LOCUS       NM_001272052            4430 bp    mRNA    linear   ROD 14-FEB-2025
DEFINITION  Mus musculus adenosine deaminase (Ada), transcript variant 1, mRNA.
ACCESSION   NM_001272052
VERSION     NM_001272052.2
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4430)
  AUTHORS   Ding,Z., Ge,W., Xu,X., Xu,X., Sun,Q., Xu,X. and Zhang,J.
  TITLE     A crucial role of adenosine deaminase in regulating gluconeogenesis
            in mice
  JOURNAL   J Biol Chem 300 (7), 107425 (2024)
   PUBMED   38823639
  REMARK    GeneRIF: A crucial role of adenosine deaminase in regulating
            gluconeogenesis in mice.
REFERENCE   2  (bases 1 to 4430)
  AUTHORS   Moretti,F.A., Giardino,G., Attenborough,T.C.H., Gkazi,A.S.,
            Margetts,B.K., la Marca,G., Fairbanks,L., Crompton,T. and
            Gaspar,H.B.
  TITLE     Metabolite and thymocyte development defects in ADA-SCID mice
            receiving enzyme replacement therapy
  JOURNAL   Sci Rep 11 (1), 23221 (2021)
   PUBMED   34853379
  REMARK    Erratum:[Sci Rep. 2021 Dec 15;11(1):24342. doi:
            10.1038/s41598-021-03903-7.. PMID: 34912004]
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4430)
  AUTHORS   Collum,S.D., Molina,J.G., Hanmandlu,A., Bi,W., Pedroza,M.,
            Mertens,T.C.J., Wareing,N., Wei,W., Wilson,C., Sun,W., Rajadas,J.,
            Bollyky,P.L., Philip,K.M., Ren,D., Thandavarayan,R.A.,
            Bruckner,B.A., Xia,Y., Blackburn,M.R. and Karmouty-Quintana,H.
  TITLE     Adenosine and hyaluronan promote lung fibrosis and pulmonary
            hypertension in combined pulmonary fibrosis and emphysema
  JOURNAL   Dis Model Mech 12 (5) (2019)
   PUBMED   31036697
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4430)
  AUTHORS   Xu,X., Negandhi,J., Min,W., Tsui,M., Post,M., Harrison,R.V. and
            Grunebaum,E.
  TITLE     Early Enzyme Replacement Therapy Improves Hearing and Immune
            Defects in Adenosine Deaminase Deficient-Mice
  JOURNAL   Front Immunol 10, 416 (2019)
   PUBMED   30918508
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4430)
  AUTHORS   Kutryb-Zajac,B., Mierzejewska,P., Sucajtys-Szulc,E., Bulinska,A.,
            Zabielska,M.A., Jablonska,P., Serocki,M., Koszalka,P.,
            Milczarek,R., Jasztal,A., Bartoszewski,R., Chlopicki,S.,
            Slominska,E.M. and Smolenski,R.T.
  TITLE     Inhibition of LPS-stimulated ecto-adenosine deaminase attenuates
            endothelial cell activation
  JOURNAL   J Mol Cell Cardiol 128, 62-76 (2019)
   PUBMED   30641086
  REMARK    GeneRIF: Inhibition of eADA blocked endothelial activation
            suggesting a crucial role of this enzyme in the control of vascular
            inflammation. This supports the concept of eADA targeted vascular
            protection therapy
REFERENCE   6  (bases 1 to 4430)
  AUTHORS   Winston,J.H., Hanten,G.R., Overbeek,P.A. and Kellems,R.E.
  TITLE     5' flanking sequences of the murine adenosine deaminase gene direct
            expression of a reporter gene to specific prenatal and postnatal
            tissues in transgenic mice
  JOURNAL   J Biol Chem 267 (19), 13472-13479 (1992)
   PUBMED   1618849
REFERENCE   7  (bases 1 to 4430)
  AUTHORS   Avraham,K.B., Prezioso,V.R., Chen,W.S., Lai,E., Sladek,F.M.,
            Zhong,W., Darnell,J.E. Jr., Jenkins,N.A. and Copeland,N.G.
  TITLE     Murine chromosomal location of four hepatocyte-enriched
            transcription factors: HNF-3 alpha, HNF-3 beta, HNF-3 gamma, and
            HNF-4
  JOURNAL   Genomics 13 (2), 264-268 (1992)
   PUBMED   1612587
REFERENCE   8  (bases 1 to 4430)
  AUTHORS   Wilkie,T.M., Gilbert,D.J., Olsen,A.S., Chen,X.N., Amatruda,T.T.,
            Korenberg,J.R., Trask,B.J., de Jong,P., Reed,R.R., Simon,M.I. et
            al.
  TITLE     Evolution of the mammalian G protein alpha subunit multigene family
  JOURNAL   Nat Genet 1 (2), 85-91 (1992)
   PUBMED   1302014
  REMARK    Review article
REFERENCE   9  (bases 1 to 4430)
  AUTHORS   Pilz,A., Le Tissier,P., Moseley,H., Peters,J. and Abbott,C.
  TITLE     Mapping of the structural gene for S-adenosyl homocysteine
            hydrolase to mouse chromosome 2, and related sequences to
            chromosomes 8 and X
  JOURNAL   Mamm Genome 3 (11), 633-636 (1992)
   PUBMED   1450513
REFERENCE   10 (bases 1 to 4430)
  AUTHORS   Snyder,F.F., Cruikshank,M.K. and Seegmiller,J.E.
  TITLE     A comparison of purine metabolism and nucleotide pools in normal
            and hypoxanthine-guanine phosphoribosyltransferase-deficient
            neuroblastoma cells
  JOURNAL   Biochim Biophys Acta 543 (4), 556-569 (1978)
   PUBMED   718989
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL591490.12.
            
            On Feb 21, 2023 this sequence version replaced NM_001272052.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR13862062.191653.1,
                                           SRR5189676.118512.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849380, SAMN01164134
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-119               AL591490.12        124928-125046       c
            120-181             AL591490.12        113032-113093       c
            182-304             AL591490.12        110218-110340       c
            305-448             AL591490.12        107723-107866       c
            449-564             AL591490.12        107126-107241       c
            565-692             AL591490.12        105974-106101       c
            693-764             AL591490.12        105350-105421       c
            765-866             AL591490.12        105167-105268       c
            867-931             AL591490.12        104881-104945       c
            932-1061            AL591490.12        102986-103115       c
            1062-1161           AL591490.12        102413-102512       c
            1162-4430           AL591490.12        98652-101920        c
FEATURES             Location/Qualifiers
     source          1..4430
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 84.44 cM"
     gene            1..4430
                     /gene="Ada"
                     /note="adenosine deaminase"
                     /db_xref="GeneID:11486"
                     /db_xref="MGI:MGI:87916"
     exon            1..119
                     /gene="Ada"
                     /inference="alignment:Splign:2.1.0"
     CDS             87..1145
                     /gene="Ada"
                     /EC_number="3.5.4.4"
                     /note="isoform 1 is encoded by transcript variant 1;
                     adenosine aminohydrolase"
                     /codon_start=1
                     /product="adenosine deaminase isoform 1"
                     /protein_id="NP_001258981.1"
                     /db_xref="CCDS:CCDS17015.1"
                     /db_xref="GeneID:11486"
                     /db_xref="MGI:MGI:87916"
                     /translation="MAQTPAFNKPKVELHVHLDGAIKPETILYFGKKRGIALPADTVE
                     ELRNIIGMDKPLSLPGFLAKFDYYMPVIAGCREAIKRIAYEFVEMKAKEGVVYVEVRY
                     SPHLLANSKVDPMPWNQTEGDVTPDDVVDLVNQGLQEGEQAFGIKVRSILCCMRHQPS
                     WSLEVLELCKKYNQKTVVAMDLAGDETIEGSSLFPGHVEAYEGAVKNGIHRTVHAGEV
                     GSPEVVREAVDILKTERVGHGYHTIEDEALYNRLLKENMHFEVCPWSSYLTGAWDPKT
                     THAVVRFKNDKANYSLNTDDPLIFKSTLDTDYQMTKKDMGFTEEEFKRLNINAAKSSF
                     LPEEEKKELLERLYREYQ"
     exon            120..181
                     /gene="Ada"
                     /inference="alignment:Splign:2.1.0"
     exon            182..304
                     /gene="Ada"
                     /inference="alignment:Splign:2.1.0"
     exon            305..448
                     /gene="Ada"
                     /inference="alignment:Splign:2.1.0"
     exon            449..564
                     /gene="Ada"
                     /inference="alignment:Splign:2.1.0"
     exon            565..692
                     /gene="Ada"
                     /inference="alignment:Splign:2.1.0"
     exon            693..764
                     /gene="Ada"
                     /inference="alignment:Splign:2.1.0"
     exon            765..866
                     /gene="Ada"
                     /inference="alignment:Splign:2.1.0"
     exon            867..931
                     /gene="Ada"
                     /inference="alignment:Splign:2.1.0"
     exon            932..1061
                     /gene="Ada"
                     /inference="alignment:Splign:2.1.0"
     exon            1062..1161
                     /gene="Ada"
                     /inference="alignment:Splign:2.1.0"
     exon            1162..4430
                     /gene="Ada"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1377..1382
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ada"
                     /note="hexamer: AATAAA"
     polyA_site      1399
                     /gene="Ada"
                     /note="major polyA site"
     regulatory      4399..4404
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ada"
                     /note="hexamer: AATAAA"
     polyA_site      4430
                     /gene="Ada"
ORIGIN      
        1 gagagccatc gggaagcgac cctgccagcg agccaacgca gacccagaga gcttcggcgg
       61 agagaaccgg gaacacgctc ggaaccatgg cccagacacc cgcattcaac aaacccaaag
      121 tagagttaca cgtccacctg gatggagcca tcaagccaga aaccatctta tactttggca
      181 agaagagagg catcgccctc ccggcagata cagtggagga gctgcgcaac attatcggca
      241 tggacaagcc cctctcgctc ccaggcttcc tggccaagtt tgactactac atgcctgtga
      301 ttgcgggctg cagagaggcc atcaagagga tcgcctacga gtttgtggag atgaaggcaa
      361 aggagggcgt ggtctatgtg gaagtgcgct atagcccaca cctgctggcc aattccaagg
      421 tggacccaat gccctggaac cagactgaag gggacgtcac ccctgatgac gttgtggatc
      481 ttgtgaacca gggcctgcag gagggagagc aagcatttgg catcaaggtc cggtccattc
      541 tgtgctgcat gcgccaccag cccagctggt cccttgaggt gttggagctg tgtaagaagt
      601 acaatcagaa gaccgtggtg gctatggact tggctgggga tgagaccatt gaaggaagta
      661 gcctcttccc aggccacgtg gaagcctatg agggcgcagt aaagaatggc attcatcgga
      721 ccgtccacgc tggcgaggtg ggctctcctg aggttgtgcg tgaggctgtg gacatcctca
      781 agacagagag ggtgggacat ggttatcaca ccatcgagga tgaagctctc tacaacagac
      841 tactgaaaga aaacatgcac tttgaggtct gcccctggtc cagctacctc acaggcgcct
      901 gggatcccaa aacgacgcat gcggttgttc gcttcaagaa tgataaggcc aactactcac
      961 tcaacacaga cgaccccctc atcttcaagt ccaccctaga cactgactac cagatgacca
     1021 agaaagacat gggcttcact gaggaggagt tcaagcgact gaacatcaac gcagcgaagt
     1081 caagcttcct cccagaggaa gagaagaagg aacttctgga acggctctac agagaatacc
     1141 aatagccacc acagactgac gcagggcggg tcccctgaag atggcaaggc cacttctctg
     1201 agcctcatcc tgtggataaa gtctttacaa ctctgacata ttgaccttca ttccttccag
     1261 accttggaga ggccaggtct gtcctctgat tggatatcct ggctaggtcc caggggactt
     1321 gacaatcatg cacatgaatt gaaaaccttc cttctaaagc taaaattatg gtgttcaata
     1381 aagcagctgg tgactggtat cttgcagcac atggtgaata tggtctcggg gctgctggct
     1441 aggatgctaa gaaaggagga gccctgggcc ctacgctgag tgtcaggctg gggagccagg
     1501 gtctctttcc tgcagaagcg attctttccc agaggggctg ttggagcaga tgctcctgaa
     1561 ctctccgccc ctttaaccag tcctttggat ttatttttat tatttttaaa tatttaatta
     1621 tgtttatgta tatgggtgtt ttgcctgctt gtatgtatat gcatcatgtg tgcctggtgc
     1681 cagaggtcag aaaagggtac cacctcccct ggtactggaa tttaggtagt tctaacccac
     1741 catgtgccca tgtgcccacc agtggacagc aagtgagagc cgactctctc ttcctaccct
     1801 gtcagcccca gggatgaact caggctgcca ggctgggtag taagtctcta cccactgagc
     1861 atctcacagg cccttttcag gctgtggcag gtgtgggctg tgggtgggtg aggtgaggaa
     1921 ggactcatga gatgccacat gaggggttgt gctggtcacc acaggataga gggcagcagt
     1981 ggggccctgc agggtgagga tgtggggatg cagagcccag ctgtgcagtt gatagttaaa
     2041 agaaaagatc ataagccagt cacacaaacc accagcattt atttcctggg cctaagtatc
     2101 agtctcttgg tacaaagggc aaagaattgg tcagttagtt ttgagtccaa atcctttccc
     2161 aaaactggat ctgtgaggct ccatgaggaa acttagagct gcatagatca tcagaactga
     2221 gctttaaacg gctttcaaaa caaaaccaaa accaaaaacc aaaacagaag aaaagtgatt
     2281 gtcagctccc ctctctctct gaaaaagtta tgtttttaaa aaatgtaaaa gaggctttaa
     2341 aaaagggcta ggaagatctt cctgacagac aggctggctg gagggtgagt ggtgacagga
     2401 cagtccctgt ccaggtggcg gctgctcacc taggggcagg gcctggctcg agccccgcct
     2461 agctgaataa ataacatctc atggtgctgg tgtcttcaga gtgaacctgg gcaccgtggg
     2521 gaggtgggga agggtggagc ctgtgctgtg ggtggaggac ttggctggag tgggggccac
     2581 agaggactcc atgaggctct cagtccgcct ggctggccag ggctccagtc cgagaggtac
     2641 agcaggtctc ttccagcctt gcgaccacag gcagactcag gatgaggtgt tcgcatcact
     2701 gctctcaggc tggctgctgg cttccttgtc gggggtactt ccctctgcct gtccttctgc
     2761 aggggaaaag aagagtgcat tttcttcttt cacccattta catgtgagaa gcacaggggg
     2821 cctggaagga gcagccacac tgatgaccgg ggccagcctt ggaagtaggc taatgggttc
     2881 gttagggagg gcgtggccaa ggccggagag acatttaacc tgctctcagg tctcctttta
     2941 ccatgggaag gctgccaggg ctgctggtat actaaaactc agtacaggtc aggactacag
     3001 acctgctgat cagcatgtcc tctctagagg gagaatgtta agttcccagc aggacgtatg
     3061 tgcttagcaa atgggagttt cctcaaacac catataggag ctgaaaccag tgggcagctc
     3121 atgcgtgccc actgtggcct gcacatccta aggtagagga tacctgactc tgaataccac
     3181 tggcagcatc cagccctgct ctattccagc cagccagaaa catgggctgg aataagggac
     3241 agcagcaggc tggggagcca cctggcctgg cctccaagtc cctacctgat ctggtcctca
     3301 ctcgaagctt ctctgaagcc ctgatggaag gtgggaccag cagcttctgc cccacccact
     3361 tgctagcagc ttgtattgtt attcagccct ctcagggcca gcatcctcca cagatgctgg
     3421 ttatggacaa aactcaacca gcaatgcagt ctcaagcccc agggatcttg gtgccactga
     3481 aggtgcatgt cattctgcca ttaggcctgt ctttaagtct atgttgagct ctgggtctgg
     3541 aattctgtca aacaatccac acagcaacct gaaggtgtgg ttaaggtcat gacacccata
     3601 aggaaacaag cttagagggg caagatacct gcaaccaaag gggagagcct gcttactgct
     3661 gagccccact tcttcctcta agctgcattc tcctgcattc atagaggcat gttggcagga
     3721 cggcctccca ttgccctaca cagcagtctt tggctctgag tgctcatgga agtaaatagt
     3781 gtggacagaa cttggtactg tggctcaagt atgtgccaat ctgagcctgc ctgtcctgaa
     3841 gcggcctcct ctgaggatgg gggctgaacc aagattcagc cacaaagttc tcctggtctc
     3901 tggaggccta acacccctga ggtactaggt tcatttgtga agcccccgtg aactgagacc
     3961 ctttaaatcc cccattgtct ctgctgttca ctccactgct cgagactaca ggcaaggcac
     4021 tttatagtaa ccaacctggc agggatgaag atatcctgaa gagactctgt ccttcacctt
     4081 ggctgtcaga ctattgaaac attgggcccc caagaaatta tatattccca aggatctcct
     4141 gctgaagaag aaaagcctga gcctgagcca accacgggga cttttctcca ggaaggggtt
     4201 ctgtgacttg ccagtgactg aggatagtgg gccctggaca atgaaggagc agtgatgggc
     4261 agaccacacc ttgtgaggac agcctgggtg tgcacaacgc tggcctctac ttaaacctac
     4321 cctcatggca gagcccaaaa gatgaacttg gagggatcat ctgacctctc tgtccctctt
     4381 cccaatgtgg ccatgctgaa taaattgcct tttgttgttc ttcattttaa
//
