LOCUS       NM_009599               2224 bp    mRNA    linear   ROD 24-DEC-2024
DEFINITION  Mus musculus acetylcholinesterase (Ache), transcript variant 2,
            mRNA.
ACCESSION   NM_009599
VERSION     NM_009599.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2224)
  AUTHORS   Eguchi,T., Tezuka,T., Watanabe,Y., Inoue-Yamauchi,A., Sagara,H.,
            Ozawa,M. and Yamanashi,Y.
  TITLE     Calcium-binding protein 7 expressed in muscle negatively regulates
            age-related degeneration of neuromuscular junctions in mice
  JOURNAL   iScience 27 (2), 108997 (2024)
   PUBMED   38327785
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2224)
  AUTHORS   Virtanen,H.T., Choopanian,P., Porokuokka,L.L., Forsgard,R.,
            Garton,D.R., Olfat,S., Korpela,R., Mirzaie,M. and Andressoo,J.O.
  TITLE     Interindividual Variation in Gut Nitrergic Neuron Density Is
            Regulated By GDNF Levels and ETV1
  JOURNAL   Cell Mol Gastroenterol Hepatol 18 (6), 101405 (2024)
   PUBMED   39299667
REFERENCE   4  (bases 1 to 2224)
  AUTHORS   Cheffer,A., Garcia-Miralles,M., Maier,E., Akol,I., Franz,H.,
            Srinivasan,V.S.V. and Vogel,T.
  TITLE     DOT1L deletion impairs the development of cortical
            parvalbumin-expressing interneurons
  JOURNAL   Cereb Cortex 33 (19), 10272-10285 (2023)
   PUBMED   37566909
REFERENCE   5  (bases 1 to 2224)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2224)
  AUTHORS   Randall,W.R.
  TITLE     Cellular expression of a cloned, hydrophilic, murine
            acetylcholinesterase. Evidence of palmitoylated membrane-bound
            forms
  JOURNAL   J Biol Chem 269 (16), 12367-12374 (1994)
   PUBMED   7512968
REFERENCE   7  (bases 1 to 2224)
  AUTHORS   Misawa,H., Ishii,K. and Deguchi,T.
  TITLE     Gene expression of mouse choline acetyltransferase. Alternative
            splicing and identification of a highly active promoter region
  JOURNAL   J Biol Chem 267 (28), 20392-20399 (1992)
   PUBMED   1400357
REFERENCE   8  (bases 1 to 2224)
  AUTHORS   Rachinsky,T.L., Crenshaw,E.B. 3rd and Taylor,P.
  TITLE     Assignment of the gene for acetylcholinesterase to distal mouse
            chromosome 5
  JOURNAL   Genomics 14 (2), 511-514 (1992)
   PUBMED   1358806
REFERENCE   9  (bases 1 to 2224)
  AUTHORS   Li,Y., Camp,S., Rachinsky,T.L., Getman,D. and Taylor,P.
  TITLE     Gene structure of mammalian acetylcholinesterase. Alternative exons
            dictate tissue-specific expression
  JOURNAL   J Biol Chem 266 (34), 23083-23090 (1991)
   PUBMED   1744105
REFERENCE   10 (bases 1 to 2224)
  AUTHORS   Rachinsky,T.L., Camp,S., Li,Y., Ekstrom,T.J., Newton,M. and
            Taylor,P.
  TITLE     Molecular cloning of mouse acetylcholinesterase: tissue
            distribution of alternatively spliced mRNA species
  JOURNAL   Neuron 5 (3), 317-327 (1990)
   PUBMED   2400605
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY261443.1, AK042335.1 and BQ175376.1.
            
            On Feb 19, 2014 this sequence version replaced NM_009599.3.
            
            Transcript Variant: This variant (2) uses an alternate splice site
            in the 5' UTR compared to variant 1. Both variants 1 and 2 encode
            the same protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK042335.1, AK043748.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849377
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-23                BY261443.1         1-23
            24-2206             AK042335.1         2-2184
            2207-2224           BQ175376.1         1-18                c
FEATURES             Location/Qualifiers
     source          1..2224
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 76.32 cM"
     gene            1..2224
                     /gene="Ache"
                     /note="acetylcholinesterase"
                     /db_xref="GeneID:11423"
                     /db_xref="MGI:MGI:87876"
     exon            1..114
                     /gene="Ache"
                     /inference="alignment:Splign:2.1.0"
     exon            115..1204
                     /gene="Ache"
                     /inference="alignment:Splign:2.1.0"
     CDS             137..1981
                     /gene="Ache"
                     /EC_number="3.1.1.7"
                     /codon_start=1
                     /product="acetylcholinesterase precursor"
                     /protein_id="NP_033729.1"
                     /db_xref="CCDS:CCDS19763.1"
                     /db_xref="GeneID:11423"
                     /db_xref="MGI:MGI:87876"
                     /translation="MRPPWYPLHTPSLAFPLLFLLLSLLGGGARAEGREDPQLLVRVR
                     GGQLRGIRLKAPGGPVSAFLGIPFAEPPVGSRRFMPPEPKRPWSGVLDATTFQNVCYQ
                     YVDTLYPGFEGTEMWNPNRELSEDCLYLNVWTPYPRPASPTPVLIWIYGGGFYSGAAS
                     LDVYDGRFLAQVEGAVLVSMNYRVGTFGFLALPGSREAPGNVGLLDQRLALQWVQENI
                     AAFGGDPMSVTLFGESAGAASVGMHILSLPSRSLFHRAVLQSGTPNGPWATVSAGEAR
                     RRATLLARLVGCPPGGAGGNDTELIACLRTRPAQDLVDHEWHVLPQESIFRFSFVPVV
                     DGDFLSDTPEALINTGDFQDLQVLVGVVKDEGSYFLVYGVPGFSKDNESLISRAQFLA
                     GVRIGVPQASDLAAEAVVLHYTDWLHPEDPTHLRDAMSAVVGDHNVVCPVAQLAGRLA
                     AQGARVYAYIFEHRASTLTWPLWMGVPHGYEIEFIFGLPLDPSLNYTTEERIFAQRLM
                     KYWTNFARTGDPNDPRDSKSPQWPPYTTAAQQYVSLNLKPLEVRRGLRAQTCAFWNRF
                     LPKLLSATDTLDEAERQWKAEFHRWSSYMVHWKNQFDHYSKQERCSDL"
     exon            1205..1689
                     /gene="Ache"
                     /inference="alignment:Splign:2.1.0"
     exon            1690..1859
                     /gene="Ache"
                     /inference="alignment:Splign:2.1.0"
     exon            1860..2209
                     /gene="Ache"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2187..2192
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ache"
                     /note="hexamer: AATAAA"
     polyA_site      2204
                     /gene="Ache"
                     /note="major polyA site"
ORIGIN      
        1 ggtgtgtgcg gggggccgga ggcggctgtc actgtcggct cagcctgcgc cggggaacat
       61 tggccgcctc cagctcccgg cgcggcccga cccggcccgg cttggccgcc tcagacacca
      121 gcctgtcctg gcagtcatga ggcctccctg gtatcccctg catacacctt ccctggcttt
      181 tccactcctc ttcctcctcc tctccctcct gggaggaggg gcaagggctg agggccggga
      241 agacccgcag ctgctggtga gggttcgagg gggccagctg aggggcatcc gcctgaaggc
      301 ccctggaggc ccagtctcag cttttctggg catccccttt gcagagccac ctgtgggctc
      361 acgtagattt atgccaccag agcccaagcg gccctggtca ggagtgttgg atgctaccac
      421 cttccaaaat gtctgctacc agtacgtgga caccctgtac cctgggtttg agggtactga
      481 gatgtggaac cccaaccgag agttgagtga agactgcctg tatcttaatg tgtggacacc
      541 ataccccaga cctgcttctc ccacacctgt cctcatctgg atctatgggg gtggtttcta
      601 cagcggagcg gcctccttgg atgtgtatga cggccgtttc ctggcccagg ttgagggagc
      661 tgtgttggta tctatgaact accgagtggg aacctttggc ttcttggccc taccaggaag
      721 cagagaagcc cctggcaatg taggtctgct ggatcaacgg cttgccttgc aatgggtgca
      781 agaaaatatt gcagcctttg ggggcgaccc gatgtcagtg actctgtttg gggagagtgc
      841 gggtgcagcc tccgtgggca tgcacatact gtccctgccc agcaggagcc tcttccacag
      901 ggctgtcctc cagagtggca cacccaatgg gccctgggcc actgtgagtg ctggagaggc
      961 caggcgcagg gccacactgc tggcccgcct tgtgggctgt cccccaggtg gcgctggtgg
     1021 caatgacacc gagctgatag cctgcttgag gacaaggccc gctcaggacc tggtggacca
     1081 cgagtggcac gtcctgcctc aagaaagtat cttccgattt tccttcgtgc ctgtggtaga
     1141 cggggacttc ctcagtgaca caccggaggc tctcatcaat actggagatt ttcaagacct
     1201 gcaggtgctg gtgggtgtgg tgaaggacga gggctcctac tttctggttt acggggtccc
     1261 aggcttcagc aaagacaatg aatctctcat cagccgggcc cagttcctgg ctggggtgcg
     1321 gatcggtgta ccccaagcaa gtgacctggc agccgaggct gtggtcctgc attacacaga
     1381 ctggctgcac cctgaggacc ctactcacct gagagatgcc atgagtgcag tggtaggcga
     1441 ccacaacgtt gtgtgccctg tggcccagct ggctgggcga ctggctgccc aaggggcccg
     1501 ggtctatgcc tacatctttg aacaccgtgc ctccacactg acttggcccc tctggatggg
     1561 ggtgccccat ggctatgaaa tcgagttcat ctttgggctc cccctggatc cctcgctgaa
     1621 ctacaccacg gaggagagga tctttgctca gcgacttatg aaatactgga ccaattttgc
     1681 ccgcacaggg gaccccaatg accctcgaga ctccaaatct ccacagtggc caccgtacac
     1741 cactgccgcg cagcaatatg tgagcctgaa cctgaagccc ttagaggtgc ggcggggact
     1801 gcgcgcccag acctgcgcct tctggaatcg ctttctcccc aaattgctca gcgccaccga
     1861 tactctggac gaggcggagc gccagtggaa ggccgagttc caccgctgga gctcctacat
     1921 ggtgcactgg aagaaccagt tcgaccacta tagcaagcag gagcgctgct cagacctgtg
     1981 accccttggg ggaccccagg tcctgccgcc ctgcccgagc ccctagctgt atatacacta
     2041 tttatttaag ggctgggata taatacgacc gagcccccag gccctgtcca ctcctccccg
     2101 acttcctccc actaggggct ccccatcttc tgcatgtctt gggctaagct cccctccccg
     2161 cggtgccttc gcccctctgg gccgccaata aactgttaca gccaccagaa aaaaaaaaaa
     2221 aaaa
//
