LOCUS       NM_175236               3254 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus alcohol dehydrogenase, iron containing, 1 (Adhfe1),
            transcript variant 1, mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_175236
VERSION     NM_175236.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3254)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3254)
  AUTHORS   Nabeshima,R., Nishimura,O., Maeda,T., Shimizu,N., Ide,T.,
            Yashiro,K., Sakai,Y., Meno,C., Kadota,M., Shiratori,H., Kuraku,S.
            and Hamada,H.
  TITLE     Loss of Fam60a, a Sin3a subunit, results in embryonic lethality and
            is associated with aberrant methylation at a subset of gene
            promoters
  JOURNAL   Elife 7, e36435 (2018)
   PUBMED   30070635
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3254)
  AUTHORS   Thoren,L.A., Norgaard,G.A., Weischenfeldt,J., Waage,J.,
            Jakobsen,J.S., Damgaard,I., Bergstrom,F.C., Blom,A.M., Borup,R.,
            Bisgaard,H.C. and Porse,B.T.
  TITLE     UPF2 is a critical regulator of liver development, function and
            regeneration
  JOURNAL   PLoS One 5 (7), e11650 (2010)
   PUBMED   20657840
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3254)
  AUTHORS   Kim,J.Y., Tillison,K.S., Zhou,S., Lee,J.H. and Smas,C.M.
  TITLE     Differentiation-dependent expression of Adhfe1 in adipogenesis
  JOURNAL   Arch Biochem Biophys 464 (1), 100-111 (2007)
   PUBMED   17559793
  REMARK    GeneRIF: Adhfe1 is a novel alcohol dehydrogenase with mitochondrial
            localization
REFERENCE   5  (bases 1 to 3254)
  AUTHORS   Keng,V.W., Yae,K., Hayakawa,T., Mizuno,S., Uno,Y., Yusa,K.,
            Kokubu,C., Kinoshita,T., Akagi,K., Jenkins,N.A., Copeland,N.G.,
            Horie,K. and Takeda,J.
  TITLE     Region-specific saturation germline mutagenesis in mice using the
            Sleeping Beauty transposon system
  JOURNAL   Nat Methods 2 (10), 763-769 (2005)
   PUBMED   16179923
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC103620.12, AK038853.1 and AW494922.1.
            
            On Oct 26, 2017 this sequence version replaced NM_175236.4.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longer isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK038853.1, BC026584.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-132               AC103620.12        39370-39501
            133-2214            AK038853.1         2-2083
            2215-3237           AK038853.1         2085-3107
            3238-3254           AW494922.1         1-17                c
FEATURES             Location/Qualifiers
     source          1..3254
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 2.08 cM"
     gene            1..3254
                     /gene="Adhfe1"
                     /gene_synonym="6330565B14Rik; Adh8; HOT"
                     /note="alcohol dehydrogenase, iron containing, 1"
                     /db_xref="GeneID:76187"
                     /db_xref="MGI:MGI:1923437"
     exon            1..204
                     /gene="Adhfe1"
                     /gene_synonym="6330565B14Rik; Adh8; HOT"
                     /inference="alignment:Splign:2.1.0"
     CDS             146..1543
                     /gene="Adhfe1"
                     /gene_synonym="6330565B14Rik; Adh8; HOT"
                     /EC_number="1.1.99.24"
                     /note="isoform 1 is encoded by transcript variant 1;
                     alcohol dehydrogenase 8; Fe-containing alcohol
                     dehydrogenase 1; hydroxyacid-oxoacid transhydrogenase,
                     mitochondrial; alcohol dehydrogenase iron-containing
                     protein 1"
                     /codon_start=1
                     /product="hydroxyacid-oxoacid transhydrogenase,
                     mitochondrial isoform 1"
                     /protein_id="NP_780445.1"
                     /db_xref="CCDS:CCDS14812.1"
                     /db_xref="GeneID:76187"
                     /db_xref="MGI:MGI:1923437"
                     /translation="MAAAARARVTHLLRHLQSTACQCPTHSHTYSQAPGPSGKTADYA
                     FEMAVSNIRYGAGVTKEVGMDLQNMGAKNVCLMTDKNLSQLPPVQIVMDSLSKNGISF
                     QVYDDVRVEPTDGSFMDAIEFAKKGAFDAYVAVGGGSTMDTCKAANLYASSPHSEFLD
                     YVNAPIGKGKPVTVPLKPLIAVPTTSGTGSETTGVAIFDYEHLKVKTGIASRAIKPTL
                     GLVDPLHTLHMPCQVVANSGFDVLCHALESYTAIPYSMRSPCPSNPIQRPAYQGSNPI
                     SDIWAVHALQIVAKYLKRAVRNPDDLEARSKMHLASAFAGIGFGNAGVHLCHGMSYPI
                     SGLVKTYKAKEYNVDHPLVPHGLSVVLTSPAVFTFTAQMFPERHLETAGILGANIRTA
                     RIQDAGLVLADALRKFLFDLNVDDGLAALGYSKDDIPSLVKGTLPQERVTKLAPRAQS
                     EEDLSALFEASMKLY"
     exon            205..242
                     /gene="Adhfe1"
                     /gene_synonym="6330565B14Rik; Adh8; HOT"
                     /inference="alignment:Splign:2.1.0"
     exon            243..283
                     /gene="Adhfe1"
                     /gene_synonym="6330565B14Rik; Adh8; HOT"
                     /inference="alignment:Splign:2.1.0"
     exon            284..337
                     /gene="Adhfe1"
                     /gene_synonym="6330565B14Rik; Adh8; HOT"
                     /inference="alignment:Splign:2.1.0"
     exon            338..492
                     /gene="Adhfe1"
                     /gene_synonym="6330565B14Rik; Adh8; HOT"
                     /inference="alignment:Splign:2.1.0"
     exon            493..689
                     /gene="Adhfe1"
                     /gene_synonym="6330565B14Rik; Adh8; HOT"
                     /inference="alignment:Splign:2.1.0"
     exon            690..767
                     /gene="Adhfe1"
                     /gene_synonym="6330565B14Rik; Adh8; HOT"
                     /inference="alignment:Splign:2.1.0"
     exon            768..873
                     /gene="Adhfe1"
                     /gene_synonym="6330565B14Rik; Adh8; HOT"
                     /inference="alignment:Splign:2.1.0"
     exon            874..1026
                     /gene="Adhfe1"
                     /gene_synonym="6330565B14Rik; Adh8; HOT"
                     /inference="alignment:Splign:2.1.0"
     exon            1027..1125
                     /gene="Adhfe1"
                     /gene_synonym="6330565B14Rik; Adh8; HOT"
                     /inference="alignment:Splign:2.1.0"
     exon            1126..1204
                     /gene="Adhfe1"
                     /gene_synonym="6330565B14Rik; Adh8; HOT"
                     /inference="alignment:Splign:2.1.0"
     exon            1205..1301
                     /gene="Adhfe1"
                     /gene_synonym="6330565B14Rik; Adh8; HOT"
                     /inference="alignment:Splign:2.1.0"
     exon            1302..1459
                     /gene="Adhfe1"
                     /gene_synonym="6330565B14Rik; Adh8; HOT"
                     /inference="alignment:Splign:2.1.0"
     exon            1460..3239
                     /gene="Adhfe1"
                     /gene_synonym="6330565B14Rik; Adh8; HOT"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1980..1985
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Adhfe1"
                     /gene_synonym="6330565B14Rik; Adh8; HOT"
                     /note="hexamer: AATAAA"
     regulatory      1991..1996
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Adhfe1"
                     /gene_synonym="6330565B14Rik; Adh8; HOT"
                     /note="hexamer: AGTAAA"
     polyA_site      2002
                     /gene="Adhfe1"
                     /gene_synonym="6330565B14Rik; Adh8; HOT"
     polyA_site      2007
                     /gene="Adhfe1"
                     /gene_synonym="6330565B14Rik; Adh8; HOT"
                     /note="major polyA site"
     regulatory      2168..2173
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Adhfe1"
                     /gene_synonym="6330565B14Rik; Adh8; HOT"
                     /note="hexamer: AATAAA"
     regulatory      2177..2182
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Adhfe1"
                     /gene_synonym="6330565B14Rik; Adh8; HOT"
                     /note="hexamer: AAGAAA"
     polyA_site      2186
                     /gene="Adhfe1"
                     /gene_synonym="6330565B14Rik; Adh8; HOT"
     polyA_site      2199
                     /gene="Adhfe1"
                     /gene_synonym="6330565B14Rik; Adh8; HOT"
     regulatory      3219..3224
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Adhfe1"
                     /gene_synonym="6330565B14Rik; Adh8; HOT"
                     /note="hexamer: AATGAA"
     polyA_site      3239
                     /gene="Adhfe1"
                     /gene_synonym="6330565B14Rik; Adh8; HOT"
ORIGIN      
        1 agacatttca gagtttcctg agaaatgagc taagactggg tcaggtgacc ttgcattgga
       61 ggcagaagtt ttcctcctgg agtccagcag cgaccggatt ggctgttagt gggggtggag
      121 taggcggcga acaaggagaa ctgccatggc tgcagctgca cgcgctcgag tcacacactt
      181 gctgaggcac ctacaaagca cagcatgcca gtgcccaact cattctcata cttactccca
      241 agcccctgga ccttcgggaa aaacagcaga ttacgccttt gagatggctg tctcaaatat
      301 tagatatgga gcaggggtta cgaaggaagt aggcatggat ctacagaata tgggagctaa
      361 gaatgtttgc ttgatgacag acaagaacct ctcccagctc cctcctgtac aaatagttat
      421 ggattcccta tcgaagaatg gcatcagttt tcaggtttat gatgatgtaa gggtggaacc
      481 tacagatgga agcttcatgg atgcaattga atttgccaaa aagggagcgt ttgatgccta
      541 tgttgctgtg ggtgggggct ccaccatgga cacctgtaaa gccgccaatc tgtatgcatc
      601 cagccctcac tctgagttcc ttgattatgt caatgccccc attgggaagg ggaagccagt
      661 aactgtgcct cttaaacctc tgattgcagt tccaactacc tcagggactg gcagtgagac
      721 taccggggtt gccatttttg actatgagca tttgaaagta aaaactggca ttgcttcacg
      781 agccatcaaa cccaccctgg gactggttga tcccctgcac actctccaca tgccttgcca
      841 ggtggttgcc aacagtggct tcgatgtcct ttgccatgct ctggagtcat acaccgccat
      901 tccctacagc atgcggagcc cctgcccttc caaccccatc caacgaccag cataccaggg
      961 tagcaaccca atcagcgaca tctgggcagt ccatgcactg cagattgttg ccaaatatct
     1021 gaagagggct gtcagaaacc cagatgatct ggaagcaagg tctaaaatgc acttggcaag
     1081 tgcttttgct ggcattggct tcggaaatgc tggtgttcat ctgtgccatg gcatgtctta
     1141 cccaatttca ggtttagtga agacatataa agccaaggaa tataatgtgg atcaccctct
     1201 tgtgccccat ggcctttctg tggtgctcac ctctcctgca gtgttcacct tcacagccca
     1261 gatgtttcca gagcggcacc tggagacggc aggaatatta ggagccaaca tccgcactgc
     1321 caggatccaa gatgctgggc ttgtgttggc agatgccctc cgaaaattcc tatttgacct
     1381 aaatgttgat gacggtctgg ctgctcttgg ttattccaag gatgacattc cttcattggt
     1441 gaaaggaaca ctgccccagg aaagggtcac gaagctcgca cctcgtgccc agtcagaaga
     1501 ggatttgtct gctttgtttg aagcatccat gaaactgtac taattaacat attagtgaaa
     1561 agaatcccca ttggcctttg cgacagaagt tacaaatagc agaagttggc cagagctgat
     1621 gttgccgtct tatctccaca cttaactcac ctattgctgg tttgaatgtg atgtgatttt
     1681 atcctttgga agattccttg gtgctcatta catggaaccc atgggccata ccccaggggt
     1741 cagtattcct gtaccaaacc ccatcaaata cataaaaatg cctatctcca aacactgcat
     1801 gtctagacat ttaccctgaa gaaatactag cattagtgtg caaaaaaaaa acaatgcata
     1861 ctaggttgca cacaaggatg agtctgacat atttttagat agtaaaaatt aaaacaatct
     1921 aaatattcaa tactgaatga atcctaatat agtaaatgat agaatactgt ggtattatta
     1981 ataaaaatga agtaaaactc ttgaaaatat gtctgtttca taaaagctgg tgtcaggaaa
     2041 caagtggcag aaaggctata aagaagagtc tgacttaact tttcaactgt atatgagtgt
     2101 gtctatatag agaaaaaggt gcacattaaa atattaaaag atatcagaga agagaattca
     2161 ggagtataat aaattaaaga aaaaaatgtg tactgagtat tttatgcttc caggtttttt
     2221 taaggtgctg aaattagaac ccaggatctt gcacacatta ggcaagtaat ctaccactga
     2281 gccacattct cagctcttgg gttttaaggt agagtcactc tgcatgtctt cggtttaagg
     2341 ctggttttga atttggggtt cttctgcctc tgtcttccca gcgctgagat tatgcaccat
     2401 gcctcacctg gtctcccatt ttgtatttta tacatactta ttgtctgggc ttgttaaaga
     2461 aaacaatttc ctaatcttaa tgggaaaggc agggttggga tgatgactta gtaggcaaag
     2521 tgcttgccat ccatatgtga agatttgagt tcggatcctc ccctcacatc catgtaaatg
     2581 ccagccacag ctttgtacat ctgtaaactt ggcactgtgg tggtaggtag agacaagcag
     2641 aaccccaggg ctcactggtc agccagttga gattcatcga gagattctgt ctcagaatat
     2701 aaggtagaaa gtaacaggaa gacatctaat gtctacctct gatctctaca tgcgcacaca
     2761 aacacacatg aacacatcat actacacaca cacaatcacc tgtgagccat atcaccattt
     2821 accctacgaa cagcacttca caagactcaa ggcaagcaat ggcagctagt gcaggattgc
     2881 tgtgcagggc aggaggctcc agcaggatgt ggctacacat ctacttgatg gacagcacac
     2941 tccaccttgc tcggtggaaa tttgtgaaaa ctttttcaat ttggaattgg ttgactttca
     3001 gatgtagagc ctgtgagtaa tgtctttcct gtactgctcc acggtgttat ttagagcccc
     3061 tggggtaccg gatttctgtt cctgtcacct gaacctttat ttaaccaacc cctcgttagc
     3121 ttgaaaatgt tataagtata ttttatatat tatctactct tgaatgtgag attcttttta
     3181 attcaagaaa tttaatgaaa aacaatttga gatataaaaa tgaattcttt atccttcaaa
     3241 aaaaaaaaaa aaaa
//
