LOCUS       NM_001361500            7726 bp    mRNA    linear   ROD 04-MAR-2025
DEFINITION  Mus musculus aggrecan (Acan), transcript variant 1, mRNA.
ACCESSION   NM_001361500 XM_006540566
VERSION     NM_001361500.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 7726)
  AUTHORS   Bian,F., Hansen,V., Feng,H.C., He,J., Chen,Y., Feng,K.,
            Ebrahimi,B., Gray,R.S., Chai,Y., Wu,C.L. and Liu,Z.
  TITLE     The G protein-coupled receptor ADGRG6 maintains mouse growth plate
            homeostasis through IHH signaling
  JOURNAL   J Bone Miner Res 39 (11), 1644-1658 (2024)
   PUBMED   39236220
REFERENCE   2  (bases 1 to 7726)
  AUTHORS   Kuczynski-Noyau,L., Karmann,S., Alberton,P., Martinez-Corral,I.,
            Nampoothiri,S., Sauve,F., Lhomme,T., Quarta,C., Apte,S.S.,
            Bouret,S., Aszodi,A., Rasika,S., Ciofi,P., Dam,J., Prevot,V. and
            Mattot,V.
  TITLE     A plastic aggrecan barrier modulated by peripheral energy state
            gates metabolic signal access to arcuate neurons
  JOURNAL   Nat Commun 15 (1), 6701 (2024)
   PUBMED   39112471
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 7726)
  AUTHORS   Tewari,B.P., Woo,A.M., Prim,C.E., Chaunsali,L., Patel,D.C.,
            Kimbrough,I.F., Engel,K., Browning,J.L., Campbell,S.L. and
            Sontheimer,H.
  TITLE     Astrocytes require perineuronal nets to maintain synaptic
            homeostasis in mice
  JOURNAL   Nat Neurosci 27 (8), 1475-1488 (2024)
   PUBMED   39020018
REFERENCE   4  (bases 1 to 7726)
  AUTHORS   Garcia,S.A., Wilson,K., Tang,N., Tian,H., Oichi,T.,
            Gunawardena,A.T., Chorny,M., Alferiev,I.S., Herzenberg,J.E.,
            Ng,V.Y., Iwamoto,M. and Enomoto-Iwamoto,M.
  TITLE     Analysis of the Actions of RARgamma Agonists on Growing
            Osteochondromas in a Mouse Model
  JOURNAL   Int J Mol Sci 25 (14), 7610 (2024)
   PUBMED   39062860
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 7726)
  AUTHORS   Tan,Z., Chen,P., Dong,X., Guo,S., Leung,V.Y.L., Cheung,J.P.Y.,
            Chan,D., Richardson,S.M., Hoyland,J.A., To,M.K.T. and Cheah,K.S.E.
  TITLE     Progenitor-like cells contributing to cellular heterogeneity in the
            nucleus pulposus are lost in intervertebral disc degeneration
  JOURNAL   Cell Rep 43 (6), 114342 (2024)
   PUBMED   38865240
REFERENCE   6  (bases 1 to 7726)
  AUTHORS   Brown,K.S., Yamada,Y., Abramczuk,J. and Kimata,K.
  TITLE     New genetic approaches to craniofacial growth and malformation in
            the mouse
  JOURNAL   J Craniofac Genet Dev Biol 11 (4), 357-365 (1991)
   PUBMED   1812135
  REMARK    Review article
REFERENCE   7  (bases 1 to 7726)
  AUTHORS   Tsukahara,T., Okamura,M., Suzuki,S., Iwata,H., Miura,T. and
            Kimata,K.
  TITLE     Enhanced expression of fibronectin by cmd/cmd chondrocytes and its
            modulation by exogenously added proteoglycan
  JOURNAL   J Cell Sci 100 (Pt 2), 387-395 (1991)
   PUBMED   1757493
REFERENCE   8  (bases 1 to 7726)
  AUTHORS   Yoo,T.J., Cho,H. and Yamada,Y.
  TITLE     Hearing impairment in mice with the cmd/cmd (cartilage matrix
            deficiency) mutant gene
  JOURNAL   Ann N Y Acad Sci 630, 265-267 (1991)
   PUBMED   1952601
REFERENCE   9  (bases 1 to 7726)
  AUTHORS   Houghton,M.J., Carey,J.C. and Seegmiller,R.E.
  TITLE     Pulmonary hypoplasia in mice homozygous for the cartilage matrix
            deficiency (cmd) gene: a model for human congenital disorder
  JOURNAL   Pediatr Pathol 9 (5), 501-512 (1989)
   PUBMED   2682579
REFERENCE   10 (bases 1 to 7726)
  AUTHORS   Rittenhouse,E., Dunn,L.C., Cookingham,J., Calo,C., Spiegelman,M.,
            Dooher,G.B. and Bennett,D.
  TITLE     Cartilage matrix deficiency (cmd): a new autosomal recessive lethal
            mutation in the mouse
  JOURNAL   J Embryol Exp Morphol 43, 71-84 (1978)
   PUBMED   632744
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC110520.12.
            
            On Mar 28, 2018 this sequence version replaced XM_006540566.1.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMN00849374,
                              SAMN01164131 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-383               AC110520.12        26021-26403
            384-460             AC110520.12        54983-55059
            461-844             AC110520.12        57262-57645
            845-1019            AC110520.12        59057-59231
            1020-1147           AC110520.12        60973-61100
            1148-1441           AC110520.12        62398-62691
            1442-1846           AC110520.12        63734-64138
            1847-2021           AC110520.12        65108-65282
            2022-2149           AC110520.12        65474-65601
            2150-2443           AC110520.12        66777-67070
            2444-2659           AC110520.12        69462-69677
            2660-6142           AC110520.12        70547-74029
            6143-6256           AC110520.12        80910-81023
            6257-6415           AC110520.12        84089-84247
            6416-6498           AC110520.12        84750-84832
            6499-6643           AC110520.12        85469-85613
            6644-6826           AC110520.12        86450-86632
            6827-7726           AC110520.12        86995-87894
FEATURES             Location/Qualifiers
     source          1..7726
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 44.88 cM"
     gene            1..7726
                     /gene="Acan"
                     /gene_synonym="Agc; Agc1; b2b183Clo; cmd; CSPCP; Cspg1"
                     /note="aggrecan"
                     /db_xref="GeneID:11595"
                     /db_xref="MGI:MGI:99602"
     exon            1..383
                     /gene="Acan"
                     /gene_synonym="Agc; Agc1; b2b183Clo; cmd; CSPCP; Cspg1"
                     /inference="alignment:Splign:2.1.0"
     exon            384..460
                     /gene="Acan"
                     /gene_synonym="Agc; Agc1; b2b183Clo; cmd; CSPCP; Cspg1"
                     /inference="alignment:Splign:2.1.0"
     CDS             391..6903
                     /gene="Acan"
                     /gene_synonym="Agc; Agc1; b2b183Clo; cmd; CSPCP; Cspg1"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; aggrecan 1; aggrecan core protein;
                     cartilage-specific proteoglycan core protein"
                     /codon_start=1
                     /product="aggrecan core protein isoform 1 precursor"
                     /protein_id="NP_001348429.1"
                     /db_xref="GeneID:11595"
                     /db_xref="MGI:MGI:99602"
                     /translation="MTTLLLVFVTLRVIAAVISEEVPDHDNSLSVSIPQPSPLKVLLG
                     SSLTIPCYFIDPMHPVTTAPSTAPLTPRIKWSRVSKEKEVVLLVATEGQVRVNSIYQD
                     KVSLPNYPAIPSDATLEIQNLRSNDSGIYRCEVMHGIEDSEATLEVIVKGIVFHYRAI
                     STRYTLDFDRAQRACLQNSAIIATPEQLQAAYEDGFHQCDAGWLADQTVRYPIHTPRE
                     GCYGDKDEFPGVRTYGIRDTNETYDVYCFAEEMEGEVFYATSPEKFTFQEAANECRRL
                     GARLATTGQLYLAWQGGMDMCSAGWLADRSVRYPISKARPNCGGNLLGVRTVYLHANQ
                     TGYPDPSSRYDAICYTGEDFVDIPENFFGVGGEDDITIQTVTWPDLELPLPRNVTEGE
                     ALGSVILTAKPIFDLSPTISEPGEALTLAPEVGSTAFPEAEERTGEATRPWGFPAEVT
                     RGPDSATAFASEDLVVRVTISPGAAEVPGQPRLPGGVVFHYRPGSTRYSLTFEEAQQA
                     CMHTGAVIASPEQLQAAYEAGYEQCDAGWLQDQTVRYPIVSPRTPCVGDKDSSPGVRT
                     YGVRPSSETYDVYCYVDKLEGEVFFATRLEQFTFQEARAFCAAQNATLASTGQLYAAW
                     SQGLDKCYAGWLADGTLRYPIITPRPACGGDKPGVRTVYLYPNQTGLPDPLSKHHAFC
                     FRGVSVAPSPGEEGGSTPTSPSDIEDWIVTQVGPGVDAVPLEPKTTEVPYFTTEPRKQ
                     TEWEPAYTPVGTSPQPGIPPTWLPTLPAAEEHTESPSASEEPSASAVPSTSEEPYTSS
                     FAVPSMTELPGSGEASGAPDLSGDFTGSGDASGRLDSSGQPSGGIESGLPSGDLDSSG
                     LSPTVSSGLPVESGSASGDGEVPWSHTPTVGRLPSGGESPEGSASASGTGDLSGLPSG
                     GEITETSTSGAEETSGLPSGGDGLETSTSGVDDVSGIPTGREGLETSASGVEDLSGLP
                     SGEEGSETSTSGIEDISVLPTGGESLETSASGVGDLSGLPSGGESLETSASGAEDVTQ
                     LPTERGGLETSASGVEDITVLPTGRESLETSASGVEDVSGLPSGREGLETSASGIEDI
                     SVFPTEAEGLDTSASGGYVSGIPSGGDGTETSASGVEDVSGLPSGGEGLETSASGVED
                     LGPSTRDSLETSASGVDVTGFPSGRGDPETSVSGVGDDFSGLPSGKEGLETSASGAED
                     LSGLPSGKEDLVGSASGALDFGKLPPGTLGSGQTPEVNGFPSGFSGEYSGADIGSGPS
                     SGLPDFSGLPSGFPTVSLVDSTLVEVITATTSSELEGRGTIGISGSGEVSGLPLGELD
                     SSADISGLPSGTELSGQASGSPDSSGETSGFFDVSGQPFGSSGVSEETSGIPEISGQP
                     SGTPDTTATSGVTELNELSSGQPDVSGDGSGILFGSGQSSGITSVSGETSGISDLSGQ
                     PSGFPVFSGTATRTPDLASGTISGSGESSGITFVDTSFVEVTPTTFREEEGLGSVELS
                     GFPSGETELSGTSGTVDVSEQSSGAIDSSGLTSPTPEFSGLPSGVAEVSGEFSGVETG
                     SSLPSGAFDGSGLVSGFPTVSLVDRTLVESITQAPTAQEAGEGPSGILEFSGAHSGTP
                     DISGELSGSLDLSTLQSGQMETSTETPSSPYFSGDFSSTTDVSGESIAATTGSGESSG
                     LPEVTLNTSELVEGVTEPTVSQELGHGPSMTYTPRLFEASGDASASGDLGGAVTNFPG
                     SGIEASVPEASSDLSAYPEAGVGVSAAPEASSKLSEFPDLHGITSAFHETDLEMTTPS
                     TEVNSNPWTFQEGTREGSAAPEVSGESSTTSDIDTGTSGVPSATPMASGDRTEISGEW
                     SDHTSEVNVAISSTITESEWAQPTRYPTETLQEIESPNPSYSGEETQTAETTMSLTDA
                     PTLSSSEGSGETESTVADIDECLSSPCLNGATCVDALDTFTCLCLPSYRGNLCEIDQE
                     QCEEGWTKFQGHCYRHFHDRETWVDAERRCREQQSHLSSIVTPEEQEFVNKNAQDYQW
                     IGLNDRTIEGDFRWSDGHSLQFEKWRPNQPDNFFATGEDCVVMIWHERGEWNDVPCNY
                     QLPFTCKKGTVACGDPPVVEHARTLGQKKDRYEISSLVRYQCTEGFVQRHVPTIRCQP
                     SGHWEEPRITCTDPNTYKHRLQKRSMRPTRRSRPSMAH"
     exon            461..844
                     /gene="Acan"
                     /gene_synonym="Agc; Agc1; b2b183Clo; cmd; CSPCP; Cspg1"
                     /inference="alignment:Splign:2.1.0"
     exon            845..1019
                     /gene="Acan"
                     /gene_synonym="Agc; Agc1; b2b183Clo; cmd; CSPCP; Cspg1"
                     /inference="alignment:Splign:2.1.0"
     exon            1020..1147
                     /gene="Acan"
                     /gene_synonym="Agc; Agc1; b2b183Clo; cmd; CSPCP; Cspg1"
                     /inference="alignment:Splign:2.1.0"
     exon            1148..1441
                     /gene="Acan"
                     /gene_synonym="Agc; Agc1; b2b183Clo; cmd; CSPCP; Cspg1"
                     /inference="alignment:Splign:2.1.0"
     exon            1442..1846
                     /gene="Acan"
                     /gene_synonym="Agc; Agc1; b2b183Clo; cmd; CSPCP; Cspg1"
                     /inference="alignment:Splign:2.1.0"
     exon            1847..2021
                     /gene="Acan"
                     /gene_synonym="Agc; Agc1; b2b183Clo; cmd; CSPCP; Cspg1"
                     /inference="alignment:Splign:2.1.0"
     exon            2022..2149
                     /gene="Acan"
                     /gene_synonym="Agc; Agc1; b2b183Clo; cmd; CSPCP; Cspg1"
                     /inference="alignment:Splign:2.1.0"
     exon            2150..2443
                     /gene="Acan"
                     /gene_synonym="Agc; Agc1; b2b183Clo; cmd; CSPCP; Cspg1"
                     /inference="alignment:Splign:2.1.0"
     exon            2444..2659
                     /gene="Acan"
                     /gene_synonym="Agc; Agc1; b2b183Clo; cmd; CSPCP; Cspg1"
                     /inference="alignment:Splign:2.1.0"
     exon            2660..6142
                     /gene="Acan"
                     /gene_synonym="Agc; Agc1; b2b183Clo; cmd; CSPCP; Cspg1"
                     /inference="alignment:Splign:2.1.0"
     exon            6143..6256
                     /gene="Acan"
                     /gene_synonym="Agc; Agc1; b2b183Clo; cmd; CSPCP; Cspg1"
                     /inference="alignment:Splign:2.1.0"
     exon            6257..6415
                     /gene="Acan"
                     /gene_synonym="Agc; Agc1; b2b183Clo; cmd; CSPCP; Cspg1"
                     /inference="alignment:Splign:2.1.0"
     exon            6416..6498
                     /gene="Acan"
                     /gene_synonym="Agc; Agc1; b2b183Clo; cmd; CSPCP; Cspg1"
                     /inference="alignment:Splign:2.1.0"
     exon            6499..6643
                     /gene="Acan"
                     /gene_synonym="Agc; Agc1; b2b183Clo; cmd; CSPCP; Cspg1"
                     /inference="alignment:Splign:2.1.0"
     exon            6644..6826
                     /gene="Acan"
                     /gene_synonym="Agc; Agc1; b2b183Clo; cmd; CSPCP; Cspg1"
                     /inference="alignment:Splign:2.1.0"
     exon            6827..7726
                     /gene="Acan"
                     /gene_synonym="Agc; Agc1; b2b183Clo; cmd; CSPCP; Cspg1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7235..7240
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Acan"
                     /gene_synonym="Agc; Agc1; b2b183Clo; cmd; CSPCP; Cspg1"
                     /note="hexamer: AATAAA"
     polyA_site      7269
                     /gene="Acan"
                     /gene_synonym="Agc; Agc1; b2b183Clo; cmd; CSPCP; Cspg1"
     regulatory      7703..7708
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Acan"
                     /gene_synonym="Agc; Agc1; b2b183Clo; cmd; CSPCP; Cspg1"
                     /note="hexamer: AATAAA"
     polyA_site      7726
                     /gene="Acan"
                     /gene_synonym="Agc; Agc1; b2b183Clo; cmd; CSPCP; Cspg1"
                     /note="major polyA site"
ORIGIN      
        1 cctacctccc cgcggtgcca gaggggctca cagagctgag gacgcgtgcg gaacggctca
       61 aggtccctca ctctctctcc gcatcctcgc cggctagcat ctgacgcagg tgccgaggga
      121 gttcgggcac ctttctgtgt cccttccctt catcgaccct gctccgaaac ctagtcagcc
      181 agccaccact gcggccaccg cccgagggga cttgcggaga agacgccggc gggagaaggg
      241 gttcgcaggg cccccccccc cccgcgcaga gcatccctgc tgcagcgcag caagacccgg
      301 ggctggcagc ccccaggaat ccctagctgc ttcgcaggga taaaggactg aagttcttgg
      361 aggagcgagt ccaactcttc aagctgaact atgaccactt tactcttggt ctttgtgact
      421 ctgagggtca tcgctgcagt gatctcagaa gaagttccag accatgacaa ctcactgagc
      481 gtgagcatcc ctcaaccatc cccattgaag gtcctcctag ggtcttccct caccatcccc
      541 tgctacttca tcgaccccat gcatcctgtg accactgccc cctccactgc ccccctcacc
      601 ccaagaatca agtggagccg tgtttccaag gaaaaggagg tggtactgct ggtggccact
      661 gaaggacagg ttcgagtcaa cagcatctac caagacaagg tgtcgctccc caactatcca
      721 gccatcccca gcgatgctac cttggagatc cagaaccttc gctccaatga ctctgggatc
      781 taccgctgtg aagtgatgca cggcatcgag gacagcgaag ccaccctgga ggtcatagtg
      841 aaaggtattg tgttccacta cagagctatt tccacacgct acaccctgga ctttgatcga
      901 gcacagcggg cttgcctaca gaacagcgcc atcatcgcca ccccagaaca actgcaggct
      961 gcctatgagg atggcttcca ccagtgcgat gcaggctggc tggctgacca gacagtcaga
     1021 taccccatcc acacgccccg ggaaggttgc tatggtgaca aggacgagtt ccctggagtg
     1081 agaacctacg gaatccggga caccaatgag acctatgatg tgtactgctt cgctgaggag
     1141 atggagggtg aggtctttta tgcgacatcc ccagagaaat tcaccttcca ggaggcagcc
     1201 aacgagtgcc ggaggctggg ggcacgcttg gccaccacag gccagctcta cctggcctgg
     1261 cagggcggta tggacatgtg cagcgctggc tggctggcgg accgcagcgt gcgctacccc
     1321 atctccaagg ctcggcccaa ctgcgggggc aacctcctgg gtgtaaggac tgtctatcta
     1381 cacgccaacc agacaggcta tcctgatccc tcatcccgat atgacgccat ctgttacaca
     1441 ggtgaagatt ttgtagacat cccagaaaac ttcttcgggg tgggtggtga agacgacatc
     1501 accatccaga cggtgacctg gccagacctg gagctgcccc tgccccgtaa tgtcacagag
     1561 ggagaggccc tgggcagcgt gatccttaca gcaaagccca tctttgacct gtcccccact
     1621 atctcagagc cgggggaggc cctcacactt gcccctgaag tggggagcac agccttccca
     1681 gaggctgagg agagaactgg agaagccacc agaccctggg gctttcctgc agaagtcaca
     1741 cgtgggccgg actctgccac tgccttcgcc agtgaggacc tggtagtgcg agtgaccatc
     1801 tctccaggtg cagctgaagt ccctggtcag ccccgcttgc cagggggagt tgtattccac
     1861 tatcgcccag gctccaccag atactctctg acatttgagg aggcacagca ggcctgcatg
     1921 cacaccgggg ccgtcattgc ctctcctgag cagctccaag ctgcctatga ggcaggctat
     1981 gagcagtgtg atgctggctg gctgcaggac cagaccgtca gataccccat tgtgagccca
     2041 cgaaccccat gtgtgggtga caaagacagc agcccaggag tcaggaccta cggcgtgcgc
     2101 ccatcatcag aaacctatga tgtctactgc tacgtggaca agcttgaggg ggaagtgttc
     2161 ttcgccacac gcctcgagca gttcaccttc caggaagcgc gggccttctg tgcggctcaa
     2221 aatgccaccc tggcctccac cggccagctc tatgctgcct ggagccaggg tctggacaag
     2281 tgctatgctg gctggctggc agatggcacc ctccgatacc ccatcatcac ccctcggcct
     2341 gcctgtggtg gggacaaacc tggcgtgaga actgtctacc tctaccccaa ccaaaccggc
     2401 ctccctgacc cactgtcaaa gcaccatgcc ttctgcttcc gaggtgtgtc agtggcgccc
     2461 tctccaggag aagaaggggg tagtacaccc acatcaccct ctgacataga ggactggatc
     2521 gtcactcagg tggggcctgg tgtggatgct gtccccttgg agccaaagac aacagaagtg
     2581 ccatatttca ccactgagcc aagaaaacag actgaatggg agccagccta caccccagtg
     2641 ggcacatccc cacagccagg gatccctccc acatggcttc ccaccctccc agcagcagag
     2701 gaacacacag aaagcccctc tgcctctgaa gagccctctg cctcagcagt cccttccacc
     2761 tcagaggagc catacacatc ttcatttgca gtgccgagca tgacagagct gccaggctct
     2821 ggggaggcgt cgggcgcacc tgacctcagt ggtgacttca caggcagtgg agatgcttca
     2881 ggacgccttg actccagtgg gcagccttca gggggcattg aaagtggcct tccctcaggt
     2941 gaccttgact ccagtggcct cagccccaca gtgagctcag gcctgcctgt agaaagtggt
     3001 tctgcctcag gagatggaga agtcccctgg tcccatactc ccacagttgg caggttgccc
     3061 tctggaggtg agagccccga aggctctgcc tctgcctctg gaacaggaga ccttagtggg
     3121 ctgccttcag gaggagaaat tacagaaact tctacttctg gggcagaaga aaccagtgga
     3181 cttccttctg gaggagacgg tctagaaact tctacctctg gagtagatga tgtcagtgga
     3241 attcctactg gaagagaagg tctagagact tctgcctctg gagtagagga cctcagtgga
     3301 cttccttctg gagaagaagg ttcagaaaca tctacctctg gaatagagga catcagtgta
     3361 cttccaactg gaggagaaag tctagaaacc tctgcttctg gagtgggaga cttgagtgga
     3421 cttccctcag gaggagaaag tctagaaaca tctgcttcag gtgcagagga tgtcactcag
     3481 cttcctactg aaagaggagg tctagagact tctgcctctg gagtagaaga catcactgtt
     3541 cttcctactg gaagagaaag tctagaaact tctgcctctg gagtagagga tgtcagtgga
     3601 cttccttctg gaagagaagg tctagagact tctgcctctg gaatagagga cattagtgtg
     3661 tttcctactg aagcagaagg tctggacact tctgcctctg ggggatatgt tagtgggatt
     3721 ccttctggag gagatggtac agaaacctct gcttctggag tagaggatgt gagtggtctt
     3781 ccatctggag gagagggtct agaaacttct gcctctggag tggaagatct tggtccttct
     3841 actagagata gtctagagac atctgcttca ggagtagatg ttactgggtt tccttctgga
     3901 agaggggacc cagagacctc tgtttctggg gtaggtgatg acttcagtgg acttccttct
     3961 ggaaaagaag gcctggagac ctcagcttct ggagctgagg acctcagtgg cttgccctct
     4021 ggaaaagaag acttggtagg gtctgcttct ggggccttgg actttggcaa actacctcct
     4081 ggaactctag gaagtggtca aactccagaa gtaaatggct ttccctctgg atttagtggt
     4141 gagtattctg gagcagacat tggaagtggc ccatcctctg gcctgcctga ctttagtgga
     4201 cttccatctg gctttccaac tgtctccctt gtggacagta ccttagtgga agtgatcaca
     4261 gccaccactt ccagtgaact ggaaggaagg gggaccattg gcatcagtgg ttcaggagaa
     4321 gtatcagggc tgcccctggg tgaattggac agtagtgcgg acattagtgg tctcccttca
     4381 ggaactgaac tcagtggcca agcatctgga tctcctgata gcagtggaga aacatctgga
     4441 ttttttgatg ttagtggaca gccatttggg tcttctggcg tcagcgagga aacatctggg
     4501 attcctgaaa tcagtgggca gccatcaggg actcctgaca ccactgcgac atctggagtg
     4561 actgagctta atgaactgtc ctctggacaa ccagatgtca gtggagatgg gtctggaatt
     4621 ctctttggca gtggacagtc ctctggtata acatctgtga gtggagaaac ctctgggatt
     4681 tctgatctca gtgggcagcc ctcagggttc ccagtgttca gtggaacagc aaccagaacc
     4741 cctgacctgg cttctggcac cataagtggc agtggagagt cttctggcat tacatttgtg
     4801 gacaccagtt ttgttgaagt gacccctacc acatttaggg aagaagaagg gttaggatct
     4861 gtggaactca gtggctttcc ttctggggag acggaactgt ctggcacatc tgggacggtg
     4921 gacgtcagtg aacaatcttc tggagcaatt gattccagtg gactcacatc ccccactcca
     4981 gagttcagtg gcctcccaag tggagtagct gaggtcagtg gtgaattctc tggagttgag
     5041 actgggagca gcttgccctc aggagcattt gatggcagtg gacttgtctc aggtttcccc
     5101 actgtgtctc ttgtagacag aactttggtg gaatctataa ctcaggctcc tactgctcaa
     5161 gaagctggag aaggaccttc gggcattttg gaattcagtg gtgcccattc tgggacacca
     5221 gacatatctg gggagctttc tgggtctctg gacctaagca cattgcagtc tgggcagatg
     5281 gaaaccagca cggagacacc aagctctcca tattttagtg gagacttttc cagcaccact
     5341 gatgtaagtg gagaatccat agctgccaca actggcagtg gggaaagctc tgggcttccg
     5401 gaagttactt taaacacctc agagttagtg gagggtgtga ctgaacccac tgtttcccag
     5461 gaacttggcc atggtccttc tatgacatac actccccggc tctttgaggc cagtggggac
     5521 gcctcagcat ccggggacct tggtggcgct gtaacaaact tcccagggtc tgggatagaa
     5581 gcttcagtcc cagaagccag cagtgacctg tctgcttacc ctgaggctgg tgtgggagtg
     5641 tctgctgccc ctgaggccag cagtaaactg tctgagttcc cagatctgca tggaatcact
     5701 tctgccttcc atgaaacaga tctggaaatg acaaccccaa gcacagaggt aaacagcaac
     5761 ccatggacct ttcaggaagg caccagggag ggatcggccg ctccggaagt gagtggagaa
     5821 tctagcacta cctccgacat agacacaggc acttcagggg tgccttctgc cacacccatg
     5881 gcttctggag acaggactga aatcagcgga gaatggtctg atcacacctc agaggtgaat
     5941 gttgccatca gcagcaccat cacagagtcc gagtgggccc agcctacccg gtaccctaca
     6001 gagacacttc aagaaatcga atccccaaat ccctcatact caggagagga gacccagaca
     6061 gcagaaacaa ccatgtccct gacagatgcc cccaccctct cttcttcaga agggtcaggg
     6121 gagacggagt caaccgttgc agacattgac gagtgcctct caagcccttg tctgaatgga
     6181 gccacctgcg tggatgcctt ggacactttc acatgcttat gccttccgag ctacagaggg
     6241 aacctgtgtg agatcgacca ggagcaatgt gaggaggggt ggactaagtt ccagggtcac
     6301 tgttaccgcc actttcatga ccgagagacc tgggtggatg cggagagacg gtgtcgggag
     6361 cagcagtcac atctgagcag cattgtcact cctgaggaac aggagttcgt caacaaaaat
     6421 gctcaagact accagtggat cggtctgaat gacaggacta tcgaagggga cttccgctgg
     6481 tctgacggac actctctgca atttgagaag tggcgtccaa accagcctga caacttcttt
     6541 gccaccggag aggactgtgt ggtgatgatc tggcatgaga gaggcgaatg gaacgacgtc
     6601 ccctgcaatt accagctgcc cttcacgtgt aaaaagggca ccgtggcctg tggagacccc
     6661 ccagtggtgg agcatgctag aaccctcggg cagaagaaag atcgctacga gatcagctcc
     6721 ctggtgcggt accagtgcac tgagggcttt gtccagcgcc acgtgcccac catccggtgc
     6781 cagcccagcg ggcactggga agagcctcga atcacctgca cagaccccaa cacctacaag
     6841 cacaggctac agaagcggag catgagaccc acacggagga gccgccccag catggcccac
     6901 tgagaggagc ttccataatg tgcccaggat gctgagccca gcggccagcc aggctgaccg
     6961 tgcatcccac ccacatggtg tcttcttgtc gctttttgtc atataaggaa tccattaaag
     7021 aaggaaaaaa aacccacatt gtgtgtgccc cactccccgc atcacccaaa ctgcatctaa
     7081 tttatcccct tctccccccc ccccaaaaaa aaagaaaaat gccaaagcaa ttcactgtaa
     7141 cccgtggact gagtttagag actttttttg atttcccacc gtgcctttcc agggaccagt
     7201 gcagggacag ggtgagaagt aaggggttaa attaaataaa gaagattctt ttttgttgtt
     7261 gtttcctgac tttgcccaag aacagtacaa tggttggtta cttcgcctcc agggagagct
     7321 aggggaccag aggaggagct gaaagaagac aggaccagga agggaggagt agggcggtca
     7381 cagagcttgg aggactcaga agaggcaaag tacagcttag tgtgttggac aaaaggaatt
     7441 gaggtctgtg ccatctgtga gggaaggagc ctggggtagg gacaggctgg ctgaagagaa
     7501 ggcagagccc tctcctaggg acctttcatc tgggtcaacc aacctagcag gtgtcatttc
     7561 tcggctggac tgggtagggg cacttccttc ccaggggtgc tccgccgtcc ccttccctct
     7621 ccccaccccg ggacggtgca ggcaccagtg ttccgtgcac ctatttatat ttttgaaaac
     7681 taaagattat gataattata ataataaaga cattggaaga gatcta
//
