LOCUS       NM_001409525            3480 bp    mRNA    linear   ROD 18-FEB-2025
DEFINITION  Mus musculus adenylate cyclase activating polypeptide 1 (Adcyap1),
            transcript variant 4, mRNA.
ACCESSION   NM_001409525 XM_030249450
VERSION     NM_001409525.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3480)
  AUTHORS   Wang,H., Lou,R., Wang,Y., Hao,L., Wang,Q., Li,R., Su,J., Liu,S.,
            Zhou,X., Gao,X., Hao,Q., Chen,Z., Xu,Y., Wu,C., Zheng,Y., Guo,Q.
            and Bai,L.
  TITLE     Parallel gut-to-brain pathways orchestrate feeding behaviors
  JOURNAL   Nat Neurosci 28 (2), 320-335 (2025)
   PUBMED   39627537
REFERENCE   2  (bases 1 to 3480)
  AUTHORS   Sparks,J., Meggyes,M., Makszin,L., Jehn,V., Lugosi,H., Reglodi,D.
            and Szereday,L.
  TITLE     Effects of PACAP Deficiency on Immune Dysfunction and Peyer's Patch
            Integrity in Adult Mice
  JOURNAL   Int J Mol Sci 25 (19), 10676 (2024)
   PUBMED   39409005
  REMARK    GeneRIF: Effects of PACAP Deficiency on Immune Dysfunction and
            Peyer's Patch Integrity in Adult Mice.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3480)
  AUTHORS   Castorina,A., Scheller,J., Keay,K.A., Marzagalli,R., Rose-John,S.
            and Campbell,I.L.
  TITLE     Increased Expression of the Neuropeptides PACAP/VIP in the Brain of
            Mice with CNS Targeted Production of IL-6 Is Mediated in Part by
            Trans-Signalling
  JOURNAL   Int J Mol Sci 25 (17), 9453 (2024)
   PUBMED   39273398
  REMARK    GeneRIF: Increased Expression of the Neuropeptides PACAP/VIP in the
            Brain of Mice with CNS Targeted Production of IL-6 Is Mediated in
            Part by Trans-Signalling.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3480)
  AUTHORS   Zhang,H.L., Sun,Y., Wu,Z.J., Yin,Y., Liu,R.Y., Zhang,J.C.,
            Zhang,Z.J., Yau,S.Y., Wu,H.X., Yuan,T.F., Zhang,L., Adzic,M. and
            Chen,G.
  TITLE     Hippocampal PACAP signaling activation triggers a rapid
            antidepressant response
  JOURNAL   Mil Med Res 11 (1), 49 (2024)
   PUBMED   39044298
  REMARK    GeneRIF: Hippocampal PACAP signaling activation triggers a rapid
            antidepressant response.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3480)
  AUTHORS   Basu,R., Elmendorf,A.J., Lorentz,B., Mahler,C.A., Lazzaro,O.,
            App,B., Zhou,S., Yamamoto,Y., Suber,M., Wann,J.C., Roh,H.C.,
            Sheets,P.L., Johnson,T.S. and Flak,J.N.
  TITLE     Ventromedial hypothalamic nucleus subset stimulates tissue
            thermogenesis via preoptic area outputs
  JOURNAL   Mol Metab 84, 101951 (2024)
   PUBMED   38729241
REFERENCE   6  (bases 1 to 3480)
  AUTHORS   Moller,K., Reimer,M., Hannibal,J., Fahrenkrug,J., Sundler,F. and
            Kanje,M.
  TITLE     Pituitary adenylate cyclase-activating peptide (PACAP) and PACAP
            type 1 receptor expression in regenerating adult mouse and rat
            superior cervical ganglia in vitro
  JOURNAL   Brain Res 775 (1-2), 156-165 (1997)
   PUBMED   9439839
REFERENCE   7  (bases 1 to 3480)
  AUTHORS   Shuto,Y., Uchida,D., Onda,H. and Arimura,A.
  TITLE     Ontogeny of pituitary adenylate cyclase activating polypeptide and
            its receptor mRNA in the mouse brain
  JOURNAL   Regul Pept 67 (2), 79-83 (1996)
   PUBMED   8958577
REFERENCE   8  (bases 1 to 3480)
  AUTHORS   Rene,F., Monnier,D., Gaiddon,C., Felix,J.M. and Loeffler,J.P.
  TITLE     Pituitary adenylate cyclase-activating polypeptide transduces
            through cAMP/PKA and PKC pathways and stimulates
            proopiomelanocortin gene transcription in mouse melanotropes
  JOURNAL   Neuroendocrinology 64 (1), 2-13 (1996)
   PUBMED   8811660
REFERENCE   9  (bases 1 to 3480)
  AUTHORS   Larsen,N.J., Womack,J.E. and Kirkpatrick,B.W.
  TITLE     Seven genes from human chromosome 18 map to chromosome 24 in the
            bovine
  JOURNAL   Cytogenet Cell Genet 73 (3), 184-186 (1996)
   PUBMED   8697804
REFERENCE   10 (bases 1 to 3480)
  AUTHORS   Okazaki,K., Itoh,Y., Ogi,K., Ohkubo,S. and Onda,H.
  TITLE     Characterization of murine PACAP mRNA
  JOURNAL   Peptides 16 (7), 1295-1299 (1995)
   PUBMED   8545254
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC154798.2.
            
            On Jul 19, 2022 this sequence version replaced XM_030249450.1.
            
            Summary: This gene encodes a member of the glucagon superfamily of
            hormones that have important roles in growth and metabolism. The
            encoded protein undergoes proteolytic processing to generate mature
            peptides that function as hypophysiotropic hormones,
            neurotransmitters, neuromodulators and vasoregulators. Mice lacking
            the encoded protein exhibit a high rate of early mortality. The
            surviving adult animals lacking the encoded protein exhibit
            decreased anxiety, hyperactive behavior and impaired
            steroidogenesis. Alternative splicing results in multiple
            transcript variants encoding different isoforms. [provided by
            RefSeq, Oct 2015].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14995048.1221734.1,
                                           SRR7345562.3730379.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN01164131
                                           [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-482               AC154798.2         85972-86453         c
            483-590             AC154798.2         85474-85581         c
            591-779             AC154798.2         83176-83364         c
            780-878             AC154798.2         82711-82809         c
            879-3480            AC154798.2         79055-81656         c
FEATURES             Location/Qualifiers
     source          1..3480
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="17"
                     /map="17 60.67 cM"
     gene            1..3480
                     /gene="Adcyap1"
                     /gene_synonym="PACAP"
                     /note="adenylate cyclase activating polypeptide 1"
                     /db_xref="GeneID:11516"
                     /db_xref="MGI:MGI:105094"
     exon            1..482
                     /gene="Adcyap1"
                     /gene_synonym="PACAP"
                     /inference="alignment:Splign:2.1.0"
     exon            483..590
                     /gene="Adcyap1"
                     /gene_synonym="PACAP"
                     /inference="alignment:Splign:2.1.0"
     CDS             484..1068
                     /gene="Adcyap1"
                     /gene_synonym="PACAP"
                     /note="isoform 3 preproprotein is encoded by transcript
                     variant 4; pituitary adenylate cyclase-activating
                     polypeptide"
                     /codon_start=1
                     /product="pituitary adenylate cyclase-activating
                     polypeptide isoform 3 preproprotein"
                     /protein_id="NP_001396454.1"
                     /db_xref="GeneID:11516"
                     /db_xref="MGI:MGI:105094"
                     /translation="MTMCSGARLALLVYGIIMHSSVSCSPAAGLSFPGIRPEDEAYDQ
                     DGNPLQDFYDWDPPGVGSPASALRDAYALYYPADRRYNPPGFRLWLGPNLAEGTGDVA
                     HEILNEAYRKVLDQLSARKYLQSVVARGAGENLGGSAVDDPAPLTKRHSDGIFTDSYS
                     RYRKQMAVKKYLAAVLGKRYKQRVKNKGRRIAYL"
     exon            591..779
                     /gene="Adcyap1"
                     /gene_synonym="PACAP"
                     /inference="alignment:Splign:2.1.0"
     exon            780..878
                     /gene="Adcyap1"
                     /gene_synonym="PACAP"
                     /inference="alignment:Splign:2.1.0"
     exon            879..3480
                     /gene="Adcyap1"
                     /gene_synonym="PACAP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2412..2417
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Adcyap1"
                     /gene_synonym="PACAP"
                     /note="hexamer: AGTAAA"
     polyA_site      2436
                     /gene="Adcyap1"
                     /gene_synonym="PACAP"
                     /note="major polyA site"
     regulatory      2486..2491
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Adcyap1"
                     /gene_synonym="PACAP"
                     /note="hexamer: GATAAA"
     polyA_site      2505
                     /gene="Adcyap1"
                     /gene_synonym="PACAP"
                     /note="major polyA site"
     regulatory      3462..3467
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Adcyap1"
                     /gene_synonym="PACAP"
                     /note="hexamer: AATAAA"
     polyA_site      3480
                     /gene="Adcyap1"
                     /gene_synonym="PACAP"
ORIGIN      
        1 gtcagcagca gaagcagcag catcttcaga cgcagcctga gacctcagag cagagaaggt
       61 gcctccgact cccgacttcc gcaagctgcc agatagtctc ccacagaggc catcttccct
      121 ctcccccccc cccccactga cttttcctct tttcttaccc attagcttct tctcccggtg
      181 gacttaagtc cactctgctc cccctccctt tggctgctcg cctactctct cttctacatc
      241 tctctcctcg cccacttcct tcggtgtcac gctccctcct agttttgtgc gtctacaaag
      301 ttttgagcgg aattttagcc tcggcaaaca agtctcccca gctccttcag ctaattcccg
      361 cggcttctct ccagacacca gctccagaca gtgactgatg cttctcgggt tgtgattcca
      421 gcgcagaaac tcgaaggagc ctttgcccgc tgtcctactt agtcaaccct ttcctagaca
      481 ccaatgacca tgtgtagcgg agcaaggctg gccctgctgg tgtatgggat aataatgcat
      541 agcagtgtct cctgttcacc tgccgccgga ctcagcttcc ctgggatcag accagaagac
      601 gaggcttacg accaggacgg aaacccgctg caagacttct atgactggga ccctcccggc
      661 gtggggagcc ccgcctccgc gctgcgtgac gcttacgccc tttactatcc agcggacagg
      721 aggtacaacc cgcccggctt ccgcctgtgg cttgggccta acctggcaga agggaccgga
      781 gatgtcgccc acgaaatcct taacgaagcc tatcgaaaag tcttggacca gctgtccgcc
      841 aggaagtacc tgcagtcggt cgtggccagg ggcgcggggg agaacctagg cggcagcgcg
      901 gtggacgacc cggcgcccct taccaaacgc cactcggacg gcatcttcac agatagctac
      961 agccgctacc gaaaacaaat ggctgtcaag aaatacttgg cggccgtgct agggaaaagg
     1021 tataaacaga gggttaaaaa caaaggacgc cgaatagcat acttgtagca gatcagctgc
     1081 cggctacctt gtgtataaaa tgaaaagtcg ttttccaaat tgactgacca gtcatcactc
     1141 gtgttctttc caaacatgta tttatgtatc aagtaaagcc attaaatgac tattttgata
     1201 ataatattgt ttttcttttt atgaagcact agagaatgca cagatatact ttgtggacca
     1261 attattgata tatattataa gtatatatat taagaatata tataagtata acagagagca
     1321 attaagatgg gtgcacaagg attgaaaatt cgcctgagct gtttatgttt ttatataaag
     1381 taaatagaga aaatagacaa ccattgtttt gaatattact cctatttttg taaactggaa
     1441 ttaaaggata gtatttttat ccacaaccgt cttgaagata ccaataatgg ccatttgtac
     1501 aaaaaacaat gatgccctgc tccaggggaa ttctgaggta atgacttggg gaattgctga
     1561 agggctttct ttccctctga gtctggggca ggctgcttga accccagcct aactaactca
     1621 agtgggcatt gtcccactgg ttgcaggggc aattccaaca atttcagttt ctttgattat
     1681 gtgtatttgt ctctcctcag actctcagcc cagaaggaaa ttctaattaa acaacagctc
     1741 tatccaaatt gtgcttctcc ccagaactca tgtcattccc tgatagaaga gttgaggaac
     1801 tgtacagaag agacaggctt ggagagagag ctctcttttc tgtacttcct gattctccag
     1861 ggaacagact attctaaggc tagggcaatt ggaacaaagt gaaagatata taagggattg
     1921 gtaaaggcag aacatgggga tttgagattt gagagttgcc tcaggtctga gaatctgggg
     1981 gcaagtctag ctcctctgta ggttccactg cctgacagat caggtgctgg tgttggaatg
     2041 aatgcaaagt acaatgtgtt tttctccagt gctgttcatg cttttcatgt tgtgaaatgg
     2101 ccaggatcct cccctttgaa cactgttctg cagaagccag ctctgttctt tgtggatttt
     2161 ctggagaccc tccttcctac ccttgccctc atgcattgtt ttagagtcat ttgccatttt
     2221 ccactcactt atcttaaatt tgtgaatgct agttattttt tgttgttgtt tgatgcaagc
     2281 agttactgtg aagtttaaga acccctgtgt agcttccaca gagaaattat gcactaaata
     2341 tgaacctttt gtttcttgtt tattgagttt gtaggtaaaa atgtattttt ctatattatg
     2401 gcttattgct tagtaaaatt tatttcataa aaccaatctt tgtcatatta gaatgtgtag
     2461 tgttccaatg ctgctcagtt tgactgataa atcatttaaa ccccatcttc atatgtatga
     2521 gtactatctt atatttgttc ttaagagtga tggaaggagg atcccacacc ccacccttgt
     2581 atcctgtctt ggctaaaaaa agcatgtctg tttcttgtca atttcttgat aaacaaaaga
     2641 accttcacct actaaccaga tcaagcagca acaaaccagc cagcctccca aattttaggc
     2701 acaaattaaa aacaacaaaa caaaacaaca aaaaaaaaca acaaaaccaa aaaaagaaaa
     2761 aaaaagaaaa aaacacatta gtttgtaaag tttaaaggac ttttcctttt tcctgtacag
     2821 atttgattag tatgaagtca ggcatctaag aaaacaactg tatttgcatt cccaggtggg
     2881 tggatggatg ctgccgcaga tcacattgca aacagagaaa gcaagcagac acttgatttc
     2941 agcctgtgtc cagctacagg atcaatcttt aactctgcag tctcctcagg cattgtgacc
     3001 cgggatgcag tttgcagctt tagtgccttt ctttgccttt taaatcgcca cgaatcactg
     3061 atggctattt agtggcccta caatgctgca acacatcggc ttgcgtttta gtcttagtta
     3121 tttgtttctt gggtaattgt ttgtgtcagc tgttagtggt gaaactgggc tctagtgaac
     3181 cttttattta tgaagtctaa cttagtgttc acgtggctag tggcaagcat tttacagtga
     3241 tcacccaatt taatcttttg tatatttttt agaaatgcca agagccttac taaactggaa
     3301 cagatttatg atatagtgat gatttaggta gatgttagtc ttgaagctct tattttgtgt
     3361 gcaactgatt atacaaacat attaaccaag cattattaca cacataatat ctataactag
     3421 gactttgata actgttatat aaagtgtgta aaatttgtat gaataaattt ttgtaaacaa
//
