LOCUS       NM_001008533            5837 bp    mRNA    linear   ROD 09-FEB-2025
DEFINITION  Mus musculus adenosine A1 receptor (Adora1), transcript variant 2,
            mRNA.
ACCESSION   NM_001008533
VERSION     NM_001008533.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5837)
  AUTHORS   Tian,T., Yu,Q., Yang,D., Zhang,X., Zhang,C., Li,J., Luo,T.,
            Zhang,K., Lv,X., Wang,Y., Wang,H. and Li,H.
  TITLE     Endothelial alpha1-adrenergic receptor activation improves cardiac
            function in septic mice via PKC-ERK/p38MAPK signaling pathway
  JOURNAL   Int Immunopharmacol 141, 112937 (2024)
   PUBMED   39182270
  REMARK    GeneRIF: Endothelial alpha1-adrenergic receptor activation improves
            cardiac function in septic mice via PKC-ERK/p38MAPK signaling
            pathway.
REFERENCE   2  (bases 1 to 5837)
  AUTHORS   Stekic,A., Stevic,D., Dokmanovic,T., Anastasov,M., Popovic,D.,
            Stanojevic,J., Jovanovic,M.Z., Stevanovic,I., Nedeljkovic,N. and
            Dragic,M.
  TITLE     Intrinsic ecto-5'-Nucleotidase/A1R Coupling may Confer
            Neuroprotection to the Cerebellum in Experimental Autoimmune
            Encephalomyelitis
  JOURNAL   Mol Neurobiol 61 (11), 9284-9301 (2024)
   PUBMED   38619745
  REMARK    GeneRIF: Intrinsic ecto-5'-Nucleotidase/A1R Coupling may Confer
            Neuroprotection to the Cerebellum in Experimental Autoimmune
            Encephalomyelitis.
REFERENCE   3  (bases 1 to 5837)
  AUTHORS   Cui,X., Wei,W., Zhang,Z., Liu,K., Zhao,T., Zhang,J., Zheng,A.,
            Xi,H., He,X., Wang,S., Zhu,B. and Gao,X.
  TITLE     Caffeine Impaired Acupuncture Analgesia in Inflammatory Pain by
            Blocking Adenosine A1 Receptor
  JOURNAL   J Pain 25 (4), 1024-1038 (2024)
   PUBMED   37918469
REFERENCE   4  (bases 1 to 5837)
  AUTHORS   Zhu,W., Hong,Y., Tong,Z., He,X., Li,Y., Wang,H., Gao,X., Song,P.,
            Zhang,X., Wu,X., Tan,Z., Huang,W., Liu,Z., Bao,Y., Ma,J., Zheng,N.,
            Xie,C., Ke,X., Zhou,W., Jia,W., Li,M., Zhong,J., Sheng,L. and Li,H.
  TITLE     Activation of hepatic adenosine A1 receptor ameliorates MASH via
            inhibiting SREBPs maturation
  JOURNAL   Cell Rep Med 5 (3), 101477 (2024)
   PUBMED   38508143
  REMARK    GeneRIF: Activation of hepatic adenosine A1 receptor ameliorates
            MASH via inhibiting SREBPs maturation.
            Erratum:[Cell Rep Med. 2024 May 21;5(5):101563. doi:
            10.1016/j.xcrm.2024.101563.. PMID: 38718795]
REFERENCE   5  (bases 1 to 5837)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   6  (bases 1 to 5837)
  AUTHORS   Tatsis-Kotsidis,I. and Erlanger,B.F.
  TITLE     A1 adenosine receptor of human and mouse adipose tissues: cloning,
            expression, and characterization
  JOURNAL   Biochem Pharmacol 58 (8), 1269-1277 (1999)
   PUBMED   10487528
REFERENCE   7  (bases 1 to 5837)
  AUTHORS   Blackburn,M.R., Wubah,J.A., Chunn,J.L., Thompson,L.F. and
            Knudsen,T.B.
  TITLE     Transitory expression of the A2b adenosine receptor during
            implantation chamber development
  JOURNAL   Dev Dyn 216 (2), 127-136 (1999)
   PUBMED   10536053
REFERENCE   8  (bases 1 to 5837)
  AUTHORS   Rivkees,S.A., Chen,M., Kulkarni,J., Browne,J. and Zhao,Z.
  TITLE     Characterization of the murine A1 adenosine receptor promoter,
            potent regulation by GATA-4 and Nkx2.5
  JOURNAL   J Biol Chem 274 (20), 14204-14209 (1999)
   PUBMED   10318839
REFERENCE   9  (bases 1 to 5837)
  AUTHORS   Borglum,J.D., Vassaux,G., Richelsen,B., Gaillard,D., Darimont,C.,
            Ailhaud,G. and Negrel,R.
  TITLE     Changes in adenosine A1- and A2-receptor expression during adipose
            cell differentiation
  JOURNAL   Mol Cell Endocrinol 117 (1), 17-25 (1996)
   PUBMED   8734470
REFERENCE   10 (bases 1 to 5837)
  AUTHORS   Marquardt,D.L., Walker,L.L. and Heinemann,S.
  TITLE     Cloning of two adenosine receptor subtypes from mouse bone
            marrow-derived mast cells
  JOURNAL   J Immunol 152 (9), 4508-4515 (1994)
   PUBMED   8157966
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC137104.11, BY257408.1, AK045729.1 and DV552876.1.
            
            On Sep 24, 2013 this sequence version replaced NM_001008533.2.
            
            Transcript Variant: This variant (2) represents the longest
            transcript and encodes the longest isoform (a). Variants 1, 2, and
            3 encode the same protein.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK043954.1, AK134709.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-30                AC137104.11        21172-21201
            31-461              BY257408.1         9-439
            462-3430            AK045729.1         2-2970
            3431-5732           AC137104.11        55026-57327
            5733-5837           DV552876.1         138-242
FEATURES             Location/Qualifiers
     source          1..5837
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 58.15 cM"
     gene            1..5837
                     /gene="Adora1"
                     /gene_synonym="A1-AR; A1AR; A1R; AA1R; ARA1; Ri"
                     /note="adenosine A1 receptor"
                     /db_xref="GeneID:11539"
                     /db_xref="MGI:MGI:99401"
     exon            1..1443
                     /gene="Adora1"
                     /gene_synonym="A1-AR; A1AR; A1R; AA1R; ARA1; Ri"
                     /inference="alignment:Splign:2.1.0"
     CDS             1103..2083
                     /gene="Adora1"
                     /gene_synonym="A1-AR; A1AR; A1R; AA1R; ARA1; Ri"
                     /note="isoform a is encoded by transcript variant 2;
                     adenosine receptor A1"
                     /codon_start=1
                     /product="adenosine receptor A1 isoform a"
                     /protein_id="NP_001008533.1"
                     /db_xref="CCDS:CCDS15305.1"
                     /db_xref="GeneID:11539"
                     /db_xref="MGI:MGI:99401"
                     /translation="MPPYISAFQAAYIGIEVLIALVSVPGNVLVIWAVKVNQALRDAT
                     FCFIVSLAVADVAVGALVIPLAILINIGPQTYFHTCLMVACPVLILTQSSILALLAIA
                     VDRYLRVKIPLRYKTVVTQRRAAVAIAGCWILSLVVGLTPMFGWNNLSEVEQAWIANG
                     SVGEPVIKCEFEKVISMEYMVYFNFFVWVLPPLLLMVLIYLEVFYLIRKQLNKKVSAS
                     SGDPQKYYGKELKIAKSLALILFLFALSWLPLHILNCITLFCPTCQKPSILIYIAIFL
                     THGNSAMNPIVYAFRIHKFRVTFLKIWNDHFRCQPKPPIEEDIPEEKADD"
     exon            1444..5819
                     /gene="Adora1"
                     /gene_synonym="A1-AR; A1AR; A1R; AA1R; ARA1; Ri"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5793..5798
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Adora1"
                     /gene_synonym="A1-AR; A1AR; A1R; AA1R; ARA1; Ri"
                     /note="hexamer: AATAAA"
     regulatory      5803..5808
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Adora1"
                     /gene_synonym="A1-AR; A1AR; A1R; AA1R; ARA1; Ri"
                     /note="hexamer: ATTAAA"
     polyA_site      5812
                     /gene="Adora1"
                     /gene_synonym="A1-AR; A1AR; A1R; AA1R; ARA1; Ri"
                     /note="major polyA site"
     polyA_site      5819
                     /gene="Adora1"
                     /gene_synonym="A1-AR; A1AR; A1R; AA1R; ARA1; Ri"
ORIGIN      
        1 ctcccggtgt gcggagcccg attgtcactc agctcccgcg ccgggggcga cgaaggcgcc
       61 ggcgccagag tggagtctcg gctggctacc accccttgga cataactggc tagccctggg
      121 ttcccctggc atcctctgag ggagcgactg cccaaggttt tgaggtctgg ggatacttgg
      181 ctagacagtt caggtggcca ggcgctctgc cagctttggt gtgaccttgg gtaagtctgt
      241 ggctcagttc gtgcatcatg agtgtgcagg aaaggggtgg atgaaatgac tggggtgtcc
      301 cccttggggc agtcgatggg taaggattct gggcagagct ggagctggac caggcttctg
      361 ttaagaatcg ggcaccagcg gcgtcccagg tgggtgggca cagggtaggc gtgcgcgcac
      421 gcggggaatg ggagccggtg gacccgccag gtcctgggct tagtccctca gaatcacgcc
      481 tgccatccag tcccaggtac gaaacagggg cactacctct ttaaaggcgt ccggggctga
      541 gcctctgcca caccatgtga ttgcttgaaa ggccgggctg ggagcgcagc ggcgggagcc
      601 tggagaacga tgagccgatg cgcgcgcggt gcccagaacc caaccctgcc cggcgcggct
      661 ccggaaagcc gcccggggaa cttagggcaa cgcctttggg accccctacc ggttggcagg
      721 caggatggtg agctccttgc ctccgggtcg gcggacacct gtgggcactg tggattcaca
      781 ggggatcctt gtggagtaag tgtacaggga gccacctcac acctgataca aaaaccagtg
      841 gtggagtgag tgctgctatt ttaagttgct gaatggaacc tctaaggaca aggggagggg
      901 acagaggtta agcagatagt gagcctgagt gtctggccag cttgggcagg cactgcatca
      961 ctttactggg gcacatcttc gcagggctgc ctatgtcccc ctccttccca ggctggcatc
     1021 cccaccgcag gcttcctgac cacaggtgct ggcctcgcgc cccagggcgc ctgtctgctg
     1081 atgtgcccag ctcctgccca ccatgccgcc gtacatctcg gccttccagg ctgcctacat
     1141 tggcatcgag gtgctcattg ccttggtctc tgtgcccgga aatgtactgg tgatttgggc
     1201 tgtgaaggtg aaccaggcac ttcgcgatgc taccttctgc ttcatcgtat ccctggcggt
     1261 agctgatgtg gctgttggcg ccctggtcat cccactggcc atccttatca acattgggcc
     1321 acagacctac ttccacacct gcctcatggt ggcctgtcct gtcctcatcc tcacccagag
     1381 ctccatcctg gctctgcttg ctattgctgt ggatcggtac ctccgagtca agatccctct
     1441 ccggtacaag acagtggtga ctcagcggcg ggcagcagtg gccatagccg gctgctggat
     1501 tctctccctt gtggtaggcc tgacacccat gtttggctgg aacaacctga gtgaggtgga
     1561 acaagcctgg atagccaacg gcagtgttgg ggagcccgtg atcaagtgtg agttcgagaa
     1621 ggttatcagc atggagtaca tggtctactt caacttcttc gtctgggtgc tgccgccatt
     1681 gctcctcatg gttcttatct acctggaggt cttctacctg atccgcaagc agctcaacaa
     1741 aaaggtgtca gcctcctctg gtgaccccca gaagtactac gggaaggagc tcaagatcgc
     1801 caagtcgctg gccctcatcc tcttcctctt tgccctcagc tggctaccgc tacacatctt
     1861 gaactgcatc accctcttct gccccacctg ccagaaaccc agcatcctca tctacattgc
     1921 catcttcctc acacacggca actcggccat gaaccccatc gtctatgcct tccgaatcca
     1981 caagttccgg gtcacctttc tgaagatttg gaacgaccac ttccgatgcc agcccaagcc
     2041 ccccattgag gaagatatcc cagaggagaa agctgatgac taggctctgc cttgctccat
     2101 ctagcccaca cccagtggct ctctgttcaa cccccatgtc ctccctgttc ccccctgtcc
     2161 cactgttcct cctcagcctt cccagctggg ctgtgggctg tggacaccag ggaggctctg
     2221 aagagatgcc atggaacagg gccccttctg taaggagctg gctgccctgt accttgggga
     2281 cctggaggag gtttggagtt ggggcaggcc atagcaggga attgggtgcc ctcaggtgag
     2341 aggagagtct tgtgtgcatc attcctggct gttctctgag cagttcagag agctggggtt
     2401 agaatattct ggctccttca gtcagacagg ggagcctact attaggtgtt ggtggcccaa
     2461 gggaccaact tcaaagtgag gaagacagtc ccttagaggt ggatgaatag agagaaatgg
     2521 atatgccgcc gcttcgctgt ctctcctgtt ctctaggaga agatagtaaa acacagggaa
     2581 ggagacctcg gcccctcctc caaggattgt gttcttggcc acgcgggtgc tctcctgacc
     2641 ccgagaagcc cagggttata cttgggagac acagaaagaa tgggtccagg gattgtctct
     2701 cacatttgct ctattcacag cctgggactc ccagtgggcc ataaagtcct tgggaataaa
     2761 aatagtgcag aaggtgctgg cctcacatag ccagcaggga gcagcactga gcccttcttg
     2821 ccctgaactt cctgggagtc tccaatgcaa ttttctgtct gggccaccaa gcttcaccaa
     2881 ctctcagagc aggcctggac tctcctgagg gacaccatct ctgcttccaa gccccaggag
     2941 aagagcttcc ccaggaagta caacccttgg gagcatgctg cctgaggggg ggaggaggga
     3001 gaggaaagca tctaagaaga tcccaggtgc tcctgggcac ctctggtgtg cccagaagtg
     3061 tgaaagacca gaccagacca gaccagacca gaccagacca gaccaaagga gactgctgtc
     3121 ttcagtgtga gaaagtgcct tgctggcatt taaatatcca aaaggctcct ggaggtggct
     3181 ttagctcagg agctgtgttg caaatagagt gtaggctttt gtggaggtct caggtgggac
     3241 caatccatgt tctgctgagc ctggatgtct tccttgtatg gacagcaggc aggggagggt
     3301 gtcaggttct cccctccttg ggtgtgaata ttgactccaa gtcttgattc caggctcttc
     3361 cttcagttgg aggtaaggtg tccctggctc tcaaggagga ccctatgtct aataaaagat
     3421 tgtgaagccc tgtggggagt ggggtgctgg caccccagca ctgccagagg gcaaccaaac
     3481 cacctcagcg gagaagatgt aatggtgggg gtggagggga agctgggaga gggctgtgga
     3541 ggcagatgga aagtcccaga gatggtgtgt ctgttgctat agttacacat ttagggtagc
     3601 acattgtgaa tcagaaagca agatccagaa cactcacatg tctaaaaaag aaagaaagaa
     3661 aaaaaaagaa aaaaaaatgc cccgtgtcat gtgagaccct tcctgctggc acttttctaa
     3721 tcttgatagt gtctcccctt catagtaaca tagtattgtt ccatccgtca tgacactcat
     3781 tgccacagaa accccatgct ataacaccac acacctttat ttgaatactt ctcatacagt
     3841 gtacaactct gaagagggtg ccagtctctg ccccttctgg gccctcttga acccagaagg
     3901 tatggcctgg ggcctgccag gcctgatcct gggtctggac ccagcgtctg atggaggggg
     3961 cctgcaggta ctagagaccc cgccccattc gctgttccta tggtcttggg gattcctggg
     4021 gtcctgatcc tcaggtctgc aggacttggt ggatagtgtc cttctgcgtc ctggtatgtg
     4081 accaagctgc tagaagagat agtggggagt cgggtagaga accacctcca cccttctcag
     4141 tccttcctca ggctcaattt ccaaagctgg gctctgccca gtgcagctct ggagacttga
     4201 tagttcaccc agagcctcag aaccaccaaa cgaggaaggg agagcaagga ctgggtccct
     4261 actttcctaa tgagggaaaa gaatgctgtt tacagtcact cagagccaag cccagatgct
     4321 gacctgtgac caccacccag agtacccatc ccttctggac ctccccagca tgacccagtg
     4381 ctacactgtc ttcaccaaac taaacatgca tgctctctgt cttttgctac aaaaatggta
     4441 cccagatctg cctactgctt tcctgccctc ccctccctgt ctccagtgac attttatgcc
     4501 tgggaggtgg attcttctct cgcaatcttc tctttgacac cacactctca gtagaggtaa
     4561 ctgaaggagt aggtccagcc actcacctgt ctccttgtcc cagtctctgc ctctgttgct
     4621 ctttagtgtg tggcagaagc tgtaagagat actacacgtt agagctttgg gggaagacag
     4681 gagggaacgg agaggtttga ggtcaagaat ttccagcacc ccagtcccca aatagaaaga
     4741 acatcttccc acttagaaag tcacacaaac agttcttgac ccctccctac ccttttcctg
     4801 ctccagtttc actctgaact acatctagtc cagctcccaa gagggactca gtctgtccac
     4861 tacagcaggg tgctgagggc acccttccga ctcccacctt taacctccag ccgacaatcc
     4921 acagaggcct cccctagcac gttgatggcc ttgcaagtgt agaccccaga atcaaagggg
     4981 ctgggcttcc ggatctccag ggtgcagacc ccttgctcgg agacggcacg gtacttgggg
     5041 tcaccctgga tgtccatctt gtttttcatc cagatgatct tgggctggaa gcaaagtcag
     5101 aggagggtaa tgaagttaga acagctctca agagaaactt tcattcctct gtccacccac
     5161 tccaccagcc cagggcccct gaccttaggt gatgctcgga cgctgcagaa aagctgcgtg
     5221 ctatagccag gagtggaggt atggtcagcc aggggctggg tgaacgaggg cgcttctgag
     5281 aagtctcgct caataaactc tctaggtttg gcagtaatat ctaggttcag gggagaattt
     5341 gaggttatag ggatgcagga ggcttcggaa gactggcctc aacccaaaac tttgagacat
     5401 tcaacctggt caacacatga agacatcaga gaaaagcctc gctgagcagg gctgaggctg
     5461 atcaggaggt gaacagagag gagtgggcag gaaaacctga gccacggagg attgcggcaa
     5521 gaggcggaca tcacattcca tttctgtctg cctctgagac ctgagtggct ttccttttta
     5581 aacacaaaat gctcacggct gtgacactct ggctgatgtt aaagagtatg gcttctaggg
     5641 tgagtgaggt ggctcagcgg gtgagggtgc ttgtcaccaa gcttaccacc ctgagttcag
     5701 tccctgggac ccacggggta gaaggagaga cctgacatgt tgtccttaga cttacacatg
     5761 cacgtgtgca tgtgaacaca cactcacaca ccaataaatg taattaaaat gagtagtaaa
     5821 aaaaaaaaaa aaaaaaa
//
