LOCUS       NM_028790               3058 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus acyl-CoA thioesterase 12 (Acot12), transcript variant
            1, mRNA.
ACCESSION   NM_028790
VERSION     NM_028790.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3058)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3058)
  AUTHORS   Song,J., Kim,E.H., Yang,J.H., Kim,D., Robby,A.I., Kim,S.A.,
            Park,S.Y., Ryu,J.H. and Jin,E.J.
  TITLE     Upregulated FOXM1 stimulates chondrocyte senescence in
            Acot12-/-Nudt7-/- double knockout mice
  JOURNAL   Theranostics 13 (15), 5207-5222 (2023)
   PUBMED   37908734
  REMARK    GeneRIF: Upregulated FOXM1 stimulates chondrocyte senescence in
            Acot12[-/-]Nudt7[-/-] double knockout mice.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3058)
  AUTHORS   He,H., Sugiyama,A., Snyder,N.W., Teneche,M.G., Liu,X.,
            Maner-Smith,K.M., Goessling,W., Hagen,S.J., Ortlund,E.A.,
            Najafi-Shoushtari,S.H., Acuna,M. and Cohen,D.E.
  TITLE     Acyl-CoA thioesterase 12 suppresses YAP-mediated
            hepatocarcinogenesis by limiting glycerolipid biosynthesis
  JOURNAL   Cancer Lett 565, 216210 (2023)
   PUBMED   37150501
  REMARK    GeneRIF: Acyl-CoA thioesterase 12 suppresses YAP-mediated
            hepatocarcinogenesis by limiting glycerolipid biosynthesis.
REFERENCE   4  (bases 1 to 3058)
  AUTHORS   Park,S., Baek,I.J., Ryu,J.H., Chun,C.H. and Jin,E.J.
  TITLE     PPARalpha-ACOT12 axis is responsible for maintaining cartilage
            homeostasis through modulating de novo lipogenesis
  JOURNAL   Nat Commun 13 (1), 3 (2022)
   PUBMED   34987154
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3058)
  AUTHORS   Park,S., Song,J., Baek,I.J., Jang,K.Y., Han,C.Y., Jun,D.W.,
            Kim,P.K., Raught,B. and Jin,E.J.
  TITLE     Loss of Acot12 contributes to NAFLD independent of lipolysis of
            adipose tissue
  JOURNAL   Exp Mol Med 53 (7), 1159-1169 (2021)
   PUBMED   34285335
  REMARK    GeneRIF: Loss of Acot12 contributes to NAFLD independent of
            lipolysis of adipose tissue.
REFERENCE   6  (bases 1 to 3058)
  AUTHORS   Hunt,M.C., Yamada,J., Maltais,L.J., Wright,M.W., Podesta,E.J. and
            Alexson,S.E.
  TITLE     A revised nomenclature for mammalian acyl-CoA
            thioesterases/hydrolases
  JOURNAL   J Lipid Res 46 (9), 2029-2032 (2005)
   PUBMED   16103133
REFERENCE   7  (bases 1 to 3058)
  AUTHORS   Suematsu,N., Okamoto,K. and Isohashi,F.
  TITLE     Mouse cytosolic acetyl-CoA hydrolase, a novel candidate for a key
            enzyme involved in fat metabolism: cDNA cloning, sequencing and
            functional expression
  JOURNAL   Acta Biochim Pol 49 (4), 937-945 (2002)
   PUBMED   12545200
  REMARK    GeneRIF: possibly involved in fat metabolism.  Here it is sequenced
            and expressed functionally.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC154413.3.
            
            On Mar 22, 2023 this sequence version replaced NM_028790.3.
            
            Sequence Note:.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC025852.1, AK034622.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849377, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-145               AC154413.3         67000-67144         c
            146-215             AC154413.3         58403-58472         c
            216-276             AC154413.3         51077-51137         c
            277-378             AC154413.3         48597-48698         c
            379-514             AC154413.3         45604-45739         c
            515-671             AC154413.3         38415-38571         c
            672-791             AC154413.3         37697-37816         c
            792-942             AC154413.3         36786-36936         c
            943-998             AC154413.3         36180-36235         c
            999-1062            AC154413.3         35837-35900         c
            1063-1146           AC154413.3         33744-33827         c
            1147-1280           AC154413.3         27340-27473         c
            1281-1409           AC154413.3         25632-25760         c
            1410-1536           AC154413.3         24253-24379         c
            1537-3058           AC154413.3         22508-24029         c
FEATURES             Location/Qualifiers
     source          1..3058
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="13"
                     /map="13 47.43 cM"
     gene            1..3058
                     /gene="Acot12"
                     /gene_synonym="1300004O04Rik; 4930449F15Rik; Cach; CACH-1;
                     mCACH-1"
                     /note="acyl-CoA thioesterase 12"
                     /db_xref="GeneID:74156"
                     /db_xref="MGI:MGI:1921406"
     exon            1..145
                     /gene="Acot12"
                     /gene_synonym="1300004O04Rik; 4930449F15Rik; Cach; CACH-1;
                     mCACH-1"
                     /inference="alignment:Splign:2.1.0"
     CDS             16..1686
                     /gene="Acot12"
                     /gene_synonym="1300004O04Rik; 4930449F15Rik; Cach; CACH-1;
                     mCACH-1"
                     /EC_number="3.1.2.1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     cytosolic acetyl-CoA hydrolase; acyl-coenzyme A
                     thioesterase 12; acyl-CoA thioester hydrolase 12;
                     cytoplasmic acetyl-CoA hydrolase 1; acetyl-coenzyme A
                     thioesterase"
                     /codon_start=1
                     /product="acetyl-coenzyme A thioesterase isoform 1"
                     /protein_id="NP_083066.1"
                     /db_xref="CCDS:CCDS26678.1"
                     /db_xref="GeneID:74156"
                     /db_xref="MGI:MGI:1921406"
                     /translation="MESMVAPGEVLMSQAIQPAHADSRGELSAGQLLKWMDTTACLAA
                     EKHAGISCVTASMDDILFEDTARIGQIITIRAKVTRAFSTSMEISIKVIVQDKFTGIQ
                     KLLCVAFSTFVAKPVGKEKVHLKPVLLQTEQEQVEHNLASERRKVRLQHENTFNNIMK
                     ESSRFSDSICNEEEGTATTMGTSVQSIELVLPPHANHHGNTFGGQIMAWMETVATISA
                     SRLCHGHPFLKSVDMFKFRGPSTVGDRLVFSAIVNNTFQNSVEVGVRVEAFDCQEWAE
                     GQGRHINSAFLIYNAVDDQEKLITFPRIQPISKDDFRRYQGAIARRRIRLGRKYVISH
                     KKEVPLSAQWDISKKGSLSNTNVEALKNLASKSGWEITTTLEKIKIYTLEEQDAISVK
                     VEKLVGSPAHIAYHLLSDLTKRPLWDPHYISCEVIDQVSEDDQIYYITCSVVNGDKPK
                     DFVVLVSRRKPLKDNNTYTVALRSVVLPSVPSSPQYIRSEVICAGFLIQAVDSNSCTV
                     TYLNQMSDSILPYFAGNIGGWSKSIEEAAASCIKFIENATPDGLKSVL"
     exon            146..215
                     /gene="Acot12"
                     /gene_synonym="1300004O04Rik; 4930449F15Rik; Cach; CACH-1;
                     mCACH-1"
                     /inference="alignment:Splign:2.1.0"
     exon            216..276
                     /gene="Acot12"
                     /gene_synonym="1300004O04Rik; 4930449F15Rik; Cach; CACH-1;
                     mCACH-1"
                     /inference="alignment:Splign:2.1.0"
     exon            277..378
                     /gene="Acot12"
                     /gene_synonym="1300004O04Rik; 4930449F15Rik; Cach; CACH-1;
                     mCACH-1"
                     /inference="alignment:Splign:2.1.0"
     exon            379..514
                     /gene="Acot12"
                     /gene_synonym="1300004O04Rik; 4930449F15Rik; Cach; CACH-1;
                     mCACH-1"
                     /inference="alignment:Splign:2.1.0"
     exon            515..671
                     /gene="Acot12"
                     /gene_synonym="1300004O04Rik; 4930449F15Rik; Cach; CACH-1;
                     mCACH-1"
                     /inference="alignment:Splign:2.1.0"
     exon            672..791
                     /gene="Acot12"
                     /gene_synonym="1300004O04Rik; 4930449F15Rik; Cach; CACH-1;
                     mCACH-1"
                     /inference="alignment:Splign:2.1.0"
     exon            792..942
                     /gene="Acot12"
                     /gene_synonym="1300004O04Rik; 4930449F15Rik; Cach; CACH-1;
                     mCACH-1"
                     /inference="alignment:Splign:2.1.0"
     exon            943..998
                     /gene="Acot12"
                     /gene_synonym="1300004O04Rik; 4930449F15Rik; Cach; CACH-1;
                     mCACH-1"
                     /inference="alignment:Splign:2.1.0"
     exon            999..1062
                     /gene="Acot12"
                     /gene_synonym="1300004O04Rik; 4930449F15Rik; Cach; CACH-1;
                     mCACH-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1063..1146
                     /gene="Acot12"
                     /gene_synonym="1300004O04Rik; 4930449F15Rik; Cach; CACH-1;
                     mCACH-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1147..1280
                     /gene="Acot12"
                     /gene_synonym="1300004O04Rik; 4930449F15Rik; Cach; CACH-1;
                     mCACH-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1281..1409
                     /gene="Acot12"
                     /gene_synonym="1300004O04Rik; 4930449F15Rik; Cach; CACH-1;
                     mCACH-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1410..1536
                     /gene="Acot12"
                     /gene_synonym="1300004O04Rik; 4930449F15Rik; Cach; CACH-1;
                     mCACH-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1537..3058
                     /gene="Acot12"
                     /gene_synonym="1300004O04Rik; 4930449F15Rik; Cach; CACH-1;
                     mCACH-1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1997..2002
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Acot12"
                     /gene_synonym="1300004O04Rik; 4930449F15Rik; Cach; CACH-1;
                     mCACH-1"
                     /note="hexamer: TATAAA"
     polyA_site      2023
                     /gene="Acot12"
                     /gene_synonym="1300004O04Rik; 4930449F15Rik; Cach; CACH-1;
                     mCACH-1"
     regulatory      3038..3043
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Acot12"
                     /gene_synonym="1300004O04Rik; 4930449F15Rik; Cach; CACH-1;
                     mCACH-1"
                     /note="hexamer: AATAAA"
     polyA_site      3058
                     /gene="Acot12"
                     /gene_synonym="1300004O04Rik; 4930449F15Rik; Cach; CACH-1;
                     mCACH-1"
                     /note="major polyA site"
ORIGIN      
        1 ctctcagcca ggacgatgga gtcgatggtg gcgccgggtg aggtgctcat gagccaggcc
       61 atccagccgg ctcatgccga ctcccgcggc gagctgagcg cagggcagct gctcaagtgg
      121 atggacacca ccgcttgcct ggcggccgaa aagcatgctg ggatttcctg tgtcacagcc
      181 tccatggatg acattctgtt tgaggacaca gcgagaattg gacaaattat taccatcaga
      241 gcaaaagtga ctagggcgtt cagcacaagc atggagatca gtatcaaggt catagtccag
      301 gacaagttca cgggcatcca gaagctcctc tgcgtggctt tctctacgtt tgtagctaaa
      361 ccagttggca aagaaaaggt tcacttaaaa cctgtcctgc ttcaaacaga gcaagaacaa
      421 gtggagcaca atctggcttc agagagaagg aaagtccgac tgcagcatga gaacaccttc
      481 aacaacatta tgaaggagag cagcaggttc agcgattcca tttgtaatga agaagaagga
      541 acggccacca ccatgggcac ctctgtccag agcatcgagc ttgtccttcc gccccacgca
      601 aaccatcacg gaaacacatt tggtggccag atcatggcat ggatggagac agtcgccacc
      661 atttctgcaa gccgcctgtg tcatgggcat ccctttctga agtctgtgga tatgtttaaa
      721 ttccggggac cgtccacagt tggagaccgc cttgtcttca gtgccatagt caacaacacc
      781 tttcagaaca gtgtggaagt cggagtgcgt gttgaggcct ttgactgtca ggagtgggcc
      841 gagggccaag gccgccacat caacagcgct tttctcattt acaatgctgt tgatgaccag
      901 gagaaactca tcacctttcc cagaatccaa cccatttcaa aggacgattt tcgccgttac
      961 cagggagcca tcgcacggag gagaattcgc ctaggcagga aatatgttat ttcccacaag
     1021 aaagaagttc cactcagtgc acagtgggat ataagcaaaa agggatccct aagtaacacc
     1081 aatgtggaag ctctcaaaaa tctggcatcc aaaagcggtt gggagattac caccaccttg
     1141 gagaagataa aaatatatac cctggaggag caagatgcca tatctgttaa ggttgaaaag
     1201 cttgtgggca gtccagccca catagcttat catctcttgt ctgacctcac aaagcgacct
     1261 ttatgggacc cccattacat atcttgtgaa gttatagacc aggtgagcga ggacgatcag
     1321 atatattaca tcacttgctc ggtggtaaat ggagacaaac ccaaggactt tgtggtgctt
     1381 gtgtcacgaa gaaagcccct caaagacaac aacacctaca ccgtggcact aaggtcagtt
     1441 gtgctgccgt ctgtcccgtc atctccacag tacatcagaa gtgaggtcat ttgtgctggg
     1501 tttctcatcc aggctgtcga cagcaattcg tgcaccgtaa cgtacctgaa ccagatgtca
     1561 gacagcatcc tcccttactt tgctggcaat attggtggct ggtcaaaatc cattgaggaa
     1621 gctgcagcct cttgtataaa attcatagag aatgctactc ctgatggact taaaagtgtg
     1681 ttataaatgg tcaactctca gctacctgta atttaattcc aaatgtgtcc ttagcccttt
     1741 tatgtggcaa gctttggggc catacaacga tttaatatca gcctaagcaa aatgcagtta
     1801 tgaagagatg aaaaaacaaa gtgtattctg gtgatttgga gccaaaccac aagatctcac
     1861 agccctactg tactgacact tggaaattgc ctggatacga tatcacatga tctataagat
     1921 ggcacaaaat tagtcaacac tatcacatct ggctctggat tacagaagct cgatgtgtat
     1981 tctgcaatga tgagtttata aaacgtgaaa tggtgggcta aaacctgatg agtgtttaat
     2041 attagtccat gggcggcaga cacctttgtc aggagtcttt gagaagatat attccatgtt
     2101 ttagaacttt aaattaggaa gcgttaaaac ataatggaat ccaatcagtt ttttaaatat
     2161 gaacgacttc acttttgtca ttttacatat attccaggta gatggccaat ttagtatcta
     2221 tttctataca cgcacacgcc aagcacctgt aaagtgtgat attagagaga gaatattaac
     2281 tgagctcttc ttcttcatat ctcttccatt agtactttat ctcctagacg tctacattta
     2341 tcattctaca ccatgatgtg tcttaactca taagaccaag cagaatgtgt caggttagcc
     2401 tccttcaaag gatgactttt acaaatggaa gaggacctcc atcagagttg agagcaggag
     2461 acaggtttgg gctatcctca ccagatctcc tctgctcccc ggcctgctcc cacatgccca
     2521 ctcaaatagt ttgaggctgg ctacagtttt tatcttactt atttgtctca aggttcagcc
     2581 gtatgtgaac tttagaccac gttctaattt tataaattac tatttatata tcataagagt
     2641 aatgcatagc atagaacatt ctagaactaa aaaacaactt caggaaatat tttaattcta
     2701 caaggtaaaa gatttagtaa taaggggctt ataagatgct gtaacttagt gttatcattt
     2761 cagacttctg aaatgaagag gcttttaagt gctatatgcg taggcttggt ctttgaattc
     2821 agtttttgaa atgttttcct gaacagaaac agaaatactt gagtaacttt agccagctta
     2881 cttgagacat agacagtatg gactccagtt tgcaggatct accttaccca ggatgggtat
     2941 aaactctcag aacgttgtca cttgtcctag gtttgtttcc atactgccta ctttggatga
     3001 agtctagttg ataagttgga gattcgcaat aacctctaat aaaattgaac catcataa
//
