LOCUS       NM_001360548            3869 bp    mRNA    linear   ROD 18-APR-2023
DEFINITION  Mus musculus actinin alpha 4 (Actn4), transcript variant 2, mRNA.
ACCESSION   NM_001360548 XM_006540254
VERSION     NM_001360548.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3869)
  AUTHORS   Broadway-Stringer S, Jiang H, Wadmore K, Hooper C, Douglas G,
            Steeples V, Azad AJ, Singer E, Reyat JS, Galatik F, Ehler E,
            Bennett P, Kalisch-Smith JI, Sparrow DB, Davies B, Djinovic-Carugo
            K, Gautel M, Watkins H and Gehmlich K.
  TITLE     Insights into the Role of a Cardiomyopathy-Causing Genetic Variant
            in ACTN2
  JOURNAL   Cells 12 (5), 721 (2023)
   PUBMED   36899856
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3869)
  AUTHORS   Wu T, Xu Y, Zhang L, Liang Z, Zhou X, Evans SM and Chen J.
  TITLE     Filamin C is Essential for mammalian myocardial integrity
  JOURNAL   PLoS Genet 19 (1), e1010630 (2023)
   PUBMED   36706168
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3869)
  AUTHORS   Tseng CC, Zheng RH, Lin TW, Chou CC, Shih YC, Liang SW and Lee HH.
  TITLE     alpha-Actinin-4 recruits Shp2 into focal adhesions to potentiate
            ROCK2 activation in podocytes
  JOURNAL   Life Sci Alliance 5 (11), e202201557 (2022)
   PUBMED   36096674
  REMARK    GeneRIF: alpha-Actinin-4 recruits Shp2 into focal adhesions to
            potentiate ROCK2 activation in podocytes.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3869)
  AUTHORS   Rudolf MA, Andreeva A, Kim CE, DeNovio AC, Koshar AN, Baker W,
            Cartagena-Rivera AX and Corwin JT.
  TITLE     Stiffening of Circumferential F-Actin Bands Correlates With
            Regenerative Failure and May Act as a Biomechanical Brake in the
            Mammalian Inner Ear
  JOURNAL   Front Cell Neurosci 16, 859882 (2022)
   PUBMED   35602553
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3869)
  AUTHORS   Li Y, Gong W, Liu J, Chen X, Suo Y, Yang H and Gao X.
  TITLE     Angiopoietin-like protein 4 promotes hyperlipidemia-induced renal
            injury by down-regulating the expression of ACTN4
  JOURNAL   Biochem Biophys Res Commun 595, 69-75 (2022)
   PUBMED   35101665
  REMARK    GeneRIF: Angiopoietin-like protein 4 promotes
            hyperlipidemia-induced renal injury by down-regulating the
            expression of ACTN4.
REFERENCE   6  (bases 1 to 3869)
  AUTHORS   Huttelmaier S, Illenberger S, Grosheva I, Rudiger M, Singer RH and
            Jockusch BM.
  TITLE     Raver1, a dual compartment protein, is a ligand for PTB/hnRNPI and
            microfilament attachment proteins
  JOURNAL   J Cell Biol 155 (5), 775-786 (2001)
   PUBMED   11724819
REFERENCE   7  (bases 1 to 3869)
  AUTHORS   Piao Y, Ko NT, Lim MK and Ko MS.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
REFERENCE   8  (bases 1 to 3869)
  AUTHORS   Mills M, Yang N, Weinberger R, Vander Woude DL, Beggs AH, Easteal S
            and North K.
  TITLE     Differential expression of the actin-binding proteins,
            alpha-actinin-2 and -3, in different species: implications for the
            evolution of functional redundancy
  JOURNAL   Hum Mol Genet 10 (13), 1335-1346 (2001)
   PUBMED   11440986
REFERENCE   9  (bases 1 to 3869)
  AUTHORS   Dear TN, Meier NT, Hunn M and Boehm T.
  TITLE     Gene structure, chromosomal localization, and expression pattern of
            Capn12, a new member of the calpain large subunit gene family
  JOURNAL   Genomics 68 (2), 152-160 (2000)
   PUBMED   10964513
REFERENCE   10 (bases 1 to 3869)
  AUTHORS   Araki N, Hatae T, Yamada T and Hirohashi S.
  TITLE     Actinin-4 is preferentially involved in circular ruffling and
            macropinocytosis in mouse macrophages: analysis by fluorescence
            ratio imaging
  JOURNAL   J Cell Sci 113 (Pt 18), 3329-3340 (2000)
   PUBMED   10954430
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC166357.3.
            
            [WARNING] On Apr 26, 2023 this sequence was replaced by
            NM_001360548.2.
            
            On Feb 6, 2018 this sequence version replaced XM_006540254.3.
            
            Transcript Variant: This variant (2) lacks an alternate internal
            exon and contains another alternate internal exon compared to
            variant 1. The resulting isoform (2) has the same length as isoform
            1 but differs at two amino acid positions.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660819.103232.1,
                                           SRR7345562.2537672.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131 [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-314               AC166357.3         21782-22095
            315-429             AC166357.3         65046-65160
            430-549             AC166357.3         65323-65442
            550-636             AC166357.3         67868-67954
            637-724             AC166357.3         68814-68901
            725-803             AC166357.3         70658-70736
            804-885             AC166357.3         71793-71874
            886-971             AC166357.3         74126-74211
            972-1064            AC166357.3         77050-77142
            1065-1295           AC166357.3         78659-78889
            1296-1443           AC166357.3         79426-79573
            1444-1594           AC166357.3         82087-82237
            1595-1703           AC166357.3         85386-85494
            1704-1844           AC166357.3         85635-85775
            1845-2027           AC166357.3         85863-86045
            2028-2162           AC166357.3         86131-86265
            2163-2342           AC166357.3         87059-87238
            2343-2489           AC166357.3         87879-88025
            2490-2570           AC166357.3         88602-88682
            2571-2729           AC166357.3         89455-89613
            2730-3869           AC166357.3         89727-90866
FEATURES             Location/Qualifiers
     source          1..3869
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7"
     gene            1..3869
                     /gene="Actn4"
                     /note="actinin alpha 4"
                     /db_xref="GeneID:60595"
                     /db_xref="MGI:MGI:1890773"
     exon            1..314
                     /gene="Actn4"
                     /inference="alignment:Splign:2.1.0"
     CDS             150..2888
                     /gene="Actn4"
                     /note="isoform 2 is encoded by transcript variant 2;
                     alpha-actinin-4; non-muscle alpha-actinin 4; F-actin
                     cross-linking protein"
                     /codon_start=1
                     /product="alpha-actinin-4 isoform 2"
                     /protein_id="NP_001347477.1"
                     /db_xref="GeneID:60595"
                     /db_xref="MGI:MGI:1890773"
                     /translation="MVDYHAANQAYQYGPNSGGGNGAGGGGSMGDYMAQEDDWDRDLL
                     LDPAWEKQQRKTFTAWCNSHLRKAGTQIENIDEDFRDGLKLMLLLEVISGERLPKPER
                     GKMRVHKINNVNKALDFIASKGVKLVSIGAEEIVDGNAKMTLGMIWTIILRFAIQDIS
                     VEETSAKEGLLLWCQRKTAPYKNVNVQNFHISWKDGLAFNALIHRHRPELIEYDKLRK
                     DDPVTNLNNAFEVAEKYLDIPKMLDAEDIVGTLRPDEKAIMTYVSCFYHAFSGAQKAE
                     TAANRICKVLAVNQENEHLMEDYERLASDLLEWIRRTIPWLEDRVPQKTIQEMQQKLE
                     DFRDYRRVHKPPKVQEKCQLEINFNTLQTKLRLSNRPAFMPSEGRMVSDINNGWQHLE
                     QAEKGYEEWLLNEIRRLERLDHLAEKFRQKASIHEAWTDGKEAMLKQRDYETATLSDI
                     KALIRKHEAFESDLAAHQDRVEQIAAIAQELNELDYYDSHNVNTRCQKICDQWDNLGS
                     LTHSRREALEKTEKQLETIDQLHLEYAKRAAPFNNWMESAMEDLQDMFIVHTIEEIEG
                     LISAHDQFKSTLPDADREREAILAIHKEAQRIAESNHIKLSGSNPYTTVTPQIINSKW
                     EKVQQLVPKRDHALLEEQSKQQSNEHLRRQFASQANMVGPWIQTKMEEIGRISIEMNG
                     TLEDQLSHLKQYERSIVDYKPSLDLLEQQHQLIQEALIFDNKHTNYTMEHIRVGWEQL
                     LTTIARTINEVENQILTRDAKGISQEQMQEFRASFNHFDKDHGGALGPEEFKACLISL
                     GYDVENDRQGDAEFNRIMSVVDPNHSGLVTFQAFIDFMSRETTDTDTADQVIASFKVL
                     AGDKNFITAEELRRELPPDQAEYCIARMAPYQGPDAAPGALDYKSFSTALYGESDL"
     exon            315..429
                     /gene="Actn4"
                     /inference="alignment:Splign:2.1.0"
     exon            430..549
                     /gene="Actn4"
                     /inference="alignment:Splign:2.1.0"
     exon            550..636
                     /gene="Actn4"
                     /inference="alignment:Splign:2.1.0"
     exon            637..724
                     /gene="Actn4"
                     /inference="alignment:Splign:2.1.0"
     exon            725..803
                     /gene="Actn4"
                     /inference="alignment:Splign:2.1.0"
     exon            804..885
                     /gene="Actn4"
                     /inference="alignment:Splign:2.1.0"
     exon            886..971
                     /gene="Actn4"
                     /inference="alignment:Splign:2.1.0"
     exon            972..1064
                     /gene="Actn4"
                     /inference="alignment:Splign:2.1.0"
     exon            1065..1295
                     /gene="Actn4"
                     /inference="alignment:Splign:2.1.0"
     exon            1296..1443
                     /gene="Actn4"
                     /inference="alignment:Splign:2.1.0"
     exon            1444..1594
                     /gene="Actn4"
                     /inference="alignment:Splign:2.1.0"
     exon            1595..1703
                     /gene="Actn4"
                     /inference="alignment:Splign:2.1.0"
     exon            1704..1844
                     /gene="Actn4"
                     /inference="alignment:Splign:2.1.0"
     exon            1845..2027
                     /gene="Actn4"
                     /inference="alignment:Splign:2.1.0"
     exon            2028..2162
                     /gene="Actn4"
                     /inference="alignment:Splign:2.1.0"
     exon            2163..2342
                     /gene="Actn4"
                     /inference="alignment:Splign:2.1.0"
     exon            2343..2489
                     /gene="Actn4"
                     /inference="alignment:Splign:2.1.0"
     exon            2490..2570
                     /gene="Actn4"
                     /inference="alignment:Splign:2.1.0"
     exon            2571..2729
                     /gene="Actn4"
                     /inference="alignment:Splign:2.1.0"
     exon            2730..3869
                     /gene="Actn4"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 agtgcgcagg cgcggcaagt gaactgcgca ggcgtcagtc ttgggcgggc tgaaagcagc
       61 tgaagcggcg gccgagtcgg agcctccggc acaggggcgg gagctgaggc ggtagcggac
      121 cggctggcgg gctagcgaaa ggcggcggaa tggtggacta ccacgcagcg aaccaggcgt
      181 accagtacgg cccgaacagc ggcggcggca atggcgcggg tggcggcgga agcatgggcg
      241 actacatggc ccaggaggat gactgggacc gggacctgct gttggacccg gcctgggaga
      301 agcagcagcg caagaccttc acagcctggt gcaactctca tcttcggaag gctggcactc
      361 agatcgagaa tatcgatgag gacttcaggg atgggctcaa acttatgctt ctgttggagg
      421 tcatttcagg ggagcgtttg cctaagccag agcgggggaa gatgcgagtg cacaagatca
      481 acaatgtaaa caaagccctg gacttcatcg ccagcaaggg agtcaagctg gtgtccatcg
      541 gggcagaaga gattgtggat ggcaatgcaa agatgaccct gggaatgatc tggaccatca
      601 tcctcagatt tgccatccag gacatctctg tggaagagac ctctgctaag gaaggactcc
      661 tcctctggtg ccagagaaag acagccccat ataaaaacgt caacgtccag aacttccata
      721 tcagctggaa ggacgggctg gccttcaatg cactcatcca ccggcacagg cctgagctga
      781 ttgagtatga caagctgcgg aaggatgatc cagtcaccaa cctaaacaat gcatttgaag
      841 tggctgagaa atacctcgat atccccaaga tgttggatgc tgaggatatt gtaggcactc
      901 tgaggccaga tgagaaggcc atcatgactt acgtgtcctg cttctaccac gccttctcgg
      961 gggcccagaa ggctgagacc gctgccaacc ggatctgcaa ggtgctggct gtgaaccagg
     1021 aaaatgagca cctgatggaa gactatgaac gcctggccag tgatcttcta gagtggattc
     1081 ggcgcaccat cccctggctg gaggaccggg tgcctcagaa gaccatccag gaaatgcagc
     1141 agaagctgga ggacttccga gactataggc gtgtgcacaa gccgcccaag gtgcaggaga
     1201 agtgtcagct ggagatcaac ttcaacacac tgcagaccaa gctgcggctc agcaaccggc
     1261 ctgccttcat gccctccgag ggcaggatgg tctccgacat caacaatgga tggcagcacc
     1321 tggagcaggc tgagaagggc tatgaagaat ggctgctgaa tgaaatccgt aggctggaac
     1381 ggcttgacca cctggcagag aagttccggc agaaggcttc catccacgag gcctggactg
     1441 atgggaagga ggccatgctg aagcaacggg actacgagac agccaccctg tcagacatca
     1501 aagctctgat ccgaaaacat gaggcctttg aaagtgacct ggctgcacac caggaccgag
     1561 tggagcagat tgctgcaatt gctcaggagc tcaacgagct ggactactat gactcccaca
     1621 acgtcaacac acggtgccag aagatctgcg accagtggga taacctgggc tctctgacac
     1681 acagtcgcag ggaagccttg gagaaaacag agaaacagct agagaccatc gaccagctac
     1741 atttggagta tgccaagcgg gctgcaccct tcaacaactg gatggagagt gccatggagg
     1801 acctgcagga catgttcatc gtccacacca tcgaggagat cgagggcctg atctcagccc
     1861 atgaccagtt caagtccacc ctgccagatg ctgacaggga gcgtgaggcc atcctggcca
     1921 tccacaagga ggcccagagg atcgctgaga gcaatcacat caagctgtcg ggcagcaatc
     1981 cctacaccac tgttaccccc cagatcatca actccaagtg ggagaaggtg cagcagctgg
     2041 tgccaaagag ggaccatgca ctcctggagg agcagagcaa gcagcaatcc aatgagcacc
     2101 ttcgccgaca gttcgccagc caagccaata tggtggggcc gtggatccag accaagatgg
     2161 aggagatcgg gcgcatctcc attgagatga acgggactct ggaagaccag ctgagccacc
     2221 tgaagcagta cgagcgcagc atcgtggact acaagcccag cctggacctg ctggagcagc
     2281 agcaccagct catccaggag gccctcatct tcgacaacaa gcacaccaac tacacaatgg
     2341 agcatatccg tgtgggctgg gagcagctgc tcacgaccat tgcccgcacc atcaatgagg
     2401 tggaaaacca gatcctcacc cgagatgcca agggtatcag ccaggagcag atgcaggagt
     2461 tccgggcatc cttcaaccat tttgacaagg accatggcgg ggcactgggg cctgaggaat
     2521 tcaaggcctg cctcatcagc ctgggctatg acgtggagaa tgaccggcag ggtgatgctg
     2581 agttcaaccg gatcatgagt gtggttgatc ccaaccatag tggcctcgtg accttccaag
     2641 ccttcattga cttcatgtca agggagacca cagacacaga cacagctgat caggtcatcg
     2701 cctccttcaa ggtcctggca ggagacaaga acttcatcac tgctgaggaa ctgcggagag
     2761 agctgccccc cgaccaggcc gagtactgca tcgcccgcat ggcaccctac caggggcctg
     2821 atgctgctcc tggcgccctt gactacaagt ccttctccac agccctctat ggggagagcg
     2881 acctgtgagg cccttggcca ccctgaacag agactccctt ggcctcccgg aggggctgag
     2941 gagccctgcc cccaccctga ctctgtatct atgcaaagag ctctttgtgg tccttctagg
     3001 gggtcaggca gggaggggct aggcaggcct ctctttctca gtcttgtagg ggagggaggc
     3061 ttggcaatca gcatttctgg tctgctaaat atatacgctg tgctgtgggt tcttagccga
     3121 ttggagggga caacggactc ctgtgcccca gagatacctt cctatcccag gtcccctctg
     3181 cccctgaggc cccttcaaag cctctcacat ctaggccaaa gccccatgtg ccttgtccag
     3241 gaaccgcctg ccctaagaag tccagcagag ggcacccagc tcccctggac cagattccag
     3301 tggctcctcc caggagcaga ggacctgcca tagcccttct ctgcctcagg agtggctgcg
     3361 gggcaatcag ccccagccca cctggcctct gagtctcaga ttttctaagg accaaaacca
     3421 acaaaacaca aagaatggaa cagaaacaaa caaaaaaatc atttaaaaaa aaaaaaagga
     3481 aaaaaaaaac ttaaaaaaaa aaagaaaact ttaaaaactt aagaccttcc agagaattac
     3541 tatttacttt attaacttac ggatttatta tataaatata tatccatcta gctgcatatc
     3601 tctgccacct ctctcactct cataatgaag acatagctga ttcaatgccc tccccagtcc
     3661 ctcctgattt tcccctcgtg gcggtgggca gaggtctctg gggaccttct gaggtggagg
     3721 caggctgctg gcctgcctgc ctggtcgtta atgggtttct ctccactcgc ccccttgagt
     3781 ttattctgat tgatttttat tttcttggtt tctggataaa ccaccctctg gggatgggag
     3841 aataaaacat gtaatatttt taagaagga
//
