LOCUS       NM_033565              10165 bp    mRNA    linear   ROD 05-DEC-2024
DEFINITION  Mus musculus AF4/FMR2 family, member 4 (Aff4), transcript variant
            1, mRNA.
ACCESSION   NM_033565
VERSION     NM_033565.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 10165)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 10165)
  AUTHORS   Liu,A., Chen,Y., Zhong,D., Wang,C., Yu,M., Liu,C., Yang,Z., Chen,W.
            and Yin,K.
  TITLE     CircRNA AFF4 induced by KDM1A promotes osteogenic differentiation
            through FNDC5/Irisin pathway
  JOURNAL   Mol Med 28 (1), 134 (2022)
   PUBMED   36401176
  REMARK    GeneRIF: CircRNA AFF4 induced by KDM1A promotes osteogenic
            differentiation through FNDC5/Irisin pathway.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 10165)
  AUTHORS   Chen,Y., Li,Q., Liu,Y., Chen,X., Jiang,S., Lin,W., Zhang,Y.,
            Liu,R., Shao,B., Chen,C., Yuan,Q. and Zhou,C.
  TITLE     AFF4 regulates cellular adipogenic differentiation via targeting
            autophagy
  JOURNAL   PLoS Genet 18 (9), e1010425 (2022)
   PUBMED   36149892
  REMARK    GeneRIF: AFF4 regulates cellular adipogenic differentiation via
            targeting autophagy.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 10165)
  AUTHORS   Li,S., Liang,X., Liang,Y., Li,L., Gan,J., Cao,L. and Zou,Y.
  TITLE     Identification of the transcription factor, AFF4, as a new target
            of miR-203 in CNS
  JOURNAL   Int J Biol Macromol 181, 919-927 (2021)
   PUBMED   33878354
  REMARK    GeneRIF: Identification of the transcription factor, AFF4, as a new
            target of miR-203 in CNS.
REFERENCE   5  (bases 1 to 10165)
  AUTHORS   Li,J., Lee,Y.K., Fu,W., Whalen,A.M., Estable,M.C., Raftery,L.A.,
            White,K., Weiner,L. and Brissette,J.L.
  TITLE     Modeling by disruption and a selected-for partner for the nude
            locus
  JOURNAL   EMBO Rep 22 (3), e49804 (2021)
   PUBMED   33369874
REFERENCE   6  (bases 1 to 10165)
  AUTHORS   Urano,A., Endoh,M., Wada,T., Morikawa,Y., Itoh,M., Kataoka,Y.,
            Taki,T., Akazawa,H., Nakajima,H., Komuro,I., Yoshida,N.,
            Hayashi,Y., Handa,H., Kitamura,T. and Nosaka,T.
  TITLE     Infertility with defective spermiogenesis in mice lacking AF5q31,
            the target of chromosomal translocation in human infant leukemia
  JOURNAL   Mol Cell Biol 25 (15), 6834-6845 (2005)
   PUBMED   16024815
  REMARK    GeneRIF: AF5q31 (Aff4) is a transcriptional regulator in testicular
            somatic cells and is essential for male germ cell differentiation
            and survival.
REFERENCE   7  (bases 1 to 10165)
  AUTHORS   Oliver,P.L., Bitoun,E., Clark,J., Jones,E.L. and Davies,K.E.
  TITLE     Mediation of Af4 protein function in the cerebellum by Siah
            proteins
  JOURNAL   Proc Natl Acad Sci U S A 101 (41), 14901-14906 (2004)
   PUBMED   15459319
REFERENCE   8  (bases 1 to 10165)
  AUTHORS   Gu,Y., McIlwain,K.L., Weeber,E.J., Yamagata,T., Xu,B.,
            Antalffy,B.A., Reyes,C., Yuva-Paylor,L., Armstrong,D., Zoghbi,H.,
            Sweatt,J.D., Paylor,R. and Nelson,D.L.
  TITLE     Impaired conditioned fear and enhanced long-term potentiation in
            Fmr2 knock-out mice
  JOURNAL   J Neurosci 22 (7), 2753-2763 (2002)
   PUBMED   11923441
REFERENCE   9  (bases 1 to 10165)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
REFERENCE   10 (bases 1 to 10165)
  AUTHORS   Wenderfer,S.E., Slack,J.P., McCluskey,T.S. and Monaco,J.J.
  TITLE     Identification of 40 genes on a 1-Mb contig around the IL-4
            cytokine family gene cluster on mouse chromosome 11
  JOURNAL   Genomics 63 (3), 354-373 (2000)
   PUBMED   10704283
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CJ064959.1, BC138999.1 and AI536326.1.
            
            On Jul 18, 2009 this sequence version replaced NM_033565.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF190449.1, BC138999.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-327               CJ064959.1         2-328
            328-9594            BC138999.1         1-9267
            9595-10165          AI536326.1         2-572               c
FEATURES             Location/Qualifiers
     source          1..10165
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 31.93 cM"
     gene            1..10165
                     /gene="Aff4"
                     /gene_synonym="AF5Q31; Alf4; Laf4l; MCEF"
                     /note="AF4/FMR2 family, member 4"
                     /db_xref="GeneID:93736"
                     /db_xref="MGI:MGI:2136171"
     exon            1..363
                     /gene="Aff4"
                     /gene_synonym="AF5Q31; Alf4; Laf4l; MCEF"
                     /inference="alignment:Splign:2.1.0"
     exon            364..490
                     /gene="Aff4"
                     /gene_synonym="AF5Q31; Alf4; Laf4l; MCEF"
                     /inference="alignment:Splign:2.1.0"
     CDS             368..3850
                     /gene="Aff4"
                     /gene_synonym="AF5Q31; Alf4; Laf4l; MCEF"
                     /note="isoform 1 is encoded by transcript variant 1; ALL1
                     fused gene from 5q31"
                     /codon_start=1
                     /product="AF4/FMR2 family member 4 isoform 1"
                     /protein_id="NP_291043.1"
                     /db_xref="CCDS:CCDS24676.1"
                     /db_xref="GeneID:93736"
                     /db_xref="MGI:MGI:2136171"
                     /translation="MNREDRNVLRMKERERRNQEIQQGEDAFPPSSPLFAEPYKVTSK
                     EDKLSSRIQSMLGNYDEMKDYIGDRSIPKLVAIPKPAVPTTTDEKANPNFFEQRHGGS
                     HQSSKWTPVGPAPSTSQSQKRSSALQSGHSSQRSGAGGSGASSSGQRHDRDSYSSSRK
                     KGQHGSEHSKSRSSSPGKPQAVSSLSSSHSRSHGNDHHSKEHQRSKSPRDPDANWDSP
                     SRGPFSSGQHSSQSFPPSLMSKSSSMLQKPTAYVRPMDGQESVEPKLSSEHYSSQSHG
                     NSMTELKPSSKAHLTKLKIPSRPLDASVSGDVSCVDEILKEMTHSWPPPLTAIHTPCK
                     TEPSKFPFPTKESQQSNFGPGEQKRYSTAKTSNGHQSKSMLKDDLKLSSSEDSDGEQD
                     CDKTMPRSTPGSNSEPSHHNSEGADNSRDDSSSHSGSESSSGSDSESESSSSDSEANE
                     PSQSASPEPEPPPTNKWQLDNWLNKVNPHKVSPASSVDSNIPSSQAYKKEGREQGTAS
                     NYTDPGGTKETSSATPGRDSKTIQKGSESGRGRQKSPAQSDSTTQRRTVGKKQPKKPE
                     KSAAEEPRGGLKIESETPVDMAASMPSSRHKAATKGSRKPNIKKESKSSPRPTAEKKK
                     YKSASKPSQKSREIIETDTSSSDSDGSESLPPSSQTPKYPESNRTPVKPSSVEEEDSF
                     FRQRMFSPMEEKELLSPLSEPDDRYPLIVKIDLNLLTRIPGKPYKETEPPKGEKKNVP
                     EKHSREVQKQASEKASNKGKRKHKNDDDTRASESKKPKTEDKNSSGHKPSSSRESSKQ
                     SSTKEKDLLPSPAGPILSKDSKTEHGSRKRTVSQSSSLKSSGTSSKENSGSSSKSSSS
                     STAKQKKTEGKGPSSSKEAKEKAPNSSSNCPPSTPTSESSKPRRTKLAFDDRNYSADH
                     YLQEAKKLKHNADALSDRFEKAVYYLDAVVSFIECGNALEKNAQESKSPFPMYSDTVE
                     LIKYTMKLKNYLAPDATAADKRLTVLCLRCQSLLYLRLFKLKKENALKYSKTLTEHLK
                     NSYSNSQAPSPGLGSKAVGMPSPVSPKLSPGNSGSYSSGGSSASASGSSVTIPQKIHQ
                     MAASYVQVTSNFLYATEIWDQAEQLSKEQKEFFAELDKVMGPLIFNASIMTDLARYTR
                     QGLHWLRQDAKLIS"
     exon            491..1270
                     /gene="Aff4"
                     /gene_synonym="AF5Q31; Alf4; Laf4l; MCEF"
                     /inference="alignment:Splign:2.1.0"
     exon            1271..1315
                     /gene="Aff4"
                     /gene_synonym="AF5Q31; Alf4; Laf4l; MCEF"
                     /inference="alignment:Splign:2.1.0"
     exon            1316..1402
                     /gene="Aff4"
                     /gene_synonym="AF5Q31; Alf4; Laf4l; MCEF"
                     /inference="alignment:Splign:2.1.0"
     exon            1403..1439
                     /gene="Aff4"
                     /gene_synonym="AF5Q31; Alf4; Laf4l; MCEF"
                     /inference="alignment:Splign:2.1.0"
     exon            1440..1485
                     /gene="Aff4"
                     /gene_synonym="AF5Q31; Alf4; Laf4l; MCEF"
                     /inference="alignment:Splign:2.1.0"
     exon            1486..1540
                     /gene="Aff4"
                     /gene_synonym="AF5Q31; Alf4; Laf4l; MCEF"
                     /inference="alignment:Splign:2.1.0"
     exon            1541..1578
                     /gene="Aff4"
                     /gene_synonym="AF5Q31; Alf4; Laf4l; MCEF"
                     /inference="alignment:Splign:2.1.0"
     exon            1579..1741
                     /gene="Aff4"
                     /gene_synonym="AF5Q31; Alf4; Laf4l; MCEF"
                     /inference="alignment:Splign:2.1.0"
     exon            1742..2659
                     /gene="Aff4"
                     /gene_synonym="AF5Q31; Alf4; Laf4l; MCEF"
                     /inference="alignment:Splign:2.1.0"
     exon            2660..2748
                     /gene="Aff4"
                     /gene_synonym="AF5Q31; Alf4; Laf4l; MCEF"
                     /inference="alignment:Splign:2.1.0"
     exon            2749..2995
                     /gene="Aff4"
                     /gene_synonym="AF5Q31; Alf4; Laf4l; MCEF"
                     /inference="alignment:Splign:2.1.0"
     exon            2996..3090
                     /gene="Aff4"
                     /gene_synonym="AF5Q31; Alf4; Laf4l; MCEF"
                     /inference="alignment:Splign:2.1.0"
     exon            3091..3154
                     /gene="Aff4"
                     /gene_synonym="AF5Q31; Alf4; Laf4l; MCEF"
                     /inference="alignment:Splign:2.1.0"
     exon            3155..3291
                     /gene="Aff4"
                     /gene_synonym="AF5Q31; Alf4; Laf4l; MCEF"
                     /inference="alignment:Splign:2.1.0"
     exon            3292..3363
                     /gene="Aff4"
                     /gene_synonym="AF5Q31; Alf4; Laf4l; MCEF"
                     /inference="alignment:Splign:2.1.0"
     exon            3364..3457
                     /gene="Aff4"
                     /gene_synonym="AF5Q31; Alf4; Laf4l; MCEF"
                     /inference="alignment:Splign:2.1.0"
     exon            3458..3501
                     /gene="Aff4"
                     /gene_synonym="AF5Q31; Alf4; Laf4l; MCEF"
                     /inference="alignment:Splign:2.1.0"
     exon            3502..3722
                     /gene="Aff4"
                     /gene_synonym="AF5Q31; Alf4; Laf4l; MCEF"
                     /inference="alignment:Splign:2.1.0"
     exon            3723..10165
                     /gene="Aff4"
                     /gene_synonym="AF5Q31; Alf4; Laf4l; MCEF"
                     /inference="alignment:Splign:2.1.0"
     regulatory      9430..9435
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Aff4"
                     /gene_synonym="AF5Q31; Alf4; Laf4l; MCEF"
                     /note="hexamer: ATTAAA"
     polyA_site      9454
                     /gene="Aff4"
                     /gene_synonym="AF5Q31; Alf4; Laf4l; MCEF"
                     /note="major polyA site"
     regulatory      9546..9551
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Aff4"
                     /gene_synonym="AF5Q31; Alf4; Laf4l; MCEF"
                     /note="hexamer: AATAAA"
     polyA_site      9574
                     /gene="Aff4"
                     /gene_synonym="AF5Q31; Alf4; Laf4l; MCEF"
                     /note="major polyA site"
     regulatory      10003..10008
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Aff4"
                     /gene_synonym="AF5Q31; Alf4; Laf4l; MCEF"
                     /note="hexamer: ATTAAA"
     regulatory      10007..10012
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Aff4"
                     /gene_synonym="AF5Q31; Alf4; Laf4l; MCEF"
                     /note="hexamer: AAGAAA"
     polyA_site      10026
                     /gene="Aff4"
                     /gene_synonym="AF5Q31; Alf4; Laf4l; MCEF"
     polyA_site      10034
                     /gene="Aff4"
                     /gene_synonym="AF5Q31; Alf4; Laf4l; MCEF"
ORIGIN      
        1 gtcagtccca gtcccggctg cgggagtcga gtcgtcgccg ccgccgccgc caccgccgcc
       61 cccagcggac ggggcgggga tcttggtgcc agggccccgt cagcccgccc ccgcctcagt
      121 cacccgtccg gggctcggcc gggccgtcat ggagcggggc ggaaccctgg cgtccgcggt
      181 gtgaggtgcg ccgcctcggc tccccgtggc ctccggatcg gccgctgccc ctcgccccag
      241 gcagccggtc gccgcctcct gggcgggccc gaatgcgcgt ccggtgaagg tgcaggccgg
      301 gcgccgccgc cgccgcagcc gcggtcggga aatggttcgg gcctagcgga gcggggactg
      361 gagcaacatg aaccgtgaag accggaatgt gctgcgcatg aaagaaaggg aaaggcggaa
      421 tcaggaaatt cagcagggtg aagacgcctt cccacctagt tctcctctct ttgctgagcc
      481 atacaaagtc actagcaaag aagataagtt atcaagtcgg attcagagca tgcttggaaa
      541 ctacgatgaa atgaaggatt acataggaga cagatcgata ccaaagcttg ttgcaattcc
      601 caagcctgca gtaccaacga ccacagacga gaaagcaaac ccaaattttt ttgagcagag
      661 acatggagga tctcatcaga gtagcaaatg gactcctgta ggccccgccc ccagcacctc
      721 tcagtctcag aaacgctcct ctgccctgca gagtggacac agcagccaac ggagtggtgc
      781 aggagggagt ggcgccagca gcagtggcca gaggcatgac cgtgactcct acagcagtag
      841 caggaaaaaa ggccagcacg gctcagagca ctccaaatct cgatcctcaa gccctggaaa
      901 accccaggct gtttcttcat taagttctag tcattccagg tctcatggga atgatcacca
      961 tagcaaggaa catcagcgct ccaaatcacc tcgggaccct gatgccaact gggattctcc
     1021 ttcccgcgga cctttttcaa gtgggcaaca ttcaagtcag tctttcccac cttctttgat
     1081 gtcaaaatct agttcaatgt tacagaaacc cactgcctat gtacggccca tggatggaca
     1141 ggagtctgtg gaaccaaagc tgtcctctga gcactacagc agccagtctc atggtaatag
     1201 catgactgag ctgaagccca gcagcaaagc acatctcacc aagctgaaaa ttccttcccg
     1261 acctttggat gcatcggttt ctggtgatgt aagctgtgta gatgaaatac ttaaagagat
     1321 gacgcattca tggcctcccc ctctaacggc tattcataca ccatgcaaaa cagaaccttc
     1381 caaattccct tttccaacta aggagtcgca acagtctaac tttggccctg gagaacaaaa
     1441 aagatacagt actgctaaaa catcaaatgg acaccagtca aaatctatgt taaaagatga
     1501 cttaaaacta agcagcagtg aagacagtga tggggaacag gattgtgata agacaatgcc
     1561 aaggagtaca ccaggaagta actctgaacc ttcacaccat aacagtgagg gagcagacaa
     1621 ctccagggat gactcgagca gccacagtgg atctgagagc agctctgggt ctgactcgga
     1681 gagtgagagc agctccagtg acagtgaggc taatgagccg tcgcagagcg catctcctga
     1741 gcctgaacca ccaccaacaa acaagtggca acttgataat tggttgaata aagtgaaccc
     1801 acataaagta tccccagctt cctctgtaga cagcaacatc ccatcttctc aagcctacaa
     1861 gaaagaaggc cgggagcagg gcactgccag taactacaca gatccagggg ggacaaaaga
     1921 aacgagctct gccaccccag gacgagactc taaaaccatc caaaagggat cagaaagtgg
     1981 ccgtgggagg cagaagtctc ctgcacagag cgacagtacc actcagagga gaactgtcgg
     2041 caaaaaacaa cccaaaaaac ctgagaagtc agctgctgaa gagcctcgtg gaggcctgaa
     2101 gatagaaagt gagacccctg tggacatggc tgccagcatg ccctccagca ggcacaaagc
     2161 agccaccaag ggctcgagga aacccaacat caaaaaggaa tcaaaatctt ctcctcgacc
     2221 tacggcagag aagaagaagt acaagtcagc cagtaaaccg tcccagaagt ctcgggagat
     2281 catagagact gacacctcgt cctcagactc ggacggcagc gagagcctgc ctccctcctc
     2341 acagactcct aagtaccctg aaagcaacag gactcctgtc aaaccctcct cagtggagga
     2401 agaagatagc ttttttcggc aacgaatgtt ctctcctatg gaagagaagg agctcctttc
     2461 cccactcagt gagcctgatg acaggtatcc actcattgtg aagattgacc tcaatcttct
     2521 gactcgaata cccgggaagc cttacaaaga aacagagcca cccaaggggg aaaagaaaaa
     2581 tgttccagag aaacactcga gagaggtgca gaagcaagcc tcagaaaagg cttccaacaa
     2641 aggcaagagg aaacacaaga atgatgatga tacccgagcc agtgagagca aaaaacccaa
     2701 aacagaggac aagaactcat caggccacaa gccatccagc agcagagagt catctaagca
     2761 gagtagtaca aaagaaaagg acttgctacc ttcacctgct gggcccattc tttcaaaaga
     2821 ttcaaaaaca gagcatggtt ctcggaagag gactgtcagt cagtcgtcgt ccttaaagtc
     2881 cagtgggacc agcagcaaag agaacagtgg cagcagcagc aagagcagct cctcctccac
     2941 agcgaagcag aagaagactg aaggcaaggg tcccagcagc tccaaggagg ccaaggagaa
     3001 ggctccaaat agctcctcta attgtcctcc gtctacacca acttctgagt cttcgaagcc
     3061 tcggagaaca aagcttgcct ttgatgacag aaattattca gcagaccatt atttacaaga
     3121 agcaaaaaaa ctcaaacaca atgcagatgc tttgtctgac aggtttgaga aagccgtgta
     3181 ctaccttgat gctgtagtgt ctttcattga gtgtgggaat gcattagaga agaacgctca
     3241 ggaatccaag tccccattcc ctatgtactc agacacggtg gagctcatta agtacactat
     3301 gaaactaaag aattatttgg caccagatgc tacagctgca gacaagagac tcacagtact
     3361 ttgcctgaga tgccagtcat tattgtacct gaggctgttc aaactgaaga aggagaacgc
     3421 tctcaagtac tccaagacgc taactgagca cctgaagaat tcttacagta attctcaagc
     3481 accttcaccc ggcttgggga gcaaagctgt tgggatgccc tcccctgttt ctccaaaact
     3541 gtcaccaggc aattctggaa gttactcttc tgggggaagt agtgcttcag caagtggttc
     3601 ttcagtgacc attccacaga aaatccacca gatggcagca agctatgtcc aggttacatc
     3661 taacttcctc tatgccacag aaatttggga ccaagctgaa cagctttcca aagaacaaaa
     3721 agaattcttt gctgaactgg acaaagtgat gggccctctc atctttaatg caagcatcat
     3781 gacagatctg gctcgttaca cccggcaggg actgcattgg cttcggcagg atgccaagct
     3841 gatatcttga actgaacata ctctcgttgc ctctgatttt tttccacaac actgtgtcac
     3901 atcacaaagg aaaactgcca taacacacca cctagtcgac actaagaatg aggaatggtt
     3961 ttctcctctt tggtttatgt tgttgtttct ataacccaaa gctatcatat caattgaccc
     4021 tttgaagagt cccaatgtga caagtattta aatgcttcta aaccttcagt acatcagcaa
     4081 gatggtttta gtaattggaa ttccagttgc aattcataaa taatcaagta aaattctatt
     4141 tattttcatt ttttttcaag ttcccagatt ggagcttgtt taaagcttag tatttctgcc
     4201 tataaattta ttctgttaat atttagcata aacttaatgt aggcatttta tttatatatt
     4261 ttgggagctg tgttacttaa tgtcttctaa gataagagtt ggcttgtcca agacaggact
     4321 tgttctctct acagctactc acctatgttc tttcaagtct tactacagtt ttattattgt
     4381 aggatttatg cttggtatta aggatgctct tccacatgtg aaaagtcatt taaacatgag
     4441 aagacggcat tctgcatggg tgcagaaatg gagtgggtct ggtttagtat gcattatttc
     4501 aaaactgtga atgtgtgtta gtaacactag caatcacaag gtgactacat tgcatctaat
     4561 gcattacaaa aaagcagata gatttgctgg ggaagcactg aatggcttca taaaggacaa
     4621 tcttaaacta tgagtttgtt aaaaccttat attctttttt gttttactca tttgacactt
     4681 gattgaaaca gagtaaatta ctcctaaaaa cagtgatcca gtttctgaag gctgcagcct
     4741 gtattcattg ctaatgtttg aaggttaaag tctgtggatt tcccctatgg tggagagatt
     4801 gtgatgctca gatctgacag ttcaggaact ggagcttgaa aggaggtgtc ccaatgtcta
     4861 atgtggagaa aattaatttc tctttccaaa gatcctcatc aatgatgtat gcttttgaca
     4921 ggatcagaaa actcacagta gcagctgagc atggttttgt atatctgtaa tcccaacact
     4981 ggggagggat atcaggaggg tcaggaggtc agaatcatcc tcaagctaca gaaagctaga
     5041 cagtgagttt ggggctagcc tgagctacac aatcccttgt cacattaaag aaagaaaatg
     5101 tatactagga aagtaccaaa cttggctcat tgtgagcttt cttggaaagg gggagatgat
     5161 agagacaggg cttaggaagc cagctctgta acagttcctt atcaaactac tgttcccgca
     5221 aggtgcgcat agcagtgaag ggcgaggatg tctctcttcc tctatacttc tttttagtga
     5281 ccccgtcatg tctggtgtgc cttaaatctt aactttaaat gataactcta tggattgttt
     5341 acagataaag ttttatgctg ggaccagatt agccatgcat ggttggtcct ctacaataat
     5401 aatttcaaat cctctttgat tttaaatctt cttttagagt cacattaaga ctccttcaga
     5461 aaagctagta ctttggtacc tgatttagtg taatcaattg ggtataatgc tgcttcaaaa
     5521 aatggctttt ccttctcatt ccaaaaatcc atccaattag ggagaaaaat cccctgttag
     5581 cagatgattg ttgagacttg aatggaaagg aggaagaggg gatagcaacc ttcacgcagc
     5641 agtgtcgcaa atcctctctg actccttcac cacctgtttc tgaagatagt gaactttgtc
     5701 ctgcagtctc atatctacag agcttaactg ctttgtgtct cattattttt acatagtgtt
     5761 gagggttgat acctatagct tatgctgaat tgaacatttt tcccttggag aaattgcctc
     5821 tgtaccttcc tactttaatc gtagtgtatt tacggaatgc ttcttcagag gccatcggtt
     5881 cctctcttgt gctagtaacc tccatggtct aacagctgtg tgtcctgtcc ttgcctcttc
     5941 tctctcactc tggcttctcc atttcaggga ggccttagaa gtagagcaag caccctctcc
     6001 ctgtgctttc agccccgctg ctgcagttcg gggctctgct ttccagctct gggctgtgtg
     6061 cagcgcattc tagtgcgcct ctgacgtcat gctaaagcag agtttcctac gtgttcatgg
     6121 tgctgcttta agatgttttc cctttaaaac tgtcagttct tggagaatga aatggttgaa
     6181 cctttcattg tggaggattt tcctcaccac atagaaatgg gcaatggatc aaaatctgat
     6241 cctaagcgat tgcatttttg atgtcatagt gtgtgagaat acactctgtg tctcttctca
     6301 gatgttttgt ctcagtcatg tccagcacat accttggact cctttacaag tgaccatccc
     6361 tccctctcgg taggtgccct cagtgttttt tagccattta aagctgattt gacaagtggc
     6421 tacaaactta gtctgaaact tgtattcact ctatagttag taatagaaat agcaactatt
     6481 gttaatgctc agttcactgc tcttaagatc acatttctat ggcaggaggc agcagcatgc
     6541 accattggac tctggacagg tgatcacagg atcacagtga tgcagctgtc acagtagtcc
     6601 tgcctgggag aggctgggct gtgggagcac tcccctccat gcattcaaga ttacacagcc
     6661 tagcccatgt cccattctgg ctggagaaca cccagccctt tccttgctca gtgacacctt
     6721 tttctgggca agaagttatg aagtcacttt attatctgta agtgcttcaa ataaagtcac
     6781 agagctgctg aagggagaag gttgaccact actgatttaa ttctccactg tcaaagcaat
     6841 tcagagtaat gtgtgcatgc gtccacagtt gctgtttttt ctgggaagct cttgcactag
     6901 ggatttacag gcacctgggc tgcactttga atgacacact tgtggcttta gagtagctgt
     6961 tattgttttg ttttaaaatt gttcttcgtt tcttttcatt aatggtttaa tcagctagat
     7021 tagaaagcat catttgtatt taacagctga aactttggta cgttgacggt tatcgagtga
     7081 gcttgcattg tgtagccagg gcctacagag gctcttgtca ggaatacaag cactgcctgg
     7141 agctggtgct cagaggagct tctagtgcaa ccaggaatag ctccgtgggg tgatgcctag
     7201 ctcaattgag aagggggatg ctgtacactc ttctaggtat gagggtatgc gactttggat
     7261 cttaaaatta tgtacacata cacactttat atatatgtac gtatgtatgt atgaaaacat
     7321 ggaattagtt tgtcaaaaat gtgtgtgttt agtattttag cttagtgcaa ctatttccac
     7381 attatttatt aaattgatct aagacacttt gttcttggca ccttgaatat taacattcaa
     7441 ggatgcaatg tgtattcctt tagattgtta aagcttaatt actatgattt gtagtaaatt
     7501 aacttttgaa atatatttga gcccttctgt agtataatag ggctcttaca gggtgggaaa
     7561 gattttaatt ttccaatagc taattgaaca aaatggcttc attgtatatt ttgatttata
     7621 ggagtctgta aggtgtctgg gtcccaagga catctactga ggatgactgt tttaggtggg
     7681 tggaatggtg tttagactat accttagtga cttggaaagc cagagcacct agttcaaatc
     7741 cggactccta taactcattc ctctaggatg tggaatacaa atcccatcca gcttcacatt
     7801 tcaattagag aggcagactt cttttccagt aatattgtac acctagtgtt gctatgagta
     7861 aaatcagtag gaaaaatact gagaaggtct gggaagacgc actgactagg ccagcggtca
     7921 catttgtaaa tgtacctttc ccataccttc cagtggaggc attcagctgc gacttttcag
     7981 accctaaaac caagccaggg aggaggaaca catgtttaag gttagagaag gccaggagtg
     8041 cgggtgcata ccctgaggca aaggccatcc tttgctagat agagaacctg agatgctgag
     8101 acccattggt gaaccagtgt ggtagtgcat gctttaattc tagcacttgg actgtcagtt
     8161 tgagaccagc atgctctaca cagagagacc ctgggagaaa gcaaagtgta gtaagaggat
     8221 gccattctgc agaactcgtg ttaattacat gaaccccatg gttaactaaa aagaacttac
     8281 tgttcccaga gccagggaat agaaaagaag ctacacttct cttgtaaatg gtatttagaa
     8341 caaggtgttc gggtaaagtc tcaactagtc agaggtttca gcagcttaaa ttgctaatat
     8401 ttaagaagtt gattttcagg aatgtctgcc tgtgcctctc tccttgctgc cattgatcag
     8461 ctcagcagga atcatcatct ttaaacgtgg agaaagctct cagatgagcg tggacaagct
     8521 cccacccaga agtcctaatg atacttggtt ttgactgtgc tgcttctgtg ttaggtttct
     8581 tctcagcttt tagccactgg ggttttctgc ctgttctgta cctctctaga agatgtcgac
     8641 ttggaagtgc tgcctttcac accagtgcca tggtgtccat atagttgagc ttgttgctga
     8701 tccagaggtg tcccctttat gctcacacct ggcacatcac tgacatgagc attcaacagt
     8761 acatgggtga gggtagaggt ggggctagcc aacagccctg gctcaattgg cagggcctgc
     8821 aaaggcccca cttgagggcc agacactagg ttggtaagca cagttaggga aatttctacc
     8881 taacctccgt tttgaatgat tctgagaaat tttatatatg gaggatggaa atgcttatat
     8941 atttattgaa gatcttttta tttgaaatta aaatcatttg gtaaataatg gtttgatatt
     9001 tggggagggt tttggaggga agtaacatgg ttgataaatt acaggcctga tgactcccct
     9061 gaagggcgct gttgctgatg gagagagagg cctttatctt gtacatgatc caatctttgt
     9121 cttaggtttc ttttatccaa aatatctagg catctgacac tttcaaccaa aatttcaatt
     9181 atatactgtg atttttattt gttgcaatac gttaaatttc agaggtactt tggggctcaa
     9241 gagctaggat ttctaagtca gtatgtgcat ttcacataat aagctgttaa tggtgagcaa
     9301 agtattatat actaactaga ttttccacat ctgtatagta tattatatgt attttgtggt
     9361 attgagatta tagaaagttt agagtgtcaa acatgcgttt aatgtgtatt tttaaacaaa
     9421 tttatggcaa ttaaaatatt tggagacaag ggtatcacat ttcaatttgt aaaactcttt
     9481 ttaactctac tgtgtcatta caatgctttg tataatgcca aaccatagct ggagaaacta
     9541 agtttaataa atatcagcta gcttttctgt actacgtttg catgcactgt gctctcgctt
     9601 cttcagctgc tgctgtgatc tcttgtgtag ttgctctttg tgtcttaggg ttcagtctga
     9661 ctctgcaaag gtctggcccc gccagcacag atctcagttt ttggttacag aaattttgga
     9721 ttctggactg aatagatgac ttagaagtta agggccactg cctgcacttc cagatgaccc
     9781 aggttcaagt ctcagcaacc atatggtggc tcacagctgt ctgtgactac agttgcccgg
     9841 gttctaacac ccttacacag acatgaatac agacaaagca cataaagtaa atttttaaaa
     9901 aaaattttgg gtgcatggcc atgggtccgt gtccgtgagt ctcagttatc ctgcaccaac
     9961 agacatcttt aatcttttct tgatgctctt aagacataca ctattaaaga aaaaaaaaag
    10021 aatgtacact attatttatt tttactattt atgtattggt tctttttatt cttgtttttc
    10081 tgagacaggg tttcattatg tagccctggc tagtctggaa ctcactgtat atacctggtt
    10141 gtccttgaac ttacagagat tggcc
//
