LOCUS       NM_001346701            2689 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus acyl-CoA thioesterase 3 (Acot3), transcript variant 2,
            mRNA.
ACCESSION   NM_001346701 XM_006515509
VERSION     NM_001346701.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2689)
  AUTHORS   Hunt,M.C., Rautanen,A., Westin,M.A., Svensson,L.T. and Alexson,S.E.
  TITLE     Analysis of the mouse and human acyl-CoA thioesterase (ACOT) gene
            clusters shows that convergent, functional evolution results in a
            reduced number of human peroxisomal ACOTs
  JOURNAL   FASEB J 20 (11), 1855-1864 (2006)
   PUBMED   16940157
REFERENCE   3  (bases 1 to 2689)
  AUTHORS   Westin,M.A., Alexson,S.E. and Hunt,M.C.
  TITLE     Molecular cloning and characterization of two mouse peroxisome
            proliferator-activated receptor alpha (PPARalpha)-regulated
            peroxisomal acyl-CoA thioesterases
  JOURNAL   J Biol Chem 279 (21), 21841-21848 (2004)
   PUBMED   15007068
REFERENCE   4  (bases 1 to 2689)
  AUTHORS   Hunt,M.C., Nousiainen,S.E., Huttunen,M.K., Orii,K.E., Svensson,L.T.
            and Alexson,S.E.
  TITLE     Peroxisome proliferator-induced long chain acyl-CoA thioesterases
            comprise a highly conserved novel multi-gene family involved in
            lipid metabolism
  JOURNAL   J Biol Chem 274 (48), 34317-34326 (1999)
   PUBMED   10567408
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC133183.3.
            
            On Oct 26, 2016 this sequence version replaced XM_006515509.3.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY563098.1, AW044864.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849385, SAMN00849386
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-37                AC133183.3         12676-12712         c
            38-506              AC133183.3         10915-11383         c
            507-709             AC133183.3         7745-7947           c
            710-2689            AC133183.3         4456-6435           c
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="12"
                     /map="12 39.02 cM"
     gene            1..2689
                     /gene="Acot3"
                     /gene_synonym="PTE-Ia; Pte2a"
                     /note="acyl-CoA thioesterase 3"
                     /db_xref="GeneID:171281"
                     /db_xref="MGI:MGI:2159619"
     exon            1..37
                     /gene="Acot3"
                     /gene_synonym="PTE-Ia; Pte2a"
                     /inference="alignment:Splign:2.1.0"
     exon            38..506
                     /gene="Acot3"
                     /gene_synonym="PTE-Ia; Pte2a"
                     /inference="alignment:Splign:2.1.0"
     CDS             50..1315
                     /gene="Acot3"
                     /gene_synonym="PTE-Ia; Pte2a"
                     /EC_number="3.1.2.2"
                     /note="isoform 2 is encoded by transcript variant 2;
                     peroxisomal acyl-CoA thioesterase 2A; acyl-coenzyme A
                     thioesterase 3; peroxisomal long-chain acyl-CoA
                     thioesterase 2; peroxisomal acyl-coenzyme A thioester
                     hydrolase 2a"
                     /codon_start=1
                     /product="acyl-coenzyme A thioesterase 3 isoform 2"
                     /protein_id="NP_001333630.1"
                     /db_xref="CCDS:CCDS83985.1"
                     /db_xref="GeneID:171281"
                     /db_xref="MGI:MGI:2159619"
                     /translation="MAPTVILEPAGGCLCDQPVHIAVRGLAPEQPVTLRSVLRDEKGA
                     LFRAHARYRADSHGELDLARTPALGGSFSGLEPMGLLWAMEPDRPFWRLIKRDVQTPF
                     VVELEVLDGHEPDGGQRLARAVHERHFMAPGVRRVPVREGRVRATLFLPPGTGPFPGI
                     IDLFGIGSGLLEYRASLLAGKGFAVMALAYNNYEDLPKDMDIIHLEYFEEAVTYLLSH
                     PQVTGSGVGVLGISKGGELGFAMASFLKNITAAVIINGSISNIGGNLQYKDETVPSVG
                     INTKRVKRTKDGLKDIVDLLNNPLEGPDQKSLIPVERSDTAFLFLVGQDDHNWKSEFY
                     AREASKRLQAHGKEKPQIICYPETGHHIEPPYFPLCKASLNSLVGGPVIWGGEPRAHA
                     MAQVDAWQQLQTFFHNHLDGKKKTIPAKL"
     exon            507..709
                     /gene="Acot3"
                     /gene_synonym="PTE-Ia; Pte2a"
                     /inference="alignment:Splign:2.1.0"
     exon            710..2689
                     /gene="Acot3"
                     /gene_synonym="PTE-Ia; Pte2a"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1832..1837
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Acot3"
                     /gene_synonym="PTE-Ia; Pte2a"
                     /note="hexamer: AATACA"
     polyA_site      1854
                     /gene="Acot3"
                     /gene_synonym="PTE-Ia; Pte2a"
                     /note="major polyA site"
     regulatory      2667..2672
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Acot3"
                     /gene_synonym="PTE-Ia; Pte2a"
                     /note="hexamer: AATAAA"
     polyA_site      2689
                     /gene="Acot3"
                     /gene_synonym="PTE-Ia; Pte2a"
ORIGIN      
        1 tctgccgtta ttagctcttg accttgtctg tctgctgaac ccgaaccgga tggcacctac
       61 tgtgatcctg gagcccgcgg gtggttgcct ttgtgaccag ccggtgcaca tcgctgtgcg
      121 cggcctggcc ccggagcagc ccgtcacgct gcgctctgtc ctgcgcgacg agaagggcgc
      181 gctcttccga gcccacgcgc gctaccgcgc ggactcccac ggcgagctgg acctggcgcg
      241 cacgcccgcg ctgggcggca gcttctcggg gctcgagccc atggggctgc tctgggccat
      301 ggagcccgat cggcctttct ggcgcctgat caagcgcgac gtgcagacgc cctttgtggt
      361 ggagctggag gtgctggacg gacacgagcc cgacggcggg cagcggctgg cgcgggcggt
      421 gcacgagcgt cacttcatgg cccccggggt tcggcgcgtg ccggtgcgcg agggccgggt
      481 gcgcgccacg ctcttcctgc ccccaggaac tggacccttt cctggaatca tagacctttt
      541 tggaattgga agtggccttc tggagtatcg ggcgagtctg ctggctggga agggctttgc
      601 cgtcatggct ctggcttata acaactacga ggacctccct aaggacatgg acattatcca
      661 cctggagtac tttgaggaag ctgtgaccta cctgctcagt caccctcagg taacaggttc
      721 aggagtcggg gtccttggca tttccaaagg gggtgaactt ggctttgcca tggcctcctt
      781 cttgaaaaat atcacagccg ctgtcatcat caatggctct atatccaaca tcggcggaaa
      841 cttacagtac aaggatgaga ctgtgccctc cgtgggcata aacaccaagc gagtcaagag
      901 aaccaaagat ggcctcaaag acattgtgga tcttctaaac aatcctttgg aaggacctga
      961 ccagaagagc ttgattcccg tggaaaggtc tgacacagcc ttcctgttcc tcgtaggtca
     1021 ggacgaccac aactggaaga gcgagttcta tgccagagag gcctccaaac gcctgcaggc
     1081 gcacgggaag gagaagcccc agatcatctg ctacccagag acaggccacc atattgagcc
     1141 tccttacttc ccactgtgta aggcttccct gaacagcctg gtgggtggtc ctgtcatctg
     1201 gggaggggag cccagggctc atgccatggc ccaggtggac gcttggcagc aactccagac
     1261 ttttttccac aatcatttgg atggcaaaaa gaagacaatc ccagcaaaac tgtgattttt
     1321 atttaagatc tctgatgctt ctctctcctg aggaagcttc cactgtgtgt gtgtttctct
     1381 tttacatcaa tgtagagtct ttccctcctc caactttttt ttcagctgca ggccttattg
     1441 ataagtcttg aaggggagtg attatacaga aaacataagg cattgcagaa actgccgttt
     1501 ttcggaaact cagtctccca gcgagctgta gtgctgaaag aaagaccaag tcactgctac
     1561 atccctggag ttcaaggact acttgctaaa ttgctgatta aaaacaaaca aatgattcca
     1621 tgctgtttag acaaacatcg acaggaactg aaaagatggc tcagcagtta agggcacaca
     1681 ctgctcctat aggacccaag tttgggtcca ggtacgagaa caactgactg taactccagc
     1741 tctaagggat gcagagctct cgtctgacct ccttgggcac ccatacacat gtgtacacac
     1801 acacatatac acacaagcac acatgaataa aaatacatgg tttgaaagag taaacatctg
     1861 tgtggtggtg acacacacct ttcatcccag cactggggct gggagtggga ggatgggagt
     1921 ggggagtggg gagtggggac agaggcagtc agatctctgc aagttggaag ccaacctggt
     1981 ctacagaggg agttccagga catcgagggt tacacagaga gcaaagaaac cctacctgaa
     2041 atacaaacaa agtcataaac aatggtcctg tgtgggctct gtcctgtgga ctccactgac
     2101 tggagattta tgtatacaga ctttcacatg ctacagtcta cacagcacac cacactcact
     2161 cggagggcat ttatgctgta gttcacattt taagcagtct gcaagtgact taaaggttat
     2221 acgtgtatta tatggaaata ctattgtatc cgagaaatgt cagccattgc agatttgcca
     2281 tctgtatcaa atgcttacaa ctaccagtgg acaactctaa catcaaatgt gctgtgacct
     2341 cacaggtgag tttgttcttt tgggatggaa ttactacctt agtcaccaac tagtaagagc
     2401 ccaaaaatat caaatttcaa aggaacttca gtttacatct tgtttttttc ttttgagtaa
     2461 ataaggattt tttttttctg agccaacaaa ttgtgttagg ttcctgttta tgcgtaggga
     2521 tacaagattc tgttgcttca aaccttgata aaatgttatt taactatgca agtaatttta
     2581 acagtggtaa gcatgtatat aataatatca ctaggtacac atagttccaa aataataagc
     2641 atttcttaag ccttagatat gcttaaaata aaaatacttc gatggttaa
//
