LOCUS       NM_007392               2572 bp    mRNA    linear   ROD 04-MAR-2025
DEFINITION  Mus musculus actin alpha 2, smooth muscle, aorta (Acta2), mRNA.
ACCESSION   NM_007392 NM_183274
VERSION     NM_007392.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Liu,S., Li,Y., Huang,D., Liu,M., Zhang,X., Zhao,H., Liu,H., Li,Q.
            and Chen,Z.
  TITLE     Single-Cell RNA-Seq and Histological Analysis Reveals Dynamic Lrig1
            Expression During Salivary Gland Development
  JOURNAL   J Cell Physiol 240 (1), e31487 (2025)
   PUBMED   39587709
REFERENCE   2  (bases 1 to 2572)
  AUTHORS   Takebe,H., Sato,H., Mizoguchi,T. and Hosoya,A.
  TITLE     Localization of alpha-smooth muscle actin in osteoblast
            differentiation during periodontal development
  JOURNAL   Cell Tissue Res 399 (1), 119-127 (2025)
   PUBMED   39579220
REFERENCE   3  (bases 1 to 2572)
  AUTHORS   Defamie,V., Aliar,K., Sarkar,S., Vyas,F., Shetty,R., Reddy
            Narala,S., Fang,H., Saw,S., Tharmapalan,P., Sanchez,O., Knox,J.J.,
            Waterhouse,P.D. and Khokha,R.
  TITLE     Metalloproteinase inhibitors regulate biliary progenitor cells
            through sDLK1 in organoid models of liver injury
  JOURNAL   J Clin Invest 135 (3), e164997 (2024)
   PUBMED   39699962
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2572)
  AUTHORS   Nasim,S., Abujamra,B.A., Chaparro,D., Nogueira,P.D.S., Riva,A.,
            Hutcheson,J.D. and Kos,L.
  TITLE     Multiple cell types including melanocytes contribute to
            elastogenesis in the developing murine aortic valve
  JOURNAL   Sci Rep 14 (1), 25481 (2024)
   PUBMED   39461968
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2572)
  AUTHORS   N'Guetta,P.Y., McLarnon,S.R., Tassou,A., Geron,M., Shirvan,S.,
            Hill,R.Z., Scherrer,G. and O'Brien,L.L.
  TITLE     Comprehensive mapping of sensory and sympathetic innervation of the
            developing kidney
  JOURNAL   Cell Rep 43 (10), 114860 (2024)
   PUBMED   39412983
REFERENCE   6  (bases 1 to 2572)
  AUTHORS   Foster,D.N., Min,B., Foster,L.K., Stoflet,E.S., Sun,S., Getz,M.J.
            and Strauch,A.R.
  TITLE     Positive and negative cis-acting regulatory elements mediate
            expression of the mouse vascular smooth muscle alpha-actin gene
  JOURNAL   J Biol Chem 267 (17), 11995-12003 (1992)
   PUBMED   1601869
REFERENCE   7  (bases 1 to 2572)
  AUTHORS   Harris,D.E., Warshaw,D.M. and Periasamy,M.
  TITLE     Nucleotide sequences of the rabbit alpha-smooth-muscle and
            beta-non-muscle actin mRNAs
  JOURNAL   Gene 112 (2), 265-266 (1992)
   PUBMED   1555776
REFERENCE   8  (bases 1 to 2572)
  AUTHORS   Rhyner,T.A., Lecain,E., Mallet,J. and Pessac,B.
  TITLE     Isolation of cDNAs from a mouse astroglial cell line by a
            subtracted cDNA library
  JOURNAL   J Neurosci Res 27 (2), 144-152 (1990)
   PUBMED   1701491
REFERENCE   9  (bases 1 to 2572)
  AUTHORS   Min,B.H., Foster,D.N. and Strauch,A.R.
  TITLE     The 5'-flanking region of the mouse vascular smooth muscle
            alpha-actin gene contains evolutionarily conserved sequence motifs
            within a functional promoter
  JOURNAL   J Biol Chem 265 (27), 16667-16675 (1990)
   PUBMED   2398068
REFERENCE   10 (bases 1 to 2572)
  AUTHORS   Schiffer,S.P., Prochazka,M., Jezyk,P.F., Roderick,T.H., Yudkoff,M.
            and Patterson,D.F.
  TITLE     Organic aciduria and butyryl CoA dehydrogenase deficiency in
            BALB/cByJ mice
  JOURNAL   Biochem Genet 27 (1-2), 47-58 (1989)
   PUBMED   2712823
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC102285.7 and AK017374.1.
            
            On Jan 4, 2013 this sequence version replaced NM_007392.2.
            
            Summary: The protein encoded by this gene belongs to the actin
            family of proteins, which are highly conserved proteins that play a
            role in cell motility, structure and integrity. Alpha, beta and
            gamma actin isoforms have been identified, with alpha actins being
            a major constituent of the contractile apparatus, while beta and
            gamma actins are involved in the regulation of cell motility. This
            actin is an alpha actin that is found in smooth muscle. [provided
            by RefSeq, Feb 2021].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data because no single transcript from the same
            strain was available for the full length of the gene. The extent of
            this transcript is supported by transcript alignments and
            orthologous data.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK017374.1, AK156331.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-37                AC102285.7         84127-84163         c
            38-1817             AK017374.1         1-1780
            1818-2572           AC102285.7         69126-69880         c
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="19"
                     /map="19 29.41 cM"
     gene            1..2572
                     /gene="Acta2"
                     /gene_synonym="0610041G09Rik; a-SMA; Actvs; alphaSMA;
                     SMAalpha; SMalphaA"
                     /note="actin alpha 2, smooth muscle, aorta"
                     /db_xref="GeneID:11475"
                     /db_xref="MGI:MGI:87909"
     exon            1..84
                     /gene="Acta2"
                     /gene_synonym="0610041G09Rik; a-SMA; Actvs; alphaSMA;
                     SMAalpha; SMalphaA"
                     /inference="alignment:Splign:2.1.0"
     exon            85..236
                     /gene="Acta2"
                     /gene_synonym="0610041G09Rik; a-SMA; Actvs; alphaSMA;
                     SMAalpha; SMalphaA"
                     /inference="alignment:Splign:2.1.0"
     CDS             108..1241
                     /gene="Acta2"
                     /gene_synonym="0610041G09Rik; a-SMA; Actvs; alphaSMA;
                     SMAalpha; SMalphaA"
                     /note="alpha-actin-2; actin, alpha, vascular smooth
                     muscle"
                     /codon_start=1
                     /product="actin, aortic smooth muscle"
                     /protein_id="NP_031418.1"
                     /db_xref="CCDS:CCDS29757.1"
                     /db_xref="GeneID:11475"
                     /db_xref="MGI:MGI:87909"
                     /translation="MCEEEDSTALVCDNGSGLCKAGFAGDDAPRAVFPSIVGRPRHQG
                     VMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHSFYNELRVAPEE
                     HPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDG
                     VTHNVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKL
                     CYVALDFENEMATAASSSSLEKSYELPDGQVITIGNERFRCPETLFQPSFIGMESAGI
                     HETTYNSIMKCDIDIRKDLYANNVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAP
                     PERKYSVWIGGSILASLSTFQQMWISKQEYDEAGPSIVHRKCF"
     exon            237..365
                     /gene="Acta2"
                     /gene_synonym="0610041G09Rik; a-SMA; Actvs; alphaSMA;
                     SMAalpha; SMalphaA"
                     /inference="alignment:Splign:2.1.0"
     exon            366..476
                     /gene="Acta2"
                     /gene_synonym="0610041G09Rik; a-SMA; Actvs; alphaSMA;
                     SMAalpha; SMalphaA"
                     /inference="alignment:Splign:2.1.0"
     exon            477..561
                     /gene="Acta2"
                     /gene_synonym="0610041G09Rik; a-SMA; Actvs; alphaSMA;
                     SMAalpha; SMalphaA"
                     /inference="alignment:Splign:2.1.0"
     exon            562..723
                     /gene="Acta2"
                     /gene_synonym="0610041G09Rik; a-SMA; Actvs; alphaSMA;
                     SMAalpha; SMalphaA"
                     /inference="alignment:Splign:2.1.0"
     exon            724..915
                     /gene="Acta2"
                     /gene_synonym="0610041G09Rik; a-SMA; Actvs; alphaSMA;
                     SMAalpha; SMalphaA"
                     /inference="alignment:Splign:2.1.0"
     exon            916..1097
                     /gene="Acta2"
                     /gene_synonym="0610041G09Rik; a-SMA; Actvs; alphaSMA;
                     SMAalpha; SMalphaA"
                     /inference="alignment:Splign:2.1.0"
     exon            1098..2572
                     /gene="Acta2"
                     /gene_synonym="0610041G09Rik; a-SMA; Actvs; alphaSMA;
                     SMAalpha; SMalphaA"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1360..1365
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Acta2"
                     /gene_synonym="0610041G09Rik; a-SMA; Actvs; alphaSMA;
                     SMAalpha; SMalphaA"
                     /note="hexamer: AATAAA"
     polyA_site      1386
                     /gene="Acta2"
                     /gene_synonym="0610041G09Rik; a-SMA; Actvs; alphaSMA;
                     SMAalpha; SMalphaA"
                     /note="major polyA site"
     polyA_site      1407
                     /gene="Acta2"
                     /gene_synonym="0610041G09Rik; a-SMA; Actvs; alphaSMA;
                     SMAalpha; SMalphaA"
     regulatory      1770..1775
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Acta2"
                     /gene_synonym="0610041G09Rik; a-SMA; Actvs; alphaSMA;
                     SMAalpha; SMalphaA"
                     /note="hexamer: ATTAAA"
     polyA_site      1820
                     /gene="Acta2"
                     /gene_synonym="0610041G09Rik; a-SMA; Actvs; alphaSMA;
                     SMAalpha; SMalphaA"
     polyA_site      1824
                     /gene="Acta2"
                     /gene_synonym="0610041G09Rik; a-SMA; Actvs; alphaSMA;
                     SMAalpha; SMalphaA"
     regulatory      2553..2558
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Acta2"
                     /gene_synonym="0610041G09Rik; a-SMA; Actvs; alphaSMA;
                     SMAalpha; SMalphaA"
                     /note="hexamer: AATAAA"
     polyA_site      2572
                     /gene="Acta2"
                     /gene_synonym="0610041G09Rik; a-SMA; Actvs; alphaSMA;
                     SMAalpha; SMalphaA"
ORIGIN      
        1 caaggggcta tataaccctt cagcgttcag cctcccggga caccacccac ccagagtgga
       61 gaagcccagc cagtcgctgt caggaaccct gagacgctgc tccagctatg tgtgaagagg
      121 aagacagcac agccctggtg tgcgacaatg gctctgggct ctgtaaggcc ggcttcgctg
      181 gtgatgatgc tcccagggct gttttcccat ccatcgtggg acgtcccaga catcagggag
      241 taatggttgg aatgggccaa aaagacagct atgtggggga tgaagcccag agcaagagag
      301 ggatcctgac gctgaagtat ccgatagaac acggcatcat caccaactgg gacgacatgg
      361 aaaagatctg gcaccactct ttctataacg agcttcgtgt ggcccctgaa gagcatccga
      421 cactgctgac agaggcacca ctgaacccta aggccaaccg ggagaaaatg acccagatta
      481 tgtttgagac cttcaatgtc cccgccatgt atgtggctat tcaggctgtg ctgtccctct
      541 atgcctctgg acgtacaact ggtattgtgc tggactctgg agatggtgtg actcacaacg
      601 tgcctatcta tgagggctat gccctgcctc atgccatcat gcgtctggac ttggctggcc
      661 gagatctcac cgactacctc atgaagatcc tgactgagcg tggctattcc ttcgtgacta
      721 ctgccgagcg tgagattgtc cgtgacatca aggagaagct gtgctatgta gctctggact
      781 ttgaaaatga gatggccacg gccgcctcct cttcctccct ggagaagagc tacgaactgc
      841 ctgacgggca ggtgatcacc attggaaacg aacgcttccg ctgcccagag actctcttcc
      901 agccatcttt cattgggatg gagtcagcgg gcatccacga aaccacctat aacagcatca
      961 tgaagtgtga tattgacatc aggaaggatc tctatgctaa caacgtcctg tcagggggta
     1021 ccaccatgta cccaggcatt gctgacagga tgcagaagga gatcacagcc ctcgcaccca
     1081 gcaccatgaa gatcaagatc attgcccctc cagaacgcaa gtactctgtc tggatcggtg
     1141 gctccatcct ggcttcgctg tctaccttcc agcagatgtg gatcagcaaa caggaatacg
     1201 acgaagctgg gccctccatc gtccaccgca aatgcttcta agtcccccct gctctgcctc
     1261 tagcacacaa ctgtgaacgt tttgtggatc agcgcctcca gttcctttcc aaatcattcc
     1321 tgcccaaagc tttgatttgt tactcgtggt ttttttaaaa ataaatcaga catgtgctac
     1381 ccttaacttg tagccctggc ctagcaacac tgattcaatt gtgtttctgt taggtgagaa
     1441 tcatttatta tagggtggcc ccctctagtg gtcaggaata gatgtgacac aactagaaaa
     1501 gtatattccc aggcatggat ggcatcaatc acttcaacag aggaaggtcc acttaaatga
     1561 tgtaaacggg gtatttgaga gcgtatagga cttgtataga ttacatataa taatatatat
     1621 tagtaccaat aggaaaaatc gacactgact tcacagctag agtgactatt ttactttata
     1681 catacaggtg accagatagg taaaaaacaa ttttacgtgt atttaaaatt tggcaagtaa
     1741 tatggtttta ttaaaatcac atcttttgta ttaaagcata cacacgtgca tggatatgca
     1801 tgcgtgactt ttaacttgca tcaagccttg cgtattattt aactttaacg ttcatcaaac
     1861 ttggcatatt taaggaaata attttttagt tagtttttaa aaagcacaca ccttttagaa
     1921 cccaagaagc tatttaaaaa gtctcactat gcacctggat cattgcttcc tcctcctcct
     1981 ttggccaaca tccaggaaaa aatgcagtag atatagttac agtaagagga agggggaaaa
     2041 cggcagtact tccaaggact tgtcatacca tgcatcgtac acaagactct cacacgtcat
     2101 cactactatg tgcagatggt gttatcatca gtcatcagat gaggaagcca agggtgtccc
     2161 caaaaatgca gttacttcca tagacagcta aagacttgaa cagttccttt gcctatcaga
     2221 atgggaacta cagaggttta tttgagtttt tttttttttt ttttttttgg ttgtttggtt
     2281 ggttgggttt ttgtgtgttt tttaatacca gacatattag ggctggagaa ttggatctgt
     2341 ggttaaggat actggctgtt cttgcagaag acctggcctc tactcctagt accctcatgg
     2401 tagcttgcac ctgcttggct gtgactcagg tcccagggga tccagatccc ttctctgacc
     2461 tttgctggca ccaggcatgc atgtggtaca cacatagaca tgcaggtaaa acagtcaagc
     2521 atataaaaat aataaaataa gttaattaaa tgaataaaat gtgacaaccg ga
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