LOCUS       NM_145743               2530 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus AFG1 like ATPase (Afg1l), transcript variant 1, mRNA.
ACCESSION   NM_145743
VERSION     NM_145743.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2530)
  AUTHORS   Kumar,R.A., Chan,K.L., Wong,A.H., Little,K.Q., Rajcan-Separovic,E.,
            Abrahams,B.S. and Simpson,E.M.
  TITLE     Unexpected embryonic stem (ES) cell mutations represent a concern
            in gene targeting: lessons from 'fierce' mice
  JOURNAL   Genesis 38 (2), 51-57 (2004)
   PUBMED   14994267
REFERENCE   3  (bases 1 to 2530)
  AUTHORS   Chalhoub,N., Benachenhou,N., Rajapurohitam,V., Pata,M., Ferron,M.,
            Frattini,A., Villa,A. and Vacher,J.
  TITLE     Grey-lethal mutation induces severe malignant autosomal recessive
            osteopetrosis in mouse and human
  JOURNAL   Nat Med 9 (4), 399-406 (2003)
   PUBMED   12627228
REFERENCE   4  (bases 1 to 2530)
  AUTHORS   Abrahams,B.S., Mak,G.M., Berry,M.L., Palmquist,D.L., Saionz,J.R.,
            Tay,A., Tan,Y.H., Brenner,S., Simpson,E.M. and Venkatesh,B.
  TITLE     Novel vertebrate genes and putative regulatory elements identified
            at kidney disease and NR2E1/fierce loci
  JOURNAL   Genomics 80 (1), 45-53 (2002)
   PUBMED   12079282
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY295329.1, AK044595.1 and BC089595.1.
            
            On Apr 11, 2007 this sequence version replaced NM_145743.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC089595.1, AF520417.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-125               BY295329.1         1-125
            126-2510            AK044595.1         2-2386
            2511-2530           BC089595.1         2361-2380
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="10"
                     /map="10 22.88 cM"
     gene            1..2530
                     /gene="Afg1l"
                     /gene_synonym="CG8520; Lace1"
                     /note="AFG1 like ATPase"
                     /db_xref="GeneID:215951"
                     /db_xref="MGI:MGI:2148801"
     exon            1..303
                     /gene="Afg1l"
                     /gene_synonym="CG8520; Lace1"
                     /inference="alignment:Splign:2.1.0"
     CDS             165..1607
                     /gene="Afg1l"
                     /gene_synonym="CG8520; Lace1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     lactation elevated protein 1; lactation elevated 1"
                     /codon_start=1
                     /product="AFG1-like ATPase isoform 1"
                     /protein_id="NP_665686.2"
                     /db_xref="CCDS:CCDS23811.1"
                     /db_xref="GeneID:215951"
                     /db_xref="MGI:MGI:2148801"
                     /translation="MAASWSPLVTLRSAARSRLTGRGVGCGARVVAIPPPAPGPGKPL
                     WKAYTVQTSEGVRPTAASEARLKALAVCHGPLDHYDFLIKSQELREDEHQRRVVQCLQ
                     KLQEDLKGYSIEEGGLFSKLFSRNKPPKGLYVYGDVGTGKTMVMDMFYAYVETKRKKR
                     VHFHGFMLDVHRRIHHLKQSLPKRKAGFMAKSYDPIAPIAEEISQETSLLCFDEFQVT
                     DIADAMILKQLFENLFKNGVVVVATSNRPPEDLYKNGLQRANFVPFIAVLKEYCDTLQ
                     LDSGVDYRKRELAPAGKLYYLTSEADVEAVVDKLFDELAQKQNDLTSPRILKVQGREL
                     RLNKACGSVADCTFEELCERPLGASDYLELSKNFDTVIIRNIPQFSLAKRTQARRFIT
                     LIDNFYDFKVRIICSASAPISSLFLHQHQDSESDQSRILMDDLGLSQDSAGLSMFTGE
                     EEIFAFQRTISRLTEMQTEQYWIEGDRSRK"
     exon            304..527
                     /gene="Afg1l"
                     /gene_synonym="CG8520; Lace1"
                     /inference="alignment:Splign:2.1.0"
     exon            528..579
                     /gene="Afg1l"
                     /gene_synonym="CG8520; Lace1"
                     /inference="alignment:Splign:2.1.0"
     exon            580..681
                     /gene="Afg1l"
                     /gene_synonym="CG8520; Lace1"
                     /inference="alignment:Splign:2.1.0"
     exon            682..812
                     /gene="Afg1l"
                     /gene_synonym="CG8520; Lace1"
                     /inference="alignment:Splign:2.1.0"
     exon            813..912
                     /gene="Afg1l"
                     /gene_synonym="CG8520; Lace1"
                     /inference="alignment:Splign:2.1.0"
     exon            913..971
                     /gene="Afg1l"
                     /gene_synonym="CG8520; Lace1"
                     /inference="alignment:Splign:2.1.0"
     exon            972..1054
                     /gene="Afg1l"
                     /gene_synonym="CG8520; Lace1"
                     /inference="alignment:Splign:2.1.0"
     exon            1055..1125
                     /gene="Afg1l"
                     /gene_synonym="CG8520; Lace1"
                     /inference="alignment:Splign:2.1.0"
     exon            1126..1226
                     /gene="Afg1l"
                     /gene_synonym="CG8520; Lace1"
                     /inference="alignment:Splign:2.1.0"
     exon            1227..1367
                     /gene="Afg1l"
                     /gene_synonym="CG8520; Lace1"
                     /inference="alignment:Splign:2.1.0"
     exon            1368..1481
                     /gene="Afg1l"
                     /gene_synonym="CG8520; Lace1"
                     /inference="alignment:Splign:2.1.0"
     exon            1482..2513
                     /gene="Afg1l"
                     /gene_synonym="CG8520; Lace1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2491..2496
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Afg1l"
                     /gene_synonym="CG8520; Lace1"
                     /note="hexamer: AATAAA"
     polyA_site      2513
                     /gene="Afg1l"
                     /gene_synonym="CG8520; Lace1"
                     /note="major polyA site"
ORIGIN      
        1 ggaaggcgtg aagggcaggc gttgcagcct ttctaagctt gaggtcatct ctctttaacg
       61 ctctctcatt ttcaggcgtg gcctcctgga gcgccacagc acccggctgc tccgtcgcgt
      121 cttctgggtt acaataaagt tttcctccct gtacggagct caagatggcg gcctcctggt
      181 cgcccttggt tactctgcgc tccgccgcgc gcagccggct gactgggaga ggtgttgggt
      241 gcggggcgcg ggtcgtcgca atccctcctc cggcccctgg ccctgggaaa cccctgtgga
      301 aagcctacac agtccagaca tccgagggtg tacgcccaac agccgcttct gaggcccgtt
      361 tgaaagcctt ggctgtttgc cacgggcctc tggaccacta cgactttctg atcaaatctc
      421 aggagctgag ggaggatgaa catcaaagaa gagtcgttca gtgtttgcag aagttacagg
      481 aggaccttaa aggatacagc atagaggaag gagggctctt ttcaaagctt ttttcaagga
      541 acaaacctcc aaagggcctc tatgtttatg gagacgttgg tacaggaaaa acaatggtga
      601 tggacatgtt ttatgcatat gtggaaacga agagaaagaa gcgggtccat tttcacggct
      661 tcatgctgga cgtgcacaga aggatacacc accttaaaca gagtttgcca aaaaggaagg
      721 cgggattcat ggctaagtca tatgacccga tagctcccat agccgaggaa atcagtcaag
      781 aaacttctct cctatgtttt gatgaatttc aggtcactga catcgccgac gccatgattc
      841 tgaaacagct ttttgaaaat ctgttcaaaa acggggtcgt cgttgtggca acatccaaca
      901 gaccaccgga agatctctat aaaaatggac tccagagggc taactttgtg ccatttatag
      961 ctgtcctgaa ggaatattgt gatacgctcc agctggattc tggggtagat taccggaaaa
     1021 gggaactcgc ccctgcagga aaactctact acctcacaag tgaggctgat gtggaggccg
     1081 tcgtggataa gttgtttgat gagctggctc agaaacaaaa tgatttaact agcccaagga
     1141 ttctgaaagt gcaaggcaga gagctgcggc tgaataaagc ctgtggaagc gttgccgact
     1201 gcacatttga agagctgtgt gagagaccac ttggagcaag tgactatttg gaattatcaa
     1261 agaattttga tacggtaatc atacgaaaca ttccgcagtt ttcactggca aagaggacgc
     1321 aagcgagaag attcatcact ctcattgata acttctatga cttcaaggtg cgcattatct
     1381 gctccgcttc agctccgatc tcaagcttgt ttctgcatca gcatcaggac agtgagtcgg
     1441 accaaagcag aatcctgatg gatgatttgg ggctgagcca ggactcagcc gggctctcca
     1501 tgtttacggg ggaggaggaa atcttcgcct ttcagcgcac catttcccgg ctcacagaga
     1561 tgcagacgga gcagtactgg attgaagggg acagaagcag gaagtaactg ccactttgac
     1621 aagattaaaa ggaaaaggtg actggttagg gctgcgagac agtgagatgt cactgcgaga
     1681 cgtcactgcg tcaccgagac gtgacgggga tcatttcgtc atcatcaatg cactttgccc
     1741 tctggactgc tgcgtctgtg gcagtggatg gacacactga agaattctgt ctcctttgta
     1801 gatccgagtt tatttataaa gtggaatcat cactcttgaa gattactgca aaccctgaca
     1861 tgtgtgcgct gtctgaatca tgaacgcaca ggcagagtat gcaggatgtc tgtgcacctc
     1921 ccagcttcgg ctgcacagta gtcacactgt agaaatacat tcgctgtaag aaacaagtgg
     1981 catcctactg ttcaggttaa aacaaagaaa gccttctgaa cataactgaa gggaaaagca
     2041 ctttacgttg gcctctgtat tcctgtcagt tagttggtta ggaatggcaa taacctttgg
     2101 gcctcaaggt ttcctcagct ctagaaagaa cacgtgtcta accagcagat ccaggttagc
     2161 aggcagtgcc tgagcccatg aggacatagg ttataaagcc atcaggtaag gagcaagtag
     2221 aaaattccag ctgattggca ggtctcccct ctgacagaca gccaaaatgg ggcaggttcc
     2281 aggatcctga ttggtcgagt gctggaggag gcagagccag agccacaccc acaggagcat
     2341 ggaccatccc tccctccccc catcacccaa ggtttccact ctgttcacta gcttctgctt
     2401 gcactacaga gtccactccc tgtccttgat cctgagtcac agagttgggc cttgtaggaa
     2461 actggaagga cacttgataa atcttctata aataaaagtt ggtttctcct tcaaaaaaaa
     2521 aaaaaaaaaa
//
