LOCUS       NM_145146               2022 bp    mRNA    linear   ROD 13-JUN-2024
DEFINITION  Mus musculus afamin (Afm), mRNA.
ACCESSION   NM_145146
VERSION     NM_145146.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Kim,B.J., Lee,Y.S., Lee,S.Y., Park,S.Y., Dieplinger,H., Ryu,S.H.,
            Yea,K., Choi,S., Lee,S.H., Koh,J.M. and Kim,G.S.
  TITLE     Afamin secreted from nonresorbing osteoclasts acts as a chemokine
            for preosteoblasts via the Akt-signaling pathway
  JOURNAL   Bone 51 (3), 431-440 (2012)
   PUBMED   22749887
  REMARK    GeneRIF: Afamin is one of osteoclast-derived chemokines that affect
            preosteoblasts through the activation of the Akt-signaling pathway.
REFERENCE   2  (bases 1 to 2022)
  AUTHORS   Liu,H., Ren,H. and Spear,B.T.
  TITLE     The mouse alpha-albumin (afamin) promoter is differentially
            regulated by hepatocyte nuclear factor 1alpha and hepatocyte
            nuclear factor 1beta
  JOURNAL   DNA Cell Biol 30 (3), 137-147 (2011)
   PUBMED   20979532
  REMARK    GeneRIF: Data indicate that HNF1alpha is a more potent activator of
            AFM promoter than is HNF1beta.
REFERENCE   3  (bases 1 to 2022)
  AUTHORS   Naidu,S., Peterson,M.L. and Spear,B.T.
  TITLE     Alpha-fetoprotein related gene (ARG): a new member of the albumin
            gene family that is no longer functional in primates
  JOURNAL   Gene 449 (1-2), 95-102 (2010)
   PUBMED   19733224
REFERENCE   4  (bases 1 to 2022)
  AUTHORS   Bernhard,O.K., Kapp,E.A. and Simpson,R.J.
  TITLE     Enhanced analysis of the mouse plasma proteome using
            cysteine-containing tryptic glycopeptides
  JOURNAL   J Proteome Res 6 (3), 987-995 (2007)
   PUBMED   17330941
REFERENCE   5  (bases 1 to 2022)
  AUTHORS   Ghesquiere,B., Van Damme,J., Martens,L., Vandekerckhove,J. and
            Gevaert,K.
  TITLE     Proteome-wide characterization of N-glycosylation events by
            diagonal chromatography
  JOURNAL   J Proteome Res 5 (9), 2438-2447 (2006)
   PUBMED   16944957
REFERENCE   6  (bases 1 to 2022)
  AUTHORS   Gabant,P., Forrester,L., Nichols,J., Van Reeth,T., De Mees,C.,
            Pajack,B., Watt,A., Smitz,J., Alexandre,H., Szpirer,C. and
            Szpirer,J.
  TITLE     Alpha-fetoprotein, the major fetal serum protein, is not essential
            for embryonic development but is required for female fertility
  JOURNAL   Proc Natl Acad Sci U S A 99 (20), 12865-12870 (2002)
   PUBMED   12297623
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC140220.4 and AC135240.2.
            
            On Sep 30, 2022 this sequence version replaced NM_145146.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC100597.1, AJ011080.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849385, SAMN00849386
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-131               AC140220.4         173851-173981
            132-180             AC140220.4         176806-176854
            181-313             AC140220.4         177061-177193
            314-525             AC140220.4         178633-178844
            526-658             AC140220.4         179692-179824
            659-756             AC140220.4         180408-180505
            757-886             AC140220.4         181246-181375
            887-1101            AC135240.2         127352-127566       c
            1102-1234           AC135240.2         113539-113671       c
            1235-1332           AC135240.2         111134-111231       c
            1333-1465           AC135240.2         110184-110316       c
            1466-1689           AC135240.2         108672-108895       c
            1690-1822           AC135240.2         107581-107713       c
            1823-1880           AC135240.2         106456-106513       c
            1881-2022           AC135240.2         105514-105655       c
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 44.78 cM"
     gene            1..2022
                     /gene="Afm"
                     /gene_synonym="Alf; alpha-Alb"
                     /note="afamin"
                     /db_xref="GeneID:280662"
                     /db_xref="MGI:MGI:2429409"
     exon            1..131
                     /gene="Afm"
                     /gene_synonym="Alf; alpha-Alb"
                     /inference="alignment:Splign:2.1.0"
     CDS             44..1870
                     /gene="Afm"
                     /gene_synonym="Alf; alpha-Alb"
                     /note="alpha-albumin"
                     /codon_start=1
                     /product="afamin precursor"
                     /protein_id="NP_660128.2"
                     /db_xref="CCDS:CCDS39141.1"
                     /db_xref="GeneID:280662"
                     /db_xref="MGI:MGI:2429409"
                     /translation="MRHLKLTGFIFFLLPLTESLALPTKPQDVDHFNATQKFIDENTT
                     YLAIIAFSQYVQEASFDEVETLVKVMLDYRDRCWADNTLPECSKTANDAIQDMLCDME
                     GLPQKHNFSHCCGKAGFPRRLCFFYNKKANVGFLPPFPTLDPEEKCQAYKNNSESFLH
                     LYMYEVARRNPFVFAPVLLAVAAWFEEAATTCCEQQQKATCFQAKAAPITQYLKASSS
                     YQRNVCGALIKFGPKVLNSINVAVFSKKFPKIGFKDLTTLLEDVSSMYEGCCEGDVVH
                     CIRSQSQVVNHICSKQDSISSKIKVCCEKKTLEREACIINANKDDRPEGLSLREAKFT
                     ESENVCQERDSDPDKFFAEFIYEYSRRHPDLSTPELLRITKVYMDFLEDCCSRENPAG
                     CYRHVEDKFNETTQRSLAMVQQECKQFQELGKDTLQRHFLVKFTKAAPQLPMEELVSL
                     SKEMVAALTTCCTLSDEFACVDNLADLVLGELCGVNTNRTINPAVDHCCKTDFAFRRH
                     CFEHLKADTTYELPSVSALVSALHTDWCQPRKEDLQNKKHRFLVNLVKWMPGITDEEW
                     LCLFTKFTAAREECSEVQEPESCFSPESSKTGDESQATEKQR"
     exon            132..180
                     /gene="Afm"
                     /gene_synonym="Alf; alpha-Alb"
                     /inference="alignment:Splign:2.1.0"
     exon            181..313
                     /gene="Afm"
                     /gene_synonym="Alf; alpha-Alb"
                     /inference="alignment:Splign:2.1.0"
     exon            314..525
                     /gene="Afm"
                     /gene_synonym="Alf; alpha-Alb"
                     /inference="alignment:Splign:2.1.0"
     exon            526..658
                     /gene="Afm"
                     /gene_synonym="Alf; alpha-Alb"
                     /inference="alignment:Splign:2.1.0"
     exon            659..756
                     /gene="Afm"
                     /gene_synonym="Alf; alpha-Alb"
                     /inference="alignment:Splign:2.1.0"
     exon            757..886
                     /gene="Afm"
                     /gene_synonym="Alf; alpha-Alb"
                     /inference="alignment:Splign:2.1.0"
     exon            887..1101
                     /gene="Afm"
                     /gene_synonym="Alf; alpha-Alb"
                     /inference="alignment:Splign:2.1.0"
     exon            1102..1234
                     /gene="Afm"
                     /gene_synonym="Alf; alpha-Alb"
                     /inference="alignment:Splign:2.1.0"
     exon            1235..1332
                     /gene="Afm"
                     /gene_synonym="Alf; alpha-Alb"
                     /inference="alignment:Splign:2.1.0"
     exon            1333..1465
                     /gene="Afm"
                     /gene_synonym="Alf; alpha-Alb"
                     /inference="alignment:Splign:2.1.0"
     exon            1466..1689
                     /gene="Afm"
                     /gene_synonym="Alf; alpha-Alb"
                     /inference="alignment:Splign:2.1.0"
     exon            1690..1822
                     /gene="Afm"
                     /gene_synonym="Alf; alpha-Alb"
                     /inference="alignment:Splign:2.1.0"
     exon            1823..1880
                     /gene="Afm"
                     /gene_synonym="Alf; alpha-Alb"
                     /inference="alignment:Splign:2.1.0"
     exon            1881..2022
                     /gene="Afm"
                     /gene_synonym="Alf; alpha-Alb"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2000..2005
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Afm"
                     /gene_synonym="Alf; alpha-Alb"
                     /note="hexamer: AATAAA"
     polyA_site      2022
                     /gene="Afm"
                     /gene_synonym="Alf; alpha-Alb"
                     /note="major polyA site"
ORIGIN      
        1 acattgctcc tttaaatgca tgaccttttt ctaaaattca aacatgagac acttaaaact
       61 tacaggtttt atttttttcc tgttgccttt gactgaatcc ctagccctac ccacaaagcc
      121 tcaggacgta gatcatttca atgctaccca gaaattcata gatgagaaca cgacatacct
      181 ggccatcatc gcgttttccc agtatgtcca ggaagcatcc tttgatgagg tggaaacgct
      241 ggtgaaagtc atgctggatt acagggatag gtgctgggct gacaacacac ttccagagtg
      301 ttcaaagaca gctaatgacg ctatccagga catgttatgt gacatggagg gactgccgca
      361 gaagcacaac ttttctcatt gctgcgggaa agccggcttt ccaagaagac tctgtttctt
      421 ctacaacaag aaagctaatg taggattctt gccccctttc cccactctgg atccagaaga
      481 gaaatgccaa gcttataaaa ataacagtga atcttttcta cacctctata tgtatgaggt
      541 tgccagaagg aacccctttg tcttcgcccc cgttcttcta gccgtggctg cttggtttga
      601 ggaggcagcg accacatgtt gtgaacagca acagaaagcc acctgctttc aagccaaggc
      661 agctcccatc acacaatatt taaaagcatc atcttcttat caaagaaatg tttgtggagc
      721 ccttataaag tttggaccca aggtcttaaa ctctataaac gttgctgtat tcagtaaaaa
      781 attccccaag attggattta aggaccttac caccctctta gaagatgttt cttccatgta
      841 tgaaggatgc tgtgaaggag atgttgtgca ctgcatccga agccagagcc aggttgtgaa
      901 ccatatttgt tcaaaacaag attccatctc aagtaaaatc aaagtgtgct gcgaaaagaa
      961 gaccctagaa cgtgaagcct gtataattaa tgcaaataaa gacgacagac cggagggctt
     1021 atctctcaga gaagccaaat tcactgagag cgaaaatgtg tgtcaagaac gagattctga
     1081 cccggacaag ttctttgctg agtttattta tgaatactcc aggagacatc ctgacctgtc
     1141 tacaccagag ctcctaagaa ttactaaagt gtatatggac ttcttagaag actgctgcag
     1201 cagagaaaac ccagcaggtt gttaccgcca tgtggaggat aaattcaatg agacaactca
     1261 gcgaagcctt gcaatggtgc agcaggagtg caaacagttc caggagctgg ggaaggacac
     1321 tctgcagcgc catttccttg tcaagttcac caaggcagcc cctcagctcc ccatggaaga
     1381 actggtctcc cttagtaagg aaatggtggc agctctgaca acctgttgca ctctcagtga
     1441 cgagttcgcc tgcgttgaca acttggcaga tttagttctt ggagagttat gtggggtaaa
     1501 tacaaatcga actatcaatc ctgctgtgga tcactgctgc aaaacagact tcgccttcag
     1561 aagacactgc tttgagcatc tgaaagctga tacaacgtat gagctcccat cagtctccgc
     1621 acttgtatct gctttgcata cagactggtg tcagcctcgt aaggaggacc ttcagaataa
     1681 gaaacacagg tttctggtca acttggtgaa gtggatgcca gggatcacgg atgaggagtg
     1741 gctgtgcttg ttcacaaagt tcactgctgc cagggaggag tgcagcgaag tgcaggagcc
     1801 tgagtcctgt ttcagtccag agagttcaaa aactggtgac gaaagtcaag ctacagagaa
     1861 acaaagataa ccgaccaaag ccagtgccag tttgcaggac cgtcctccgc cctgtggtgt
     1921 ggtgaaattc agcttttgag aagaatggaa tagaaggttt ttccgtaacc ctttcttgaa
     1981 tcatgttcct acaaaaggca ataaaatgaa aggataaagt ta
//
