LOCUS       NM_001033263            5645 bp    mRNA    linear   ROD 01-JUN-2024
DEFINITION  Mus musculus ArfGAP with GTPase domain, ankyrin repeat and PH
            domain 2 (Agap2), transcript variant 1, mRNA.
ACCESSION   NM_001033263 XM_618825 XM_898770 XM_906192 XM_919706
VERSION     NM_001033263.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5645)
  AUTHORS   Wilkinson,B., Li,J. and Coba,M.P.
  TITLE     Synaptic GAP and GEF Complexes Cluster Proteins Essential for GTP
            Signaling
  JOURNAL   Sci Rep 7 (1), 5272 (2017)
   PUBMED   28706196
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5645)
  AUTHORS   Tse,M.C.L., Herlea-Pana,O., Brobst,D., Yang,X., Wood,J., Hu,X.,
            Liu,Z., Lee,C.W., Zaw,A.M., Chow,B.K.C., Ye,K. and Chan,C.B.
  TITLE     Tumor Necrosis Factor-alpha Promotes Phosphoinositide 3-Kinase
            Enhancer A and AMP-Activated Protein Kinase Interaction to Suppress
            Lipid Oxidation in Skeletal Muscle
  JOURNAL   Diabetes 66 (7), 1858-1870 (2017)
   PUBMED   28404596
  REMARK    GeneRIF: Depletion of PIKE-A in C2C12 myotubes diminished the
            inhibitory activities of TNF-alpha on mitochondrial respiration and
            lipid oxidation, whereas PIKE-A overexpression exacerbated these
            cellular responses.
REFERENCE   3  (bases 1 to 5645)
  AUTHORS   Kang,S.S., Zhang,Z., Liu,X., Manfredsson,F.P., He,L., Iuvone,P.M.,
            Cao,X., Sun,Y.E., Jin,L. and Ye,K.
  TITLE     alpha-Synuclein binds and sequesters PIKE-L into Lewy bodies,
            triggering dopaminergic cell death via AMPK hyperactivation
  JOURNAL   Proc Natl Acad Sci U S A 114 (5), 1183-1188 (2017)
   PUBMED   28096359
  REMARK    GeneRIF: Dopamine and DOPAC were not changed in 3-mo-old mice but
            were decreased at 8 mo in the striatum of PIKE-KO mice compared
            with wild-type mice.  DA and DOPAC in hippocampus and substantia
            nigra were significantly decreased in 3-mo-old and 8-mo-old PIKE-KO
            mice as compared with wild-type mice. More severe motor defects in
            PIKE-KO and Fyn-KO mice than in wild-type mice exposed to alpha
            synuclein and MPTP.
REFERENCE   4  (bases 1 to 5645)
  AUTHORS   Ray,J.P., Staron,M.M., Shyer,J.A., Ho,P.C., Marshall,H.D.,
            Gray,S.M., Laidlaw,B.J., Araki,K., Ahmed,R., Kaech,S.M. and
            Craft,J.
  TITLE     The Interleukin-2-mTORc1 Kinase Axis Defines the Signaling,
            Differentiation, and Metabolism of T Helper 1 and Follicular B
            Helper T Cells
  JOURNAL   Immunity 43 (4), 690-702 (2015)
   PUBMED   26410627
REFERENCE   5  (bases 1 to 5645)
  AUTHORS   Chan,C.B., Liu,X., Zhao,L., Liu,G., Lee,C.W., Feng,Y. and Ye,K.
  TITLE     PIKE is essential for oligodendroglia development and CNS
            myelination
  JOURNAL   Proc Natl Acad Sci U S A 111 (5), 1993-1998 (2014)
   PUBMED   24449917
  REMARK    GeneRIF: PIKE plays pivotal roles to advance oligodendroglia
            development and myelinogenesis through Akt/mTOR activation.
REFERENCE   6  (bases 1 to 5645)
  AUTHORS   Chan,C.B., Liu,X., Jung,D.Y., Jun,J.Y., Luo,H.R., Kim,J.K. and
            Ye,K.
  TITLE     Deficiency of phosphoinositide 3-kinase enhancer protects mice from
            diet-induced obesity and insulin resistance
  JOURNAL   Diabetes 59 (4), 883-893 (2010)
   PUBMED   20068140
  REMARK    GeneRIF: PIKE knockout mice show augmented lipid oxidation, which
            is accompanied by enhanced AMPK phosphorylation in muscle and
            adipose tissue and is implicated in obesity and diabetes
            development.
REFERENCE   7  (bases 1 to 5645)
  AUTHORS   Tang,X., Jang,S.W., Okada,M., Chan,C.B., Feng,Y., Liu,Y., Luo,S.W.,
            Hong,Y., Rama,N., Xiong,W.C., Mehlen,P. and Ye,K.
  TITLE     Netrin-1 mediates neuronal survival through PIKE-L interaction with
            the dependence receptor UNC5B
  JOURNAL   Nat Cell Biol 10 (6), 698-706 (2008)
   PUBMED   18469807
  REMARK    GeneRIF: PIKE-L acts as a downstream survival effector for netrin-1
            through UNC5B in the nervous system
REFERENCE   8  (bases 1 to 5645)
  AUTHORS   Liu,Z., Jang,S.W., Liu,X., Cheng,D., Peng,J., Yepes,M., Li,X.J.,
            Matthews,S., Watts,C., Asano,M., Hara-Nishimura,I., Luo,H.R. and
            Ye,K.
  TITLE     Neuroprotective actions of PIKE-L by inhibition of SET proteolytic
            degradation by asparagine endopeptidase
  JOURNAL   Mol Cell 29 (6), 665-678 (2008)
   PUBMED   18374643
REFERENCE   9  (bases 1 to 5645)
  AUTHORS   Tang,X., Feng,Y. and Ye,K.
  TITLE     Src-family tyrosine kinase fyn phosphorylates phosphatidylinositol
            3-kinase enhancer-activating Akt, preventing its apoptotic cleavage
            and promoting cell survival
  JOURNAL   Cell Death Differ 14 (2), 368-377 (2007)
   PUBMED   16841086
REFERENCE   10 (bases 1 to 5645)
  AUTHORS   Ye,K., Hurt,K.J., Wu,F.Y., Fang,M., Luo,H.R., Hong,J.J.,
            Blackshaw,S., Ferris,C.D. and Snyder,S.H.
  TITLE     Pike. A nuclear gtpase that enhances PI3kinase activity and is
            regulated by protein 4.1N
  JOURNAL   Cell 103 (6), 919-930 (2000)
   PUBMED   11136977
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK147446.1, AC131760.4 and
            AA189612.1.
            
            On Jul 19, 2014 this sequence version replaced NM_001033263.4.
            
            Summary: The protein encoded by this gene is a member of the
            centaurin GTPase family. This gene product regulates the activity
            of multiple kinases, including PI3K. Reduced expression of this
            gene results in multiple defects, including neural deficiencies,
            while increased expression of this gene has been observed in some
            tumors. Alternative splicing results in multiple protein isoforms.
            [provided by RefSeq, Jul 2014].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK147446.1, SRR7652917.995628.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131, SAMN01164132
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-4571              AK147446.1         2-4572
            4572-4572           AC131760.4         70754-70754         c
            4573-5499           AK147446.1         4574-5500
            5500-5501           AC131760.4         69825-69826         c
            5502-5597           AK147446.1         5501-5596
            5598-5645           AA189612.1         433-480
FEATURES             Location/Qualifiers
     source          1..5645
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="10"
                     /map="10 74.5 cM"
     gene            1..5645
                     /gene="Agap2"
                     /gene_synonym="AGAP-2; Centg1; cnt-g1; mKIAA0167; PIKE"
                     /note="ArfGAP with GTPase domain, ankyrin repeat and PH
                     domain 2"
                     /db_xref="GeneID:216439"
                     /db_xref="MGI:MGI:3580016"
     exon            1..1865
                     /gene="Agap2"
                     /gene_synonym="AGAP-2; Centg1; cnt-g1; mKIAA0167; PIKE"
                     /inference="alignment:Splign:2.1.0"
     CDS             716..4276
                     /gene="Agap2"
                     /gene_synonym="AGAP-2; Centg1; cnt-g1; mKIAA0167; PIKE"
                     /note="isoform 1 is encoded by transcript variant 1;
                     centaurin, gamma 1; arf-GAP with GTPase, ANK repeat and PH
                     domain-containing protein 2; phosphatidylinositol 3-kinase
                     enhancer"
                     /codon_start=1
                     /product="arf-GAP with GTPase, ANK repeat and PH
                     domain-containing protein 2 isoform 1"
                     /protein_id="NP_001028435.1"
                     /db_xref="CCDS:CCDS24228.1"
                     /db_xref="GeneID:216439"
                     /db_xref="MGI:MGI:3580016"
                     /translation="MSRGAGALQRRTTTYLISLTLVKLESVPPPPPSPSAAAAGAPGA
                     RGSEPRDPGSPRGSEEPGKKRHERLFHRQDALWISTSSAGTGGAEPPALSPAPASPAR
                     PVSPAPGRRLSLWAAPPGPPLSGGLSPDSKPGGAPSSSRRPLLSSPSWGGPEPEGRTG
                     GGVPGSSSPHPGTGSRRLKVAPPPPAPKPFKTVTTSGAKAGGGKGAGSRLSWPESEGK
                     PRVKGSKSTAGTGASAAAAGGGGSAAVTTSGGVGAGAGTRGKLSPRKGKSKTLDNSDL
                     HPGPSAGSPPLTVPAIPVPATSVTATSTQPLGPAPPITLEPPAPGLKRGREGGRASTR
                     DRKMLKFISGIFTKSTGGPPGPGPLPGPQGLSSSSGSRELLGAELRTSPKAVVNSQEW
                     TLSRSIPELRLGVLGDVRSGKSSLIHRFLTGSYQVLEKIESEQYKKEMLVDGQTHLVL
                     IREEAGAPDAKFSGWADAVIFVFSLEDESSFQAVSRLHGQLSSLRGEGRGGLALALVG
                     TQDRISASSPRVVGDARARALCTDMKRCSYYETCATYGLNVDRVFQEVAQKVVTLRKQ
                     QQLLAACKSLPSSPSHSAASTPVAGQASNGGHTSDYSSSLPSSPNVGHRELRAEAAAV
                     AGLSTPGSLHRAAKRRTSLFANRRGSDAEKRSLDSRGETTGSGRAIPIKQSFLLKRSG
                     NSLNKEWKKKYVTLSSNGFLLYHPSINDYIHSTHGKEMDLLRTTVKVPGKRPPRAISA
                     FGPSASINGLVKDMSTVQMGEGPEASTPMPSPSPSPSSLQLPTDQTSKHLLKPDRNLA
                     RALSTDCTPSGDLSPLSREPPPSPMVKKQRRKKLSTPSKTEGSAVQAEAKRKMWKLKS
                     FGSLRNIYKAEENFEFLIVSSTGQTWHFEAASFEERDAWVQAIESQILASLQCCESSK
                     VKLRTDSQSEAVAIQAIRNAKGNSTCVDCGAPNPTWASLNLGALICIECSGIHRNLGT
                     HLSRVRSLDLDDWPRELTLVLTAIGNDTANRVWESDTRGRAKPTRDSSREERESWIRA
                     KYEQLLFLAPLGTTEEPLGRQLWAAVEAQDVAAVLLLLAHARHGPLDTSVEDPQLRSP
                     LHLAAELAHVVITQLLLWYGADVAARDAQGRTALFYARQAGSQLCADILLQHGCPGEG
                     GSTATTPSAATTPSITATPSPRRRSSAASLGRVDTTIALV"
     exon            1866..1924
                     /gene="Agap2"
                     /gene_synonym="AGAP-2; Centg1; cnt-g1; mKIAA0167; PIKE"
                     /inference="alignment:Splign:2.1.0"
     exon            1925..2012
                     /gene="Agap2"
                     /gene_synonym="AGAP-2; Centg1; cnt-g1; mKIAA0167; PIKE"
                     /inference="alignment:Splign:2.1.0"
     exon            2013..2098
                     /gene="Agap2"
                     /gene_synonym="AGAP-2; Centg1; cnt-g1; mKIAA0167; PIKE"
                     /inference="alignment:Splign:2.1.0"
     exon            2099..2246
                     /gene="Agap2"
                     /gene_synonym="AGAP-2; Centg1; cnt-g1; mKIAA0167; PIKE"
                     /inference="alignment:Splign:2.1.0"
     exon            2247..2381
                     /gene="Agap2"
                     /gene_synonym="AGAP-2; Centg1; cnt-g1; mKIAA0167; PIKE"
                     /inference="alignment:Splign:2.1.0"
     exon            2382..2491
                     /gene="Agap2"
                     /gene_synonym="AGAP-2; Centg1; cnt-g1; mKIAA0167; PIKE"
                     /inference="alignment:Splign:2.1.0"
     exon            2492..2650
                     /gene="Agap2"
                     /gene_synonym="AGAP-2; Centg1; cnt-g1; mKIAA0167; PIKE"
                     /inference="alignment:Splign:2.1.0"
     exon            2651..2737
                     /gene="Agap2"
                     /gene_synonym="AGAP-2; Centg1; cnt-g1; mKIAA0167; PIKE"
                     /inference="alignment:Splign:2.1.0"
     exon            2738..2842
                     /gene="Agap2"
                     /gene_synonym="AGAP-2; Centg1; cnt-g1; mKIAA0167; PIKE"
                     /inference="alignment:Splign:2.1.0"
     exon            2843..3005
                     /gene="Agap2"
                     /gene_synonym="AGAP-2; Centg1; cnt-g1; mKIAA0167; PIKE"
                     /inference="alignment:Splign:2.1.0"
     exon            3006..3125
                     /gene="Agap2"
                     /gene_synonym="AGAP-2; Centg1; cnt-g1; mKIAA0167; PIKE"
                     /inference="alignment:Splign:2.1.0"
     exon            3126..3254
                     /gene="Agap2"
                     /gene_synonym="AGAP-2; Centg1; cnt-g1; mKIAA0167; PIKE"
                     /inference="alignment:Splign:2.1.0"
     exon            3255..3314
                     /gene="Agap2"
                     /gene_synonym="AGAP-2; Centg1; cnt-g1; mKIAA0167; PIKE"
                     /inference="alignment:Splign:2.1.0"
     exon            3315..3463
                     /gene="Agap2"
                     /gene_synonym="AGAP-2; Centg1; cnt-g1; mKIAA0167; PIKE"
                     /inference="alignment:Splign:2.1.0"
     exon            3464..3554
                     /gene="Agap2"
                     /gene_synonym="AGAP-2; Centg1; cnt-g1; mKIAA0167; PIKE"
                     /inference="alignment:Splign:2.1.0"
     exon            3555..3777
                     /gene="Agap2"
                     /gene_synonym="AGAP-2; Centg1; cnt-g1; mKIAA0167; PIKE"
                     /inference="alignment:Splign:2.1.0"
     exon            3778..4033
                     /gene="Agap2"
                     /gene_synonym="AGAP-2; Centg1; cnt-g1; mKIAA0167; PIKE"
                     /inference="alignment:Splign:2.1.0"
     exon            4034..5634
                     /gene="Agap2"
                     /gene_synonym="AGAP-2; Centg1; cnt-g1; mKIAA0167; PIKE"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5610..5615
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Agap2"
                     /gene_synonym="AGAP-2; Centg1; cnt-g1; mKIAA0167; PIKE"
                     /note="hexamer: ATTAAA"
     polyA_site      5634
                     /gene="Agap2"
                     /gene_synonym="AGAP-2; Centg1; cnt-g1; mKIAA0167; PIKE"
                     /note="major polyA site"
ORIGIN      
        1 ctcaatgtga ggggagcgcc cggacacaac gcgcatccgg accgagccga tcccgggcga
       61 accccccccc ctactcttct ccaggatcca cgcccctccc ctcccagacc ccagacccgt
      121 gggttcctat ctcctcccgt tccctctttc ctccctcacc gcctccaata gctccatccc
      181 tagcatcctc cccgtgctcc tgcgaatccc ccgcacagac agccccacta gtctctaccc
      241 tcaagacccc cctttcccac tgcatctgcg gcctctgccc attgagtccg cattcccgcc
      301 cctgccttcc cctcacccaa gcttctcccc caggcccctg tccccctttc taccctcttt
      361 cccttcatcc ccatttgccc ccactactgt cctctccacc ctcctccgcc catctcaacg
      421 cccccaccca gaccggggtg cacgatccgg cgcgcctccc tggagcagca ccaaggtccc
      481 ggggctccaa gggggtgggg gcgcagggcg gggagcatgg ggagggggcg ccccatgtga
      541 tgggaggggg gcgcaagagg aggcggcgga ggcggcggcg acagcggagc cgggccgggg
      601 gctcagaggg gtccgggggc tgggggcagc gagcagggcg gccccatggc cgggcccccc
      661 tgaggcatac ccggggagtc ccctcccctc cccagctcgg gggtctccgg gccccatgag
      721 ccggggcgcg ggcgcgcttc agcgccggac aacgacctac ctcatctcgc tgaccctggt
      781 caagctcgag tcggtacctc cgccgccgcc ttctccatct gcagccgcag ccggcgcccc
      841 cggggccaga ggctccgagc cccgagatcc tggcagcccc cgaggctcgg aggaacccgg
      901 caagaagcgg cacgagcgtc tcttccaccg gcaggatgcg ctgtggatca gtaccagcag
      961 cgcaggcacc gggggtgcgg agccccccgc gctgtccccg gctccggcca gtccggctcg
     1021 cccggtctcc ccggctccgg gccgccgact gtccctatgg gccgcccctc cgggaccccc
     1081 gctctccggg gggctgagcc cggactccaa gccgggaggc gccccctctt cctcccggcg
     1141 ccctctactc agcagcccga gctggggggg tccggaacct gaaggccgga cgggtggcgg
     1201 agtcccgggc tcgtcctccc cgcatcctgg caccggtagc cgaaggctca aggtggcgcc
     1261 tcctccacct gctcccaagc ctttcaagac cgtgaccact agtggagcca aagccggcgg
     1321 gggcaagggc gcaggcagcc gcctgtcatg gcccgaaagc gagggcaaac ccagggtcaa
     1381 ggggtcaaag agcaccgccg ggactggagc ttctgccgct gcggccggcg ggggagggag
     1441 cgctgcagtc acgacctctg gtggggtcgg ggctggggct ggaaccagag ggaagttgtc
     1501 ccctcggaaa ggcaagagta agaccttgga caatagtgac ttacacccag gacccagtgc
     1561 cggctctcct ccgctcaccg tgccagcaat cccagttcca gccacttctg tcactgccac
     1621 ctccacgcag cccctcgggc ctgcaccccc tatcactctg gagccaccag ctccagggct
     1681 gaaaaggggc cgggagggcg gccgagcgtc cactcgtgac cgcaagatgc tcaagtttat
     1741 cagcggtatc ttcaccaaga gcacaggagg gcctcctggt ccagggcccc ttcccggacc
     1801 ccaaggcttg tcttccagca gcgggtccag ggagctgctg ggagcagaac tacgcacctc
     1861 ccctaaggct gtggttaata gccaggaatg gactttgagc cgatcaattc ccgaactgcg
     1921 cctgggtgtg ctgggtgatg tcaggagtgg gaagtcgtcc ctcatccata gattcctcac
     1981 gggttcatac caggtgctgg agaagattga gagtgagcag tacaagaagg agatgctagt
     2041 ggatgggcag acccatctgg tgctgatccg ggaggaagca ggggcacccg atgccaagtt
     2101 ctcaggctgg gcagatgctg tgatcttcgt cttcagcctg gaggatgaga gcagcttcca
     2161 ggctgtgagc cgtctccatg ggcagctgag ctccctccgg ggggaggggc gaggaggtct
     2221 ggccctggct ctggttggga cccaagacag gatcagtgca tcatctcctc gagtggtggg
     2281 tgatgctcga gccagggctc tgtgcacgga catgaagcgc tgtagctact acgagacctg
     2341 cgcaacctat gggctcaatg tggaccgggt cttccaggag gtggcccaga aggtggtgac
     2401 cttgcgcaaa caacaacaac tcctggctgc ttgcaaatcc ctgcctagct cgccaagcca
     2461 ttcagctgca tctactcctg tagctgggca ggctagtaac gggggccaca ctagcgacta
     2521 ctcttcttcc ctcccatcct cacccaacgt tggacaccgg gagctccgag ctgaggcagc
     2581 tgcggtggct gggttgagta ccccagggtc cttacacaga gcagccaagc ggcggaccag
     2641 cctttttgcg aaccgtcggg gcagcgatgc tgagaagcgg agcttagaca gccggggaga
     2701 gacaacaggg agtgggcgag ccatccccat caaacagagc ttcctgctaa aacgaagtgg
     2761 caattccttg aacaaagaat ggaagaagaa atatgtgacc ttgtccagca atggctttct
     2821 actctaccac cccagcatta atgactacat ccacagtaca catggcaaag agatggactt
     2881 gttacgaaca acggtcaaag tcccaggcaa gcggccccca agggctatct ctgctttcgg
     2941 cccctcagcc agcatcaacg ggttggtcaa ggatatgagc accgtccaga tgggtgaagg
     3001 ccctgaagcc tctactccca tgccaagccc cagccctagc cccagttccc tgcagcttcc
     3061 aacagaccag acatccaagc acctcttgaa gccagaccgg aatttggccc gtgcccttag
     3121 caccgactgt accccatctg gagatctgag tcccctgagt cgggaacccc caccttcgcc
     3181 catggtgaag aagcagagga ggaagaaact gtccacgcct tctaagactg aaggctcggc
     3241 tgtgcaagct gaagccaagc gcaaaatgtg gaaactaaaa tcctttggta gtttaagaaa
     3301 tatttataaa gcagaggaaa actttgagtt cctgatcgtg tccagcactg gtcagacgtg
     3361 gcactttgag gcagccagct ttgaggagcg ggatgcttgg gttcaggcca ttgagagtca
     3421 gatcctggcc agtctgcagt gctgtgagag cagcaaggtc aagctgcgca ctgacagcca
     3481 aagcgaagct gtggccatcc aggcgatccg caacgcaaag ggaaactcga cctgtgtgga
     3541 ctgtggagct cccaacccca cgtgggccag tttgaatctg ggcgcactca tctgcatcga
     3601 gtgttctggc atccaccgga acctgggcac acacctgtcc cgagtgcgtt cactggattt
     3661 ggatgactgg ccgcgggagc tgaccctggt gctgacggcc attggcaacg acacagccaa
     3721 ccgcgtgtgg gagagcgaca ctcgaggccg tgccaagcct acacgggact cttcgcggga
     3781 ggagcgtgag tcgtggattc gcgccaagta tgagcagctg ctgttcctgg cgcctctggg
     3841 caccacggag gagccgctgg gccgccaact gtgggccgct gtggaggccc aggacgtggc
     3901 cgccgttctg ctgcttctgg cccacgcgcg acatgggccg ctcgacacca gcgtggagga
     3961 cccgcagctc cgctcgccgc tgcacttggc ggccgagctc gcccacgttg tcattacaca
     4021 gctgctgttg tggtatggcg cagacgtggc ggcccgcgat gcgcaaggcc gcacggcact
     4081 gttctatgcc cgccaggctg gcagccagct gtgtgcagac atccttctcc agcacggctg
     4141 cccgggagag ggcggcagca cggccaccac gcccagcgcg gccaccaccc ccagcattac
     4201 cgccacgccc agcccgaggc gccggagcag tgctgccagc ttgggccgcg tggacaccac
     4261 gatcgcgctg gtatagttgc ccagcagcgg gagagacacc ccccaccccc gcgcgggccg
     4321 ggcacgacta caccgggtgg accgttggac agatgcaccc actcacctct ccagtccgca
     4381 ccccgcccca cgggagcact ttctaccccc acgagggcac agccccgcac gcctcccagg
     4441 agacacaaac tcacctccct ctccccagtg aggcatggag accctagctc atcacgcccc
     4501 tacttcacag ctttcttact tggattgccc tgggtctacc tgctgggtgt ttgtaggtgg
     4561 gcactccctt gcagctgttc cgggctccaa ggtgtttgga cttgctggct tgtgcccccc
     4621 ctaggggcgg ggaaaagatc caatcctgcc tgcgttctac aatcgggtgg cgggagccga
     4681 atcccaggct ggctcctggg gcaactccac gccagaggga ggggttagta cgtggaagac
     4741 tagccctgag atttgggacc aacttgggga ccccacccct tccatgctaa tctcactgcc
     4801 cagtgagggg ggagggcagc gaggggcagg acccctgctg cccatggagg tggaggggtc
     4861 cccaaccctt tccgtgaaag ggaccatgag gagtggaaat cagatatccc ccctctacct
     4921 atcttatgaa tggaagctaa agagccaggg ggagccgaga gcctgggtcc ttccccttac
     4981 gatcatgggg tagagggaga aggattggaa caggaggtga ctagagaggg gccttgtaat
     5041 ttattgcttt gttccccaac atggggatgg ggtgggtagg ggcgagggga gggcatatca
     5101 gggtagggga agccaggggt gggcaggggt ggggtagggt gggggtgctc tcgggttgtg
     5161 tctgtgaccg tgactgcgta cctgtgactg tgcagcgccc gtggtgatct ggggtagggg
     5221 gcacccctac aacgggaccc ctcccccata atcctttctg tccagcccct ctctccctgg
     5281 agcagctcca ggtccgtttt ttctatcctt tgctccctcc cagccagggc tgagagggtg
     5341 tgtagtggtt gggaggactt taaggtcctg gcccccatcc ccagaaaggg agaggtggtg
     5401 tggacttatc tggcccagcc ctgccaccct tagtcagccc ggctttgggc tgtctgtgtc
     5461 agtggtttcc ggtctttttt tttttttttt tttttttttt tcctggctaa aatattttgc
     5521 aaaagaccag gtaattgggg agtggagtga agagggggca ggggttaccc ccctcacctc
     5581 caaagcaggt ggtgtgaatc ttaacagcaa ttaaagagca agtgattttg tctaaaaaaa
     5641 aaaaa
//
