LOCUS       NP_848827               1009 aa            linear   ROD 08-APR-2025
DEFINITION  adhesion G-protein coupled receptor G2 isoform 1 precursor [Mus
            musculus].
ACCESSION   NP_848827
VERSION     NP_848827.1
DBSOURCE    REFSEQ: accession NM_178712.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (residues 1 to 1009)
  AUTHORS   Kiyozumi,D. and Ikawa,M.
  TITLE     Adhesion G protein-coupled receptor G2 is dispensable for lumicrine
            signaling regulating epididymal initial segment differentiation and
            gene expressiondagger
  JOURNAL   Biol Reprod 109 (4), 474-481 (2023)
   PUBMED   37531264
REFERENCE   2  (residues 1 to 1009)
  AUTHORS   Lin,H., Xiao,P., Bu,R.Q., Guo,S., Yang,Z., Yuan,D., Zhu,Z.L.,
            Zhang,C.X., He,Q.T., Zhang,C., Ping,Y.Q., Zhao,R.J., Ma,C.S.,
            Liu,C.H., Zhang,X.N., Jiang,D., Huang,S., Xi,Y.T., Zhang,D.L.,
            Xue,C.Y., Yang,B.S., Li,J.Y., Lin,H.C., Zeng,X.H., Zhao,H.,
            Xu,W.M., Yi,F., Liu,Z., Sun,J.P. and Yu,X.
  TITLE     Structures of the ADGRG2-Gs complex in apo and ligand-bound forms
  JOURNAL   Nat Chem Biol 18 (11), 1196-1203 (2022)
   PUBMED   35982227
REFERENCE   3  (residues 1 to 1009)
  AUTHORS   Xiao,P., Guo,S., Wen,X., He,Q.T., Lin,H., Huang,S.M., Gou,L.,
            Zhang,C., Yang,Z., Zhong,Y.N., Yang,C.C., Li,Y., Gong,Z., Tao,X.N.,
            Yang,Z.S., Lu,Y., Li,S.L., He,J.Y., Wang,C., Zhang,L., Kong,L.,
            Sun,J.P. and Yu,X.
  TITLE     Tethered peptide activation mechanism of the adhesion GPCRs ADGRG2
            and ADGRG4
  JOURNAL   Nature 604 (7907), 771-778 (2022)
   PUBMED   35418677
REFERENCE   4  (residues 1 to 1009)
  AUTHORS   Grunddal,K.V., Tonack,S., Egerod,K.L., Thompson,J.J., Petersen,N.,
            Engelstoft,M.S., Vagne,C., Keime,C., Gradwohl,G., Offermanns,S. and
            Schwartz,T.W.
  TITLE     Adhesion receptor ADGRG2/GPR64 is in the GI-tract selectively
            expressed in mature intestinal tuft cells
  JOURNAL   Mol Metab 51, 101231 (2021)
   PUBMED   33831593
  REMARK    GeneRIF: Adhesion receptor ADGRG2/GPR64 is in the GI-tract
            selectively expressed in mature intestinal tuft cells.
REFERENCE   5  (residues 1 to 1009)
  AUTHORS   Sun,Y., Zhang,D., Ma,M.L., Lin,H., Song,Y., Wang,J., Ma,C., Yu,K.,
            An,W., Guo,S., He,D., Yang,Z., Xiao,P., Hou,G., Yu,X. and Sun,J.P.
  TITLE     Optimization of a peptide ligand for the adhesion GPCR ADGRG2
            provides a potent tool to explore receptor biology
  JOURNAL   J Biol Chem 296, 100174 (2021)
   PUBMED   33303626
  REMARK    GeneRIF: Optimization of a peptide ligand for the adhesion GPCR
            ADGRG2 provides a potent tool to explore receptor biology.
REFERENCE   6  (residues 1 to 1009)
  AUTHORS   Davies,B., Behnen,M., Cappallo-Obermann,H., Spiess,A.N.,
            Theuring,F. and Kirchhoff,C.
  TITLE     Novel epididymis-specific mRNAs downregulated by HE6/Gpr64 receptor
            gene disruption
  JOURNAL   Mol Reprod Dev 74 (5), 539-553 (2007)
   PUBMED   17034053
  REMARK    GeneRIF: The knockout of the HE6/Gpr64 receptor was mainly
            associated with the downregulation of genes specific to the initial
            segment.
REFERENCE   7  (residues 1 to 1009)
  AUTHORS   Eng,S.R., Dykes,I.M., Lanier,J., Fedtsova,N. and Turner,E.E.
  TITLE     POU-domain factor Brn3a regulates both distinct and common programs
            of gene expression in the spinal and trigeminal sensory ganglia
  JOURNAL   Neural Dev 2, 3 (2007)
   PUBMED   17239249
  REMARK    Publication Status: Online-Only
REFERENCE   8  (residues 1 to 1009)
  AUTHORS   Davies,B., Baumann,C., Kirchhoff,C., Ivell,R., Nubbemeyer,R.,
            Habenicht,U.F., Theuring,F. and Gottwald,U.
  TITLE     Targeted deletion of the epididymal receptor HE6 results in fluid
            dysregulation and male infertility
  JOURNAL   Mol Cell Biol 24 (19), 8642-8648 (2004)
   PUBMED   15367682
REFERENCE   9  (residues 1 to 1009)
  AUTHORS   Bjarnadottir,T.K., Fredriksson,R., Hoglund,P.J., Gloriam,D.E.,
            Lagerstrom,M.C. and Schioth,H.B.
  TITLE     The human and mouse repertoire of the adhesion family of
            G-protein-coupled receptors
  JOURNAL   Genomics 84 (1), 23-33 (2004)
   PUBMED   15203201
REFERENCE   10 (residues 1 to 1009)
  AUTHORS   Obermann,H., Samalecos,A., Osterhoff,C., Schroder,B., Heller,R. and
            Kirchhoff,C.
  TITLE     HE6, a two-subunit heptahelical receptor associated with apical
            membranes of efferent and epididymal duct epithelia
  JOURNAL   Mol Reprod Dev 64 (1), 13-26 (2003)
   PUBMED   12420295
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY769670.1, BC116644.1 and AL731801.11.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR6116558.98483.1,
                                           SRR17253012.8829253.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849381, SAMN00849383
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Mus musculus"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="X"
                     /map="X 73.95 cM"
     Protein         1..1009
                     /product="adhesion G-protein coupled receptor G2 isoform 1
                     precursor"
                     /note="LNB-TM7 heptahelical receptor Me6; G-protein
                     coupled receptor 64; epididymis-specific protein 6; G
                     protein coupled receptor He6"
                     /calculated_mol_wt=106035
     sig_peptide     1..37
                     /note="/evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q8CJ12.1)"
                     /calculated_mol_wt=4183
     mat_peptide     38..1009
                     /product="Adhesion G-protein coupled receptor G2.
                     /id=PRO_0000012887"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8CJ12.1)"
                     /calculated_mol_wt=106035
     Site            43
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q8CJ12.1)"
     Site            77
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q8CJ12.1)"
     Site            91
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q8CJ12.1)"
     Site            103
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q8CJ12.1)"
     Site            109
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q8CJ12.1)"
     Site            127
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q8CJ12.1)"
     Site            136
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q8CJ12.1)"
     Site            154
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q8CJ12.1)"
     Site            178
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q8CJ12.1)"
     Site            186
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q8CJ12.1)"
     Region          <242..382
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          279..305
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8CJ12.1)"
     Region          <289..483
                     /region_name="PRK12323"
                     /note="DNA polymerase III subunit gamma/tau; cl46901"
                     /db_xref="CDD:481241"
     Region          325..346
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8CJ12.1)"
     Site            362
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q8CJ12.1)"
     Site            427
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q8CJ12.1)"
     Site            448
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q8CJ12.1)"
     Site            453
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q8CJ12.1)"
     Site            520
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q8CJ12.1)"
     Site            534
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q8CJ12.1)"
     Site            539
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q8CJ12.1)"
     Site            543
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q8CJ12.1)"
     Region          560..604
                     /region_name="GPS"
                     /note="GPCR proteolysis site, GPS, motif; pfam01825"
                     /db_xref="CDD:460350"
     Region          562..611
                     /region_name="GPS.
                     /evidence=ECO:0000255|PROSITE-ProRule:PRU00098"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8CJ12.1)"
     Site            589
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q8CJ12.1)"
     Site            598..599
                     /site_type="cleavage"
                     /note="Cleavage, by autolysis.
                     /evidence=ECO:0000255|PROSITE-ProRule:PRU00098; propagated
                     from UniProtKB/Swiss-Prot (Q8CJ12.1)"
     Region          617..886
                     /region_name="7tmB2_GPR64"
                     /note="orphan adhesion receptor GPR64 and related
                     proteins, member of subfamily B2 of the class B
                     secretin-like receptors of seven-transmembrane G
                     protein-coupled receptors; cd15444"
                     /db_xref="CDD:320560"
     Region          619..644
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:320560"
     Site            620..640
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8CJ12.1)"
     Site            order(624,628,671,674..675,678,687,694,778..779,781,783,
                     838,841,853,857)
                     /site_type="other"
                     /note="putative polypeptide ligand binding pocket
                     [polypeptide binding]"
                     /db_xref="CDD:320560"
     Region          654..676
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:320560"
     Site            660..680
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8CJ12.1)"
     Site            684..704
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8CJ12.1)"
     Region          687..714
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:320560"
     Region          729..745
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:320560"
     Site            730..750
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8CJ12.1)"
     Region          775..798
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:320560"
     Site            782..802
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8CJ12.1)"
     Region          821..846
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:320560"
     Site            827..847
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8CJ12.1)"
     Site            849
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q8CJ12.1)"
     Region          850..875
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:320560"
     Site            850..870
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8CJ12.1)"
     Site            1002
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:21183079; propagated from
                     UniProtKB/Swiss-Prot (Q8CJ12.1)"
     CDS             1..1009
                     /gene="Adgrg2"
                     /gene_synonym="B830041D06Rik; Gpr64; Me6"
                     /coded_by="NM_178712.4:107..3136"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1"
                     /db_xref="CCDS:CCDS41196.1"
                     /db_xref="GeneID:237175"
                     /db_xref="MGI:MGI:2446854"
ORIGIN      
        1 mlfsggqysp vgrpeevlli ykiflviicf hvilvtslke ngnssllsps aesslvslip
       61 ysngtpdaas evlstlnkte kskitivktf nasgvksqrn icnlsslcnd svffrgeivf
      121 qhdedhnvtq nqdtangtfa gvlslselkr selnktlqtl setyfivcat aeaqstvnct
      181 ftvklnetmn vcammvtfqt vqirpmeqcc csprtpcpss peeleklqce lqdpivclad
      241 qphgpplsss skpvvpqati ishvasdfsl aepldhalmt pstpsltqes nlpspqptip
      301 lasspatdlp vqsvvvsslp qtdlshtlsp vqssipsptt papsvptelv tistppgetv
      361 vntstvsdle aqvsqmekal slgslepnla gemvnrvskl lhsppallap laqrllkvvd
      421 aiglqlnfss ttisltspsl alavirvnas nfntttfaaq dptnlqvsle tpppensiga
      481 itlpsslmnn lpandvelas riqfnffetp alfqdpslen ltlisyviss svtnmtiknl
      541 trnvtvalkh inpspddltv kcvfwdlgrn ggkggwssdg csvkdkrmne tictcshlts
      601 fgilldlsrt slppsqmmal tfityigcgl ssiflsvtlv tyiafekirr dypskiliql
      661 caallllnli flldswialy ntrgfciava vflhyfllvs ftwmgleafh mylalvkvfn
      721 tyirkyilkf civgwgipav vvsivltisp dnygigsygk fpngtpddfc winsnvvfyi
      781 tvvgyfcvif llnvsmfivv lvqlcrikkk kqlgaqrkts iqdlrsiagl tfllgitwgf
      841 affawgpvnv tfmylfaifn tlqgffifif ycaakenvrk qwrrylccgk lrlaensdws
      901 ktatnglkkq tvnqgvssss nslqsscnst nsttllvnsd csvhasgngn asterngvsf
      961 svqngdvclh dltgkqhmfs dkedscngks rialrrtskr gslhfieqm
//
