LOCUS       NM_001033256            2538 bp    mRNA    linear   ROD 07-DEC-2024
DEFINITION  Mus musculus AFG2 AAA ATPase homolog B (Afg2b), transcript variant
            1, mRNA.
ACCESSION   NM_001033256 XM_141336
VERSION     NM_001033256.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2538)
  AUTHORS   Jelcick,A.S., Yuan,Y., Leehy,B.D., Cox,L.C., Silveira,A.C., Qiu,F.,
            Schenk,S., Sachs,A.J., Morrison,M.A., Nystuen,A.M., DeAngelis,M.M.
            and Haider,N.B.
  TITLE     Genetic variations strongly influence phenotypic outcome in the
            mouse retina
  JOURNAL   PLoS One 6 (7), e21858 (2011)
   PUBMED   21779340
  REMARK    Erratum:[PLoS One. 2011;6(10).
            doi:10.1371/annotation/f375292d-9fc7-4e3c-9dc7-af0aee76fa15]
REFERENCE   2  (bases 1 to 2538)
  AUTHORS   Sandell,L.L., Guan,X.J., Ingram,R. and Tilghman,S.M.
  TITLE     Gatm, a creatine synthesis enzyme, is imprinted in mouse placenta
  JOURNAL   Proc Natl Acad Sci U S A 100 (8), 4622-4627 (2003)
   PUBMED   12671064
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL772253.12 and AEKR01077154.1.
            
            On Mar 24, 2023 this sequence version replaced NM_001033256.4.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR13422597.287996.1,
                                           SRR13422591.484339.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-849               AL772253.12        66327-67175
            850-1129            AEKR01077154.1     1-280
            1130-1315           AEKR01077154.1     1040-1225
            1316-1474           AEKR01077154.1     4493-4651
            1475-1684           AEKR01077154.1     7710-7919
            1685-1877           AEKR01077154.1     9077-9269
            1878-2008           AEKR01077154.1     10654-10784
            2009-2134           AEKR01077154.1     11799-11924
            2135-2526           AEKR01077154.1     12570-12961
            2527-2538           AL772253.12        68698-68709
FEATURES             Location/Qualifiers
     source          1..2538
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 60.64 cM"
     gene            1..2538
                     /gene="Afg2b"
                     /gene_synonym="C130039A10Rik; Spata5l1"
                     /note="AFG2 AAA ATPase homolog B"
                     /db_xref="GeneID:214616"
                     /db_xref="MGI:MGI:3036261"
     exon            1..1129
                     /gene="Afg2b"
                     /gene_synonym="C130039A10Rik; Spata5l1"
                     /inference="alignment:Splign:2.1.0"
     CDS             56..2299
                     /gene="Afg2b"
                     /gene_synonym="C130039A10Rik; Spata5l1"
                     /EC_number="3.6.4.10"
                     /note="spermatogenesis-associated protein 5-like protein
                     1; ribosome biogenesis protein SPATA5L1; spermatogenesis
                     associated 5-like 1"
                     /codon_start=1
                     /product="ATPase family gene 2 protein homolog B"
                     /protein_id="NP_001028428.2"
                     /db_xref="GeneID:214616"
                     /db_xref="MGI:MGI:3036261"
                     /translation="MAPDSGPFPDGQLLKLLPVDPRDRDTQRCRLGPAAFRSLGARLG
                     SPLRISLPAGGCCLCTAWPRRDRVDGFVQLDPQCASPGARVATGRIGMDCLQPVPCPP
                     LRRLEVWPVLRPQAGAPSSAAVLEVVHELLRNRPVSRGHVVTTPPGVPGPVAALHVMG
                     GTPDPEPGGWVTPHTRITLSDKPPPQVEPPGEVTLGGLSETADSLRELLRLPLRYPLA
                     LAALGLAVPRGVLLAGPPGVGKTQLVRAVARETGAELLAVSAPALQGSRPGETEENVR
                     RIFQRAQELASRGPSLLFLDEVDALCPRRGGPHRAPESRVVAQVLTLLDGIHRDREFV
                     VVGATNRPDELDPALRRPGRFDREVVIGTPTLKQREAILQVITSKMPISSHIDLGLLA
                     EMTVGYVGADLTALCREAAMCALLKNEKNQDSPKIEETDFLEAFKKIQPSSFRSSTGL
                     MDIKPVGWEQIGGLEDVKLKLKQCVEWPLKFPQEFARMGLTQPKGLLLYGPPGCAKTT
                     LVRALATGCHCSFVSVCGADLFSPFVGDSEKVLSQVFRQARANTPALVFLDEIDSVLG
                     SRSVGTSGCDARERVLSVLLNELDGVGVRTVERRGSKASQQECQEILSRSVMIVVATN
                     RPDVLDDALLRPGRLDKIIYVPPPDQEGRLSILKVCTNNMPVGPDVSLENLAAETCFF
                     SGADLRNLCKEAALFALQENGLEATTVRQEHFTEALKTVKPSLTLKELTFYENLFKKG
                     LSNLEDD"
     exon            1130..1315
                     /gene="Afg2b"
                     /gene_synonym="C130039A10Rik; Spata5l1"
                     /inference="alignment:Splign:2.1.0"
     exon            1316..1474
                     /gene="Afg2b"
                     /gene_synonym="C130039A10Rik; Spata5l1"
                     /inference="alignment:Splign:2.1.0"
     exon            1475..1684
                     /gene="Afg2b"
                     /gene_synonym="C130039A10Rik; Spata5l1"
                     /inference="alignment:Splign:2.1.0"
     exon            1685..1877
                     /gene="Afg2b"
                     /gene_synonym="C130039A10Rik; Spata5l1"
                     /inference="alignment:Splign:2.1.0"
     exon            1878..2008
                     /gene="Afg2b"
                     /gene_synonym="C130039A10Rik; Spata5l1"
                     /inference="alignment:Splign:2.1.0"
     exon            2009..2134
                     /gene="Afg2b"
                     /gene_synonym="C130039A10Rik; Spata5l1"
                     /inference="alignment:Splign:2.1.0"
     exon            2135..2538
                     /gene="Afg2b"
                     /gene_synonym="C130039A10Rik; Spata5l1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2519..2524
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Afg2b"
                     /gene_synonym="C130039A10Rik; Spata5l1"
                     /note="hexamer: AATAAA"
     polyA_site      2538
                     /gene="Afg2b"
                     /gene_synonym="C130039A10Rik; Spata5l1"
                     /note="major polyA site"
ORIGIN      
        1 atccggcagc cgggtggatt gctgccgggc tttccccggc gtggttcccc gagtcatggc
       61 tccggactcg gggcccttcc ccgacggaca acttttgaag ctgctgccgg tggatccccg
      121 ggaccgggac acccagcgct gccgcctggg gccggccgcc ttccgcagct tgggtgcgcg
      181 cctgggctcg ccgctgagga tctcgttgcc cgccggcggc tgctgcctct gcacggcctg
      241 gccgcggcgc gacagggtgg atggctttgt gcagctggac ccgcagtgcg ctagccccgg
      301 ggcccgggtg gccacgggga ggataggtat ggactgcttg caacccgtgc cctgtccgcc
      361 cctgcggcgc ctagaggtgt ggccggtgct gcggccccag gcgggagccc cgagttccgc
      421 cgcggtgctg gaggtagtgc acgagctgct gcgcaaccgg cctgtgtctc ggggacacgt
      481 ggtgaccacc ccgccgggtg tccccggccc tgtcgcagct ctgcacgtga tgggcgggac
      541 accggaccca gagcccggcg ggtgggtcac accgcatacc cgcatcacgc tcagtgacaa
      601 gcctccgcca caggtcgagc ctcccgggga ggtgaccctt gggggtctgt cggagaccgc
      661 cgactcccta cgggagctgc tccgcctgcc gctgcgctac ccgctcgccc tagccgcgct
      721 ggggctggct gtgccccgcg gggtgctcct ggcgggcccc ccgggagtgg gcaagaccca
      781 gttagtgcgg gccgtggcgc gcgagactgg cgccgagctg ctggcggtga gcgccccggc
      841 gttgcagggc tcccggcccg gggagaccga ggagaacgtg cggcgcatct tccaacgagc
      901 ccaggagctg gccagccgcg gacccagcct cctcttcctg gacgaagtgg atgccctgtg
      961 tccccggaga ggcggcccgc accgagctcc cgagagccga gtggtggccc aggtgttgac
     1021 gctgctggac ggcatccaca gggacaggga gtttgtggtt gtgggagcca ccaaccggcc
     1081 agacgagtta gacccagcgc ttcgcaggcc gggcaggttt gacagagagg ttgtcattgg
     1141 aactcccacc cttaaacaaa gagaagcaat tctgcaagtg attacttcaa agatgcccat
     1201 ctccagtcat attgatttag gtctcctggc agaaatgacg gttggctatg ttggtgctga
     1261 cctgacagca ctctgtaggg aggctgccat gtgtgccctt ctcaagaatg agaagaacca
     1321 agacagtcct aaaattgagg aaacagattt tcttgaagct tttaaaaaga ttcagccctc
     1381 atcatttcga agttccactg gactgatgga catcaagcct gttggctggg agcagatagg
     1441 tggccttgaa gacgtaaagc tgaagttaaa gcagtgtgtt gagtggcctc tgaagttccc
     1501 tcaggagttt gctaggatgg gtctgacaca gccaaagggc cttctcctgt atggtccccc
     1561 tggatgtgct aaaaccactc tggtgagggc cctggccaca ggctgccact gctccttcgt
     1621 gtcagtgtgc ggagctgatc tcttctctcc ttttgttgga gactcagaaa aagtcttgtc
     1681 tcaggtattt cgacaagcaa gagccaatac tccagcgctt gtgtttctgg atgaaattga
     1741 ctcagtcttg gggtctcgct cagtcggcac ctcgggatgt gacgctcggg agcgagttct
     1801 ttcagttctg ctgaacgaac tcgatggtgt gggagttagg acggtagaga gaagagggag
     1861 taaagcaagc caacaggagt gtcaagaaat tcttagtcgg agtgtcatga ttgtcgtggc
     1921 aacaaatagg cctgatgtgt tagacgatgc cttgctacgg cctggaagat tagataagat
     1981 catctacgtc ccccctcctg atcaagaggg caggctttct attttaaaag tctgtacaaa
     2041 caacatgcca gtagggcctg atgtttcact agaaaaccta gcagcagaaa cctgcttttt
     2101 ctcaggagct gatcttagaa acctctgcaa agaagctgcc ttgtttgctc tgcaagaaaa
     2161 cggactggaa gcaaccacag tgagacagga gcacttcaca gaagcactca agactgtgaa
     2221 gccatcctta actctgaaag agttgacctt ctatgaaaac ttatttaaga aaggactttc
     2281 taacttggaa gatgattaaa ttttaatttt attcagtttt tcatatgaag tagtttgcaa
     2341 gtttccactt tcatgaaagg gagaagggag aggtcggggg ggagagggga gggagggaga
     2401 aagggaggga gggagagaga gggagagaga gagagagaga gagagagaga gagagagaga
     2461 gatgagacct tgcctgataa agaccatctt acttctagaa gagattaaga caccataata
     2521 aaaagcgcat gtgtgtga
//
