LOCUS       NM_001080798            8412 bp    mRNA    linear   ROD 27-JUL-2024
DEFINITION  Mus musculus AF4/FMR2 family, member 1 (Aff1), transcript variant
            1, mRNA.
ACCESSION   NM_001080798
VERSION     NM_001080798.3
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 8412)
  AUTHORS   Tamai,H., Yamaguchi,H., Miyake,K., Takatori,M., Kitano,T.,
            Yamanaka,S., Yui,S., Fukunaga,K., Nakayama,K. and Inokuchi,K.
  TITLE     Amlexanox Downregulates S100A6 to Sensitize KMT2A/AFF1-Positive
            Acute Lymphoblastic Leukemia to TNFalpha Treatment
  JOURNAL   Cancer Res 77 (16), 4426-4433 (2017)
   PUBMED   28646023
REFERENCE   2  (bases 1 to 8412)
  AUTHORS   Okuda,H., Stanojevic,B., Kanai,A., Kawamura,T., Takahashi,S.,
            Matsui,H., Takaori-Kondo,A. and Yokoyama,A.
  TITLE     Cooperative gene activation by AF4 and DOT1L drives MLL-rearranged
            leukemia
  JOURNAL   J Clin Invest 127 (5), 1918-1931 (2017)
   PUBMED   28394257
  REMARK    GeneRIF: These results reveal a cooperative transcriptional
            activation mechanism of AEP and DOT1L and suggest a molecular
            rationale for the simultaneous inhibition of the MLL fusion-AF4
            complex and DOT1L for more effective treatment of MLL-rearranged
            leukemia.
REFERENCE   3  (bases 1 to 8412)
  AUTHORS   Barrett,N.A., Malouf,C., Kapeni,C., Bacon,W.A., Giotopoulos,G.,
            Jacobsen,S.E.W., Huntly,B.J. and Ottersbach,K.
  TITLE     Mll-AF4 Confers Enhanced Self-Renewal and Lymphoid Potential during
            a Restricted Window in Development
  JOURNAL   Cell Rep 16 (4), 1039-1054 (2016)
   PUBMED   27396339
REFERENCE   4  (bases 1 to 8412)
  AUTHORS   Oliver,P.L., Bitoun,E., Clark,J., Jones,E.L. and Davies,K.E.
  TITLE     Mediation of Af4 protein function in the cerebellum by Siah
            proteins
  JOURNAL   Proc Natl Acad Sci U S A 101 (41), 14901-14906 (2004)
   PUBMED   15459319
REFERENCE   5  (bases 1 to 8412)
  AUTHORS   Isaacs,A.M., Oliver,P.L., Jones,E.L., Jeans,A., Potter,A.,
            Hovik,B.H., Nolan,P.M., Vizor,L., Glenister,P., Simon,A.K.,
            Gray,I.C., Spurr,N.K., Brown,S.D., Hunter,A.J. and Davies,K.E.
  TITLE     A mutation in Af4 is predicted to cause cerebellar ataxia and
            cataracts in the robotic mouse
  JOURNAL   J Neurosci 23 (5), 1631-1637 (2003)
   PUBMED   12629167
  REMARK    GeneRIF: demonstrated that Af4 is specifically expressed in
            Purkinje cells; hypothesize that the expression of mutant Af4 leads
            to neurodegeneration
REFERENCE   6  (bases 1 to 8412)
  AUTHORS   Isnard,P., Core,N., Naquet,P. and Djabali,M.
  TITLE     Altered lymphoid development in mice deficient for the mAF4
            proto-oncogene
  JOURNAL   Blood 96 (2), 705-710 (2000)
   PUBMED   10887138
REFERENCE   7  (bases 1 to 8412)
  AUTHORS   Taki,T., Kano,H., Taniwaki,M., Sako,M., Yanagisawa,M. and
            Hayashi,Y.
  TITLE     AF5q31, a newly identified AF4-related gene, is fused to MLL in
            infant acute lymphoblastic leukemia with ins(5;11)(q31;q13q23)
  JOURNAL   Proc Natl Acad Sci U S A 96 (25), 14535-14540 (1999)
   PUBMED   10588740
REFERENCE   8  (bases 1 to 8412)
  AUTHORS   Isnard,P., Depetris,D., Mattei,M.G., Ferrier,P. and Djabali,M.
  TITLE     cDNA cloning, expression and chromosomal localization of the murine
            AF-4 gene involved in human leukemia
  JOURNAL   Mamm Genome 9 (12), 1065-1068 (1998)
   PUBMED   9880680
REFERENCE   9  (bases 1 to 8412)
  AUTHORS   Baskaran,K., Erfurth,F., Taborn,G., Copeland,N.G., Gilbert,D.J.,
            Jenkins,N.A., Iannaccone,P.M. and Domer,P.H.
  TITLE     Cloning and developmental expression of the murine homolog of the
            acute leukemia proto-oncogene AF4
  JOURNAL   Oncogene 15 (16), 1967-1978 (1997)
   PUBMED   9365243
REFERENCE   10 (bases 1 to 8412)
  AUTHORS   Gu,Y., Nakamura,T., Alder,H., Prasad,R., Canaani,O., Cimino,G.,
            Croce,C.M. and Canaani,E.
  TITLE     The t(4;11) chromosome translocation of human acute leukemias fuses
            the ALL-1 gene, related to Drosophila trithorax, to the AF-4 gene
  JOURNAL   Cell 71 (4), 701-708 (1992)
   PUBMED   1423625
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL713989.20 and AL731554.13.
            
            On Jun 14, 2022 this sequence version replaced NM_001080798.2.
            
            Summary: This gene encodes a member of the AF4/ lymphoid nuclear
            protein related to the Fragile X E syndrome (FRAXE) family of
            proteins, which have been implicated in human childhood
            lymphoblastic leukemia, fragile chromosome X intellectual
            disability, and ataxia. It is the prevalent mixed-lineage leukemia
            fusion gene associated with spontaneous acute lymphoblastic
            leukemia. Members of this family have three conserved domains: an
            N-terminal homology domain, an AF4/ lymphoid nuclear protein
            domain, and a C-terminal homology domain. Knockout of the mouse
            gene by homologous recombination severely affects early events in
            lymphopoiesis, including precursor proliferation or recruitment,
            but is dispensable for terminal differentiation. In addition, an
            autosomal dominant missense mutation results in several phenotypes
            including ataxia and adult-onset Purkinje cell loss in the
            cerebellum, indicating a role in Purkinje cell maintenance and
            function. Alternative splicing results in multiple transcript
            variants. [provided by RefSeq, Jul 2017].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMN00849377,
                              SAMN00849381 [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-273               AL713989.20        13541-13813         c
            274-394             AL731554.13        142242-142362       c
            395-1252            AL731554.13        140973-141830       c
            1253-1297           AL731554.13        114379-114423       c
            1298-1384           AL731554.13        110418-110504       c
            1385-1421           AL731554.13        109237-109273       c
            1422-1473           AL731554.13        106500-106551       c
            1474-1528           AL731554.13        99452-99506         c
            1529-1566           AL731554.13        99306-99343         c
            1567-1729           AL731554.13        96976-97138         c
            1730-2719           AL731554.13        91027-92016         c
            2720-2817           AL731554.13        84333-84430         c
            2818-3058           AL731554.13        83019-83259         c
            3059-3159           AL731554.13        82398-82498         c
            3160-3220           AL731554.13        82218-82278         c
            3221-3354           AL731554.13        79119-79252         c
            3355-3426           AL731554.13        78379-78450         c
            3427-3564           AL731554.13        77635-77772         c
            3565-3791           AL731554.13        75910-76136         c
            3792-8412           AL731554.13        70164-74784         c
FEATURES             Location/Qualifiers
     source          1..8412
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 50.45 cM"
     gene            1..8412
                     /gene="Aff1"
                     /gene_synonym="9630032B01Rik; Af4; Mllt2h; Rob"
                     /note="AF4/FMR2 family, member 1"
                     /db_xref="GeneID:17355"
                     /db_xref="MGI:MGI:1100819"
     exon            1..273
                     /gene="Aff1"
                     /gene_synonym="9630032B01Rik; Af4; Mllt2h; Rob"
                     /inference="alignment:Splign:2.1.0"
     exon            274..394
                     /gene="Aff1"
                     /gene_synonym="9630032B01Rik; Af4; Mllt2h; Rob"
                     /inference="alignment:Splign:2.1.0"
     exon            395..1252
                     /gene="Aff1"
                     /gene_synonym="9630032B01Rik; Af4; Mllt2h; Rob"
                     /inference="alignment:Splign:2.1.0"
     exon            1253..1297
                     /gene="Aff1"
                     /gene_synonym="9630032B01Rik; Af4; Mllt2h; Rob"
                     /inference="alignment:Splign:2.1.0"
     exon            1298..1384
                     /gene="Aff1"
                     /gene_synonym="9630032B01Rik; Af4; Mllt2h; Rob"
                     /inference="alignment:Splign:2.1.0"
     exon            1385..1421
                     /gene="Aff1"
                     /gene_synonym="9630032B01Rik; Af4; Mllt2h; Rob"
                     /inference="alignment:Splign:2.1.0"
     exon            1422..1473
                     /gene="Aff1"
                     /gene_synonym="9630032B01Rik; Af4; Mllt2h; Rob"
                     /inference="alignment:Splign:2.1.0"
     exon            1474..1528
                     /gene="Aff1"
                     /gene_synonym="9630032B01Rik; Af4; Mllt2h; Rob"
                     /inference="alignment:Splign:2.1.0"
     exon            1529..1566
                     /gene="Aff1"
                     /gene_synonym="9630032B01Rik; Af4; Mllt2h; Rob"
                     /inference="alignment:Splign:2.1.0"
     exon            1567..1729
                     /gene="Aff1"
                     /gene_synonym="9630032B01Rik; Af4; Mllt2h; Rob"
                     /inference="alignment:Splign:2.1.0"
     exon            1730..2719
                     /gene="Aff1"
                     /gene_synonym="9630032B01Rik; Af4; Mllt2h; Rob"
                     /inference="alignment:Splign:2.1.0"
     exon            2720..2817
                     /gene="Aff1"
                     /gene_synonym="9630032B01Rik; Af4; Mllt2h; Rob"
                     /inference="alignment:Splign:2.1.0"
     exon            2818..3058
                     /gene="Aff1"
                     /gene_synonym="9630032B01Rik; Af4; Mllt2h; Rob"
                     /inference="alignment:Splign:2.1.0"
     exon            3059..3159
                     /gene="Aff1"
                     /gene_synonym="9630032B01Rik; Af4; Mllt2h; Rob"
                     /inference="alignment:Splign:2.1.0"
     exon            3160..3220
                     /gene="Aff1"
                     /gene_synonym="9630032B01Rik; Af4; Mllt2h; Rob"
                     /inference="alignment:Splign:2.1.0"
     exon            3221..3354
                     /gene="Aff1"
                     /gene_synonym="9630032B01Rik; Af4; Mllt2h; Rob"
                     /inference="alignment:Splign:2.1.0"
     exon            3355..3426
                     /gene="Aff1"
                     /gene_synonym="9630032B01Rik; Af4; Mllt2h; Rob"
                     /inference="alignment:Splign:2.1.0"
     exon            3427..3564
                     /gene="Aff1"
                     /gene_synonym="9630032B01Rik; Af4; Mllt2h; Rob"
                     /inference="alignment:Splign:2.1.0"
     exon            3565..3791
                     /gene="Aff1"
                     /gene_synonym="9630032B01Rik; Af4; Mllt2h; Rob"
                     /inference="alignment:Splign:2.1.0"
     exon            3792..8412
                     /gene="Aff1"
                     /gene_synonym="9630032B01Rik; Af4; Mllt2h; Rob"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7918..7923
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Aff1"
                     /gene_synonym="9630032B01Rik; Af4; Mllt2h; Rob"
                     /note="hexamer: ATTAAA"
     polyA_site      7939
                     /gene="Aff1"
                     /gene_synonym="9630032B01Rik; Af4; Mllt2h; Rob"
                     /note="major polyA site"
     regulatory      8387..8392
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Aff1"
                     /gene_synonym="9630032B01Rik; Af4; Mllt2h; Rob"
                     /note="hexamer: AATAAA"
     polyA_site      8412
                     /gene="Aff1"
                     /gene_synonym="9630032B01Rik; Af4; Mllt2h; Rob"
                     /note="major polyA site"
ORIGIN      
        1 atctcatccc tgagtgtcgg gtagaaaagt gtgtgaaccg agtgcaaagg gaccgcccgg
       61 aaagttaagg ttaaagattc cgcttctggc ccgaagaact gttattttcg gaaagtgaag
      121 ttggtttctc tcctgccgga gcctggattt tcactgaggt gcctgtgcac aatcctccgg
      181 ctctactcgt tgctggtttg gcgtcttcca aagaaatttg gaagcgcact taatggaaac
      241 gaggattctc ccacgagaga gtggtggagc ctgcttgtac aatgaagata gaaacctgct
      301 tcgaatcaga gagaaggaaa gacgcaacca agaagctcac caggagaagg aggcatttcc
      361 cgagaaggct cccctgtttc cagagcctta caagactgca aaaggcgatg agctatcaag
      421 tcggatccag accatgctgg gtgactatga ggagatgaag gagttcctga gcagcaagtc
      481 ccacccccac cgcctggatg gctctgaaga caggcccggg aagcccagat atcccttagg
      541 tcatgacagg gggaacgggg ctgcatccag ctccctccgc acacatgtct accaccagcc
      601 tatccacact tctgctcccg gatcacgtcc tgtcggtaac attagccaca gtccaaagat
      661 ggcacagcca aggatggagc caagtctcca caccaaaatc tatgatggcc cacgtctgac
      721 tcaagaccac ctcagtcagg gacattgttc cagaaagtgt gaccgaagag ctgaaggaga
      781 ctctgctccc gagaggaagc tctcgccctt gatctcctct ttgccatccc cggtgccccc
      841 tctatcacct gtacattcca ggctgcaggg aaccagcaag gctcacagca gtggcgttag
      901 cagtaaaagc tgctgtgtgg ccaagtcttc caaggacttg gtggcgaagg cccaagataa
      961 agagactcct catgacggtt tggtggcagt taccagcctt ggatcagccc ctcctcagcc
     1021 accttgccag acatttccac cccctcctct cccctcaaaa agtgctgcaa tgcagcagaa
     1081 gcccacggca tatgtccgtc ccatggacgg tcaggatcag gctcccagtg agtcccccga
     1141 gctgaagctg ccactggagg actatggtca gcaaagcttt gagaaaccag accttaaagt
     1201 gcctgccaaa gccaagctca ccagactaag gatgccctct cagtcggtgg agcagccgta
     1261 ctccaatgaa gtccattgcg ttgaagagat tctgaaggaa atgacccact cgtggccacc
     1321 gcctctgaca gccatacaca cacccagtac agccgagccg tccagatttc ctttccccac
     1381 aaaggaccct ctgcacgtca gtcctgcaac ccagagccaa aaacaatatg acacgccttc
     1441 aaaaacccac cccaatcccc agcaaggaac ctccatgctg gaggacgacc tgcagctcag
     1501 cgacagtgaa gacagcgaca cggagcaagc cacagagaag cctccatccc cacctgcacc
     1561 tcccagcgct ccgcagacac ttcccgagcc cgtggcctca gcacattcca gtagtgggga
     1621 gtcagagagc agcgagtcag acagttcctc agactctgag agtgagagca gctccagcga
     1681 cagcgaggag gaggaagaga acgagcccct ggagacccgg gctcccgagc ccgagcctcc
     1741 gacgacaaac aaatggcagc tggacaactg gttgaccaaa gtcaaccaac cctcagtgcc
     1801 cctggatggc cgggggagca cagagtctcc acaatggcgc caggaaagta agggtgtcgc
     1861 tgagggcagc agtgaccagc agcatcctga ttccaaagac cctctcccaa agagctccag
     1921 caagactctc cggggtccct ctgaggggcc ccagcctggg aagaggggct gtccgaaatc
     1981 ccccgctcaa caggaacccc cacctcggca aaccgtgggc agcaaacaac ccaggaaacc
     2041 cgccaagggc tccggccagg cagagcccca ggccagctcg caggcagaga gtgaagtagg
     2101 accccttccc tacggctcaa aggagcagac ttccaaagac agacccaagg tgaagacgaa
     2161 aggcaggccg cgtgccgtgg gcagtcggga gcctaagcca gaggtccctg cgcccacgcc
     2221 tcaggcagcc gtgcccagac cgaagccgcc tgtgcccacc cccagtgaga agaggaagca
     2281 caagagctcc acggccccct ccaaggcgcc ctcagccccc cagcctccga aggacagtgc
     2341 tggggacagg aacccagaac actctgctct tgtctccttg actcagagcc agggtccatc
     2401 ccacagcagc aggggcagca gtggcagcgt caggactagt ggctgccgac aggctgtgat
     2461 tgcccaaggg gacggctgta aagacaagct cctgttgcct ttgagagaca ccaaattgct
     2521 ctcgccgctc agggactccc caccaccgac aagcttggtg gtgaagataa ccctagacct
     2581 cctcacgagg attccccagc ctctggggaa ggggagccgc cccaggaaag cagaagacaa
     2641 gcagctgtca gcagggaaga agcaggactc ggagaccaag agctgtgaca gctccagcag
     2701 agtgaccaag aagagaaagg gtgacccaga aaaagagcac agtaacaaga gacacaaact
     2761 ggataagtcg cagactgcgt catcttcatc ttcccataca gaatcttcca gaacaaaagc
     2821 gcccaggtcc tcctcagaga actcaagaaa ggaaatgctg cctccagcat cggcgtcctc
     2881 agtctcctct tcctcatcct cccagaagcc aagcagacct gcacagaaga ggccaaggcc
     2941 agacgaagac acgtgtagcc aggaaccccc cagaagtgcc agcagcacaa agagcagctc
     3001 tacagaccct cccgctccca agcacagaaa agtacaggcc aggggctctg aacacaaggg
     3061 atcttctggg gatgctgcga atgctgcaaa tccttttcca gtgccttctt tgccgaatgg
     3121 taacgctaaa ccagggaagc cacaggtgaa gtctgacaga caacaagctg actttcacat
     3181 gaaggaggct aaaaagctga agtgcaaagc agagacaatg gtagacaagg ctggaaaagc
     3241 cttcaagtat ttggaagccg tcctctcttt tatcgagtgt ggaatggcct cagagtcaga
     3301 aagctcagcc aagtcggcgt acgctgtgta ctcagaaacc atagacctca ttaggtatgt
     3361 gatgtcacta aaatgcttct cagataacac gatgccagca caagagaaga tatttgctgt
     3421 tttatgcttg cgttgccagt cccttttgaa catggcgatg tttcgatgta agaaagacac
     3481 agtgatgaag tattctcgta ctctcagtga acacttcaag agtacttcca aagtggccca
     3541 ggcaccttct ccatgcacgg caagaagcac aggcgttccg tcccccctct ccccaatgcc
     3601 ttctcctgcc agctccgtgg ggtcccaatc aagtgctggc agcagtatgg ggagcgtcgg
     3661 ggtgacggcc accgtcagta ctccagtctc catccagaac atgacctcct cctatgtcac
     3721 catcacatcc catgtcctta cggcctttag cctttgggaa caggccgagg ccctcacaag
     3781 gaagaacaaa gaattcttcg ctcagctcag cacaaaagtg cgtgtgttgg ccctcaacag
     3841 cagcctggtg gacctggtgc actacacaag acagggcctg cagcggctaa aacagtcacc
     3901 caaaggcccc taacgtaggc cggacctact cctgcctcgg agtgtctctg caccattgga
     3961 agccatgatc cagtgcagac aacgactgtc agatcggaat gtcagtactc aacaagagaa
     4021 gcacccgaga gatggccggg atgcttgtcc acttaaactc acaacagctg tgtggtcatc
     4081 gctcagcgct ggtcatctga gcagatgctg caagatggcc ttgcctttgc caaccggagg
     4141 acaagtacaa cggagcccca ctggcgtatg aatggtctag aaacatttct ggtttttttc
     4201 tgtttgtttg ggcgatagga taaaaattgc aaatgagagg aaaatttcag caaactcttg
     4261 tcatctcaga agccatccta gtttgcatcc ggaaggaatt ttttttttaa ataataataa
     4321 tttctggtga agggctttaa agattttgtt ttcttttcaa gttttaaaga atacattgtt
     4381 tgatacattc tgatggccat ctgtggaaca caccttgtga aggactccat tgcactcccc
     4441 ttactgcagg gaaggggctc tgatgacaga gtactgatat ttacagaatt gaaggtgaca
     4501 accccactcc ccagtgccta gagtgctgca cattgtctag ctctggtctc tctggctgtc
     4561 ctgagaagaa tccctagtca gtcctatggg ggagtggcag catttggatg cgggttctat
     4621 ctcacagtct tccccacaac cctagaggcc taacacttcc tcctgacaca cgccaccaaa
     4681 gggactggct aagcagcatt gaccttgggc gaacacagga gcctcatgtt gactttgggc
     4741 tgtaggcaga tgcagttcat catctgtctg ctggagctgg agtcccgggc actgacagtg
     4801 ctgtggctac cctgtgtatc agtgagcgaa agtgagaagt cttcctagag aggtattttt
     4861 ctgctacaag tgttttatat ttatagcaaa actcagcttt ctatccagga tatccttggc
     4921 tatccagggg acattagctc ctggagagga tgtggagttt gggggggggg gcaggattaa
     4981 cttcaacttt aaaaaacacc ccactatcat acaccttcag tccagaccat cttctaaggt
     5041 atcgtgactt gatgaggttg aagaagatct gtgaccttcc caggctacac cccacccaca
     5101 gtagaccagg ttcacttaga tgggaagtga agaatacact cagaattcac acctgcccgt
     5161 aaacacaggc ctttccttga gacttcaagg ttactactgg aatactctca agtgttttta
     5221 aaaagccagt tctttttaat cccttctaga aagaaagtag aatttgcaca tgttcaccat
     5281 ggaggtgtgt tacggctccc cctgcctctc tttcctgcca gccatgcttg tctgtgtgca
     5341 tttggagatt agtggagatg ggagacccat ctttccttag tgatgatgtc tgtggtcctt
     5401 gtggtaacat caggactttc ttggtacctt ggttaaatct acacagggtc tctaggtgag
     5461 tgcgatacca ggtttttcag ggcaaactgt ctgtgatact ccttggtctt ctcagccttc
     5521 tctcctggtt tgaatgaagc cagaaaatgg ctaccttgta catatctgag tatagaccaa
     5581 gacggtgatc cagctaagcc cttttcttta ttgatattct gaagccaagc catgtctgac
     5641 tgagtcgaag tgtatggtag gtggtcccta gcacagtggg gcttgtgggc tcatcaggat
     5701 cttttctccc acagccctgc atcccttgca gcctgttggt tgtaaaatgg ctatgatcct
     5761 tgtgagaggt tctggcctac tgaagttttg ccagagcatg tgtacatcct gccagcagat
     5821 cactgtgccc cttgcctgga cagaatcagc cagtgtagga agaacaggct gcctcattct
     5881 ttaatgggtg ggttttctcc tcagttttat ttgtaccttg aaaaccaatg cagttggaca
     5941 ggatccgttt gtgccagttt ggtctgtagc tattgcactg ctggttttcc cacaacactg
     6001 cacctagaga gcatgagcct tgtctgtctt cttcagggga aaaccccagg gctgttctgc
     6061 gctgcttatt gtaaaacccc gaagactcaa attttatacc atctgcacag ttcactgtgt
     6121 gcttaaactt accagaggca aattttcccc tgaggtgatt attttgggag gactataaca
     6181 ttagcccctt tgaatatctg atgttcttaa aaccatggaa gagcatccac cttatggggg
     6241 ggggcagcat ttaatgaatg aatcttccat ttgccactac aaagggtgtg tgtggcccat
     6301 ctgtctggga cacaaagtag aggaacacaa taatgttttc tcttttggaa aaaattaaaa
     6361 ttagatacat gtaaaaaacc ctgttactaa aacaggttgc ccttggttaa ggtgcctaga
     6421 gggcagacca catggcagta gggaggagca ggagggccca gcaagtgctt gctaatggtc
     6481 ccttacaaag tgacatttat tttgtagagg ttctgacaaa tgtctatcca ttccctggac
     6541 caccaccacc cagcctcatc tgtaggaagt ttcaaacggg ctcttcccca tcttaattga
     6601 aaatgtgcct ttactatgtt gatgttaggg acaggacctt aagtcctacg attcctgttt
     6661 ggtaacttat aaaaggactt gtgctcttga gctgtggtct ctgatgcaga agatgagaga
     6721 ttcagctaga cgtatttgtg cctttgctga ccaaacataa attctgaagt tacgctgttt
     6781 gttttgctgt ggctttgagt gggttcatgg gttttatatg tttcctttcc tgctttgtga
     6841 aattcgagat gagttgggct taaaaaatat tatatatatc taagatcttt ccatacctct
     6901 tacagattct ttttcttctt aatgacttat ttcccccaac actacgaaat gtctttgaaa
     6961 atttaccact tcatctgtta ccttcttcat caatggttag tatttaaatc gcctcagaat
     7021 gttttagggg ctcctgtcta aaacacagaa gtgtaggttt ttctcattga agaatgagct
     7081 atggagatgc gaggatcaat tttgatgccc ccctccccct gcctctttcc cctggttttg
     7141 gctcatttgg aactactggg caagaggact tttcctgtgg aagggaagtg taaggacaga
     7201 cgggcatgtg ttgggatttt gggatttctg ccaggcgatc ctccatatgc agtccagttt
     7261 cccacttact gagagcagct gtctggtagc cgtgcctctt actgatgcat tgacaagcac
     7321 cgcatcacca catttctttg agcgactgta aaatttcctg gttccttggg ggtgaatcac
     7381 actctgttct gttccgctgt gcgtcagaca tcagcagtta ctgtggtggg cagagaagtg
     7441 cctctttccc tacctaagag ggagccagag gacaggaagg aagcgtagag ctctctgcaa
     7501 gcctgtatat tgctatattt gtccccatag gacaccggat tgaggctggc ggcaggcaag
     7561 ccgggctgca ctgttgagct ggggcggggg gaggggggca gaggctgacc taaggaagat
     7621 gaactcactt tgcttagatg ttttagcaat gctcagtaca ggcagtatta cgtttgcctt
     7681 tcattcaggc catgacatgt attgctaaga acactgcaca tctcatgata tcgcgggtac
     7741 actgttcgtg gtggggtgtg tccatgtgtg tcccgaattt taattttttt ctgaatgtga
     7801 tcatgctttg gatgatacct gagcagggtt gccttttttt tttaatttat taccattata
     7861 tattatatta tatattatat attttctttc ttaaaatgtt agaagtcaaa tcttggtatt
     7921 aaaattgctt taaaaaggag tatgttgtca aggtgataat gagaatcact ggtctgcctt
     7981 taatgagttt ttctttatca tggaataatg tgccagctct gatcagcaca gtggttttgt
     8041 gtgcaggctg gggaagcagc caggctcccg gtgaagatgc gcagtgcaaa tacggggagc
     8101 accacagatg taaaaatcag catcagcagt ggaacggcac ccctggggct tctgtgaatg
     8161 gggttcatct gcctcatggg agactctgaa agggggcaga cgtgatctgt gagagttttt
     8221 tttaaaagaa actgcaaaag atctttccaa agatgatgtg ccacagatct tttgtttttc
     8281 tctgtaacaa ggactaagtg ctgtcttaga aaaggtaatt gacttgttca tcttcacaat
     8341 ctttcctttc cacaagatca aactgtaggg aaaaaaaagg atatttaata aaaatttaga
     8401 gaaatgattg ga
//
