LOCUS       NM_001163511            3258 bp    mRNA    linear   ROD 11-FEB-2025
DEFINITION  Mus musculus AFG2 AAA ATPase homolog A (Afg2a), transcript variant
            1, mRNA.
ACCESSION   NM_001163511
VERSION     NM_001163511.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3258)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3258)
  AUTHORS   Jones,M.R., Dilai,S., Lingampally,A., Chao,C.M., Danopoulos,S.,
            Carraro,G., Mukhametshina,R., Wilhelm,J., Baumgart-Vogt,E., Al
            Alam,D., Chen,C., Minoo,P., Zhang,J.S. and Bellusci,S.
  TITLE     A Comprehensive Analysis of Fibroblast Growth Factor Receptor 2b
            Signaling on Epithelial Tip Progenitor Cells During Early Mouse
            Lung Branching Morphogenesis
  JOURNAL   Front Genet 9, 746 (2019)
   PUBMED   30728831
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3258)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643.. PMID: 29144450]
REFERENCE   4  (bases 1 to 3258)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   5  (bases 1 to 3258)
  AUTHORS   Fenske,T.S., McMahon,C., Edwin,D., Jarvis,J.C., Cheverud,J.M.,
            Minn,M., Mathews,V., Bogue,M.A., Province,M.A., McLeod,H.L. and
            Graubert,T.A.
  TITLE     Identification of candidate alkylator-induced cancer susceptibility
            genes by whole genome scanning in mice
  JOURNAL   Cancer Res 66 (10), 5029-5038 (2006)
   PUBMED   16707424
REFERENCE   6  (bases 1 to 3258)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   7  (bases 1 to 3258)
  AUTHORS   Liu,Y., Black,J., Kisiel,N. and Kulesz-Martin,M.F.
  TITLE     SPAF, a new AAA-protein specific to early spermatogenesis and
            malignant conversion
  JOURNAL   Oncogene 19 (12), 1579-1588 (2000)
   PUBMED   10734318
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL669927.9, BY106106.1, AK011111.2 and CO043526.1.
            
            On Jul 14, 2015 this sequence version replaced NM_001163511.1.
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK144998.1, AK011111.2 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849381, SAMN00849382
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-330               AL669927.9         59148-59477
            331-367             BY106106.1         2-38
            368-3240            AK011111.2         2-2874
            3241-3258           CO043526.1         1-18                c
FEATURES             Location/Qualifiers
     source          1..3258
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="3"
                     /map="3 18.46 cM"
     gene            1..3258
                     /gene="Afg2a"
                     /gene_synonym="2510048F20Rik; Spaf; Spata5"
                     /note="AFG2 AAA ATPase homolog A"
                     /db_xref="GeneID:57815"
                     /db_xref="MGI:MGI:1927170"
     exon            1..551
                     /gene="Afg2a"
                     /gene_synonym="2510048F20Rik; Spaf; Spata5"
                     /inference="alignment:Splign:2.1.0"
     CDS             392..3073
                     /gene="Afg2a"
                     /gene_synonym="2510048F20Rik; Spaf; Spata5"
                     /EC_number="3.6.4.10"
                     /note="isoform 1 is encoded by transcript variant 1;
                     spermatogenesis-associated protein 5; spermatogenesis
                     associated 5; spermatogenesis-associated factor protein;
                     ATPase family protein 2 homolog; ribosome biogenesis
                     protein SPATA5; ATPase family gene 2 protein homolog A"
                     /codon_start=1
                     /product="ATPase family gene 2 protein homolog A isoform
                     1"
                     /protein_id="NP_001156983.1"
                     /db_xref="CCDS:CCDS50895.1"
                     /db_xref="GeneID:57815"
                     /db_xref="MGI:MGI:1927170"
                     /translation="MSSKKNRKRQKEGAEGASPSLSAAPSRSGTSTPFAQPSPAAPGM
                     LVVTNFLEKVDGKVPKTFQNSLVHLGLNTMKSANICIGRPVLLTSLDGKQEVYTAWPV
                     AGFPGGKVGLSEMAQKNVGVRAGETIQVQPLLGAVLQAQEMDLALSDKDKEINEEELT
                     GCILRKLDGKIVLPGNFLYCTFYGRLCKLQVLQVKGTDGTTLGKLQSASGTDAQGMAS
                     EHSSMESSDVDLSFQLSQLDLKEPQSPSSQSTPCKPTNDRTVNKAGEVLLDVTQSPRD
                     GSGLGLEESTGLKCSFDSSKEGNTQPVSEEKLLKPASAGTKSNTDTFYFISSTTRINL
                     RKICTNSKEQDSQFKVTYDMIGGLNSQLKAIREIIELPLKQPELFKSYGIPAPRGLLL
                     YGPPGTGKTMIARAVANEVGAYVSVINGPEIISKFYGETEARLRQIFAEATLRHPSII
                     FIDELDALCPKREGAQSEVEKRVVASLLTLMDGIGSEGSEGRVLVLGATNRPQALDAA
                     LRRPGRFDKEIEIGIPNAQDRLDILQKLLRRVPHLLTKAELLRLANNAHGYVGADLKA
                     LCNEAGLHALRRVLRKQPNLPDSKVAGMVKITLNDFLQGMNDIRPSAMREVAIDVPNV
                     SWSDIGGLENIKLKLKQAVEWPLKHPKSFNRMGIQPPKGVLLYGPPGCSKTMIAKALA
                     NESGLNFLAIKGPELMNKYVGESERAVREIFRKARAVAPSIIFFDELDALAVERGSSS
                     GAGNVADRVLAQLLTEMDGIEQLKNVTVLAATNRPDRIDKALMRPGRIDRIIYVPLPD
                     AATRREILNLQFHSMPISNEVDLDELVLQTDTYSGAEIIAVCKEAALLALEENIKADC
                     IMKRHFTQALSIVTPRIPESLRRFYEDYQEKSGLHTV"
     exon            552..676
                     /gene="Afg2a"
                     /gene_synonym="2510048F20Rik; Spaf; Spata5"
                     /inference="alignment:Splign:2.1.0"
     exon            677..834
                     /gene="Afg2a"
                     /gene_synonym="2510048F20Rik; Spaf; Spata5"
                     /inference="alignment:Splign:2.1.0"
     exon            835..893
                     /gene="Afg2a"
                     /gene_synonym="2510048F20Rik; Spaf; Spata5"
                     /inference="alignment:Splign:2.1.0"
     exon            894..1538
                     /gene="Afg2a"
                     /gene_synonym="2510048F20Rik; Spaf; Spata5"
                     /inference="alignment:Splign:2.1.0"
     exon            1539..1668
                     /gene="Afg2a"
                     /gene_synonym="2510048F20Rik; Spaf; Spata5"
                     /inference="alignment:Splign:2.1.0"
     exon            1669..1725
                     /gene="Afg2a"
                     /gene_synonym="2510048F20Rik; Spaf; Spata5"
                     /inference="alignment:Splign:2.1.0"
     exon            1726..1849
                     /gene="Afg2a"
                     /gene_synonym="2510048F20Rik; Spaf; Spata5"
                     /inference="alignment:Splign:2.1.0"
     exon            1850..2105
                     /gene="Afg2a"
                     /gene_synonym="2510048F20Rik; Spaf; Spata5"
                     /inference="alignment:Splign:2.1.0"
     exon            2106..2260
                     /gene="Afg2a"
                     /gene_synonym="2510048F20Rik; Spaf; Spata5"
                     /inference="alignment:Splign:2.1.0"
     exon            2261..2470
                     /gene="Afg2a"
                     /gene_synonym="2510048F20Rik; Spaf; Spata5"
                     /inference="alignment:Splign:2.1.0"
     exon            2471..2524
                     /gene="Afg2a"
                     /gene_synonym="2510048F20Rik; Spaf; Spata5"
                     /inference="alignment:Splign:2.1.0"
     exon            2525..2604
                     /gene="Afg2a"
                     /gene_synonym="2510048F20Rik; Spaf; Spata5"
                     /inference="alignment:Splign:2.1.0"
     exon            2605..2731
                     /gene="Afg2a"
                     /gene_synonym="2510048F20Rik; Spaf; Spata5"
                     /inference="alignment:Splign:2.1.0"
     exon            2732..2896
                     /gene="Afg2a"
                     /gene_synonym="2510048F20Rik; Spaf; Spata5"
                     /inference="alignment:Splign:2.1.0"
     exon            2897..3241
                     /gene="Afg2a"
                     /gene_synonym="2510048F20Rik; Spaf; Spata5"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3217..3222
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Afg2a"
                     /gene_synonym="2510048F20Rik; Spaf; Spata5"
                     /note="hexamer: AATAAA"
     polyA_site      3241
                     /gene="Afg2a"
                     /gene_synonym="2510048F20Rik; Spaf; Spata5"
                     /note="major polyA site"
ORIGIN      
        1 aagcttgagc tggacttgcc atttggtaga gcttagtgga gctgactgtc ctcgacgggt
       61 ctggaacaga gaggagaaac tttcggattt tgttgttgtt actttaagcc caaccacatt
      121 cgcttcggga ctaggcaaag cagcggaaca gccgaagtcc acttttcaag ggttcataac
      181 gtcaacggcg gtgcctacgc ctagcccttc tttctttccg aaacggtgtc ttgcgagacc
      241 ctcgctctcc gcctccgcct cgtgggtggc gcttctacgc ggagcattac ggcgtgcgcg
      301 ccgcgccgct gacctctgac ccggaagttt gatccccgtg ccgcgcacgt tgagccggct
      361 cggggctgct tttctccgac agcctgcggt catgtcttcc aagaagaacc gaaagcggca
      421 gaaggagggc gcggagggcg cctcgccctc gctctctgcg gctccgtccc gctcagggac
      481 ctcgactcct ttcgcccagc cgagcccggc ggcgccgggg atgctggtgg tgaccaactt
      541 ccttgagaaa gtagatggca aagttcctaa gacattccag aattcccttg ttcatcttgg
      601 acttaacact atgaagtctg caaacatatg tataggtcgg ccagtgctcc ttaccagttt
      661 ggatggaaag caggaggttt atacagcctg gccagttgca ggatttcctg gaggcaaggt
      721 tggcctgagt gaaatggcac agaagaatgt gggtgtgaga gctggtgaga ccatccaggt
      781 ccagcctctt ttgggtgctg tgcttcaggc tcaggaaatg gacttggcgc tgagtgacaa
      841 agataaggaa attaatgaag aagaactgac tggttgtatt ctgagaaaac tagatggcaa
      901 gattgtttta ccaggcaact ttctatactg tacattctat ggacgactct gcaagttaca
      961 agtgttgcaa gtgaaaggga cagatggcac aacattggga aagcttcaga gtgcctctgg
     1021 cactgatgcc cagggaatgg cctctgaaca ttctagcatg gaaagcagcg atgtggatct
     1081 atccttccag ctaagccagt tagatctgaa agaacctcag agcccatcat cacagagtac
     1141 tccctgtaaa cccactaatg acagaactgt gaataaagct ggtgaagttt tgttggatgt
     1201 cacacagagc cctagggatg gaagtggact tggactagag gaaagcacag gtcttaagtg
     1261 tagttttgac tcatccaaag aaggaaatac acaacctgtc agtgaagaga aactactaaa
     1321 acctgctagt gcagggacaa agagcaatac tgatactttt tattttattt cttcaacaac
     1381 aagaatcaat cttcgaaaaa tttgtacaaa ttcaaaagaa caagacagtc agttcaaagt
     1441 aacttatgac atgattggag gcttaaacag tcagctgaaa gcaataagag aaataattga
     1501 attgcctctc aagcagcctg aactttttaa gagttacgga attccagcgc ctagagggtt
     1561 attactctat ggtcctccag gtaccggaaa gacaatgatt gctagggctg ttgctaatga
     1621 agttggagct tatgtttctg taattaatgg tcctgaaatt ataagcaaat tctacggtga
     1681 gactgaagca aggttacgcc agatatttgc tgaagctact ctacgacacc catcaattat
     1741 ttttattgat gaactggatg cactttgccc taaaagagaa ggagctcaga gtgaagtgga
     1801 aaagagagtt gtagcttcac tcttaacatt gatggatggc attggctcag aaggaagtga
     1861 aggacgggta ttagtccttg gggccacaaa tcgccctcaa gcactagatg ctgcactcag
     1921 acgacctggg agatttgata aagagattga gattggcatt cctaatgccc aagaccggct
     1981 tgatattctc cagaaactac tccgaagggt gccacatttg ctcactaaag ctgagcttct
     2041 gaggttggca aataatgccc atggttatgt tggcgcagac ttgaaagcgt tgtgtaatga
     2101 ggcaggtctg catgccttgc gaagagtcct gaggaaacag cctaacctcc ctgatagcaa
     2161 ggtggctggc atggtgaaga ttactctaaa tgatttctta caggggatga atgacatcag
     2221 gcctagtgct atgagagagg tagcaattga tgtcccaaat gtttcctggt cagatattgg
     2281 aggactggaa aatatcaaac tgaaattgaa acaggctgtg gaatggccct tgaaacatcc
     2341 taagtctttc aacagaatgg gtattcagcc acccaaagga gttctcttgt atgggccacc
     2401 tggatgctca aaaacaatga tagcaaaagc tttggccaat gaaagtgggt tgaactttct
     2461 agctataaag gggcccgaat taatgaataa atatgttggt gaatctgaaa gagcagttag
     2521 agagatattc cgtaaagcaa gagcagtggc accgtccatt atcttttttg atgaacttga
     2581 tgccctggct gttgaaaggg gcagttcttc aggtgccggg aatgttgcag accgtgtttt
     2641 ggctcagctg ttaacggaaa tggatgggat tgaacagcta aaaaatgtga cagttttggc
     2701 agctactaat cgcccagata gaatagacaa ggctttgatg aggcctggaa gaattgacag
     2761 gatcatttat gtgcccttac cggacgcagc aaccagaagg gaaatactga acctgcagtt
     2821 ccactcaatg ccgatcagta acgaggttga cctggatgaa cttgtcctcc aaactgacac
     2881 ctactcagga gcagagatca tagcagtctg caaggaggcg gctctcctag ctctggaaga
     2941 aaacattaaa gctgactgca ttatgaagag gcatttcact caagccttga gcattgtgac
     3001 acccaggatc ccagagtcac tgagacgctt ttatgaagat taccaagaaa agagtggact
     3061 acacacagtc taaaaaatac ttatgtgcat atattttcta gagaaaagtt tgttttcaaa
     3121 tttttgtctt gagagtgttt actgtttctt aataggtaaa tgtttgaaat accttcactg
     3181 gagttgaaga gccaatgttt tgactcattt gagtataata aacatttctc tgggcagttc
     3241 aaaaaaaaaa aaaaaaaa
//
