LOCUS       NM_146197               6527 bp    mRNA    linear   ROD 09-FEB-2025
DEFINITION  Mus musculus acyl-CoA synthetase medium-chain family member 2
            (Acsm2), transcript variant 3, mRNA; nuclear gene for mitochondrial
            product.
ACCESSION   NM_146197
VERSION     NM_146197.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 6527)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 6527)
  AUTHORS   Watanabe,H., Paxton,R.L., Tolerico,M.R., Nagalakshmi,V.K.,
            Tanaka,S., Okusa,M.D., Goto,S., Narita,I., Watanabe,S.,
            Sequeira-Lomicronpez,M.L.S. and Gomez,R.A.
  TITLE     Expression of Acsm2, a kidney-specific gene, parallels the function
            and maturation of proximal tubular cells
  JOURNAL   Am J Physiol Renal Physiol 319 (4), F603-F611 (2020)
   PUBMED   32830538
  REMARK    GeneRIF: Expression of Acsm2, a kidney-specific gene, parallels the
            function and maturation of proximal tubular cells.
REFERENCE   3  (bases 1 to 6527)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643.. PMID: 29144450]
REFERENCE   4  (bases 1 to 6527)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   5  (bases 1 to 6527)
  AUTHORS   Calvo,S.E., Pagliarini,D.J. and Mootha,V.K.
  TITLE     Upstream open reading frames cause widespread reduction of protein
            expression and are polymorphic among humans
  JOURNAL   Proc Natl Acad Sci U S A 106 (18), 7507-7512 (2009)
   PUBMED   19372376
  REMARK    GeneRIF: Includes the study of an upstream ORF in this gene, and
            shows that it functions to reduce protein levels by ~50%.
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC125147.4, AK165353.1,
            AC134587.3 and AK165449.1.
            
            On May 5, 2010 this sequence version replaced NM_146197.3.
            
            Transcript Variant: This variant (3) differs in the 5' UTR, lacks a
            portion of the 5' coding region, and uses a downstream start codon,
            compared to variant 1. The encoded isoform (3) is shorter at the
            N-terminus, compared to isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK057650.1, AK165353.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849385 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on conservation,
                                                  expression
            regulatory uORF                    :: PMID: 19372376
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-3                 AC125147.4         172762-172764
            4-2424              AK165353.1         2-2422
            2425-5954           AC134587.3         51381-54910
            5955-6527           AK165449.1         2226-2798
FEATURES             Location/Qualifiers
     source          1..6527
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 63.97 cM"
     gene            1..6527
                     /gene="Acsm2"
                     /note="acyl-CoA synthetase medium-chain family member 2"
                     /db_xref="GeneID:233799"
                     /db_xref="MGI:MGI:2385289"
     exon            1..106
                     /gene="Acsm2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      23..64
                     /regulatory_class="uORF"
                     /gene="Acsm2"
                     /experiment="DESCRIPTION:regulatory uORF[PMID:19372376]"
                     /note="this uORF is predicted to encode a 13 aa peptide
                     (PMID:19372376)"
     exon            107..291
                     /gene="Acsm2"
                     /inference="alignment:Splign:2.1.0"
     CDS             115..1842
                     /gene="Acsm2"
                     /EC_number="6.2.1.2"
                     /EC_number="6.2.1.25"
                     /note="isoform 3 is encoded by transcript variant 3;
                     acyl-coenzyme A synthetase ACSM2, mitochondrial;
                     butyrate--CoA ligase 2; middle-chain acyl-CoA synthetase
                     2; butyryl-coenzyme A synthetase 2; benzoate--CoA ligase"
                     /codon_start=1
                     /product="acyl-coenzyme A synthetase ACSM2, mitochondrial
                     isoform 3"
                     /protein_id="NP_666309.1"
                     /db_xref="CCDS:CCDS21782.1"
                     /db_xref="GeneID:233799"
                     /db_xref="MGI:MGI:2385289"
                     /translation="MHHLWKIPRLFTLWGNEISCRTFHMNIKKLIPIQWGHQEAPAKF
                     NFASDVIDHWASVEKAGKRSSGPALWWMNGSGKEIKWSFRELSEASKQTANVLSGACG
                     LHRGDRVAVVLPRIPEWWLMILGCMRTGLVFMPGTIQMRSSDILYRLQASKARAIVAG
                     DEVAQEVDAVAPDCSFLKIKLLVSENSREGWLNFKALLKEASTIHQCVETESRESAAI
                     YFTSGTSGPPKMAEHSHCSLGIKAKMDAASWTGLSTSDIIWTISDTAWIMNILGAFLE
                     PWVLGACIFVHLLPKFDSQTVLKVLSSYPINTLVGAPIIYRMLLQQDLSSYKFPHLHS
                     CFSGGETLLPETLENWKAKTGLEIREIYGQTETGLICRVSRTMKVKPGYLGTAFAHYD
                     VQVIDEQGNVLPPGKEGDIAIRVKPIWPIGMFSGYVDNPKKTQDNIRGDFWLMGDRGI
                     KDPEGYFHFIGRSDDIINSSGYRIGPSEVENALMEHPAVSETAVISSPDPSRGEVVKA
                     FVVLAPEFLSHDRDQLTKVLQEHVKSVTAPYKYPRKVEFVLDLPKTVTGKIERAKLRA
                     KEWKTSGRA"
     exon            292..502
                     /gene="Acsm2"
                     /inference="alignment:Splign:2.1.0"
     exon            503..710
                     /gene="Acsm2"
                     /inference="alignment:Splign:2.1.0"
     exon            711..857
                     /gene="Acsm2"
                     /inference="alignment:Splign:2.1.0"
     exon            858..1011
                     /gene="Acsm2"
                     /inference="alignment:Splign:2.1.0"
     exon            1012..1091
                     /gene="Acsm2"
                     /inference="alignment:Splign:2.1.0"
     exon            1092..1215
                     /gene="Acsm2"
                     /inference="alignment:Splign:2.1.0"
     exon            1216..1296
                     /gene="Acsm2"
                     /inference="alignment:Splign:2.1.0"
     exon            1297..1398
                     /gene="Acsm2"
                     /inference="alignment:Splign:2.1.0"
     exon            1399..1526
                     /gene="Acsm2"
                     /inference="alignment:Splign:2.1.0"
     exon            1527..1626
                     /gene="Acsm2"
                     /inference="alignment:Splign:2.1.0"
     exon            1627..1746
                     /gene="Acsm2"
                     /inference="alignment:Splign:2.1.0"
     exon            1747..6527
                     /gene="Acsm2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2411..2416
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Acsm2"
                     /note="hexamer: ATTAAA"
     polyA_site      2434
                     /gene="Acsm2"
                     /note="major polyA site"
     regulatory      6497..6502
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Acsm2"
                     /note="hexamer: AATAAA"
     polyA_site      6525
                     /gene="Acsm2"
ORIGIN      
        1 ttcagtgctc ttctctccac tgatgctgca ggaattctca aacgacttcc caagagggaa
       61 atagacaaag ctactgtcag agagagttcc agaagaagac cagaagctct gactatgcat
      121 catctatgga aaattccaag acttttcact ctctggggga atgagatttc ctgccgtact
      181 ttccatatga atatcaagaa actaataccc atacagtggg gccaccagga agcacctgcc
      241 aagttcaact tcgctagtga tgtgatagat cactgggcca gcgtagagaa ggctggcaag
      301 agatcttcag gtccagccct gtggtggatg aatggcagtg ggaaagaaat aaagtggagc
      361 ttccgagaac tgagtgaagc cagtaaacaa acggccaatg ttctctcggg agcctgtggc
      421 ctacatcgtg gggaccgtgt ggcagtagtg cttccccgaa tcccagagtg gtggctgatg
      481 atcctgggct gcatgcggac aggccttgtt ttcatgcctg ggaccatcca gatgagatcc
      541 tcagacatac tctacaggct gcaggcatcc aaggccaggg ccattgtggc tggggacgaa
      601 gtagctcagg aagtggatgc tgtggcccct gactgctctt ttctgaaaat caagttgctg
      661 gtgtctgaaa acagtcgaga aggatggcta aacttcaagg cattgctaaa agaggcatcc
      721 accattcatc agtgtgtgga gacagaaagc cgagaatcag cagccatcta cttcactagt
      781 gggaccagtg gccctcccaa gatggcagaa cactcccact gcagcctggg catcaaggcc
      841 aagatggatg ctgccagctg gacaggcctg agtacttctg acataatatg gaccatctca
      901 gacacggctt ggataatgaa cattttagga gcgtttctgg aaccttgggt attgggagca
      961 tgcatatttg tccatctttt gccaaagttt gattcacaaa ctgttctaaa ggtgctttcc
     1021 agctatccca tcaataccct ggtgggtgcc cccatcattt accggatgtt gctacaacag
     1081 gatctttcca gttacaaatt tccacatctg catagctgct tcagtggagg agagaccctt
     1141 ctcccagaga ctctggagaa ctggaaagcc aagacaggac tggaaatccg agaaatctat
     1201 ggccagacag aaacgggact tatctgcaga gtttccagga caatgaaagt caaaccaggc
     1261 tacctgggaa cagcctttgc tcactatgat gtacaggtca tagatgagca gggcaatgtt
     1321 ctgccccctg gcaaggaagg agacatagct atcagggtga agcccatctg gcctatcggc
     1381 atgttctctg gatatgtgga caatcccaag aagacacagg ataatattcg aggagacttt
     1441 tggcttatgg gagaccgggg aatcaaggat ccagaagggt atttccactt catagggcgg
     1501 tcagacgata tcattaattc cagtgggtac cggattggac cttccgaggt ggaaaatgca
     1561 ctgatggagc atcctgccgt aagcgaaaca gcagtgatca gcagcccaga cccttccaga
     1621 ggagaggtgg tgaaggcatt tgtggtccta gcccctgaat tcctgtccca tgaccgagac
     1681 cagctcacta aggtgcttca ggagcacgtg aagtcagtga cagcacccta caagtacccc
     1741 aggaaggtgg agttcgtctt agacctgccc aagaccgtca caggaaaaat tgagcgagca
     1801 aagcttcgag ccaaggaatg gaaaacatca ggacgagcct aggcccagtg agactcctga
     1861 ggcttttgat ggggttctgt cttccccaat cctttcctgt gacttcttcg tctccactta
     1921 tggagatata agattattca cacagaggca cgtatgtgat tttggtctct gtttggttat
     1981 tgacaaagaa caatgacacg tttgacacta aaagaaaaaa gaaagaggga ataagagatt
     2041 ttcaggggaa aaattgagtc agaaaagtgc taaaacagca agggaaagaa attgtcaatt
     2101 caagtgggca ggaagtggga gaatgaaggg gaaaagagaa tgaggagagg gcaaaaaaag
     2161 aaggaactga agaaaagtag aaaaggagtg tgtggaatgg cactagtgtc ttccccaagt
     2221 tggctcaatc cttagggaca tcatgttcaa aggaggagga aataccacgt tgtcatgccc
     2281 atgtcctaca catgttgtga gccatcttgc ttcatctttg tgtcatttgc aggttcaact
     2341 tctacacccc aaaacatatc acttccttta gctcacagtc gttgtcatat cttcacttcc
     2401 ttgaatcaat attaaaatca ttttaaaatc aaaactccaa aggcattctt tgggcaagac
     2461 aggagaggca tgtgtgcagc agcttatgaa ctgaaaaacc cttagagagc tgatggatga
     2521 tgctgttaat gaaaaaccac gagctcagct accagggcaa cctgatcagc agatctaggt
     2581 gtcaaggagc tgctgagggg gcagagtctc acttgtaacc tagaaagggt taatgaatct
     2641 tcttggtaga actgggcaat caggaaatga aagcaaaggt tctcaagagc cagggtcttc
     2701 ttggaaaaca cccagtgaca ataacagtga caataacagt gacaataaca gaacactact
     2761 taccaaacac ttgccaggag ctagtctgtc tgtctgtctc tctgtctctg tctctgtctc
     2821 tgtctctctg tctctctttc tctctctccc tctctctctc cctctctctc tctctctctc
     2881 tctctctctc tctctgactc cagctactgt cctcagtttt acagaggagg aaaccagaga
     2941 tcagatgata aataatccac cacggtcaca caatagtcaa gtcacagaga caaggtctgt
     3001 ctatataact cccgtgcttt gaacagcttc ccttgcctca tcagcgtgtt tgctttggct
     3061 actacagttt gtatcagtca gaattcttca ggcaaaaaaa aggtctccac ctaacacctc
     3121 tctctccttc cctcccttcc aacctccctt tctccctccc tccttccttc ccttccttta
     3181 aagtatgaaa acccaatact ccagatgttt gtgttaagaa aagctattaa gttttagaga
     3241 gttcatctac aaggtgtgtg tgtgtgtcac attgtgcaca ttttagacag cacaagctcg
     3301 tgcatttact tgagttgcac actggttggc atatcctgaa agaatggtca taagggtggc
     3361 attcaccata gtaataataa taataataat aataataata ataataataa taataacaac
     3421 aacctataaa ttagtcactg aggtttggaa ataaatccca gtctggcagt tccagaacta
     3481 atattttaat taagcctcat tgggtttcaa ggtttataag catattagac ttgaaatata
     3541 ttctatttca agatctgaga catttcgtgg actgtcatcc agaactcatg gggatatagc
     3601 tggcaacagg gaggccaaga atgcaggcag acctccaggc acctccacgg gagcagatga
     3661 tcacatccct ctcccctttt atcccacacc tgagcatcca catttaacct gctccctgtg
     3721 aaaatgtagg atggtgtcat aacataagct ctcctcattt cctaccctct gacactgtta
     3781 ccttccacct tcagggtcac caacttaatt ctaaaaagac aaaatctgat tgactgatgt
     3841 gagttgttta catggagtct gatccacatg taacacaaac atggcatcag gtattccaac
     3901 atggttcact agaagaaaac tgatagaaga aaggggccag cccgtgtgat cgatcagcag
     3961 gtgaaggaac ctgtcgccaa gtctgatgac ctgagttcaa tccccaagac ccatatggtt
     4021 gaaagagaac caactcctgc atgttgtcct ctgacctcca taagtacaca cacacacaca
     4081 cacacacaca cacacacaca cacacacaca cacaataaat aaataaataa ataaataaat
     4141 aaataaataa ataaataaat aaataaaata aagtaattaa aaggttagcc tgatatttgg
     4201 acacaaattg accagctctt gaaaagctca caggtatgtt tggacaactt caaagcctct
     4261 cttctctcta gcatcttttc ttgatgcctt ttctctctgc tcatttttca cactaaagtg
     4321 gccatgctta ggactcatct aatgaaaact ggggggctag agaaaaatct ctactagtca
     4381 aggctctctc ataatagagg gcactggaca gtttcagttg agtgataaca tctgacctaa
     4441 acactagtta tctgtgtccc atactccttt tctgttgctc ctggctagtc attcttaatc
     4501 tttggcctac gatcattatg ctatcgtgag aacgattgcg tgtgtggata tgtggataca
     4561 tgttcatgca tattcaggta catgagtgag tgaaaggtta catgcacatg aacgttgtgt
     4621 atgaacacca aaggaaaacc atgagtgtcg ttctcatgaa cactgtccat ctcctttaag
     4681 aaagaatttc tcatgggcct ggagatcacc agttaggagc agggatcttc ctgtctctgc
     4741 ctccctagca ctgggatgac aagcaatgct cccaccttcc ttttctaagg tgtctctccc
     4801 accttgagaa tgagttttac tatatcccat tactctgggt ccagcctatg agccctgctt
     4861 tcggtgtcgt gcatagcgga agccctttac ctttaagagg aatcaatgct gtccctcagt
     4921 tatgaccctg actcccttca ttctgctcca tccttcctgc ctatggctta gataaaatac
     4981 ccgtagtagc ctgtaccttt acaggaggct ggtatgctgt gactgtgaag aagtcaaagc
     5041 tcctgtcttt ttcagcacat gaggaagctt aaccttgtca tcctgttctt tttggttctc
     5101 tacatataat accctgacaa agacttttca aaagtaagca cacatcaacc agcatgcacc
     5161 ctcaacatgc acagcctatc atgtttgcac actttggcat aaaaaattaa cctcaaacag
     5221 ggccatttaa aacaaccatt tcactatgtt tacaaattct gtgggctgag aatttgaaag
     5281 aatagtagtg gaatacatgc gtgcacacac acacaaacac acacatacaa gctctaatac
     5341 atgtaagacc tttagttgaa gaacttgggt gttgaaagaa tttgatggat aggattaaat
     5401 tgtgcccaag ttcatatgtt gactgtactt gaactggcct tcttatccag gataagtgga
     5461 gttcatgaag gtggatccct catgatagga ttagtggctt tataagaaca gtgattgaga
     5521 aagaattcat tctgttgacg cccatgcaag cggaacagca aactgcttaa gaactcatgg
     5581 aatgtggccc actggttagc accatctcaa tgggtgtgcc ttggagaggc actacatgtc
     5641 ctgaggatca catgctgctg tagagcgtca ctctgcttgt tacccttgtg gtggtcatca
     5701 tgtctccccc taatctatga gctgtatgaa aactctaaga gggtttccag accctcacag
     5761 ggcagtgtca ttggcattta caatagttgt tcttgatctt tctcaagcat tactgctggg
     5821 gcagattaat gattcagtta ccattcagta aagttaggtt aaggtatttt tgctagtggt
     5881 tttgatgtag caaatcttga ggagcaatgt caagtttaga aaggcgttag aaagtttgat
     5941 aaccgagtga aggatttttg aggtcaggga acaagaataa cagcagtctg actagtacta
     6001 gctggtgcat ctctcttgct gggcgggcga tcaaatggtg attcatttct tgtacctatt
     6061 ttcgccctca gcttcctgat atgatttaat aaaaagctgt taatgtgtaa atgtataccc
     6121 tgaagattta gagtagaaat gactgatttt ttatataaaa gtttagattt acgattcttt
     6181 gaagattttg tatgattact ttttaagata aattgaaacc tgccagtaag acaacctggg
     6241 gagaaaattg ccttgttaag ttataacaag ttctttggtt atgaatgcat gtgggttctt
     6301 gggaataatt tgtttttaac ccataatatg taaaacattt gatcatgtga agctattgag
     6361 gagcatatgt atctctcttt tttaaaaatg tattctcatt gtaattattc acttaaagaa
     6421 aaatagaatg taaccacatt gtggttttat gctgcttgaa agaatttcct ggtagatata
     6481 taagctatgg aagaaaaata aaaattttgg agcttgcatc atccacc
//
