LOCUS       NM_178119               9581 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus ArfGAP with GTPase domain, ankyrin repeat and PH
            domain 1 (Agap1), transcript variant 1, mRNA.
ACCESSION   NM_178119 XM_907301 XM_920295 XM_920309
VERSION     NM_178119.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 9581)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 9581)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 9581)
  AUTHORS   Ingham,N.J., Pearson,S.A., Vancollie,V.E., Rook,V., Lewis,M.A.,
            Chen,J., Buniello,A., Martelletti,E., Preite,L., Lam,C.C.,
            Weiss,F.D., Powis,Z., Suwannarat,P., Lelliott,C.J., Dawson,S.J.,
            White,J.K. and Steel,K.P.
  TITLE     Mouse screen reveals multiple new genes underlying mouse and human
            hearing loss
  JOURNAL   PLoS Biol 17 (4), e3000194 (2019)
   PUBMED   30973865
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 9581)
  AUTHORS   Arnold,M., Cross,R., Singleton,K.S., Zlatic,S., Chapleau,C.,
            Mullin,A.P., Rolle,I., Moore,C.C., Theibert,A., Pozzo-Miller,L.,
            Faundez,V. and Larimore,J.
  TITLE     The Endosome Localized Arf-GAP AGAP1 Modulates Dendritic Spine
            Morphology Downstream of the Neurodevelopmental Disorder Factor
            Dysbindin
  JOURNAL   Front Cell Neurosci 10, 218 (2016)
   PUBMED   27713690
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 9581)
  AUTHORS   Luo,R., Akpan,I.O., Hayashi,R., Sramko,M., Barr,V., Shiba,Y. and
            Randazzo,P.A.
  TITLE     GTP-binding protein-like domain of AGAP1 is protein binding site
            that allosterically regulates ArfGAP protein catalytic activity
  JOURNAL   J Biol Chem 287 (21), 17176-17185 (2012)
   PUBMED   22453919
  REMARK    GeneRIF: GTP-binding protein-like domain of AGAP1 is protein
            binding site that allosterically regulates ArfGAP protein catalytic
            activity.
REFERENCE   6  (bases 1 to 9581)
  AUTHORS   Taniwaki,T., Haruna,K., Nakamura,H., Sekimoto,T., Oike,Y.,
            Imaizumi,T., Saito,F., Muta,M., Soejima,Y., Utoh,A., Nakagata,N.,
            Araki,M., Yamamura,K. and Araki,K.
  TITLE     Characterization of an exchangeable gene trap using pU-17 carrying
            a stop codon-beta geo cassette
  JOURNAL   Dev Growth Differ 47 (3), 163-172 (2005)
   PUBMED   15840001
REFERENCE   7  (bases 1 to 9581)
  AUTHORS   Meurer,S., Pioch,S., Wagner,K., Muller-Esterl,W. and Gross,S.
  TITLE     AGAP1, a novel binding partner of nitric oxide-sensitive guanylyl
            cyclase
  JOURNAL   J Biol Chem 279 (47), 49346-49354 (2004)
   PUBMED   15381706
  REMARK    GeneRIF: AGAP1 is a novel binding partner of nitric oxide-sensitive
            guanylyl cyclase
REFERENCE   8  (bases 1 to 9581)
  AUTHORS   Xia,C., Ma,W., Stafford,L.J., Liu,C., Gong,L., Martin,J.F. and
            Liu,M.
  TITLE     GGAPs, a new family of bifunctional GTP-binding and
            GTPase-activating proteins
  JOURNAL   Mol Cell Biol 23 (7), 2476-2488 (2003)
   PUBMED   12640130
REFERENCE   9  (bases 1 to 9581)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   10 (bases 1 to 9581)
  AUTHORS   Araki,K., Imaizumi,T., Sekimoto,T., Yoshinobu,K., Yoshimuta,J.,
            Akizuki,M., Miura,K., Araki,M. and Yamamura,K.
  TITLE     Exchangeable gene trap using the Cre/mutated lox system
  JOURNAL   Cell Mol Biol (Noisy-le-grand) 45 (5), 737-750 (1999)
   PUBMED   10512203
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK147503.1, AK046523.1 and AC124227.21.
            
            [WARNING] On Jan 30, 2025 this sequence was replaced by
            NM_178119.4.
            
            On or before Dec 7, 2005 this sequence version replaced
            XM_920295.1, XM_920309.1, XM_907301.1, NM_178119.2.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and it encodes the longer protein (isoform 1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK147503.1, AK046523.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-554               AK147503.1         7-560
            555-4198            AK046523.1         516-4159
            4199-9581           AC124227.21        155489-160871       c
FEATURES             Location/Qualifiers
     source          1..9581
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 45.06 cM"
     gene            1..9581
                     /gene="Agap1"
                     /gene_synonym="Centg2; Ggap1; mKIAA1099"
                     /note="ArfGAP with GTPase domain, ankyrin repeat and PH
                     domain 1"
                     /db_xref="GeneID:347722"
                     /db_xref="MGI:MGI:2653690"
     exon            1..782
                     /gene="Agap1"
                     /gene_synonym="Centg2; Ggap1; mKIAA1099"
                     /inference="alignment:Splign:2.1.0"
     CDS             620..3193
                     /gene="Agap1"
                     /gene_synonym="Centg2; Ggap1; mKIAA1099"
                     /EC_number="3.6.5.2"
                     /note="isoform 1 is encoded by transcript variant 1;
                     arf-GAP with GTPase, ANK repeat and PH domain-containing
                     protein 1; AGAP-1; cnt-g2; centaurin-gamma-2"
                     /codon_start=1
                     /product="arf-GAP with GTPase, ANK repeat and PH
                     domain-containing protein 1 isoform 1"
                     /protein_id="NP_835220.1"
                     /db_xref="CCDS:CCDS15149.1"
                     /db_xref="GeneID:347722"
                     /db_xref="MGI:MGI:2653690"
                     /translation="MNYQQQLANSAAIRAEIQRFESVHPNIYSIYELLERVEEPVLQN
                     QIREHVIAIEDAFVNSQEWTLSRSVPELKVGIVGNLASGKSALVHRYLTGTYVQEESP
                     EGGRFKKEIVVDGQSYLLLIRDEGGPPEAQFAMWVDAVIFVFSLEDEISFQTVYHYYS
                     RMANYRNTSEIPLVLVGTQDAISSTNPRVIDDVRARKLSNDLKRCTYYETCATYGLNV
                     ERVFQDVAQKIVATRKKQQLSIGPCKSLPNSPSHSSVCSAQVSAVHISQTSNGGGSLS
                     DYSSSVPSTPSTSQKELRIDVPPTANTPTPVRKQSKRRSNLFTSRKGSDPDKEKKGLE
                     SRADSIGSGRAIPIKQGMLLKRSGKSLNKEWKKKYVTLCDNGVLTYHPSLHDYMQNVH
                     GKEIDLLRTTVKVPGKRPPRATSACAPISSPKTNGLAKDMSSLHISPNSGNVTSASGS
                     QMASGISLVSFNSRPDGMHQRSYSVSSADQWSDATVIANSAISSDTGLGDSVCSSPSI
                     SSSTSPKLDPPPSPHANRKKHRRKKSTSNFKADGLSGTAEEQEENLEFIIVSLTGQTW
                     HFEATTYEERDAWVQAIESQILASLQSCESSKNKSRLTSQSEAMALQSIRNMRGNSHC
                     VDCDTQNPNWASLNLGALMCIECSGIHRNLGTHLSRVRSLDLDDWPMELIKVMSSIGN
                     ELANSVWEEGSQGRTKPSLDSTREEKERWIRAKYEQKLFLAPLPCTEFSLGQQLLRAT
                     AEEDLRTVILLLAHGSRDEVNETCGEGDGRTALHLACRKGNVVLAQLLIWYGVDVMAR
                     DAHGNTALAYARQASSQECIDVLLQYGCPDERFVLMATPNLSRKSNSRNNSSGRAPSV
                     I"
     exon            783..841
                     /gene="Agap1"
                     /gene_synonym="Centg2; Ggap1; mKIAA1099"
                     /inference="alignment:Splign:2.1.0"
     exon            842..929
                     /gene="Agap1"
                     /gene_synonym="Centg2; Ggap1; mKIAA1099"
                     /inference="alignment:Splign:2.1.0"
     exon            930..1015
                     /gene="Agap1"
                     /gene_synonym="Centg2; Ggap1; mKIAA1099"
                     /inference="alignment:Splign:2.1.0"
     exon            1016..1157
                     /gene="Agap1"
                     /gene_synonym="Centg2; Ggap1; mKIAA1099"
                     /inference="alignment:Splign:2.1.0"
     exon            1158..1292
                     /gene="Agap1"
                     /gene_synonym="Centg2; Ggap1; mKIAA1099"
                     /inference="alignment:Splign:2.1.0"
     exon            1293..1420
                     /gene="Agap1"
                     /gene_synonym="Centg2; Ggap1; mKIAA1099"
                     /inference="alignment:Splign:2.1.0"
     exon            1421..1576
                     /gene="Agap1"
                     /gene_synonym="Centg2; Ggap1; mKIAA1099"
                     /inference="alignment:Splign:2.1.0"
     exon            1577..1669
                     /gene="Agap1"
                     /gene_synonym="Centg2; Ggap1; mKIAA1099"
                     /inference="alignment:Splign:2.1.0"
     exon            1670..1774
                     /gene="Agap1"
                     /gene_synonym="Centg2; Ggap1; mKIAA1099"
                     /inference="alignment:Splign:2.1.0"
     exon            1775..1943
                     /gene="Agap1"
                     /gene_synonym="Centg2; Ggap1; mKIAA1099"
                     /inference="alignment:Splign:2.1.0"
     exon            1944..2102
                     /gene="Agap1"
                     /gene_synonym="Centg2; Ggap1; mKIAA1099"
                     /inference="alignment:Splign:2.1.0"
     exon            2103..2264
                     /gene="Agap1"
                     /gene_synonym="Centg2; Ggap1; mKIAA1099"
                     /inference="alignment:Splign:2.1.0"
     exon            2265..2419
                     /gene="Agap1"
                     /gene_synonym="Centg2; Ggap1; mKIAA1099"
                     /inference="alignment:Splign:2.1.0"
     exon            2420..2510
                     /gene="Agap1"
                     /gene_synonym="Centg2; Ggap1; mKIAA1099"
                     /inference="alignment:Splign:2.1.0"
     exon            2511..2733
                     /gene="Agap1"
                     /gene_synonym="Centg2; Ggap1; mKIAA1099"
                     /inference="alignment:Splign:2.1.0"
     exon            2734..2989
                     /gene="Agap1"
                     /gene_synonym="Centg2; Ggap1; mKIAA1099"
                     /inference="alignment:Splign:2.1.0"
     exon            2990..9581
                     /gene="Agap1"
                     /gene_synonym="Centg2; Ggap1; mKIAA1099"
                     /inference="alignment:Splign:2.1.0"
     regulatory      9556..9561
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Agap1"
                     /gene_synonym="Centg2; Ggap1; mKIAA1099"
                     /note="hexamer: AATAAA"
     polyA_site      9577
                     /gene="Agap1"
                     /gene_synonym="Centg2; Ggap1; mKIAA1099"
                     /note="major polyA site"
ORIGIN      
        1 gggggctggc cgggcagcgg cgggcggcgc tcggagcggt ctccgcggct ggcaaggcgc
       61 ctacagctcg gtcccggctt ggcggacggc gcagggcagc tgagggcccc ggcccgcgcg
      121 gtagcgcggc actcacaagc gcgcggaccc agccgcagcc gtgcgcccgg agcagagcgg
      181 ccactgcccg ggggcctcgg ccccggcgct cgcagcgccg tgctgcctgc gctttaatgg
      241 ctgctcggcc ggccggcgtg taggggtgcg ggcggctgcg gcgcgggaag gcgcccgagc
      301 gagcctcctc cgcggccggc cgcgctccat ggcgccgcgg tcttcccgga cggcccgctg
      361 acccgggacg ctggtccctg ctatgaactg agcccctcta ctgcttcatc ctcagcgttc
      421 ttttcttttt ctttttcttt ttcttttctt ttctttcttt ctttctttct ttctttcttt
      481 cttttttttt ttttttgcgc ctcctcggct ccgtgctccc cgggggctgc ggcgccccgg
      541 gtctcggtgg cccgcggggc tccggggcgc ggggcggcga cgacgggggg cgcgcggctt
      601 tgggcgccgc gcctgcacca tgaactacca gcagcagctg gccaactcgg ctgccatccg
      661 ggccgagatc cagcgcttcg agtcggtcca ccccaacatc tactccatct acgagctgct
      721 ggagcgcgtg gaggagccgg tgctgcagaa ccagattcgg gagcacgtca tcgccatcga
      781 agatgctttc gtgaacagcc aggaatggac actcagccga tcggtaccgg agctcaaagt
      841 gggaattgtg ggtaacctgg ccagtggcaa gtctgcgctg gtgcaccggt acctgacagg
      901 cacgtacgtg caggaggaat ctccagaagg tggcaggttc aagaaggaga ttgttgttga
      961 tggacagagt tatctgctgc tgattaggga tgaagggggc cccccggagg cacagttcgc
     1021 catgtgggtg gacgcggtca tctttgtctt cagcttggag gatgagatca gtttccagac
     1081 tgtctaccat tactacagcc gaatggccaa ctacaggaac accagtgaga tcccattagt
     1141 gctggtgggg acccaggatg ccataagttc caccaacccg agggtcatcg atgacgtcag
     1201 ggctcggaag ctctccaatg acctgaagag gtgcacgtac tatgagacgt gcgccaccta
     1261 tgggctcaac gtggagcggg tcttccagga cgttgcccag aagatcgttg ccacaaggaa
     1321 gaagcagcag ctatccatag gcccctgcaa gtccttaccc aactctccaa gccactcgtc
     1381 tgtgtgttct gcccaagtgt ccgcggtaca catcagccag acaagtaacg gaggtgggag
     1441 tttaagtgac tactcatcct ctgttccttc gaccccaagc accagccaga aagagcttcg
     1501 gatcgacgtc cctcccactg ccaacactcc aacgcctgtc cggaagcagt ccaagcgcag
     1561 gtccaaccta ttcacatcta ggaaagggag cgacccagac aaagagaaga aaggcctgga
     1621 gagccgtgcg gacagcatcg ggagcgggcg agccatccca attaaacagg gcatgctgct
     1681 gaagcgaagt ggcaagtcac tgaacaaaga gtggaaaaag aagtacgtca ccctgtgcga
     1741 caacggtgtg ctgacctacc accccagttt acatgattac atgcagaatg tccacggcaa
     1801 agagatagac ctcttgagga ccactgtgaa ggtgccgggg aagaggccgc cccgagccac
     1861 gtctgcctgt gcaccgatct ccagtcccaa aaccaacggc ctggccaagg acatgagcag
     1921 tttacacatc tcaccaaatt cagggaatgt cactagtgcg tctgggtctc agatggcaag
     1981 cggcatcagc ctggtctcct tcaacagccg acccgatggc atgcaccagc gctcctactc
     2041 agtctccagt gccgaccagt ggagtgacgc tacagtcatt gcaaactcag ccatcagcag
     2101 tgacacgggg ctgggtgact cggtgtgttc cagcccaagt atctccagca gcaccagccc
     2161 caagctcgac ccacccccct ccccccacgc caacagaaag aagcaccgga ggaagaaaag
     2221 taccagcaac ttcaaggctg acgggctctc aggcactgct gaagagcaag aagaaaactt
     2281 ggagtttatc attgtgtccc tcactggcca gacgtggcac tttgaagcca ccacatacga
     2341 ggagcgagac gcatgggtcc aagccatcga gagccagatc ctcgccagcc tgcagtcgtg
     2401 cgagagcagc aagaataagt cccgactaac cagccagagc gaagccatgg ccctgcagtc
     2461 catccgtaac atgagaggga actcgcactg cgtggactgt gacacccaaa accctaactg
     2521 ggccagtttg aacttgggag ccctcatgtg tatcgagtgc tcggggatcc accggaatct
     2581 cggcacccac ctctctcggg tcagatcgct agacctggac gactggccca tggagctgat
     2641 caaggtgatg tcatccatcg ggaatgagct ggccaacagc gtgtgggaag agggcagcca
     2701 gggacggaca aagccctcac tggactccac cagggaagaa aaggaaaggt ggatccgggc
     2761 caagtatgag cagaaactct tcctggcccc actgccatgc acagagttct ccctgggcca
     2821 gcagctgctg cgtgccactg ccgaggaaga cctgcggact gtcatcctac ttctggcaca
     2881 tgggtcccgg gacgaggtga atgagacctg tggggaagga gacggtcgca cggcgctgca
     2941 cctggcctgt cgcaagggca acgtggtcct ggctcagctg ctgatctggt acggagtgga
     3001 cgtcatggcc cgagacgccc acgggaacac agcactggcc tatgcccggc aggcctccag
     3061 ccaggagtgt attgacgtgc ttctacagta tggctgcccc gacgagcgct ttgtgctcat
     3121 ggctacccct aacctgtcca ggaagagcaa cagcaggaac aacagtagcg ggagggcgcc
     3181 cagtgtcatc tgagctcagc caacactaca cctgggatcc cacacccacc tgctccagaa
     3241 gttgcaacca ccacgtgagt ccctcagttc cctccctctt ccaggtggaa gccttccacc
     3301 cacctgccac tcactcaccc cacatgaaat ctccagacct ggacatcctc aaggggagaa
     3361 gaggaggctg caggccacag gacggaactc tttccccacc ctccggaagc aacgtaggag
     3421 gaccgaccac ctcccggctt tgaggatagc acacgacaac ggggacccct gttccagtgg
     3481 cctggagacg gggcaggggg aggggtagca aggagaaggg gcagcgtccc ctagctggca
     3541 gttaagcaca tcagtacatt tcacctcgac caaaaggagg cttagatttc acgtcttctc
     3601 gggggagctt gacggcataa atcagaagca agcacagttt gtcaggtctg aagcccctat
     3661 gatggtaggt gtcaaatcag ttgtagctaa tctgtccagg gagagtacca gcttcattac
     3721 acttgtatag tcgagttcct ccagcattac cgctgtttaa tagaacgtga ttagtcatca
     3781 ccgggaagaa ggcatattag ccgaggaggt agttacacgg cacgctccgg tgattgccac
     3841 gatatgattg cagtgcgcct agcagcatca tattatccta gacgtgtctc tccagccctt
     3901 ggagccctcc tttctaaatt cactgtcata atttagtatc tgtttaattt ttcagtccaa
     3961 agagaggaaa tcagttgctg ggtattattt gacttcagtc cccttacttg gtgcaaaaac
     4021 aaaatggaaa ataaataaat aagtaaataa agagtcactt ggaaattcta aacgaaatca
     4081 tgaattcggc tgaaaacagc tttcaaatat gttccaaaac taaatacata aaagactttt
     4141 ttttttctaa gagatttttc tgtactttgg ccacaacgga tgtgtcaggc ctcagtccac
     4201 aaggtctgga ttacccaggt ttctgtcgta gataagatgt gtgaaaatct atttgaataa
     4261 aagaagttca taaatatgca ttgatttttg cacagacaaa tggcacccat gttaatttat
     4321 aaattgagct ggatgggaac taataacgtg caactggttg agctcagagg gttacagatc
     4381 attgtccgtg ggaagtactc ccagcagcga acacttccat taatgtgata aacagctttt
     4441 taaaaggtgc gtatcagaat aagtcggtgg cacaatttgc ttattaaagt caggacagga
     4501 agcagggagg ggaaagaggg tatgagccct catcccagag cccagtttta gagccgagac
     4561 catcttccca gtcaaaccga ggggctgtgg caagaagggg tgatttctca aaacaccaga
     4621 cccacaaagg taccctgtga gcagctgcta gcggctctgg cacagccggc tcctgagtct
     4681 gtagactccg cacagcacgg cccgtctggc tctaacatcc gatttgccat cgtaggtcac
     4741 agggctcatc agataccgga caccttcagc acggtgatca gacaggaaca ccaaaccgtg
     4801 acactattta gttcttaaaa aaataaattg gggcagcctt gccagagagc cccccgcctg
     4861 gtgacagtgt ggcagggaga agtccatcac actggaagac aggacgctgg gcagagccac
     4921 caccggcagc ctgtggagac actccctacg cttatatatt gaactttata ttttgtaaga
     4981 gtttttcctc ctctttaact ttttaaaaaa agaaaacaaa aaacaaatgt aaagtagatg
     5041 ggaaggaaag tgaagcttca cacagccttt atatgtggtg cgcgtcttgt ggggatctcc
     5101 agtctgggca cagccggaac tgaccgggat ccagaaatgt cacagatgcg caagccgcgg
     5161 gtctgaggcc cctgaactca gtccataagc cctgcgtttg gtttctcttc ctgctctcca
     5221 tcactgtgtt gttactttaa atgtgaatag agaaaacaag ctgtcattgc ctatttttga
     5281 agaatgcgtt agcatccgac cccgcgatcc gcatacgcct ccacgtagcc tttaccatag
     5341 acttctatag agaacacaga tagagtataa atgagattct taaattattt cagtgtagct
     5401 aatccccact aggaatgctt ctgtcgtagg ggagcctact tcagaaagga agcagagtcc
     5461 tttgtgaggt ctctcttcgg ggtgtatatg agtgtgtaca tattattgtg tgtttatatg
     5521 atattttccc gaatgattcc ttgtttcaca tcatcctccg ctgcctgaat gcgcagttgc
     5581 tcgctctggt tccctccctc cctccctgac accatcagtt cttcctcgga acacatggcc
     5641 ccctcccctg ccagtttttg ccgtgctgaa ctgtccaaga gtagcccact tttcataagc
     5701 ctgagttgtg gcaatagata caagggtggt atcggagcct ccagggtttt ctgtgcagat
     5761 aaaggagaga cttgattcaa ctgaacttgt atttcacacc aggtccttcc ctcaacaaca
     5821 gcttcctgtt cccattttca tgatgacacc aaggctggga gaaacaggga cagggttatg
     5881 gctggcagtc ccagtagcaa acacttcccc taatgcaatg cgctttttaa agagcacatc
     5941 agaatgagat agtcacccgg ttgacttgct aaaatcaagg aaggaagtgg gaaaagtcaa
     6001 agaagctggt taagtggggg gaacacggaa catgtgcgtt ctcactccag acactgttag
     6061 agctgagacc ccacgcccac caggcccaag ccttcctgtt aaaggtgaat ctctcccttc
     6121 ttgttgagag gttcccagct gggaccagcg gcaaaggggc ctgtgactta tctgtcattt
     6181 gtcctgggga gtcactgata gaaccaggca catgccaggc tcacctgggg ctacctctgc
     6241 taagcttctg ataccctccc ccgggccgtg aacagtgtcg ggagggagag cagcctcatt
     6301 ctgagaaagc cactctaatg ttctgtctgg ttttacaaag agctaagcac tgctctccta
     6361 ctgtccccaa gtgtcactca agcctaagac gagcctcctc tcctgcaacc tcgtacctac
     6421 gctgttggct catcaagcct ccttctccct gtggttccta ccacgaggct gctggggtga
     6481 gcgcgcacgc acgcacgcac gcacgcactc gctcgaggag catacatgct ctgtcagagc
     6541 cctctgccct ttaactttga gcatcctaac acctcaggcc ttgggcttca ctgcctctat
     6601 cccgaggagc ttggccagag agtgaacccc ggggcactcc gtaacatcct cctttctctg
     6661 tcaagaccca cagtgagttt gtgtctaaac ttggtgagct ggagacttaa ctggccatga
     6721 gcctgatttg tgctcttggt tgccaaggtt tcggcccctc ctccctgcat tgacgctgtt
     6781 tcatgcctga gatgctccat ctctgtccct cctggagacg ccccacaaca gtacagctgg
     6841 gaggtcatca gggcctccaa actcctgaga ggctggcctc tctgagaagc cagcccttgt
     6901 ctttgggtgg gctctcctct gctcgtctgt ctccatcctt cacatcctac aaggtcccca
     6961 aggctttgct agaaatcccc ccaggatcgt atttatgact ttgaagcaaa caaattaaaa
     7021 agcaggaagg aattggagga aatgtgacat ggatgaacat ctctgcttaa aaaaaaataa
     7081 aaataacaac agaatttatc ccccactgct tctgttgtga agggcacagc tggaggccat
     7141 ttctgccttg tcactcacca gaaagctacc agggccattg gtgctcagtg tgccaccttc
     7201 tctttccatt gtctcttaga ttctaaatgg ccctactcct gccaggccct ggctagcagc
     7261 acaccaagat gtgtgcctgg gacataacag gaccaagtca tatccggtct aagagaacca
     7321 catgacccaa ctctttgtat ctggcctgtt ttgggccctt ctctttaaag cagctgagat
     7381 gaatggggtt ttagccaaaa tgtcacattc ctccttgaga ggtgagaaag ggaaggggag
     7441 aggccaggct actctgctgg tgccacccag cagtcgacca tagaccatgg gcttttagtc
     7501 ccacccctgg aagcaagaag cagggacagt tgattagaga ttccagtgcc tggagtcggg
     7561 gctcatctct cacttcatgg ggaaagatac catctggggc ctcagtgttt gaagaggcac
     7621 agactccttc tgctcagaag tcctgccctg acagggctct ttcagatgct gagaccccac
     7681 ttagtttctg gacctgctgt tctttggacc ctgggtcaga gttgcagtga gagaaggttt
     7741 catcatggat aattctagac agccaacaag gctctgtgtc cttaggtttt agcagatctg
     7801 ggtcaggcct gcttaagtgc cataagcctg gtggctccct gcccaaggca gtgcacactg
     7861 aggatccagg gagaagctga cctcccgccc ctcgtctcaa gaggccaagt ttcctataga
     7921 agaacagtat ttagtttgag tttgctcctg tctggtcaga gcatcttaac tgaggccttc
     7981 ctcttccata gtgagcctca ggtgggtgtg gatacccagc actggcagct actcgagggt
     8041 ccaagcaagg gcctcaggga gcaagtgagc gagagagaga gagagagaga gagagagaga
     8101 gagagagaga gaggaaggag agaggagaga ggtcagctca gggcttgtct ttattttgct
     8161 gggcacgtgg catcttttgg ggaccccccc aggggtcggg aatcaagcca taagatagac
     8221 tctcctgcca aagggaggac tgatctggaa ttctgtgtgc agggaatgac atggcctttg
     8281 tcttgttgcg ggtgaaatga aggaaccgag aggtatgagt gggtgagggg ggatgggatt
     8341 aagtcctgag gagagcccct taatacactg ttgctgggaa cagccgctct aggatcacat
     8401 tgtcccccag tgtctgggtt caggcctggg tccacactag cccacatgtt agtgtgggta
     8461 ttagagatgg ctctcaaatg cttgcaggaa gaaaagcata gcctcctgcg tggctcaggt
     8521 ggactcactg agggcatgca ccaaaacaga atttgtaaac agccctttgt tcacagctga
     8581 tgtgctcaga agatgctcgg ccccccaggc tcacccagat ggctagggac gggaccaagg
     8641 gtgctgtgag ttgggggtcc agcccacgtg tagcttctag ttcctgctct tgcatgcaca
     8701 cacgcacatt gttcacccac caaccaagtc ctgcagtttc cttaccaggg tagtcgagta
     8761 gagagacctg gctattgtgt gacagacagg caaaagatac cttcaaaaca tccttgtgat
     8821 cagagggaaa gacgcctgca cctcctgttg aaaatcatcc ctctcttgta tgtaaaacca
     8881 tcgctttccc tccccttatg gtggggctca ggggggccca gggggtagtt tgtttgtttt
     8941 ttttttttct aaaatgcacc accttctaag atgagaactg gtgctccagc cctgagccct
     9001 gaggactgaa agagtgttga gtcccaggaa ggcacagaag gtggctggcc cgttacaaca
     9061 gcctgagggt agaccctctt ctttccttcc cccagttcca ccttgagtct tcttgccaca
     9121 gggccttctt tgcaaaaaaa ataaaataaa aaaaaaatga aaccccagac aaggcagggc
     9181 accgctggca cttttctggg taggggcgag gaaggccatc tcgcctgcct ggccacacat
     9241 agaaggcaga agcccagtga gggcttctcc agaggtgggt cgccaattgg gcattttgat
     9301 attacctcat tccatgattt tacgtttccc gatgactgat gctttattta tgctgtttgt
     9361 cctgtaacta aaccacccac agacatttcg ctttgcttgt tcgttcatac gaccttgttc
     9421 gggtttaaat atgctggttt gtattttcct gccttgggat tttgtgtcag ctgtacagta
     9481 ttctaagggg aaaaggagac agagaagcgt gtaaagtaac ggagagaggt gcctataatg
     9541 gagagacctc tttctaataa agaaaagaaa atatgtatac a
//
