LOCUS       NM_134247               2325 bp    mRNA    linear   ROD 09-FEB-2025
DEFINITION  Mus musculus acyl-CoA thioesterase 4 (Acot4), mRNA.
ACCESSION   NM_134247
VERSION     NM_134247.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2325)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643.. PMID: 29144450]
REFERENCE   3  (bases 1 to 2325)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   4  (bases 1 to 2325)
  AUTHORS   Boj,S.F., van Es,J.H., Huch,M., Li,V.S., Jose,A., Hatzis,P.,
            Mokry,M., Haegebarth,A., van den Born,M., Chambon,P., Voshol,P.,
            Dor,Y., Cuppen,E., Fillat,C. and Clevers,H.
  TITLE     Diabetes risk gene and Wnt effector Tcf7l2/TCF4 controls hepatic
            response to perinatal and adult metabolic demand
  JOURNAL   Cell 151 (7), 1595-1607 (2012)
   PUBMED   23260145
REFERENCE   5  (bases 1 to 2325)
  AUTHORS   Hunt,M.C., Rautanen,A., Westin,M.A., Svensson,L.T. and Alexson,S.E.
  TITLE     Analysis of the mouse and human acyl-CoA thioesterase (ACOT) gene
            clusters shows that convergent, functional evolution results in a
            reduced number of human peroxisomal ACOTs
  JOURNAL   FASEB J 20 (11), 1855-1864 (2006)
   PUBMED   16940157
REFERENCE   6  (bases 1 to 2325)
  AUTHORS   Westin,M.A., Hunt,M.C. and Alexson,S.E.
  TITLE     The identification of a succinyl-CoA thioesterase suggests a novel
            pathway for succinate production in peroxisomes
  JOURNAL   J Biol Chem 280 (46), 38125-38132 (2005)
   PUBMED   16141203
  REMARK    GeneRIF: ACOT4 and ACOT8 are responsible for the termination of
            beta-oxidation of dicarboxylic acids of medium-chain length with
            the concomitant release of the corresponding free acids
REFERENCE   7  (bases 1 to 2325)
  AUTHORS   Westin,M.A., Alexson,S.E. and Hunt,M.C.
  TITLE     Molecular cloning and characterization of two mouse peroxisome
            proliferator-activated receptor alpha (PPARalpha)-regulated
            peroxisomal acyl-CoA thioesterases
  JOURNAL   J Biol Chem 279 (21), 21841-21848 (2004)
   PUBMED   15007068
REFERENCE   8  (bases 1 to 2325)
  AUTHORS   Kikuchi,M., Hatano,N., Yokota,S., Shimozawa,N., Imanaka,T. and
            Taniguchi,H.
  TITLE     Proteomic analysis of rat liver peroxisome: presence of
            peroxisome-specific isozyme of Lon protease
  JOURNAL   J Biol Chem 279 (1), 421-428 (2004)
   PUBMED   14561759
REFERENCE   9  (bases 1 to 2325)
  AUTHORS   Hunt,M.C., Nousiainen,S.E., Huttunen,M.K., Orii,K.E., Svensson,L.T.
            and Alexson,S.E.
  TITLE     Peroxisome proliferator-induced long chain acyl-CoA thioesterases
            comprise a highly conserved novel multi-gene family involved in
            lipid metabolism
  JOURNAL   J Biol Chem 274 (48), 34317-34326 (1999)
   PUBMED   10567408
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK046630.1, BC113185.1 and AC133183.3.
            
            On Nov 20, 2009 this sequence version replaced NM_134247.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK046630.1, AK050420.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849376
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-390               AK046630.1         1-390
            391-1819            BC113185.1         335-1763
            1820-2161           AK046630.1         1820-2161
            2162-2162           AC133183.3         20296-20296         c
            2163-2325           AK046630.1         2163-2325
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="12"
                     /map="12 39.01 cM"
     gene            1..2325
                     /gene="Acot4"
                     /gene_synonym="B430212I04Rik; PTE-Ib; Pte1b; Pte2b"
                     /note="acyl-CoA thioesterase 4"
                     /db_xref="GeneID:171282"
                     /db_xref="MGI:MGI:2159621"
     exon            1..589
                     /gene="Acot4"
                     /gene_synonym="B430212I04Rik; PTE-Ib; Pte1b; Pte2b"
                     /inference="alignment:Splign:2.1.0"
     CDS             133..1398
                     /gene="Acot4"
                     /gene_synonym="B430212I04Rik; PTE-Ib; Pte1b; Pte2b"
                     /EC_number="3.1.2.3"
                     /note="peroxisomal acyl-CoA thioesterase 2B; acyl-coenzyme
                     A thioesterase 4; PTE-2b; peroxisomal long-chain acyl-CoA
                     thioesterase Ib; peroxisomal acyl coenzyme A thioester
                     hydrolase Ib"
                     /codon_start=1
                     /product="peroxisomal succinyl-coenzyme A thioesterase"
                     /protein_id="NP_599008.3"
                     /db_xref="CCDS:CCDS26035.1"
                     /db_xref="GeneID:171282"
                     /db_xref="MGI:MGI:2159621"
                     /translation="MAATLSVEPTGRSCWDEPLSIAVRGLAPEQPVTLRSVLRDEKGA
                     LFRAHARYRADSHGELDLARVPALGGSFSGLEPMGLLWAMEPDRPFWRLIKRDVQTPF
                     LVELEVLDGHEPDGGRRLARTVHERHFMAPGVRRVPVREGRVRATLFLPPGQGPFPGI
                     IDVYGVGGGLLEYRAGLVAGHGFATLALAFYDFEDLPKELNVIEVDYFEEAVRYMLRH
                     PKVKGPDIGLLGLSLGADVCLIMASFLNNVSATVSINGSAFSGNRHIKYKQTMIPPLG
                     HDLRRMKVAFSGILDIVDIRNDAVGGCENPSMIPIEKAKGPILFVAGQDDHCWRSELY
                     TQIASDRLQAHGKERPQVLSYPGTGHYIEPPYFPMCPASLHKIVNEAVIWGGEVKAHS
                     KAQIDAWKQILFFFGKHLGSTHSRASCRL"
     exon            590..792
                     /gene="Acot4"
                     /gene_synonym="B430212I04Rik; PTE-Ib; Pte1b; Pte2b"
                     /inference="alignment:Splign:2.1.0"
     exon            793..2325
                     /gene="Acot4"
                     /gene_synonym="B430212I04Rik; PTE-Ib; Pte1b; Pte2b"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1634..1639
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Acot4"
                     /gene_synonym="B430212I04Rik; PTE-Ib; Pte1b; Pte2b"
                     /note="hexamer: ATTAAA"
     polyA_site      1665
                     /gene="Acot4"
                     /gene_synonym="B430212I04Rik; PTE-Ib; Pte1b; Pte2b"
                     /note="major polyA site"
     regulatory      2113..2118
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Acot4"
                     /gene_synonym="B430212I04Rik; PTE-Ib; Pte1b; Pte2b"
                     /note="hexamer: ATTAAA"
     polyA_site      2135
                     /gene="Acot4"
                     /gene_synonym="B430212I04Rik; PTE-Ib; Pte1b; Pte2b"
ORIGIN      
        1 gtctgtgccc aaattgctga cagatcatgg ttcctctctt gtctgggtcc tagagacgca
       61 ctaggtcggc tccacagcag gccaggctcc gctccggact cgcgtggcgc tccccgaggc
      121 ctccagctcc taatggcagc gacactgagc gtggagccca cgggccgcag ctgctgggac
      181 gagccgctga gcatcgccgt gcgcggcctg gcccccgagc agcccgtcac gctgcgctct
      241 gtcctgcgcg acgagaaggg cgcgctcttc cgagcccacg cgcgctaccg cgcggactcc
      301 cacggcgagc tggacctggc gcgcgtgccc gcgctgggcg gcagcttctc ggggctcgag
      361 cccatggggc tgctctgggc catggagccc gatcggcctt tctggcgcct gatcaagcgc
      421 gacgtgcaga cgcccttctt ggtggagctg gaggtgctgg acggacacga gcccgatggc
      481 gggcggcggc tggcgcggac ggtgcacgag cgtcacttca tggctcccgg ggtgcggcgc
      541 gtgcccgtgc gcgagggccg ggtgcgcgcc acgctcttcc tgcccccagg acaaggaccc
      601 tttccaggca tcatcgatgt ttatggtgtt ggaggaggcc tgttggaata ccgagccggc
      661 cttgtggctg gccatggctt tgccacattg gctctggctt tttatgactt tgaggatctc
      721 cccaaagagc tcaacgtcat agaagtggac tactttgaag aagcagtgcg gtacatgctt
      781 cgacatccaa aggtaaaagg cccagacatt gggcttctgg gtctttccct aggagctgat
      841 gtctgtctga tcatggcttc cttcttgaat aatgtctcag ccacagtttc catcaatggc
      901 tctgcattca gtggaaacag acatatcaag tacaagcaga ccatgattcc gccgttgggc
      961 catgacctga ggagaatgaa ggtggctttc tctggcattc tggacattgt ggatatacgg
     1021 aatgatgctg taggaggctg tgagaacccc agcatgatac caatagagaa ggccaagggg
     1081 cccatcctct ttgtggctgg ccaggatgat cattgctgga ggagtgagtt atatactcag
     1141 atagcctcgg accggttaca ggctcatgga aaggaaaggc cccaggtact ctcttaccct
     1201 gggactgggc attacatcga accaccttac tttcccatgt gcccagcttc cctgcacaaa
     1261 atagtgaacg aagctgtgat ttggggaggg gaggtcaagg ctcactccaa agcccagata
     1321 gatgcatgga agcaaattct attcttcttt ggcaaacacc tgggcagtac ccacagcaga
     1381 gcctcctgta gactgtagtg ggtttgcctg taacagacat ggtagattca aggtcaaatt
     1441 ttaatgatca gtaactatgt gaatcagttt tctgctggat gacatgaaat tcatgtttag
     1501 ggcattaatg gcttataagt cactgtactg aataagttat taatcaatat ggttattcta
     1561 ctaccagcct gtgcttgtta cagattcggc tatatgtgat acaatgagta aagtttaaaa
     1621 acagtgacct cttattaaaa ggtatgattt cttctaagtt cagcatttca gtggtagttg
     1681 cagctgtttt tgttccattt gttgttttgt tttgttgctt ttggggacag gacctcagtt
     1741 ctggccatcc tggaacttgc catgtaaagg aagctggcct tgaactcaca gggattgtca
     1801 tgcttttgcc tcctgcgtga taggattaaa ggctcggccc tacctagtta atactttaac
     1861 tcctggctca gctttaaaga cagtggctgc agctgctgct gcctcatttc atgtcaactt
     1921 gacagaagct ggagtcatgt gagaggaggg agcctgatgt gtgtgcacac acacctgaat
     1981 atacatgtgt gtaccttaca cacacacaaa acaacaacca aaaaatgata attttgccat
     2041 ttcaatggag tagatccagc ctttaaataa tttttcaaat aacatttctg aaaatgcttc
     2101 tcacctgtgt tgattaaaca gcaaagaaac aaaaagttgt gttttctatt caaggtcact
     2161 tggagcctta ttattttgtc ccatttaagc tacaaggaaa tgttaagagt gactccctaa
     2221 aattgagact aaaaaggcaa taaacaaagg aaaagaaact tttagaacta aattaatatg
     2281 gagattgttt taaattaaaa attggtccac agccttcaaa acatt
//
