LOCUS       NM_133240               1161 bp    mRNA    linear   ROD 20-FEB-2025
DEFINITION  Mus musculus acyl-CoA thioesterase 8 (Acot8), transcript variant 1,
            mRNA.
ACCESSION   NM_133240
VERSION     NM_133240.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1161)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1161)
  AUTHORS   Westin,M.A., Hunt,M.C. and Alexson,S.E.
  TITLE     The identification of a succinyl-CoA thioesterase suggests a novel
            pathway for succinate production in peroxisomes
  JOURNAL   J Biol Chem 280 (46), 38125-38132 (2005)
   PUBMED   16141203
  REMARK    GeneRIF: ACOT4 and ACOT8 are responsible for the termination of
            beta-oxidation of dicarboxylic acids of medium-chain length with
            the concomitant release of the corresponding free acids
REFERENCE   3  (bases 1 to 1161)
  AUTHORS   Hunt,M.C., Yamada,J., Maltais,L.J., Wright,M.W., Podesta,E.J. and
            Alexson,S.E.
  TITLE     A revised nomenclature for mammalian acyl-CoA
            thioesterases/hydrolases
  JOURNAL   J Lipid Res 46 (9), 2029-2032 (2005)
   PUBMED   16103133
REFERENCE   4  (bases 1 to 1161)
  AUTHORS   Kikuchi,M., Hatano,N., Yokota,S., Shimozawa,N., Imanaka,T. and
            Taniguchi,H.
  TITLE     Proteomic analysis of rat liver peroxisome: presence of
            peroxisome-specific isozyme of Lon protease
  JOURNAL   J Biol Chem 279 (1), 421-428 (2004)
   PUBMED   14561759
REFERENCE   5  (bases 1 to 1161)
  AUTHORS   Mootha,V.K., Bunkenborg,J., Olsen,J.V., Hjerrild,M.,
            Wisniewski,J.R., Stahl,E., Bolouri,M.S., Ray,H.N., Sihag,S.,
            Kamal,M., Patterson,N., Lander,E.S. and Mann,M.
  TITLE     Integrated analysis of protein composition, tissue diversity, and
            gene regulation in mouse mitochondria
  JOURNAL   Cell 115 (5), 629-640 (2003)
   PUBMED   14651853
REFERENCE   6  (bases 1 to 1161)
  AUTHORS   Hunt,M.C., Solaas,K., Kase,B.F. and Alexson,S.E.
  TITLE     Characterization of an acyl-coA thioesterase that functions as a
            major regulator of peroxisomal lipid metabolism
  JOURNAL   J Biol Chem 277 (2), 1128-1138 (2002)
   PUBMED   11673457
REFERENCE   7  (bases 1 to 1161)
  AUTHORS   Ferdinandusse,S., Denis,S., Mooijer,P.A., Zhang,Z., Reddy,J.K.,
            Spector,A.A. and Wanders,R.J.
  TITLE     Identification of the peroxisomal beta-oxidation enzymes involved
            in the biosynthesis of docosahexaenoic acid
  JOURNAL   J Lipid Res 42 (12), 1987-1995 (2001)
   PUBMED   11734571
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BB604985.1, AK151924.1 and AI122325.1.
            
            On Jul 23, 2009 this sequence version replaced NM_133240.1.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF441166.1, AK151924.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164133, SAMN01164134
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-23                BB604985.1         3-25
            24-1142             AK151924.1         2-1120
            1143-1161           AI122325.1         360-378
FEATURES             Location/Qualifiers
     source          1..1161
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 85.27 cM"
     gene            1..1161
                     /gene="Acot8"
                     /gene_synonym="PTE-1; PTE-2; Pte1"
                     /note="acyl-CoA thioesterase 8"
                     /db_xref="GeneID:170789"
                     /db_xref="MGI:MGI:2158201"
     exon            1..192
                     /gene="Acot8"
                     /gene_synonym="PTE-1; PTE-2; Pte1"
                     /inference="alignment:Splign:2.1.0"
     CDS             65..1027
                     /gene="Acot8"
                     /gene_synonym="PTE-1; PTE-2; Pte1"
                     /EC_number="3.1.2.2"
                     /EC_number="3.1.2.1"
                     /EC_number="3.1.2.27"
                     /EC_number="3.1.2.3"
                     /EC_number="3.1.2.11"
                     /EC_number="3.1.2.5"
                     /note="isoform 1 is encoded by transcript variant 1;
                     acyl-coenzyme A thioesterase 8; choloyl-coenzyme A
                     thioesterase; peroxisomal acyl-CoA thioesterase 2;
                     peroxisomal long-chain acyl-CoA thioesterase 1;
                     peroxisomal acyl-coenzyme A thioester hydrolase 1;
                     peroxisomal acyl-CoA thioesterase 1"
                     /codon_start=1
                     /product="acyl-coenzyme A thioesterase 8 isoform 1"
                     /protein_id="NP_573503.2"
                     /db_xref="CCDS:CCDS17057.1"
                     /db_xref="GeneID:170789"
                     /db_xref="MGI:MGI:2158201"
                     /translation="MSAPEGLGDAHGDADRGDLSGDLRSVLVTSVLNLEPLDEDLYRG
                     RHYWVPTSQRLFGGQIMGQALVAAAKSVSEDVHVHSLHCYFVRAGDPKVPVLYHVERI
                     RTGASFSVRAVKAVQHGKAIFICQASFQQMQPSPLQHQFSMPSVPPPEDLLDHEALID
                     QYLRDPNLHKKYRVGLNRVAAQEVPIEIKVVNPPTLTQLQALEPKQMFWVRARGYIGE
                     GDIKMHCCVAAYISDYAFLGTALLPHQSKYKVNFMASLDHSMWFHAPFRADHWMLYEC
                     ESPWAGGSRGLVHGRLWRRDGVLAVTCAQEGVIRLKPQVSESKL"
     exon            193..329
                     /gene="Acot8"
                     /gene_synonym="PTE-1; PTE-2; Pte1"
                     /inference="alignment:Splign:2.1.0"
     exon            330..555
                     /gene="Acot8"
                     /gene_synonym="PTE-1; PTE-2; Pte1"
                     /inference="alignment:Splign:2.1.0"
     exon            556..713
                     /gene="Acot8"
                     /gene_synonym="PTE-1; PTE-2; Pte1"
                     /inference="alignment:Splign:2.1.0"
     exon            714..908
                     /gene="Acot8"
                     /gene_synonym="PTE-1; PTE-2; Pte1"
                     /inference="alignment:Splign:2.1.0"
     exon            909..1151
                     /gene="Acot8"
                     /gene_synonym="PTE-1; PTE-2; Pte1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1128..1133
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Acot8"
                     /gene_synonym="PTE-1; PTE-2; Pte1"
                     /note="hexamer: AATAAA"
     polyA_site      1151
                     /gene="Acot8"
                     /gene_synonym="PTE-1; PTE-2; Pte1"
                     /note="major polyA site"
ORIGIN      
        1 ttccggaagc gctgggagcc tatataagtt tccgcgtttc tctcaaccgc agccagcagt
       61 gaaaatgtca gcgccagagg gtctgggaga tgctcatggc gacgccgacc gcggcgacct
      121 ttccggggac ctccgtagtg tgctggtcac gagcgtgctc aacctcgagc cgctagatga
      181 agatctctac agaggaaggc attactgggt acctacctcc cagcggctct ttgggggtca
      241 aattatgggc caggccctgg tggctgcagc caagtctgtg agtgaagacg tccatgtcca
      301 ctccctgcac tgctactttg tccgggcagg ggacccgaaa gtgccagtgc tgtaccacgt
      361 agagaggata cggacaggag ccagcttctc agtgcgcgcc gtgaaggctg tgcagcatgg
      421 caaggccatc ttcatctgcc aggcctcctt ccagcagatg cagcccagcc cgctgcagca
      481 ccagttctcc atgccctccg tgcccccgcc agaagacctg ctggatcacg aggccctcat
      541 tgaccagtac ttaagggacc ctaaccttca caagaagtat cgagtggggc tgaaccgagt
      601 tgctgcccag gaggtaccta ttgagatcaa ggtggtgaac ccacccaccc tgacccagct
      661 gcaggcactg gagcccaaac agatgttctg ggtgcgtgcc cggggctaca ttggggaagg
      721 tgacatcaag atgcattgct gtgtggctgc ttatatctct gactacgcct tcctgggtac
      781 agcactgctg ccccaccagt ccaagtataa ggtgaacttc atggcgtcac tggatcactc
      841 catgtggttt catgccccat tccgagccga ccactggatg ctgtacgagt gtgagagccc
      901 ctgggctggt ggctctcgag ggctggtgca tgggcggctg tggcgtcggg atggggtcct
      961 tgctgtgacc tgtgcccagg agggtgtgat ccgattgaag cctcaggtgt cagagagtaa
     1021 gctatagtga aagatgcctg gctcggcctg gggctaaaag gactttgcac tgccatttct
     1081 gaggcaggag tcacagttga gagctgggct tcctgcctct tacatacaat aaagagattg
     1141 ctaacactgg aaaaaaaaaa a
//
