LOCUS       NM_007386               4319 bp    mRNA    linear   ROD 24-MAR-2023
DEFINITION  Mus musculus aconitase 1 (Aco1), mRNA.
ACCESSION   NM_007386
VERSION     NM_007386.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4319)
  AUTHORS   Votava JA, Reese SR, Deck KM, Nizzi CP, Anderson SA, Djamali A and
            Eisenstein RS.
  TITLE     Dysregulation of the sensory and regulatory pathways controlling
            cellular iron metabolism in unilateral obstructive nephropathy
  JOURNAL   Am J Physiol Renal Physiol 322 (1), F89-F103 (2022)
   PUBMED   34843656
REFERENCE   2  (bases 1 to 4319)
  AUTHORS   Yook JS, You M, Kim J, Toney AM, Fan R, Puniya BL, Helikar T,
            Vaulont S, Deschemin JC, Okla M, Xie L, Ghosh MC, Rouault TA, Lee J
            and Chung S.
  TITLE     Essential role of systemic iron mobilization and redistribution for
            adaptive thermogenesis through HIF2-alpha/hepcidin axis
  JOURNAL   Proc Natl Acad Sci U S A 118 (40) (2021)
   PUBMED   34593646
REFERENCE   3  (bases 1 to 4319)
  AUTHORS   Ghosh MC, Zhang DL, Ollivierre WH, Noguchi A, Springer DA, Linehan
            WM and Rouault TA.
  TITLE     Therapeutic inhibition of HIF-2alpha reverses polycythemia and
            pulmonary hypertension in murine models of human diseases
  JOURNAL   Blood 137 (18), 2509-2519 (2021)
   PUBMED   33512384
REFERENCE   4  (bases 1 to 4319)
  AUTHORS   Ghosh MC, Zhang DL, Ollivierre H, Eckhaus MA and Rouault TA.
  TITLE     Translational repression of HIF2alpha expression in mice with
            Chuvash polycythemia reverses polycythemia
  JOURNAL   J Clin Invest 128 (4), 1317-1325 (2018)
   PUBMED   29480820
REFERENCE   5  (bases 1 to 4319)
  AUTHORS   Zhou S, Du X, Xie J and Wang J.
  TITLE     Interleukin-6 regulates iron-related proteins through c-Jun
            N-terminal kinase activation in BV2 microglial cell lines
  JOURNAL   PLoS One 12 (7), e0180464 (2017)
   PUBMED   28672025
  REMARK    GeneRIF: IL-6 likely up-regulates IRP1 and DMT1 expression and
            down-regulates FPN1 expression in BV2 microglial cells through JNK
            signaling pathways
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4319)
  AUTHORS   Philpott CC, Klausner RD and Rouault TA.
  TITLE     The bifunctional iron-responsive element binding protein/cytosolic
            aconitase: the role of active-site residues in ligand binding and
            regulation
  JOURNAL   Proc Natl Acad Sci U S A 91 (15), 7321-7325 (1994)
   PUBMED   8041788
  REMARK    GeneRIF: Human ACO1 is a moonlighting protein that has both
            aconitase enzyme activity as well as an mRNA binding function.
REFERENCE   7  (bases 1 to 4319)
  AUTHORS   Philpott CC, Rouault TA and Klausner RD.
  TITLE     Sequence and expression of the murine iron-responsive element
            binding protein
  JOURNAL   Nucleic Acids Res 19 (22), 6333 (1991)
   PUBMED   1956798
REFERENCE   8  (bases 1 to 4319)
  AUTHORS   Kaptain S, Downey WE, Tang C, Philpott C, Haile D, Orloff DG,
            Harford JB, Rouault TA and Klausner RD.
  TITLE     A regulated RNA binding protein also possesses aconitase activity
  JOURNAL   Proc Natl Acad Sci U S A 88 (22), 10109-10113 (1991)
   PUBMED   1946430
  REMARK    GeneRIF: Aco1 is a moonlighting protein that has aconitase activity
            as well as mRNA binding ability.
REFERENCE   9  (bases 1 to 4319)
  AUTHORS   Nadeau,J.H.
  TITLE     Length of the chromosomal segment marked by galactose-1-phosphate
            uridyl transferase and soluble aconitase and conserved since
            divergence of lineages leading to mouse and man
  JOURNAL   Genet Res 52 (2), 141-144 (1988)
   PUBMED   2850257
REFERENCE   10 (bases 1 to 4319)
  AUTHORS   Nadeau,J.H. and Eicher,E.M.
  TITLE     Conserved linkage of soluble aconitase and galactose-1-phosphate
            uridyl transferase in mouse and man: assignment of these genes to
            mouse chromosome 4
  JOURNAL   Cytogenet Cell Genet 34 (4), 271-281 (1982)
   PUBMED   6297853
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BB854442.1, BY145469.1, AK163985.1 and AL831793.4.
            
            [WARNING] On Apr 24, 2023 this sequence was replaced by
            NM_007386.3.
            
            On Jul 14, 2006 this sequence version replaced NM_007386.1.
            
            Summary: This gene encodes a member of the aconitase/IPM isomerase
            protein family. The encoded protein has been identified as a
            moonlighting protein based on its ability to perform
            mechanistically distinct functions. Depending on iron levels in the
            cytosol, the encoded protein can function as either an aconitase
            enzyme or as an mRNA binding protein. When cellular iron levels are
            high, the encoded protein functions as an aconitase, an essential
            enzyme in the TCA cycle that catalyzes the conversion of citrate to
            isocitrate. When cellular iron levels are low, the encoded protein
            regulates iron uptake and utilization by binding to iron-responsive
            elements in the untranslated regions of mRNAs for genes involved in
            iron metabolism. Disruption of this gene is associated with
            pulmonary hypertension and polycythemia. [provided by RefSeq, Jan
            2014].
            
            Sequence Note: This sequence has been modified as follows: removed
            28 bp suspected to be vector contamination from the 3' end.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660815.207336.1, AK163985.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            multifunctional gene product(s) :: PMID: 1946430
            RefSeq Select criteria          :: based on conservation,
                                               expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-156               BB854442.1         2-157
            157-202             BY145469.1         1-46
            203-3644            AK163985.1         2-3443
            3645-4319           AL831793.4         65195-65869
FEATURES             Location/Qualifiers
     source          1..4319
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 20.24 cM"
     gene            1..4319
                     /gene="Aco1"
                     /gene_synonym="Aco-1; Irebp; Irp1"
                     /note="aconitase 1"
                     /db_xref="GeneID:11428"
                     /db_xref="MGI:MGI:87879"
     exon            1..277
                     /gene="Aco1"
                     /gene_synonym="Aco-1; Irebp; Irp1"
                     /inference="alignment:Splign:2.1.0"
     exon            278..404
                     /gene="Aco1"
                     /gene_synonym="Aco-1; Irebp; Irp1"
                     /inference="alignment:Splign:2.1.0"
     CDS             308..2977
                     /gene="Aco1"
                     /gene_synonym="Aco-1; Irebp; Irp1"
                     /EC_number="4.2.1.3"
                     /note="cytoplasmic aconitase; IRE-BP 1; citrate
                     hydro-lyase; iron regulatory protein 1; iron-responsive
                     element-binding protein 1"
                     /codon_start=1
                     /product="cytoplasmic aconitate hydratase"
                     /protein_id="NP_031412.2"
                     /db_xref="CCDS:CCDS18042.1"
                     /db_xref="GeneID:11428"
                     /db_xref="MGI:MGI:87879"
                     /translation="MKNPFAHLAEPLDAAQPGKRFFNLNKLEDSRYGRLPFSIRVLLE
                     AAVRNCDEFLVKKNDIENILNWNVMQHKNIEVPFKPARVILQDFTGVPAVVDFAAMRD
                     AVKKLGGNPEKINPVCPADLVIDHSIQVDFNRRADSLQKNQDLEFERNKERFEFLKWG
                     SQAFCNMRIIPPGSGIIHQVNLEYLARVVFDQDGCYYPDSLVGTDSHTTMIDGLGVLG
                     WGVGGIEAEAVMLGQPISMVLPQVIGYKLMGKPHPLVTSTDIVLTITKHLRQVGVVGK
                     FVEFFGPGVAQLSIADRATIANMCPEYGATAAFFPVDEVSIAYLLQTGREEDKVKHIQ
                     KYLQAVGMFRDFNDTSQDPDFTQVVELDLKTVVPCCSGPKRPQDKVAVSEMKKDFESC
                     LGAKQGFKGFQVAPDRHNDRKTFLYSNSEFTLAHGSVVIAAITSCTNTSNPSVMLGAG
                     LLAKKAVEAGLSVKPYIKTSLSPGSGVVTYYLRESGVMPYLSQLGFDVVGYGCMTCIG
                     NSGPLPEPVVEAITQGDLVAVGVLSGNRNFEGRVHPNTRANYLASPPLVIAYAIAGTV
                     RIDFEKEPLGVNAQGRQVFLKDIWPTRDEIQAVERQHVIPGMFKEVYQKIETVNKSWN
                     ALAAPSEKLYAWNPKSTYIKSPPFFESLTLDLQPPKSIVDAYVLLNLGDSVTTDHISP
                     AGNIARNSPAARYLTNRGLTPREFNSYGSRRGNDAIMARGTFANIRLLNKFLNKQAPQ
                     TVHLPSGETLDVFDAAERYQQAGLPLIVLAGKEYGSGSSRDWAAKGPFLLGIKAVLAE
                     SYERIHRSNLVGMGVIPLEYLPGETADSLGLTGRERYTINIPEDLKPRMTVQIKLDTG
                     KTFQAVMRFDTDVELTYFHNGGILNYMIRKMAQ"
     exon            405..573
                     /gene="Aco1"
                     /gene_synonym="Aco-1; Irebp; Irp1"
                     /inference="alignment:Splign:2.1.0"
     exon            574..711
                     /gene="Aco1"
                     /gene_synonym="Aco-1; Irebp; Irp1"
                     /inference="alignment:Splign:2.1.0"
     exon            712..781
                     /gene="Aco1"
                     /gene_synonym="Aco-1; Irebp; Irp1"
                     /inference="alignment:Splign:2.1.0"
     exon            782..965
                     /gene="Aco1"
                     /gene_synonym="Aco-1; Irebp; Irp1"
                     /inference="alignment:Splign:2.1.0"
     exon            966..1105
                     /gene="Aco1"
                     /gene_synonym="Aco-1; Irebp; Irp1"
                     /inference="alignment:Splign:2.1.0"
     exon            1106..1277
                     /gene="Aco1"
                     /gene_synonym="Aco-1; Irebp; Irp1"
                     /inference="alignment:Splign:2.1.0"
     exon            1278..1378
                     /gene="Aco1"
                     /gene_synonym="Aco-1; Irebp; Irp1"
                     /inference="alignment:Splign:2.1.0"
     exon            1379..1495
                     /gene="Aco1"
                     /gene_synonym="Aco-1; Irebp; Irp1"
                     /inference="alignment:Splign:2.1.0"
     exon            1496..1655
                     /gene="Aco1"
                     /gene_synonym="Aco-1; Irebp; Irp1"
                     /inference="alignment:Splign:2.1.0"
     exon            1656..1791
                     /gene="Aco1"
                     /gene_synonym="Aco-1; Irebp; Irp1"
                     /inference="alignment:Splign:2.1.0"
     exon            1792..1876
                     /gene="Aco1"
                     /gene_synonym="Aco-1; Irebp; Irp1"
                     /inference="alignment:Splign:2.1.0"
     exon            1877..2033
                     /gene="Aco1"
                     /gene_synonym="Aco-1; Irebp; Irp1"
                     /inference="alignment:Splign:2.1.0"
     exon            2034..2158
                     /gene="Aco1"
                     /gene_synonym="Aco-1; Irebp; Irp1"
                     /inference="alignment:Splign:2.1.0"
     exon            2159..2263
                     /gene="Aco1"
                     /gene_synonym="Aco-1; Irebp; Irp1"
                     /inference="alignment:Splign:2.1.0"
     exon            2264..2406
                     /gene="Aco1"
                     /gene_synonym="Aco-1; Irebp; Irp1"
                     /inference="alignment:Splign:2.1.0"
     exon            2407..2554
                     /gene="Aco1"
                     /gene_synonym="Aco-1; Irebp; Irp1"
                     /inference="alignment:Splign:2.1.0"
     exon            2555..2677
                     /gene="Aco1"
                     /gene_synonym="Aco-1; Irebp; Irp1"
                     /inference="alignment:Splign:2.1.0"
     exon            2678..2863
                     /gene="Aco1"
                     /gene_synonym="Aco-1; Irebp; Irp1"
                     /inference="alignment:Splign:2.1.0"
     exon            2864..4319
                     /gene="Aco1"
                     /gene_synonym="Aco-1; Irebp; Irp1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3627..3632
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Aco1"
                     /gene_synonym="Aco-1; Irebp; Irp1"
                     /note="putative"
     polyA_site      3644
                     /gene="Aco1"
                     /gene_synonym="Aco-1; Irebp; Irp1"
                     /note="putative"
ORIGIN      
        1 ggaaggttcc gaagggaggg cgcacccgga ctagactgca agcttcagcc atcgcttgca
       61 gcttctagag ggaggaggtg cccaggctcc gcccctcggg gggcggagtg aggcccgggc
      121 cccgcccctc tcccgcagca gctggttccg gcccgctctc caccggtccc gcctcgctgc
      181 cggagcgtcc ggaggaccgc tcagtgggag gagcgcggag tcggcctggg acattgctag
      241 cacccgcctg tgtgctccgg gaactgcact gtgagcgttt ttcagagaca cgtgaccatc
      301 aataagcatg aagaacccat ttgcacacct tgctgagccc ttggacgctg cccaaccagg
      361 aaagaggttc ttcaacttga ataaattgga ggactcaaga tacggacgct taccattttc
      421 tatcagagtt ctcctggagg cagccgttcg gaactgtgat gagtttctgg tgaagaaaaa
      481 tgacatcgag aatatcctga attggaatgt catgcaacat aagaacatag aagtgccgtt
      541 taagccagcc cgagtcatac tgcaagactt tacgggtgtg cctgctgtgg tggattttgc
      601 agcaatgcgc gatgctgtga agaagttggg agggaatccg gagaaaatta accctgtctg
      661 ccccgctgac cttgtaattg atcattccat ccaggttgat ttcaacagaa gggcagacag
      721 tttacagaag aatcaagacc tggagtttga aagaaataaa gaacgatttg aatttctaaa
      781 gtggggttcc caggcctttt gcaacatgcg gataatccct cctggctcgg gaatcattca
      841 ccaagtgaac ctggagtatt tggcaagagt agtgtttgat caggatggat gctactaccc
      901 agacagcctc gtgggcacag attcacacac gaccatgatt gacggcctgg gagttcttgg
      961 ttggggtgtg ggtggtattg aagcagaagc tgtcatgctg ggtcagccca tcagcatggt
     1021 gcttccccag gtgattggct acaagctgat ggggaagcct caccctctgg tgacatccac
     1081 ggacatcgtg ctcaccatta ccaagcacct ccgacaagtg ggagttgtgg gcaaatttgt
     1141 ggagtttttc gggccaggag tggctcagct gtccattgct gaccgagcta cgattgccaa
     1201 tatgtgccca gagtatggtg cgacagcagc cttcttcccg gttgatgaag ttagcatcgc
     1261 gtacctgctg cagacaggcc gcgaggaaga caaagtcaag cacattcaga agtatcttca
     1321 ggctgtaggc atgtttcgag atttcaacga cacctctcaa gacccagact tcactcaggt
     1381 tgtggagtta gatctgaaaa cagttgtgcc ttgctgcagt ggacccaaaa gacctcagga
     1441 caaagtcgcg gtgtctgaga tgaaaaagga ctttgaaagc tgccttggag ccaagcaagg
     1501 atttaaaggt ttccaagttg ctccagaccg tcacaatgac cgcaagacgt tcctctatag
     1561 taacagtgaa ttcactctcg ctcatggctc tgtggtaatc gctgccatca ctagctgcac
     1621 aaacaccagc aatccatccg tgatgttagg agcaggattg ttagcaaaga aagctgtgga
     1681 ggcggggctg agtgtgaagc cttacatcaa aaccagcctg tctcctggaa gtggagtggt
     1741 cacctactac cttcgagaga gtggagtcat gccttacctg tcccagttag ggtttgatgt
     1801 ggtgggctac ggctgcatga cctgcatcgg caacagtgga ccccttcctg aacctgtggt
     1861 cgaggcaatc acccagggag acctcgtagc tgttggcgtt ctgtctggga ataggaattt
     1921 tgaaggccga gtccatccta acacacgagc caactactta gcatcccccc ctctggtaat
     1981 agcatatgcg attgcaggca ccgtcaggat tgacttcgag aaagagcctt tgggagtgaa
     2041 cgcacagggc cggcaagtgt ttctgaagga catctggccc acgcgagatg agatccaggc
     2101 ggtggaaagg cagcatgtca tccccgggat gtttaaggag gtctatcaga aaatagagac
     2161 tgtaaacaaa agctggaatg ccttagcagc cccgtcagag aagctgtatg cgtggaatcc
     2221 caaatctact tacattaagt cgccaccatt ctttgaaagc ttgactttag atctccagcc
     2281 acccaagtcc atagtggatg cctatgtgct actgaattta ggagattcag taacaacgga
     2341 ccatatctct cccgccggca atattgcaag aaacagccct gcggctcgct acttgacgaa
     2401 cagaggccta acaccacgag agttcaactc ctatggctcc cgccggggta acgacgccat
     2461 catggcccgg gggacatttg ccaacattcg cttgctgaac aagtttctga acaagcaggc
     2521 acctcagact gtccatctcc cctcaggaga aacccttgat gtattcgacg ctgctgagcg
     2581 gtaccagcag gctggacttc ccctgattgt tctggctggg aaagaatacg gttcaggcag
     2641 ctcccgagac tgggcagcca aaggcccttt cctgctggga atcaaagctg tcctggcaga
     2701 gagctatgag cgcattcacc gcagcaactt ggttggcatg ggggtgatcc cccttgagta
     2761 tctccctggt gaaactgcag actctctggg actcacaggt cgggaaagat acactatcaa
     2821 catccctgaa gacctcaagc ctcgcatgac ggtgcagatc aagctggaca ctgggaagac
     2881 cttccaggcc gtgatgaggt tcgacactga tgtggagctc acgtacttcc acaatggagg
     2941 catcctgaac tacatgatcc gaaagatggc ccagtaggtg ctgacctctc aggagacccg
     3001 cacttggtgc tagacccaat gaggaaccag gcccccactg gtggaggcct ggcagagcag
     3061 ccacatctac ttctgatgag ggtgctggca ggatgagcaa gtgggcactg ccattcctgg
     3121 aggcacagag ccaggagtct ctagttttgt gatttgttca tctttttatc cctttctgta
     3181 atctggattc tagaatcacg ggaaggtccg tagtaccaaa gagaactcgc ttctctttaa
     3241 agtcacggac attgattttt ttccctctga ctaatcttga gctcagcact agccagtatt
     3301 ctcagaagtg gctcctaccc tttctgttgt tctgtgtttg tctctgctcg gtgacaccct
     3361 tccctggaga gcccagtcct ccgtgtatta caccagtgtt aactgacata gcatagacct
     3421 tcttcacact tcagattcat agcagcgatg ggatcccttc ctttgaagtg aacaagtcct
     3481 tgtagatagg tcacccgtca aaagtgtttg attatctacc tttcaatcaa catgagattg
     3541 cctcttacca ttttcaacga ttgttgacag gggtcctttg tttgaaaata actggggaga
     3601 gatacgggat ttctaatttg aataagatta aatatatttt catgaaaacg tgttggcttt
     3661 ggagttgagt tctgtcgggg gaatgtcgcg acactgagag ccccggaagt ccttcgagtc
     3721 cttcgagtct gctgtttgta cccctgcgtc tgagctgcgg gcacagaggg aacactgtgg
     3781 agccagcgtg tgagcagcta gtgtcagcgc aaaggacaag aaagcacctt ctgtcttggt
     3841 cacagcagca gcaaagagca tatcgcaact tcttggtgcc ctaaaatctc ttcactgatc
     3901 ttcataactg gaattatttt agggtgtgcc ttattctgct caggctgctg tagcaaaatg
     3961 cctgagtaag aaccagaact gagaactgag ctgtgagtga cccaaaccct gagccttgtc
     4021 cagaccaccc agtgcctgtg atggagtaaa cattccgctg ggggaggggt tgctttgtct
     4081 ctcttaacgt tttatctttt ctttccttct tttctttttc tttttttctt tctttctttc
     4141 ttcttttttg ttgttgatgt tgttgttgct gttgttgttt ggtttttttc gagacagggt
     4201 ttctctgtat agccctggct gtcctggaac tcactctgtc agccaggctg gccttgaact
     4261 cagaaatcca cctgcctctg cctcccaagt gctggattaa aggtgtgcgc caccaccgc
//
