ctaaaccctt ttccaccctt ctcaccagtc cctacctttt gttttcaaag ccttgctcca
     7501 tacttgtagc tgtttgtttt tcttcagtgt ttttccaagt cccaaaattg ttagtacttt
     7561 taattaaatt gtgtggatgc tataaataaa tttgataagt accaacattt acttaaagtc
     7621 acacaatagt gaaacattgg gacaaaattc agcccctcat cttcaaatca gaaatcctct
     7681 ttggtagatc cttatacgtt cacaggtcca gctgtggctg gcgtcagctg cccctgattg
     7741 tgggaagcat tagatcctgt cctgaaggag tctgggtacc cacttagtgt cctctggtca
     7801 aaatgtttct agcctgtatt cctgggtaaa cattcagaat gacattgcca agcacaggct
     7861 aagttacacc actggagtac ctttgcaaat agaaatgtcc tatcaaagat gacaccggtg
     7921 atcaaagagt tgggactagg aattttagcc aggaattaaa tctcacgctc ggtgtgctaa
     7981 ttaaataact ggggggcggg gggcttgagg gggtgagcaa gtcctgtctg tggaagctga
     8041 ctagtaaata tggcacttaa ttctgccaat gttcaggtca agcaatttaa ggcagttagc
     8101 atattttgaa agtagggaac tgttgttttg ttttgagaca cagtctcact ttatagccca
     8161 gggttggcct ggaactcatt ttgtagtcta gtcggtcttc aaactcaagg cagtcctcct
     8221 gccttaacct tccaagtgct gggattatag acctaaaccg tagtgtccag atactgctca
     8281 gttttaatag atacactata gggaggaatg ctccaaaaaa gattcatctt gtaataacgt
     8341 gagcatagtt caggtcagcc ggcctggttg atctcagctc cttcactcag ggcgagggct
     8401 agctcagttc ctctgccctg gctgtgatag tactgggtag agcagcagtc ttcaacctgt
     8461 gggtcacatg tcagatgtct gcattatgat tcgtaacagt agcaacattg cagtcttgaa
     8521 gtagcaacaa aataactcgt ggtgtctgca tgaggaactg tgttaagggt cccggcgtta
     8581 ggaaggtatc tgttgaggat tgtgctttcc tctcgccctg atgttgtctt tgcttccctg
     8641 gctccccttt ctcgcctttc ctcctcttat gtctggcagc attttctcac tgaaggaact
     8701 gtcaacatga acctctctct ctcactcact ctcattctct ctctctctct ctctctctct
     8761 ctctctctct ctgtctctct gtctctctct ctgtctctgt cttgcacaca cacacacaaa
     8821 atgacaaact cttccccccc ccccatccaa aaagaaatct acctgtattt caacaataga
     8881 ttaatgctga aattttgact catacaaact aagggttttt tcttaaactc gtagattatt
     8941 aatttgaata acgtacagag aattttaagt ttgcttaaga tctctttgga taagaacacc
     9001 tattaaaaaa tatttgaggg ggctggtgag atggctcaga gggttagagc acccgactgt
     9061 tcttccgaag gtccagagtt caaatcccag caaccacatg gtggctcaca accatccgta
     9121 acgagatctg actccctctt ctggagtgtt tgaagacagc tacaatgtac ttacatataa
     9181 taaataaata aatctttaaa aaaaaatatt tgaggactag agttttgtca caaagataaa
     9241 actccaaccg ccttcacatt acttccttct ggacgtggaa gctgggtaaa caggagagtt
     9301 acttccttct tgtaaaatgt ttgtactgga gatgttgaaa ggccagctct gtgttctcag
     9361 gactgtaaat tatctaagca ttttgatgga tcgggctggc ttaatttcct ccctctagtt
     9421 aaaaagaaat gcagttgttt acatcttgct tgtagctaat cttaaaagag agccctgttt
     9481 cactcaggtc ttcagggcac gtgtgctaca gaattttttg gaaatgtgtg acttgcgcaa
     9541 agcttggtgg tagagcactt gcctagaatg tgtgaagaag tcctgtgtgt gtgtttaaaa
     9601 tgtacttttt aataaaactt tttaa
//
