LOCUS       NM_153397               2447 bp    mRNA    linear   ROD 04-AUG-2023
DEFINITION  Mus musculus a disintegrin and metallopeptidase domain 32 (Adam32),
            transcript variant 1, mRNA.
ACCESSION   NM_153397
VERSION     NM_153397.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Lee S, Hong SH and Cho C.
  TITLE     Normal fertility in male mice lacking ADAM32 with testis-specific
            expression
  JOURNAL   Reprod Biol 20 (4), 589-594 (2020)
   PUBMED   32972883
  REMARK    GeneRIF: Normal fertility in male mice lacking ADAM32 with
            testis-specific expression.
REFERENCE   2  (bases 1 to 2447)
  AUTHORS   Koscielny G, Yaikhom G, Iyer V, Meehan TF, Morgan H,
            Atienza-Herrero J, Blake A, Chen CK, Easty R, Di Fenza A, Fiegel T,
            Grifiths M, Horne A, Karp NA, Kurbatova N, Mason JC, Matthews P,
            Oakley DJ, Qazi A, Regnart J, Retha A, Santos LA, Sneddon DJ,
            Warren J, Westerberg H, Wilson RJ, Melvin DG, Smedley D, Brown SD,
            Flicek P, Skarnes WC, Mallon AM and Parkinson H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   3  (bases 1 to 2447)
  AUTHORS   Grayson P and Civetta A.
  TITLE     Positive selection in the adhesion domain of Mus sperm Adam genes
            through gene duplications and function-driven gene complex
            formations
  JOURNAL   BMC Evol Biol 13, 217 (2013)
   PUBMED   24079728
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2447)
  AUTHORS   Kim T, Oh J, Woo JM, Choi E, Im SH, Yoo YJ, Kim DH, Nishimura H and
            Cho C.
  TITLE     Expression and relationship of male reproductive ADAMs in mouse
  JOURNAL   Biol Reprod 74 (4), 744-750 (2006)
   PUBMED   16407499
REFERENCE   5  (bases 1 to 2447)
  AUTHORS   Choi I, Woo JM, Hong S, Jung YK, Kim DH and Cho C.
  TITLE     Identification and characterization of ADAM32 with
            testis-predominant gene expression
  JOURNAL   Gene 304, 151-162 (2003)
   PUBMED   12568724
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK131749.1, BC060983.1 and
            BX636378.1.
            
            [WARNING] On Dec 12, 2023 this sequence was replaced by
            NM_153397.3.
            
            On May 1, 2009 this sequence version replaced NM_153397.1.
            
            Summary: This gene encodes a member of the disintegrin family of
            membrane-anchored proteins that play a role in diverse biological
            processes such as brain development, fertilization, tumor
            development and inflammation. The encoded protein undergoes
            proteolytic processing to generate a mature polypeptide comprised
            of an metalloprotease, disintegrin and epidermal growth factor-like
            domains. This gene was found to be expressed predominantly in the
            pachytene spermatocytes, where the processed protein is localized
            to the sperm surface. This gene is located in a cluster of other
            disintegrin and metallopeptidase family genes on chromosome 8.
            Alternative splicing results in multiple transcript variants
            encoding different isoforms that may undergo similar processing to
            generate mature protein. [provided by RefSeq, Sep 2015].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            CCDS Note: An upstream translation start codon is selected for this
            CCDS which results in a protein that is 4 aa longer at the
            N-terminal.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC060983.1, AF513715.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849384 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-3                 AK131749.1         1-3
            4-2190              BC060983.1         1-2187
            2191-2447           BX636378.1         6-262               c
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8 13.65 cM"
     gene            1..2447
                     /gene="Adam32"
                     /note="a disintegrin and metallopeptidase domain 32"
                     /db_xref="GeneID:353188"
                     /db_xref="MGI:MGI:2653822"
     exon            1..85
                     /gene="Adam32"
                     /inference="alignment:Splign:2.1.0"
     CDS             10..2274
                     /gene="Adam32"
                     /note="isoform 1 preproprotein is encoded by transcript
                     variant 1; a disintegrin and metalloprotease domain 32;
                     disintegrin and metalloproteinase domain-containing
                     protein 32"
                     /codon_start=1
                     /product="disintegrin and metalloproteinase
                     domain-containing protein 32 isoform 1 preproprotein"
                     /protein_id="NP_700446.2"
                     /db_xref="CCDS:CCDS40300.2"
                     /db_xref="GeneID:353188"
                     /db_xref="MGI:MGI:2653822"
                     /translation="MLGAMLHTLLLLLLAELGALLASGPESQSSFLEIIFPEKIEDKT
                     HSEEQISYIIPINKKQYTVHLQKRYFLTNRFMVYMYNQGSTSFHSPNIPAQCYYQGHI
                     KGYPNSVATLSTCSGLRGFLQFENVSYGIEPLQSAFTSQHIVYKLGNKEKELIFNKNS
                     RNIEMPTNYGILINKKPKSPFKNLFPLYLEMSIVVDKALYDYLGSDSNIVTNKIIEII
                     SLINSVFAQLKVTIVLSSLELWSDKNKIPTVGEADELLHKFLEWKQAYLTLRPHDVAY
                     LFIYNEYPNYMGATYPGKMCTAHYSAGITMYPKDMTLEAFSVILTQMLGLSLGISYDE
                     PEKCYCSESICIMNPRAMQYGGVKSFSNCSLNDFEHFKSNEGAKCLQNKPQMQRTAAA
                     VCGNGKVEGDEICDCGSEAECGPDSCCEPNRCVLKAGRACDSKSPSSTCCKNCQFLPE
                     KHQCRPEKHLYCDIPEVCNGSSGNCPPDVTINNGHVCKESGTICYNGDCPDLDRVCES
                     IYGAGSVNAPFACYEEIQGQNDRFGNCGKDNRNRYVFCGWRNLICGRLICTYPTRMPY
                     NPPNNSTASVIYAFVRDKVCITVDFGSSVKEDPLRVANGATCDLDRICLNGVCVESRF
                     LRDQSKTCSSKCHGNGVCNSHGVCHCNAGYSPPNCQYPTTKRSASLWSGKHDLPMERA
                     SKNQEKKWLLSLYIVLIILASVFLIGTGWKGLKQCGSKEEESMSSESKSEDSTYTYVS
                     RSTSETSSMTSTSS"
     exon            86..150
                     /gene="Adam32"
                     /inference="alignment:Splign:2.1.0"
     exon            151..212
                     /gene="Adam32"
                     /inference="alignment:Splign:2.1.0"
     exon            213..288
                     /gene="Adam32"
                     /inference="alignment:Splign:2.1.0"
     exon            289..365
                     /gene="Adam32"
                     /inference="alignment:Splign:2.1.0"
     exon            366..537
                     /gene="Adam32"
                     /inference="alignment:Splign:2.1.0"
     exon            538..606
                     /gene="Adam32"
                     /inference="alignment:Splign:2.1.0"
     exon            607..678
                     /gene="Adam32"
                     /inference="alignment:Splign:2.1.0"
     exon            679..845
                     /gene="Adam32"
                     /inference="alignment:Splign:2.1.0"
     exon            846..927
                     /gene="Adam32"
                     /inference="alignment:Splign:2.1.0"
     exon            928..1064
                     /gene="Adam32"
                     /inference="alignment:Splign:2.1.0"
     exon            1065..1242
                     /gene="Adam32"
                     /inference="alignment:Splign:2.1.0"
     exon            1243..1347
                     /gene="Adam32"
                     /inference="alignment:Splign:2.1.0"
     exon            1348..1546
                     /gene="Adam32"
                     /inference="alignment:Splign:2.1.0"
     exon            1547..1655
                     /gene="Adam32"
                     /inference="alignment:Splign:2.1.0"
     exon            1656..1845
                     /gene="Adam32"
                     /inference="alignment:Splign:2.1.0"
     exon            1846..1929
                     /gene="Adam32"
                     /inference="alignment:Splign:2.1.0"
     exon            1930..2035
                     /gene="Adam32"
                     /inference="alignment:Splign:2.1.0"
     exon            2036..2192
                     /gene="Adam32"
                     /inference="alignment:Splign:2.1.0"
     exon            2193..2231
                     /gene="Adam32"
                     /inference="alignment:Splign:2.1.0"
     exon            2232..2270
                     /gene="Adam32"
                     /inference="alignment:Splign:2.1.0"
     exon            2271..2447
                     /gene="Adam32"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2426..2431
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Adam32"
     polyA_site      2447
                     /gene="Adam32"
ORIGIN      
        1 ggagacgcca tgctgggcgc catgctgcac acactgctgc tgctgctgct ggccgagctg
       61 ggggccttgc tggcatcagg acccgagtct caaagttcat ttctagaaat catatttcca
      121 gagaaaattg aagataaaac acactcagaa gaacagatat cctatattat tccaataaac
      181 aagaagcagt acactgtgca cctccagaaa agatattttt taacgaatcg ttttatggtt
      241 tatatgtata atcaaggatc tacaagtttt cattctccaa acattccggc tcagtgctat
      301 tatcaaggac acatcaaagg ctacccgaac tcagtcgcca ccctcagcac atgctctgga
      361 ctgagagggt tcctgcagtt tgagaatgtg tcctacggaa ttgagcctct gcaatctgca
      421 tttacatctc aacacattgt ttataagcta gggaacaaag agaaggagtt gatatttaac
      481 aaaaacagca gaaacataga gatgcctaca aactatggca ttttaatcaa caaaaagcca
      541 aaatcacctt ttaaaaactt gtttcccctc tatctagaga tgagtattgt ggtggacaaa
      601 gcgctgtatg attacttggg ctctgacagc aatattgtaa caaataaaat cattgaaatt
      661 atcagtctta taaattcagt gtttgcccaa ctcaaggtta ctatcgtgtt gtcctcattg
      721 gagttgtggt cagataagaa taagattcct acagttggtg aagcagatga attattgcat
      781 aaatttctag aatggaagca ggcctacctt accctaagac ctcatgatgt tgcatatcta
      841 ttcatttata acgagtaccc aaattacatg ggagcaacgt atcctgggaa gatgtgtact
      901 gcccactact ctgcaggaat cacaatgtac cccaaggata tgaccttgga ggccttttcg
      961 gttattctca cccaaatgct ggggctcagt ctggggatat cgtatgatga gcctgagaaa
     1021 tgctactgct cggaatccat ctgcatcatg aatcccaggg cgatgcaata tggaggtgtg
     1081 aagtcattta gcaattgcag tttgaatgac tttgaacatt ttaaatcgaa cgagggtgcc
     1141 aaatgtctgc agaataagcc acagatgcaa aggactgcag cagcggtctg cggaaatggc
     1201 aaggtggagg gtgatgaaat ttgtgactgt ggctctgaag cagaatgtgg gcctgatagc
     1261 tgttgtgaac ctaacagatg tgttctgaaa gcaggaagag cttgtgacag taagtctccc
     1321 tcatcaacat gctgcaaaaa ttgtcaattt ttaccagaaa aacatcaatg taggccggaa
     1381 aagcatctat actgtgacat tcctgaagtc tgcaatggga gctcaggaaa ttgtccacct
     1441 gatgtaacta taaacaatgg gcatgtttgc aaagaaagcg ggactatctg ttataatgga
     1501 gactgtccgg acctggacag ggtgtgcgag tcaatatatg gagcaggttc agtgaatgct
     1561 ccatttgcct gctatgaaga aatccagggc caaaatgata ggtttggcaa ctgtggcaaa
     1621 gacaaccgaa acagatatgt gttctgtgga tggaggaatc tcatatgtgg gagattgatt
     1681 tgtacgtacc ctacccgaat gccttacaac ccacccaata acagtacagc ttctgtgatc
     1741 tatgcttttg tgcgagacaa agtgtgtata actgtggact ttggatcgag tgtcaaagaa
     1801 gatccactca gggttgctaa tggtgctaca tgtgatcttg ataggatctg cttaaatggc
     1861 gtgtgcgtag aatcgagatt tcttagggat caatcaaaaa cgtgttcatc caagtgccac
     1921 gggaacggag tgtgcaattc tcatggtgtc tgccactgca atgccggcta ctcacctccc
     1981 aactgtcaat atcctacgac aaaaaggtca gcatcgctgt ggtctggcaa gcacgatctg
     2041 cccatggaaa gagcatctaa gaaccaagaa aagaagtggc ttttaagttt gtacattgtc
     2101 ttaattatcc tcgcttcagt gttcctcata ggcacagggt ggaaaggctt gaaacagtgc
     2161 ggttccaagg aagaggagtc catgagtagc gaaagcaaat cagaagacag cacttacacg
     2221 tatgtcagca gatcaacttc tgagacaagc agtatgacat ctacgagcag ttaattgcta
     2281 ccccttcatc atcagcagca gcagcaacag gaggaagagg aagaggaaga aggggaggag
     2341 gaggaggaga aggaagatgg cacccagggg ctcactgaga aatgaagagt ccacgttctt
     2401 cccttggtca cagctaaaag gagccaataa accaaaagtg gaccaat
//
