LOCUS       NM_010086               2887 bp    mRNA    linear   ROD 01-JUN-2024
DEFINITION  Mus musculus ADAM metallopeptidase domain 24 (Adam24), mRNA.
ACCESSION   NM_010086
VERSION     NM_010086.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2887)
  AUTHORS   Grayson,P. and Civetta,A.
  TITLE     Positive selection in the adhesion domain of Mus sperm Adam genes
            through gene duplications and function-driven gene complex
            formations
  JOURNAL   BMC Evol Biol 13, 217 (2013)
   PUBMED   24079728
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2887)
  AUTHORS   Zhu,G.Z., Gupta,S., Myles,D.G. and Primakoff,P.
  TITLE     Testase 1 (ADAM 24) a sperm surface metalloprotease is required for
            normal fertility in mice
  JOURNAL   Mol Reprod Dev 76 (11), 1106-1114 (2009)
   PUBMED   19670298
  REMARK    GeneRIF: Findings suggest that testase 1 is a sperm plasma membrane
            component which contributes to the prevention of polyspermy at the
            level of the oocyte plasma membrane.
REFERENCE   3  (bases 1 to 2887)
  AUTHORS   Combes,A.N., Lesieur,E., Harley,V.R., Sinclair,A.H., Little,M.H.,
            Wilhelm,D. and Koopman,P.
  TITLE     Three-dimensional visualization of testis cord morphogenesis, a
            novel tubulogenic mechanism in development
  JOURNAL   Dev Dyn 238 (5), 1033-1041 (2009)
   PUBMED   19334288
REFERENCE   4  (bases 1 to 2887)
  AUTHORS   Han,C., Choi,E., Park,I., Lee,B., Jin,S., Kim,D.H., Nishimura,H.
            and Cho,C.
  TITLE     Comprehensive analysis of reproductive ADAMs: relationship of ADAM4
            and ADAM6 with an ADAM complex required for fertilization in mice
  JOURNAL   Biol Reprod 80 (5), 1001-1008 (2009)
   PUBMED   19129510
REFERENCE   5  (bases 1 to 2887)
  AUTHORS   Zhu,G.Z., Myles,D.G. and Primakoff,P.
  TITLE     Testase 1 (ADAM 24) a plasma membrane-anchored sperm protease
            implicated in sperm function during epididymal maturation or
            fertilization
  JOURNAL   J Cell Sci 114 (Pt 9), 1787-1794 (2001)
   PUBMED   11309208
REFERENCE   6  (bases 1 to 2887)
  AUTHORS   Zhu,G.Z., Lin,Y., Myles,D.G. and Primakoff,P.
  TITLE     Identification of four novel ADAMs with potential roles in
            spermatogenesis and fertilization
  JOURNAL   Gene 234 (2), 227-237 (1999)
   PUBMED   10395895
REFERENCE   7  (bases 1 to 2887)
  AUTHORS   Weskamp,G. and Blobel,C.P.
  TITLE     A family of cellular proteins related to snake venom disintegrins
  JOURNAL   Proc Natl Acad Sci U S A 91 (7), 2748-2751 (1994)
   PUBMED   8146185
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BB616396.1, AK029528.1 and
            AK133199.1.
            
            On May 12, 2016 this sequence version replaced NM_010086.4.
            
            Summary: This gene encodes a member of a disintegrin and
            metalloprotease (ADAM) family of endoproteases that play important
            roles in various biological processes including cell signaling,
            adhesion and migration. This gene is expressed in a regulated
            fashion during spermatogenesis. The encoded preproprotein undergoes
            proteolytic processing to generate a mature, functional
            metalloprotease enzyme. Male mice lacking the encoded protein
            exhibit reduced fertility due to the higher incidence of
            polyspermic embryos. This gene is located adjacent to other ADAM
            genes on chromosome 8. [provided by RefSeq, May 2016].
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK029528.1, BB614583.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849384 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-17                BB616396.1         1-17
            18-2350             AK029528.1         2-2334
            2351-2812           AK133199.1         2350-2811
            2813-2887           AK029528.1         2797-2871
FEATURES             Location/Qualifiers
     source          1..2887
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8 23.89 cM"
     gene            1..2887
                     /gene="Adam24"
                     /gene_synonym="Dtgn5"
                     /note="ADAM metallopeptidase domain 24"
                     /db_xref="GeneID:13526"
                     /db_xref="MGI:MGI:105984"
     exon            1..46
                     /gene="Adam24"
                     /gene_synonym="Dtgn5"
                     /inference="alignment:Splign:2.1.0"
     exon            47..2887
                     /gene="Adam24"
                     /gene_synonym="Dtgn5"
                     /inference="alignment:Splign:2.1.0"
     CDS             183..2468
                     /gene="Adam24"
                     /gene_synonym="Dtgn5"
                     /note="a disintegrin and metalloproteinase domain 24
                     (testase 1); disintegrin and metalloproteinase
                     domain-containing protein 24; disintegrin 5; a disintegrin
                     and metallopeptidase domain 24 (testase 1)"
                     /codon_start=1
                     /product="disintegrin and metalloproteinase
                     domain-containing protein 24 preproprotein"
                     /protein_id="NP_034216.3"
                     /db_xref="CCDS:CCDS22255.1"
                     /db_xref="GeneID:13526"
                     /db_xref="MGI:MGI:105984"
                     /translation="MVAMSEALVHARITLLQAWLRMLLFSSVWPPTWCAEYKGPPETV
                     KPLRVIVSSKDMSLAGWMSYSLYFGGQRHIISMKSKNFLESRQLPVFTYNDQGVLFED
                     RPFVQNDCYYLGFVDGDLESMAALTTCFGGFQGILQINDTAYEIKPKSPSSTFEHLLY
                     KIDSEKTQLRPMRCGLTDEEIAGQVRLQENGKSTRMQSIYGSWWSHGLYIKLALVIDH
                     EQYLYRKKNTSLVIRDVLSIMQGINLFLLSVDINVVLLGLTIWTNGNPIPVQDIYALL
                     PAFCTWKGTNLDSQIPYDIAHLFVNYTFSNYFGIAYVGTVCDKTFGCGIDSIAEDDFL
                     TIGHIVAHEIGHNLGMSHDGILCTCGEESCLMSATMDSSQKLSNCSYEVLWAHMINKS
                     CIHREPRPSDIFQLKVCGNGIVEEGEQCDCGSSENCRRNRCCMPSCTLRSKAKCDTGL
                     CCNRKCQIQPSGTLCRARENECDLPEWCNGTSHECPEDLFVQDGTSCPGDGYCYEKRC
                     NSHDVHCQRVFGQLAMKASDSCYKELNTRGDRFGNCGFINNEYVRCEISDILCGRIQC
                     DKVGTLPILQNHYTIHWTHFNSVSCWSTDYHLGMKIADLGDIKDGTNCGPQHVCIARK
                     CVNKPSWVNDCTPETCNMKGVCNNKQHCHCDVGWSPPNCQETGTGGSIDSGSPGNEVY
                     EDEVVSKKDAPEKPNVIIWLLPIICVAVVLSVLFCLSGATKKSREAAASQPAEERVKP
                     PYEGAEPSYETVKPPDEWANP"
     regulatory      2794..2799
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Adam24"
                     /gene_synonym="Dtgn5"
                     /note="hexamer: AATAAA"
     polyA_site      2815
                     /gene="Adam24"
                     /gene_synonym="Dtgn5"
                     /note="major polyA site"
ORIGIN      
        1 gagtcatgaa taagactaca ccaagatatt atcactactt ctcctgtcct cctgccctga
       61 tggatccact tttccagaag acagactatt aacggtcagt gctgcatggg attaagacaa
      121 aggaagtaca tgggcaacag gaccacatcc tatcctcctt catttgctct ctctggtagg
      181 acatggtggc tatgagtgag gctctggttc atgcaaggat cactctcctt caggcatggt
      241 tgaggatgct gctcttttct tctgtatggc ccccgacttg gtgtgctgaa tacaaaggcc
      301 ctccagagac agtgaaaccc ttgagggtaa ttgtctctag taaagacatg agtcttgcag
      361 gctggatgtc ctatagtctg tactttggag gccagagaca cattatctcc atgaaatcca
      421 agaatttttt ggaatccaga cagctccctg tgttcacata caatgaccaa ggtgtcctct
      481 ttgaggacag accttttgtc caaaatgact gttattatct tggttttgtg gatggagatc
      541 tagaatccat ggctgctctt accacctgtt ttgggggctt tcaaggaata ttacagataa
      601 atgacacagc ttatgaaatt aagcccaaga gcccctcttc cacatttgaa catctgcttt
      661 acaagataga cagtgagaaa actcaattac gccccatgag atgtggatta acagatgaag
      721 aaatagcagg gcaagtgaga cttcaagaaa atggtaagtc cactagaatg caaagcatct
      781 atgggtcatg gtggtctcat ggattgtata ttaaactggc attggttata gaccatgaac
      841 aataccttta tcgaaaaaaa aatacttctc ttgtgataag agatgtatta agcattatgc
      901 agggaataaa tctcttctta ctttcagtgg atattaatgt ggttttactt ggacttacta
      961 tctggactaa tggaaatcct ataccagtgc aagatatata tgctcttttg ccagcatttt
     1021 gtacgtggaa gggaacaaac cttgattctc agataccata tgatattgca catctctttg
     1081 tgaattatac ttttagtaac tattttggca tagcctatgt aggaactgta tgtgataaga
     1141 catttggttg tggaattgat agtatcgctg aagatgattt ccttaccatt ggacatattg
     1201 tggcacatga gataggtcac aatttgggca tgtcacatga tgggatactt tgtacttgcg
     1261 gagaagaatc atgtttaatg tctgctacaa tggatagttc ccaaaaactc agcaactgta
     1321 gttatgaagt cttgtgggca cacatgatca ataaaagctg catacataga gagcccagac
     1381 cttctgatat tttccaattg aaggtctgtg ggaatggtat agttgaagaa ggtgagcagt
     1441 gtgactgtgg aagctctgaa aactgtagac gtaatcgctg ttgtatgcct agctgtactc
     1501 tgaggtctaa agctaaatgt gatactggac tatgttgtaa ccgcaaatgc caaatccagc
     1561 catctggcac tctgtgtaga gctcgggaga atgaatgtga ccttccagaa tggtgcaatg
     1621 gaacctcaca tgagtgccct gaagacttgt ttgtacagga tgggacctct tgccctggtg
     1681 atggctactg ctatgaaaaa agatgtaaca gccacgatgt ccactgtcag agagtttttg
     1741 gccagcttgc catgaaagca tcggatagct gttacaagga actcaatact cgtggtgatc
     1801 gttttggtaa ctgtggcttc ataaacaacg agtatgtgag atgtgagatc tcagacatcc
     1861 tctgtgggag gattcaatgt gacaaagtgg gaacacttcc tatcctgcag aatcattata
     1921 ctattcactg gactcatttt aatagtgttt cctgctggag tactgactac catttgggga
     1981 tgaaaattgc tgacctcggt gacataaaag atggcacaaa ctgtggtcca cagcatgtgt
     2041 gcattgccag aaagtgtgtt aataagccaa gttgggtaaa tgattgtaca ccagagacct
     2101 gcaacatgaa aggagtctgc aataataagc agcactgcca ttgtgatgtt ggctggagcc
     2161 caccaaactg ccaggaaact ggcaccggag gaagtattga cagtggttct cctggaaacg
     2221 aagtttatga agatgaagtt gtgagcaaga aggatgcccc agaaaaacct aacgtcataa
     2281 tttggttatt acctattatt tgtgtggctg tagttttatc tgtattgttt tgcttgtctg
     2341 gggccactaa aaaatcacgt gaagcagcag caagtcaacc agcagaggag agagttaaac
     2401 caccatatga aggggctgaa ccctcatatg agacagttaa accaccggat gaatgggcta
     2461 acccctagat aaagaacaaa aggattaaac caccagatga ggaataagag actaaatcat
     2521 aagacaagaa agaagaggct aaaccatcca atgagaaaag aggctaagcc catccatcat
     2581 atgagagtag cagctccttg tcaacagctt cacttcttca acttcaacca acacagaata
     2641 gaaaaatcta atgagttgtc aaaataatct ttaagattta atgcaattta ccacacagca
     2701 tcctggtgtg agagatccta atgtgtatta taaaccactg atagaagatc ccagcaatct
     2761 ataataggtc aggaaaatac acatttatca ttcaataaat atgagaacta ccatattccc
     2821 acttttcata atctgaagaa atttaggtaa cttcttactt ttctaattaa aaacttttat
     2881 ggattgc
//
